Spg006200 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg006200
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionLINE-1 retrotransposable element ORF2 protein
Locationscaffold4: 4745680 .. 4746463 (+)
RNA-Seq ExpressionSpg006200
SyntenySpg006200
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCTTGGTCAGGCTGGAGAACTTTGAACCTCTTGTGGATGCAACCTCCATACTCGCTCAAATCGCCAAGGATGCCGACGCCAAATTCACTCCGAGGATGTTCTTGATCATCGCCTCGCACAGTTCCCCTCGGTTCGTCGCGACGCTGCAGATGTCGCGTCGATTCTTTACGAACTATTTGATCGATCATAATCACGTTTCGAGGATCTCCCTTGAATCCTTCCATGATGCTATGTTGGATGGAGGGAGTTACTCTTCAATGACAATGCATCTTCTTGAAAACATAAATCAAATGGTCCTTAGGTTTGATGCTTCAGGTGAGTATATAAATAATACACATTTCTTCATAACAATGAATCTTGGTTCTTATTTCTTTGATTGATTATATTCGTTTGGTTTAATTTCAAGAACACAGTTAATTCAATAATTTGTTGATATCCAATCTTAGGGCATGCGCCACCGTTGCATCATGAATTGACATTGTCGCCTTCGCAAGAAGACGATATATTGGGTCAAGTTACATATGGGAAATATTTCACAGTTCATTCTAAAGAATTAAGACGTATTATAAAAGAATTGCCACTCTTCCAAGCGGATTCAGGTATTGTTAAACTACTAGAGCTATTCCACTCCCTGTTTAACCGGGAGAATGAGTTGACTAGTTAAAATATATCTCTTGTTTGTTTGTCACAGTTTGTGTTACGGTAACGAGTTCACGAATCAAGTTCTCTATTGCATCTAAGGAGATTATTCTTACGAAAGAGGTACGGTTCATTTGCTAA

mRNA sequence

ATGTTCTTGGTCAGGCTGGAGAACTTTGAACCTCTTGTGGATGCAACCTCCATACTCGCTCAAATCGCCAAGGATGCCGACGCCAAATTCACTCCGAGGATGTTCTTGATCATCGCCTCGCACAGTTCCCCTCGGTTCGTCGCGACGCTGCAGATGTCGCGTCGATTCTTTACGAACTATTTGATCGATCATAATCACGTTTCGAGGATCTCCCTTGAATCCTTCCATGATGCTATGTTGGATGGAGGGAGTTACTCTTCAATGACAATGCATCTTCTTGAAAACATAAATCAAATGGTCCTTAGGTTTGATGCTTCAGGGCATGCGCCACCGTTGCATCATGAATTGACATTGTCGCCTTCGCAAGAAGACGATATATTGGGTCAAGTTACATATGGGAAATATTTCACAGTTCATTCTAAAGAATTAAGACGTATTATAAAAGAATTGCCACTCTTCCAAGCGGATTCAGTTTGTGTTACGGTAACGAGTTCACGAATCAAGTTCTCTATTGCATCTAAGGAGATTATTCTTACGAAAGAGGTACGGTTCATTTGCTAA

Coding sequence (CDS)

ATGTTCTTGGTCAGGCTGGAGAACTTTGAACCTCTTGTGGATGCAACCTCCATACTCGCTCAAATCGCCAAGGATGCCGACGCCAAATTCACTCCGAGGATGTTCTTGATCATCGCCTCGCACAGTTCCCCTCGGTTCGTCGCGACGCTGCAGATGTCGCGTCGATTCTTTACGAACTATTTGATCGATCATAATCACGTTTCGAGGATCTCCCTTGAATCCTTCCATGATGCTATGTTGGATGGAGGGAGTTACTCTTCAATGACAATGCATCTTCTTGAAAACATAAATCAAATGGTCCTTAGGTTTGATGCTTCAGGGCATGCGCCACCGTTGCATCATGAATTGACATTGTCGCCTTCGCAAGAAGACGATATATTGGGTCAAGTTACATATGGGAAATATTTCACAGTTCATTCTAAAGAATTAAGACGTATTATAAAAGAATTGCCACTCTTCCAAGCGGATTCAGTTTGTGTTACGGTAACGAGTTCACGAATCAAGTTCTCTATTGCATCTAAGGAGATTATTCTTACGAAAGAGGTACGGTTCATTTGCTAA

