Spg005884 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg005884
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRHOMBOID-like protein
Locationscaffold11: 1955858 .. 1959553 (-)
RNA-Seq ExpressionSpg005884
SyntenySpg005884
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAATTGGAGTGGAAGTTAAATGGAAATTTTGATTTTGGCCCAAAAATGTTGACAAAAAGAGGTTGATTTTGACGCCTTCGGCTGCCTATTCCCTCCAAATCAACCCAACATTGAACTCAAAGCACCAATCCCCTCGATCTCTTCTCCTTAATTTCAATTCCCATTGGTTCAATCGATTCTGCAATTCGTCCCAAATGGGATTCCTCCGCTTCTAGGATTTGATTAGACATCTTTTGTTTCTAATCCAATCTTGTCAATGGCGAGGGATAAACCCACCGCCGGCTTTGAAATCAAGGTCCATCCGCGTCATGGGGAACCGGAAGTTCACCCAGCGGAGCGTGACCCTTTGGCGGCGGCCGCCAGATCACGGCCGTCGGGGAGTTCTCCGGCCGGATTTGGCCAGTTCCGGCCGTTTAAGAAGTGGGTTCCATGGCTGGTGCCGGTCTTCGTGGTTGCCAACTCCGTTCTGTTTTCGATTTCTATGTACGTTAACGATTGCCCTAAGAACTCTGCCTCTTGCGTGGGGAGGTTTTTGGGCAGGTTCTCGTTTCAGCCCCTTAAAGAGAATCCCCTTCTTGGCCCTTCTTCTTCGACGTGAGTTTCTATTTTCTACCATTGGGTTTGAAATTAATCTACTGTTTTATTGAGGGAGGATGAGTGATGATAATGAGTGTGGATTCTCTCCAATATAGCATTTAATTGTGTTCATGCTCTCTGAGTCTGGAATTTCTTGTTGTGTTCAAATCATCTGAGGAGCATTGTGTTCTGTTCTATCTAGTTTCATAACTGCTGTAGAGATGGCAATGTTTTTGTGTTGTTTGAATGTAATTTGGAGATGATCATGGCTGCATTGAAGTATTTCTTTGTTGACAAAATGATGGGTATAACAAAAGTAGTTGGTGTGATGAAGTTTTGGGTAGGTGTAACAAGAGGCATTTATGGGATTTACCCAACGAAAGGTTTCCTTTTGAGGGTGCTAGGTGAAACTTTGATGACATTGCTGGTGAATTAGTTTAGAATTATGTGGGTGAATTCGTCTGCTGTATGAGTGATAAGTGCAAGAAGAAAAGGTGTATAGATTGTAGTAGAAGATATTGTAGTTATGTTTTCTTTCTCACTTTATTTCATTTCCAAATTTCAGATTAGAAAAGATGGGTGCTCTGGAAGTTGATAAAGTAGTTTATGGGCACCAGCCATGGAGATTGATCTCTTGTATATGGCTACATGCAGGGGTGTTCCATATACTTGCTAACATGCTCAGTCTTGTTTTCATTGGAATTCGGCTCGAGCAAGAATTTGGTTTTGGTATGCGTGCTTTTAGTTTGTAATTGTCTTCAAAATTCAAAGCCTTTCTGGAAATCATAGTTTCAGTTTACGAATGCCCCATTTGGTGACATTTTTGTTTTGGTTTTCTGTTTTTAGAAACCATGCTTACAAACTAACATTTTTCTTTGTTTTCTAATCTAGTTATTGGAATCTTAGCCAAAATTTGAAAACTAAAAGTAGTTTTTTCAAAAACTTATTTATTTCATAAGTGTATTACTAAACAGAGAAAGCATACGTATATGTAGTTTTTATAACCTTAGATTTCAGAAACTGTAAAACCAACAATGTAGTTGTCATAAGGCGCAAGCTTTTTGTGATGACCCTCAATCAGCTTCTATATAGTATTCTTTCGGTTGACTTCTTATGGATTTTGATTTTTTATCTAGCAAACATATTTGAGACAAGAATTAATGTAATCATTTAACTGCATATTGTGAATTGGTCATGAATTATTTTATTATCTATATAACAAGTAGAACCAATAAAAGGATAAAGAAAATAAAGCTAACCCTTTTTCTTTCCTGATAACAGCTTCATATTCTTACTGTAGAACAGTGATAACTCTTTTCTGCATTCTTGTTTTCTTATCTATAGTTCGAATTGGGATGCTGTACATTGTATCTGGGTTTGGCGGGAGTTTGTTATCGGCTCTGTTTATTCAGTCAAGTATCTCAGTAGGTGCTTCAGGTGCTCTTTTTGGTCTCCTTGGAGGGATGTTGTCAGAGCTCCTTACGAATTGGACAATATATGCAAATAAGGTAAAGGAAGTTCGAAATAAAAAAGTCACTCGACAGTCTTTTCATTGTCTAGTAACTGACTCAAAATCTGTGATGAAGCTTGCAGCTTTGTTGACTCTCCTCTTCATCATCGTAGTCAATTTGGCAGTGGGAGTACTTCCTCACGTTGATAATTTTGCTCATATTGGAGGATTTATCTCGGGTTTTCTTCTCGGATTCGTGTTTTTGGTACGTCCTCAGTTTGGGTGGGTTAGCCAACGAAATGCACCTCGTGGACGTAGTTCAACTTCAAAATCTAAATATAAGCCATATCAGTATGTGCTATGGATTGGATCTCTGTTGTTACTGATTGCTGGGTAAATGCTCATTTTTCATTTCATATAATTTGAAGTTCCAGTTGAATTGCTGAATTTGTATATTTGTTTTCTTCAAGTTGGTCCCTGCGTTCATATTGAATCCTCTAATATCTGCTAGCATTAGTCGCACTCCATCGACATTACTTAACTGACATTTTGGAATCAACAGGTTTGCACTTGGTTTGGTCTTTCTCTTTAGAGGGGAAAATTTGAATGATCACTGTTCATGGTGCCATTATTTGAGCTGCGTTCCTACGTCAAAATGGAGCTGCAATTCACAGAAATTCTCATGTGAGGTAACTGACTGTACTTCCTTAGCCTCAAATCAGTTCAAATCCTAGTTTACCATGAACTTTAGACACCTTCACCAAATTGTCTGTAGCCTAAAGGCTCATGCCCAACCTAAAGTCAACATCTAACCAGGCCGAGTCATCTGTACATTAGACTGAATATGAATCTCTTTTTTCGAAACGGATGGGTCGGAAATGAGTTAGGGACCAAACCTCCTACAATGAATAATCCTATCCCATTTTTCCATATAGTTTGAGGTGATCTTTTGCAGATGAAATATCAAAATCAATTTCTCGACAAGCCTGTTTAGGAGACTGTATTCAAAATAATTTTCTGCTTTTGTTCTCAAAACTGCTTTTAAAAACTATGACGGGTGGTGTGTAAAGGTTTATCATTAATGTTATTGTGTATTTAAAATCACTCCCTTCAAGTTCAATATTGAAAGAATGGCTTCTGTTGTTCACCTGGAAAAGAAAAGAAGCTTGGCATTTCTAAACTTTGTAACGTTCGTGCAGTCGAGTCAGTTGGGGAATCAGCTGAACATAACGTGCCTAAGCAACGGGAGAAGCGGCTCGTATTCGTTAGCCAACAGTAGCTCCTCTGAGGTACAACAGATATGCTCTCAGCTCTGCAGTTGACGTAGGTAAGATATAAAGTTTATACAGAGAAAAAATGTGAAGACTTGATAGAGCTCTTCTGTCATTGTTTTATGAGCAAAGTGTATATGAAACATTAGTCATGTCTTCATACAACAACTAGTCTGTGCCAAGCTCAGATTATGGAAATAACACAACTTTTCATTCTCAGCTCTCAAACCTCAGAGTTCCCTGTATCTTTGTTCTTTAACTCACTTTGCAAACAGAGTTCATGTAAAAACTTACATAGGAGAAAGAAAATATATGTTTAAGAGGAGTTTGTTCTCAGGAATGATTCTTTTCCCTTATGACTGCCCTTTTGACAAACTTCTTTTCCCTCCAAAATGTGTC

mRNA sequence

ATGGCGAGGGATAAACCCACCGCCGGCTTTGAAATCAAGGTCCATCCGCGTCATGGGGAACCGGAAGTTCACCCAGCGGAGCGTGACCCTTTGGCGGCGGCCGCCAGATCACGGCCGTCGGGGAGTTCTCCGGCCGGATTTGGCCAGTTCCGGCCGTTTAAGAAGTGGGTTCCATGGCTGGTGCCGGTCTTCGTGGTTGCCAACTCCGTTCTGTTTTCGATTTCTATGTACGTTAACGATTGCCCTAAGAACTCTGCCTCTTGCGTGGGGAGGTTTTTGGGCAGGTTCTCGTTTCAGCCCCTTAAAGAGAATCCCCTTCTTGGCCCTTCTTCTTCGACAGATGGCAATGTTTTTGTGTTGTTTGAATGTAATTTGGAGATGATCATGGCTGCATTGAAGTATTTCTTTGTTGACAAAATGATGGGTATAACAAAAGTAGTTGGTGTGATGAAGTTTTGGAATTATGTGGGTGAATTCGTCTGCTGTATGAGTGATAAGTGCAAGAAGAAAAGATTAGAAAAGATGGGTGCTCTGGAAGTTGATAAAGTAGTTTATGGGCACCAGCCATGGAGATTGATCTCTTGTATATGGCTACATGCAGGGGTGTTCCATATACTTGCTAACATGCTCAGTCTTGTTTTCATTGGAATTCGGCTCGAGCAAGAATTTGGTTTTGCTTCATATTCTTACTGTAGAACAGTGATAACTCTTTTCTGCATTCTTGTTTTCTTATCTATAGTTCGAATTGGGATGCTGTACATTGTATCTGGGTTTGGCGGGAGTTTGTTATCGGCTCTGTTTATTCAGTCAAGTATCTCAGTAGGTGCTTCAGGTGCTCTTTTTGGTCTCCTTGGAGGGATGTTGTCAGAGCTCCTTACGAATTGGACAATATATGCAAATAAGCTTGCAGCTTTGTTGACTCTCCTCTTCATCATCGTAGTCAATTTGGCAGTGGGAGTACTTCCTCACGTTGATAATTTTGCTCATATTGGAGGATTTATCTCGGGTTTTCTTCTCGGATTCGTGTTTTTGGTACGTCCTCAGTTTGGGTGGGTTAGCCAACGAAATGCACCTCGTGGACGTAGTTCAACTTCAAAATCTAAATATAAGCCATATCAGTATGTGCTATGGATTGGATCTCTGTTGTTACTGATTGCTGGGTTTGCACTTGGTTTGGTCTTTCTCTTTAGAGGGGAAAATTTGAATGATCACTGTTCATGGTGCCATTATTTGAGCTGCGTTCCTACGTCAAAATGGAGCTGCAATTCACAGAAATTCTCATGTGAGTCGAGTCAGTTGGGGAATCAGCTGAACATAACGTGCCTAAGCAACGGGAGAAGCGGCTCGTATTCGTTAGCCAACAGTAGCTCCTCTGAGGTACAACAGATATGCTCTCAGCTCTGCAGTTGA

