Spg006339 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg006339 vs. NCBI nr
Match: KAG6589075.1 (Brefeldin A-inhibited guanine nucleotide-exchange protein 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 665.6 bits (1716), Expect = 2.4e-187
Identity = 335/364 (92.03%), Postives = 347/364 (95.33%), Query Frame = 0

Query: 1   MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSIGEAALDPASP 60
           MSVSQ LGG SRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSI EA+LDPASP
Sbjct: 1   MSVSQALGGKSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSISEASLDPASP 60

Query: 61  LAGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGAS 120
           +AGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGAS
Sbjct: 61  VAGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGAS 120

Query: 121 SIVYKIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGL 180
           SIVY+IVESVCKSGGLGDEGIEL VLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGL
Sbjct: 121 SIVYRIVESVCKSGGLGDEGIELIVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGL 180

Query: 181 SGTNQICAKSVLGQIMVIVFSRVEEDTMDVPTRIISVSELLEFTDKNLNEGNSIYFCQNF 240
           SGTNQICAKSVLGQIM+I+FSR+EED+MDVP  IISV+ELLEFTDKNLNEGNSIYFCQNF
Sbjct: 181 SGTNQICAKSVLGQIMIIIFSRMEEDSMDVPASIISVNELLEFTDKNLNEGNSIYFCQNF 240

Query: 241 INEVMDASEGIAEKKLYSFSAQLQNGNASPLKPDNKGESDVGETEDGAESGGCSKIRADG 300
           INEVMDASEG+A+KKL+S   QLQNGNASPLK DNKGESDVGETED AESGGCSKIR DG
Sbjct: 241 INEVMDASEGVADKKLFSSWGQLQNGNASPLKADNKGESDVGETEDVAESGGCSKIREDG 300

Query: 301 FHLFKNLCKLSMKFSSPEHPDDQILLRGKILSLELLKVVMDNAGPVWRSNERQAFHSPSI 360
           FHLFKNLCKLSMKFSSPEHPDDQILLRGK LSLELLKV+MD AGPVWR NERQAFHS SI
Sbjct: 301 FHLFKNLCKLSMKFSSPEHPDDQILLRGKTLSLELLKVIMDKAGPVWRLNERQAFHSSSI 360

Query: 361 ILEL 365
           IL L
Sbjct: 361 ILVL 364

BLAST of Spg006339 vs. NCBI nr
Match: KAG7022782.1 (Brefeldin A-inhibited guanine nucleotide-exchange protein 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 664.5 bits (1713), Expect = 5.4e-187
Identity = 334/362 (92.27%), Postives = 346/362 (95.58%), Query Frame = 0

Query: 1   MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSIGEAALDPASP 60
           MSVSQ LGG SRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSI EA+LDPASP
Sbjct: 1   MSVSQALGGKSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSISEASLDPASP 60

Query: 61  LAGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGAS 120
           +AGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGAS
Sbjct: 61  VAGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGAS 120

Query: 121 SIVYKIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGL 180
           SIVY+IVESVCKSGGLGDEGIEL VLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGL
Sbjct: 121 SIVYRIVESVCKSGGLGDEGIELIVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGL 180

Query: 181 SGTNQICAKSVLGQIMVIVFSRVEEDTMDVPTRIISVSELLEFTDKNLNEGNSIYFCQNF 240
           SGTNQICAKSVLGQIM+I+FSR+EED+MDVP  IISV+ELLEFTDKNLNEGNSIYFCQNF
Sbjct: 181 SGTNQICAKSVLGQIMIIIFSRMEEDSMDVPASIISVNELLEFTDKNLNEGNSIYFCQNF 240

Query: 241 INEVMDASEGIAEKKLYSFSAQLQNGNASPLKPDNKGESDVGETEDGAESGGCSKIRADG 300
           INEVMDASEG+A+KKL+S   QLQNGNASPLK DNKGESDVGETED AESGGCSKIR DG
Sbjct: 241 INEVMDASEGVADKKLFSSWGQLQNGNASPLKADNKGESDVGETEDVAESGGCSKIREDG 300

Query: 301 FHLFKNLCKLSMKFSSPEHPDDQILLRGKILSLELLKVVMDNAGPVWRSNERQAFHSPSI 360
           FHLFKNLCKLSMKFSSPEHPDDQILLRGK LSLELLKV+MD AGPVWR NERQAFHS SI
Sbjct: 301 FHLFKNLCKLSMKFSSPEHPDDQILLRGKTLSLELLKVIMDKAGPVWRLNERQAFHSSSI 360

Query: 361 IL 363
           IL
Sbjct: 361 IL 362

BLAST of Spg006339 vs. NCBI nr
Match: XP_022989285.1 (brefeldin A-inhibited guanine nucleotide-exchange protein 1-like [Cucurbita maxima])

HSP 1 Score: 647.1 bits (1668), Expect = 8.9e-182
Identity = 324/352 (92.05%), Postives = 338/352 (96.02%), Query Frame = 0

Query: 1   MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSIGEAALDPASP 60
           MSVSQ LGG SRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSI EA+LDPASP
Sbjct: 1   MSVSQALGGKSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSISEASLDPASP 60

Query: 61  LAGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGAS 120
           ++GLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGAS
Sbjct: 61  ISGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGAS 120

Query: 121 SIVYKIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGL 180
           SIV++IVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGL
Sbjct: 121 SIVFRIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGL 180

Query: 181 SGTNQICAKSVLGQIMVIVFSRVEEDTMDVPTRIISVSELLEFTDKNLNEGNSIYFCQNF 240
           SGTNQICAKSVLGQIM+I+FSR+EED+MDVP  IISV+ELLEFTDKNLNEGNSIYFCQNF
Sbjct: 181 SGTNQICAKSVLGQIMIIIFSRMEEDSMDVPASIISVNELLEFTDKNLNEGNSIYFCQNF 240