Protein sequence

MFLVRLENFEPLVDATSILAQIAKDADAKFTPRMFLIIASHSSPRFVATLQMSRRFFTNYLIDHNHVSRISLESFHDAMLDGGSYSSMTMHLLENINQMVLRFDASGHAPPLHHELTLSPSQEDDILGQVTYGKYFTVHSKELRRIIKELPLFQADSVCVTVTSSRIKFSIASKEIILTKEVRFIC
Homology
BLAST of Spg006200 vs. NCBI nr
Match: XP_008458682.1 (PREDICTED: uncharacterized protein LOC103498010 [Cucumis melo])

HSP 1 Score: 255.4 bits (651), Expect = 3.9e-64
Identity = 129/182 (70.88%), Postives = 152/182 (83.52%), Query Frame = 0

Query: 1   MFLVRLENFEPLVDATSILAQIAKDADAKFTPRMFLIIASHSSPRFVATLQMSRRFFTNY 60
           MFLVRLE FEPL+DATS+LAQ+AKDAD KFTP M +II S+ SP+FVATLQ+SRR FTN+
Sbjct: 1   MFLVRLEQFEPLIDATSLLAQVAKDADVKFTPLMLMIIVSNRSPQFVATLQLSRRLFTNF 60

Query: 61  LIDHNHVSRISLESFHDAMLDGGSYSSMTMHLLENINQMVLRFDASGH-APPLHHELTLS 120
            +DHN  S++SL+ FHDAMLDGGS+SSMT+HLL+  NQMVLRF+   H  PPLHHEL LS
Sbjct: 61  SVDHNKSSKVSLQPFHDAMLDGGSFSSMTIHLLDTTNQMVLRFETPSHDVPPLHHELALS 120

Query: 121 PSQEDDILGQVTYGKYFTVHSKELRRIIKELPLFQADSVCVTVTSSRIKFSIASKEIILT 180
           P Q ++ LGQV YG +FTV S+ELRRIIKELPLF  D+V VTVT S++KFSI SKEIILT
Sbjct: 121 PPQAEN-LGQVEYGNFFTVTSRELRRIIKELPLFHQDTVSVTVTGSQVKFSIQSKEIILT 180

Query: 181 KE 182
           KE
Sbjct: 181 KE 181

BLAST of Spg006200 vs. NCBI nr
Match: XP_016903187.1 (PREDICTED: uncharacterized protein LOC103502263 [Cucumis melo])

HSP 1 Score: 247.7 bits (631), Expect = 8.0e-62
Identity = 122/187 (65.24%), Postives = 156/187 (83.42%), Query Frame = 0

Query: 1   MFLVRLENFEPLVDATSILAQIAKDADAKFTPRMFLIIASHSSPRFVATLQMSRRFFTNY 60
           MFLVRL++F+PL DATS LAQIA++AD KFTP  F IIAS+ SPRFVA LQM+   F NY
Sbjct: 3   MFLVRLKDFDPLFDATSRLAQIAREADIKFTPLFFSIIASNRSPRFVAYLQMTHHCFINY 62

Query: 61  LIDHNHVSRISLESFHDAMLDGGSYSSMTMHLLENINQMVLRFDASGHAPPLHHELTLSP 120
            +D++H SRISLESFHDA+LDGG+  SMT+HLL NI Q++LRF++S HAP +HHEL+L+P
Sbjct: 63  KVDNDHTSRISLESFHDALLDGGASPSMTIHLLANIKQLILRFESSSHAPKVHHELSLTP 122