Coding sequence (CDS)

ATGGCGAGGGATAAACCCACCGCCGGCTTTGAAATCAAGGTCCATCCGCGTCATGGGGAACCGGAAGTTCACCCAGCGGAGCGTGACCCTTTGGCGGCGGCCGCCAGATCACGGCCGTCGGGGAGTTCTCCGGCCGGATTTGGCCAGTTCCGGCCGTTTAAGAAGTGGGTTCCATGGCTGGTGCCGGTCTTCGTGGTTGCCAACTCCGTTCTGTTTTCGATTTCTATGTACGTTAACGATTGCCCTAAGAACTCTGCCTCTTGCGTGGGGAGGTTTTTGGGCAGGTTCTCGTTTCAGCCCCTTAAAGAGAATCCCCTTCTTGGCCCTTCTTCTTCGACAGATGGCAATGTTTTTGTGTTGTTTGAATGTAATTTGGAGATGATCATGGCTGCATTGAAGTATTTCTTTGTTGACAAAATGATGGGTATAACAAAAGTAGTTGGTGTGATGAAGTTTTGGAATTATGTGGGTGAATTCGTCTGCTGTATGAGTGATAAGTGCAAGAAGAAAAGATTAGAAAAGATGGGTGCTCTGGAAGTTGATAAAGTAGTTTATGGGCACCAGCCATGGAGATTGATCTCTTGTATATGGCTACATGCAGGGGTGTTCCATATACTTGCTAACATGCTCAGTCTTGTTTTCATTGGAATTCGGCTCGAGCAAGAATTTGGTTTTGCTTCATATTCTTACTGTAGAACAGTGATAACTCTTTTCTGCATTCTTGTTTTCTTATCTATAGTTCGAATTGGGATGCTGTACATTGTATCTGGGTTTGGCGGGAGTTTGTTATCGGCTCTGTTTATTCAGTCAAGTATCTCAGTAGGTGCTTCAGGTGCTCTTTTTGGTCTCCTTGGAGGGATGTTGTCAGAGCTCCTTACGAATTGGACAATATATGCAAATAAGCTTGCAGCTTTGTTGACTCTCCTCTTCATCATCGTAGTCAATTTGGCAGTGGGAGTACTTCCTCACGTTGATAATTTTGCTCATATTGGAGGATTTATCTCGGGTTTTCTTCTCGGATTCGTGTTTTTGGTACGTCCTCAGTTTGGGTGGGTTAGCCAACGAAATGCACCTCGTGGACGTAGTTCAACTTCAAAATCTAAATATAAGCCATATCAGTATGTGCTATGGATTGGATCTCTGTTGTTACTGATTGCTGGGTTTGCACTTGGTTTGGTCTTTCTCTTTAGAGGGGAAAATTTGAATGATCACTGTTCATGGTGCCATTATTTGAGCTGCGTTCCTACGTCAAAATGGAGCTGCAATTCACAGAAATTCTCATGTGAGTCGAGTCAGTTGGGGAATCAGCTGAACATAACGTGCCTAAGCAACGGGAGAAGCGGCTCGTATTCGTTAGCCAACAGTAGCTCCTCTGAGGTACAACAGATATGCTCTCAGCTCTGCAGTTGA

Protein sequence

MARDKPTAGFEIKVHPRHGEPEVHPAERDPLAAAARSRPSGSSPAGFGQFRPFKKWVPWLVPVFVVANSVLFSISMYVNDCPKNSASCVGRFLGRFSFQPLKENPLLGPSSSTDGNVFVLFECNLEMIMAALKYFFVDKMMGITKVVGVMKFWNYVGEFVCCMSDKCKKKRLEKMGALEVDKVVYGHQPWRLISCIWLHAGVFHILANMLSLVFIGIRLEQEFGFASYSYCRTVITLFCILVFLSIVRIGMLYIVSGFGGSLLSALFIQSSISVGASGALFGLLGGMLSELLTNWTIYANKLAALLTLLFIIVVNLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFGWVSQRNAPRGRSSTSKSKYKPYQYVLWIGSLLLLIAGFALGLVFLFRGENLNDHCSWCHYLSCVPTSKWSCNSQKFSCESSQLGNQLNITCLSNGRSGSYSLANSSSSEVQQICSQLCS
Homology
BLAST of Spg005884 vs. NCBI nr
Match: XP_038895553.1 (RHOMBOID-like protein 1 [Benincasa hispida])

HSP 1 Score: 669.8 bits (1727), Expect = 1.7e-188
Identity = 353/469 (75.27%), Postives = 367/469 (78.25%), Query Frame = 0

Query: 1   MARDKPTAGFEIKVHPRHGEPEVHPAERDPLAAAARSRPSGSSPAGFGQFRPFKKWVPWL 60
           MARDKPTAG EIKVH RHGEPEV P        AARSRPSG+SP  FGQ+RPFKKWVPWL
Sbjct: 1   MARDKPTAGIEIKVHSRHGEPEVQP-------VAARSRPSGTSPTEFGQYRPFKKWVPWL 60

Query: 61  VPVFVVANSVLFSISMYVNDCPKNSASCVGRFLGRFSFQPLKENPLLGPSSSTDGNVFVL 120
           VPVFVVANSVLF+ISMYVNDCPKNSASCVGRFLGRFSFQPLKENPLLGPSSST       
Sbjct: 61  VPVFVVANSVLFTISMYVNDCPKNSASCVGRFLGRFSFQPLKENPLLGPSSST------- 120

Query: 121 FECNLEMIMAALKYFFVDKMMGITKVVGVMKFWNYVGEFVCCMSDKCKKKRLEKMGALEV 180
                                                              LEKMGALEV
Sbjct: 121 ---------------------------------------------------LEKMGALEV 180

Query: 181 DKVVYGHQPWRLISCIWLHAGVFHILANMLSLVFIGIRLEQEFGFASYSYCRTVITLFCI 240
           DKVVYGHQPWRLISC+WLHAGVFHILANMLSLVFIGIRLEQEFGF               
Sbjct: 181 DKVVYGHQPWRLISCLWLHAGVFHILANMLSLVFIGIRLEQEFGF--------------- 240

Query: 241 LVFLSIVRIGMLYIVSGFGGSLLSALFIQSSISVGASGALFGLLGGMLSELLTNWTIYAN 300
                 VRIGMLYIVSGFGGSL+SALFIQS ISVGASGALFGLLGGMLSELLTNWTIYAN
Sbjct: 241 ------VRIGMLYIVSGFGGSLMSALFIQSGISVGASGALFGLLGGMLSELLTNWTIYAN 300

Query: 301 KLAALLTLLFIIVVNLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFGWVSQRNAPRG 360
           KLAALLTLLFIIV+NLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFGWVSQRNAPRG
Sbjct: 301 KLAALLTLLFIIVINLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFGWVSQRNAPRG 360

Query: 361 RSSTSKSKYKPYQYVLWIGSLLLLIAGFALGLVFLFRGENLNDHCSWCHYLSCVPTSKWS 420
            +STSKSKYKPYQYVLW+ SL+LLIAGFA+GL FLFRGEN+ND CSWCHYLSC+PTSKWS
Sbjct: 361 NNSTSKSKYKPYQYVLWVVSLVLLIAGFAVGLAFLFRGENMNDRCSWCHYLSCIPTSKWS 383

Query: 421 CNSQKFSCESSQLGNQLNITCLSNGRSGSYSLANSSSSEVQQICSQLCS 470
           CNS K SCE+SQLGNQLN+TCLSNGRSGSYSL NSSS E QQICSQLCS
Sbjct: 421 CNSPKLSCETSQLGNQLNMTCLSNGRSGSYSLTNSSSFEAQQICSQLCS 383

BLAST of Spg005884 vs. NCBI nr
Match: XP_008457471.1 (PREDICTED: RHOMBOID-like protein 1 [Cucumis melo] >XP_008457472.1 PREDICTED: RHOMBOID-like protein 1 [Cucumis melo] >KAA0067746.1 RHOMBOID-like protein 1 [Cucumis melo var. makuwa] >TYJ97398.1 RHOMBOID-like protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 669.5 bits (1726), Expect = 2.2e-188
Identity = 352/469 (75.05%), Postives = 369/469 (78.68%), Query Frame = 0

Query: 1   MARDKPTAGFEIKVHPRHGEPEVHPAERDPLAAAARSRPSGSSPAGFGQFRPFKKWVPWL 60
           MAR+KPTAG EIKVH RHGE EV P       AAARSRPSG+SP  FGQ+RPFKKWVPWL
Sbjct: 1   MARNKPTAGIEIKVHSRHGEHEVQP-------AAARSRPSGTSPTEFGQYRPFKKWVPWL 60

Query: 61  VPVFVVANSVLFSISMYVNDCPKNSASCVGRFLGRFSFQPLKENPLLGPSSSTDGNVFVL 120
           VPVFVVANSV+F+ISMYVNDCPKNSASCVGRFLGRFSFQPLKENPLLGPSSST       
Sbjct: 61  VPVFVVANSVMFTISMYVNDCPKNSASCVGRFLGRFSFQPLKENPLLGPSSST------- 120

Query: 121 FECNLEMIMAALKYFFVDKMMGITKVVGVMKFWNYVGEFVCCMSDKCKKKRLEKMGALEV 180
                                                              LEKMGALEV
Sbjct: 121 ---------------------------------------------------LEKMGALEV 180

Query: 181 DKVVYGHQPWRLISCIWLHAGVFHILANMLSLVFIGIRLEQEFGFASYSYCRTVITLFCI 240
           DKVVYGHQPWRLISC+WLHAGVFHILANMLSL+FIGIRLEQEFGF               
Sbjct: 181 DKVVYGHQPWRLISCLWLHAGVFHILANMLSLIFIGIRLEQEFGF--------------- 240

