Spg007811 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg007811
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Description1,3-beta-glucan synthase
Locationscaffold4: 9287152 .. 9290665 (-)
RNA-Seq ExpressionSpg007811
SyntenySpg007811
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGGGTTTCAGAAGCTCACGTGTCACTTTTACAGGGTACGTTTCCATTATGGTCATCCTGATATATTTGATAGAATCTTCCACATAACGAGGGGTGGCATCAGTAAAGCTTCTAGAGTCGTTAACTTAAGCGAGGATATATTTGCTGGTATTTCTTATTTCAATTGATGATTGCATGCAGATAGATTCCACCTGTCAATTAATTGTGCTAATCTTAACAGGATATAATTCAACTCTTCGAGGGGGGCTTGTCACACATCATGAGTACATCCAAGTAGGCAAGGGGCGAGATGTGGGCATGAATCAAATATCACTTTTTGAGGCCAAGGTCGCAAATGGGAATGGAGAACAGACACTTAGTCGTGATGTTTATAGGCTTGGACGCCGATTTGACTTCTATAGAATGTTGTCCTTCTACTTCACAACTGTTGGATTCTACTTCAGTAGTATGGTATTATTTCCATTAGCAAGATCTGTTTTCGTACTATTATAATTTATATATAGCTTTGCACTATCCTTGAATAACTGCTCAAAATTTTCCCCTCGAGATCAACACATGGGCTCCTGGTTTTAAATAGGTTAAATATTAGCTCAGGTACCTTTTCTTGTGTTTGGATATCTAACTAATATCTCTTCTCTAGTAGTAGATAGAAACATGTAATTGCTTCTCGTAACTGTTGTATATATGTAAATATTAACATTAAAAAAAATATGTACATATTTGTATTTTGCCTGCAAAGATGCTATATTTTTTACTCAAAAAAAATTGTTGATGCACATATTTCTTCTATAGTCATTTATGTTAAAATGTAGAGGGAGGTAGTTTAGAATTGCATGAATATAATTTTTAGTTGGCTATTTCCAACTTGACTGAATTTGGATACTTGATAGCCTATATTAAGATATCCTGGTAGGCTTCAACTTTGTGGGTAGTTTCTGTGAGGGGCTGAATCATTATCATAGTACGAGTTCTAAGACCAATTAATTCCCTTAGTGGGCAGAAATATCCTTCCAAATTCGGGAAGGTCTACAGTAAATGATTATTCTTTGACTTTCAATTCCCAAATGGTGGTTTCTATTGCCTGTGCACGTTCGTCTTAATAGCAAGGATCATTCTTAGCTTTTGAATTATACATCTCGCAGGTGACTGTGCTGACAGTGTATGTATTTTTATATGGACGTTTGTACATGGTTATGAGTGGAGTTGAAAGGGAGATTCTTGAAAACCCTTCGATACATCAGACTAAGGCTCTTGAAGAGGCTTTGGCTACCCAATCTGTCTTTCAACTAGGCTTGTTGCTGGTACTACCAATGGTTATGGAAATTGGCCTTGAAAAAGGATTTCGCACTGCCCTTGGTGATTTTGTTATCATGCAGCTGCAACTAGCTTCCGTCTTTTTCACATTCCAGCTTGGAACTAAAGCACACTTTTATGGTCGAACAATCTTGCATGGAGGTTCTAAATATCGAGCTACTGGTCGTGGATTTGTTGTTTTTCATGCAAGATTTGCTGATAATTATAGGCAGTACTCACGGAGTCACTTTGTCAAAGGCTTGGAACTGTTTATTCTGCTGCTTGTTTATCAAATCTATGGAGAGTCATATCGTAGTTCAAAACTTTATCTGTTCATCACTTTCTCGATGTGGTTTCTGGTTGCCTCCTGGTTGTTTGCTCCCTTTGTGTTCAATCCATCTGGATTTGATTGGCAAAAGACGGTTGATGACTGGACAGATTGGAAAAGGTGGATGGGAAATCGTGGTGGAATAGGGATCTCCCATGACAAAAGTTGGGAATCTTGGTGGGATGCAGAACAGGAACACCTGAAAAGTACGACTATAAGGGGAAGAATGCTAGAGATAATACTTGCTTTACGGTTCCTCATTTACCAATATGGAATTGTTTACCACCTTGATATTTCTCATCGCGTTAAGAGCTTATTGGTAGGGAATTTTTCTATTATCACTAAGATGTTTTCTCTTAGTGTCCTATTGGAATTACTTTGGATATGTTGCTAATATCTCACTGCTGCATTTCCCGTCAATGGCTTTCCAGGTTTATGGACTTTCTTGGGTAGTCATGTTAATTGCTCTTGTTGTCTTAAAGGTATACCCCTACACAAGGATCTCATACTATTTTCTTACCTTGAAGGCTGATTTTCTTTAACATTTTTACTACTTTAGACTGGTTAATTTGCCTGGTCCGATGAAACTGTATTTTACATTCATTCTTGGTAGATTACTATTCTTGTTTACTGATGTATGTTTATGTTACACGTGGTTACCAGTTGGTTTCAATGGGCAGACGAAAGTTCGGTACCGACTTCCAGCTCATGTTCAGAATACTGAAAGCTCTTCTCTTCCTTGGTTTCATGTCAGTCATGACTGTCTTGTTTGTAGTATGTGGCCTCACAGTATCAGATCTATTTGCAGCTATTCTTGCGTTCTTGCCTACTGGATGGGCCATCCTTCTAGTAAGTTTTGCATTTTTTTTTTTTGAAACAAGTAAGTTTTGCATTTATGTCTTCATATGATATCCGTTCGATTTGAAATAAAATTCTATTGTTCAAAAATAAAAAATTGAATCTGGTCAAAAGATAAGGTTTTTTTTTTTTAATTACAACTTGCTCATGATTATTAATGATTTGCAGACTCTTTTGAGATAGTGCTCGTGCAGGGGCGACCTCCTCCCCTGGCCCTTAGGCTGTTTTTTGCCTTTTTGATTGGAATATAATCCTTTTGATGTTTCTTATTAGAAGGAAAAAAAAATGCTTGTTTTCTCTCCATTTCCTTACCATAGATTTTATCTTTGTTAAAGACAAATTTGAATTCCTAACCAAATTCCAAAAACAAAAACAAGTTTGTGAAAACTACTTATTTTAGTTTTCAAAATTTGACTTGATTTTTGAAAACATGGGAAGTAGTGTTTATAAGCTTAATTTTCAAAAACCAAAAACCGAATGTCAAATGGTTACCAAATGGGATTTAAATACTTCAAACTGTGAATTCAAATCTAAGGATGTGTACTTGTTACCGTTTGATTTTCCTCGACTTTCTCGATCTACCGAGGAAATTAGATGTCAAGGTCATCACAGTTCACTAAACTCTGTTATTCTGACTGTTCCTCTTTGAAAATGAGATTTGACATAGATAAAGCTTGGACATTGTTTGTTTATCACAGCTTTCTTTGTATTAGTTACATGTGAAAGACCCAATACTGATTCATCTTTGTCTCTTTTCAAAGATTGGGCAGGCATGCAGACCGATGATGAAGGGGATAGGATTCTGGGAATCAATAAAAGAGCTTGCAAGAGCTTATGAATACATCATGGGTCTCATAATTTTCATGCCCATAGCCATTTTGTCATGGTTCCCATTTGTATCAGAGTTTCAAACTCGTTTGCTCTTCAATCAAGCATTCAGTAGAGGCCTGCAGATTTCAATGATTCTTTCAGGAAGGAAAGAAACGCCATCCATGATTCTTGCAGGAAAAAAAGAAACGCCATCTACTTGA

mRNA sequence

ATGGATGGGTTTCAGAAGCTCACGTGTCACTTTTACAGGGTACGTTTCCATTATGGTCATCCTGATATATTTGATAGAATCTTCCACATAACGAGGGGTGGCATCAGTAAAGCTTCTAGAGTCGTTAACTTAAGCGAGGATATATTTGCTGGATATAATTCAACTCTTCGAGGGGGGCTTGTCACACATCATGAGTACATCCAAGTAGGCAAGGGGCGAGATGTGGGCATGAATCAAATATCACTTTTTGAGGCCAAGGTCGCAAATGGGAATGGAGAACAGACACTTAGTCGTGATGTTTATAGGCTTGGACGCCGATTTGACTTCTATAGAATGTTGTCCTTCTACTTCACAACTGTTGGATTCTACTTCAGTAGTATGGTGACTGTGCTGACAGTGTATGTATTTTTATATGGACGTTTGTACATGGTTATGAGTGGAGTTGAAAGGGAGATTCTTGAAAACCCTTCGATACATCAGACTAAGGCTCTTGAAGAGGCTTTGGCTACCCAATCTGTCTTTCAACTAGGCTTGTTGCTGGTACTACCAATGGTTATGGAAATTGGCCTTGAAAAAGGATTTCGCACTGCCCTTGGTGATTTTGTTATCATGCAGCTGCAACTAGCTTCCGTCTTTTTCACATTCCAGCTTGGAACTAAAGCACACTTTTATGGTCGAACAATCTTGCATGGAGGTTCTAAATATCGAGCTACTGGTCGTGGATTTGTTGTTTTTCATGCAAGATTTGCTGATAATTATAGGCAGTACTCACGGAGTCACTTTGTCAAAGGCTTGGAACTGTTTATTCTGCTGCTTGTTTATCAAATCTATGGAGAGTCATATCGTAGTTCAAAACTTTATCTGTTCATCACTTTCTCGATGTGGTTTCTGGTTGCCTCCTGGTTGTTTGCTCCCTTTGTGTTCAATCCATCTGGATTTGATTGGCAAAAGACGGTTGATGACTGGACAGATTGGAAAAGGTGGATGGGAAATCGTGGTGGAATAGGGATCTCCCATGACAAAAGTTGGGAATCTTGGTGGGATGCAGAACAGGAACACCTGAAAAGTACGACTATAAGGGGAAGAATGCTAGAGATAATACTTGCTTTACGGTTCCTCATTTACCAATATGGAATTGTTTACCACCTTGATATTTCTCATCGCGTTAAGAGCTTATTGGTTTATGGACTTTCTTGGGTAGTCATGTTAATTGCTCTTGTTGTCTTAAAGTTGGTTTCAATGGGCAGACGAAAGTTCGGTACCGACTTCCAGCTCATGTTCAGAATACTGAAAGCTCTTCTCTTCCTTGGTTTCATGTCAGTCATGACTGTCTTGTTTGTAGTATGTGGCCTCACAGTATCAGATCTATTTGCAGCTATTCTTGCGTTCTTGCCTACTGGATGGGCCATCCTTCTAATTGGGCAGGCATGCAGACCGATGATGAAGGGGATAGGATTCTGGGAATCAATAAAAGAGCTTGCAAGAGCTTATGAATACATCATGGGTCTCATAATTTTCATGCCCATAGCCATTTTGTCATGGTTCCCATTTGTATCAGAGTTTCAAACTCGTTTGCTCTTCAATCAAGCATTCAGTAGAGGCCTGCAGATTTCAATGATTCTTTCAGGAAGGAAAGAAACGCCATCCATGATTCTTGCAGGAAAAAAAGAAACGCCATCTACTTGA

Coding sequence (CDS)

