Spg007196 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg007196
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Alpha-amylase
Location	scaffold9: 47376262 .. 47382238 (+)
RNA-Seq Expression	Spg007196
Synteny	Spg007196

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAAAATTAGCCAACACAAAACTAGCTCAATTGGTATAGAGTTTATACTAATGATCACAAAGTCCGTGATTCAAACCTCCCACCCTGTAAACGAAAGGAAAAACACAAACATAGCAATAGCATACGTTCAATAAAAAAAAAAAAAATGGGGTCTTCAACGGTTAGTATTCCATGAAACCCACAGTTGCTTAACGCTTAAGCCATTGCAATTACCCAAATAGCTGACCAATCATTTGATTGCAAATGGTGGACCGTATAACATCCGATTCCCTGAATCAGCAAAACCCAGAAAACGTCGAAGCGCGAAGTGTTCTTGAACACGAAACTCAACTGAGGTCATTGTTTAAAACTCCGATTCTTTCCTTGTTCATTCTGCGGGATTCTCAGCAGTCCGAATTTGTTACTTCTCGGTAGCGTTTCTCACTTGTTTACAGAGTAGTTTCTTTCACTGCCTCATATTATTTTCGGGTAGAAATGCGTGGTTTATAGCTATTTATCCATTGAACATGTATACGTGATGGATTACTTGAAGAATCCGTCGTTACATTTGCGTTTTTCTCCGTAGCTTTGTTTCTTCCATGCCATCTGAATTGAAGGTTTTTGCTGAAAGAAGCATTGTTTATTTAGTTCTATAACTTATATTATCTATTTCTTGATTTTATCGCTGGATCATAATTCCTCTTAAATATCTTCGCGCCAATCTGGAACTTGTGATTTTCAGTTGATTAATTCGTATTATCAAGGCTGATCGCATCTGCTGGTTGTTAGTTTAAACCTACGAAGGAGTTGATTTTGGACAGGTTTAAGGAGTGTCAATGTTTTATCCAATTTCAAATTGAGGAACAGATTCTCAAAATGAATTAGGTTGTTGATTGCCGCTTAGAGAATTAAGCTAGAGCCATGAATTGTCCGCACCACGTCACTAACTACTACCCTGTTGTAATTCTAGCTCGTTCCTTTAGTTTCCTTTTCCTTGTTCGAGTGGGCATTATGAAGTTATGACCAGACAATATGAATGAGGTACACTTTACAGAGTGTGATGATTTTTTCGTCTTTGATTGTGGTTGCAGGACATCCCTATTTTCTGCAACTTAGACGCCAAGGTTTCACAAGTTACTCCTGAGTATCATGGGTGACCATGATAATGTATGGTTCGTAATTAACGCATATGTCCATAGGGTTTTTGACGGATTATTTATGCACGTGGACAAACAAAAATTAGCTGAATTTGGAAATTTTTTCCTTGATGAATTTGTCACAAATTTGTTAGAACCTACTTTGTAATATACCAATTACTTATTTATGTTGGAATTCAGGGTTTTGACGACAGCTACGAGCAGACTGATACTGGTACGGCCTTTTGTTTGTTTTGACATTACATTTCACATCATGATAGCTCATGTTAATCTTTAAAGATCATACGTCCATCGGAAGTGACTTCAAATTCTAATACATGTACTCTGGCCTAGCTAACCAAGAGGAATGTTTGATGATGCTTAAAATTTAACTCAGGGATAAGAGATAAAAAGACATTATATACTTCTGAAGGATGGGTACGTTGAGCAATGACTTCAAAACAAAACTTATGGTTTATGGAAAGGATATGTACACATGGTTGAAATTAGAGTTGGTTTTCCTAATATGTAGATTTTGTTTTTTGTTTAAAAGTATCTTATCATTTAAAATTATTCCGGTCTTTTTGGTTACTTGTAGGGGCAGTGATACGCAATGGAAGAGAGATCCTTTTCCAGGTAGAGGTTACAATTTTAGCTTTCTGATTTTCCGTAGAACAGAGGTCAGCAGGAAGATATTTTGTTGCTCTTATTGGGGGCGAAGTCTCAAAAAAGAAAAGAAAGCAAATGATGCTTGGCTTTTGAAATTCGTCCTGGATGTTGTATCTTTTAATGTAAATCAGTTTGAAGTCGAGGTGGGAGGACTTCACTACTGTTAATGAGTCAATGTTTTTAGATCAAATCTCCAAGTCAGTTTAAACATATGTGATCAATTCAGCACTCATAGTTTTTACATTAATTACAGGCTTTCAACTGGGAATCTCATAAATGTGATTGGTGGAGGAATCTAGAGTGTAAAGTTCCCGATATTGCAAAATGTGGATTTACCTCTGTATGGTTGCCACCTCCAACTCATTCTATCGCTCCTGAAGGTCAAAGGAACATATTTACTTGAATTTTTGTTTTCCTTTTCCATGTATGTGTGCATTATAACTCTTGGTATTCTTTTAACAATTTCAGGTTACCTCCCTCAGAACCTGTACTCCCTAAACTCTTCATATGGTTCTGAGGACATGCTGAAAGCTTTACTTCAGAAAATGAAGCAGTGCAAGGTTAGAGCAATTGCTGACATAGTCATCAATCATCGAGTTGGGACTATTCAAGGACATGGAGGAATGCATAATCGTTTTGATGGAATACCATTACCATGGAATGAGAGTGCTGTTACCTCGTGCTCTGGTGGCCTGGTATTTATTTGTTACAAATTCCAAGTGAGCCCATAATTATCAGAAAACCTCTGATCTTTAGTCTCCGATATCTGTTATAGGGCAATCGTAGCACTGGGGACAACTTCCCTGGGGTTCCAAACATTGATCATAGCCAACCTTTTGTCCGTAGAGATATCATAGCATGGCTGAAGTGGCTGCGCAGTAGTGTTGGATTTCAGGATTTTCGTTTTGATTTTGCAAGGGGGTAATATTTTCCTATCTGTTTGTTTATATATAATATAATTTGGGGGTGGGGGGTTGCAAAGGTTCAAGAATAAATTATTTGGTATTACTCATTGCATAAAAATCTGTAAAATCTCCACTTTTGAACATGACAAGATCGCTCTTCTTTATTTCAAATCTTCACTCTCTATCATCTTCTCAACTTTTCATCTTGCCTGTTCTTGTAAGGAGTTCTTCAATTGGTTCTGATATACATTTGCCTTTTTCTTTTGTCTTTAACTCAGTTATTCTGCAAAATTCGTAAAAGAATATATTGAAGCAGCAAAGCCAATGTTTTCTATTGGAGAGTACTGGGATTCTTGTAATTATAATGGCCATGACTTGGATTATAATCAAGGTAAGATGGTGAATCTTTGTTCTTCGTATATAATATGGTGATAAATTGGAATCATGTTTTAAGCAATATCCCTAACCGTTTATTGGTTGCTTCAGGATATTTAGTTTATTTGAATGGTTGATGTTTTTGGATAATATCCTCTTTTTTCTTTTTTTTTTTTTTCCTCCCTCGATTCATGTATTCTATAGTGATGATCTCAACTCTAAATGCAGATAGACACAGACAGCGGATAATAAATTGGATTGATAGCACGGGACAACTTTCATCTGCTTTTGATTTTACAACCAAGGGAATTCTTCAGGTATCAGTGTTGTATGTGGGCCATTGCTAACCCCCCTCCCCCTCCAAATTGTAATTACTAAAGATATCAAATCTTAATTTTTTAAAATAGAAAGGGTCAGGCGACAATAATGTCATGGATTTTCATTTCCCTATGGATCTAGATCTGCAACGTTTGGCTTTTCATGAACTTTTCCATTTGCTTCTTTGTGCCTTTCATATTAAGTGAAGTTTGGATTGCGCTAATATTTTCTCTTGCCAGTACAATAATTAACTAACATGCATTTGTCTCCTTGTTTACTGCTTAAAAAACATAATTTAATTAATTTGGCTTGTAGGAAGCTGTTAAAGGACAATTATGGCGTCTGCGTGACTGTCAAGGAAAGCCACCAGGTGTGATGGGATGGTGGCCTTCTAGGGCCGTAACATTCTTAGATAACCATGATACTGGTTCAACACAGGTGCCACATCTTCATAACTATCTGTGAAATTATTGATCGACTGTTTGGGAATGGATAAAAATTTCAACATTTGGGTTTGCGAGATAAAATGGTCACTATTTGATCATATTTCTTGCTAGCCATGATCCTTTTATTTGGATTAATATTTGATGGCACGTGTTTGGAAGTCATGCCTTGTAGTGCGGATTGAGTGTGACCAGACCGTACATGAAAATATCTTAGAAAGCAATGGAGAAGAGCATTATTGATGCATACCTGAATGAACTATGAAGTTATAATGTCTGGTTGTCCTGCGGCTTTTATATGGCCAAATATGCTGCCAACAGAACAGTTACTGGATTCTGTGGCATTTGATAGTGATGCTTTCATATTATTGGAGGGTATTATCTTTCGAAGATGTGTATGCATTTTTGCAACAACATATAACTGAGATGTATGAGGCCAAGCTCAAAGTATATTTGAAATTTTTAGTTTTCTTTTTCTTCCATAGGTGTGTTTGAATTTGAACTAATTAAAATTCAAGTGTGCTAGTAAACTCAGCATCTTCAAGTGTAGCTTTGGATTATTATTTGGAAACTCTTAATTAACTTCTTCTTCTTCTTCTTCTTTTTTTTTTCCCCTAGTTTCTAAATTGGTGGTTGTTTGATGCATATTATATTTGGTTACATTTTTTAGTGATGTTTTGAAGCTTACCTATGAAAGATCAACTATCTTCTCATCCCCTTACTATGAGAAGGGTTAAGGTTGAAAGCAAGCATCAGGCGTGCTTTCAATAATAGCAATGGGCTAAGCAGATCATGACTATTAATGCAGCTCTACTTGCTTGGCAACCGAACCTATGTAATTCTTCATATATATTTTCTGGAAATATATTTTGAAGTTGAATTTGTACCACTAGAATGTTAGTGACTCCACTAACCTCAGCATGCAATGTTGTTCTTGTAGGCCCATTGGCCCTTTCCTGCCAACCATATTATGGAGGTATGCAAACTCTGTCATCACTGAATTTGTAATCTCTCTTTCTTGCTTGCAGTCTAATTTTAGCTGCGCTGTGGCTTTCTTTCATGACATGCCTGTCCTGGTTTAATAGTAAACTCTACATACCTAATCACGTGTTCATCATCATTCGTTGTGGTTTTTGGAAACTCTCAATTGTTTGGTCCAACTAATCCTATGTATGACTCCAGCCCCATCTTCTTCCAACATTGGATGTTTTTAAGCAAGTTATTCCCAAAGATTTGACTACATTTTAAGTTCATTTTCAACTTTTAACAAAATGTTTTACATGCTGAGTGATCAATCCTTGATGTTTCTGGGTTAACTGCGTTAGAAGAGCATTGTATTTATTTTCTCGTCTTACAGTTGCTATCGCAACTACTTTTATGCTCGACCTAAAGTCATGTCTTGTCTTCCAATAATAAAATATCATCATGCATCTAATTTTGCTTTCTCGAGCTCTGGTTTATTAGCCCATAATTCCCTGCAGGGCTATGCATATATTCTCACGCACCCAGGGATACCAACAGTATTTTATGACCACTTCTACGATTGGGGCGACTCGGTGCACAGAGAAATAGTAAAATTAGTATGTTGCATGATTAATGCTTATTGAGTTTGCTATCCGCTCTTCAAGAAACTCTTCACGGTACATAATTCTATTTTTCTTGCAGATAGACATTCGTAAACGTCAAGACATCTACAGTCGGTCATCTGTGAAGATCCTTGAGGCACGATCAGATCTTTATTCTGCAATAATTGGGGAGAAATTGTGCATGAAAATCGGAGATGGCTCGTGGTGCCCTTCTGGTACGGAATGGACTCTTGCAACCTCTGGCCGCAGATATGCGGTATGGCAGAAATAAGGTTTGAAAGTTTTACCTTCTTTCCCCAACCTTCTTTGTTTCTTTTCTGCCATCGGGGATTACATGTAAACTGTAAAGTTATATAATCAGTTGGCTACTCATAGTATACAATCATAAATAGTCATTGTGGATGAAATTAATGCATTTTCACATAATCGATTATATCATATCGCTTATTTTACTGACTCTCGAGGAACTTTACATACAGCACGTTGTCAAGTCTGATAATAGGAAAGATTATTATCTTCAAGCAAAATAATCTTCCCTGTGTGGAGCTTTGAGTGCTATATATGGT