Query: 241 LVFLSIVRIGMLYIVSGFGGSLLSALFIQSSISVGASGALFGLLGGMLSELLTNWTIYAN 300
                 VRIGMLYIVSGFGGSL+SALFIQS ISVGASGALFGLLGGMLSELLTNWTIYAN
Sbjct: 241 ------VRIGMLYIVSGFGGSLMSALFIQSGISVGASGALFGLLGGMLSELLTNWTIYAN 300

Query: 301 KLAALLTLLFIIVVNLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFGWVSQRNAPRG 360
           KLAALLTLLFIIV+NLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFGWVSQRNAPRG
Sbjct: 301 KLAALLTLLFIIVINLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFGWVSQRNAPRG 360

Query: 361 RSSTSKSKYKPYQYVLWIGSLLLLIAGFALGLVFLFRGENLNDHCSWCHYLSCVPTSKWS 420
             STSKSKYKPYQYVLW+ SL+LLIAGFA+GLVFLFRGEN+ND CSWCHYLSC+PTSKWS
Sbjct: 361 NRSTSKSKYKPYQYVLWVVSLVLLIAGFAVGLVFLFRGENMNDRCSWCHYLSCIPTSKWS 383

Query: 421 CNSQKFSCESSQLGNQLNITCLSNGRSGSYSLANSSSSEVQQICSQLCS 470
           CNSQKFSCE+SQLGNQLN+TCLSNGR GSYSL NSSSSE +QICSQLCS
Sbjct: 421 CNSQKFSCETSQLGNQLNMTCLSNGRRGSYSLTNSSSSEAEQICSQLCS 383

BLAST of Spg005884 vs. NCBI nr
Match: XP_004153913.1 (RHOMBOID-like protein 1 isoform X1 [Cucumis sativus] >KGN65729.1 hypothetical protein Csa_020033 [Cucumis sativus])

HSP 1 Score: 669.1 bits (1725), Expect = 2.8e-188
Identity = 352/469 (75.05%), Postives = 370/469 (78.89%), Query Frame = 0

Query: 1   MARDKPTAGFEIKVHPRHGEPEVHPAERDPLAAAARSRPSGSSPAGFGQFRPFKKWVPWL 60
           MARDKPTAG EIKVH RHGE EV P       AAARSRPSG+SP  FGQ+RPFKKWVPWL
Sbjct: 1   MARDKPTAGIEIKVHSRHGEHEVQP-------AAARSRPSGTSPIEFGQYRPFKKWVPWL 60

Query: 61  VPVFVVANSVLFSISMYVNDCPKNSASCVGRFLGRFSFQPLKENPLLGPSSSTDGNVFVL 120
           VPVFVVANSV+F+ISMYVNDCPKNSASC+GRFLGRFSFQPLKENPLLGPSSST       
Sbjct: 61  VPVFVVANSVMFTISMYVNDCPKNSASCIGRFLGRFSFQPLKENPLLGPSSST------- 120

Query: 121 FECNLEMIMAALKYFFVDKMMGITKVVGVMKFWNYVGEFVCCMSDKCKKKRLEKMGALEV 180
                                                              LEKMGALEV
Sbjct: 121 ---------------------------------------------------LEKMGALEV 180

Query: 181 DKVVYGHQPWRLISCIWLHAGVFHILANMLSLVFIGIRLEQEFGFASYSYCRTVITLFCI 240
           DKVVYGHQ WRLISC+WLHAGVFHILANMLSLVFIGIRLEQEFGF               
Sbjct: 181 DKVVYGHQAWRLISCLWLHAGVFHILANMLSLVFIGIRLEQEFGF--------------- 240

Query: 241 LVFLSIVRIGMLYIVSGFGGSLLSALFIQSSISVGASGALFGLLGGMLSELLTNWTIYAN 300
                 VRIGMLYIVSGFGGSL+SALFIQS ISVGASGALFGLLGGMLSELLTNWTIYAN
Sbjct: 241 ------VRIGMLYIVSGFGGSLMSALFIQSGISVGASGALFGLLGGMLSELLTNWTIYAN 300

Query: 301 KLAALLTLLFIIVVNLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFGWVSQRNAPRG 360
           KLAALLTLLFIIV+NLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFGWVSQRNAPRG
Sbjct: 301 KLAALLTLLFIIVINLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFGWVSQRNAPRG 360

Query: 361 RSSTSKSKYKPYQYVLWIGSLLLLIAGFALGLVFLFRGENLNDHCSWCHYLSCVPTSKWS 420
            SSTSKSKYKPYQYVLW+ SL+LLIAGFA+GLVFLFRGEN+ND CSWCHYLSC+PTSKWS
Sbjct: 361 NSSTSKSKYKPYQYVLWVVSLVLLIAGFAVGLVFLFRGENMNDRCSWCHYLSCIPTSKWS 383

Query: 421 CNSQKFSCESSQLGNQLNITCLSNGRSGSYSLANSSSSEVQQICSQLCS 470
           CNSQKFSCE+SQLGNQLN+TCLSNGRSGSYSL NSSS+E ++ICSQLCS
Sbjct: 421 CNSQKFSCETSQLGNQLNMTCLSNGRSGSYSLTNSSSTEAEKICSQLCS 383

BLAST of Spg005884 vs. NCBI nr
Match: XP_022964679.1 (RHOMBOID-like protein 1 [Cucurbita moschata])

HSP 1 Score: 653.3 bits (1684), Expect = 1.6e-183
Identity = 345/469 (73.56%), Postives = 362/469 (77.19%), Query Frame = 0

Query: 1   MARDKPTAGFEIKVHPRHGEPEVHPAERDPLAAAARSRPSGSSPAGFGQFRPFKKWVPWL 60
           MARDKP A  EIKVH RHG+P V P+     AAAARSRPS +   GFGQFRPFKKW+PWL
Sbjct: 1   MARDKPAANIEIKVHSRHGDPAVEPS-----AAAARSRPSRTPSTGFGQFRPFKKWIPWL 60

Query: 61  VPVFVVANSVLFSISMYVNDCPKNSASCVGRFLGRFSFQPLKENPLLGPSSSTDGNVFVL 120
           VPVFVV NSVLFSI+MYVNDCPKNSASC+G FLGRFSFQPLKENPLLGPS ST       
Sbjct: 61  VPVFVVVNSVLFSITMYVNDCPKNSASCIGTFLGRFSFQPLKENPLLGPSPST------- 120

Query: 121 FECNLEMIMAALKYFFVDKMMGITKVVGVMKFWNYVGEFVCCMSDKCKKKRLEKMGALEV 180
                                                              LEKMGALEV
Sbjct: 121 ---------------------------------------------------LEKMGALEV 180

Query: 181 DKVVYGHQPWRLISCIWLHAGVFHILANMLSLVFIGIRLEQEFGFASYSYCRTVITLFCI 240
           DKVVYGHQPWRLISC+WLHAGVFHILANMLSLV IGIRLEQEFGF               
Sbjct: 181 DKVVYGHQPWRLISCLWLHAGVFHILANMLSLVCIGIRLEQEFGF--------------- 240

Query: 241 LVFLSIVRIGMLYIVSGFGGSLLSALFIQSSISVGASGALFGLLGGMLSELLTNWTIYAN 300
                 VRIGMLYIVSGFGGSLLSALFIQS ISVGASGALFGLLGGMLSELLTNWTIY N
Sbjct: 241 ------VRIGMLYIVSGFGGSLLSALFIQSGISVGASGALFGLLGGMLSELLTNWTIYEN 300

Query: 301 KLAALLTLLFIIVVNLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFGWVSQRNAPRG 360
           KLAALLTL+FIIV+NLAVGVLPHVDNFAHIGGFISGF LGFVFLVRPQFGWVSQ+NAPR 
Sbjct: 301 KLAALLTLVFIIVINLAVGVLPHVDNFAHIGGFISGFFLGFVFLVRPQFGWVSQQNAPRR 360

Query: 361 RSSTSKSKYKPYQYVLWIGSLLLLIAGFALGLVFLFRGENLNDHCSWCHYLSCVPTSKWS 420
            SSTSK+KY PYQYVLWI SLLLLIAGFA+GLVFLFRGENLND CSWCHYLSC+PTSKWS
Sbjct: 361 HSSTSKAKYMPYQYVLWIASLLLLIAGFAVGLVFLFRGENLNDRCSWCHYLSCIPTSKWS 385

Query: 421 CNSQKFSCESSQLGNQLNITCLSNGRSGSYSLANSSSSEVQQICSQLCS 470
           CNSQKFSCE+SQLGNQLN+TCLSNGRSGSYSLANS+SSE QQIC+QLCS
Sbjct: 421 CNSQKFSCETSQLGNQLNMTCLSNGRSGSYSLANSNSSEAQQICTQLCS 385

BLAST of Spg005884 vs. NCBI nr
Match: XP_023519656.1 (RHOMBOID-like protein 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 652.1 bits (1681), Expect = 3.6e-183
Identity = 344/469 (73.35%), Postives = 362/469 (77.19%), Query Frame = 0

Query: 1   MARDKPTAGFEIKVHPRHGEPEVHPAERDPLAAAARSRPSGSSPAGFGQFRPFKKWVPWL 60
           MARDKP A  EIKVH RHG+P V P+     AAAARSRPS +   GFGQFRPFKKW+PWL
Sbjct: 1   MARDKPAANIEIKVHSRHGDPAVEPS-----AAAARSRPSRTPSTGFGQFRPFKKWIPWL 60

Query: 61  VPVFVVANSVLFSISMYVNDCPKNSASCVGRFLGRFSFQPLKENPLLGPSSSTDGNVFVL 120
           VPVFVV NSVLFSI+MYVNDCPKNSASC+G FLGRFSFQPLKENPLLGPS ST       
Sbjct: 61  VPVFVVVNSVLFSITMYVNDCPKNSASCIGTFLGRFSFQPLKENPLLGPSPST------- 120

Query: 121 FECNLEMIMAALKYFFVDKMMGITKVVGVMKFWNYVGEFVCCMSDKCKKKRLEKMGALEV 180
                                                              LEKMGALEV
Sbjct: 121 ---------------------------------------------------LEKMGALEV 180

Query: 181 DKVVYGHQPWRLISCIWLHAGVFHILANMLSLVFIGIRLEQEFGFASYSYCRTVITLFCI 240
           DKVVYGHQPWRLISC+WLHAGVFHILANMLSLV IGIRLEQEFGF               
Sbjct: 181 DKVVYGHQPWRLISCLWLHAGVFHILANMLSLVCIGIRLEQEFGF--------------- 240

Query: 241 LVFLSIVRIGMLYIVSGFGGSLLSALFIQSSISVGASGALFGLLGGMLSELLTNWTIYAN 300
                 VRIGMLYIVSGFGGSLLSALFIQS ISVGASGALFGLLGGMLSELLTNWTIY N
Sbjct: 241 ------VRIGMLYIVSGFGGSLLSALFIQSGISVGASGALFGLLGGMLSELLTNWTIYEN 300

Query: 301 KLAALLTLLFIIVVNLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFGWVSQRNAPRG 360
           KLAALLTL+FIIV+NLAVGVLPHVDNFAHIGGFISGF LGFVFLVRPQFGWVSQ+NAPR 
Sbjct: 301 KLAALLTLVFIIVINLAVGVLPHVDNFAHIGGFISGFFLGFVFLVRPQFGWVSQQNAPRR 360

Query: 361 RSSTSKSKYKPYQYVLWIGSLLLLIAGFALGLVFLFRGENLNDHCSWCHYLSCVPTSKWS 420
            SSTSK+KY PYQYVLWI SLLLLIAGFA+GLVFL+RGENLND CSWCHYLSC+PTSKWS
Sbjct: 361 HSSTSKAKYMPYQYVLWIASLLLLIAGFAVGLVFLYRGENLNDRCSWCHYLSCIPTSKWS 385

Query: 421 CNSQKFSCESSQLGNQLNITCLSNGRSGSYSLANSSSSEVQQICSQLCS 470
           CNSQKFSCE+SQLGNQLN+TCLSNGRSGSYSLANS+SSE QQIC+QLCS
Sbjct: 421 CNSQKFSCETSQLGNQLNMTCLSNGRSGSYSLANSNSSEAQQICTQLCS 385

BLAST of Spg005884 vs. ExPASy Swiss-Prot
Match: Q0WQX7 (RHOMBOID-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RBL1 PE=2 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 1.3e-140
Identity = 269/470 (57.23%), Postives = 316/470 (67.23%), Query Frame = 0

Query: 1   MARDKPTAGFEIK-VHPRHGEPEVHPAERDPLAAAARSRPSGSSPAGFGQFRPFKKWVPW 60
           MARD+   G EIK V+P          E  P  A  R R      A F +FRPFK W PW
Sbjct: 1   MARDR-REGLEIKVVNPPAAATNNVAVETSPATATRRRRQ--QQRASFAEFRPFKLWFPW 60

Query: 61  LVPVFVVANSVLFSISMYVNDCPKNSASCVGRFLGRFSFQPLKENPLLGPSSSTDGNVFV 120
           LVP  VVAN  LF+ISM++N+CPKNSA C+ RFLGRF+FQP+KENPLLGPSS T      
Sbjct: 61  LVPAIVVANIALFAISMFINNCPKNSAYCLARFLGRFAFQPMKENPLLGPSSLT------ 120

Query: 121 LFECNLEMIMAALKYFFVDKMMGITKVVGVMKFWNYVGEFVCCMSDKCKKKRLEKMGALE 180
                                                               LEKMGAL+
Sbjct: 121 ----------------------------------------------------LEKMGALD 180

Query: 181 VDKVVYGHQPWRLISCIWLHAGVFHILANMLSLVFIGIRLEQEFGFASYSYCRTVITLFC 240
           V  VV+ H+ WRL +CIWLHAGVFH+LANMLSL+FIGIRLEQEFGF              
Sbjct: 181 VSMVVHKHEVWRLFTCIWLHAGVFHVLANMLSLIFIGIRLEQEFGF-------------- 240

Query: 241 ILVFLSIVRIGMLYIVSGFGGSLLSALFIQSSISVGASGALFGLLGGMLSELLTNWTIYA 300
                  VRIG+LY++SGFGGSLLS+LF ++ ISVGASGALFGLLG MLSELLTNWTIYA
Sbjct: 241 -------VRIGLLYMISGFGGSLLSSLFNRAGISVGASGALFGLLGAMLSELLTNWTIYA 300

Query: 301 NKLAALLTLLFIIVVNLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFGWVSQRNAPR 360
           NK AALLTL+FII +NLAVG+LPHVDNFAH+GGF SGFLLGFVFL+RPQ+G+ +QRN PR
Sbjct: 301 NKFAALLTLIFIIAINLAVGILPHVDNFAHLGGFTSGFLLGFVFLIRPQYGYFNQRNNPR 360

Query: 361 GRSSTS-KSKYKPYQYVLWIGSLLLLIAGFALGLVFLFRGENLNDHCSWCHYLSCVPTSK 420
           G ++ S KSK+KPYQYVLWI SL+LLIAG+  GLV L RG +LN HCSWCHYLSC+PTS 
Sbjct: 361 GYAAPSAKSKHKPYQYVLWITSLVLLIAGYTAGLVVLLRGTDLNKHCSWCHYLSCIPTSL 388

Query: 421 WSCNSQKFSCESSQLGNQLNITCLSNGRSGSYSLANSSSSEVQQICSQLC 469
           WSC SQ   CESSQ+G Q+N+TC++NG++  Y L+N   S +QQ+CSQLC
Sbjct: 421 WSCKSQNVYCESSQIGQQMNLTCITNGKTEMYKLSNDIPSRIQQLCSQLC 388

BLAST of Spg005884 vs. ExPASy Swiss-Prot
Match: F4JBM4 (RHOMBOID-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=RBL4 PE=2 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 2.5e-115
Identity = 214/418 (51.20%), Postives = 269/418 (64.35%), Query Frame = 0

Query: 51  RPFKKWVPWLVPVFVVANSVLFSISMYVNDCPKNSASCVGRFLGRFSFQPLKENPLLGPS 110
           + F+ W PWL+P FVVAN  +F I+MYVN+CPK S  C   FLGRFSFQ  +ENPLLGPS
Sbjct: 59  KEFRSWFPWLIPCFVVANVAVFVITMYVNNCPKKSGDCFADFLGRFSFQNTRENPLLGPS 118

Query: 111 SSTDGNVFVLFECNLEMIMAALKYFFVDKMMGITKVVGVMKFWNYVGEFVCCMSDKCKKK 170
           S T                                                         
Sbjct: 119 SLT--------------------------------------------------------- 178

Query: 171 RLEKMGALEVDKVVYGHQPWRLISCIWLHAGVFHILANMLSLVFIGIRLEQEFGFASYSY 230
            L+ MG L+V KVV G + WRL+SC WLH GV H+L NML+L+FIGIR+E+EFGF     
Sbjct: 179 -LQTMGGLDVKKVVKGDEGWRLLSCNWLHGGVVHLLMNMLTLLFIGIRMEREFGF----- 238

Query: 231 CRTVITLFCILVFLSIVRIGMLYIVSGFGGSLLSALFIQSSISVGASGALFGLLGGMLSE 290
                           +RIG+LY++SGFGGS+LSALF++S+ISVGASGA+FGLLGGMLSE
Sbjct: 239 ----------------IRIGLLYLISGFGGSILSALFLRSNISVGASGAVFGLLGGMLSE 298

Query: 291 LLTNWTIYANKLAALLTLLFIIVVNLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFG 350
           +  NWTIY+NK+  ++TL+ I+ VNL +GVLP VDNFAHIGGF +GFLLGFV L+RP +G
Sbjct: 299 IFINWTIYSNKVVTIVTLVLIVAVNLGLGVLPGVDNFAHIGGFATGFLLGFVLLIRPHYG 358

Query: 351 WVSQRNAPRGRSSTSKSKYKPYQYVLWIGSLLLLIAGFALGLVFLFRGENLNDHCSWCHY 410
           W++QRN P         ++K YQ +LW  SLL+L+AGF +GL+ LF   + N+HCSWCHY
Sbjct: 359 WINQRNGP----GAKPHRFKIYQGILWTISLLILVAGFIVGLISLFNNVDGNEHCSWCHY 393

Query: 411 LSCVPTSKWSCNSQKFSCESSQLGNQLNITCLSNGRSGSYSLANSSSSEVQQICSQLC 469
           LSCVPTSKWSCN +  SC ++QLGNQL++TCL NG+S SY LAN S S +  +C QLC
Sbjct: 419 LSCVPTSKWSCNREPASCTTTQLGNQLSMTCLRNGKSASYILANPSDSRINSLCVQLC 393

BLAST of Spg005884 vs. ExPASy Swiss-Prot
Match: Q9CAN1 (RHOMBOID-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RBL2 PE=1 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 1.0e-103
Identity = 200/370 (54.05%), Postives = 237/370 (64.05%), Query Frame = 0

Query: 56  WVPWLVPVFVVANSVLFSISMYVNDCPKN----SASCVGRFLGRFSFQPLKENPLLGPSS 115
           W  WL+P  VVAN  +F   M+VNDCPK     +  CV RFLGRFSFQPLKENPL GPSS
Sbjct: 31  WTSWLIPAIVVANLAVFIAVMFVNDCPKKITGPNKECVARFLGRFSFQPLKENPLFGPSS 90

Query: 116 STDGNVFVLFECNLEMIMAALKYFFVDKMMGITKVVGVMKFWNYVGEFVCCMSDKCKKKR 175
           ST                                                          
Sbjct: 91  ST---------------------------------------------------------- 150

Query: 176 LEKMGALEVDKVVYGHQPWRLISCIWLHAGVFHILANMLSLVFIGIRLEQEFGFASYSYC 235
           LEKMGALE  KVV+ HQ WRL+SC+WLHAG+ H+L NMLSL+FIGIRLEQ+FGF      
Sbjct: 151 LEKMGALEWRKVVHEHQGWRLLSCMWLHAGIIHLLTNMLSLIFIGIRLEQQFGF------ 210

Query: 236 RTVITLFCILVFLSIVRIGMLYIVSGFGGSLLSALFIQSSISVGASGALFGLLGGMLSEL 295
                          +R+G++Y++SG GGS+LS+LF+Q SISVGASGALFGLLG MLSEL
Sbjct: 211 ---------------IRVGLIYLISGLGGSILSSLFLQESISVGASGALFGLLGAMLSEL 270

Query: 296 LTNWTIYANKLAALLTLLFIIVVNLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFGW 355
           LTNWTIYANK AAL+TLLFII +NLA+G+LP VDNFAHIGGF++GF LGFV LVRPQ+GW
Sbjct: 271 LTNWTIYANKAAALITLLFIIAINLALGMLPRVDNFAHIGGFLTGFCLGFVLLVRPQYGW 317

Query: 356 VSQRNAPRGRSSTSKSKYKPYQYVLWIGSLLLLIAGFALGLVFLFRGENLNDHCSWCHYL 415
                A R  +S +K KY  YQYVL++ S++LL+ G  + LV LF+GEN N HC WCHYL
Sbjct: 331 ----EASRTNTSRTKRKYSMYQYVLFVVSVVLLVVGLTVALVMLFKGENGNKHCKWCHYL 317

Query: 416 SCVPTSKWSC 422
           SC PTSKW+C
Sbjct: 391 SCFPTSKWTC 317

BLAST of Spg005884 vs. ExPASy Swiss-Prot
Match: Q9LYP1 (RHOMBOID-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RBL3 PE=2 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 1.3e-103
Identity = 200/387 (51.68%), Postives = 249/387 (64.34%), Query Frame = 0

Query: 42  SSPAGFGQFRPFKKWVPWLVPVFVVANSVLFSISMYVNDCPKNSAS------CVGRFLGR 101
           SS   FG      +W  WLVP+FVVAN  +F ++M+VN+CP +  S      CV +FLGR
Sbjct: 39  SSSTEFGDNALSSRWTSWLVPMFVVANVAVFVVAMFVNNCPNHFESHRLRGHCVAKFLGR 98

Query: 102 FSFQPLKENPLLGPSSSTDGNVFVLFECNLEMIMAALKYFFVDKMMGITKVVGVMKFWNY 161
            SF+PL+ NPL GPSS T                                          
Sbjct: 99  LSFEPLRTNPLFGPSSHT------------------------------------------ 158

Query: 162 VGEFVCCMSDKCKKKRLEKMGALEVDKVVYGHQPWRLISCIWLHAGVFHILANMLSLVFI 221
                           LEK+GALE  KVV   + WRL++CIWLHAGV H+ ANMLSLVFI
Sbjct: 159 ----------------LEKLGALEWSKVVEKKEGWRLLTCIWLHAGVIHLGANMLSLVFI 218

Query: 222 GIRLEQEFGFASYSYCRTVITLFCILVFLSIVRIGMLYIVSGFGGSLLSALFIQSSISVG 281
           GIRLEQ+FGF                     VRIG++Y++SG GGS+LS+LFI++SISVG
Sbjct: 219 GIRLEQQFGF---------------------VRIGVIYLLSGIGGSVLSSLFIRNSISVG 278

Query: 282 ASGALFGLLGGMLSELLTNWTIYANKLAALLTLLFIIVVNLAVGVLPHVDNFAHIGGFIS 341
           ASGALFGLLG MLSEL TNWTIY+NK+AALLTLLF+I++NLA+G+LPHVDNFAH+GGF++
Sbjct: 279 ASGALFGLLGSMLSELFTNWTIYSNKIAALLTLLFVILINLAIGILPHVDNFAHVGGFVT 338

Query: 342 GFLLGFVFLVRPQFGWVSQRNAPRGRSSTSKSKYKPYQYVLWIGSLLLLIAGFALGLVFL 401
           GFLLGF+ L RPQF W+++ + P+G  +  + KYK YQY+LW+ SL+LLIAGF + L+ L
Sbjct: 339 GFLLGFILLARPQFKWLAREHMPQG--TPLRYKYKTYQYLLWLLSLVLLIAGFVVALLML 344

Query: 402 FRGENLNDHCSWCHYLSCVPTSKWSCN 423
           FRGEN NDHC WCHYL CVPTS W C+
Sbjct: 399 FRGENGNDHCRWCHYLRCVPTSSWRCD 344

BLAST of Spg005884 vs. ExPASy Swiss-Prot
Match: Q8VZ48 (RHOMBOID-like protein 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL6 PE=2 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 3.3e-91
Identity = 178/371 (47.98%), Postives = 225/371 (60.65%), Query Frame = 0

Query: 55  KWVPWLVPVFVVANSVLFSISMYVNDCPKN----SASCVGRFLGRFSFQPLKENPLLGPS 114
           +W  WL P  VVAN  +F + MY NDCPK     +  CV + L RFSFQPL+ENP LGPS
Sbjct: 17  QWTAWLTPTIVVANVSIFIVVMYTNDCPKTTTGANGDCVAKLLRRFSFQPLRENPFLGPS 76

Query: 115 SSTDGNVFVLFECNLEMIMAALKYFFVDKMMGITKVVGVMKFWNYVGEFVCCMSDKCKKK 174
           SST                                                         
Sbjct: 77  SST--------------------------------------------------------- 136

Query: 175 RLEKMGALEVDKVVYGHQPWRLISCIWLHAGVFHILANMLSLVFIGIRLEQEFGFASYSY 234
            LEK+GAL+  KVV G++ WRLI+ +WLHAG+ H++ NM  ++  GIRLEQ+FGF     
Sbjct: 137 -LEKLGALDWKKVVQGNEKWRLITAMWLHAGIIHLVMNMFDVIIFGIRLEQQFGF----- 196

Query: 235 CRTVITLFCILVFLSIVRIGMLYIVSGFGGSLLSALFIQSSISVGASGALFGLLGGMLSE 294
                           +RIG++Y++SGFGGS+LSALF+Q SISVGASGAL GL+G MLSE
Sbjct: 197 ----------------IRIGLIYLISGFGGSILSALFLQKSISVGASGALLGLMGAMLSE 256

Query: 295 LLTNWTIYANKLAALLTLLFIIVVNLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFG 354
           LLTNWTIY +KL ALL+ LFII +NLA+G+LP VDNFAHIGG ++GF LGF+ L++PQ G
Sbjct: 257 LLTNWTIYKSKLCALLSFLFIIAINLAIGLLPWVDNFAHIGGLLTGFCLGFILLMQPQSG 307

Query: 355 WVSQRNAPRGRSSTSKSKYKPYQYVLWIGSLLLLIAGFALGLVFLFRGENLNDHCSWCHY 414
           W   RN+ +   + ++SKY P QYVL+  + +L++AG  +GLV LF GEN N HC WCH 
Sbjct: 317 WEEFRNSSQ-YGARARSKYNPCQYVLFFVAAVLVVAGLTVGLVMLFDGENGNKHCKWCHR 307

Query: 415 LSCVPTSKWSC 422
           L C PTSKWSC
Sbjct: 377 LDCYPTSKWSC 307

BLAST of Spg005884 vs. ExPASy TrEMBL
Match: A0A1S3C5Q5 (RHOMBOID-like protein OS=Cucumis melo OX=3656 GN=LOC103497154 PE=3 SV=1)

HSP 1 Score: 669.5 bits (1726), Expect = 1.0e-188
Identity = 352/469 (75.05%), Postives = 369/469 (78.68%), Query Frame = 0

Query: 1   MARDKPTAGFEIKVHPRHGEPEVHPAERDPLAAAARSRPSGSSPAGFGQFRPFKKWVPWL 60
           MAR+KPTAG EIKVH RHGE EV P       AAARSRPSG+SP  FGQ+RPFKKWVPWL
Sbjct: 1   MARNKPTAGIEIKVHSRHGEHEVQP-------AAARSRPSGTSPTEFGQYRPFKKWVPWL 60

Query: 61  VPVFVVANSVLFSISMYVNDCPKNSASCVGRFLGRFSFQPLKENPLLGPSSSTDGNVFVL 120
           VPVFVVANSV+F+ISMYVNDCPKNSASCVGRFLGRFSFQPLKENPLLGPSSST       
Sbjct: 61  VPVFVVANSVMFTISMYVNDCPKNSASCVGRFLGRFSFQPLKENPLLGPSSST------- 120

Query: 121 FECNLEMIMAALKYFFVDKMMGITKVVGVMKFWNYVGEFVCCMSDKCKKKRLEKMGALEV 180
                                                              LEKMGALEV
Sbjct: 121 ---------------------------------------------------LEKMGALEV 180

Query: 181 DKVVYGHQPWRLISCIWLHAGVFHILANMLSLVFIGIRLEQEFGFASYSYCRTVITLFCI 240
           DKVVYGHQPWRLISC+WLHAGVFHILANMLSL+FIGIRLEQEFGF               
Sbjct: 181 DKVVYGHQPWRLISCLWLHAGVFHILANMLSLIFIGIRLEQEFGF--------------- 240

Query: 241 LVFLSIVRIGMLYIVSGFGGSLLSALFIQSSISVGASGALFGLLGGMLSELLTNWTIYAN 300
                 VRIGMLYIVSGFGGSL+SALFIQS ISVGASGALFGLLGGMLSELLTNWTIYAN
Sbjct: 241 ------VRIGMLYIVSGFGGSLMSALFIQSGISVGASGALFGLLGGMLSELLTNWTIYAN 300

Query: 301 KLAALLTLLFIIVVNLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFGWVSQRNAPRG 360
           KLAALLTLLFIIV+NLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFGWVSQRNAPRG
Sbjct: 301 KLAALLTLLFIIVINLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFGWVSQRNAPRG 360

Query: 361 RSSTSKSKYKPYQYVLWIGSLLLLIAGFALGLVFLFRGENLNDHCSWCHYLSCVPTSKWS 420
             STSKSKYKPYQYVLW+ SL+LLIAGFA+GLVFLFRGEN+ND CSWCHYLSC+PTSKWS
Sbjct: 361 NRSTSKSKYKPYQYVLWVVSLVLLIAGFAVGLVFLFRGENMNDRCSWCHYLSCIPTSKWS 383

Query: 421 CNSQKFSCESSQLGNQLNITCLSNGRSGSYSLANSSSSEVQQICSQLCS 470
           CNSQKFSCE+SQLGNQLN+TCLSNGR GSYSL NSSSSE +QICSQLCS
Sbjct: 421 CNSQKFSCETSQLGNQLNMTCLSNGRRGSYSLTNSSSSEAEQICSQLCS 383

BLAST of Spg005884 vs. ExPASy TrEMBL
Match: A0A5D3BGU5 (RHOMBOID-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G002170 PE=3 SV=1)

HSP 1 Score: 669.5 bits (1726), Expect = 1.0e-188
Identity = 352/469 (75.05%), Postives = 369/469 (78.68%), Query Frame = 0

Query: 1   MARDKPTAGFEIKVHPRHGEPEVHPAERDPLAAAARSRPSGSSPAGFGQFRPFKKWVPWL 60
           MAR+KPTAG EIKVH RHGE EV P       AAARSRPSG+SP  FGQ+RPFKKWVPWL
Sbjct: 1   MARNKPTAGIEIKVHSRHGEHEVQP-------AAARSRPSGTSPTEFGQYRPFKKWVPWL 60

Query: 61  VPVFVVANSVLFSISMYVNDCPKNSASCVGRFLGRFSFQPLKENPLLGPSSSTDGNVFVL 120
           VPVFVVANSV+F+ISMYVNDCPKNSASCVGRFLGRFSFQPLKENPLLGPSSST       
Sbjct: 61  VPVFVVANSVMFTISMYVNDCPKNSASCVGRFLGRFSFQPLKENPLLGPSSST------- 120