ATGGATGGGTTTCAGAAGCTCACGTGTCACTTTTACAGGGTACGTTTCCATTATGGTCATCCTGATATATTTGATAGAATCTTCCACATAACGAGGGGTGGCATCAGTAAAGCTTCTAGAGTCGTTAACTTAAGCGAGGATATATTTGCTGGATATAATTCAACTCTTCGAGGGGGGCTTGTCACACATCATGAGTACATCCAAGTAGGCAAGGGGCGAGATGTGGGCATGAATCAAATATCACTTTTTGAGGCCAAGGTCGCAAATGGGAATGGAGAACAGACACTTAGTCGTGATGTTTATAGGCTTGGACGCCGATTTGACTTCTATAGAATGTTGTCCTTCTACTTCACAACTGTTGGATTCTACTTCAGTAGTATGGTGACTGTGCTGACAGTGTATGTATTTTTATATGGACGTTTGTACATGGTTATGAGTGGAGTTGAAAGGGAGATTCTTGAAAACCCTTCGATACATCAGACTAAGGCTCTTGAAGAGGCTTTGGCTACCCAATCTGTCTTTCAACTAGGCTTGTTGCTGGTACTACCAATGGTTATGGAAATTGGCCTTGAAAAAGGATTTCGCACTGCCCTTGGTGATTTTGTTATCATGCAGCTGCAACTAGCTTCCGTCTTTTTCACATTCCAGCTTGGAACTAAAGCACACTTTTATGGTCGAACAATCTTGCATGGAGGTTCTAAATATCGAGCTACTGGTCGTGGATTTGTTGTTTTTCATGCAAGATTTGCTGATAATTATAGGCAGTACTCACGGAGTCACTTTGTCAAAGGCTTGGAACTGTTTATTCTGCTGCTTGTTTATCAAATCTATGGAGAGTCATATCGTAGTTCAAAACTTTATCTGTTCATCACTTTCTCGATGTGGTTTCTGGTTGCCTCCTGGTTGTTTGCTCCCTTTGTGTTCAATCCATCTGGATTTGATTGGCAAAAGACGGTTGATGACTGGACAGATTGGAAAAGGTGGATGGGAAATCGTGGTGGAATAGGGATCTCCCATGACAAAAGTTGGGAATCTTGGTGGGATGCAGAACAGGAACACCTGAAAAGTACGACTATAAGGGGAAGAATGCTAGAGATAATACTTGCTTTACGGTTCCTCATTTACCAATATGGAATTGTTTACCACCTTGATATTTCTCATCGCGTTAAGAGCTTATTGGTTTATGGACTTTCTTGGGTAGTCATGTTAATTGCTCTTGTTGTCTTAAAGTTGGTTTCAATGGGCAGACGAAAGTTCGGTACCGACTTCCAGCTCATGTTCAGAATACTGAAAGCTCTTCTCTTCCTTGGTTTCATGTCAGTCATGACTGTCTTGTTTGTAGTATGTGGCCTCACAGTATCAGATCTATTTGCAGCTATTCTTGCGTTCTTGCCTACTGGATGGGCCATCCTTCTAATTGGGCAGGCATGCAGACCGATGATGAAGGGGATAGGATTCTGGGAATCAATAAAAGAGCTTGCAAGAGCTTATGAATACATCATGGGTCTCATAATTTTCATGCCCATAGCCATTTTGTCATGGTTCCCATTTGTATCAGAGTTTCAAACTCGTTTGCTCTTCAATCAAGCATTCAGTAGAGGCCTGCAGATTTCAATGATTCTTTCAGGAAGGAAAGAAACGCCATCCATGATTCTTGCAGGAAAAAAAGAAACGCCATCTACTTGA

Protein sequence

MDGFQKLTCHFYRVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGGLVTHHEYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYFSSMVTVLTVYVFLYGRLYMVMSGVEREILENPSIHQTKALEEALATQSVFQLGLLLVLPMVMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVVFHARFADNYRQYSRSHFVKGLELFILLLVYQIYGESYRSSKLYLFITFSMWFLVASWLFAPFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDAEQEHLKSTTIRGRMLEIILALRFLIYQYGIVYHLDISHRVKSLLVYGLSWVVMLIALVVLKLVSMGRRKFGTDFQLMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAILAFLPTGWAILLIGQACRPMMKGIGFWESIKELARAYEYIMGLIIFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMILSGRKETPSMILAGKKETPST
Homology
BLAST of Spg007811 vs. NCBI nr
Match: XP_023530416.1 (callose synthase 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1046.2 bits (2704), Expect = 1.0e-301
Identity = 530/557 (95.15%), Postives = 546/557 (98.03%), Query Frame = 0

Query: 5    QKLTCHFYRVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGGLVTHH 64
            Q++  +  RVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGG VTHH
Sbjct: 1371 QRILANPLRVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGGFVTHH 1430

Query: 65   EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYF 124
            EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDF+RMLSFYFTTVGFYF
Sbjct: 1431 EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFFRMLSFYFTTVGFYF 1490

Query: 125  SSMVTVLTVYVFLYGRLYMVMSGVEREILENPSIHQTKALEEALATQSVFQLGLLLVLPM 184
            SSMVTVLTVYVFLYGRLYMVMSGVEREIL NPSI QTKALEEALATQSVFQLGLLLVLPM
Sbjct: 1491 SSMVTVLTVYVFLYGRLYMVMSGVEREILGNPSIRQTKALEEALATQSVFQLGLLLVLPM 1550

Query: 185  VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 244
            VMEIGLEKGFRTALGDF+IMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV
Sbjct: 1551 VMEIGLEKGFRTALGDFIIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 1610

Query: 245  FHARFADNYRQYSRSHFVKGLELFILLLVYQIYGESYRSSKLYLFITFSMWFLVASWLFA 304
            FHA+FADNYRQYSRSHFVKGLEL ILLLVYQIYG+SYRSSKLYLFITFSMWFLVASWLFA
Sbjct: 1611 FHAKFADNYRQYSRSHFVKGLELLILLLVYQIYGQSYRSSKLYLFITFSMWFLVASWLFA 1670

Query: 305  PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDAEQEHLKSTTIRGRML 364
            PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWD EQEHLKSTTIRGR+L
Sbjct: 1671 PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDGEQEHLKSTTIRGRVL 1730

Query: 365  EIILALRFLIYQYGIVYHLDISHRVKSLLVYGLSWVVMLIALVVLKLVSMGRRKFGTDFQ 424
            EIILALRFLI+QYGIVYHLDISHR+KSLLVYGLSWVVM+IALVVLKLVSMGRRKFGTDFQ
Sbjct: 1731 EIILALRFLIFQYGIVYHLDISHRIKSLLVYGLSWVVMVIALVVLKLVSMGRRKFGTDFQ 1790

Query: 425  LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAILAFLPTGWAILLIGQACRPMMKGI 484
            LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAI+AFLPTGWAI+LIGQACRPMMKGI
Sbjct: 1791 LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAIIAFLPTGWAIVLIGQACRPMMKGI 1850

Query: 485  GFWESIKELARAYEYIMGLIIFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMILSGR 544
            GFWESIKELARAYEYIMGLIIFMP AILSWFPFVSEFQTRLLFNQAFSRGLQISMIL+G+
Sbjct: 1851 GFWESIKELARAYEYIMGLIIFMPTAILSWFPFVSEFQTRLLFNQAFSRGLQISMILAGK 1910

Query: 545  KETPSMILAGKKETPST 562
            KETPSMI+ GKKETPST
Sbjct: 1911 KETPSMIITGKKETPST 1927

BLAST of Spg007811 vs. NCBI nr
Match: XP_022989096.1 (callose synthase 7-like [Cucurbita maxima])

HSP 1 Score: 1041.6 bits (2692), Expect = 2.5e-300
Identity = 526/557 (94.43%), Postives = 544/557 (97.67%), Query Frame = 0

Query: 5    QKLTCHFYRVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGGLVTHH 64
            Q++  +  RVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGG VTHH
Sbjct: 1372 QRILANPLRVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGGFVTHH 1431

Query: 65   EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYF 124
            EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDF+RMLSFYFTTVGFYF
Sbjct: 1432 EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFFRMLSFYFTTVGFYF 1491

Query: 125  SSMVTVLTVYVFLYGRLYMVMSGVEREILENPSIHQTKALEEALATQSVFQLGLLLVLPM 184
            SSMVTVLTVYVFLYGRLYMVMSGVEREIL NPSI QTKALEEALATQSVFQLGLLLVLPM
Sbjct: 1492 SSMVTVLTVYVFLYGRLYMVMSGVEREILGNPSIRQTKALEEALATQSVFQLGLLLVLPM 1551

Query: 185  VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 244
            VMEIGLEKGFRTALGDF+IMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV
Sbjct: 1552 VMEIGLEKGFRTALGDFIIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 1611

Query: 245  FHARFADNYRQYSRSHFVKGLELFILLLVYQIYGESYRSSKLYLFITFSMWFLVASWLFA 304
            FHA+FADNYRQYSRSHFVKGLEL ILLLVYQIYG+SYRSSKLYLFITFSMWFLVASWLFA
Sbjct: 1612 FHAKFADNYRQYSRSHFVKGLELLILLLVYQIYGQSYRSSKLYLFITFSMWFLVASWLFA 1671

Query: 305  PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDAEQEHLKSTTIRGRML 364
            PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWD EQEHLKSTTIRGR+L
Sbjct: 1672 PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDGEQEHLKSTTIRGRVL 1731

Query: 365  EIILALRFLIYQYGIVYHLDISHRVKSLLVYGLSWVVMLIALVVLKLVSMGRRKFGTDFQ 424
            EIILALRFLI+QYGIVYHLDISHR+KSLLVYGLSWVVM+I LVVLKLVSMGRRKFGTDFQ
Sbjct: 1732 EIILALRFLIFQYGIVYHLDISHRIKSLLVYGLSWVVMVIVLVVLKLVSMGRRKFGTDFQ 1791

Query: 425  LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAILAFLPTGWAILLIGQACRPMMKGI 484
            LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAI+AFLPTGWAI+LIGQACRPMMKGI
Sbjct: 1792 LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAIIAFLPTGWAIVLIGQACRPMMKGI 1851

Query: 485  GFWESIKELARAYEYIMGLIIFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMILSGR 544
            G WESIKELARAYEYIMGLIIFMP A+LSWFPFVSEFQTRLLFNQAFSRGLQISMIL+G+
Sbjct: 1852 GLWESIKELARAYEYIMGLIIFMPTAVLSWFPFVSEFQTRLLFNQAFSRGLQISMILAGK 1911

Query: 545  KETPSMILAGKKETPST 562
            KETPSM++ GKKETPST
Sbjct: 1912 KETPSMVITGKKETPST 1928

BLAST of Spg007811 vs. NCBI nr
Match: KAG6589303.1 (Callose synthase 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1041.2 bits (2691), Expect = 3.3e-300
Identity = 527/556 (94.78%), Postives = 544/556 (97.84%), Query Frame = 0

Query: 5    QKLTCHFYRVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGGLVTHH 64
            Q++  +  RVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGG VTHH
Sbjct: 1372 QRILANPLRVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGGFVTHH 1431

Query: 65   EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYF 124
            EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDF+RMLSFYFTTVGFYF
Sbjct: 1432 EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFFRMLSFYFTTVGFYF 1491

Query: 125  SSMVTVLTVYVFLYGRLYMVMSGVEREILENPSIHQTKALEEALATQSVFQLGLLLVLPM 184
            SSMVTVLTVYVFLYGRLYMVMSGVEREIL NPSI QTKALEEALATQSVFQLGLLLVLPM
Sbjct: 1492 SSMVTVLTVYVFLYGRLYMVMSGVEREILGNPSIRQTKALEEALATQSVFQLGLLLVLPM 1551