mRNA sequence

ATGGTGGACCGTATAACATCCGATTCCCTGAATCAGCAAAACCCAGAAAACGTCGAAGCGCGAAGTGTTCTTGAACACGAAACTCAACTGAGGTCATTGTTTAAAACTCCGATTCTTTCCTTGTTCATTCTGCGGGATTCTCAGCAGTCCGAATTTGTTACTTCTCGCTTTGTTTCTTCCATGCCATCTGAATTGAAGGACATCCCTATTTTCTGCAACTTAGACGCCAAGGGTTTTGACGACAGCTACGAGCAGACTGATACTGGTACGGCCTTTTGTTTGGGCAGTGATACGCAATGGAAGAGAGATCCTTTTCCAGGTAGAGGTTACAATTTTAGCTTTCTGATTTTCCGTAGAACAGAGGTCAGCAGGAAGATATTTTGTTGCTCTTATTGGGGGCGAAGTCTCAAAAAAGAAAAGAAAGCAAATGATGCTTGGCTTTTGAAATTCGTCCTGGATGTTGTATCTTTTAATGTAAATCAGTTTGAAGTCGAGGCTTTCAACTGGGAATCTCATAAATGTGATTGGTGGAGGAATCTAGAGTGTAAAGTTCCCGATATTGCAAAATGTGGATTTACCTCTGTATGGTTGCCACCTCCAACTCATTCTATCGCTCCTGAAGGTTACCTCCCTCAGAACCTGTACTCCCTAAACTCTTCATATGGTTCTGAGGACATGCTGAAAGCTTTACTTCAGAAAATGAAGCAGTGCAAGGTTAGAGCAATTGCTGACATAGTCATCAATCATCGAGTTGGGACTATTCAAGGACATGGAGGAATGCATAATCGTTTTGATGGAATACCATTACCATGGAATGAGAGTGCTGTTACCTCGTGCTCTGGTGGCCTGGGCAATCGTAGCACTGGGGACAACTTCCCTGGGGTTCCAAACATTGATCATAGCCAACCTTTTGTCCGTAGAGATATCATAGCATGGCTGAAGTGGCTGCGCAGTAGTGTTGGATTTCAGGATTTTCGTTTTGATTTTGCAAGGGGTTATTCTGCAAAATTCGTAAAAGAATATATTGAAGCAGCAAAGCCAATGTTTTCTATTGGAGAGTACTGGGATTCTTGTAATTATAATGGCCATGACTTGGATTATAATCAAGATAGACACAGACAGCGGATAATAAATTGGATTGATAGCACGGGACAACTTTCATCTGCTTTTGATTTTACAACCAAGGGAATTCTTCAGGAAGCTGTTAAAGGACAATTATGGCGTCTGCGTGACTGTCAAGGAAAGCCACCAGGTGTGATGGGATGGTGGCCTTCTAGGGCCGTAACATTCTTAGATAACCATGATACTGGTTCAACACAGGCCCATTGGCCCTTTCCTGCCAACCATATTATGGAGGGCTATGCATATATTCTCACGCACCCAGGGATACCAACAGTATTTTATGACCACTTCTACGATTGGGGCGACTCGGTGCACAGAGAAATAGTAAAATTAATAGACATTCGTAAACGTCAAGACATCTACAGTCGGTCATCTGTGAAGATCCTTGAGGCACGATCAGATCTTTATTCTGCAATAATTGGGGAGAAATTGTGCATGAAAATCGGAGATGGCTCGTGGTGCCCTTCTGGTACGGAATGGACTCTTGCAACCTCTGGCCGCAGATATGCGGTATGGCAGAAATAA

Coding sequence (CDS)