Query: 241 INEVMDASEGIAEKKLYSFSAQLQNGNASPLKPDNKGESDVGETEDGAESGGCSKIRADG 300
           INEVMDASEG+A+KKL+S   QLQNGNASPLK DNKGESDVGETED AESGGCSKIR DG
Sbjct: 241 INEVMDASEGVADKKLFSSWGQLQNGNASPLKADNKGESDVGETEDVAESGGCSKIREDG 300

Query: 301 FHLFKNLCKLSMKFSSPEHPDDQILLRGKILSLELLKVVMDNAGPVWRSNER 353
           FHLFKNLCKLSMKFSSPEHPDDQILLRGK LSLELLKV+MD AGPVWR NER
Sbjct: 301 FHLFKNLCKLSMKFSSPEHPDDQILLRGKTLSLELLKVIMDKAGPVWRLNER 352

BLAST of Spg006339 vs. NCBI nr
Match: KAG6589066.1 (Brefeldin A-inhibited guanine nucleotide-exchange protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 647.1 bits (1668), Expect = 8.9e-182
Identity = 325/352 (92.33%), Postives = 337/352 (95.74%), Query Frame = 0

Query: 1   MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSIGEAALDPASP 60
           MSVSQ LGG SRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSI EA+LDPASP
Sbjct: 1   MSVSQALGGKSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSISEASLDPASP 60

Query: 61  LAGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGAS 120
           +AGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGAS
Sbjct: 61  VAGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGAS 120

Query: 121 SIVYKIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGL 180
           SIVY+IVESVCKSGGLGDEGIEL VLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGL
Sbjct: 121 SIVYRIVESVCKSGGLGDEGIELIVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGL 180

Query: 181 SGTNQICAKSVLGQIMVIVFSRVEEDTMDVPTRIISVSELLEFTDKNLNEGNSIYFCQNF 240
           SGTNQICAKSVLGQIM+I+FSR+EED+MDVP  IISV+ELLEFTDKNLNEGNSIYFCQNF
Sbjct: 181 SGTNQICAKSVLGQIMIIIFSRMEEDSMDVPASIISVNELLEFTDKNLNEGNSIYFCQNF 240

Query: 241 INEVMDASEGIAEKKLYSFSAQLQNGNASPLKPDNKGESDVGETEDGAESGGCSKIRADG 300
           INEVMDASEG+A+KKL+S   QLQNGNASPLK DNKGESDVGETED AESGGCSKIR DG
Sbjct: 241 INEVMDASEGVADKKLFSSWGQLQNGNASPLKADNKGESDVGETEDVAESGGCSKIREDG 300

Query: 301 FHLFKNLCKLSMKFSSPEHPDDQILLRGKILSLELLKVVMDNAGPVWRSNER 353
           FHLFKNLCKLSMKFSSPEHPDDQILLRGK LSLELLKV+MD AGPVWR NER
Sbjct: 301 FHLFKNLCKLSMKFSSPEHPDDQILLRGKTLSLELLKVIMDKAGPVWRLNER 352

BLAST of Spg006339 vs. NCBI nr
Match: XP_022136256.1 (brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1 [Momordica charantia])

HSP 1 Score: 646.0 bits (1665), Expect = 2.0e-181
Identity = 326/352 (92.61%), Postives = 337/352 (95.74%), Query Frame = 0

Query: 1   MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSIGEAALDPASP 60
           MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCK VLDKLDS+GEA LDPAS 
Sbjct: 1   MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKLVLDKLDSLGEAPLDPASG 60

Query: 61  LAGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGAS 120
           LAGLSP DAEFVLQPLLLALDAAYVKVAEPALEC+FKLFSRGLFRGEIERP+ EANS  S
Sbjct: 61  LAGLSPTDAEFVLQPLLLALDAAYVKVAEPALECLFKLFSRGLFRGEIERPESEANSSPS 120

Query: 121 SIVYKIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGL 180
           +IVYKIVESVCKSGGLGDEGIELT LRVLLSAVR PCVLIRGDCLVNVVRTCYNVYLGGL
Sbjct: 121 TIVYKIVESVCKSGGLGDEGIELTSLRVLLSAVRSPCVLIRGDCLVNVVRTCYNVYLGGL 180

Query: 181 SGTNQICAKSVLGQIMVIVFSRVEEDTMDVPTRIISVSELLEFTDKNLNEGNSIYFCQNF 240
           +GTNQICAKSVL QIMV+VFSRVEED+MD P RIISVSELLEFTDKNLNEGNSIYFCQNF
Sbjct: 181 TGTNQICAKSVLAQIMVVVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQNF 240

Query: 241 INEVMDASEGIAEKKLYSFSAQLQNGNASPLKPDNKGESDVGETEDGAESGGCSKIRADG 300
           +NEVMDASEGIAEKKLYSFS+QLQNGNASPLK DNKGESDVGETEDGAESGGCSKIR DG
Sbjct: 241 VNEVMDASEGIAEKKLYSFSSQLQNGNASPLKVDNKGESDVGETEDGAESGGCSKIREDG 300

Query: 301 FHLFKNLCKLSMKFSSPEHPDDQILLRGKILSLELLKVVMDNAGPVWRSNER 353
           FHLFKNLCKLSMKFSS EHPDDQILLRGK+LSLELLKVVMDNAGPVWRS+ER
Sbjct: 301 FHLFKNLCKLSMKFSSQEHPDDQILLRGKVLSLELLKVVMDNAGPVWRSHER 352

BLAST of Spg006339 vs. ExPASy Swiss-Prot
Match: F4JN05 (Brefeldin A-inhibited guanine nucleotide-exchange protein 4 OS=Arabidopsis thaliana OX=3702 GN=BIG4 PE=3 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 1.3e-111
Identity = 207/353 (58.64%), Postives = 268/353 (75.92%), Query Frame = 0

Query: 1   MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSIGEAALDPASP 60
           MS SQTLGG +RCGR IGPSLDKI+KNAAWRKH++LVSSCKSVLDKL+S+ +   DP+S 
Sbjct: 1   MSTSQTLGGATRCGRIIGPSLDKIIKNAAWRKHTYLVSSCKSVLDKLESLPDDFHDPSSV 60