Query: 185  VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 244
            VMEIGLEKGFRTALGDF+IMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV
Sbjct: 1552 VMEIGLEKGFRTALGDFIIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 1611

Query: 245  FHARFADNYRQYSRSHFVKGLELFILLLVYQIYGESYRSSKLYLFITFSMWFLVASWLFA 304
            FHA+FADNYRQYSRSHFVKGLEL ILLLVYQIYG+SYRSSKLYLFITFSMWFLVASWLFA
Sbjct: 1612 FHAKFADNYRQYSRSHFVKGLELLILLLVYQIYGQSYRSSKLYLFITFSMWFLVASWLFA 1671

Query: 305  PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDAEQEHLKSTTIRGRML 364
            PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWD EQEHLKSTTIRGR+L
Sbjct: 1672 PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDGEQEHLKSTTIRGRVL 1731

Query: 365  EIILALRFLIYQYGIVYHLDISHRVKSLLVYGLSWVVMLIALVVLKLVSMGRRKFGTDFQ 424
            EIILALRFLI+QYGIVYHLDISHR+KSLLVYGLSWVVM+IALVVLKLVSMGRRKFGTDFQ
Sbjct: 1732 EIILALRFLIFQYGIVYHLDISHRIKSLLVYGLSWVVMVIALVVLKLVSMGRRKFGTDFQ 1791

Query: 425  LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAILAFLPTGWAILLIGQACRPMMKGI 484
            LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAI+AFLPTGWAI+LIGQACRPMMKGI
Sbjct: 1792 LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAIIAFLPTGWAIVLIGQACRPMMKGI 1851

Query: 485  GFWESIKELARAYEYIMGLIIFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMILSGR 544
            GFWESIKELARAYEYIMGL+IFMP AILSWFPFVSEFQTRLLFNQAFSRGLQISMIL+G+
Sbjct: 1852 GFWESIKELARAYEYIMGLVIFMPTAILSWFPFVSEFQTRLLFNQAFSRGLQISMILAGK 1911

Query: 545  KETPSMILAGKKETPS 561
            KETPSMI+ GKKET S
Sbjct: 1912 KETPSMIITGKKETTS 1927

BLAST of Spg007811 vs. NCBI nr
Match: XP_022930628.1 (callose synthase 7-like [Cucurbita moschata])

HSP 1 Score: 1038.9 bits (2685), Expect = 1.6e-299
Identity = 526/556 (94.60%), Postives = 543/556 (97.66%), Query Frame = 0

Query: 5    QKLTCHFYRVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGGLVTHH 64
            Q++  +  RVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGG VTHH
Sbjct: 1372 QRILANPLRVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGGFVTHH 1431

Query: 65   EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYF 124
            EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDF+RMLSFYFTTVGFYF
Sbjct: 1432 EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFFRMLSFYFTTVGFYF 1491

Query: 125  SSMVTVLTVYVFLYGRLYMVMSGVEREILENPSIHQTKALEEALATQSVFQLGLLLVLPM 184
            SSMVTVLTVYVFLYGRLYMVMSGVEREIL NPSI QTKALEEALATQSVFQLGLLLVLPM
Sbjct: 1492 SSMVTVLTVYVFLYGRLYMVMSGVEREILGNPSIRQTKALEEALATQSVFQLGLLLVLPM 1551

Query: 185  VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 244
            VMEIGLEKGFRTALGDF+IMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV
Sbjct: 1552 VMEIGLEKGFRTALGDFIIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 1611

Query: 245  FHARFADNYRQYSRSHFVKGLELFILLLVYQIYGESYRSSKLYLFITFSMWFLVASWLFA 304
            FHA+FADNYRQYSRSHFVKGLEL ILLLVYQIYG+SYRSSKLYLFITFSMWFLVASWLFA
Sbjct: 1612 FHAKFADNYRQYSRSHFVKGLELLILLLVYQIYGQSYRSSKLYLFITFSMWFLVASWLFA 1671

Query: 305  PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDAEQEHLKSTTIRGRML 364
            PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWD EQEHLKSTTIRGR+L
Sbjct: 1672 PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDGEQEHLKSTTIRGRVL 1731

Query: 365  EIILALRFLIYQYGIVYHLDISHRVKSLLVYGLSWVVMLIALVVLKLVSMGRRKFGTDFQ 424
            EIILALRFLI+QYGIVYHLDISHR+KSLLVYGLSWVVM+IALVVLKLVSMGRRKFGTDFQ
Sbjct: 1732 EIILALRFLIFQYGIVYHLDISHRIKSLLVYGLSWVVMVIALVVLKLVSMGRRKFGTDFQ 1791

Query: 425  LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAILAFLPTGWAILLIGQACRPMMKGI 484
            LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAI+AFLPTGWAI+LIGQACRPMMKGI
Sbjct: 1792 LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAIIAFLPTGWAIVLIGQACRPMMKGI 1851

Query: 485  GFWESIKELARAYEYIMGLIIFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMILSGR 544
            GFWESIKELARAYEYIMGL+I MP AILSWFPFVSEFQTRLLFNQAFSRGLQISMIL+G+
Sbjct: 1852 GFWESIKELARAYEYIMGLVILMPTAILSWFPFVSEFQTRLLFNQAFSRGLQISMILAGK 1911

Query: 545  KETPSMILAGKKETPS 561
            KETPSMI+  KKETPS
Sbjct: 1912 KETPSMIITEKKETPS 1927

BLAST of Spg007811 vs. NCBI nr
Match: XP_022148041.1 (callose synthase 7 isoform X1 [Momordica charantia])

HSP 1 Score: 1036.6 bits (2679), Expect = 8.0e-299
Identity = 529/545 (97.06%), Postives = 537/545 (98.53%), Query Frame = 0

Query: 5    QKLTCHFYRVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGGLVTHH 64
            Q++  +  RVRFHYGHPDIFDRIFHITRGGISKASRV+NLSEDIFAGYNSTLRGG VTHH
Sbjct: 1377 QRILANPLRVRFHYGHPDIFDRIFHITRGGISKASRVINLSEDIFAGYNSTLRGGFVTHH 1436

Query: 65   EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYF 124
            EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFYRM+SFYFTTVGFYF
Sbjct: 1437 EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFYRMMSFYFTTVGFYF 1496

Query: 125  SSMVTVLTVYVFLYGRLYMVMSGVEREILENPSIHQTKALEEALATQSVFQLGLLLVLPM 184
            SSMVTVLTVYVFLYGRLYMVMSGVEREILENPSI QTKALEEALATQSVFQLGLLLVLPM
Sbjct: 1497 SSMVTVLTVYVFLYGRLYMVMSGVEREILENPSIRQTKALEEALATQSVFQLGLLLVLPM 1556

Query: 185  VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 244
            VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV
Sbjct: 1557 VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 1616

Query: 245  FHARFADNYRQYSRSHFVKGLELFILLLVYQIYGESYRSSKLYLFITFSMWFLVASWLFA 304
            FHA+FADNYRQYSRSHFVKGLELFILLLVYQIYGESYRSS+LYLFITFSMWFLVASWLFA
Sbjct: 1617 FHAKFADNYRQYSRSHFVKGLELFILLLVYQIYGESYRSSRLYLFITFSMWFLVASWLFA 1676

Query: 305  PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDAEQEHLKSTTIRGRML 364
            PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWD EQEHLKSTTIRGR+L
Sbjct: 1677 PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDGEQEHLKSTTIRGRVL 1736

Query: 365  EIILALRFLIYQYGIVYHLDISHRVKSLLVYGLSWVVMLIALVVLKLVSMGRRKFGTDFQ 424
            EIILALRFLIYQYGIVYHLDISHRVKSLLVYGLSWVVMLIALVVLKLVSMGRRKFGTDFQ
Sbjct: 1737 EIILALRFLIYQYGIVYHLDISHRVKSLLVYGLSWVVMLIALVVLKLVSMGRRKFGTDFQ 1796

Query: 425  LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAILAFLPTGWAILLIGQACRPMMKGI 484
            LMFRILKALLFLGFMSVMTVLFVV GLTVSDLFAAILAFLPTGWAILLIGQACRPMMKGI
Sbjct: 1797 LMFRILKALLFLGFMSVMTVLFVVGGLTVSDLFAAILAFLPTGWAILLIGQACRPMMKGI 1856

Query: 485  GFWESIKELARAYEYIMGLIIFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMILSGR 544
            GFWESIKELARAYEYIMGLIIFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMILSGR
Sbjct: 1857 GFWESIKELARAYEYIMGLIIFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMILSGR 1916

Query: 545  KETPS 550
            KETPS
Sbjct: 1917 KETPS 1921

BLAST of Spg007811 vs. ExPASy Swiss-Prot
Match: Q9SHJ3 (Callose synthase 7 OS=Arabidopsis thaliana OX=3702 GN=CALS7 PE=3 SV=3)

HSP 1 Score: 931.8 bits (2407), Expect = 3.7e-270
Identity = 460/557 (82.59%), Postives = 513/557 (92.10%), Query Frame = 0

Query: 5    QKLTCHFYRVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGGLVTHH 64
            Q++  +  RVRFHYGHPDIFDRIFHITRGGISKAS+++NLSEDIFAGYNSTLRGG VTHH
Sbjct: 1385 QRVLANPLRVRFHYGHPDIFDRIFHITRGGISKASKIINLSEDIFAGYNSTLRGGYVTHH 1444

Query: 65   EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYF 124
            EYIQ GKGRDVGMNQIS FEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYF
Sbjct: 1445 EYIQAGKGRDVGMNQISFFEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYF 1504

Query: 125  SSMVTVLTVYVFLYGRLYMVMSGVEREILENPSIHQTKALEEALATQSVFQLGLLLVLPM 184
            SSM+TVLTVYVFLYGRLY+V+SG+E+ IL++ S+H++ ALE+ALA QSVFQLG L+VLPM
Sbjct: 1505 SSMITVLTVYVFLYGRLYLVLSGLEKNILQSASVHESNALEQALAAQSVFQLGFLMVLPM 1564

Query: 185  VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 244
            VMEIGLEKGFRTALGDF+IMQLQLASVFFTFQLGTKAH++GRTILHGGSKYRATGRGFVV
Sbjct: 1565 VMEIGLEKGFRTALGDFIIMQLQLASVFFTFQLGTKAHYFGRTILHGGSKYRATGRGFVV 1624

Query: 245  FHARFADNYRQYSRSHFVKGLELFILLLVYQIYGESYRSSKLYLFITFSMWFLVASWLFA 304
            FHA+FA+NYR YSRSHFVKGLEL ILL+VYQ+YG SYRSS  Y++ITFSMWFLV SWLFA
Sbjct: 1625 FHAKFAENYRLYSRSHFVKGLELVILLVVYQVYGTSYRSSSTYMYITFSMWFLVTSWLFA 1684

Query: 305  PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDAEQEHLKSTTIRGRML 364
            PF+FNPSGF+WQKTVDDWTDWKRWMGNRGGIGI  DKSWESWWD EQEHLK T +RGR+L
Sbjct: 1685 PFIFNPSGFEWQKTVDDWTDWKRWMGNRGGIGIVLDKSWESWWDIEQEHLKHTNLRGRVL 1744