ATGGTGGACCGTATAACATCCGATTCCCTGAATCAGCAAAACCCAGAAAACGTCGAAGCGCGAAGTGTTCTTGAACACGAAACTCAACTGAGGTCATTGTTTAAAACTCCGATTCTTTCCTTGTTCATTCTGCGGGATTCTCAGCAGTCCGAATTTGTTACTTCTCGCTTTGTTTCTTCCATGCCATCTGAATTGAAGGACATCCCTATTTTCTGCAACTTAGACGCCAAGGGTTTTGACGACAGCTACGAGCAGACTGATACTGGTACGGCCTTTTGTTTGGGCAGTGATACGCAATGGAAGAGAGATCCTTTTCCAGGTAGAGGTTACAATTTTAGCTTTCTGATTTTCCGTAGAACAGAGGTCAGCAGGAAGATATTTTGTTGCTCTTATTGGGGGCGAAGTCTCAAAAAAGAAAAGAAAGCAAATGATGCTTGGCTTTTGAAATTCGTCCTGGATGTTGTATCTTTTAATGTAAATCAGTTTGAAGTCGAGGCTTTCAACTGGGAATCTCATAAATGTGATTGGTGGAGGAATCTAGAGTGTAAAGTTCCCGATATTGCAAAATGTGGATTTACCTCTGTATGGTTGCCACCTCCAACTCATTCTATCGCTCCTGAAGGTTACCTCCCTCAGAACCTGTACTCCCTAAACTCTTCATATGGTTCTGAGGACATGCTGAAAGCTTTACTTCAGAAAATGAAGCAGTGCAAGGTTAGAGCAATTGCTGACATAGTCATCAATCATCGAGTTGGGACTATTCAAGGACATGGAGGAATGCATAATCGTTTTGATGGAATACCATTACCATGGAATGAGAGTGCTGTTACCTCGTGCTCTGGTGGCCTGGGCAATCGTAGCACTGGGGACAACTTCCCTGGGGTTCCAAACATTGATCATAGCCAACCTTTTGTCCGTAGAGATATCATAGCATGGCTGAAGTGGCTGCGCAGTAGTGTTGGATTTCAGGATTTTCGTTTTGATTTTGCAAGGGGTTATTCTGCAAAATTCGTAAAAGAATATATTGAAGCAGCAAAGCCAATGTTTTCTATTGGAGAGTACTGGGATTCTTGTAATTATAATGGCCATGACTTGGATTATAATCAAGATAGACACAGACAGCGGATAATAAATTGGATTGATAGCACGGGACAACTTTCATCTGCTTTTGATTTTACAACCAAGGGAATTCTTCAGGAAGCTGTTAAAGGACAATTATGGCGTCTGCGTGACTGTCAAGGAAAGCCACCAGGTGTGATGGGATGGTGGCCTTCTAGGGCCGTAACATTCTTAGATAACCATGATACTGGTTCAACACAGGCCCATTGGCCCTTTCCTGCCAACCATATTATGGAGGGCTATGCATATATTCTCACGCACCCAGGGATACCAACAGTATTTTATGACCACTTCTACGATTGGGGCGACTCGGTGCACAGAGAAATAGTAAAATTAATAGACATTCGTAAACGTCAAGACATCTACAGTCGGTCATCTGTGAAGATCCTTGAGGCACGATCAGATCTTTATTCTGCAATAATTGGGGAGAAATTGTGCATGAAAATCGGAGATGGCTCGTGGTGCCCTTCTGGTACGGAATGGACTCTTGCAACCTCTGGCCGCAGATATGCGGTATGGCAGAAATAA

Protein sequence

MVDRITSDSLNQQNPENVEARSVLEHETQLRSLFKTPILSLFILRDSQQSEFVTSRFVSSMPSELKDIPIFCNLDAKGFDDSYEQTDTGTAFCLGSDTQWKRDPFPGRGYNFSFLIFRRTEVSRKIFCCSYWGRSLKKEKKANDAWLLKFVLDVVSFNVNQFEVEAFNWESHKCDWWRNLECKVPDIAKCGFTSVWLPPPTHSIAPEGYLPQNLYSLNSSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTIQGHGGMHNRFDGIPLPWNESAVTSCSGGLGNRSTGDNFPGVPNIDHSQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGYSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDRHRQRIINWIDSTGQLSSAFDFTTKGILQEAVKGQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHREIVKLIDIRKRQDIYSRSSVKILEARSDLYSAIIGEKLCMKIGDGSWCPSGTEWTLATSGRRYAVWQK

Homology

[Close]

BLAST of Spg007196 vs. NCBI nr
Match: KAA0064621.1 (putative alpha-amylase 2 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 765.4 bits (1975), Expect = 3.4e-217
Identity = 355/391 (90.79%), Postives = 366/391 (93.61%), Query Frame = 0


Query: 158 NVNQFEVEAFNWESHKCDWWRNLECKVPDIAKCGFTSVWLPPPTHSIAPEGYLPQNLYSL 217
           N  +   +AFNWESHKCDW  NLECKV DIAK GFTS WLPPPTHSIAP+GYLPQNLYSL
Sbjct: 78  NGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSL 137

Query: 218 NSSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTIQGHGGMHNRFDGIPLPWNESAVT 277
           NSSYGSEDMLKALL KMKQCKVRA+ADIVINHRVGT+ GHGGMHNRFDGI LPW+E AVT
Sbjct: 138 NSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAVT 197

Query: 278 SCSGGLGNRSTGDNFPGVPNIDHSQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGYSAKF 337
           SCSGGLGNRSTGDNF GVPNIDH+QPFVRRDIIAWLKWLRSSVGFQDFRFDFARG+SAKF
Sbjct: 198 SCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGFSAKF 257

Query: 338 VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDRHRQRIINWIDSTGQLSSAFDFTTKGI 397
           VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQD HRQRIINWID TGQLS+AFDFTTKG+
Sbjct: 258 VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFTTKGV 317

Query: 398 LQEAVKGQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYI 457
           LQEAVKGQLWRL DC+GKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYI
Sbjct: 318 LQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYI 377

Query: 458 LTHPGIPTVFYDHFYDWGDSVHREIVKLIDIRKRQDIYSRSSVKILEARSDLYSAIIGEK 517
           LTHPGIPTVFYDHFYDWGDSVH EIVKLIDIRK QDI SRSSVKILEARSDLYSAIIGE 
Sbjct: 378 LTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDLYSAIIGEN 437

Query: 518 LCMKIGDGSWCPSGTEWTLATSGRRYAVWQK 549
           LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 438 LCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 467

[Close]

BLAST of Spg007196 vs. NCBI nr
Match: XP_008452915.1 (PREDICTED: probable alpha-amylase 2 isoform X2 [Cucumis melo])

HSP 1 Score: 765.4 bits (1975), Expect = 3.4e-217
Identity = 355/391 (90.79%), Postives = 366/391 (93.61%), Query Frame = 0


Query: 158 NVNQFEVEAFNWESHKCDWWRNLECKVPDIAKCGFTSVWLPPPTHSIAPEGYLPQNLYSL 217
           N  +   +AFNWESHKCDW  NLECKV DIAK GFTS WLPPPTHSIAP+GYLPQNLYSL
Sbjct: 48  NGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSL 107

Query: 218 NSSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTIQGHGGMHNRFDGIPLPWNESAVT 277
           NSSYGSEDMLKALL KMKQCKVRA+ADIVINHRVGT+ GHGGMHNRFDGI LPW+E AVT
Sbjct: 108 NSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAVT 167

Query: 278 SCSGGLGNRSTGDNFPGVPNIDHSQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGYSAKF 337
           SCSGGLGNRSTGDNF GVPNIDH+QPFVRRDIIAWLKWLRSSVGFQDFRFDFARG+SAKF
Sbjct: 168 SCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGFSAKF 227

Query: 338 VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDRHRQRIINWIDSTGQLSSAFDFTTKGI 397
           VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQD HRQRIINWID TGQLS+AFDFTTKG+
Sbjct: 228 VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFTTKGV 287

Query: 398 LQEAVKGQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYI 457
           LQEAVKGQLWRL DC+GKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYI
Sbjct: 288 LQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYI 347

Query: 458 LTHPGIPTVFYDHFYDWGDSVHREIVKLIDIRKRQDIYSRSSVKILEARSDLYSAIIGEK 517
           LTHPGIPTVFYDHFYDWGDSVH EIVKLIDIRK QDI SRSSVKILEARSDLYSAIIGE 
Sbjct: 348 LTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDLYSAIIGEN 407

Query: 518 LCMKIGDGSWCPSGTEWTLATSGRRYAVWQK 549
           LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 408 LCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 437

[Close]

BLAST of Spg007196 vs. NCBI nr
Match: XP_011654252.1 (probable alpha-amylase 2 [Cucumis sativus] >XP_031739646.1 probable alpha-amylase 2 [Cucumis sativus])

HSP 1 Score: 764.6 bits (1973), Expect = 5.8e-217
Identity = 352/391 (90.03%), Postives = 364/391 (93.09%), Query Frame = 0


Query: 158 NVNQFEVEAFNWESHKCDWWRNLECKVPDIAKCGFTSVWLPPPTHSIAPEGYLPQNLYSL 217
           N  +   +AF+WESHK DWW NLE KV DIAK GFTS WLPPPTHSIAP+GYLPQNLYSL
Sbjct: 23  NGREILFQAFSWESHKYDWWENLESKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSL 82

Query: 218 NSSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTIQGHGGMHNRFDGIPLPWNESAVT 277
           NSSYGSEDMLK LL KMKQCKVRAIADIVINHRVGT+ GHGGMHNRFDGI LPWNE AVT
Sbjct: 83  NSSYGSEDMLKTLLLKMKQCKVRAIADIVINHRVGTVVGHGGMHNRFDGILLPWNEYAVT 142

Query: 278 SCSGGLGNRSTGDNFPGVPNIDHSQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGYSAKF 337
           SCSGGLGNRSTGDNF GVPNIDH+QPFVRRDIIAWLKWLRSSVGFQDFRFDFARG+SAKF
Sbjct: 143 SCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGFSAKF 202

Query: 338 VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDRHRQRIINWIDSTGQLSSAFDFTTKGI 397
           VKEYIEA KPM SIGEYWDSCNYNGHDLDYNQD HRQRIINWID TGQLS+AFDFTTKG+
Sbjct: 203 VKEYIEAGKPMLSIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFTTKGV 262

Query: 398 LQEAVKGQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYI 457
           LQEAV+ QLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYI
Sbjct: 263 LQEAVRRQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYI 322

Query: 458 LTHPGIPTVFYDHFYDWGDSVHREIVKLIDIRKRQDIYSRSSVKILEARSDLYSAIIGEK 517
           LTHPGIPTVFYDH Y+WGDSVH EIV+LIDIRK QDI SRSSVKILEARSDLYSAIIGEK
Sbjct: 323 LTHPGIPTVFYDHLYEWGDSVHDEIVRLIDIRKHQDINSRSSVKILEARSDLYSAIIGEK 382

Query: 518 LCMKIGDGSWCPSGTEWTLATSGRRYAVWQK 549
           LCMKIGDGSWCPSGTEWTLATSG RYAVWQK
Sbjct: 383 LCMKIGDGSWCPSGTEWTLATSGWRYAVWQK 413

[Close]

BLAST of Spg007196 vs. NCBI nr
Match: ADN34132.1 (cytosolic alpha-amylase [Cucumis melo subsp. melo])

HSP 1 Score: 759.6 bits (1960), Expect = 1.9e-215
Identity = 355/395 (89.87%), Postives = 366/395 (92.66%), Query Frame = 0


Query: 158 NVNQFEVEAFNWESHKCDWWRNLECKVPDIAKCGFTSVWLPPPTHSIAPEGYLPQNLYSL 217
           N  +   +AFNWESHKCDW  NLECKV DIAK GFTS WLPPPTHSIAP+GYLPQNLYSL
Sbjct: 23  NGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSL 82

Query: 218 NSSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTIQGHGGMHNRFDGIPLPWNESAVT 277
           NSSYGSEDMLKALL KMKQCKVRA+ADIVINHRVGT+ GHGGMHNRFDGI LPW+E AVT
Sbjct: 83  NSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAVT 142

Query: 278 SCSGGLGNRSTGDNFPGVPNIDHSQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGYSAKF 337
           SCSGGLGNRSTGDNF GVPNIDH+QPFVRRDIIAWLKWLRSSVGFQDFRFDFARG+SAKF
Sbjct: 143 SCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGFSAKF 202

Query: 338 VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ----DRHRQRIINWIDSTGQLSSAFDFT 397
           VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ    D HRQRIINWID TGQLS+AFDFT
Sbjct: 203 VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQALNADGHRQRIINWIDGTGQLSAAFDFT 262

Query: 398 TKGILQEAVKGQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEG 457
           TKG+LQEAVKGQLWRL DC+GKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEG
Sbjct: 263 TKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEG 322

Query: 458 YAYILTHPGIPTVFYDHFYDWGDSVHREIVKLIDIRKRQDIYSRSSVKILEARSDLYSAI 517
           YAYILTHPGIPTVFYDHFYDWGDSVH EIVKLIDIRK QDI SRSSVKILEARSDLYSAI
Sbjct: 323 YAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDLYSAI 382

Query: 518 IGEKLCMKIGDGSWCPSGTEWTLATSGRRYAVWQK 549
           IGE LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 383 IGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 416

[Close]

BLAST of Spg007196 vs. NCBI nr
Match: TYK19971.1 (putative alpha-amylase 2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 759.6 bits (1960), Expect = 1.9e-215
Identity = 355/395 (89.87%), Postives = 366/395 (92.66%), Query Frame = 0


Query: 158 NVNQFEVEAFNWESHKCDWWRNLECKVPDIAKCGFTSVWLPPPTHSIAPEGYLPQNLYSL 217
           N  +   +AFNWESHKCDW  NLECKV DIAK GFTS WLPPPTHSIAP+GYLPQNLYSL
Sbjct: 78  NGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSL 137

Query: 218 NSSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTIQGHGGMHNRFDGIPLPWNESAVT 277
           NSSYGSEDMLKALL KMKQCKVRA+ADIVINHRVGT+ GHGGMHNRFDGI LPW+E AVT
Sbjct: 138 NSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAVT 197

Query: 278 SCSGGLGNRSTGDNFPGVPNIDHSQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGYSAKF 337
           SCSGGLGNRSTGDNF GVPNIDH+QPFVRRDIIAWLKWLRSSVGFQDFRFDFARG+SAKF
Sbjct: 198 SCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGFSAKF 257

Query: 338 VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ----DRHRQRIINWIDSTGQLSSAFDFT 397
           VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ    D HRQRIINWID TGQLS+AFDFT
Sbjct: 258 VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQALNADGHRQRIINWIDGTGQLSAAFDFT 317

Query: 398 TKGILQEAVKGQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEG 457
           TKG+LQEAVKGQLWRL DC+GKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEG
Sbjct: 318 TKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEG 377

Query: 458 YAYILTHPGIPTVFYDHFYDWGDSVHREIVKLIDIRKRQDIYSRSSVKILEARSDLYSAI 517
           YAYILTHPGIPTVFYDHFYDWGDSVH EIVKLIDIRK QDI SRSSVKILEARSDLYSAI
Sbjct: 378 YAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDLYSAI 437

Query: 518 IGEKLCMKIGDGSWCPSGTEWTLATSGRRYAVWQK 549
           IGE LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 438 IGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 471

[Close]

BLAST of Spg007196 vs. ExPASy Swiss-Prot
Match: Q8LFG1 (Probable alpha-amylase 2 OS=Arabidopsis thaliana OX=3702 GN=AMY2 PE=2 SV=1)

HSP 1 Score: 706.4 bits (1822), Expect = 2.4e-202
Identity = 310/385 (80.52%), Postives = 357/385 (92.73%), Query Frame = 0


Query: 164 VEAFNWESHKCDWWRNLECKVPDIAKCGFTSVWLPPPTHSIAPEGYLPQNLYSLNSSYGS 223
           ++A+NWESHK DWWRNL+ KVPDIAK GFTS WLPPP+ S+APEGYLPQ+LYSLNS+YGS
Sbjct: 29  LQAYNWESHKYDWWRNLDGKVPDIAKSGFTSAWLPPPSQSLAPEGYLPQDLYSLNSAYGS 88

Query: 224 EDMLKALLQKMKQCKVRAIADIVINHRVGTIQGHGGMHNRFDGIPLPWNESAVTSCSGGL 283
           E +LK+LL+KMKQ KVRA+ADIVINHRVGT +GHGGM+NR+DGI LPW+E AVTSC+GGL
Sbjct: 89  EHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDGISLPWDEHAVTSCTGGL 148

Query: 284 GNRSTGDNFPGVPNIDHSQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGYSAKFVKEYIE 343
           GNRSTGDNF GVPN+DH+Q FVR+DII WL+WLR++VGFQDFRFDFARGYSA +VKEYI 
Sbjct: 149 GNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQDFRFDFARGYSANYVKEYIG 208

Query: 344 AAKPMFSIGEYWDSCNYNGHDLDYNQDRHRQRIINWIDSTGQLSSAFDFTTKGILQEAVK 403
           AAKP+FS+GE WDSCNYNGH LDYNQD HRQRII+WID+TGQ+S+AFDFTTKGILQEAVK
Sbjct: 209 AAKPLFSVGECWDSCNYNGHGLDYNQDSHRQRIISWIDATGQISAAFDFTTKGILQEAVK 268

Query: 404 GQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYILTHPGI 463
           GQ WRL D QGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFP++H+MEGYAYILTHPGI
Sbjct: 269 GQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPSHHVMEGYAYILTHPGI 328

Query: 464 PTVFYDHFYDWGDSVHREIVKLIDIRKRQDIYSRSSVKILEARSDLYSAIIGEKLCMKIG 523
           P VFYDHFYDWG S+H +IVKLIDIR+RQDI+SRS+V++L+A S+LY+AI+GEK+CMK+G
Sbjct: 329 PCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAIVGEKICMKLG 388

Query: 524 DGSWCPSGTEWTLATSGRRYAVWQK 549
           DGSWCPSG +WTLATSG RYAVW K
Sbjct: 389 DGSWCPSGRDWTLATSGHRYAVWHK 413

[Close]

BLAST of Spg007196 vs. ExPASy Swiss-Prot
Match: Q94A41 (Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 1.9e-117
Identity = 199/395 (50.38%), Postives = 273/395 (69.11%), Query Frame = 0