Query: 121 FECNLEMIMAALKYFFVDKMMGITKVVGVMKFWNYVGEFVCCMSDKCKKKRLEKMGALEV 180
                                                              LEKMGALEV
Sbjct: 121 ---------------------------------------------------LEKMGALEV 180

Query: 181 DKVVYGHQPWRLISCIWLHAGVFHILANMLSLVFIGIRLEQEFGFASYSYCRTVITLFCI 240
           DKVVYGHQPWRLISC+WLHAGVFHILANMLSL+FIGIRLEQEFGF               
Sbjct: 181 DKVVYGHQPWRLISCLWLHAGVFHILANMLSLIFIGIRLEQEFGF--------------- 240

Query: 241 LVFLSIVRIGMLYIVSGFGGSLLSALFIQSSISVGASGALFGLLGGMLSELLTNWTIYAN 300
                 VRIGMLYIVSGFGGSL+SALFIQS ISVGASGALFGLLGGMLSELLTNWTIYAN
Sbjct: 241 ------VRIGMLYIVSGFGGSLMSALFIQSGISVGASGALFGLLGGMLSELLTNWTIYAN 300

Query: 301 KLAALLTLLFIIVVNLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFGWVSQRNAPRG 360
           KLAALLTLLFIIV+NLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFGWVSQRNAPRG
Sbjct: 301 KLAALLTLLFIIVINLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFGWVSQRNAPRG 360

Query: 361 RSSTSKSKYKPYQYVLWIGSLLLLIAGFALGLVFLFRGENLNDHCSWCHYLSCVPTSKWS 420
             STSKSKYKPYQYVLW+ SL+LLIAGFA+GLVFLFRGEN+ND CSWCHYLSC+PTSKWS
Sbjct: 361 NRSTSKSKYKPYQYVLWVVSLVLLIAGFAVGLVFLFRGENMNDRCSWCHYLSCIPTSKWS 383

Query: 421 CNSQKFSCESSQLGNQLNITCLSNGRSGSYSLANSSSSEVQQICSQLCS 470
           CNSQKFSCE+SQLGNQLN+TCLSNGR GSYSL NSSSSE +QICSQLCS
Sbjct: 421 CNSQKFSCETSQLGNQLNMTCLSNGRRGSYSLTNSSSSEAEQICSQLCS 383

BLAST of Spg005884 vs. ExPASy TrEMBL
Match: A0A0A0LXE7 (RHOMBOID-like protein OS=Cucumis sativus OX=3659 GN=Csa_1G523040 PE=3 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 1.4e-188
Identity = 352/469 (75.05%), Postives = 370/469 (78.89%), Query Frame = 0

Query: 1   MARDKPTAGFEIKVHPRHGEPEVHPAERDPLAAAARSRPSGSSPAGFGQFRPFKKWVPWL 60
           MARDKPTAG EIKVH RHGE EV P       AAARSRPSG+SP  FGQ+RPFKKWVPWL
Sbjct: 1   MARDKPTAGIEIKVHSRHGEHEVQP-------AAARSRPSGTSPIEFGQYRPFKKWVPWL 60

Query: 61  VPVFVVANSVLFSISMYVNDCPKNSASCVGRFLGRFSFQPLKENPLLGPSSSTDGNVFVL 120
           VPVFVVANSV+F+ISMYVNDCPKNSASC+GRFLGRFSFQPLKENPLLGPSSST       
Sbjct: 61  VPVFVVANSVMFTISMYVNDCPKNSASCIGRFLGRFSFQPLKENPLLGPSSST------- 120

Query: 121 FECNLEMIMAALKYFFVDKMMGITKVVGVMKFWNYVGEFVCCMSDKCKKKRLEKMGALEV 180
                                                              LEKMGALEV
Sbjct: 121 ---------------------------------------------------LEKMGALEV 180

Query: 181 DKVVYGHQPWRLISCIWLHAGVFHILANMLSLVFIGIRLEQEFGFASYSYCRTVITLFCI 240
           DKVVYGHQ WRLISC+WLHAGVFHILANMLSLVFIGIRLEQEFGF               
Sbjct: 181 DKVVYGHQAWRLISCLWLHAGVFHILANMLSLVFIGIRLEQEFGF--------------- 240

Query: 241 LVFLSIVRIGMLYIVSGFGGSLLSALFIQSSISVGASGALFGLLGGMLSELLTNWTIYAN 300
                 VRIGMLYIVSGFGGSL+SALFIQS ISVGASGALFGLLGGMLSELLTNWTIYAN
Sbjct: 241 ------VRIGMLYIVSGFGGSLMSALFIQSGISVGASGALFGLLGGMLSELLTNWTIYAN 300

Query: 301 KLAALLTLLFIIVVNLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFGWVSQRNAPRG 360
           KLAALLTLLFIIV+NLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFGWVSQRNAPRG
Sbjct: 301 KLAALLTLLFIIVINLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFGWVSQRNAPRG 360

Query: 361 RSSTSKSKYKPYQYVLWIGSLLLLIAGFALGLVFLFRGENLNDHCSWCHYLSCVPTSKWS 420
            SSTSKSKYKPYQYVLW+ SL+LLIAGFA+GLVFLFRGEN+ND CSWCHYLSC+PTSKWS
Sbjct: 361 NSSTSKSKYKPYQYVLWVVSLVLLIAGFAVGLVFLFRGENMNDRCSWCHYLSCIPTSKWS 383

Query: 421 CNSQKFSCESSQLGNQLNITCLSNGRSGSYSLANSSSSEVQQICSQLCS 470
           CNSQKFSCE+SQLGNQLN+TCLSNGRSGSYSL NSSS+E ++ICSQLCS
Sbjct: 421 CNSQKFSCETSQLGNQLNMTCLSNGRSGSYSLTNSSSTEAEKICSQLCS 383

BLAST of Spg005884 vs. ExPASy TrEMBL
Match: A0A6J1HNY8 (RHOMBOID-like protein OS=Cucurbita moschata OX=3662 GN=LOC111464687 PE=3 SV=1)

HSP 1 Score: 653.3 bits (1684), Expect = 7.8e-184
Identity = 345/469 (73.56%), Postives = 362/469 (77.19%), Query Frame = 0

Query: 1   MARDKPTAGFEIKVHPRHGEPEVHPAERDPLAAAARSRPSGSSPAGFGQFRPFKKWVPWL 60
           MARDKP A  EIKVH RHG+P V P+     AAAARSRPS +   GFGQFRPFKKW+PWL
Sbjct: 1   MARDKPAANIEIKVHSRHGDPAVEPS-----AAAARSRPSRTPSTGFGQFRPFKKWIPWL 60

Query: 61  VPVFVVANSVLFSISMYVNDCPKNSASCVGRFLGRFSFQPLKENPLLGPSSSTDGNVFVL 120
           VPVFVV NSVLFSI+MYVNDCPKNSASC+G FLGRFSFQPLKENPLLGPS ST       
Sbjct: 61  VPVFVVVNSVLFSITMYVNDCPKNSASCIGTFLGRFSFQPLKENPLLGPSPST------- 120

Query: 121 FECNLEMIMAALKYFFVDKMMGITKVVGVMKFWNYVGEFVCCMSDKCKKKRLEKMGALEV 180
                                                              LEKMGALEV
Sbjct: 121 ---------------------------------------------------LEKMGALEV 180

Query: 181 DKVVYGHQPWRLISCIWLHAGVFHILANMLSLVFIGIRLEQEFGFASYSYCRTVITLFCI 240
           DKVVYGHQPWRLISC+WLHAGVFHILANMLSLV IGIRLEQEFGF               
Sbjct: 181 DKVVYGHQPWRLISCLWLHAGVFHILANMLSLVCIGIRLEQEFGF--------------- 240

Query: 241 LVFLSIVRIGMLYIVSGFGGSLLSALFIQSSISVGASGALFGLLGGMLSELLTNWTIYAN 300
                 VRIGMLYIVSGFGGSLLSALFIQS ISVGASGALFGLLGGMLSELLTNWTIY N
Sbjct: 241 ------VRIGMLYIVSGFGGSLLSALFIQSGISVGASGALFGLLGGMLSELLTNWTIYEN 300

Query: 301 KLAALLTLLFIIVVNLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFGWVSQRNAPRG 360
           KLAALLTL+FIIV+NLAVGVLPHVDNFAHIGGFISGF LGFVFLVRPQFGWVSQ+NAPR 
Sbjct: 301 KLAALLTLVFIIVINLAVGVLPHVDNFAHIGGFISGFFLGFVFLVRPQFGWVSQQNAPRR 360

Query: 361 RSSTSKSKYKPYQYVLWIGSLLLLIAGFALGLVFLFRGENLNDHCSWCHYLSCVPTSKWS 420
            SSTSK+KY PYQYVLWI SLLLLIAGFA+GLVFLFRGENLND CSWCHYLSC+PTSKWS
Sbjct: 361 HSSTSKAKYMPYQYVLWIASLLLLIAGFAVGLVFLFRGENLNDRCSWCHYLSCIPTSKWS 385

Query: 421 CNSQKFSCESSQLGNQLNITCLSNGRSGSYSLANSSSSEVQQICSQLCS 470
           CNSQKFSCE+SQLGNQLN+TCLSNGRSGSYSLANS+SSE QQIC+QLCS
Sbjct: 421 CNSQKFSCETSQLGNQLNMTCLSNGRSGSYSLANSNSSEAQQICTQLCS 385

BLAST of Spg005884 vs. ExPASy TrEMBL
Match: A0A6J1HZE9 (RHOMBOID-like protein OS=Cucurbita maxima OX=3661 GN=LOC111469490 PE=3 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 2.5e-182
Identity = 341/469 (72.71%), Postives = 359/469 (76.55%), Query Frame = 0

Query: 1   MARDKPTAGFEIKVHPRHGEPEVHPAERDPLAAAARSRPSGSSPAGFGQFRPFKKWVPWL 60
           MARDKP A  EIKVH RHG+P V P+     AAAARSRPS +   GFGQFRPFKKW+PWL
Sbjct: 1   MARDKPAANIEIKVHSRHGDPAVEPS-----AAAARSRPSRTPSTGFGQFRPFKKWIPWL 60

Query: 61  VPVFVVANSVLFSISMYVNDCPKNSASCVGRFLGRFSFQPLKENPLLGPSSSTDGNVFVL 120
           VPVFVV NSVLFSI+MYVNDCPKNSASC+G FLGRFSFQP KENPLLGPS ST       
Sbjct: 61  VPVFVVVNSVLFSITMYVNDCPKNSASCIGTFLGRFSFQPFKENPLLGPSPST------- 120

Query: 121 FECNLEMIMAALKYFFVDKMMGITKVVGVMKFWNYVGEFVCCMSDKCKKKRLEKMGALEV 180
                                                              LEKMGALEV
Sbjct: 121 ---------------------------------------------------LEKMGALEV 180

Query: 181 DKVVYGHQPWRLISCIWLHAGVFHILANMLSLVFIGIRLEQEFGFASYSYCRTVITLFCI 240
           DKVVYGHQPWRLISC+WLHAGVFHILANMLSL+ IGIRLEQEFGF               
Sbjct: 181 DKVVYGHQPWRLISCLWLHAGVFHILANMLSLLCIGIRLEQEFGF--------------- 240

Query: 241 LVFLSIVRIGMLYIVSGFGGSLLSALFIQSSISVGASGALFGLLGGMLSELLTNWTIYAN 300
                 VRIGMLYI SGFGGSLLSALFIQS ISVGASGALFGLLGGMLSELLTNWTIY N
Sbjct: 241 ------VRIGMLYIASGFGGSLLSALFIQSGISVGASGALFGLLGGMLSELLTNWTIYEN 300

Query: 301 KLAALLTLLFIIVVNLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFGWVSQRNAPRG 360
           KLAALLTL+FIIV+NLA+GVLPHVDNFAHIGGFISGF LGFVFLVRPQFGWVSQRN PR 
Sbjct: 301 KLAALLTLVFIIVINLAMGVLPHVDNFAHIGGFISGFFLGFVFLVRPQFGWVSQRNEPRR 360

Query: 361 RSSTSKSKYKPYQYVLWIGSLLLLIAGFALGLVFLFRGENLNDHCSWCHYLSCVPTSKWS 420
            SSTSK+KYKPYQYVLWI S LLLIAGFA+GLVFLFRGENLND CSWCHYLSC+PTSKWS
Sbjct: 361 HSSTSKAKYKPYQYVLWIASFLLLIAGFAVGLVFLFRGENLNDRCSWCHYLSCIPTSKWS 385

Query: 421 CNSQKFSCESSQLGNQLNITCLSNGRSGSYSLANSSSSEVQQICSQLCS 470
           CNSQKFSCE+SQLGNQLN+TCLSNGRSGSYSLANS+SSE QQIC+QLCS
Sbjct: 421 CNSQKFSCETSQLGNQLNMTCLSNGRSGSYSLANSTSSEAQQICTQLCS 385

BLAST of Spg005884 vs. TAIR 10
Match: AT2G29050.1 (RHOMBOID-like 1 )

HSP 1 Score: 501.1 bits (1289), Expect = 9.5e-142
Identity = 269/470 (57.23%), Postives = 316/470 (67.23%), Query Frame = 0

Query: 1   MARDKPTAGFEIK-VHPRHGEPEVHPAERDPLAAAARSRPSGSSPAGFGQFRPFKKWVPW 60
           MARD+   G EIK V+P          E  P  A  R R      A F +FRPFK W PW
Sbjct: 1   MARDR-REGLEIKVVNPPAAATNNVAVETSPATATRRRRQ--QQRASFAEFRPFKLWFPW 60

Query: 61  LVPVFVVANSVLFSISMYVNDCPKNSASCVGRFLGRFSFQPLKENPLLGPSSSTDGNVFV 120
           LVP  VVAN  LF+ISM++N+CPKNSA C+ RFLGRF+FQP+KENPLLGPSS T      
Sbjct: 61  LVPAIVVANIALFAISMFINNCPKNSAYCLARFLGRFAFQPMKENPLLGPSSLT------ 120

Query: 121 LFECNLEMIMAALKYFFVDKMMGITKVVGVMKFWNYVGEFVCCMSDKCKKKRLEKMGALE 180
                                                               LEKMGAL+
Sbjct: 121 ----------------------------------------------------LEKMGALD 180

Query: 181 VDKVVYGHQPWRLISCIWLHAGVFHILANMLSLVFIGIRLEQEFGFASYSYCRTVITLFC 240
           V  VV+ H+ WRL +CIWLHAGVFH+LANMLSL+FIGIRLEQEFGF              
Sbjct: 181 VSMVVHKHEVWRLFTCIWLHAGVFHVLANMLSLIFIGIRLEQEFGF-------------- 240

Query: 241 ILVFLSIVRIGMLYIVSGFGGSLLSALFIQSSISVGASGALFGLLGGMLSELLTNWTIYA 300
                  VRIG+LY++SGFGGSLLS+LF ++ ISVGASGALFGLLG MLSELLTNWTIYA
Sbjct: 241 -------VRIGLLYMISGFGGSLLSSLFNRAGISVGASGALFGLLGAMLSELLTNWTIYA 300

Query: 301 NKLAALLTLLFIIVVNLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFGWVSQRNAPR 360
           NK AALLTL+FII +NLAVG+LPHVDNFAH+GGF SGFLLGFVFL+RPQ+G+ +QRN PR
Sbjct: 301 NKFAALLTLIFIIAINLAVGILPHVDNFAHLGGFTSGFLLGFVFLIRPQYGYFNQRNNPR 360

Query: 361 GRSSTS-KSKYKPYQYVLWIGSLLLLIAGFALGLVFLFRGENLNDHCSWCHYLSCVPTSK 420
           G ++ S KSK+KPYQYVLWI SL+LLIAG+  GLV L RG +LN HCSWCHYLSC+PTS 
Sbjct: 361 GYAAPSAKSKHKPYQYVLWITSLVLLIAGYTAGLVVLLRGTDLNKHCSWCHYLSCIPTSL 388

Query: 421 WSCNSQKFSCESSQLGNQLNITCLSNGRSGSYSLANSSSSEVQQICSQLC 469
           WSC SQ   CESSQ+G Q+N+TC++NG++  Y L+N   S +QQ+CSQLC
Sbjct: 421 WSCKSQNVYCESSQIGQQMNLTCITNGKTEMYKLSNDIPSRIQQLCSQLC 388

BLAST of Spg005884 vs. TAIR 10
Match: AT3G53780.2 (RHOMBOID-like protein 4 )

HSP 1 Score: 417.2 bits (1071), Expect = 1.8e-116
Identity = 214/418 (51.20%), Postives = 269/418 (64.35%), Query Frame = 0

Query: 51  RPFKKWVPWLVPVFVVANSVLFSISMYVNDCPKNSASCVGRFLGRFSFQPLKENPLLGPS 110
           + F+ W PWL+P FVVAN  +F I+MYVN+CPK S  C   FLGRFSFQ  +ENPLLGPS
Sbjct: 59  KEFRSWFPWLIPCFVVANVAVFVITMYVNNCPKKSGDCFADFLGRFSFQNTRENPLLGPS 118

Query: 111 SSTDGNVFVLFECNLEMIMAALKYFFVDKMMGITKVVGVMKFWNYVGEFVCCMSDKCKKK 170
           S T                                                         
Sbjct: 119 SLT--------------------------------------------------------- 178

Query: 171 RLEKMGALEVDKVVYGHQPWRLISCIWLHAGVFHILANMLSLVFIGIRLEQEFGFASYSY 230
            L+ MG L+V KVV G + WRL+SC WLH GV H+L NML+L+FIGIR+E+EFGF     
Sbjct: 179 -LQTMGGLDVKKVVKGDEGWRLLSCNWLHGGVVHLLMNMLTLLFIGIRMEREFGF----- 238

Query: 231 CRTVITLFCILVFLSIVRIGMLYIVSGFGGSLLSALFIQSSISVGASGALFGLLGGMLSE 290
                           +RIG+LY++SGFGGS+LSALF++S+ISVGASGA+FGLLGGMLSE
Sbjct: 239 ----------------IRIGLLYLISGFGGSILSALFLRSNISVGASGAVFGLLGGMLSE 298

Query: 291 LLTNWTIYANKLAALLTLLFIIVVNLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFG 350
           +  NWTIY+NK+  ++TL+ I+ VNL +GVLP VDNFAHIGGF +GFLLGFV L+RP +G
Sbjct: 299 IFINWTIYSNKVVTIVTLVLIVAVNLGLGVLPGVDNFAHIGGFATGFLLGFVLLIRPHYG 358

Query: 351 WVSQRNAPRGRSSTSKSKYKPYQYVLWIGSLLLLIAGFALGLVFLFRGENLNDHCSWCHY 410
           W++QRN P         ++K YQ +LW  SLL+L+AGF +GL+ LF   + N+HCSWCHY
Sbjct: 359 WINQRNGP----GAKPHRFKIYQGILWTISLLILVAGFIVGLISLFNNVDGNEHCSWCHY 393

Query: 411 LSCVPTSKWSCNSQKFSCESSQLGNQLNITCLSNGRSGSYSLANSSSSEVQQICSQLC 469
           LSCVPTSKWSCN +  SC ++QLGNQL++TCL NG+S SY LAN S S +  +C QLC
Sbjct: 419 LSCVPTSKWSCNREPASCTTTQLGNQLSMTCLRNGKSASYILANPSDSRINSLCVQLC 393

BLAST of Spg005884 vs. TAIR 10
Match: AT1G63120.1 (RHOMBOID-like 2 )

HSP 1 Score: 378.6 bits (971), Expect = 7.1e-105
Identity = 200/370 (54.05%), Postives = 237/370 (64.05%), Query Frame = 0

Query: 56  WVPWLVPVFVVANSVLFSISMYVNDCPKN----SASCVGRFLGRFSFQPLKENPLLGPSS 115
           W  WL+P  VVAN  +F   M+VNDCPK     +  CV RFLGRFSFQPLKENPL GPSS
Sbjct: 31  WTSWLIPAIVVANLAVFIAVMFVNDCPKKITGPNKECVARFLGRFSFQPLKENPLFGPSS 90

Query: 116 STDGNVFVLFECNLEMIMAALKYFFVDKMMGITKVVGVMKFWNYVGEFVCCMSDKCKKKR 175
           ST                                                          
Sbjct: 91  ST---------------------------------------------------------- 150

Query: 176 LEKMGALEVDKVVYGHQPWRLISCIWLHAGVFHILANMLSLVFIGIRLEQEFGFASYSYC 235
           LEKMGALE  KVV+ HQ WRL+SC+WLHAG+ H+L NMLSL+FIGIRLEQ+FGF      
Sbjct: 151 LEKMGALEWRKVVHEHQGWRLLSCMWLHAGIIHLLTNMLSLIFIGIRLEQQFGF------ 210

Query: 236 RTVITLFCILVFLSIVRIGMLYIVSGFGGSLLSALFIQSSISVGASGALFGLLGGMLSEL 295
                          +R+G++Y++SG GGS+LS+LF+Q SISVGASGALFGLLG MLSEL
Sbjct: 211 ---------------IRVGLIYLISGLGGSILSSLFLQESISVGASGALFGLLGAMLSEL 270

Query: 296 LTNWTIYANKLAALLTLLFIIVVNLAVGVLPHVDNFAHIGGFISGFLLGFVFLVRPQFGW 355
           LTNWTIYANK AAL+TLLFII +NLA+G+LP VDNFAHIGGF++GF LGFV LVRPQ+GW
Sbjct: 271 LTNWTIYANKAAALITLLFIIAINLALGMLPRVDNFAHIGGFLTGFCLGFVLLVRPQYGW 317

Query: 356 VSQRNAPRGRSSTSKSKYKPYQYVLWIGSLLLLIAGFALGLVFLFRGENLNDHCSWCHYL 415
                A R  +S +K KY  YQYVL++ S++LL+ G  + LV LF+GEN N HC WCHYL
Sbjct: 331 ----EASRTNTSRTKRKYSMYQYVLFVVSVVLLVVGLTVALVMLFKGENGNKHCKWCHYL 317

Query: 416 SCVPTSKWSC 422
           SC PTSKW+C
Sbjct: 391 SCFPTSKWTC 317

BLAST of Spg005884 vs. TAIR 10
Match: AT5G07250.1 (RHOMBOID-like protein 3 )

HSP 1 Score: 378.3 bits (970), Expect = 9.3e-105
Identity = 200/387 (51.68%), Postives = 249/387 (64.34%), Query Frame = 0

Query: 42  SSPAGFGQFRPFKKWVPWLVPVFVVANSVLFSISMYVNDCPKNSAS------CVGRFLGR 101
           SS   FG      +W  WLVP+FVVAN  +F ++M+VN+CP +  S      CV +FLGR
Sbjct: 39  SSSTEFGDNALSSRWTSWLVPMFVVANVAVFVVAMFVNNCPNHFESHRLRGHCVAKFLGR 98

Query: 102 FSFQPLKENPLLGPSSSTDGNVFVLFECNLEMIMAALKYFFVDKMMGITKVVGVMKFWNY 161
            SF+PL+ NPL GPSS T                                          
Sbjct: 99  LSFEPLRTNPLFGPSSHT------------------------------------------ 158

Query: 162 VGEFVCCMSDKCKKKRLEKMGALEVDKVVYGHQPWRLISCIWLHAGVFHILANMLSLVFI 221
                           LEK+GALE  KVV   + WRL++CIWLHAGV H+ ANMLSLVFI
Sbjct: 159 ----------------LEKLGALEWSKVVEKKEGWRLLTCIWLHAGVIHLGANMLSLVFI 218

Query: 222 GIRLEQEFGFASYSYCRTVITLFCILVFLSIVRIGMLYIVSGFGGSLLSALFIQSSISVG 281
           GIRLEQ+FGF                     VRIG++Y++SG GGS+LS+LFI++SISVG
Sbjct: 219 GIRLEQQFGF---------------------VRIGVIYLLSGIGGSVLSSLFIRNSISVG 278

Query: 282 ASGALFGLLGGMLSELLTNWTIYANKLAALLTLLFIIVVNLAVGVLPHVDNFAHIGGFIS 341
           ASGALFGLLG MLSEL TNWTIY+NK+AALLTLLF+I++NLA+G+LPHVDNFAH+GGF++
Sbjct: 279 ASGALFGLLGSMLSELFTNWTIYSNKIAALLTLLFVILINLAIGILPHVDNFAHVGGFVT 338

Query: 342 GFLLGFVFLVRPQFGWVSQRNAPRGRSSTSKSKYKPYQYVLWIGSLLLLIAGFALGLVFL 401
           GFLLGF+ L RPQF W+++ + P+G  +  + KYK YQY+LW+ SL+LLIAGF + L+ L
Sbjct: 339 GFLLGFILLARPQFKWLAREHMPQG--TPLRYKYKTYQYLLWLLSLVLLIAGFVVALLML 344

Query: 402 FRGENLNDHCSWCHYLSCVPTSKWSCN 423
           FRGEN NDHC WCHYL CVPTS W C+
Sbjct: 399 FRGENGNDHCRWCHYLRCVPTSSWRCD 344

BLAST of Spg005884 vs. TAIR 10
Match: AT2G29050.2 (RHOMBOID-like 1 )

HSP 1 Score: 376.7 bits (966), Expect = 2.7e-104
Identity = 220/418 (52.63%), Postives = 256/418 (61.24%), Query Frame = 0

Query: 1   MARDKPTAGFEIK-VHPRHGEPEVHPAERDPLAAAARSRPSGSSPAGFGQFRPFKKWVPW 60
           MARD+   G EIK V+P          E  P  A  R R      A F +FRPFK W PW
Sbjct: 1   MARDR-REGLEIKVVNPPAAATNNVAVETSPATATRRRRQ--QQRASFAEFRPFKLWFPW 60

Query: 61  LVPVFVVANSVLFSISMYVNDCPKNSASCVGRFLGRFSFQPLKENPLLGPSSSTDGNVFV 120
           LVP  VVAN  LF+ISM++N+CPKNSA C+ RFLGRF+FQP+KENPLLGPSS T      
Sbjct: 61  LVPAIVVANIALFAISMFINNCPKNSAYCLARFLGRFAFQPMKENPLLGPSSLT------ 120

Query: 121 LFECNLEMIMAALKYFFVDKMMGITKVVGVMKFWNYVGEFVCCMSDKCKKKRLEKMGALE 180
                                                               LEKMGAL+
Sbjct: 121 ----------------------------------------------------LEKMGALD 180

Query: 181 VDKVVYGHQPWRLISCIWLHAGVFHILANMLSLVFIGIRLEQEFGFASYSYCRTVITLFC 240
           V  VV+ H+ WRL +CIWLHAGVFH+LANMLSL+FIGIRLEQEFGF              
Sbjct: 181 VSMVVHKHEVWRLFTCIWLHAGVFHVLANMLSLIFIGIRLEQEFGF-------------- 240

Query: 241 ILVFLSIVRIGMLYIVSGFGGSLLSALFIQSSISVGASGALFGLLGGMLSELLTNWTIYA 300
                  VRIG+LY++SGFGGSLLS+LF ++ ISVGASGALFGLLG MLSELLTNWTIYA
Sbjct: 241 -------VRIGLLYMISGFGGSLLSSLFNRAGISVGASGALFGLLGAMLSELLTNWTIYA 300

Query: 301 NKL----------------------------AALLTLLFIIVVNLAVGVLPHVDNFAHIG 360
           NK+                            AALLTL+FII +NLAVG+LPHVDNFAH+G
Sbjct: 301 NKVAKSSLVKQAALSMNDVSIMSLVFLHLQFAALLTLIFIIAINLAVGILPHVDNFAHLG 336

Query: 361 GFISGFLLGFVFLVRPQFGWVSQRNAPRGRSSTS-KSKYKPYQYVLWIGSLLLLIAGF 389
           GF SGFLLGFVFL+RPQ+G+ +QRN PRG ++ S KSK+KPYQYVLWI SL+LLIAG+
Sbjct: 361 GFTSGFLLGFVFLIRPQYGYFNQRNNPRGYAAPSAKSKHKPYQYVLWITSLVLLIAGY 336

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895553.11.7e-18875.27RHOMBOID-like protein 1 [Benincasa hispida][more]
XP_008457471.12.2e-18875.05PREDICTED: RHOMBOID-like protein 1 [Cucumis melo] >XP_008457472.1 PREDICTED: RHO... [more]
XP_004153913.12.8e-18875.05RHOMBOID-like protein 1 isoform X1 [Cucumis sativus] >KGN65729.1 hypothetical pr... [more]
XP_022964679.11.6e-18373.56RHOMBOID-like protein 1 [Cucurbita moschata][more]
XP_023519656.13.6e-18373.35RHOMBOID-like protein 1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q0WQX71.3e-14057.23RHOMBOID-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RBL1 PE=2 SV=1[more]
F4JBM42.5e-11551.20RHOMBOID-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=RBL4 PE=2 SV=1[more]
Q9CAN11.0e-10354.05RHOMBOID-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RBL2 PE=1 SV=1[more]
Q9LYP11.3e-10351.68RHOMBOID-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RBL3 PE=2 SV=1[more]
Q8VZ483.3e-9147.98RHOMBOID-like protein 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL6 P... [more]
Match NameE-valueIdentityDescription
A0A1S3C5Q51.0e-18875.05RHOMBOID-like protein OS=Cucumis melo OX=3656 GN=LOC103497154 PE=3 SV=1[more]
A0A5D3BGU51.0e-18875.05RHOMBOID-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold19... [more]
A0A0A0LXE71.4e-18875.05RHOMBOID-like protein OS=Cucumis sativus OX=3659 GN=Csa_1G523040 PE=3 SV=1[more]
A0A6J1HNY87.8e-18473.56RHOMBOID-like protein OS=Cucurbita moschata OX=3662 GN=LOC111464687 PE=3 SV=1[more]
A0A6J1HZE92.5e-18272.71RHOMBOID-like protein OS=Cucurbita maxima OX=3661 GN=LOC111469490 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G29050.19.5e-14257.23RHOMBOID-like 1 [more]
AT3G53780.21.8e-11651.20RHOMBOID-like protein 4 [more]
AT1G63120.17.1e-10554.05RHOMBOID-like 2 [more]
AT5G07250.19.3e-10551.68RHOMBOID-like protein 3 [more]
AT2G29050.22.7e-10452.63RHOMBOID-like 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR035952Rhomboid-like superfamilyGENE3D1.20.1540.10coord: 143..346
e-value: 9.8E-34
score: 118.7
IPR035952Rhomboid-like superfamilySUPERFAMILY144091Rhomboid-likecoord: 56..348
IPR022764Peptidase S54, rhomboid domainPFAMPF01694Rhomboidcoord: 185..346
e-value: 1.3E-31
score: 109.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..45
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..31
NoneNo IPR availablePANTHERPTHR22936:SF77RHOMBOID-LIKE PROTEIN 1coord: 32..468
IPR002610Peptidase S54, rhomboidPANTHERPTHR22936RHOMBOID-RELATEDcoord: 32..468

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg005884.1Spg005884.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005794 Golgi apparatus
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004252 serine-type endopeptidase activity