Query: 61  LAGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGAS 120
           ++GL+ +DA+ VLQP LL+L+ AY KV EP+L+C FKLFS  + RGEI+      +S   
Sbjct: 61  VSGLAASDADSVLQPFLLSLETAYSKVVEPSLDCAFKLFSLSILRGEIQ------SSKQD 120

Query: 121 SIVYKIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGL 180
           SI++K+V +V K G + +E I+L VLRVLL+AVR PC+LIRGDCL++VV+TCYN+YLGGL
Sbjct: 121 SILFKLVNAVSKVGAIAEEPIQLAVLRVLLAAVRSPCILIRGDCLLHVVKTCYNIYLGGL 180

Query: 181 SGTNQICAKSVLGQIMVIVFSRVEEDTMDVPTRIISVSELLEFTDKNLNEGNSIYFCQNF 240
           SGT QICAKSVL Q+M+++F+R EED++DV  + I V+ELL FTDK++NEG+S+YFCQ F
Sbjct: 181 SGTTQICAKSVLAQMMLVIFTRSEEDSLDVSVKTIYVNELLTFTDKSVNEGSSVYFCQGF 240

Query: 241 INEVMDASEGIAEKKLYSFSAQLQNGNA-SPLKPDNKG-ESDVGETEDGAESGGCSKIRA 300
           +NEVM A +G            LQN    + + PD+      V   E  +E+G  SK+R 
Sbjct: 241 VNEVMAAGQGSPLPPPDVIQILLQNPETETVMTPDSPSFRGYVANGEGDSETGDMSKVRQ 300

Query: 301 DGFHLFKNLCKLSMKFSSPEHPDDQILLRGKILSLELLKVVMDNAGPVWRSNE 352
           D F LFKNLCKLSM+FSS E+ DDQI++RGK LSLELLKV++DN G VWR+NE
Sbjct: 301 DAFLLFKNLCKLSMRFSSKENNDDQIMVRGKTLSLELLKVIIDNGGSVWRTNE 347

BLAST of Spg006339 vs. ExPASy Swiss-Prot
Match: F4JSZ5 (Brefeldin A-inhibited guanine nucleotide-exchange protein 1 OS=Arabidopsis thaliana OX=3702 GN=BIG1 PE=2 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 3.6e-109
Identity = 207/352 (58.81%), Postives = 263/352 (74.72%), Query Frame = 0

Query: 1   MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSIGEAALDPASP 60
           MS SQ LGG +RCGR IGPSLDKI+KNAAWRKH+ LVS+CKSVLDKL+++ ++  DP+SP
Sbjct: 1   MSSSQNLGGATRCGRVIGPSLDKIIKNAAWRKHTFLVSACKSVLDKLEALSDSP-DPSSP 60

Query: 61  LAGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGAS 120
           L GL+ +DA+ VLQPLLL+LD  Y KV EPAL+C FKLFS  L RGE+       +S   
Sbjct: 61  LFGLTTSDADAVLQPLLLSLDTGYAKVIEPALDCSFKLFSLSLLRGEV------CSSSPD 120

Query: 121 SIVYKIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGL 180
           S++YK++ ++CK  G+G+E IEL VLRVLL+AVR P +LIRGDCL+++VRTCYNVYLGG 
Sbjct: 121 SLLYKLIHAICKVCGIGEESIELAVLRVLLAAVRSPRILIRGDCLLHLVRTCYNVYLGGF 180

Query: 181 SGTNQICAKSVLGQIMVIVFSRVEEDTMDVPTRIISVSELLEFTDKNLNEGNSIYFCQNF 240
           +GTNQICAKSVL QIM+IVF+R E ++MD   + ++V++LL  TDKN+NEGNS++ CQ F
Sbjct: 181 NGTNQICAKSVLAQIMLIVFTRSEANSMDASLKTVNVNDLLAITDKNVNEGNSVHICQGF 240

Query: 241 INEVMDASEGIAEKKLYSFSAQLQNGNASPLKPDNKGESDVGETEDGAESGGCSKIRADG 300
           IN+V+ A E                 + + ++P  +G S    TED    G  SKIR DG
Sbjct: 241 INDVITAGEAAPPP------------DFALVQPPEEGAS---STED---EGTGSKIREDG 300

Query: 301 FHLFKNLCKLSMKFSSPEHPDDQILLRGKILSLELLKVVMDNAGPVWRSNER 353
           F LFKNLCKLSMKFSS E+ DDQIL+RGK LSLELLKV++DN GP+W S+ER
Sbjct: 301 FLLFKNLCKLSMKFSSQENTDDQILVRGKTLSLELLKVIIDNGGPIWLSDER 327

BLAST of Spg006339 vs. ExPASy Swiss-Prot
Match: Q9LZX8 (Brefeldin A-inhibited guanine nucleotide-exchange protein 2 OS=Arabidopsis thaliana OX=3702 GN=BIG2 PE=2 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 1.9e-57
Identity = 147/398 (36.93%), Postives = 214/398 (53.77%), Query Frame = 0

Query: 11  SRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSI-------GEAALDPASPLAG 70
           SR  R + P+L+KIVKNA+WRKHS L + CK+V+++L+S+         AA D  S  + 
Sbjct: 8   SRLSRVVTPALEKIVKNASWRKHSKLANECKAVIERLNSLQKSPPPSSSAATDSESESSV 67

Query: 71  LSP-----------ADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPD 130
             P           AD+E +  PL+ A      K+ EPA++C+ KL + G  RGE + P 
Sbjct: 68  PGPLNDGGSIEYSLADSELIFSPLINACGTGLAKIIEPAIDCIQKLIAHGYIRGESD-PS 127

Query: 131 GEANSGASSIVYKIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTC 190
           G A    S +++K+++SVCK   LGDE IEL VL+ LLSA+    + I G CL+ VVRTC
Sbjct: 128 GGAE---SLLLFKLIDSVCKCHDLGDESIELPVLKTLLSAINSISLRIHGKCLLLVVRTC 187

Query: 191 YNVYLGGLSGTNQICAKSVLGQIMVIVFSRVEEDTMDVPTRIISVSELLEFTDKNLNEGN 250
           Y++YLG  +  NQ  AK+ L QI+VIVF R+E D+  VP + I V+EL+E  +K+  +G 
Sbjct: 188 YDIYLGSKNVVNQTTAKASLIQILVIVFRRMEADSSTVPIQPIVVAELMEPLEKSDADGT 247

Query: 251 SIYFCQNFINEVMDASEGIAEKKLYSFSAQLQNG--------------NASPLKPD---- 310
              F Q FI ++M   +G+    +    +   +G              N + L       
Sbjct: 248 MTQFVQGFITKIMQDIDGVLNPTMSGSGSGSGSGGQDGAYGTTTVETTNPTDLLDSTDKD 307

Query: 311 ------------------NKGESDVGETE--DGAESGGCSKIRADGFHLFKNLCKLSMKF 353
                              KGE   G+ E  D  E    +K+R D   +F+ LCKLSMK 
Sbjct: 308 MLDAKYWEISMYKSALEGRKGELTDGDAERDDDLEVQIENKLRRDACLVFRALCKLSMKA 367

BLAST of Spg006339 vs. ExPASy Swiss-Prot
Match: Q9LPC5 (Brefeldin A-inhibited guanine nucleotide-exchange protein 3 OS=Arabidopsis thaliana OX=3702 GN=BIG3 PE=1 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 1.0e-55
Identity = 144/390 (36.92%), Postives = 216/390 (55.38%), Query Frame = 0

Query: 11  SRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDS--------IGEAALDPASPL- 70
           SR GR + P+LDK++KNA+WRKHS L   CKSV+++L S          E+      PL 
Sbjct: 8   SRLGRVVIPALDKVIKNASWRKHSKLAHECKSVIERLRSPENSSPVADSESGSSIPGPLH 67

Query: 71  ----AGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANS 130
               A  S A++E +L PL+ A     +K+ +PA++C+ KL + G  RGE +   G    
Sbjct: 68  DGGAAEYSLAESEIILSPLINASSTGVLKIVDPAVDCIQKLIAHGYVRGEADPTGGP--- 127

Query: 131 GASSIVYKIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYL 190
             + ++ K++E++CK   L DEG+EL VL+ LL+AV    + I GD L+ +VRTCY +YL
Sbjct: 128 -EALLLSKLIETICKCHELDDEGLELLVLKTLLTAVTSISLRIHGDSLLQIVRTCYGIYL 187

Query: 191 GGLSGTNQICAKSVLGQIMVIVFSRVEEDTMDVPTRIISVSELLEFTDKNLNEGNSIYFC 250
           G  +  NQ  AK+ L Q+ VIVF R+E D+  VP + I V+EL+E  DK+ ++ ++    
Sbjct: 188 GSRNVVNQATAKASLVQMSVIVFRRMEADSSTVPIQPIVVAELMEPMDKSESDPSTTQSV 247

Query: 251 QNFINEVMDASEGIAE----KKLY-----SFSAQLQNGNASPLK---------------- 310
           Q FI ++M   +G+      K  +     +F   L  G A+P                  
Sbjct: 248 QGFITKIMQDIDGVFNSANAKGTFGGHDGAFETSLP-GTANPTDLLDSTDKDMLDAKYWE 307

Query: 311 --------PDNKGESDVGETE--DGAESGGCSKIRADGFHLFKNLCKLSMKFSSPEHPDD 353
                      KGE   GE E  D +E    +K+R D F +F+ LCKLSMK    E P+ 
Sbjct: 308 ISMYKSALEGRKGELADGEVEKDDDSEVQIGNKLRRDAFLVFRALCKLSMKTPPKEDPE- 367

BLAST of Spg006339 vs. ExPASy Swiss-Prot
Match: Q9Y6D5 (Brefeldin A-inhibited guanine nucleotide-exchange protein 2 OS=Homo sapiens OX=9606 GN=ARFGEF2 PE=1 SV=3)

HSP 1 Score: 100.5 bits (249), Expect = 4.1e-20
Identity = 109/422 (25.83%), Postives = 181/422 (42.89%), Query Frame = 0

Query: 17  IGPSLDKIV--KNAAWRKHSHLVSSCKSVLDKLDS-IGEAALDPAS-PLAGLSPADAEFV 76
           +  +L+KI+  K     +HS L  +C+  LD++ + I +  L  A+ P A    AD  F+
Sbjct: 11  VSRALEKILADKEVKRPQHSQLRRACQVALDEIKAEIEKQRLGTAAPPKANFIEADKYFL 70

Query: 77  LQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGASSIVYKIVESVCK 136
             P  LA  +   +V   +L+C+ KL + G   G    PD  + +    ++ +IVE++C 
Sbjct: 71  --PFELACQSKSPRVVSTSLDCLQKLIAYGHITG--NAPD--SGAPGKRLIDRIVETICS 130

Query: 137 --SGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGLSGTNQICAKS 196
              G   DEG++L +++ LL+AV  P + I    ++  VRTCYN+YL   +  NQ  AK+
Sbjct: 131 CFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILQTVRTCYNIYLASKNLINQTTAKA 190

Query: 197 VLGQIMVIVFSRVEEDTM-------------------------------------DVPT- 256
            L Q++ ++F+R+E   +                                       PT 
Sbjct: 191 TLTQMLNVIFTRMENQVLQEARELEKPIQSKPQSPVIQAAAVSPKFVRLKHSQAQSKPTT 250

Query: 257 ------------RIISVSELLEFTDKNLNEGNSIYFCQNFINEVM-------------DA 316
                       R  S     E  D     G+S+    +   EV+             +A
Sbjct: 251 PEKTDLTNGEHARSDSGKVSTENGDAPRERGSSLSGTDDGAQEVVKDILEDVVTSAIKEA 310

Query: 317 SE--GIAEKKLYSFSAQLQ----------NGNASPLKPDNKGESDVGETEDGAESGGCSK 352
           +E  G+ E +      + Q          N   + +  D +  S     E  A+    + 
Sbjct: 311 AEKHGLTEPERVLGELECQECAIPPGVDENSQTNGIADDRQSLSSADNLESDAQGHQVAA 370

BLAST of Spg006339 vs. ExPASy TrEMBL
Match: A0A6J1JLY0 (brefeldin A-inhibited guanine nucleotide-exchange protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111486404 PE=4 SV=1)

HSP 1 Score: 647.1 bits (1668), Expect = 4.3e-182
Identity = 324/352 (92.05%), Postives = 338/352 (96.02%), Query Frame = 0

Query: 1   MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSIGEAALDPASP 60
           MSVSQ LGG SRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSI EA+LDPASP
Sbjct: 1   MSVSQALGGKSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSISEASLDPASP 60

Query: 61  LAGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGAS 120
           ++GLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGAS
Sbjct: 61  ISGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGAS 120

Query: 121 SIVYKIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGL 180
           SIV++IVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGL
Sbjct: 121 SIVFRIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGL 180

Query: 181 SGTNQICAKSVLGQIMVIVFSRVEEDTMDVPTRIISVSELLEFTDKNLNEGNSIYFCQNF 240
           SGTNQICAKSVLGQIM+I+FSR+EED+MDVP  IISV+ELLEFTDKNLNEGNSIYFCQNF
Sbjct: 181 SGTNQICAKSVLGQIMIIIFSRMEEDSMDVPASIISVNELLEFTDKNLNEGNSIYFCQNF 240

Query: 241 INEVMDASEGIAEKKLYSFSAQLQNGNASPLKPDNKGESDVGETEDGAESGGCSKIRADG 300
           INEVMDASEG+A+KKL+S   QLQNGNASPLK DNKGESDVGETED AESGGCSKIR DG
Sbjct: 241 INEVMDASEGVADKKLFSSWGQLQNGNASPLKADNKGESDVGETEDVAESGGCSKIREDG 300

Query: 301 FHLFKNLCKLSMKFSSPEHPDDQILLRGKILSLELLKVVMDNAGPVWRSNER 353
           FHLFKNLCKLSMKFSSPEHPDDQILLRGK LSLELLKV+MD AGPVWR NER
Sbjct: 301 FHLFKNLCKLSMKFSSPEHPDDQILLRGKTLSLELLKVIMDKAGPVWRLNER 352

BLAST of Spg006339 vs. ExPASy TrEMBL
Match: A0A6J1C309 (brefeldin A-inhibited guanine nucleotide-exchange protein 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007998 PE=4 SV=1)

HSP 1 Score: 646.0 bits (1665), Expect = 9.6e-182
Identity = 326/352 (92.61%), Postives = 337/352 (95.74%), Query Frame = 0

Query: 1   MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSIGEAALDPASP 60
           MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCK VLDKLDS+GEA LDPAS 
Sbjct: 1   MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKLVLDKLDSLGEAPLDPASG 60

Query: 61  LAGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGAS 120
           LAGLSP DAEFVLQPLLLALDAAYVKVAEPALEC+FKLFSRGLFRGEIERP+ EANS  S
Sbjct: 61  LAGLSPTDAEFVLQPLLLALDAAYVKVAEPALECLFKLFSRGLFRGEIERPESEANSSPS 120

Query: 121 SIVYKIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGL 180
           +IVYKIVESVCKSGGLGDEGIELT LRVLLSAVR PCVLIRGDCLVNVVRTCYNVYLGGL
Sbjct: 121 TIVYKIVESVCKSGGLGDEGIELTSLRVLLSAVRSPCVLIRGDCLVNVVRTCYNVYLGGL 180

Query: 181 SGTNQICAKSVLGQIMVIVFSRVEEDTMDVPTRIISVSELLEFTDKNLNEGNSIYFCQNF 240
           +GTNQICAKSVL QIMV+VFSRVEED+MD P RIISVSELLEFTDKNLNEGNSIYFCQNF
Sbjct: 181 TGTNQICAKSVLAQIMVVVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQNF 240

Query: 241 INEVMDASEGIAEKKLYSFSAQLQNGNASPLKPDNKGESDVGETEDGAESGGCSKIRADG 300
           +NEVMDASEGIAEKKLYSFS+QLQNGNASPLK DNKGESDVGETEDGAESGGCSKIR DG
Sbjct: 241 VNEVMDASEGIAEKKLYSFSSQLQNGNASPLKVDNKGESDVGETEDGAESGGCSKIREDG 300

Query: 301 FHLFKNLCKLSMKFSSPEHPDDQILLRGKILSLELLKVVMDNAGPVWRSNER 353
           FHLFKNLCKLSMKFSS EHPDDQILLRGK+LSLELLKVVMDNAGPVWRS+ER
Sbjct: 301 FHLFKNLCKLSMKFSSQEHPDDQILLRGKVLSLELLKVVMDNAGPVWRSHER 352

BLAST of Spg006339 vs. ExPASy TrEMBL
Match: A0A6J1EK91 (brefeldin A-inhibited guanine nucleotide-exchange protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111435118 PE=4 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 1.6e-181
Identity = 324/352 (92.05%), Postives = 336/352 (95.45%), Query Frame = 0

Query: 1   MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSIGEAALDPASP 60
           MSVSQ LGG SRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSI EA+LDPASP
Sbjct: 1   MSVSQALGGKSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSISEASLDPASP 60

Query: 61  LAGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGAS 120
           +AGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGAS
Sbjct: 61  IAGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGAS 120

Query: 121 SIVYKIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGL 180
           SIVY+IVESVCKSGGLGDEGIEL VLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGL
Sbjct: 121 SIVYRIVESVCKSGGLGDEGIELIVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGL 180

Query: 181 SGTNQICAKSVLGQIMVIVFSRVEEDTMDVPTRIISVSELLEFTDKNLNEGNSIYFCQNF 240
           SGTNQICAKSVLGQIM+I+FSR+EED+MDVP  IISV+ELLEFTDKNLNEGNSIYFCQNF
Sbjct: 181 SGTNQICAKSVLGQIMIIIFSRMEEDSMDVPASIISVNELLEFTDKNLNEGNSIYFCQNF 240