Query: 365  EIILALRFLIYQYGIVYHLDISHRVKSLLVYGLSWVVMLIALVVLKLVSMGRRKFGTDFQ 424
            EI+LALRFL+YQYGIVYHL+I+ R  + LVYGLSW ++L  L+VLK+VSMGRRKFGTDFQ
Sbjct: 1745 EILLALRFLLYQYGIVYHLNIARRHTTFLVYGLSWAILLSVLLVLKMVSMGRRKFGTDFQ 1804

Query: 425  LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAILAFLPTGWAILLIGQACRPMMKGI 484
            +MFRILKALLFLGF+SVMTVLFVVCGLT+SDLFA+ILAFLPTGWAILLIGQA R + KG+
Sbjct: 1805 VMFRILKALLFLGFLSVMTVLFVVCGLTISDLFASILAFLPTGWAILLIGQALRSVFKGL 1864

Query: 485  GFWESIKELARAYEYIMGLIIFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMILSGR 544
            GFW+S+KEL RAYEYIMGL+IF PIA+LSWFPFVSEFQTRLLFNQAFSRGLQISMIL+G+
Sbjct: 1865 GFWDSVKELGRAYEYIMGLVIFTPIAVLSWFPFVSEFQTRLLFNQAFSRGLQISMILAGK 1924

Query: 545  KETPSMILAGKKETPST 562
            K+         KETPST
Sbjct: 1925 KD---------KETPST 1932

BLAST of Spg007811 vs. ExPASy Swiss-Prot
Match: Q9LYS6 (Putative callose synthase 6 OS=Arabidopsis thaliana OX=3702 GN=CALS6 PE=3 SV=2)

HSP 1 Score: 877.5 bits (2266), Expect = 8.2e-254
Identity = 435/542 (80.26%), Postives = 492/542 (90.77%), Query Frame = 0

Query: 5    QKLTCHFYRVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGGLVTHH 64
            Q++  +  RVRFHYGHPDIFDRIFHITRGG+SKAS+V+NLSEDIF G+NSTLRGG VTHH
Sbjct: 1373 QRILANPLRVRFHYGHPDIFDRIFHITRGGVSKASKVINLSEDIFGGFNSTLRGGYVTHH 1432

Query: 65   EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYF 124
            EYIQVGKGRDVG+N IS+FEAKVANGNGEQTLSRDVYRLG RFDFYRMLSFYFTT+GFYF
Sbjct: 1433 EYIQVGKGRDVGLNPISIFEAKVANGNGEQTLSRDVYRLGHRFDFYRMLSFYFTTIGFYF 1492

Query: 125  SSMVTVLTVYVFLYGRLYMVMSGVEREILENPSIHQTKALEEALATQSVFQLGLLLVLPM 184
            SSM+TVLTVY FLYGR+YMVMSG+E+EIL   S +Q +ALE+ALATQS+FQLG L+VLPM
Sbjct: 1493 SSMLTVLTVYAFLYGRMYMVMSGLEKEILRLASPNQLEALEQALATQSIFQLGFLMVLPM 1552

Query: 185  VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 244
            VMEIGLE GFR+A+ DF IMQLQLASVFFTFQLGTK+H+YGRTILHGGSKYR TGRGFVV
Sbjct: 1553 VMEIGLEHGFRSAIVDFFIMQLQLASVFFTFQLGTKSHYYGRTILHGGSKYRPTGRGFVV 1612

Query: 245  FHARFADNYRQYSRSHFVKGLELFILLLVYQIYGESYRSSKLYLFITFSMWFLVASWLFA 304
            FHA+FA+NYR YSRSHFVKGLEL +LL+VYQIYG SYRSS LYL+IT SMWF+V SWLFA
Sbjct: 1613 FHAKFAENYRLYSRSHFVKGLELLLLLVVYQIYGHSYRSSNLYLYITVSMWFMVGSWLFA 1672

Query: 305  PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDAEQEHLKSTTIRGRML 364
            PF+FNPSGF+WQKTVDDWTDWKRW+G+RGGIGI  +KSWESWW+ EQEHLK T+IRGR+L
Sbjct: 1673 PFIFNPSGFEWQKTVDDWTDWKRWLGDRGGIGIPVEKSWESWWNVEQEHLKHTSIRGRIL 1732

Query: 365  EIILALRFLIYQYGIVYHLDISHRVKSLLVYGLSWVVMLIALVVLKLVSMGRRKFGTDFQ 424
            EI LALRF IYQYGIVY L+IS R KS LVYGLSWVV+L +L+VLK+VSMGRR+FGTDFQ
Sbjct: 1733 EITLALRFFIYQYGIVYQLNISQRSKSFLVYGLSWVVLLTSLLVLKMVSMGRRRFGTDFQ 1792

Query: 425  LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAILAFLPTGWAILLIGQACRPMMKGI 484
            LMFRILKALLFLGF+SVMT+LFVV  LT++DL A++LAFLPTGWAILLIGQ  R  +K +
Sbjct: 1793 LMFRILKALLFLGFLSVMTILFVVFKLTLTDLSASVLAFLPTGWAILLIGQVLRSPIKAL 1852

Query: 485  GFWESIKELARAYEYIMGLIIFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMILSGR 544
            G W+S+KEL RAYE IMGL+IF PIA+LSWFP VSEFQ RLLFNQAFSRGLQISMIL+GR
Sbjct: 1853 GVWDSVKELGRAYENIMGLVIFAPIAVLSWFPIVSEFQARLLFNQAFSRGLQISMILAGR 1912

Query: 545  KE 547
            K+
Sbjct: 1913 KD 1914

BLAST of Spg007811 vs. ExPASy Swiss-Prot
Match: Q9LXT9 (Callose synthase 3 OS=Arabidopsis thaliana OX=3702 GN=CALS3 PE=3 SV=3)

HSP 1 Score: 770.4 bits (1988), Expect = 1.4e-221
Identity = 362/545 (66.42%), Postives = 457/545 (83.85%), Query Frame = 0

Query: 5    QKLTCHFYRVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGGLVTHH 64
            Q+L  +  RVRFHYGHPD+FDR+FH+TRGG+SKAS+V+NLSEDIFAG+NSTLR G VTHH
Sbjct: 1406 QRLLANPLRVRFHYGHPDVFDRLFHLTRGGVSKASKVINLSEDIFAGFNSTLREGNVTHH 1465

Query: 65   EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYF 124
            EYIQVGKGRDVG+NQIS+FEAK+ANGNGEQTLSRD+YRLG RFDF+RM+S YFTTVGFYF
Sbjct: 1466 EYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRDIYRLGHRFDFFRMMSCYFTTVGFYF 1525

Query: 125  SSMVTVLTVYVFLYGRLYMVMSGVEREILENPSIHQTKALEEALATQSVFQLGLLLVLPM 184
            S+++TVLTVY+FLYGRLY+V+SG+E+ +     I     L+ ALA+QS  Q+G L+ LPM
Sbjct: 1526 STLITVLTVYIFLYGRLYLVLSGLEQGLSTQKGIRDNTPLQIALASQSFVQIGFLMALPM 1585

Query: 185  VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 244
            +MEIGLE+GFRTAL +FV+MQLQLA VFFTF LGTK H+YGRT+LHGG+KYR+TGRGFVV
Sbjct: 1586 LMEIGLERGFRTALSEFVLMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAKYRSTGRGFVV 1645

Query: 245  FHARFADNYRQYSRSHFVKGLELFILLLVYQIYGESYRSSKLYLFITFSMWFLVASWLFA 304
            FHA+FADNYR YSRSHFVKGLE+ +LL+VYQI+G +YR    YL IT SMWF+V +WLFA
Sbjct: 1646 FHAKFADNYRLYSRSHFVKGLEMMLLLVVYQIFGSAYRGVLAYLLITISMWFMVGTWLFA 1705

Query: 305  PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDAEQEHLKSTTIRGRML 364
            PF+FNPSGF+WQK VDDWTDW +W+ N GGIG+  +KSWESWW+ EQEHL+ +  RG ++
Sbjct: 1706 PFLFNPSGFEWQKIVDDWTDWNKWINNIGGIGVPAEKSWESWWEEEQEHLRYSGKRGIVV 1765

Query: 365  EIILALRFLIYQYGIVYHLDISHRVKSLLVYGLSWVVMLIALVVLKLVSMGRRKFGTDFQ 424
            EI+LALRF IYQYG+VYHL I+ + K+ LVYG+SW+V+ + L V+K VS+GRR+F   FQ
Sbjct: 1766 EILLALRFFIYQYGLVYHLTITEKTKNFLVYGVSWLVIFLILFVMKTVSVGRRRFSASFQ 1825

Query: 425  LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAILAFLPTGWAILLIGQACRPMMKGI 484
            LMFR++K L+F+ F++++ +L  +  +T+ D+   ILAF+PTGW +LLI QAC+P++   
Sbjct: 1826 LMFRLIKGLIFMTFIAIIVILITLAHMTIQDIIVCILAFMPTGWGMLLIAQACKPVVHRA 1885

Query: 485  GFWESIKELARAYEYIMGLIIFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMILSGR 544
            GFW S++ LAR YE +MGL++F P+A L+WFPFVSEFQTR+LFNQAFSRGLQIS IL G 
Sbjct: 1886 GFWGSVRTLARGYEIVMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGH 1945

Query: 545  KETPS 550
            ++  S
Sbjct: 1946 RKDRS 1950

BLAST of Spg007811 vs. ExPASy Swiss-Prot
Match: Q9AUE0 (Callose synthase 1 OS=Arabidopsis thaliana OX=3702 GN=CALS1 PE=1 SV=2)

HSP 1 Score: 748.4 bits (1931), Expect = 5.7e-215
Identity = 359/546 (65.75%), Postives = 451/546 (82.60%), Query Frame = 0

Query: 5    QKLTCHFYRVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGGLVTHH 64
            Q++     +VRFHYGHPDIFDR+FH+TRGGI KAS+V+NLSEDIFAG+NSTLR G VTHH
Sbjct: 1400 QRVLASPLKVRFHYGHPDIFDRLFHLTRGGICKASKVINLSEDIFAGFNSTLREGNVTHH 1459

Query: 65   EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYF 124
            EYIQVGKGRDVG+NQIS+FEAK+ANGNGEQTLSRD+YRLG RFDF+RMLS YFTT+GFYF
Sbjct: 1460 EYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRDLYRLGHRFDFFRMLSCYFTTIGFYF 1519

Query: 125  SSMVTVLTVYVFLYGRLYMVMSGVEREILENPSIHQTKALEEALATQSVFQLGLLLVLPM 184
            S+M+TVLTVYVFLYGRLY+V+SG+E  +    +    K LE ALA+QS  Q+G L+ LPM
Sbjct: 1520 STMLTVLTVYVFLYGRLYLVLSGLEEGLSSQRAFRNNKPLEAALASQSFVQIGFLMALPM 1579

Query: 185  VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 244
            +MEIGLE+GF  AL +FV+MQLQLASVFFTFQLGTK H+YGRT+ HGG++YR TGRGFVV
Sbjct: 1580 MMEIGLERGFHNALIEFVLMQLQLASVFFTFQLGTKTHYYGRTLFHGGAEYRGTGRGFVV 1639

Query: 245  FHARFADNYRQYSRSHFVKGLELFILLLVYQIYGESYRSSKLYLFITFSMWFLVASWLFA 304
            FHA+FA+NYR YSRSHFVKG+EL ILLLVYQI+G+SYR    Y+ IT S+WF+V +WLFA
Sbjct: 1640 FHAKFAENYRFYSRSHFVKGIELMILLLVYQIFGQSYRGVVTYILITVSIWFMVVTWLFA 1699