Query: 121 SQEDDILGQVTYGKYFTVHSKELRRIIKELPLFQADSVCVTVTSSRIKFSIASKEIILTK 180
           SQE+D LG+V Y K+F++ SK+LRR+I+ LP+F  DS+CVT T S++KFSIASKEI+LTK
Sbjct: 123 SQEED-LGEVDYAKFFSIDSKDLRRVIRNLPIFHGDSICVTATGSQVKFSIASKEIVLTK 182

Query: 181 EVR-FIC 187
           E + FIC
Sbjct: 183 EKKLFIC 188

BLAST of Spg006200 vs. NCBI nr
Match: KAG7013723.1 (hypothetical protein SDJN02_23890, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 232.6 bits (592), Expect = 2.7e-57
Identity = 121/182 (66.48%), Postives = 149/182 (81.87%), Query Frame = 0

Query: 1   MFLVRLENFEPLVDATSILAQIAKDADAKFTPRMFLIIASHSSPRFVATLQMSRRFFTNY 60
           MFLV+L+ FEPL+DATS+L QI+KDAD +FT  M  +IASH+SPRFVATLQMS R FTNY
Sbjct: 1   MFLVQLQQFEPLLDATSLLTQISKDADVRFTAFMLSLIASHTSPRFVATLQMSCRLFTNY 60

Query: 61  LIDHNHVSRISLESFHDAMLDGGSYSSMTMHLLENINQMVLRFD-ASGHAPPLHHELTLS 120
            +DH + S++SLESFHDAMLDGGS+SSM++H+LE+  QM+LR++  S + PPLH EL LS
Sbjct: 61  SVDHYYSSKVSLESFHDAMLDGGSFSSMSIHVLESRQQMILRYENPSSNDPPLHLELPLS 120

Query: 121 PSQEDDILGQVTYGKYFTVHSKELRRIIKELPLFQADSVCVTVTSSRIKFSIASKEIILT 180
           P Q +  LGQV YGK+FTV+SK LR+IIKELPLF  D V V  TS+R+KFSIASKEI +T
Sbjct: 121 PPQAES-LGQVEYGKFFTVNSKVLRKIIKELPLFHDDLVRVAATSTRVKFSIASKEITVT 180

Query: 181 KE 182
           KE
Sbjct: 181 KE 181

BLAST of Spg006200 vs. NCBI nr
Match: XP_031744160.1 (uncharacterized protein LOC116404808 [Cucumis sativus])

HSP 1 Score: 230.7 bits (587), Expect = 1.0e-56
Identity = 114/181 (62.98%), Postives = 142/181 (78.45%), Query Frame = 0

Query: 1   MFLVRLENFEPLVDATSILAQIAKDADAKFTPRMFLIIASHSSPRFVATLQMSRRFFTNY 60
           MFLVRL+NFEP   ATS LA IA++AD KFTP  F I  S+  PRFVA L M+   F NY
Sbjct: 1   MFLVRLKNFEPFFHATSRLALIAREADVKFTPLFFSITVSNQFPRFVAYLVMTYNCFINY 60

Query: 61  LIDHNHVSRISLESFHDAMLDGGSYSSMTMHLLENINQMVLRFDASGHAPPLHHELTLSP 120
            +D++H SRISLESFHDA+LDGG   SMT+HLL NINQM+LRF++S HAP + HEL+L P
Sbjct: 61  KVDNDHTSRISLESFHDALLDGGGSPSMTIHLLANINQMILRFESSSHAPQVRHELSLKP 120

Query: 121 SQEDDILGQVTYGKYFTVHSKELRRIIKELPLFQADSVCVTVTSSRIKFSIASKEIILTK 180
           SQE+D LG++ Y K+F++ SK LRR+I+ LP+F  DS+CVT T S++KFSIASKEI+LTK
Sbjct: 121 SQEED-LGEIDYAKFFSIDSKALRRVIRNLPIFHGDSICVTATRSQVKFSIASKEIVLTK 180