Query: 241 INEVMDASEGIAEKKLYSFSAQLQNGNASPLKPDNKGESDVGETEDGAESGGCSKIRADG 300
           INEVMDASEG+A+KKL+S   QLQNGNASPLK DNKGESDVGETED AES GCSKIR DG
Sbjct: 241 INEVMDASEGVADKKLFSSWGQLQNGNASPLKADNKGESDVGETEDVAESSGCSKIREDG 300

Query: 301 FHLFKNLCKLSMKFSSPEHPDDQILLRGKILSLELLKVVMDNAGPVWRSNER 353
           FHLFKNLCKLSMKFSSPEHPDDQILLRGK LSLELLKV+MD AGPVWR NER
Sbjct: 301 FHLFKNLCKLSMKFSSPEHPDDQILLRGKTLSLELLKVIMDKAGPVWRLNER 352

BLAST of Spg006339 vs. ExPASy TrEMBL
Match: A0A0A0K1N6 (SEC7 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G027890 PE=4 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 6.9e-180
Identity = 327/353 (92.63%), Postives = 336/353 (95.18%), Query Frame = 0

Query: 1   MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSIGEAA-LDPAS 60
           MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSI EA   DP S
Sbjct: 1   MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSIAEAVPPDPTS 60

Query: 61  PLAGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGA 120
           PLAGLSPADA+FVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDG+ANS A
Sbjct: 61  PLAGLSPADADFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGDANSNA 120

Query: 121 SSIVYKIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGG 180
           SSIVYKIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGG
Sbjct: 121 SSIVYKIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGG 180

Query: 181 LSGTNQICAKSVLGQIMVIVFSRVEEDTMDVPTRIISVSELLEFTDKNLNEGNSIYFCQN 240
           LSGTNQICAKSVLGQ+MVIVFSRVEED+MD P RIISVSELLEFTDKNLNEGNSIYFCQN
Sbjct: 181 LSGTNQICAKSVLGQVMVIVFSRVEEDSMDAPMRIISVSELLEFTDKNLNEGNSIYFCQN 240

Query: 241 FINEVMDASEGIAEKKLYSFSAQLQNGNASPLKPDNKGESDVGETEDGAESGGCSKIRAD 300
           FINEVMDASEGIA+KKLY FSA+LQNG+ASPLK DNKGESD+GETED      CSKIR D
Sbjct: 241 FINEVMDASEGIADKKLYEFSAKLQNGHASPLKVDNKGESDIGETED-----VCSKIRED 300

Query: 301 GFHLFKNLCKLSMKFSSPEHPDDQILLRGKILSLELLKVVMDNAGPVWRSNER 353
           GFHLFKNLCKLSMKFSSPEHPDDQIL+RGKILSLELLKVVMDNAGPVWRSNER
Sbjct: 301 GFHLFKNLCKLSMKFSSPEHPDDQILIRGKILSLELLKVVMDNAGPVWRSNER 348

BLAST of Spg006339 vs. ExPASy TrEMBL
Match: A0A6J1I124 (brefeldin A-inhibited guanine nucleotide-exchange protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111468096 PE=4 SV=1)

HSP 1 Score: 639.0 bits (1647), Expect = 1.2e-179
Identity = 321/352 (91.19%), Postives = 335/352 (95.17%), Query Frame = 0

Query: 1   MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSIGEAALDPASP 60
           MS+SQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSI EAALDPASP
Sbjct: 1   MSISQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSIKEAALDPASP 60

Query: 61  LAGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGAS 120
           +AGLSPADAEFVLQPLLLALDAAYVKV+EP LECVFKLFSRG+F GEIERPDGEANSGA 
Sbjct: 61  IAGLSPADAEFVLQPLLLALDAAYVKVSEPTLECVFKLFSRGIFCGEIERPDGEANSGAK 120

Query: 121 SIVYKIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGL 180
           SI+YKI+ESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIR DCLV VVR CYNVYLGGL
Sbjct: 121 SIMYKIIESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRRDCLVTVVRNCYNVYLGGL 180

Query: 181 SGTNQICAKSVLGQIMVIVFSRVEEDTMDVPTRIISVSELLEFTDKNLNEGNSIYFCQNF 240
           S TNQICAKSVL QIMVIVFSR EE++MD P RII+VSELLEFTDKNLNEGNS+YFCQNF
Sbjct: 181 SVTNQICAKSVLAQIMVIVFSRAEENSMDAPLRIITVSELLEFTDKNLNEGNSLYFCQNF 240

Query: 241 INEVMDASEGIAEKKLYSFSAQLQNGNASPLKPDNKGESDVGETEDGAESGGCSKIRADG 300
           INE+MDASEGIA+ KLYSFSAQLQNG+ASPLK DNKG+SDVGE EDGAESGGCSKIR DG
Sbjct: 241 INEIMDASEGIADLKLYSFSAQLQNGHASPLKGDNKGDSDVGEVEDGAESGGCSKIRLDG 300

Query: 301 FHLFKNLCKLSMKFSSPEHPDDQILLRGKILSLELLKVVMDNAGPVWRSNER 353
           FHLFKNLCKLSMKFSSPEHPDDQILLRGKILSLELLKVVM NAGPVWRSNER
Sbjct: 301 FHLFKNLCKLSMKFSSPEHPDDQILLRGKILSLELLKVVMGNAGPVWRSNER 352

BLAST of Spg006339 vs. TAIR 10
Match: AT4G35380.1 (SEC7-like guanine nucleotide exchange family protein )

HSP 1 Score: 404.4 bits (1038), Expect = 9.4e-113
Identity = 207/353 (58.64%), Postives = 268/353 (75.92%), Query Frame = 0

Query: 1   MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSIGEAALDPASP 60
           MS SQTLGG +RCGR IGPSLDKI+KNAAWRKH++LVSSCKSVLDKL+S+ +   DP+S 
Sbjct: 1   MSTSQTLGGATRCGRIIGPSLDKIIKNAAWRKHTYLVSSCKSVLDKLESLPDDFHDPSSV 60

Query: 61  LAGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGAS 120
           ++GL+ +DA+ VLQP LL+L+ AY KV EP+L+C FKLFS  + RGEI+      +S   
Sbjct: 61  VSGLAASDADSVLQPFLLSLETAYSKVVEPSLDCAFKLFSLSILRGEIQ------SSKQD 120

Query: 121 SIVYKIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGL 180
           SI++K+V +V K G + +E I+L VLRVLL+AVR PC+LIRGDCL++VV+TCYN+YLGGL
Sbjct: 121 SILFKLVNAVSKVGAIAEEPIQLAVLRVLLAAVRSPCILIRGDCLLHVVKTCYNIYLGGL 180

Query: 181 SGTNQICAKSVLGQIMVIVFSRVEEDTMDVPTRIISVSELLEFTDKNLNEGNSIYFCQNF 240
           SGT QICAKSVL Q+M+++F+R EED++DV  + I V+ELL FTDK++NEG+S+YFCQ F
Sbjct: 181 SGTTQICAKSVLAQMMLVIFTRSEEDSLDVSVKTIYVNELLTFTDKSVNEGSSVYFCQGF 240

Query: 241 INEVMDASEGIAEKKLYSFSAQLQNGNA-SPLKPDNKG-ESDVGETEDGAESGGCSKIRA 300
           +NEVM A +G            LQN    + + PD+      V   E  +E+G  SK+R 
Sbjct: 241 VNEVMAAGQGSPLPPPDVIQILLQNPETETVMTPDSPSFRGYVANGEGDSETGDMSKVRQ 300

Query: 301 DGFHLFKNLCKLSMKFSSPEHPDDQILLRGKILSLELLKVVMDNAGPVWRSNE 352
           D F LFKNLCKLSM+FSS E+ DDQI++RGK LSLELLKV++DN G VWR+NE
Sbjct: 301 DAFLLFKNLCKLSMRFSSKENNDDQIMVRGKTLSLELLKVIIDNGGSVWRTNE 347

BLAST of Spg006339 vs. TAIR 10
Match: AT4G38200.1 (SEC7-like guanine nucleotide exchange family protein )

HSP 1 Score: 396.4 bits (1017), Expect = 2.6e-110
Identity = 207/352 (58.81%), Postives = 263/352 (74.72%), Query Frame = 0

Query: 1   MSVSQTLGGTSRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSIGEAALDPASP 60
           MS SQ LGG +RCGR IGPSLDKI+KNAAWRKH+ LVS+CKSVLDKL+++ ++  DP+SP
Sbjct: 1   MSSSQNLGGATRCGRVIGPSLDKIIKNAAWRKHTFLVSACKSVLDKLEALSDSP-DPSSP 60

Query: 61  LAGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGAS 120
           L GL+ +DA+ VLQPLLL+LD  Y KV EPAL+C FKLFS  L RGE+       +S   
Sbjct: 61  LFGLTTSDADAVLQPLLLSLDTGYAKVIEPALDCSFKLFSLSLLRGEV------CSSSPD 120

Query: 121 SIVYKIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGL 180
           S++YK++ ++CK  G+G+E IEL VLRVLL+AVR P +LIRGDCL+++VRTCYNVYLGG 
Sbjct: 121 SLLYKLIHAICKVCGIGEESIELAVLRVLLAAVRSPRILIRGDCLLHLVRTCYNVYLGGF 180

Query: 181 SGTNQICAKSVLGQIMVIVFSRVEEDTMDVPTRIISVSELLEFTDKNLNEGNSIYFCQNF 240
           +GTNQICAKSVL QIM+IVF+R E ++MD   + ++V++LL  TDKN+NEGNS++ CQ F
Sbjct: 181 NGTNQICAKSVLAQIMLIVFTRSEANSMDASLKTVNVNDLLAITDKNVNEGNSVHICQGF 240

Query: 241 INEVMDASEGIAEKKLYSFSAQLQNGNASPLKPDNKGESDVGETEDGAESGGCSKIRADG 300
           IN+V+ A E                 + + ++P  +G S    TED    G  SKIR DG
Sbjct: 241 INDVITAGEAAPPP------------DFALVQPPEEGAS---STED---EGTGSKIREDG 300

Query: 301 FHLFKNLCKLSMKFSSPEHPDDQILLRGKILSLELLKVVMDNAGPVWRSNER 353
           F LFKNLCKLSMKFSS E+ DDQIL+RGK LSLELLKV++DN GP+W S+ER
Sbjct: 301 FLLFKNLCKLSMKFSSQENTDDQILVRGKTLSLELLKVIIDNGGPIWLSDER 327

BLAST of Spg006339 vs. TAIR 10
Match: AT3G60860.1 (SEC7-like guanine nucleotide exchange family protein )

HSP 1 Score: 224.6 bits (571), Expect = 1.3e-58
Identity = 147/398 (36.93%), Postives = 214/398 (53.77%), Query Frame = 0

Query: 11  SRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDSI-------GEAALDPASPLAG 70
           SR  R + P+L+KIVKNA+WRKHS L + CK+V+++L+S+         AA D  S  + 
Sbjct: 8   SRLSRVVTPALEKIVKNASWRKHSKLANECKAVIERLNSLQKSPPPSSSAATDSESESSV 67

Query: 71  LSP-----------ADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPD 130
             P           AD+E +  PL+ A      K+ EPA++C+ KL + G  RGE + P 
Sbjct: 68  PGPLNDGGSIEYSLADSELIFSPLINACGTGLAKIIEPAIDCIQKLIAHGYIRGESD-PS 127

Query: 131 GEANSGASSIVYKIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTC 190
           G A    S +++K+++SVCK   LGDE IEL VL+ LLSA+    + I G CL+ VVRTC
Sbjct: 128 GGAE---SLLLFKLIDSVCKCHDLGDESIELPVLKTLLSAINSISLRIHGKCLLLVVRTC 187

Query: 191 YNVYLGGLSGTNQICAKSVLGQIMVIVFSRVEEDTMDVPTRIISVSELLEFTDKNLNEGN 250
           Y++YLG  +  NQ  AK+ L QI+VIVF R+E D+  VP + I V+EL+E  +K+  +G 
Sbjct: 188 YDIYLGSKNVVNQTTAKASLIQILVIVFRRMEADSSTVPIQPIVVAELMEPLEKSDADGT 247

Query: 251 SIYFCQNFINEVMDASEGIAEKKLYSFSAQLQNG--------------NASPLKPD---- 310
              F Q FI ++M   +G+    +    +   +G              N + L       
Sbjct: 248 MTQFVQGFITKIMQDIDGVLNPTMSGSGSGSGSGGQDGAYGTTTVETTNPTDLLDSTDKD 307

Query: 311 ------------------NKGESDVGETE--DGAESGGCSKIRADGFHLFKNLCKLSMKF 353
                              KGE   G+ E  D  E    +K+R D   +F+ LCKLSMK 
Sbjct: 308 MLDAKYWEISMYKSALEGRKGELTDGDAERDDDLEVQIENKLRRDACLVFRALCKLSMKA 367

BLAST of Spg006339 vs. TAIR 10
Match: AT1G01960.1 (SEC7-like guanine nucleotide exchange family protein )

HSP 1 Score: 218.8 bits (556), Expect = 7.3e-57
Identity = 144/390 (36.92%), Postives = 216/390 (55.38%), Query Frame = 0

Query: 11  SRCGRAIGPSLDKIVKNAAWRKHSHLVSSCKSVLDKLDS--------IGEAALDPASPL- 70
           SR GR + P+LDK++KNA+WRKHS L   CKSV+++L S          E+      PL 
Sbjct: 8   SRLGRVVIPALDKVIKNASWRKHSKLAHECKSVIERLRSPENSSPVADSESGSSIPGPLH 67

Query: 71  ----AGLSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANS 130
               A  S A++E +L PL+ A     +K+ +PA++C+ KL + G  RGE +   G    
Sbjct: 68  DGGAAEYSLAESEIILSPLINASSTGVLKIVDPAVDCIQKLIAHGYVRGEADPTGGP--- 127

Query: 131 GASSIVYKIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYL 190
             + ++ K++E++CK   L DEG+EL VL+ LL+AV    + I GD L+ +VRTCY +YL
Sbjct: 128 -EALLLSKLIETICKCHELDDEGLELLVLKTLLTAVTSISLRIHGDSLLQIVRTCYGIYL 187

Query: 191 GGLSGTNQICAKSVLGQIMVIVFSRVEEDTMDVPTRIISVSELLEFTDKNLNEGNSIYFC 250
           G  +  NQ  AK+ L Q+ VIVF R+E D+  VP + I V+EL+E  DK+ ++ ++    
Sbjct: 188 GSRNVVNQATAKASLVQMSVIVFRRMEADSSTVPIQPIVVAELMEPMDKSESDPSTTQSV 247

Query: 251 QNFINEVMDASEGIAE----KKLY-----SFSAQLQNGNASPLK---------------- 310
           Q FI ++M   +G+      K  +     +F   L  G A+P                  
Sbjct: 248 QGFITKIMQDIDGVFNSANAKGTFGGHDGAFETSLP-GTANPTDLLDSTDKDMLDAKYWE 307

Query: 311 --------PDNKGESDVGETE--DGAESGGCSKIRADGFHLFKNLCKLSMKFSSPEHPDD 353
                      KGE   GE E  D +E    +K+R D F +F+ LCKLSMK    E P+ 
Sbjct: 308 ISMYKSALEGRKGELADGEVEKDDDSEVQIGNKLRRDAFLVFRALCKLSMKTPPKEDPE- 367

BLAST of Spg006339 vs. TAIR 10
Match: AT3G43300.1 (HOPM interactor 7 )

HSP 1 Score: 91.3 bits (225), Expect = 1.8e-18
Identity = 85/305 (27.87%), Postives = 137/305 (44.92%), Query Frame = 0

Query: 64  LSPADAEFVLQPLLLALDAAYVKVAEPALECVFKLFSRGLFRGEIERPDGEANSGASSIV 123
           L  A+ E VL+PL LA +   +K+ + AL+C+ KL +     G+    DG  NS   + +
Sbjct: 99  LGGAEVELVLKPLRLAFETKNLKIFDAALDCLHKLIAYDHLEGD-PGLDGGKNSAPFTDI 158

Query: 124 YKIVESVCKSGGLGDEGIELTVLRVLLSAVRCPCVLIRGDCLVNVVRTCYNVYLGGLSGT 183
             +V S C      D  + L VL+VLL+AV      + G+ L+ V+R CYN+ L   S  
Sbjct: 159 LNMVCS-CVDNSSPDSTV-LQVLKVLLTAVASGKFKVHGEPLLGVIRVCYNIALNSKSPI 218

Query: 184 NQICAKSVLGQIMVIVFSRVEEDTMDVPTRIISVSELLEFTDKNLNEGNSIYFCQNFINE 243
           NQ  +K++L Q++ IVF R+E D +   + +     +   T    NE            E
Sbjct: 219 NQATSKAMLTQMISIVFRRMETDIVSASSTVSQEEHVSGDTSSPKNE------------E 278

Query: 244 VMDASEGIAEKKLYSFSAQLQNGNASPLK--------PDNKG----------ESDVGETE 303
           +  A E   E  L     Q ++   + ++         D KG            D  + +
Sbjct: 279 ITAADENEKEMTLGDALTQAKDTTLASVEELHTLVGGADIKGLEAALDKAVHLEDGKKIK 338

Query: 304 DGAESGGCSKIRADGFHLFKNLCKLSMKFSSPEHPDDQILLRGKILSLELLKVVMDNAGP 351
            G E    S  + D   +F+ LCK+ MK  S     D++  + +ILSLELL+ +++    
Sbjct: 339 RGIELESMSIGQRDALLVFRTLCKMGMKEDS-----DEVTTKTRILSLELLQGMLEGVSH 383