Query: 305  PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDAEQEHLKSTTIRGRML 364
            PF+FNPSGF+WQK VDDWTDW +W+ NRGGIG+  +KSWESWW+ E EHL+ + +RG  L
Sbjct: 1700 PFLFNPSGFEWQKIVDDWTDWNKWIYNRGGIGVPPEKSWESWWEKELEHLRHSGVRGITL 1759

Query: 365  EIILALRFLIYQYGIVYHLD-ISHRVKSLLVYGLSWVVMLIALVVLKLVSMGRRKFGTDF 424
            EI LALRF I+QYG+VYHL     + +S  VYG SW V+L  L+++K + +GRR+F T+F
Sbjct: 1760 EIFLALRFFIFQYGLVYHLSTFKGKNQSFWVYGASWFVILFILLIVKGLGVGRRRFSTNF 1819

Query: 425  QLMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAILAFLPTGWAILLIGQACRPMMKG 484
            QL+FRI+K L+FL F++++     +  +T+ DLF  +LAF+PTGW +LLI QAC+P+++ 
Sbjct: 1820 QLLFRIIKGLVFLTFVAILITFLALPLITIKDLFICMLAFMPTGWGMLLIAQACKPLIQQ 1879

Query: 485  IGFWESIKELARAYEYIMGLIIFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMILSG 544
            +G W S++ LAR YE +MGL++F P+A L+WFPFVSEFQTR+LFNQAFSRGLQIS IL G
Sbjct: 1880 LGIWSSVRTLARGYEIVMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGG 1939

Query: 545  RKETPS 550
            +++  S
Sbjct: 1940 QRKDRS 1945

BLAST of Spg007811 vs. ExPASy Swiss-Prot
Match: Q3B724 (Callose synthase 5 OS=Arabidopsis thaliana OX=3702 GN=CALS5 PE=1 SV=1)

HSP 1 Score: 743.0 bits (1917), Expect = 2.4e-213
Identity = 367/547 (67.09%), Postives = 444/547 (81.17%), Query Frame = 0

Query: 5    QKLTCHFYRVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGGLVTHH 64
            Q++     +VRFHYGHPD+FDRIFHITRGGISKASR +NLSEDIFAG+NSTLR G VTHH
Sbjct: 1375 QRVLASPLKVRFHYGHPDVFDRIFHITRGGISKASRGINLSEDIFAGFNSTLRRGNVTHH 1434

Query: 65   EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYF 124
            EYIQVGKGRDVG+NQISLFEAKVA GNGEQTLSRD+YRLG RFDF+RM+S YFTTVGFY 
Sbjct: 1435 EYIQVGKGRDVGLNQISLFEAKVACGNGEQTLSRDLYRLGHRFDFFRMMSCYFTTVGFYI 1494

Query: 125  SSMVTVLTVYVFLYGRLYMVMSGVEREILENPSIHQTKALEEALATQSVFQLGLLLVLPM 184
            SSM+ VLTVY FLYGRLY+ +SGVE  I++  +     +L+ A+A+QSV QLGLL+ LPM
Sbjct: 1495 SSMIVVLTVYAFLYGRLYLSLSGVEEAIVKFAAAKGDSSLKAAMASQSVVQLGLLMTLPM 1554

Query: 185  VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 244
            VMEIGLE+GFRTAL D +IMQLQLA VFFTF LGTK H+YGRTILHGGSKYRATGRGFVV
Sbjct: 1555 VMEIGLERGFRTALSDLIIMQLQLAPVFFTFSLGTKVHYYGRTILHGGSKYRATGRGFVV 1614

Query: 245  FHARFADNYRQYSRSHFVKGLELFILLLVYQIYGESYRSSKLYLFITFSMWFLVASWLFA 304
             H +FA+NYR YSRSHFVKG+EL +LL+ Y+IYG++   S  Y  +  S WFLV SWLFA
Sbjct: 1615 KHEKFAENYRMYSRSHFVKGMELMVLLICYRIYGKAAEDSVGYALVMGSTWFLVGSWLFA 1674

Query: 305  PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDAEQEHLKSTTIRGRML 364
            PF FNPSGF+WQK VDDW DW +W+ +RGGIG+  +KSWESWW+ EQEHL  +   G+  
Sbjct: 1675 PFFFNPSGFEWQKIVDDWDDWNKWISSRGGIGVPANKSWESWWEEEQEHLLHSGFFGKFW 1734

Query: 365  EIILALRFLIYQYGIVYHLDISHRVK-----SLLVYGLSWVVMLIALVVLKLVSMGRRKF 424
            EI L+LR+ IYQYGIVY L+++   +     S++VYGLSW+V++  ++VLK+VSMGR+KF
Sbjct: 1735 EIFLSLRYFIYQYGIVYQLNLTKESRMGKQHSIIVYGLSWLVIVAVMIVLKIVSMGRKKF 1794

Query: 425  GTDFQLMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAILAFLPTGWAILLIGQACRP 484
              DFQLMFR+LK  LF+G + ++ +LF    LTV D+  ++LAFLPTGWA+L I Q  RP
Sbjct: 1795 SADFQLMFRLLKLFLFIGSVVIVGMLFHFLKLTVGDIMQSLLAFLPTGWALLQISQVARP 1854

Query: 485  MMKGIGFWESIKELARAYEYIMGLIIFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISM 544
            +MK +G W S+K LAR YEYIMG++IFMP+ +L+WFPFVSEFQTRLLFNQAFSRGLQI  
Sbjct: 1855 LMKTVGMWGSVKALARGYEYIMGVVIFMPVTVLAWFPFVSEFQTRLLFNQAFSRGLQIQR 1914

Query: 545  ILSGRKE 547
            IL+G K+
Sbjct: 1915 ILAGGKK 1921

BLAST of Spg007811 vs. ExPASy TrEMBL
Match: A0A6J1JJ30 (1,3-beta-glucan synthase OS=Cucurbita maxima OX=3661 GN=LOC111486272 PE=3 SV=1)

HSP 1 Score: 1041.6 bits (2692), Expect = 1.2e-300
Identity = 526/557 (94.43%), Postives = 544/557 (97.67%), Query Frame = 0

Query: 5    QKLTCHFYRVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGGLVTHH 64
            Q++  +  RVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGG VTHH
Sbjct: 1372 QRILANPLRVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGGFVTHH 1431

Query: 65   EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYF 124
            EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDF+RMLSFYFTTVGFYF
Sbjct: 1432 EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFFRMLSFYFTTVGFYF 1491

Query: 125  SSMVTVLTVYVFLYGRLYMVMSGVEREILENPSIHQTKALEEALATQSVFQLGLLLVLPM 184
            SSMVTVLTVYVFLYGRLYMVMSGVEREIL NPSI QTKALEEALATQSVFQLGLLLVLPM
Sbjct: 1492 SSMVTVLTVYVFLYGRLYMVMSGVEREILGNPSIRQTKALEEALATQSVFQLGLLLVLPM 1551

Query: 185  VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 244
            VMEIGLEKGFRTALGDF+IMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV
Sbjct: 1552 VMEIGLEKGFRTALGDFIIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 1611

Query: 245  FHARFADNYRQYSRSHFVKGLELFILLLVYQIYGESYRSSKLYLFITFSMWFLVASWLFA 304
            FHA+FADNYRQYSRSHFVKGLEL ILLLVYQIYG+SYRSSKLYLFITFSMWFLVASWLFA
Sbjct: 1612 FHAKFADNYRQYSRSHFVKGLELLILLLVYQIYGQSYRSSKLYLFITFSMWFLVASWLFA 1671

Query: 305  PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDAEQEHLKSTTIRGRML 364
            PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWD EQEHLKSTTIRGR+L
Sbjct: 1672 PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDGEQEHLKSTTIRGRVL 1731

Query: 365  EIILALRFLIYQYGIVYHLDISHRVKSLLVYGLSWVVMLIALVVLKLVSMGRRKFGTDFQ 424
            EIILALRFLI+QYGIVYHLDISHR+KSLLVYGLSWVVM+I LVVLKLVSMGRRKFGTDFQ
Sbjct: 1732 EIILALRFLIFQYGIVYHLDISHRIKSLLVYGLSWVVMVIVLVVLKLVSMGRRKFGTDFQ 1791

Query: 425  LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAILAFLPTGWAILLIGQACRPMMKGI 484
            LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAI+AFLPTGWAI+LIGQACRPMMKGI
Sbjct: 1792 LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAIIAFLPTGWAIVLIGQACRPMMKGI 1851

Query: 485  GFWESIKELARAYEYIMGLIIFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMILSGR 544
            G WESIKELARAYEYIMGLIIFMP A+LSWFPFVSEFQTRLLFNQAFSRGLQISMIL+G+
Sbjct: 1852 GLWESIKELARAYEYIMGLIIFMPTAVLSWFPFVSEFQTRLLFNQAFSRGLQISMILAGK 1911

Query: 545  KETPSMILAGKKETPST 562
            KETPSM++ GKKETPST
Sbjct: 1912 KETPSMVITGKKETPST 1928

BLAST of Spg007811 vs. ExPASy TrEMBL
Match: A0A6J1EVT3 (1,3-beta-glucan synthase OS=Cucurbita moschata OX=3662 GN=LOC111437035 PE=3 SV=1)

HSP 1 Score: 1038.9 bits (2685), Expect = 7.8e-300
Identity = 526/556 (94.60%), Postives = 543/556 (97.66%), Query Frame = 0

Query: 5    QKLTCHFYRVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGGLVTHH 64
            Q++  +  RVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGG VTHH
Sbjct: 1372 QRILANPLRVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGGFVTHH 1431

Query: 65   EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYF 124
            EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDF+RMLSFYFTTVGFYF
Sbjct: 1432 EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFFRMLSFYFTTVGFYF 1491

Query: 125  SSMVTVLTVYVFLYGRLYMVMSGVEREILENPSIHQTKALEEALATQSVFQLGLLLVLPM 184
            SSMVTVLTVYVFLYGRLYMVMSGVEREIL NPSI QTKALEEALATQSVFQLGLLLVLPM
Sbjct: 1492 SSMVTVLTVYVFLYGRLYMVMSGVEREILGNPSIRQTKALEEALATQSVFQLGLLLVLPM 1551

Query: 185  VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 244
            VMEIGLEKGFRTALGDF+IMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV
Sbjct: 1552 VMEIGLEKGFRTALGDFIIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 1611

Query: 245  FHARFADNYRQYSRSHFVKGLELFILLLVYQIYGESYRSSKLYLFITFSMWFLVASWLFA 304
            FHA+FADNYRQYSRSHFVKGLEL ILLLVYQIYG+SYRSSKLYLFITFSMWFLVASWLFA
Sbjct: 1612 FHAKFADNYRQYSRSHFVKGLELLILLLVYQIYGQSYRSSKLYLFITFSMWFLVASWLFA 1671

Query: 305  PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDAEQEHLKSTTIRGRML 364
            PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWD EQEHLKSTTIRGR+L
Sbjct: 1672 PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDGEQEHLKSTTIRGRVL 1731

Query: 365  EIILALRFLIYQYGIVYHLDISHRVKSLLVYGLSWVVMLIALVVLKLVSMGRRKFGTDFQ 424
            EIILALRFLI+QYGIVYHLDISHR+KSLLVYGLSWVVM+IALVVLKLVSMGRRKFGTDFQ
Sbjct: 1732 EIILALRFLIFQYGIVYHLDISHRIKSLLVYGLSWVVMVIALVVLKLVSMGRRKFGTDFQ 1791