Query: 181 E 182
           E
Sbjct: 181 E 180

BLAST of Spg006200 vs. NCBI nr
Match: XP_038875055.1 (uncharacterized protein LOC120067580 [Benincasa hispida])

HSP 1 Score: 229.9 bits (585), Expect = 1.7e-56
Identity = 121/182 (66.48%), Postives = 146/182 (80.22%), Query Frame = 0

Query: 1   MFLVRLENFEPLVDATSILAQIAKDADAKFTPRMFLIIASHSSPRFVATLQMSRRFFTNY 60
           MFLV+L NFEPL+DATS LAQI+  AD KFTP  F +IA + SPRFVATLQ+S++ FTNY
Sbjct: 1   MFLVKLTNFEPLLDATSYLAQISNYADVKFTPLEFYLIAPYPSPRFVATLQLSQKCFTNY 60

Query: 61  LIDHNHVSRISLESFHDAMLDGGSYSSMTMHLLENINQMVLRFDA-SGHAPPLHHELTLS 120
            +DH H S++ LESFHDA+LDGGS++SMT+HLLE  NQM+LRF   S   PPLHHELT S
Sbjct: 61  SVDHEHTSKVDLESFHDAILDGGSFASMTIHLLEKPNQMILRFQTPSSEIPPLHHELTFS 120

Query: 121 PSQ--EDDILGQVTYGKYFTVHSKELRRIIKELPLFQADS-VCVTVTSSRIKFSIASKEI 179
           P Q  +++I GQ+  GK+F V S+ LRRIIKELP+FQ DS VCV VTSS+IKFSIASKEI
Sbjct: 121 PPQLADNNIGGQLEEGKFFIVKSEALRRIIKELPIFQDDSVVCVGVTSSQIKFSIASKEI 180

BLAST of Spg006200 vs. ExPASy TrEMBL
Match: A0A1S3C8J1 (uncharacterized protein LOC103498010 OS=Cucumis melo OX=3656 GN=LOC103498010 PE=4 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 1.9e-64
Identity = 129/182 (70.88%), Postives = 152/182 (83.52%), Query Frame = 0

Query: 1   MFLVRLENFEPLVDATSILAQIAKDADAKFTPRMFLIIASHSSPRFVATLQMSRRFFTNY 60
           MFLVRLE FEPL+DATS+LAQ+AKDAD KFTP M +II S+ SP+FVATLQ+SRR FTN+
Sbjct: 1   MFLVRLEQFEPLIDATSLLAQVAKDADVKFTPLMLMIIVSNRSPQFVATLQLSRRLFTNF 60

Query: 61  LIDHNHVSRISLESFHDAMLDGGSYSSMTMHLLENINQMVLRFDASGH-APPLHHELTLS 120
            +DHN  S++SL+ FHDAMLDGGS+SSMT+HLL+  NQMVLRF+   H  PPLHHEL LS
Sbjct: 61  SVDHNKSSKVSLQPFHDAMLDGGSFSSMTIHLLDTTNQMVLRFETPSHDVPPLHHELALS 120

Query: 121 PSQEDDILGQVTYGKYFTVHSKELRRIIKELPLFQADSVCVTVTSSRIKFSIASKEIILT 180
           P Q ++ LGQV YG +FTV S+ELRRIIKELPLF  D+V VTVT S++KFSI SKEIILT
Sbjct: 121 PPQAEN-LGQVEYGNFFTVTSRELRRIIKELPLFHQDTVSVTVTGSQVKFSIQSKEIILT 180

Query: 181 KE 182
           KE
Sbjct: 181 KE 181

BLAST of Spg006200 vs. ExPASy TrEMBL
Match: A0A1S4E4N8 (uncharacterized protein LOC103502263 OS=Cucumis melo OX=3656 GN=LOC103502263 PE=4 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 3.9e-62
Identity = 122/187 (65.24%), Postives = 156/187 (83.42%), Query Frame = 0