Query: 162 FEV--EAFNWESHKCD-WWRNLECKVPDIAKCGFTSVWLPPPTHSIAPEGYLPQNLYSLN 221
           FE+  + FNWES+K   W+  L+ K  ++A  GFT +WLPPPT S++PEGY+P++LY+LN
Sbjct: 495 FEILCQGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYNLN 554

Query: 222 SSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTIQGHGGMHNRFDGIPLPWNESAVTS 281
           S YG+ D LK  ++K  +  ++ + D V+NHR    +   G+ N F G  L W++ AV +
Sbjct: 555 SRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGG-RLNWDDRAVVA 614

Query: 282 CS---GGLGNRSTGDNFPGVPNIDHSQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGYSA 341
                 G GN+S+GDNF   PNIDHSQ FVR+DI  WL W+   VG+  +R DF RG+  
Sbjct: 615 DDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRLDFVRGFWG 674

Query: 342 KFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDRHRQRIINWIDSTGQLSSAFDFTTK 401
            +VK+Y++A+KP F++GEYWDS +Y   ++DYNQD HRQRI++WI++T   + AFD TTK
Sbjct: 675 GYVKDYMDASKPYFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAAGAFDVTTK 734

Query: 402 GILQEAV-KGQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGY 461
           GIL  A+ K + WRL D +GKPPGV+GWWPSRAVTF++NHDTGSTQ HW FP    M+GY
Sbjct: 735 GILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPEGKEMQGY 794

Query: 462 AYILTHPGIPTVFYDHFYDWGDSVHREIVKLIDIRKRQDIYSRSSVKILEARSDLYSAII 521
           AYILTHPG P VF+DH +      H EI  L+ +R RQ ++ RS V I ++  D+Y+AII
Sbjct: 795 AYILTHPGTPAVFFDHIF---SDYHSEIAALLSLRNRQKLHCRSEVNIDKSERDVYAAII 854

Query: 522 GEKLCMKIGDGSW-CPSGTE-WTLATSGRRYAVWQ 548
            EK+ MKIG G +  P+G++ W++A  GR Y VW+
Sbjct: 855 DEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWE 885

[Close]

BLAST of Spg007196 vs. ExPASy Swiss-Prot
Match: P17859 (Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 2.3e-107
Identity = 191/399 (47.87%), Postives = 264/399 (66.17%), Query Frame = 0


Query: 165 EAFNWE-SHKCDWWRNLECKVPDIAKCGFTSVWLPPPTHSIAPEGYLPQNLYSLNSS-YG 224
           + FNWE S K  W+ +L+  +PD+A  G T VWLPPP+ S++PEGYLP  LY L++S YG
Sbjct: 28  QGFNWESSKKGGWYNSLKNSIPDLANAGITHVWLPPPSQSVSPEGYLPGRLYDLDASKYG 87

Query: 225 SEDMLKALLQKMKQCKVRAIADIVINHRVGTIQGHGGMHNRFDG----IPLPWNESAV-- 284
           S++ LK+L+    +  ++ +ADIVINHR    +   G++  F+G        W  S +  
Sbjct: 88  SKNELKSLIAAFHEKGIKCLADIVINHRTAERKDGRGIYCIFEGGTPDSRQDWGPSFICR 147

Query: 285 --TSCSGGLGNRSTGDNFPGVPNIDHSQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGYS 344
             T+ S G GN  +G+ +   P+IDH  P V+R++  W+ WL++ +GF  +RFDF +GY+
Sbjct: 148 DDTAYSDGTGNNDSGEGYDAAPDIDHLNPQVQRELSEWMNWLKTEIGFDGWRFDFVKGYA 207

Query: 345 AKFVKEYIEAAKPMFSIGEYWDSCNYNGHD--LDYNQDRHRQRIINWIDSTGQLSSAFDF 404
               K Y+E  KP F++GE WDS +Y G D   +YNQD HR  ++NW++S G   +AFDF
Sbjct: 208 PSISKIYMEQTKPDFAVGEKWDSISY-GQDGKPNYNQDSHRGALVNWVESAGGAITAFDF 267

Query: 405 TTKGILQEAVKGQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIME 464
           TTKGILQ AV+G+LWRL D  GKPPG++G  P  AVTF+DNHDTGSTQ  WPFP++ +M+
Sbjct: 268 TTKGILQAAVQGELWRLIDPNGKPPGMIGVKPENAVTFIDNHDTGSTQRLWPFPSDKVMQ 327

Query: 465 GYAYILTHPGIPTVFYDHFYDWGDSVHREIVKLIDIRKRQDIYSRSSVKILEARSDLYSA 524
           GYAYILTHPG P++FYDHF+DWG  +  +I KL  IR R  I  +S+VKI+ +  DLY A
Sbjct: 328 GYAYILTHPGTPSIFYDHFFDWG--LKEQIAKLSSIRLRNGINEKSTVKIMASEGDLYVA 387

Query: 525 IIGEKLCMKIGD----GSWCPSGTEWTLATSGRRYAVWQ 548
            I  K+ +KIG     G+  PS     +ATSG+ YAVW+
Sbjct: 388 KIDNKIMVKIGPKMDLGNLIPSNLH--VATSGQDYAVWE 421

[Close]

BLAST of Spg007196 vs. ExPASy Swiss-Prot
Match: P27934 (Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 1.8e-104
Identity = 187/400 (46.75%), Postives = 256/400 (64.00%), Query Frame = 0


Query: 165 EAFNWESHKCD--WWRNLECKVPDIAKCGFTSVWLPPPTHSIAPEGYLPQNLYSLNSS-Y 224
           + FNWES +    W+  L  KV +IA  G T VWLPPP+HS++P+GY+P  LY L++S Y
Sbjct: 30  QGFNWESWRKQGGWYNFLHEKVEEIASTGATHVWLPPPSHSVSPQGYMPGRLYDLDASKY 89

Query: 225 GSEDMLKALLQKMKQCKVRAIADIVINHRVGTIQGHGGMHNRFD-GIP---LPWNESAV- 284
           G+E  LK+L++      V  +ADIVINHR    +   G++  F+ G P   L W    + 
Sbjct: 90  GTEAELKSLIEAFHDKNVECLADIVINHRCADYKDSRGVYCVFEGGTPDGRLDWGPDMIC 149

Query: 285 ---TSCSGGLGNRSTGDNFPGVPNIDHSQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGY 344
              T  S G G+R TG  F   P+IDH  P V+R++  WL WLR+ +GF  +R DFA+GY
Sbjct: 150 SDDTQYSNGRGHRDTGAGFGAAPDIDHLNPRVQRELTDWLNWLRTDLGFDGWRLDFAKGY 209

Query: 345 SAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDL-DYNQDRHRQRIINWIDSTGQLSSAFDF 404
           SA   + Y++   P F +GE W S  YNG      NQD  RQ ++NW++  G+ ++AFDF
Sbjct: 210 SAPLARIYVDNTNPTFVVGEIWSSLIYNGDGKPSTNQDADRQELVNWVEGVGKPATAFDF 269

Query: 405 TTKGILQEAVKGQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIME 464
           TTKGILQ AV+G+LWRL D  GK PG+MGW P +AVTF+DNHDTGSTQ+ WPFP++ +M+
Sbjct: 270 TTKGILQAAVQGELWRLHDGNGKAPGLMGWMPDQAVTFVDNHDTGSTQSLWPFPSDKVMQ 329

Query: 465 GYAYILTHPGIPTVFYDHFYDWGDSVHREIVKLIDIRKRQDIYSRSSVKILEARSDLYSA 524
           GYAYILTHPGIP +FYDH +DW  ++  EI  L +IR R  I++ S++ IL+A  D+Y A
Sbjct: 330 GYAYILTHPGIPCIFYDHVFDW--NLQHEIATLAEIRSRNGIHAESTLDILKAEGDIYVA 389

Query: 525 IIGEKLCMKIGD----GSWCPSGTEWTLATSGRRYAVWQK 549
           +I  K+  K+G     G   PS  ++ +   G  Y VW+K
Sbjct: 390 MIDGKVITKLGPRYDAGGIIPS--DFHVVAHGNDYCVWEK 425

[Close]

BLAST of Spg007196 vs. ExPASy Swiss-Prot
Match: P27933 (Alpha-amylase isozyme 3D OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.3 PE=2 SV=2)

HSP 1 Score: 380.9 bits (977), Expect = 2.4e-104
Identity = 192/420 (45.71%), Postives = 269/420 (64.05%), Query Frame = 0


Query: 147 LLKFVLDVVSFNVNQFEV--EAFNWESHKCD--WWRNLECKVPDIAKCGFTSVWLPPPTH 206
           LL  VL  ++ N  Q +V  + FNWES K    W+  L+ +V DIAK G T VWLPPP+H
Sbjct: 10  LLLVVLCSLTCNSGQAQVLFQGFNWESWKQQGGWYNMLKGQVDDIAKAGVTHVWLPPPSH 69

Query: 207 SIAPEGYLPQNLYSLNSS-YGSEDMLKALLQKMKQCKVRAIADIVINHRVGTIQGHGGMH 266
           S+AP+GY+P  LY L++S YG+   LK+L+       V+ +AD+VINHR    +   G++
Sbjct: 70  SVAPQGYMPGRLYDLDASKYGTAAELKSLIAAFHGKGVQCVADVVINHRCAEKKDARGVY 129

Query: 267 NRFDG----IPLPWNESAV----TSCSGGLGNRSTGDNFPGVPNIDHSQPFVRRDIIAWL 326
             F+G      L W    +    T  S G G+R TG+ F   P+IDH  P V+R++  WL
Sbjct: 130 CVFEGGTPDDRLDWGPGMICSDDTQYSDGTGHRDTGEGFGAAPDIDHLNPRVQRELTDWL 189

Query: 327 KWLRSSVGFQDFRFDFARGYSAKFVKEYIEAAKPMFSIGEYWDSCNYNGHDLD-YNQDRH 386
            WL+S VGF  +R DFA+GYS    K Y+E+ KP F + E W+S +YNG      NQD+ 
Sbjct: 190 NWLKSDVGFDGWRLDFAKGYSTDIAKMYVESCKPGFVVAEIWNSLSYNGDGKPAANQDQG 249

Query: 387 RQRIINWIDSTGQLSSAFDFTTKGILQEAVKGQLWRLRDCQGKPPGVMGWWPSRAVTFLD 446
           RQ ++NW+++ G  +  FDFTTKG+LQ  V+G+LWRLRD  GK  G++GW P +AVTF+D
Sbjct: 250 RQELVNWVNAVGGPAMTFDFTTKGLLQAGVQGELWRLRDGNGKAAGMIGWLPEKAVTFVD 309

Query: 447 NHDTGSTQAHWPFPANHIMEGYAYILTHPGIPTVFYDHFYDWGDSVHREIVKLIDIRKRQ 506
           NHDTGSTQ  WPFP++ +M+GYAYILTHPG+P +FYDH +DW  ++ +EI  L  IR+R 
Sbjct: 310 NHDTGSTQKLWPFPSDKVMQGYAYILTHPGVPCIFYDHMFDW--NLKQEITALAAIRERN 369

Query: 507 DIYSRSSVKILEARSDLYSAIIGEKLCMKIGD----GSWCPSGTEWTLATSGRRYAVWQK 549
            I + S ++I+ A +D Y A++ EK+ +KIG     G+  PS    T+   G+ Y+VW+K
Sbjct: 370 GINAGSKLRIVVADADAYVAVVDEKVMVKIGTRYDVGNAVPSDFHQTV--HGKDYSVWEK 425

[Close]

BLAST of Spg007196 vs. ExPASy TrEMBL
Match: A0A5A7VG02 (Alpha-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G003440 PE=3 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 1.6e-217
Identity = 355/391 (90.79%), Postives = 366/391 (93.61%), Query Frame = 0


Query: 158 NVNQFEVEAFNWESHKCDWWRNLECKVPDIAKCGFTSVWLPPPTHSIAPEGYLPQNLYSL 217
           N  +   +AFNWESHKCDW  NLECKV DIAK GFTS WLPPPTHSIAP+GYLPQNLYSL
Sbjct: 78  NGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSL 137

Query: 218 NSSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTIQGHGGMHNRFDGIPLPWNESAVT 277
           NSSYGSEDMLKALL KMKQCKVRA+ADIVINHRVGT+ GHGGMHNRFDGI LPW+E AVT
Sbjct: 138 NSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAVT 197

Query: 278 SCSGGLGNRSTGDNFPGVPNIDHSQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGYSAKF 337
           SCSGGLGNRSTGDNF GVPNIDH+QPFVRRDIIAWLKWLRSSVGFQDFRFDFARG+SAKF
Sbjct: 198 SCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGFSAKF 257

Query: 338 VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDRHRQRIINWIDSTGQLSSAFDFTTKGI 397
           VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQD HRQRIINWID TGQLS+AFDFTTKG+
Sbjct: 258 VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFTTKGV 317

Query: 398 LQEAVKGQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYI 457
           LQEAVKGQLWRL DC+GKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYI
Sbjct: 318 LQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYI 377

Query: 458 LTHPGIPTVFYDHFYDWGDSVHREIVKLIDIRKRQDIYSRSSVKILEARSDLYSAIIGEK 517
           LTHPGIPTVFYDHFYDWGDSVH EIVKLIDIRK QDI SRSSVKILEARSDLYSAIIGE 
Sbjct: 378 LTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDLYSAIIGEN 437

Query: 518 LCMKIGDGSWCPSGTEWTLATSGRRYAVWQK 549
           LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 438 LCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 467

[Close]

BLAST of Spg007196 vs. ExPASy TrEMBL
Match: A0A1S3BW59 (Alpha-amylase OS=Cucumis melo OX=3656 GN=LOC103493790 PE=3 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 1.6e-217
Identity = 355/391 (90.79%), Postives = 366/391 (93.61%), Query Frame = 0


Query: 158 NVNQFEVEAFNWESHKCDWWRNLECKVPDIAKCGFTSVWLPPPTHSIAPEGYLPQNLYSL 217
           N  +   +AFNWESHKCDW  NLECKV DIAK GFTS WLPPPTHSIAP+GYLPQNLYSL
Sbjct: 48  NGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSL 107

Query: 218 NSSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTIQGHGGMHNRFDGIPLPWNESAVT 277
           NSSYGSEDMLKALL KMKQCKVRA+ADIVINHRVGT+ GHGGMHNRFDGI LPW+E AVT
Sbjct: 108 NSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAVT 167

Query: 278 SCSGGLGNRSTGDNFPGVPNIDHSQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGYSAKF 337
           SCSGGLGNRSTGDNF GVPNIDH+QPFVRRDIIAWLKWLRSSVGFQDFRFDFARG+SAKF
Sbjct: 168 SCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGFSAKF 227

Query: 338 VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDRHRQRIINWIDSTGQLSSAFDFTTKGI 397
           VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQD HRQRIINWID TGQLS+AFDFTTKG+
Sbjct: 228 VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDGHRQRIINWIDGTGQLSAAFDFTTKGV 287

Query: 398 LQEAVKGQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYI 457
           LQEAVKGQLWRL DC+GKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYI
Sbjct: 288 LQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYI 347

Query: 458 LTHPGIPTVFYDHFYDWGDSVHREIVKLIDIRKRQDIYSRSSVKILEARSDLYSAIIGEK 517
           LTHPGIPTVFYDHFYDWGDSVH EIVKLIDIRK QDI SRSSVKILEARSDLYSAIIGE 
Sbjct: 348 LTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDLYSAIIGEN 407

Query: 518 LCMKIGDGSWCPSGTEWTLATSGRRYAVWQK 549
           LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 408 LCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 437

[Close]

BLAST of Spg007196 vs. ExPASy TrEMBL
Match: A0A5D3D8R2 (Alpha-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00850 PE=3 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 9.0e-216
Identity = 355/395 (89.87%), Postives = 366/395 (92.66%), Query Frame = 0


Query: 158 NVNQFEVEAFNWESHKCDWWRNLECKVPDIAKCGFTSVWLPPPTHSIAPEGYLPQNLYSL 217
           N  +   +AFNWESHKCDW  NLECKV DIAK GFTS WLPPPTHSIAP+GYLPQNLYSL
Sbjct: 78  NGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSL 137

Query: 218 NSSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTIQGHGGMHNRFDGIPLPWNESAVT 277
           NSSYGSEDMLKALL KMKQCKVRA+ADIVINHRVGT+ GHGGMHNRFDGI LPW+E AVT
Sbjct: 138 NSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAVT 197

Query: 278 SCSGGLGNRSTGDNFPGVPNIDHSQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGYSAKF 337
           SCSGGLGNRSTGDNF GVPNIDH+QPFVRRDIIAWLKWLRSSVGFQDFRFDFARG+SAKF
Sbjct: 198 SCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGFSAKF 257

Query: 338 VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ----DRHRQRIINWIDSTGQLSSAFDFT 397
           VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ    D HRQRIINWID TGQLS+AFDFT
Sbjct: 258 VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQALNADGHRQRIINWIDGTGQLSAAFDFT 317

Query: 398 TKGILQEAVKGQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEG 457
           TKG+LQEAVKGQLWRL DC+GKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEG
Sbjct: 318 TKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEG 377

Query: 458 YAYILTHPGIPTVFYDHFYDWGDSVHREIVKLIDIRKRQDIYSRSSVKILEARSDLYSAI 517
           YAYILTHPGIPTVFYDHFYDWGDSVH EIVKLIDIRK QDI SRSSVKILEARSDLYSAI
Sbjct: 378 YAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDLYSAI 437

Query: 518 IGEKLCMKIGDGSWCPSGTEWTLATSGRRYAVWQK 549
           IGE LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 438 IGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 471

[Close]

BLAST of Spg007196 vs. ExPASy TrEMBL
Match: E5GCD3 (Alpha-amylase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 9.0e-216
Identity = 355/395 (89.87%), Postives = 366/395 (92.66%), Query Frame = 0


Query: 158 NVNQFEVEAFNWESHKCDWWRNLECKVPDIAKCGFTSVWLPPPTHSIAPEGYLPQNLYSL 217
           N  +   +AFNWESHKCDW  NLECKV DIAK GFTS WLPPPTHSIAP+GYLPQNLYSL
Sbjct: 23  NGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSL 82

Query: 218 NSSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTIQGHGGMHNRFDGIPLPWNESAVT 277
           NSSYGSEDMLKALL KMKQCKVRA+ADIVINHRVGT+ GHGGMHNRFDGI LPW+E AVT
Sbjct: 83  NSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAVT 142

Query: 278 SCSGGLGNRSTGDNFPGVPNIDHSQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGYSAKF 337
           SCSGGLGNRSTGDNF GVPNIDH+QPFVRRDIIAWLKWLRSSVGFQDFRFDFARG+SAKF
Sbjct: 143 SCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGFSAKF 202

Query: 338 VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ----DRHRQRIINWIDSTGQLSSAFDFT 397
           VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ    D HRQRIINWID TGQLS+AFDFT
Sbjct: 203 VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQALNADGHRQRIINWIDGTGQLSAAFDFT 262

Query: 398 TKGILQEAVKGQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEG 457
           TKG+LQEAVKGQLWRL DC+GKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEG
Sbjct: 263 TKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEG 322

Query: 458 YAYILTHPGIPTVFYDHFYDWGDSVHREIVKLIDIRKRQDIYSRSSVKILEARSDLYSAI 517
           YAYILTHPGIPTVFYDHFYDWGDSVH EIVKLIDIRK QDI SRSSVKILEARSDLYSAI
Sbjct: 323 YAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDLYSAI 382

Query: 518 IGEKLCMKIGDGSWCPSGTEWTLATSGRRYAVWQK 549
           IGE LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 383 IGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 416

[Close]

BLAST of Spg007196 vs. ExPASy TrEMBL
Match: A0A1S4DZE2 (Alpha-amylase OS=Cucumis melo OX=3656 GN=LOC103493790 PE=3 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 2.6e-215
Identity = 355/399 (88.97%), Postives = 366/399 (91.73%), Query Frame = 0


Query: 158 NVNQFEVEAFNWESHKCDWWRNLECKVPDIAKCGFTSVWLPPPTHSIAPEGYLPQNLYSL 217
           N  +   +AFNWESHKCDW  NLECKV DIAK GFTS WLPPPTHSIAP+GYLPQNLYSL
Sbjct: 23  NGREILFQAFNWESHKCDWSGNLECKVHDIAKSGFTSAWLPPPTHSIAPQGYLPQNLYSL 82

Query: 218 NSSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTIQGHGGMHNRFDGIPLPWNESAVT 277
           NSSYGSEDMLKALL KMKQCKVRA+ADIVINHRVGT+ GHGGMHNRFDGI LPW+E AVT
Sbjct: 83  NSSYGSEDMLKALLLKMKQCKVRAMADIVINHRVGTVVGHGGMHNRFDGILLPWDEHAVT 142

Query: 278 SCSGGLGNRSTGDNFPGVPNIDHSQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGYSAKF 337
           SCSGGLGNRSTGDNF GVPNIDH+QPFVRRDIIAWLKWLRSSVGFQDFRFDFARG+SAKF
Sbjct: 143 SCSGGLGNRSTGDNFHGVPNIDHTQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGFSAKF 202

Query: 338 VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ--------DRHRQRIINWIDSTGQLSSA 397
           VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQ        D HRQRIINWID TGQLS+A
Sbjct: 203 VKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQVMITALNADGHRQRIINWIDGTGQLSAA 262

Query: 398 FDFTTKGILQEAVKGQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANH 457
           FDFTTKG+LQEAVKGQLWRL DC+GKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANH
Sbjct: 263 FDFTTKGVLQEAVKGQLWRLCDCRGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANH 322

Query: 458 IMEGYAYILTHPGIPTVFYDHFYDWGDSVHREIVKLIDIRKRQDIYSRSSVKILEARSDL 517
           IMEGYAYILTHPGIPTVFYDHFYDWGDSVH EIVKLIDIRK QDI SRSSVKILEARSDL
Sbjct: 323 IMEGYAYILTHPGIPTVFYDHFYDWGDSVHDEIVKLIDIRKHQDINSRSSVKILEARSDL 382

Query: 518 YSAIIGEKLCMKIGDGSWCPSGTEWTLATSGRRYAVWQK 549
           YSAIIGE LCMKIGDGSWCP GTEWTLAT G RYAVWQK
Sbjct: 383 YSAIIGENLCMKIGDGSWCP-GTEWTLATYGCRYAVWQK 420

[Close]

BLAST of Spg007196 vs. TAIR 10
Match: AT1G76130.1 (alpha-amylase-like 2 )

HSP 1 Score: 706.4 bits (1822), Expect = 1.7e-203
Identity = 310/385 (80.52%), Postives = 357/385 (92.73%), Query Frame = 0


Query: 164 VEAFNWESHKCDWWRNLECKVPDIAKCGFTSVWLPPPTHSIAPEGYLPQNLYSLNSSYGS 223
           ++A+NWESHK DWWRNL+ KVPDIAK GFTS WLPPP+ S+APEGYLPQ+LYSLNS+YGS
Sbjct: 29  LQAYNWESHKYDWWRNLDGKVPDIAKSGFTSAWLPPPSQSLAPEGYLPQDLYSLNSAYGS 88

Query: 224 EDMLKALLQKMKQCKVRAIADIVINHRVGTIQGHGGMHNRFDGIPLPWNESAVTSCSGGL 283
           E +LK+LL+KMKQ KVRA+ADIVINHRVGT +GHGGM+NR+DGI LPW+E AVTSC+GGL
Sbjct: 89  EHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDGISLPWDEHAVTSCTGGL 148

Query: 284 GNRSTGDNFPGVPNIDHSQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGYSAKFVKEYIE 343
           GNRSTGDNF GVPN+DH+Q FVR+DII WL+WLR++VGFQDFRFDFARGYSA +VKEYI 
Sbjct: 149 GNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQDFRFDFARGYSANYVKEYIG 208

Query: 344 AAKPMFSIGEYWDSCNYNGHDLDYNQDRHRQRIINWIDSTGQLSSAFDFTTKGILQEAVK 403
           AAKP+FS+GE WDSCNYNGH LDYNQD HRQRII+WID+TGQ+S+AFDFTTKGILQEAVK
Sbjct: 209 AAKPLFSVGECWDSCNYNGHGLDYNQDSHRQRIISWIDATGQISAAFDFTTKGILQEAVK 268

Query: 404 GQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGYAYILTHPGI 463
           GQ WRL D QGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFP++H+MEGYAYILTHPGI
Sbjct: 269 GQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPSHHVMEGYAYILTHPGI 328

Query: 464 PTVFYDHFYDWGDSVHREIVKLIDIRKRQDIYSRSSVKILEARSDLYSAIIGEKLCMKIG 523
           P VFYDHFYDWG S+H +IVKLIDIR+RQDI+SRS+V++L+A S+LY+AI+GEK+CMK+G
Sbjct: 329 PCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAIVGEKICMKLG 388

Query: 524 DGSWCPSGTEWTLATSGRRYAVWQK 549
           DGSWCPSG +WTLATSG RYAVW K
Sbjct: 389 DGSWCPSGRDWTLATSGHRYAVWHK 413

[Close]

BLAST of Spg007196 vs. TAIR 10
Match: AT1G69830.1 (alpha-amylase-like 3 )

HSP 1 Score: 424.5 bits (1090), Expect = 1.3e-118
Identity = 199/395 (50.38%), Postives = 273/395 (69.11%), Query Frame = 0


Query: 162 FEV--EAFNWESHKCD-WWRNLECKVPDIAKCGFTSVWLPPPTHSIAPEGYLPQNLYSLN 221
           FE+  + FNWES+K   W+  L+ K  ++A  GFT +WLPPPT S++PEGY+P++LY+LN
Sbjct: 495 FEILCQGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYNLN 554

Query: 222 SSYGSEDMLKALLQKMKQCKVRAIADIVINHRVGTIQGHGGMHNRFDGIPLPWNESAVTS 281
           S YG+ D LK  ++K  +  ++ + D V+NHR    +   G+ N F G  L W++ AV +
Sbjct: 555 SRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGG-RLNWDDRAVVA 614

Query: 282 CS---GGLGNRSTGDNFPGVPNIDHSQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGYSA 341
                 G GN+S+GDNF   PNIDHSQ FVR+DI  WL W+   VG+  +R DF RG+  
Sbjct: 615 DDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRLDFVRGFWG 674

Query: 342 KFVKEYIEAAKPMFSIGEYWDSCNYNGHDLDYNQDRHRQRIINWIDSTGQLSSAFDFTTK 401
            +VK+Y++A+KP F++GEYWDS +Y   ++DYNQD HRQRI++WI++T   + AFD TTK
Sbjct: 675 GYVKDYMDASKPYFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAAGAFDVTTK 734

Query: 402 GILQEAV-KGQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIMEGY 461
           GIL  A+ K + WRL D +GKPPGV+GWWPSRAVTF++NHDTGSTQ HW FP    M+GY
Sbjct: 735 GILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPEGKEMQGY 794

Query: 462 AYILTHPGIPTVFYDHFYDWGDSVHREIVKLIDIRKRQDIYSRSSVKILEARSDLYSAII 521
           AYILTHPG P VF+DH +      H EI  L+ +R RQ ++ RS V I ++  D+Y+AII
Sbjct: 795 AYILTHPGTPAVFFDHIF---SDYHSEIAALLSLRNRQKLHCRSEVNIDKSERDVYAAII 854

Query: 522 GEKLCMKIGDGSW-CPSGTE-WTLATSGRRYAVWQ 548
            EK+ MKIG G +  P+G++ W++A  GR Y VW+
Sbjct: 855 DEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWE 885

[Close]

BLAST of Spg007196 vs. TAIR 10
Match: AT4G25000.1 (alpha-amylase-like )

HSP 1 Score: 360.9 bits (925), Expect = 1.8e-99
Identity = 186/400 (46.50%), Postives = 255/400 (63.75%), Query Frame = 0


Query: 165 EAFNWESHKCD--WWRNLECKVPDIAKCGFTSVWLPPPTHSIAPEGYLPQNLYSLNSS-Y 224
           ++FNWES K +  ++ +L   + DIA  G T +WLPPP+ S+APEGYLP  LY LNSS Y
Sbjct: 30  QSFNWESWKKEGGFYNSLHNSIDDIANAGITHLWLPPPSQSVAPEGYLPGKLYDLNSSKY 89

Query: 225 GSEDMLKALLQKMKQCKVRAIADIVINHRVGTIQGHGGMHNRFDG----IPLPWNESAVT 284
           GSE  LK+L++ + Q  ++A+ADIVINHR    +     +  F+G      L W+ S V 
Sbjct: 90  GSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGTSDDRLDWDPSFVC 149

Query: 285 SCS---GGLGNRSTGDNFPGVPNIDHSQPFVRRDIIAWLKWLRSSVGFQDFRFDFARGYS 344
                  G GN  TG +F G P+IDH  P V++++  W+ WL++ +GF  +RFD+ RGY+
Sbjct: 150 RNDPKFPGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKTEIGFHGWRFDYVRGYA 209

Query: 345 AKFVKEYIEAAKPMFSIGEYWDSCNYNGH-DLDYNQDRHRQRIINWIDST-GQLSSAFDF 404
           +   K Y++   P F++GE WD   Y G   LDY+Q+ HR  +  WI+   G + +AFDF
Sbjct: 210 SSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLKQWIEEAGGGVLTAFDF 269

Query: 405 TTKGILQEAVKGQLWRLRDCQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPANHIME 464
           TTKGILQ AVKG+LWRL+D QGKPPG++G  P  AVTF+DNHDT  T   W FP++ ++ 
Sbjct: 270 TTKGILQSAVKGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHDTFRT---WVFPSDKVLL 329

Query: 465 GYAYILTHPGIPTVFYDHFYDWGDSVHREIVKLIDIRKRQDIYSRSSVKILEARSDLYSA 524
           GY YILTHPG P +FY+H+ +WG  +   I KL+ IR +  I S SSV I  A +DLY A
Sbjct: 330 GYVYILTHPGTPCIFYNHYIEWG--LKESISKLVAIRNKNGIGSTSSVTIKAAEADLYLA 389

Query: 525 IIGEKLCMKIGD----GSWCPSGTEWTLATSGRRYAVWQK 549
           +I +K+ MKIG     G+  PS   + LA SG  +AVW+K
Sbjct: 390 MIDDKVIMKIGPKQDVGTLVPS--NFALAYSGLDFAVWEK 422

The following BLAST results are available for this feature:

BLAST of Spg007196 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0064621.1	3.4e-217	90.79	putative alpha-amylase 2 isoform X2 [Cucumis melo var. makuwa]	[more]
XP_008452915.1	3.4e-217	90.79	PREDICTED: probable alpha-amylase 2 isoform X2 [Cucumis melo]	[more]
XP_011654252.1	5.8e-217	90.03	probable alpha-amylase 2 [Cucumis sativus] >XP_031739646.1 probable alpha-amylas...	[more]
ADN34132.1	1.9e-215	89.87	cytosolic alpha-amylase [Cucumis melo subsp. melo]	[more]
TYK19971.1	1.9e-215	89.87	putative alpha-amylase 2 isoform X1 [Cucumis melo var. makuwa]	[more]

BLAST of Spg007196 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LFG1	2.4e-202	80.52	Probable alpha-amylase 2 OS=Arabidopsis thaliana OX=3702 GN=AMY2 PE=2 SV=1	[more]
Q94A41	1.9e-117	50.38	Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1	[more]
P17859	2.3e-107	47.87	Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1	[more]
P27934	1.8e-104	46.75	Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2...	[more]
P27933	2.4e-104	45.71	Alpha-amylase isozyme 3D OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.3 PE=2...	[more]

BLAST of Spg007196 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VG02	1.6e-217	90.79	Alpha-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G003440...	[more]
A0A1S3BW59	1.6e-217	90.79	Alpha-amylase OS=Cucumis melo OX=3656 GN=LOC103493790 PE=3 SV=1	[more]
A0A5D3D8R2	9.0e-216	89.87	Alpha-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00850 ...	[more]
E5GCD3	9.0e-216	89.87	Alpha-amylase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1	[more]
A0A1S4DZE2	2.6e-215	88.97	Alpha-amylase OS=Cucumis melo OX=3656 GN=LOC103493790 PE=3 SV=1	[more]

BLAST of Spg007196 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G76130.1	1.7e-203	80.52	alpha-amylase-like 2	[more]
AT1G69830.1	1.3e-118	50.38	alpha-amylase-like 3	[more]
AT4G25000.1	1.8e-99	46.50	alpha-amylase-like	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012850	Alpha-amylase, C-terminal beta-sheet	SMART	SM00810	alpha-amyl_c2	coord: 490..548 e-value: 8.1E-29 score: 111.7
IPR012850	Alpha-amylase, C-terminal beta-sheet	PFAM	PF07821	Alpha-amyl_C2	coord: 491..547 e-value: 1.0E-21 score: 76.9
IPR006047	Glycosyl hydrolase, family 13, catalytic domain	SMART	SM00642	aamy	coord: 161..501 e-value: 9.1E-28 score: 108.2
IPR006047	Glycosyl hydrolase, family 13, catalytic domain	PFAM	PF00128	Alpha-amylase	coord: 186..250 e-value: 7.7E-10 score: 38.8
IPR013780	Glycosyl hydrolase, all-beta	GENE3D	2.60.40.1180		coord: 494..548 e-value: 1.7E-18 score: 68.4
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 164..493 e-value: 1.2E-119 score: 401.8
None	No IPR available	PIRSR	PIRSR001021-2	PIRSR001021-2	coord: 297..493 e-value: 1.7E-18 score: 64.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..19
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
None	No IPR available	PANTHER	PTHR43447:SF18	ALPHA-AMYLASE 2-RELATED	coord: 157..548
None	No IPR available	PANTHER	PTHR43447	ALPHA-AMYLASE	coord: 157..548
None	No IPR available	CDD	cd11314	AmyAc_arch_bac_plant_AmyA	coord: 166..500 e-value: 4.77844E-168 score: 476.328
None	No IPR available	SUPERFAMILY	51011	Glycosyl hydrolase domain	coord: 494..548
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 169..479

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg007196.1	Spg007196.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004556	alpha-amylase activity
molecular_function	GO:0103025	alpha-amylase activity (releasing maltohexaose)
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0003824	catalytic activity