Query: 425  LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAILAFLPTGWAILLIGQACRPMMKGI 484
            LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAI+AFLPTGWAI+LIGQACRPMMKGI
Sbjct: 1792 LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAIIAFLPTGWAIVLIGQACRPMMKGI 1851

Query: 485  GFWESIKELARAYEYIMGLIIFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMILSGR 544
            GFWESIKELARAYEYIMGL+I MP AILSWFPFVSEFQTRLLFNQAFSRGLQISMIL+G+
Sbjct: 1852 GFWESIKELARAYEYIMGLVILMPTAILSWFPFVSEFQTRLLFNQAFSRGLQISMILAGK 1911

Query: 545  KETPSMILAGKKETPS 561
            KETPSMI+  KKETPS
Sbjct: 1912 KETPSMIITEKKETPS 1927

BLAST of Spg007811 vs. ExPASy TrEMBL
Match: A0A6J1D463 (1,3-beta-glucan synthase OS=Momordica charantia OX=3673 GN=LOC111016827 PE=3 SV=1)

HSP 1 Score: 1036.6 bits (2679), Expect = 3.9e-299
Identity = 529/545 (97.06%), Postives = 537/545 (98.53%), Query Frame = 0

Query: 5    QKLTCHFYRVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGGLVTHH 64
            Q++  +  RVRFHYGHPDIFDRIFHITRGGISKASRV+NLSEDIFAGYNSTLRGG VTHH
Sbjct: 1377 QRILANPLRVRFHYGHPDIFDRIFHITRGGISKASRVINLSEDIFAGYNSTLRGGFVTHH 1436

Query: 65   EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYF 124
            EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFYRM+SFYFTTVGFYF
Sbjct: 1437 EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFYRMMSFYFTTVGFYF 1496

Query: 125  SSMVTVLTVYVFLYGRLYMVMSGVEREILENPSIHQTKALEEALATQSVFQLGLLLVLPM 184
            SSMVTVLTVYVFLYGRLYMVMSGVEREILENPSI QTKALEEALATQSVFQLGLLLVLPM
Sbjct: 1497 SSMVTVLTVYVFLYGRLYMVMSGVEREILENPSIRQTKALEEALATQSVFQLGLLLVLPM 1556

Query: 185  VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 244
            VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV
Sbjct: 1557 VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 1616

Query: 245  FHARFADNYRQYSRSHFVKGLELFILLLVYQIYGESYRSSKLYLFITFSMWFLVASWLFA 304
            FHA+FADNYRQYSRSHFVKGLELFILLLVYQIYGESYRSS+LYLFITFSMWFLVASWLFA
Sbjct: 1617 FHAKFADNYRQYSRSHFVKGLELFILLLVYQIYGESYRSSRLYLFITFSMWFLVASWLFA 1676

Query: 305  PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDAEQEHLKSTTIRGRML 364
            PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWD EQEHLKSTTIRGR+L
Sbjct: 1677 PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDGEQEHLKSTTIRGRVL 1736

Query: 365  EIILALRFLIYQYGIVYHLDISHRVKSLLVYGLSWVVMLIALVVLKLVSMGRRKFGTDFQ 424
            EIILALRFLIYQYGIVYHLDISHRVKSLLVYGLSWVVMLIALVVLKLVSMGRRKFGTDFQ
Sbjct: 1737 EIILALRFLIYQYGIVYHLDISHRVKSLLVYGLSWVVMLIALVVLKLVSMGRRKFGTDFQ 1796

Query: 425  LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAILAFLPTGWAILLIGQACRPMMKGI 484
            LMFRILKALLFLGFMSVMTVLFVV GLTVSDLFAAILAFLPTGWAILLIGQACRPMMKGI
Sbjct: 1797 LMFRILKALLFLGFMSVMTVLFVVGGLTVSDLFAAILAFLPTGWAILLIGQACRPMMKGI 1856

Query: 485  GFWESIKELARAYEYIMGLIIFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMILSGR 544
            GFWESIKELARAYEYIMGLIIFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMILSGR
Sbjct: 1857 GFWESIKELARAYEYIMGLIIFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMILSGR 1916

Query: 545  KETPS 550
            KETPS
Sbjct: 1917 KETPS 1921

BLAST of Spg007811 vs. ExPASy TrEMBL
Match: A0A1S3BG55 (1,3-beta-glucan synthase OS=Cucumis melo OX=3656 GN=LOC103489654 PE=3 SV=1)

HSP 1 Score: 1024.6 bits (2648), Expect = 1.5e-295
Identity = 520/557 (93.36%), Postives = 537/557 (96.41%), Query Frame = 0

Query: 5    QKLTCHFYRVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGGLVTHH 64
            Q++  +  RVRFHYGHPDIFDRIFHITRGGISKASRV+NLSEDIFAGYNSTLRGG VTHH
Sbjct: 1376 QRILANPLRVRFHYGHPDIFDRIFHITRGGISKASRVINLSEDIFAGYNSTLRGGFVTHH 1435

Query: 65   EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYF 124
            EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYF
Sbjct: 1436 EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYF 1495

Query: 125  SSMVTVLTVYVFLYGRLYMVMSGVEREILENPSIHQTKALEEALATQSVFQLGLLLVLPM 184
            SSMVTVLTVY+FLYGRLYMVMSGVEREIL++PS+HQTKALEEALATQSVFQLGLLLVLPM
Sbjct: 1496 SSMVTVLTVYLFLYGRLYMVMSGVEREILDSPSVHQTKALEEALATQSVFQLGLLLVLPM 1555

Query: 185  VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 244
            VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV
Sbjct: 1556 VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 1615

Query: 245  FHARFADNYRQYSRSHFVKGLELFILLLVYQIYGESYRSSKLYLFITFSMWFLVASWLFA 304
            FHA+FADNYRQYSRSHFVKGLELFILLLVYQIYG SYRSSKLYLFITFSMWFLVASWLFA
Sbjct: 1616 FHAKFADNYRQYSRSHFVKGLELFILLLVYQIYGSSYRSSKLYLFITFSMWFLVASWLFA 1675

Query: 305  PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDAEQEHLKSTTIRGRML 364
            PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWD EQEHLKSTTIRGR+L
Sbjct: 1676 PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDGEQEHLKSTTIRGRVL 1735

Query: 365  EIILALRFLIYQYGIVYHLDISHRVKSLLVYGLSWVVMLIALVVLKLVSMGRRKFGTDFQ 424
            EI+ +LRFL+YQYGIVYHLDISH +KS  VYGLSWVVM IALVVLKLVSMGRRKFGTDFQ
Sbjct: 1736 EIVFSLRFLLYQYGIVYHLDISHNIKSFWVYGLSWVVMFIALVVLKLVSMGRRKFGTDFQ 1795

Query: 425  LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAILAFLPTGWAILLIGQACRPMMKGI 484
            LMFRILKALLFLGFMSVMTVLFVV GLTVSDLFAAILAFLPTGWAILLIGQACRPMMKGI
Sbjct: 1796 LMFRILKALLFLGFMSVMTVLFVVWGLTVSDLFAAILAFLPTGWAILLIGQACRPMMKGI 1855

Query: 485  GFWESIKELARAYEYIMGLIIFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMILSGR 544
            GFWESIKELAR YEYIMGL+IFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMIL+GR
Sbjct: 1856 GFWESIKELARGYEYIMGLVIFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMILAGR 1915

Query: 545  KETPSMILAGKKETPST 562
            KETPS I+     TPST
Sbjct: 1916 KETPSTIM--PSTTPST 1930

BLAST of Spg007811 vs. ExPASy TrEMBL
Match: A0A0A0K413 (1,3-beta-glucan synthase OS=Cucumis sativus OX=3659 GN=Csa_7G236800 PE=3 SV=1)

HSP 1 Score: 1019.6 bits (2635), Expect = 4.9e-294
Identity = 519/557 (93.18%), Postives = 535/557 (96.05%), Query Frame = 0

Query: 5    QKLTCHFYRVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGGLVTHH 64
            Q++  +  RVRFHYGHPDIFDRIFHITRGGISKASRV+NLSEDIFAGYNSTLRGG VTHH
Sbjct: 1373 QRILANPLRVRFHYGHPDIFDRIFHITRGGISKASRVINLSEDIFAGYNSTLRGGFVTHH 1432

Query: 65   EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYF 124
            EYIQVGKGRDVGMNQISLFEAKVANGNGEQTL RDVYRLGRRFDFYRMLSFYFTTVGFYF
Sbjct: 1433 EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLCRDVYRLGRRFDFYRMLSFYFTTVGFYF 1492

Query: 125  SSMVTVLTVYVFLYGRLYMVMSGVEREILENPSIHQTKALEEALATQSVFQLGLLLVLPM 184
            SSMVTVLTVY+FLYGRLYMVMSGVEREIL++PS+ QTKALEEALATQSVFQLGLLLVLPM
Sbjct: 1493 SSMVTVLTVYLFLYGRLYMVMSGVEREILDSPSVRQTKALEEALATQSVFQLGLLLVLPM 1552

Query: 185  VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 244
            VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYR+TGRGFVV
Sbjct: 1553 VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRSTGRGFVV 1612

Query: 245  FHARFADNYRQYSRSHFVKGLELFILLLVYQIYGESYRSSKLYLFITFSMWFLVASWLFA 304
            FHA+FADNYRQYSRSHFVKGLELFILLLVYQIYG SYRSSKLYLFITFSMWFLVASWLFA
Sbjct: 1613 FHAKFADNYRQYSRSHFVKGLELFILLLVYQIYGSSYRSSKLYLFITFSMWFLVASWLFA 1672

Query: 305  PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDAEQEHLKSTTIRGRML 364
            PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWD EQEHLKSTTIRGR+L
Sbjct: 1673 PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDGEQEHLKSTTIRGRVL 1732

Query: 365  EIILALRFLIYQYGIVYHLDISHRVKSLLVYGLSWVVMLIALVVLKLVSMGRRKFGTDFQ 424
            EII +LRFL+YQYGIVYHLDISH +KS  VYGLSWVVMLIALVVLKLVSMGRRKFGTDFQ
Sbjct: 1733 EIIFSLRFLLYQYGIVYHLDISHNIKSFWVYGLSWVVMLIALVVLKLVSMGRRKFGTDFQ 1792

Query: 425  LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAILAFLPTGWAILLIGQACRPMMKGI 484
            LMFRILKALLFLGFMSVMTVLFVV GLTVSDLFAAILAFLPTGWAILLIGQACRPMMKGI
Sbjct: 1793 LMFRILKALLFLGFMSVMTVLFVVWGLTVSDLFAAILAFLPTGWAILLIGQACRPMMKGI 1852

Query: 485  GFWESIKELARAYEYIMGLIIFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMILSGR 544
            GFWESIKELAR YEYIMGL+IFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMILSGR
Sbjct: 1853 GFWESIKELARGYEYIMGLVIFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMILSGR 1912

Query: 545  KETPSMILAGKKETPST 562
            KETPS  +     TPST
Sbjct: 1913 KETPSTTM--PSTTPST 1927

BLAST of Spg007811 vs. TAIR 10
Match: AT1G06490.1 (glucan synthase-like 7 )

HSP 1 Score: 931.8 bits (2407), Expect = 2.6e-271
Identity = 460/557 (82.59%), Postives = 513/557 (92.10%), Query Frame = 0

Query: 5    QKLTCHFYRVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGGLVTHH 64
            Q++  +  RVRFHYGHPDIFDRIFHITRGGISKAS+++NLSEDIFAGYNSTLRGG VTHH
Sbjct: 1385 QRVLANPLRVRFHYGHPDIFDRIFHITRGGISKASKIINLSEDIFAGYNSTLRGGYVTHH 1444

Query: 65   EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYF 124
            EYIQ GKGRDVGMNQIS FEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYF
Sbjct: 1445 EYIQAGKGRDVGMNQISFFEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYF 1504

Query: 125  SSMVTVLTVYVFLYGRLYMVMSGVEREILENPSIHQTKALEEALATQSVFQLGLLLVLPM 184
            SSM+TVLTVYVFLYGRLY+V+SG+E+ IL++ S+H++ ALE+ALA QSVFQLG L+VLPM
Sbjct: 1505 SSMITVLTVYVFLYGRLYLVLSGLEKNILQSASVHESNALEQALAAQSVFQLGFLMVLPM 1564

Query: 185  VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 244
            VMEIGLEKGFRTALGDF+IMQLQLASVFFTFQLGTKAH++GRTILHGGSKYRATGRGFVV
Sbjct: 1565 VMEIGLEKGFRTALGDFIIMQLQLASVFFTFQLGTKAHYFGRTILHGGSKYRATGRGFVV 1624

Query: 245  FHARFADNYRQYSRSHFVKGLELFILLLVYQIYGESYRSSKLYLFITFSMWFLVASWLFA 304
            FHA+FA+NYR YSRSHFVKGLEL ILL+VYQ+YG SYRSS  Y++ITFSMWFLV SWLFA
Sbjct: 1625 FHAKFAENYRLYSRSHFVKGLELVILLVVYQVYGTSYRSSSTYMYITFSMWFLVTSWLFA 1684

Query: 305  PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDAEQEHLKSTTIRGRML 364
            PF+FNPSGF+WQKTVDDWTDWKRWMGNRGGIGI  DKSWESWWD EQEHLK T +RGR+L
Sbjct: 1685 PFIFNPSGFEWQKTVDDWTDWKRWMGNRGGIGIVLDKSWESWWDIEQEHLKHTNLRGRVL 1744

Query: 365  EIILALRFLIYQYGIVYHLDISHRVKSLLVYGLSWVVMLIALVVLKLVSMGRRKFGTDFQ 424
            EI+LALRFL+YQYGIVYHL+I+ R  + LVYGLSW ++L  L+VLK+VSMGRRKFGTDFQ
Sbjct: 1745 EILLALRFLLYQYGIVYHLNIARRHTTFLVYGLSWAILLSVLLVLKMVSMGRRKFGTDFQ 1804

Query: 425  LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAILAFLPTGWAILLIGQACRPMMKGI 484
            +MFRILKALLFLGF+SVMTVLFVVCGLT+SDLFA+ILAFLPTGWAILLIGQA R + KG+
Sbjct: 1805 VMFRILKALLFLGFLSVMTVLFVVCGLTISDLFASILAFLPTGWAILLIGQALRSVFKGL 1864

Query: 485  GFWESIKELARAYEYIMGLIIFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMILSGR 544
            GFW+S+KEL RAYEYIMGL+IF PIA+LSWFPFVSEFQTRLLFNQAFSRGLQISMIL+G+
Sbjct: 1865 GFWDSVKELGRAYEYIMGLVIFTPIAVLSWFPFVSEFQTRLLFNQAFSRGLQISMILAGK 1924

Query: 545  KETPSMILAGKKETPST 562
            K+         KETPST
Sbjct: 1925 KD---------KETPST 1932

BLAST of Spg007811 vs. TAIR 10
Match: AT3G59100.1 (glucan synthase-like 11 )

HSP 1 Score: 877.5 bits (2266), Expect = 5.8e-255
Identity = 435/542 (80.26%), Postives = 492/542 (90.77%), Query Frame = 0

Query: 5    QKLTCHFYRVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGGLVTHH 64
            Q++  +  RVRFHYGHPDIFDRIFHITRGG+SKAS+V+NLSEDIF G+NSTLRGG VTHH
Sbjct: 1373 QRILANPLRVRFHYGHPDIFDRIFHITRGGVSKASKVINLSEDIFGGFNSTLRGGYVTHH 1432

Query: 65   EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYF 124
            EYIQVGKGRDVG+N IS+FEAKVANGNGEQTLSRDVYRLG RFDFYRMLSFYFTT+GFYF
Sbjct: 1433 EYIQVGKGRDVGLNPISIFEAKVANGNGEQTLSRDVYRLGHRFDFYRMLSFYFTTIGFYF 1492

Query: 125  SSMVTVLTVYVFLYGRLYMVMSGVEREILENPSIHQTKALEEALATQSVFQLGLLLVLPM 184
            SSM+TVLTVY FLYGR+YMVMSG+E+EIL   S +Q +ALE+ALATQS+FQLG L+VLPM
Sbjct: 1493 SSMLTVLTVYAFLYGRMYMVMSGLEKEILRLASPNQLEALEQALATQSIFQLGFLMVLPM 1552

Query: 185  VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 244
            VMEIGLE GFR+A+ DF IMQLQLASVFFTFQLGTK+H+YGRTILHGGSKYR TGRGFVV
Sbjct: 1553 VMEIGLEHGFRSAIVDFFIMQLQLASVFFTFQLGTKSHYYGRTILHGGSKYRPTGRGFVV 1612

Query: 245  FHARFADNYRQYSRSHFVKGLELFILLLVYQIYGESYRSSKLYLFITFSMWFLVASWLFA 304
            FHA+FA+NYR YSRSHFVKGLEL +LL+VYQIYG SYRSS LYL+IT SMWF+V SWLFA
Sbjct: 1613 FHAKFAENYRLYSRSHFVKGLELLLLLVVYQIYGHSYRSSNLYLYITVSMWFMVGSWLFA 1672

Query: 305  PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDAEQEHLKSTTIRGRML 364
            PF+FNPSGF+WQKTVDDWTDWKRW+G+RGGIGI  +KSWESWW+ EQEHLK T+IRGR+L
Sbjct: 1673 PFIFNPSGFEWQKTVDDWTDWKRWLGDRGGIGIPVEKSWESWWNVEQEHLKHTSIRGRIL 1732

Query: 365  EIILALRFLIYQYGIVYHLDISHRVKSLLVYGLSWVVMLIALVVLKLVSMGRRKFGTDFQ 424
            EI LALRF IYQYGIVY L+IS R KS LVYGLSWVV+L +L+VLK+VSMGRR+FGTDFQ
Sbjct: 1733 EITLALRFFIYQYGIVYQLNISQRSKSFLVYGLSWVVLLTSLLVLKMVSMGRRRFGTDFQ 1792

Query: 425  LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAILAFLPTGWAILLIGQACRPMMKGI 484
            LMFRILKALLFLGF+SVMT+LFVV  LT++DL A++LAFLPTGWAILLIGQ  R  +K +
Sbjct: 1793 LMFRILKALLFLGFLSVMTILFVVFKLTLTDLSASVLAFLPTGWAILLIGQVLRSPIKAL 1852

Query: 485  GFWESIKELARAYEYIMGLIIFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMILSGR 544
            G W+S+KEL RAYE IMGL+IF PIA+LSWFP VSEFQ RLLFNQAFSRGLQISMIL+GR
Sbjct: 1853 GVWDSVKELGRAYENIMGLVIFAPIAVLSWFPIVSEFQARLLFNQAFSRGLQISMILAGR 1912

Query: 545  KE 547
            K+
Sbjct: 1913 KD 1914

BLAST of Spg007811 vs. TAIR 10
Match: AT5G13000.1 (glucan synthase-like 12 )

HSP 1 Score: 770.4 bits (1988), Expect = 1.0e-222
Identity = 362/545 (66.42%), Postives = 457/545 (83.85%), Query Frame = 0

Query: 5    QKLTCHFYRVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGGLVTHH 64
            Q+L  +  RVRFHYGHPD+FDR+FH+TRGG+SKAS+V+NLSEDIFAG+NSTLR G VTHH
Sbjct: 1406 QRLLANPLRVRFHYGHPDVFDRLFHLTRGGVSKASKVINLSEDIFAGFNSTLREGNVTHH 1465

Query: 65   EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYF 124
            EYIQVGKGRDVG+NQIS+FEAK+ANGNGEQTLSRD+YRLG RFDF+RM+S YFTTVGFYF
Sbjct: 1466 EYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRDIYRLGHRFDFFRMMSCYFTTVGFYF 1525

Query: 125  SSMVTVLTVYVFLYGRLYMVMSGVEREILENPSIHQTKALEEALATQSVFQLGLLLVLPM 184
            S+++TVLTVY+FLYGRLY+V+SG+E+ +     I     L+ ALA+QS  Q+G L+ LPM
Sbjct: 1526 STLITVLTVYIFLYGRLYLVLSGLEQGLSTQKGIRDNTPLQIALASQSFVQIGFLMALPM 1585

Query: 185  VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 244
            +MEIGLE+GFRTAL +FV+MQLQLA VFFTF LGTK H+YGRT+LHGG+KYR+TGRGFVV
Sbjct: 1586 LMEIGLERGFRTALSEFVLMQLQLAPVFFTFSLGTKTHYYGRTLLHGGAKYRSTGRGFVV 1645

Query: 245  FHARFADNYRQYSRSHFVKGLELFILLLVYQIYGESYRSSKLYLFITFSMWFLVASWLFA 304
            FHA+FADNYR YSRSHFVKGLE+ +LL+VYQI+G +YR    YL IT SMWF+V +WLFA
Sbjct: 1646 FHAKFADNYRLYSRSHFVKGLEMMLLLVVYQIFGSAYRGVLAYLLITISMWFMVGTWLFA 1705

Query: 305  PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDAEQEHLKSTTIRGRML 364
            PF+FNPSGF+WQK VDDWTDW +W+ N GGIG+  +KSWESWW+ EQEHL+ +  RG ++
Sbjct: 1706 PFLFNPSGFEWQKIVDDWTDWNKWINNIGGIGVPAEKSWESWWEEEQEHLRYSGKRGIVV 1765

Query: 365  EIILALRFLIYQYGIVYHLDISHRVKSLLVYGLSWVVMLIALVVLKLVSMGRRKFGTDFQ 424
            EI+LALRF IYQYG+VYHL I+ + K+ LVYG+SW+V+ + L V+K VS+GRR+F   FQ
Sbjct: 1766 EILLALRFFIYQYGLVYHLTITEKTKNFLVYGVSWLVIFLILFVMKTVSVGRRRFSASFQ 1825

Query: 425  LMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAILAFLPTGWAILLIGQACRPMMKGI 484
            LMFR++K L+F+ F++++ +L  +  +T+ D+   ILAF+PTGW +LLI QAC+P++   
Sbjct: 1826 LMFRLIKGLIFMTFIAIIVILITLAHMTIQDIIVCILAFMPTGWGMLLIAQACKPVVHRA 1885

Query: 485  GFWESIKELARAYEYIMGLIIFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMILSGR 544
            GFW S++ LAR YE +MGL++F P+A L+WFPFVSEFQTR+LFNQAFSRGLQIS IL G 
Sbjct: 1886 GFWGSVRTLARGYEIVMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGGH 1945

Query: 545  KETPS 550
            ++  S
Sbjct: 1946 RKDRS 1950

BLAST of Spg007811 vs. TAIR 10
Match: AT1G05570.1 (callose synthase 1 )

HSP 1 Score: 748.4 bits (1931), Expect = 4.1e-216
Identity = 359/546 (65.75%), Postives = 451/546 (82.60%), Query Frame = 0

Query: 5    QKLTCHFYRVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGGLVTHH 64
            Q++     +VRFHYGHPDIFDR+FH+TRGGI KAS+V+NLSEDIFAG+NSTLR G VTHH
Sbjct: 1400 QRVLASPLKVRFHYGHPDIFDRLFHLTRGGICKASKVINLSEDIFAGFNSTLREGNVTHH 1459

Query: 65   EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYF 124
            EYIQVGKGRDVG+NQIS+FEAK+ANGNGEQTLSRD+YRLG RFDF+RMLS YFTT+GFYF
Sbjct: 1460 EYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRDLYRLGHRFDFFRMLSCYFTTIGFYF 1519

Query: 125  SSMVTVLTVYVFLYGRLYMVMSGVEREILENPSIHQTKALEEALATQSVFQLGLLLVLPM 184
            S+M+TVLTVYVFLYGRLY+V+SG+E  +    +    K LE ALA+QS  Q+G L+ LPM
Sbjct: 1520 STMLTVLTVYVFLYGRLYLVLSGLEEGLSSQRAFRNNKPLEAALASQSFVQIGFLMALPM 1579

Query: 185  VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 244
            +MEIGLE+GF  AL +FV+MQLQLASVFFTFQLGTK H+YGRT+ HGG++YR TGRGFVV
Sbjct: 1580 MMEIGLERGFHNALIEFVLMQLQLASVFFTFQLGTKTHYYGRTLFHGGAEYRGTGRGFVV 1639

Query: 245  FHARFADNYRQYSRSHFVKGLELFILLLVYQIYGESYRSSKLYLFITFSMWFLVASWLFA 304
            FHA+FA+NYR YSRSHFVKG+EL ILLLVYQI+G+SYR    Y+ IT S+WF+V +WLFA
Sbjct: 1640 FHAKFAENYRFYSRSHFVKGIELMILLLVYQIFGQSYRGVVTYILITVSIWFMVVTWLFA 1699

Query: 305  PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDAEQEHLKSTTIRGRML 364
            PF+FNPSGF+WQK VDDWTDW +W+ NRGGIG+  +KSWESWW+ E EHL+ + +RG  L
Sbjct: 1700 PFLFNPSGFEWQKIVDDWTDWNKWIYNRGGIGVPPEKSWESWWEKELEHLRHSGVRGITL 1759

Query: 365  EIILALRFLIYQYGIVYHLD-ISHRVKSLLVYGLSWVVMLIALVVLKLVSMGRRKFGTDF 424
            EI LALRF I+QYG+VYHL     + +S  VYG SW V+L  L+++K + +GRR+F T+F
Sbjct: 1760 EIFLALRFFIFQYGLVYHLSTFKGKNQSFWVYGASWFVILFILLIVKGLGVGRRRFSTNF 1819

Query: 425  QLMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAILAFLPTGWAILLIGQACRPMMKG 484
            QL+FRI+K L+FL F++++     +  +T+ DLF  +LAF+PTGW +LLI QAC+P+++ 
Sbjct: 1820 QLLFRIIKGLVFLTFVAILITFLALPLITIKDLFICMLAFMPTGWGMLLIAQACKPLIQQ 1879

Query: 485  IGFWESIKELARAYEYIMGLIIFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMILSG 544
            +G W S++ LAR YE +MGL++F P+A L+WFPFVSEFQTR+LFNQAFSRGLQIS IL G
Sbjct: 1880 LGIWSSVRTLARGYEIVMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGG 1939

Query: 545  RKETPS 550
            +++  S
Sbjct: 1940 QRKDRS 1945

BLAST of Spg007811 vs. TAIR 10
Match: AT1G05570.2 (callose synthase 1 )

HSP 1 Score: 748.4 bits (1931), Expect = 4.1e-216
Identity = 359/546 (65.75%), Postives = 451/546 (82.60%), Query Frame = 0

Query: 5    QKLTCHFYRVRFHYGHPDIFDRIFHITRGGISKASRVVNLSEDIFAGYNSTLRGGLVTHH 64
            Q++     +VRFHYGHPDIFDR+FH+TRGGI KAS+V+NLSEDIFAG+NSTLR G VTHH
Sbjct: 1359 QRVLASPLKVRFHYGHPDIFDRLFHLTRGGICKASKVINLSEDIFAGFNSTLREGNVTHH 1418

Query: 65   EYIQVGKGRDVGMNQISLFEAKVANGNGEQTLSRDVYRLGRRFDFYRMLSFYFTTVGFYF 124
            EYIQVGKGRDVG+NQIS+FEAK+ANGNGEQTLSRD+YRLG RFDF+RMLS YFTT+GFYF
Sbjct: 1419 EYIQVGKGRDVGLNQISMFEAKIANGNGEQTLSRDLYRLGHRFDFFRMLSCYFTTIGFYF 1478

Query: 125  SSMVTVLTVYVFLYGRLYMVMSGVEREILENPSIHQTKALEEALATQSVFQLGLLLVLPM 184
            S+M+TVLTVYVFLYGRLY+V+SG+E  +    +    K LE ALA+QS  Q+G L+ LPM
Sbjct: 1479 STMLTVLTVYVFLYGRLYLVLSGLEEGLSSQRAFRNNKPLEAALASQSFVQIGFLMALPM 1538

Query: 185  VMEIGLEKGFRTALGDFVIMQLQLASVFFTFQLGTKAHFYGRTILHGGSKYRATGRGFVV 244
            +MEIGLE+GF  AL +FV+MQLQLASVFFTFQLGTK H+YGRT+ HGG++YR TGRGFVV
Sbjct: 1539 MMEIGLERGFHNALIEFVLMQLQLASVFFTFQLGTKTHYYGRTLFHGGAEYRGTGRGFVV 1598

Query: 245  FHARFADNYRQYSRSHFVKGLELFILLLVYQIYGESYRSSKLYLFITFSMWFLVASWLFA 304
            FHA+FA+NYR YSRSHFVKG+EL ILLLVYQI+G+SYR    Y+ IT S+WF+V +WLFA
Sbjct: 1599 FHAKFAENYRFYSRSHFVKGIELMILLLVYQIFGQSYRGVVTYILITVSIWFMVVTWLFA 1658

Query: 305  PFVFNPSGFDWQKTVDDWTDWKRWMGNRGGIGISHDKSWESWWDAEQEHLKSTTIRGRML 364
            PF+FNPSGF+WQK VDDWTDW +W+ NRGGIG+  +KSWESWW+ E EHL+ + +RG  L
Sbjct: 1659 PFLFNPSGFEWQKIVDDWTDWNKWIYNRGGIGVPPEKSWESWWEKELEHLRHSGVRGITL 1718

Query: 365  EIILALRFLIYQYGIVYHLD-ISHRVKSLLVYGLSWVVMLIALVVLKLVSMGRRKFGTDF 424
            EI LALRF I+QYG+VYHL     + +S  VYG SW V+L  L+++K + +GRR+F T+F
Sbjct: 1719 EIFLALRFFIFQYGLVYHLSTFKGKNQSFWVYGASWFVILFILLIVKGLGVGRRRFSTNF 1778

Query: 425  QLMFRILKALLFLGFMSVMTVLFVVCGLTVSDLFAAILAFLPTGWAILLIGQACRPMMKG 484
            QL+FRI+K L+FL F++++     +  +T+ DLF  +LAF+PTGW +LLI QAC+P+++ 
Sbjct: 1779 QLLFRIIKGLVFLTFVAILITFLALPLITIKDLFICMLAFMPTGWGMLLIAQACKPLIQQ 1838

Query: 485  IGFWESIKELARAYEYIMGLIIFMPIAILSWFPFVSEFQTRLLFNQAFSRGLQISMILSG 544
            +G W S++ LAR YE +MGL++F P+A L+WFPFVSEFQTR+LFNQAFSRGLQIS IL G
Sbjct: 1839 LGIWSSVRTLARGYEIVMGLLLFTPVAFLAWFPFVSEFQTRMLFNQAFSRGLQISRILGG 1898

Query: 545  RKETPS 550
            +++  S
Sbjct: 1899 QRKDRS 1904

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023530416.11.0e-30195.15callose synthase 7 [Cucurbita pepo subsp. pepo][more]
XP_022989096.12.5e-30094.43callose synthase 7-like [Cucurbita maxima][more]
KAG6589303.13.3e-30094.78Callose synthase 7, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022930628.11.6e-29994.60callose synthase 7-like [Cucurbita moschata][more]
XP_022148041.18.0e-29997.06callose synthase 7 isoform X1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9SHJ33.7e-27082.59Callose synthase 7 OS=Arabidopsis thaliana OX=3702 GN=CALS7 PE=3 SV=3[more]
Q9LYS68.2e-25480.26Putative callose synthase 6 OS=Arabidopsis thaliana OX=3702 GN=CALS6 PE=3 SV=2[more]
Q9LXT91.4e-22166.42Callose synthase 3 OS=Arabidopsis thaliana OX=3702 GN=CALS3 PE=3 SV=3[more]
Q9AUE05.7e-21565.75Callose synthase 1 OS=Arabidopsis thaliana OX=3702 GN=CALS1 PE=1 SV=2[more]
Q3B7242.4e-21367.09Callose synthase 5 OS=Arabidopsis thaliana OX=3702 GN=CALS5 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JJ301.2e-30094.431,3-beta-glucan synthase OS=Cucurbita maxima OX=3661 GN=LOC111486272 PE=3 SV=1[more]
A0A6J1EVT37.8e-30094.601,3-beta-glucan synthase OS=Cucurbita moschata OX=3662 GN=LOC111437035 PE=3 SV=1[more]
A0A6J1D4633.9e-29997.061,3-beta-glucan synthase OS=Momordica charantia OX=3673 GN=LOC111016827 PE=3 SV=... [more]
A0A1S3BG551.5e-29593.361,3-beta-glucan synthase OS=Cucumis melo OX=3656 GN=LOC103489654 PE=3 SV=1[more]
A0A0A0K4134.9e-29493.181,3-beta-glucan synthase OS=Cucumis sativus OX=3659 GN=Csa_7G236800 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G06490.12.6e-27182.59glucan synthase-like 7 [more]
AT3G59100.15.8e-25580.26glucan synthase-like 11 [more]
AT5G13000.11.0e-22266.42glucan synthase-like 12 [more]
AT1G05570.14.1e-21665.75callose synthase 1 [more]
AT1G05570.24.1e-21665.75callose synthase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003440Glycosyl transferase, family 48PFAMPF02364Glucan_synthasecoord: 12..365
e-value: 3.2E-120
score: 402.7
NoneNo IPR availablePANTHERPTHR12741LYST-INTERACTING PROTEIN LIP5 DOPAMINE RESPONSIVE PROTEIN DRG-1coord: 5..548
NoneNo IPR availablePANTHERPTHR12741:SF71GLYCOSYL TRANSFERASE, FAMILY 48-RELATEDcoord: 5..548

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg007811.1Spg007811.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006075 (1->3)-beta-D-glucan biosynthetic process
biological_process GO:0071555 cell wall organization
biological_process GO:0008360 regulation of cell shape
cellular_component GO:0000148 1,3-beta-D-glucan synthase complex
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0003843 1,3-beta-D-glucan synthase activity