Query: 1   MFLVRLENFEPLVDATSILAQIAKDADAKFTPRMFLIIASHSSPRFVATLQMSRRFFTNY 60
           MFLVRL++F+PL DATS LAQIA++AD KFTP  F IIAS+ SPRFVA LQM+   F NY
Sbjct: 3   MFLVRLKDFDPLFDATSRLAQIAREADIKFTPLFFSIIASNRSPRFVAYLQMTHHCFINY 62

Query: 61  LIDHNHVSRISLESFHDAMLDGGSYSSMTMHLLENINQMVLRFDASGHAPPLHHELTLSP 120
            +D++H SRISLESFHDA+LDGG+  SMT+HLL NI Q++LRF++S HAP +HHEL+L+P
Sbjct: 63  KVDNDHTSRISLESFHDALLDGGASPSMTIHLLANIKQLILRFESSSHAPKVHHELSLTP 122

Query: 121 SQEDDILGQVTYGKYFTVHSKELRRIIKELPLFQADSVCVTVTSSRIKFSIASKEIILTK 180
           SQE+D LG+V Y K+F++ SK+LRR+I+ LP+F  DS+CVT T S++KFSIASKEI+LTK
Sbjct: 123 SQEED-LGEVDYAKFFSIDSKDLRRVIRNLPIFHGDSICVTATGSQVKFSIASKEIVLTK 182

Query: 181 EVR-FIC 187
           E + FIC
Sbjct: 183 EKKLFIC 188

BLAST of Spg006200 vs. ExPASy TrEMBL
Match: A0A5D3DZ07 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1810G00070 PE=4 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 7.9e-55
Identity = 121/186 (65.05%), Postives = 151/186 (81.18%), Query Frame = 0

Query: 1   MFLVRLENFEPLVDATSILAQIAKD-ADAKFTPRMFLIIASHSSPRFVATLQMSRRFFTN 60
           MFLV+L+NF+PL+DATS LAQI+ D AD KFTP  F IIASH SPRF+ATLQ+S ++FT 
Sbjct: 1   MFLVKLKNFDPLLDATSFLAQISFDNADLKFTPSKFFIIASHRSPRFIATLQLSPQWFTT 60

Query: 61  YLIDHNHVSRISLESFHDAMLDGGSYSSMTMHLLENINQMVLRFDA-SGHAPPLHHELTL 120
           + +D++H S++SLESFHDA+LDGGS++SMT+HLL+  NQM+LRFD  S    PLHHELTL
Sbjct: 61  FSVDNDHSSKVSLESFHDAILDGGSFASMTIHLLDKTNQMILRFDTPSSEIQPLHHELTL 120

Query: 121 SPSQ-EDDILGQ--VTYGKYFTVHSKELRRIIKELPLFQADS-VCVTVTSSRIKFSIASK 180
           SP Q ED+ +GQ  +   KYF V SK LRRIIK+LP+FQ DS + V VT+SR+KFSIASK
Sbjct: 121 SPPQAEDNQIGQHELDERKYFIVKSKALRRIIKDLPIFQNDSIISVDVTNSRVKFSIASK 180

BLAST of Spg006200 vs. ExPASy TrEMBL
Match: A0A1S3CL88 (uncharacterized protein LOC103502250 OS=Cucumis melo OX=3656 GN=LOC103502250 PE=4 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 7.9e-55
Identity = 121/186 (65.05%), Postives = 151/186 (81.18%), Query Frame = 0

Query: 1   MFLVRLENFEPLVDATSILAQIAKD-ADAKFTPRMFLIIASHSSPRFVATLQMSRRFFTN 60
           MFLV+L+NF+PL+DATS LAQI+ D AD KFTP  F IIASH SPRF+ATLQ+S ++FT 
Sbjct: 1   MFLVKLKNFDPLLDATSFLAQISFDNADLKFTPSKFFIIASHRSPRFIATLQLSPQWFTT 60

Query: 61  YLIDHNHVSRISLESFHDAMLDGGSYSSMTMHLLENINQMVLRFDA-SGHAPPLHHELTL 120
           + +D++H S++SLESFHDA+LDGGS++SMT+HLL+  NQM+LRFD  S    PLHHELTL
Sbjct: 61  FSVDNDHSSKVSLESFHDAILDGGSFASMTIHLLDKTNQMILRFDTPSSEIQPLHHELTL 120

Query: 121 SPSQ-EDDILGQ--VTYGKYFTVHSKELRRIIKELPLFQADS-VCVTVTSSRIKFSIASK 180
           SP Q ED+ +GQ  +   KYF V SK LRRIIK+LP+FQ DS + V VT+SR+KFSIASK
Sbjct: 121 SPPQAEDNQIGQHELDERKYFIVKSKALRRIIKDLPIFQNDSIISVDVTNSRVKFSIASK 180

BLAST of Spg006200 vs. ExPASy TrEMBL
Match: A0A5D3BTK3 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00820 PE=4 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 7.4e-53
Identity = 115/182 (63.19%), Postives = 135/182 (74.18%), Query Frame = 0

Query: 1   MFLVRLENFEPLVDATSILAQIAKDADAKFTPRMFLIIASHSSPRFVATLQMSRRFFTNY 60
           MFLVRLE FEPL+DATS+LAQ+AKDAD KFTP M +II S+ SP+FVATLQ+SRR FTN+
Sbjct: 1   MFLVRLEQFEPLIDATSLLAQVAKDADVKFTPLMLMIIVSNRSPQFVATLQLSRRLFTNF 60

Query: 61  LIDHNHVSRISLESFHDAMLDGGSYSSMTMHLLENINQMVLRFDASGHAPPLHHELTLSP 120
            +DHN  S++SL+ FHDAMLDGGS+                        PPLHHEL LSP
Sbjct: 61  SVDHNKSSKVSLQPFHDAMLDGGSHD----------------------VPPLHHELALSP 120

Query: 121 SQEDDILGQVTYGKYFTVHSKELRRIIKELPLFQADSVCVTVTSSRIKFSIASKEIILTK 180
            Q ++ LGQV YG +FTV S+ELRRIIKELPLF  D+V VTVT S++KFSI SKEIILTK
Sbjct: 121 PQAEN-LGQVEYGNFFTVTSRELRRIIKELPLFHQDTVSVTVTGSQVKFSIQSKEIILTK 159

Query: 181 EV 183
           EV
Sbjct: 181 EV 159

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008458682.13.9e-6470.88PREDICTED: uncharacterized protein LOC103498010 [Cucumis melo][more]
XP_016903187.18.0e-6265.24PREDICTED: uncharacterized protein LOC103502263 [Cucumis melo][more]
KAG7013723.12.7e-5766.48hypothetical protein SDJN02_23890, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_031744160.11.0e-5662.98uncharacterized protein LOC116404808 [Cucumis sativus][more]
XP_038875055.11.7e-5666.48uncharacterized protein LOC120067580 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A1S3C8J11.9e-6470.88uncharacterized protein LOC103498010 OS=Cucumis melo OX=3656 GN=LOC103498010 PE=... [more]
A0A1S4E4N83.9e-6265.24uncharacterized protein LOC103502263 OS=Cucumis melo OX=3656 GN=LOC103502263 PE=... [more]
A0A5D3DZ077.9e-5565.05LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S3CL887.9e-5565.05uncharacterized protein LOC103502250 OS=Cucumis melo OX=3656 GN=LOC103502250 PE=... [more]
A0A5D3BTK37.4e-5363.19Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.70.10.10coord: 1..184
e-value: 2.3E-20
score: 75.0
NoneNo IPR availableSUPERFAMILY55979DNA clampcoord: 1..105

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg006200.1Spg006200.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane