Spg005834 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg005834
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like
Location	scaffold11: 1079222 .. 1082029 (+)
RNA-Seq Expression	Spg005834
Synteny	Spg005834

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ACCGGCTTGGTCGGAAAAGAGTTAGCCAAAGCCCTTCTCTCGACTGCAGGCTGGAAAGTCTACGGCGTCGCACGGCGGCCGGACAACGTCAGCCCCATCGCCGATCCCAATTTCCATTTCATCTCTTGCGATTTACTCGACCGCCGGTCGGCTCATCGGAGCCTCTCGCCGGTCCGCCACGTCACCCACCTGTTCTGGATCACTTGGGCCGCGCAGCTCCGGTTCGATTCGAGAGACTGCTCCGACCGGAACCGGGCCATGTTGGCCAACGCCCTCGACGTCATTCTCCCGGCGGCGCCGGCCCTTCGCCACGTCTCTCTACAGACAGGGCTCAAGCACTACGTTTCGTTGACGGGAGCCGCCGGGGGTGGCGGCGGAGGGGAGGTGGTTTATTGTGAGGATAGTCCGAGAGCGGAATCTGGGAATAATTTTTATTATGCGTTGGAGGATCTCCTCCGTGAGCGGCTGTCCGGCGGCGGCGGGCGGAGGGTGGGTTGGAGCGTTCTCCGGCCGGGTTTGATAGTGGGTTGTTCGAACCGGACTTTCTTTAATTTTATGGGGAGTTTGTGTGTTTATGGAGCAATTTGTAAGAAGTTGAAGATGCCGTTTGTGTTCGGCGGCACGGCGGCGTGCTGGGCGGAGGTTTGTATCGACGGCTCTGACGCTCGGCTCGTGGCGGAGCAGCACATCTGGGCCACCACGAAACAAATAAATGGTATGTTCAACTGTTGACTTCTTCGTTCTTTCTGAGTTGTCTTTATTCAATTTTTTTTTTTAGGAAATGGGTTTTTTCTTCTTTTTCACATTATGAAATATAACAAATTGGCTACCTCTACATATCCTATTAAAATTTTGACAACTAAGAAAGAGAAAGCGAGAAAATGATAAAACTTTTTTTATCTCTCCTAATTGTCAACATTTTAATGATGGTATGTAGGGAGATTCATACATATGGGTATGCAATCAAGTTTTTTCCTATAAAATATGTTCATGAATTTTATTTTTCATGTCTATATAGTTCTTTTTATAAAATATACTTCAACATAAGCATTGTTTGACATAGGTTGTTTTAATGTTTTTTTTTTTTGGGTAAAATAATGTTTTTACCTTGAGAAATTATCAGAGGAGACCATTACAATGTAACTATCAATTTTATCTTTACTAAATGTATAAGAGAGAAACCACCAAAAATAGTTTGAGAGAAACACTTAAAAAAGCAAATTTTCTAAAAAGAGTTTTTAAGTATATAATAAATTGTGAATAAAAACACTTACGAGAAAATGTAACTGAACATACAAGTGTTTAAATTCTAAACAATTTTTATACAAAATGTTTTTTAAAAAATGCATTCTTGAAAAATATTTAAACTAAAAGACATCCTAAACAAGCCCTAAATCAACATATCTTAGTATTAATTTAGAATATCTAATCTCTTGATCAATGATATAACTAGATGAACTGTGTTTAAGTTGGCAGTAACATTAGTAACTTTAATTTTTTTTTTAATAAATAAATCTTTAATACAAGTTAACCTCACATCATTTTAAAAAAATGTTATTGAAGTGACTAAATTAAACCATAAATGAGTAGTTTGAAAAATTTTCAACTAAACTATATGCTCTAGTATGGCATTTCACATCCATAAGGTGTGTTATTTTATTCAAAGGTTGTGTGTTTCTTCTTTTAGTTCAAAATGTAAACCAACATAGAGTATAAGAGTGTCACATGGTCAAAATCCAAATATGAATTTTTGGGGTGGTATCAATAGGCAACAACTTAGTTATATTTTGAATTGGAAAATATCTCAAATTTTCTAAAAAGTTTCCATCTCAAATGTTTCTAAAAAGTTGGTTCATCCAAGCAAAAACAAAATTATATCCTTGAAGAAAAGAGGAAGAAAGAGAAGATAGAACTAGGAAGATAGGATTTTTTTTTCTTTTGGAAAATACTTAGCTACATGCGGATACTTCCACGGCCATCAAATAAAAATGTTGACAACAAAGAGAGATAAAAATAATGAATCATACTTGGTTGTATAATGGTGGGTAGGGATCTCCTACATCCCATTAAAATTTTGACAAATAAGAGAGAAATGTAGAAAATGAAAATTTTATTTTATCTCTCCTAATTGTTGACGTTTTAATGATGGTATGTATGAACATCTCTACACACCGATATGCAGCTAAGTATTTTTTTTAAAAAAAAAAATCTCATTTTCTAATTTCACTCTAGTTAAATATATTTGTTTCTTTTCTGTTTTTTTTTTAAAAATGTATATATAAACACTAGCATTCACAATAAAAAATGGTTTTAAAAGACAATCTCATTGCATATGGAACGGCTAATAATTTGCCCATATTTATTCTCAATCTATAATTCTATATATAAAATTGAAATCTCTTATGTATAAAAATAGCTACCGCAAACGGGGAAGCATTCAATGTGTGCAACGGGTGGAGCTTCACGTGGAAGGAGATATGGGGGGCGGTGGCGGAGAAACTTGGAGTGGCGGCGGCGGAGGCGGAGGATGAGAGAATGTTTTCGCCGGAGTTCCATTACACGGCGGCGATGGGGGACAAAGGGAAAGTGTGGGCGGAGATAGTGGAGGAGGAAGGGCTGATTCCGACGGAAATGGAGGAGCTGGCGAATTGGGGGTTCTTGGACAGCTTGTTTAGGCTGCCGGAAAAAATGGTGGCCAGCCGGGAAAAGTCCGACCGATTTGGCTTCACTGTAAAATACAAGATGTTGGATTCCATCTTCTATTGGATTGATGTCATGAGACACGACAAATTGATTTCTTTTCCTTAA

mRNA sequence

ACCGGCTTGGTCGGAAAAGAGTTAGCCAAAGCCCTTCTCTCGACTGCAGGCTGGAAAGTCTACGGCGTCGCACGGCGGCCGGACAACGTCAGCCCCATCGCCGATCCCAATTTCCATTTCATCTCTTGCGATTTACTCGACCGCCGGTCGGCTCATCGGAGCCTCTCGCCGGTCCGCCACGTCACCCACCTGTTCTGGATCACTTGGGCCGCGCAGCTCCGGTTCGATTCGAGAGACTGCTCCGACCGGAACCGGGCCATGTTGGCCAACGCCCTCGACGTCATTCTCCCGGCGGCGCCGGCCCTTCGCCACGTCTCTCTACAGACAGGGCTCAAGCACTACGTTTCGTTGACGGGAGCCGCCGGGGGTGGCGGCGGAGGGGAGGTGGTTTATTGTGAGGATAGTCCGAGAGCGGAATCTGGGAATAATTTTTATTATGCGTTGGAGGATCTCCTCCGTGAGCGGCTGTCCGGCGGCGGCGGGCGGAGGGTGGGTTGGAGCGTTCTCCGGCCGGGTTTGATAGTGGGTTGTTCGAACCGGACTTTCTTTAATTTTATGGGGAGTTTGTGTGTTTATGGAGCAATTTGTAAGAAGTTGAAGATGCCGTTTGTGTTCGGCGGCACGGCGGCGTGCTGGGCGGAGGTTTGTATCGACGGCTCTGACGCTCGGCTCGTGGCGGAGCAGCACATCTGGGCCACCACGAAACAAATAAATGCTACCGCAAACGGGGAAGCATTCAATGTGTGCAACGGGTGGAGCTTCACGTGGAAGGAGATATGGGGGGCGGTGGCGGAGAAACTTGGAGTGGCGGCGGCGGAGGCGGAGGATGAGAGAATGTTTTCGCCGGAGTTCCATTACACGGCGGCGATGGGGGACAAAGGGAAAGTGTGGGCGGAGATAGTGGAGGAGGAAGGGCTGATTCCGACGGAAATGGAGGAGCTGGCGAATTGGGGGTTCTTGGACAGCTTGTTTAGGCTGCCGGAAAAAATGGTGGCCAGCCGGGAAAAGTCCGACCGATTTGGCTTCACTGTAAAATACAAGATGTTGGATTCCATCTTCTATTGGATTGATGTCATGAGACACGACAAATTGATTTCTTTTCCTTAA

Coding sequence (CDS)

ACCGGCTTGGTCGGAAAAGAGTTAGCCAAAGCCCTTCTCTCGACTGCAGGCTGGAAAGTCTACGGCGTCGCACGGCGGCCGGACAACGTCAGCCCCATCGCCGATCCCAATTTCCATTTCATCTCTTGCGATTTACTCGACCGCCGGTCGGCTCATCGGAGCCTCTCGCCGGTCCGCCACGTCACCCACCTGTTCTGGATCACTTGGGCCGCGCAGCTCCGGTTCGATTCGAGAGACTGCTCCGACCGGAACCGGGCCATGTTGGCCAACGCCCTCGACGTCATTCTCCCGGCGGCGCCGGCCCTTCGCCACGTCTCTCTACAGACAGGGCTCAAGCACTACGTTTCGTTGACGGGAGCCGCCGGGGGTGGCGGCGGAGGGGAGGTGGTTTATTGTGAGGATAGTCCGAGAGCGGAATCTGGGAATAATTTTTATTATGCGTTGGAGGATCTCCTCCGTGAGCGGCTGTCCGGCGGCGGCGGGCGGAGGGTGGGTTGGAGCGTTCTCCGGCCGGGTTTGATAGTGGGTTGTTCGAACCGGACTTTCTTTAATTTTATGGGGAGTTTGTGTGTTTATGGAGCAATTTGTAAGAAGTTGAAGATGCCGTTTGTGTTCGGCGGCACGGCGGCGTGCTGGGCGGAGGTTTGTATCGACGGCTCTGACGCTCGGCTCGTGGCGGAGCAGCACATCTGGGCCACCACGAAACAAATAAATGCTACCGCAAACGGGGAAGCATTCAATGTGTGCAACGGGTGGAGCTTCACGTGGAAGGAGATATGGGGGGCGGTGGCGGAGAAACTTGGAGTGGCGGCGGCGGAGGCGGAGGATGAGAGAATGTTTTCGCCGGAGTTCCATTACACGGCGGCGATGGGGGACAAAGGGAAAGTGTGGGCGGAGATAGTGGAGGAGGAAGGGCTGATTCCGACGGAAATGGAGGAGCTGGCGAATTGGGGGTTCTTGGACAGCTTGTTTAGGCTGCCGGAAAAAATGGTGGCCAGCCGGGAAAAGTCCGACCGATTTGGCTTCACTGTAAAATACAAGATGTTGGATTCCATCTTCTATTGGATTGATGTCATGAGACACGACAAATTGATTTCTTTTCCTTAA

Protein sequence

TGLVGKELAKALLSTAGWKVYGVARRPDNVSPIADPNFHFISCDLLDRRSAHRSLSPVRHVTHLFWITWAAQLRFDSRDCSDRNRAMLANALDVILPAAPALRHVSLQTGLKHYVSLTGAAGGGGGGEVVYCEDSPRAESGNNFYYALEDLLRERLSGGGGRRVGWSVLRPGLIVGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWAEVCIDGSDARLVAEQHIWATTKQINATANGEAFNVCNGWSFTWKEIWGAVAEKLGVAAAEAEDERMFSPEFHYTAAMGDKGKVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPEKMVASREKSDRFGFTVKYKMLDSIFYWIDVMRHDKLISFP

Homology

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BLAST of Spg005834 vs. NCBI nr
Match: XP_022998181.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita maxima])

HSP 1 Score: 669.8 bits (1727), Expect = 1.3e-188
Identity = 322/371 (86.79%), Postives = 338/371 (91.11%), Query Frame = 0


Query: 1   TGLVGKELAKALLSTAGWKVYGVARRPDNVSPIADPNFHFISCDLLDRRSAHRSLSPVRH 60
           TGLVGKELAK LLST GWKVYGVARRPDNVSPI D NF FISCDLLDR SAHR+LSPVRH
Sbjct: 24  TGLVGKELAKTLLSTGGWKVYGVARRPDNVSPITDTNFQFISCDLLDRSSAHRNLSPVRH 83

Query: 61  VTHLFWITWAAQLRFDSRDCSDRNRAMLANALDVILPAAPALRHVSLQTGLKHYVSLTGA 120
           VTHLFW+TW AQLRFD+ DCSD+NR MLAN LDVILPAAPALRHVSLQTG+KHYVSLTGA
Sbjct: 84  VTHLFWVTWTAQLRFDTSDCSDQNRTMLANVLDVILPAAPALRHVSLQTGIKHYVSLTGA 143

Query: 121 A--GGGGGGEVVYCEDSPRAESGNNFYYALEDLLRERLSGGGGRRVGWSVLRPGLIVGCS 180
           A  GGGGGG  VYCEDSPRAESGNNFYYALEDLLRERLS GGGRRV WSVLRPGLI+GCS
Sbjct: 144 AVDGGGGGGMAVYCEDSPRAESGNNFYYALEDLLRERLS-GGGRRVAWSVLRPGLILGCS 203

Query: 181 NRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWAEVCIDGSDARLVAEQHIWATTKQIN 240
           NRTFFNFMGSLCVYGAICKKLK PFVFGG+AACW   CI+GSDARLVAEQH+WA T +IN
Sbjct: 204 NRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVAEQHVWAATNEIN 263

Query: 241 ATANGEAFNVCNGWSFTWKEIWGAVAEKLGVAAAEAEDER-MFSPEFHYTAAMGDKGKVW 300
            TA+GEAFNVCNGWSFTWKEIWGAVAEK G+AAA AEDE+ MFSPEFHYTAAM +KGKVW
Sbjct: 264 TTADGEAFNVCNGWSFTWKEIWGAVAEKFGLAAAAAEDEKGMFSPEFHYTAAMAEKGKVW 323

Query: 301 AEIVEEEGLIPTEMEELANWGFLDSLFRLPEKMVASREKSDRFGFTVKYKMLDSIFYWID 360
           AEIVEEEGLIPTEMEELANWGFLDSLFRLP KMVASR KSDR GF+VKYKMLDSI YWID
Sbjct: 324 AEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSVKYKMLDSILYWID 383

Query: 361 VMRHDKLISFP 369
           VMR+D LI FP
Sbjct: 384 VMRYDTLIPFP 393

[Close]

BLAST of Spg005834 vs. NCBI nr
Match: XP_022948773.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita moschata])

HSP 1 Score: 668.7 bits (1724), Expect = 2.9e-188
Identity = 319/369 (86.45%), Postives = 339/369 (91.87%), Query Frame = 0


Query: 1   TGLVGKELAKALLSTAGWKVYGVARRPDNVSPIADPNFHFISCDLLDRRSAHRSLSPVRH 60
           TGLVGKELAKALLST GWKVYGVARRPDNVSPI D NF FISCDLLDR SAHR+LSPVRH
Sbjct: 24  TGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTNFQFISCDLLDRSSAHRNLSPVRH 83

Query: 61  VTHLFWITWAAQLRFDSRDCSDRNRAMLANALDVILPAAPALRHVSLQTGLKHYVSLTGA 120
           VTH+FW+TWAAQLRFD+ DCSD+NR MLAN LDVILPAAPALRHVSLQTG+KHYVSLTGA
Sbjct: 84  VTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAAPALRHVSLQTGIKHYVSLTGA 143

Query: 121 AGGGGGGEV-VYCEDSPRAESGNNFYYALEDLLRERLSGGGGRRVGWSVLRPGLIVGCSN 180
           A GGGGG + VYCEDSPRAESGNNFYYALEDLLRERLS GGGRRV WSVLRPGLI+GCSN
Sbjct: 144 AVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERLS-GGGRRVAWSVLRPGLILGCSN 203

Query: 181 RTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWAEVCIDGSDARLVAEQHIWATTKQINA 240
           RTFFNFMGSLCVYGAICKKLK PFVFGG+AACW   CI+GSDARLVAEQH+WA T +IN 
Sbjct: 204 RTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVAEQHVWAATNEINT 263

Query: 241 TANGEAFNVCNGWSFTWKEIWGAVAEKLGVAAAEAEDERMFSPEFHYTAAMGDKGKVWAE 300
           TA+GEAFNVCNGWSFTWKEIWGAVAEK GVAAA  ++E MFSPEFHYTAAM +KG++WAE
Sbjct: 264 TADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEEGMFSPEFHYTAAMTEKGRLWAE 323

Query: 301 IVEEEGLIPTEMEELANWGFLDSLFRLPEKMVASREKSDRFGFTVKYKMLDSIFYWIDVM 360
           IVEEEGLIPTEMEELANWGFLDSLFRLP KMVASR KSDR GF+VKYKMLDSI YWIDVM
Sbjct: 324 IVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSVKYKMLDSILYWIDVM 383

Query: 361 RHDKLISFP 369
           R+DKLI FP
Sbjct: 384 RYDKLIPFP 391

[Close]

BLAST of Spg005834 vs. NCBI nr
Match: KAG6607304.1 ((S)-8-oxocitronellyl enol synthase CYC2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 668.3 bits (1723), Expect = 3.8e-188
Identity = 320/369 (86.72%), Postives = 339/369 (91.87%), Query Frame = 0


Query: 1   TGLVGKELAKALLSTAGWKVYGVARRPDNVSPIADPNFHFISCDLLDRRSAHRSLSPVRH 60
           TGLVGKELAKALLST GWKVYGVARRPDNVSPI D NF FISCDLLDR SA+R+LSPVRH
Sbjct: 24  TGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTNFQFISCDLLDRSSANRNLSPVRH 83

Query: 61  VTHLFWITWAAQLRFDSRDCSDRNRAMLANALDVILPAAPALRHVSLQTGLKHYVSLTGA 120
           VTHLFW+TWAAQLRFD+ DCSD+NR MLAN LDVILPAAPALRHVSLQTG+KHYVSLTGA
Sbjct: 84  VTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAAPALRHVSLQTGIKHYVSLTGA 143

Query: 121 AGGGGGGEV-VYCEDSPRAESGNNFYYALEDLLRERLSGGGGRRVGWSVLRPGLIVGCSN 180
           A GGGGG + VYCEDSPRAESGNNFYYALEDLLRERLS GGGRRV WSVLRPGLI+GCSN
Sbjct: 144 AVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERLS-GGGRRVAWSVLRPGLILGCSN 203

Query: 181 RTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWAEVCIDGSDARLVAEQHIWATTKQINA 240
           RTFFNFMGSLCVYGAICKKLK PFVFGG+AACW   CI+GSDARLVAEQH+WA T +IN 
Sbjct: 204 RTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVAEQHVWAATNEINT 263

Query: 241 TANGEAFNVCNGWSFTWKEIWGAVAEKLGVAAAEAEDERMFSPEFHYTAAMGDKGKVWAE 300
           TA+GEAFNVCNGWSFTWKEIWGAVAEK GVAAA  ++E MFSPEFHYTAAM +KG+VWAE
Sbjct: 264 TADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEEGMFSPEFHYTAAMTEKGRVWAE 323

Query: 301 IVEEEGLIPTEMEELANWGFLDSLFRLPEKMVASREKSDRFGFTVKYKMLDSIFYWIDVM 360
           IVEEEGLIPTEMEELANWGFLDSLFRLP KMVASR KSDR GF+VKYKMLDSI YWIDVM
Sbjct: 324 IVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSVKYKMLDSILYWIDVM 383

Query: 361 RHDKLISFP 369
           R+DKLI FP
Sbjct: 384 RYDKLIPFP 391

[Close]

BLAST of Spg005834 vs. NCBI nr
Match: XP_038895390.1 ((S)-8-oxocitronellyl enol synthase CYC2 [Benincasa hispida])

HSP 1 Score: 667.9 bits (1722), Expect = 4.9e-188
Identity = 327/369 (88.62%), Postives = 342/369 (92.68%), Query Frame = 0


Query: 1   TGLVGKELAKALLSTAGWKVYGVARRPDNVSPIADPNFHFISCDLLDRRSAHRSLSPVRH 60
           TGLVGKELAK LLSTAGWKVYGVARRPDN+SPI+ PNFHFISCDLLDRRSAH +LSPVRH
Sbjct: 24  TGLVGKELAKTLLSTAGWKVYGVARRPDNISPISHPNFHFISCDLLDRRSAHSNLSPVRH 83

Query: 61  VTHLFWITWAAQLRFDSRDCSDRNRAMLANALDVILPAAPALRHVSLQTGLKHYVSLTG- 120
           VTHLFWITWAAQLRFDS+ CSD+NRAMLANALD ILP+APALRHVSLQTG+KHY SLT  
Sbjct: 84  VTHLFWITWAAQLRFDSQGCSDQNRAMLANALDAILPSAPALRHVSLQTGIKHYASLTRL 143

Query: 121 AAGGGGGGEVVYCEDSPRAESGNNFYYALEDLLRERLSGGGGRRVGWSVLRPGLIVGCSN 180
           AAG GGGGEV YCEDSPRAESGNNFYY LEDLLRERLS GG R VGWSVLRPGLI+G SN
Sbjct: 144 AAGCGGGGEVAYCEDSPRAESGNNFYYVLEDLLRERLS-GGRRVVGWSVLRPGLILGSSN 203

Query: 181 RTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWAEVCIDGSDARLVAEQHIWATTK--QI 240
           RTFFNFMGSLCVYGAICKKLKMPFVFGGTAACW EV IDGSDARL AEQH+WA TK  +I
Sbjct: 204 RTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAATKGIKI 263

Query: 241 NATANGEAFNVCNGWSFTWKEIWGAVAEKLGVAAAEAEDER-MFSPEFHYTAAMGDKGKV 300
           NATA+GEAFNVCNGWSF WKEIWGAVAEKLGVAAAE EDER MFS EFHYTAAMGDKGK+
Sbjct: 264 NATADGEAFNVCNGWSFRWKEIWGAVAEKLGVAAAEKEDERIMFSAEFHYTAAMGDKGKL 323

Query: 301 WAEIVEEEGLIPTEMEELANWGFLDSLFRLPEKMVASREKSDRFGFTVKYKMLDSIFYWI 360
           WAEIVEEEGL+PTEMEELANWGFLD+LFRLPEKMVASR KSDRFGFTVKYKMLDSI YWI
Sbjct: 324 WAEIVEEEGLVPTEMEELANWGFLDTLFRLPEKMVASRAKSDRFGFTVKYKMLDSILYWI 383

Query: 361 DVMRHDKLI 366
           DVMR+DKLI
Sbjct: 384 DVMRNDKLI 391

[Close]

BLAST of Spg005834 vs. NCBI nr
Match: KAG7036983.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 666.0 bits (1717), Expect = 1.9e-187
Identity = 319/369 (86.45%), Postives = 338/369 (91.60%), Query Frame = 0


Query: 1   TGLVGKELAKALLSTAGWKVYGVARRPDNVSPIADPNFHFISCDLLDRRSAHRSLSPVRH 60
           TGLVGKELAKALLST GWKVYGVARRPDNVSPI D NF FISCDLLDR SA+R+LSPVRH
Sbjct: 24  TGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTNFQFISCDLLDRSSANRNLSPVRH 83

Query: 61  VTHLFWITWAAQLRFDSRDCSDRNRAMLANALDVILPAAPALRHVSLQTGLKHYVSLTGA 120
           VTHLFW+TWAAQLRFD+ DCSD+NR MLAN LDVILPAAPALRHVSLQTG+KHYVSLTGA
Sbjct: 84  VTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAAPALRHVSLQTGIKHYVSLTGA 143

Query: 121 AGGGGGGEV-VYCEDSPRAESGNNFYYALEDLLRERLSGGGGRRVGWSVLRPGLIVGCSN 180
           A GGGGG + VYCEDSPRAESGNNFYYALEDLLRERLS GGGRRV WSVLRPGLI+GCSN
Sbjct: 144 AVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERLS-GGGRRVAWSVLRPGLILGCSN 203

Query: 181 RTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWAEVCIDGSDARLVAEQHIWATTKQINA 240
           RTFFNFMGSLCVYGAICKKLK PFVFGG+AACW   CI+GSDARLVAEQH+WA T +IN 
Sbjct: 204 RTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVAEQHVWAATNEINT 263

Query: 241 TANGEAFNVCNGWSFTWKEIWGAVAEKLGVAAAEAEDERMFSPEFHYTAAMGDKGKVWAE 300
           TA+GEAFNVCNGWSFTWKEIWGAVAEK GVAAA  ++E MFSPEFHY AAM +KG+VWAE
Sbjct: 264 TADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEEGMFSPEFHYMAAMTEKGRVWAE 323

Query: 301 IVEEEGLIPTEMEELANWGFLDSLFRLPEKMVASREKSDRFGFTVKYKMLDSIFYWIDVM 360
           IVEEEGLIPTEMEELANWGFLDSLFRLP KMVASR KSDR GF+VKYKMLDSI YWIDVM
Sbjct: 324 IVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSVKYKMLDSILYWIDVM 383

Query: 361 RHDKLISFP 369
           R+DKLI FP
Sbjct: 384 RYDKLIPFP 391

[Close]

BLAST of Spg005834 vs. ExPASy Swiss-Prot
Match: A0A1C9CX66 ((S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC2 PE=1 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 2.5e-62
Identity = 150/369 (40.65%), Postives = 194/369 (52.57%), Query Frame = 0


Query: 1   TGLVGKELAKAL--LSTAG--WKVYGVARRPDNVSPIADPNFHFISCDLLDRRSAHRSLS 60
           TG+VG  LA+ L    T G  WKVYGVARRP   +  AD    +I CD+ D    H  LS
Sbjct: 35  TGIVGNSLAEILPISDTPGGPWKVYGVARRP-RPAWNADHPVEYIQCDISDASDTHTKLS 94

Query: 61  PVRHVTHLFWITWAAQLRFDSRDCSDRNRAMLANALDVILPAAPALRHVSLQTGLKHYVS 120
           P+  VTH+FW+TWA   R    +C + N  ML N L+ ++P A  L H+ LQTG KHY+ 
Sbjct: 95  PLTDVTHIFWVTWAN--RPTESECCELNGTMLRNVLNALIPKAANLHHICLQTGHKHYIG 154

Query: 121 LTGAAGGGGGGEVVYCEDSPRAESGNNFYYALEDLLRERLSGGGGRRVGWSVLRPGLIVG 180
              A G     E  + ED PR  +  NFYY LED+L E      G  + WSV RP +I G
Sbjct: 155 PFEAFGKIKPHEPPFTEDMPRL-NAPNFYYTLEDMLVEASEKKAG--LNWSVHRPAVIFG 214

Query: 181 CSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWAEVCIDGSDARLVAEQHIWATTKQ 240
            S  +  N +G+LCVY AICK    P  F GT A W    +  SDA L+AE  IWA    
Sbjct: 215 FSPFSMMNIIGTLCVYAAICKHENTPLKFPGTKAAWNCYSV-ASDADLIAEHQIWAA--- 274

Query: 241 INATANGEAFNVCNGWSFTWKEIWGAVAEKLGVAAAEAEDERMFSPEFHYTAAMGDKGKV 300
           ++  A  EAFN  NG  F WK +W  +AE+ GV  AE ++             M DKG V
Sbjct: 275 VDPYAKNEAFNCSNGDLFKWKHLWKVLAEQFGVEYAEFDES---EKPTSLVERMKDKGPV 334

Query: 301 WAEIVEEEGLIPTEMEELANWGFLDSLFRLPEKMVASREKSDRFGFTVKYKMLDSIFYWI 360
           W EIV E GL  T++E +A W F D +    E ++ S  KS   G+       +S+   I
Sbjct: 335 WEEIVRENGLHTTKLEGVATWWFADVILG-GECLLDSMNKSKEHGYLGFRNTKNSLISVI 389

Query: 361 DVMRHDKLI 366
           D M+  K++
Sbjct: 395 DKMKAHKIV 389

[Close]

BLAST of Spg005834 vs. ExPASy Swiss-Prot
Match: Q6PQJ9 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 1.1e-57
Identity = 141/369 (38.21%), Postives = 192/369 (52.03%), Query Frame = 0


Query: 1   TGLVGKELAK--ALLSTAG--WKVYGVARRPDNVSPIADPNFHFISCDLLDRRSAHRSLS 60
           TG++G  LA+   L  T G  WKVYGVARR        +P  +++ CD+ D   +   LS
Sbjct: 35  TGIIGNSLAEILPLADTPGGPWKVYGVARRTRPAWHEDNP-INYVQCDISDPDDSQAKLS 94

Query: 61  PVRHVTHLFWITWAAQLRFDSRDCSDRNRAMLANALDVILPAAPALRHVSLQTGLKHYVS 120
           P+  VTH+F++TWA   R   ++  + N  M  N LD ++P  P L+H+SLQTG KHY+ 
Sbjct: 95  PLTDVTHVFYVTWAN--RSTEQENCEANSKMFRNVLDAVIPNCPNLKHISLQTGRKHYMG 154

Query: 121 LTGAAGGGGGGEVVYCEDSPRAESGNNFYYALEDLLRERLSGGGGRRVGWSVLRPGLIVG 180
              + G     +  Y ED PR +   NFYY LED++ E +    G  + WSV RPG I G
Sbjct: 155 PFESYGKIESHDPPYTEDLPRLKY-MNFYYDLEDIMLEEVEKKEG--LTWSVHRPGNIFG 214

Query: 181 CSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWAEVCIDGSDARLVAEQHIWATTKQ 240
            S  +  N +G+LCVY AICK       F G  A W +   D SDA L+AE HIWA    
Sbjct: 215 FSPYSMMNLVGTLCVYAAICKHEGKVLRFTGCKAAW-DGYSDCSDADLIAEHHIWAA--- 274

Query: 241 INATANGEAFNVCNGWSFTWKEIWGAVAEKLGVAAAEAEDERMFSPEFHYTAAMGDKGKV 300
           ++  A  EAFNV NG  F WK  W  +AE+ GV   E E+      +      M  K  V
Sbjct: 275 VDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGVGCGEYEE----GVDLKLQDLMKGKEPV 334

Query: 301 WAEIVEEEGLIPTEMEELANWGFLDSLFRLPEKMVASREKSDRFGFTVKYKMLDSIFYWI 360
           W EIV E GL PT+++++  W F D +    E  + S  KS   GF       ++   WI
Sbjct: 335 WEEIVRENGLTPTKLKDVGIWWFGDVILG-NECFLDSMNKSKEHGFLGFRNSKNAFISWI 388

Query: 361 DVMRHDKLI 366
           D  +  K++
Sbjct: 395 DKAKAYKIV 388

[Close]

BLAST of Spg005834 vs. ExPASy Swiss-Prot
Match: A0A221J5X1 ((S)-8-oxocitronellyl enol synthase ISY2 OS=Nepeta cataria OX=39347 GN=ISY2 PE=1 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 7.2e-57
Identity = 140/369 (37.94%), Postives = 190/369 (51.49%), Query Frame = 0


Query: 1   TGLVGKELAK--ALLSTAG--WKVYGVARRPDNVSPIADPNFHFISCDLLDRRSAHRSLS 60
           TGLVG  LA+   L  T G  WKVYGVARRP   S   D   ++ISCD+ +       L 
Sbjct: 36  TGLVGNSLAEMLPLSDTPGGPWKVYGVARRP-RPSWNEDHPINYISCDVSNTAEVEAKLP 95

Query: 61  PVRHVTHLFWITWAAQLRFDSRDCSDRNRAMLANALDVILPAAPALRHVSLQTGLKHYVS 120
           P+  VTH+F+ TW +  R    +  + N  ML N LD ++P  P L+H+ LQTG  HYV+
Sbjct: 96  PLSDVTHIFYATWTS--RSSEEENCEANGKMLKNVLDTMIPNCPNLKHICLQTGRFHYVA 155

Query: 121 LTGAAGGGGGGEVVYCEDSPRAESGNNFYYALEDLLRERLSGGGGRRVGWSVLRPGLIVG 180
                   G  +    ED PR  + NNFYY  ED+L E +    G  + WSV RPG I G
Sbjct: 156 SVVDWKINGSHDTPLTEDLPRLNT-NNFYYTQEDILLEEVKRKEG--LTWSVHRPGTIFG 215

Query: 181 CSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWAEVCIDGSDARLVAEQHIWATTKQ 240
            S  +  N +G+LCVY AICK+      F G    W +   D +DA L+AE +IWA    
Sbjct: 216 FSPYSMMNLVGTLCVYAAICKQEGAVLRFPGCKGAW-DGYSDCADADLIAEHYIWAA--- 275

Query: 241 INATANGEAFNVCNGWSFTWKEIWGAVAEKLGVAAAEAEDERMFSPEFHYTAAMGDKGKV 300
           ++  A  ++FNV NG  F WK +W  +AE+ GV     E E     +      M DKG V
Sbjct: 276 LDPHAKNQSFNVSNGDVFKWKHLWKVLAEQFGVECGGYEYEE--GQQVRLQDVMKDKGPV 335

Query: 301 WAEIVEEEGLIPTEMEELANWGFLDSLFRLPEKMVASREKSDRFGFTVKYKMLDSIFYWI 360
           W +IV E GL  T++E++  W F D++    E  + S  KS   GF       +   YWI
Sbjct: 336 WDKIVRENGLSNTKLEDVGKWWFSDTIL-WNECRLDSMNKSKEHGFLGFRNSKNCFLYWI 391

Query: 361 DVMRHDKLI 366
             ++  K++
Sbjct: 396 HKLKAYKIV 391

[Close]

BLAST of Spg005834 vs. ExPASy Swiss-Prot
Match: Q9STX2 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP1 PE=1 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.6e-56
Identity = 142/370 (38.38%), Postives = 194/370 (52.43%), Query Frame = 0


Query: 1   TGLVGKELAK--ALLSTAG--WKVYGVARRPDNVSPIADPNFHFISCDLLDRRSAHRSLS 60
           TG+VG  LA+   L  T G  WKVYGVARRP   +  AD    +I CD+ D       LS
Sbjct: 35  TGIVGNSLAEILPLSDTPGGPWKVYGVARRP-RPTWNADHPIDYIQCDVSDAEDTRSKLS 94

Query: 61  PVRHVTHLFWITWAAQLRFDSRDCSDRNRAMLANALDVILPAAPALRHVSLQTGLKHYVS 120
           P+  VTH+F++TW  +   +S +C + N +ML N L  I+P AP LRHV LQTG KHY+ 
Sbjct: 95  PLTDVTHVFYVTWTNR-ESESENC-EANGSMLRNVLQAIIPYAPNLRHVCLQTGTKHYLG 154

Query: 121 -LTGAAGGGGGGEVVYCEDSPRAESGNNFYYALEDLLRERLSGGGGRRVGWSVLRPGLIV 180
             T     G   +  + ED PR +   NFYY  ED+L E +       V WS+ RP +I 
Sbjct: 155 PFTNV--DGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIK--KIETVTWSIHRPNMIF 214

Query: 181 GCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWAEVCIDGSDARLVAEQHIWATTK 240
           G S  +  N +G+LCVY AICK    P +F G+   W E  +  SDA L+AEQ IWA   
Sbjct: 215 GFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAW-EGFMTASDADLIAEQQIWAA-- 274

Query: 241 QINATANGEAFNVCNGWSFTWKEIWGAVAEKLGVAAAEAEDERMFSPEFHYTAAMGDKGK 300
            ++  A  EAFN  N   F WK +W  +AE+ G+     E+ +           M  K +
Sbjct: 275 -VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEEGK----NLGLVEMMKGKER 334

Query: 301 VWAEIVEEEGLIPTEMEELANWGFLDSLFRLPEKMVASREKSDRFGFTVKYKMLDSIFYW 360
           VW E+V+E  L   ++EE+  W F D +  + E M+ S  KS  +GF       +S   W
Sbjct: 335 VWEEMVKENQLQEKKLEEVGVWWFADVILGV-EGMIDSMNKSKEYGFLGFRNSNNSFISW 387

Query: 361 IDVMRHDKLI 366
           ID  +  K++
Sbjct: 395 IDKYKAFKIV 387

[Close]

BLAST of Spg005834 vs. ExPASy Swiss-Prot
Match: A0A221J5X6 ((S)-8-oxocitronellyl enol synthase ISY2 OS=Nepeta racemosa OX=54731 GN=ISY2 PE=1 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 4.7e-56
Identity = 140/369 (37.94%), Postives = 190/369 (51.49%), Query Frame = 0


Query: 1   TGLVGKELAK--ALLSTAG--WKVYGVARRPDNVSPIADPNFHFISCDLLDRRSAHRSLS 60
           TGLVG  LA+   L  T G  WKVYGVARR    S   D    +ISCD+ +       LS
Sbjct: 38  TGLVGNSLAEMLPLPDTPGGPWKVYGVARRA-RPSWNEDQPMTYISCDVSNTGEVEAKLS 97

Query: 61  PVRHVTHLFWITWAAQLRFDSRDCSDRNRAMLANALDVILPAAPALRHVSLQTGLKHYVS 120
           P+  VTH+F+ TW +  R    +  + N  ML N LD ++P  P L+H+ LQTG  HYV+
Sbjct: 98  PLSDVTHIFYATWTS--RSTEEENCEANGKMLKNVLDAMIPNCPNLKHICLQTGRFHYVA 157

Query: 121 LTGAAGGGGGGEVVYCEDSPRAESGNNFYYALEDLLRERLSGGGGRRVGWSVLRPGLIVG 180
                   G  +    ED PR ++ NNFYY  ED+L E +    G  + WSV RPG I G
Sbjct: 158 SVVDWKINGSHDTPLTEDLPRLKT-NNFYYTQEDILLEEVKRKEG--LTWSVHRPGTIFG 217

Query: 181 CSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWAEVCIDGSDARLVAEQHIWATTKQ 240
            S  +  N +G+LCVY AICK+      F G    W +   D +DA L+AEQ IWA    
Sbjct: 218 FSPYSMMNLVGTLCVYAAICKQEGAVLRFPGCKGAW-DGHSDCADADLIAEQQIWAA--- 277

Query: 241 INATANGEAFNVCNGWSFTWKEIWGAVAEKLGVAAAEAEDERMFSPEFHYTAAMGDKGKV 300
           ++  A  +AFNV NG  F WK +W  +A++ GV   + E+      +      M DKG V
Sbjct: 278 LDPHAKNQAFNVSNGDLFKWKHLWKVLADQFGVECGDYEE----GQQLRLQDVMKDKGPV 337

Query: 301 WAEIVEEEGLIPTEMEELANWGFLDSLFRLPEKMVASREKSDRFGFTVKYKMLDSIFYWI 360
           W +IV E GL  T++E++  W F D++    E  + S  KS   GF       +   YWI
Sbjct: 338 WDKIVAENGLSNTKLEDVGKWWFSDTIL-WNECRLDSMNKSKEHGFLGFRNSKNCFLYWI 391

Query: 361 DVMRHDKLI 366
             ++   L+
Sbjct: 398 HKVKAYNLV 391

[Close]

BLAST of Spg005834 vs. ExPASy TrEMBL
Match: A0A6J1K9J8 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111492905 PE=3 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 6.3e-189
Identity = 322/371 (86.79%), Postives = 338/371 (91.11%), Query Frame = 0


Query: 1   TGLVGKELAKALLSTAGWKVYGVARRPDNVSPIADPNFHFISCDLLDRRSAHRSLSPVRH 60
           TGLVGKELAK LLST GWKVYGVARRPDNVSPI D NF FISCDLLDR SAHR+LSPVRH
Sbjct: 24  TGLVGKELAKTLLSTGGWKVYGVARRPDNVSPITDTNFQFISCDLLDRSSAHRNLSPVRH 83

Query: 61  VTHLFWITWAAQLRFDSRDCSDRNRAMLANALDVILPAAPALRHVSLQTGLKHYVSLTGA 120
           VTHLFW+TW AQLRFD+ DCSD+NR MLAN LDVILPAAPALRHVSLQTG+KHYVSLTGA
Sbjct: 84  VTHLFWVTWTAQLRFDTSDCSDQNRTMLANVLDVILPAAPALRHVSLQTGIKHYVSLTGA 143

Query: 121 A--GGGGGGEVVYCEDSPRAESGNNFYYALEDLLRERLSGGGGRRVGWSVLRPGLIVGCS 180
           A  GGGGGG  VYCEDSPRAESGNNFYYALEDLLRERLS GGGRRV WSVLRPGLI+GCS
Sbjct: 144 AVDGGGGGGMAVYCEDSPRAESGNNFYYALEDLLRERLS-GGGRRVAWSVLRPGLILGCS 203

Query: 181 NRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWAEVCIDGSDARLVAEQHIWATTKQIN 240
           NRTFFNFMGSLCVYGAICKKLK PFVFGG+AACW   CI+GSDARLVAEQH+WA T +IN
Sbjct: 204 NRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVAEQHVWAATNEIN 263

Query: 241 ATANGEAFNVCNGWSFTWKEIWGAVAEKLGVAAAEAEDER-MFSPEFHYTAAMGDKGKVW 300
            TA+GEAFNVCNGWSFTWKEIWGAVAEK G+AAA AEDE+ MFSPEFHYTAAM +KGKVW
Sbjct: 264 TTADGEAFNVCNGWSFTWKEIWGAVAEKFGLAAAAAEDEKGMFSPEFHYTAAMAEKGKVW 323

Query: 301 AEIVEEEGLIPTEMEELANWGFLDSLFRLPEKMVASREKSDRFGFTVKYKMLDSIFYWID 360
           AEIVEEEGLIPTEMEELANWGFLDSLFRLP KMVASR KSDR GF+VKYKMLDSI YWID
Sbjct: 324 AEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSVKYKMLDSILYWID 383

Query: 361 VMRHDKLISFP 369
           VMR+D LI FP
Sbjct: 384 VMRYDTLIPFP 393

[Close]

BLAST of Spg005834 vs. ExPASy TrEMBL
Match: A0A6J1GA93 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111452342 PE=3 SV=1)

HSP 1 Score: 668.7 bits (1724), Expect = 1.4e-188
Identity = 319/369 (86.45%), Postives = 339/369 (91.87%), Query Frame = 0


Query: 1   TGLVGKELAKALLSTAGWKVYGVARRPDNVSPIADPNFHFISCDLLDRRSAHRSLSPVRH 60
           TGLVGKELAKALLST GWKVYGVARRPDNVSPI D NF FISCDLLDR SAHR+LSPVRH
Sbjct: 24  TGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTNFQFISCDLLDRSSAHRNLSPVRH 83

Query: 61  VTHLFWITWAAQLRFDSRDCSDRNRAMLANALDVILPAAPALRHVSLQTGLKHYVSLTGA 120
           VTH+FW+TWAAQLRFD+ DCSD+NR MLAN LDVILPAAPALRHVSLQTG+KHYVSLTGA
Sbjct: 84  VTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAAPALRHVSLQTGIKHYVSLTGA 143

Query: 121 AGGGGGGEV-VYCEDSPRAESGNNFYYALEDLLRERLSGGGGRRVGWSVLRPGLIVGCSN 180
           A GGGGG + VYCEDSPRAESGNNFYYALEDLLRERLS GGGRRV WSVLRPGLI+GCSN
Sbjct: 144 AVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERLS-GGGRRVAWSVLRPGLILGCSN 203

Query: 181 RTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWAEVCIDGSDARLVAEQHIWATTKQINA 240
           RTFFNFMGSLCVYGAICKKLK PFVFGG+AACW   CI+GSDARLVAEQH+WA T +IN 
Sbjct: 204 RTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVAEQHVWAATNEINT 263

Query: 241 TANGEAFNVCNGWSFTWKEIWGAVAEKLGVAAAEAEDERMFSPEFHYTAAMGDKGKVWAE 300
           TA+GEAFNVCNGWSFTWKEIWGAVAEK GVAAA  ++E MFSPEFHYTAAM +KG++WAE
Sbjct: 264 TADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEEGMFSPEFHYTAAMTEKGRLWAE 323

Query: 301 IVEEEGLIPTEMEELANWGFLDSLFRLPEKMVASREKSDRFGFTVKYKMLDSIFYWIDVM 360
           IVEEEGLIPTEMEELANWGFLDSLFRLP KMVASR KSDR GF+VKYKMLDSI YWIDVM
Sbjct: 324 IVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSVKYKMLDSILYWIDVM 383

Query: 361 RHDKLISFP 369
           R+DKLI FP
Sbjct: 384 RYDKLIPFP 391

[Close]

BLAST of Spg005834 vs. ExPASy TrEMBL
Match: A0A0A0LVI2 (Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G530150 PE=3 SV=1)

HSP 1 Score: 645.6 bits (1664), Expect = 1.3e-181
Identity = 318/372 (85.48%), Postives = 332/372 (89.25%), Query Frame = 0


Query: 1   TGLVGKELAKALLSTAGWKVYGVARRPDNVSPIADPNFHFISCDLLDRRSAHRSLSPVRH 60
           TGLVGK+LAK LLSTAGWKVYGVARRPDNVSPI+ P FHFISCDLLDRRS  ++LSPVRH
Sbjct: 24  TGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPKFHFISCDLLDRRSVQQNLSPVRH 83

Query: 61  VTHLFWITWAAQLRFDSRDCSDRNRAMLANALDVILPAAPALRHVSLQTGLKHYVSLTGA 120
           VTHLFWITWAAQLR DS DCSD+NRAMLANALD ILP+APALRHVSLQTG+KHY SLT  
Sbjct: 84  VTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILPSAPALRHVSLQTGIKHYASLTRL 143

Query: 121 A---GGGGGGEVVYCEDSPRAESGNNFYYALEDLLRERLSGGGGRRVGWSVLRPGLIVGC 180
           A   GGGG GEVVYCEDSPRAESGNNFYY LEDLLRERLS  G R V WSVLRPGLI+GC
Sbjct: 144 AAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRERLS-CGRRMVAWSVLRPGLILGC 203

Query: 181 SNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWAEVCIDGSDARLVAEQHIWATTK-- 240
           SNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACW EV IDGSDARL AEQHIW  TK  
Sbjct: 204 SNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAEQHIWVATKAV 263

Query: 241 QINATANGEAFNVCNGWSFTWKEIWGAVAEKLGVA--AAEAEDERMFSPEFHYTAAMGDK 300
           +INATA+GEAFNVCNGWSF WKEIWGAVAEKLGVA   AE E ERMFS EFHYTAAMGDK
Sbjct: 264 EINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTGAEEEKERMFSKEFHYTAAMGDK 323

Query: 301 GKVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPEKMVASREKSDRFGFTVKYKMLDSIF 360
           GKVWAEIVEEEGL+ TEMEELANWGFLD+LFRLPEKMVASR KSDR GF VKYKMLDS+ 
Sbjct: 324 GKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMVASRAKSDRLGFNVKYKMLDSML 383

Query: 361 YWIDVMRHDKLI 366
           YWIDVMR+DKLI
Sbjct: 384 YWIDVMRNDKLI 394

[Close]

BLAST of Spg005834 vs. ExPASy TrEMBL
Match: A0A6J1HM54 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111464842 PE=3 SV=1)

HSP 1 Score: 642.1 bits (1655), Expect = 1.4e-180
Identity = 312/370 (84.32%), Postives = 331/370 (89.46%), Query Frame = 0


Query: 1   TGLVGKELAKALLSTAGWKVYGVARRPDNVSPIADPNFHFISCDLLDRRSAHRSLSPVRH 60
           TGLVGKELAK LLSTAGWKVYGVARRPDN+SPI+DPNFHFISCDLLDRRSAH +LSP+ H
Sbjct: 24  TGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPNFHFISCDLLDRRSAHLNLSPLGH 83

Query: 61  VTHLFWITWAAQLRFDSRDCSDRNRAMLANALDVILPAAPALRHVSLQTGLKHYVSLTGA 120
           VTH+FWITWAAQLRFDS+DCSD+N+AMLANALD ILP+APALRHVSLQTG+KHY SLT +
Sbjct: 84  VTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILPSAPALRHVSLQTGVKHYASLTSS 143

Query: 121 AGGGGGGEVVYCEDSPRAESGNNFYYALEDLLRERLSGGGGRRVGWSVLRPGLIVGCSNR 180
             G GGGEVVY E+SPRAESGNNFYY LEDLLRERLS G  R V WS+LRPGLI+GCSNR
Sbjct: 144 T-GSGGGEVVYSENSPRAESGNNFYYVLEDLLRERLS-GRRRVVAWSILRPGLILGCSNR 203

Query: 181 TFFNFMGSLCVYGAICKKLKMPFVFGGTAACWAEVCIDGSDARLVAEQHIWATTK--QIN 240
           TFFNFMGSLCVYGAICKKLKMPFVFGGTAACW EV ID SDARL AEQH+WA TK  +IN
Sbjct: 204 TFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDSSDARLTAEQHVWAATKAVEIN 263

Query: 241 ATANGEAFNVCNGWSFTWKEIWGAVAEKLGVAAAEAEDERMFSPEFHYTAAMGDKGKVWA 300
           A  +GEAFNVCNGWSFTWKEIWGAVAEKLGV    AEDERMFSPEF YTAAM +KG VWA
Sbjct: 264 ALTDGEAFNVCNGWSFTWKEIWGAVAEKLGVTVG-AEDERMFSPEFRYTAAMAEKGTVWA 323

Query: 301 EIVEEEGLIPTEMEELANWGFLDSLFRLPEKMVASREKSDRFGFTVKYKMLDSIFYWIDV 360
           EIVEEEGL+ TEM ELANWGFLDSLFRLPEKMVASR KSDR GFTVKYKMLDSI YWIDV
Sbjct: 324 EIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDV 383

Query: 361 MRHDKLISFP 369
           MRHDKLI  P
Sbjct: 384 MRHDKLIPSP 390

[Close]

BLAST of Spg005834 vs. ExPASy TrEMBL
Match: A0A6J1I0N6 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111469437 PE=3 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 3.1e-180
Identity = 312/370 (84.32%), Postives = 330/370 (89.19%), Query Frame = 0


Query: 1   TGLVGKELAKALLSTAGWKVYGVARRPDNVSPIADPNFHFISCDLLDRRSAHRSLSPVRH 60
           TGLVGKELAK LLSTAGWKVYGVARRPDN+SPI+DPNFHFISCDLLDRRSAH +LSPVRH
Sbjct: 24  TGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPNFHFISCDLLDRRSAHLNLSPVRH 83

Query: 61  VTHLFWITWAAQLRFDSRDCSDRNRAMLANALDVILPAAPALRHVSLQTGLKHYVSLTGA 120
           VTH+FWITWAAQLRFDS+DCSD+N+AMLANALD ILP+A ALRHVSLQTG+KHY SLT +
Sbjct: 84  VTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILPSASALRHVSLQTGVKHYASLTSS 143

Query: 121 AGGGGGGEVVYCEDSPRAESGNNFYYALEDLLRERLSGGGGRRVGWSVLRPGLIVGCSNR 180
               GGGEVVY EDSPRAESGNNFYY LEDLLRERLS G  R V WS+LRPGLI+GCSNR
Sbjct: 144 T-SSGGGEVVYSEDSPRAESGNNFYYVLEDLLRERLS-GYRRVVAWSILRPGLILGCSNR 203

Query: 181 TFFNFMGSLCVYGAICKKLKMPFVFGGTAACWAEVCIDGSDARLVAEQHIWATTK--QIN 240
           TFFNFMGSLCVYGAICKKLKMPFVFGGTAACW EV IDGSDARL AEQH+WA T+  +IN
Sbjct: 204 TFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDGSDARLTAEQHVWAATQAVEIN 263

Query: 241 ATANGEAFNVCNGWSFTWKEIWGAVAEKLGVAAAEAEDERMFSPEFHYTAAMGDKGKVWA 300
           A  + EAFNVCNGWSFTWKEIWGAVAEKLGV    AEDERMFSPEFHYT AM +KG VWA
Sbjct: 264 AITDREAFNVCNGWSFTWKEIWGAVAEKLGVTVG-AEDERMFSPEFHYTEAMAEKGMVWA 323

Query: 301 EIVEEEGLIPTEMEELANWGFLDSLFRLPEKMVASREKSDRFGFTVKYKMLDSIFYWIDV 360
           EIVEEEGL+ TEM ELANWGFLDSLFRLPEKMVASR KSDR GFTVKYKMLDSI YWIDV
Sbjct: 324 EIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKSDRLGFTVKYKMLDSILYWIDV 383

Query: 361 MRHDKLISFP 369
           MRHDKLI  P
Sbjct: 384 MRHDKLIPSP 390

[Close]

BLAST of Spg005834 vs. TAIR 10
Match: AT5G58750.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 384.8 bits (987), Expect = 7.8e-107
Identity = 197/369 (53.39%), Postives = 249/369 (67.48%), Query Frame = 0


Query: 1   TGLVGKELAKALL-STAGWKVYGVARRPDNVSPIADPNFHFISCDLLDRRSAHRSLSPVR 60
           TGLVG+E+ K LL S  GW++YGVAR P+  S      ++FISCDLL+     + LSP++
Sbjct: 25  TGLVGREIVKTLLMSKPGWRIYGVARNPEINS--MTKMYNFISCDLLNASETKQRLSPLQ 84

Query: 61  H-VTHLFWITWAAQLRFDSRDCSDRNRAMLANALDVILPAAPALRHVSLQTGLKHYVSLT 120
             V+H+FW+TW+ +   D+ +C  +N+ ML NALD ILP A  L+H SLQTG+KHYVSL 
Sbjct: 85  DIVSHVFWVTWSGEFPLDTDECCVQNKTMLMNALDAILPNAKRLKHFSLQTGMKHYVSLV 144

Query: 121 --GAAGGGGGGEVVYCEDSPRAESGNNFYYALEDLLRERLSGGGGRRVGWSVLRPGLIVG 180
               A G G     Y E+ PR  SG NFYY LEDLL+E+++      V WSV RPGL++G
Sbjct: 145 EETMARGEGSSLYYYSEECPRKSSGKNFYYVLEDLLKEKITRSS---VVWSVQRPGLLMG 204

Query: 181 CSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWAEVCIDGSDARLVAEQHIWATTKQ 240
            S+RT +NFMGSLCVYGA+CK L +PFVFGGT  CW E  IDGSD+ LVAEQHI+A T  
Sbjct: 205 SSSRTLYNFMGSLCVYGAMCKYLNLPFVFGGTRECWEESYIDGSDSNLVAEQHIFAATSG 264

Query: 241 INATANGEAFNVCNGWSFTWKEIWGAVAEKLGVAAAEAEDERMFSPEFHYTAAMGDKGKV 300
                 GEAFN  NG  FTWKEIW  + +KLGV   +  +  MF   F +   M ++  V
Sbjct: 265 -KVREKGEAFNAINGVGFTWKEIWPEIGKKLGV---QVNETTMFDEGFWFGREMVERKHV 324

Query: 301 WAEIVEEEGLIPTEMEELANWGFLDSLFRLPEKMVASREKSDRFGFTVKYKMLDSIFYWI 360
           W EIV +E L+ TE+E+LANW FLD+LFR P K++  REK DRFGF  KY+ LDS+ YWI
Sbjct: 325 WDEIVVKEKLVRTEIEDLANWYFLDALFRCPFKLLGKREKVDRFGFKRKYRTLDSVLYWI 384

Query: 361 DVMRHDKLI 366
           DVMR +KLI
Sbjct: 385 DVMRDEKLI 384

[Close]

BLAST of Spg005834 vs. TAIR 10
Match: AT4G24220.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 221.5 bits (563), Expect = 1.1e-57
Identity = 142/370 (38.38%), Postives = 194/370 (52.43%), Query Frame = 0


Query: 1   TGLVGKELAK--ALLSTAG--WKVYGVARRPDNVSPIADPNFHFISCDLLDRRSAHRSLS 60
           TG+VG  LA+   L  T G  WKVYGVARRP   +  AD    +I CD+ D       LS
Sbjct: 35  TGIVGNSLAEILPLSDTPGGPWKVYGVARRP-RPTWNADHPIDYIQCDVSDAEDTRSKLS 94

Query: 61  PVRHVTHLFWITWAAQLRFDSRDCSDRNRAMLANALDVILPAAPALRHVSLQTGLKHYVS 120
           P+  VTH+F++TW  +   +S +C + N +ML N L  I+P AP LRHV LQTG KHY+ 
Sbjct: 95  PLTDVTHVFYVTWTNR-ESESENC-EANGSMLRNVLQAIIPYAPNLRHVCLQTGTKHYLG 154

Query: 121 -LTGAAGGGGGGEVVYCEDSPRAESGNNFYYALEDLLRERLSGGGGRRVGWSVLRPGLIV 180
             T     G   +  + ED PR +   NFYY  ED+L E +       V WS+ RP +I 
Sbjct: 155 PFTNV--DGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIK--KIETVTWSIHRPNMIF 214

Query: 181 GCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWAEVCIDGSDARLVAEQHIWATTK 240
           G S  +  N +G+LCVY AICK    P +F G+   W E  +  SDA L+AEQ IWA   
Sbjct: 215 GFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAW-EGFMTASDADLIAEQQIWAA-- 274

Query: 241 QINATANGEAFNVCNGWSFTWKEIWGAVAEKLGVAAAEAEDERMFSPEFHYTAAMGDKGK 300
            ++  A  EAFN  N   F WK +W  +AE+ G+     E+ +           M  K +
Sbjct: 275 -VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEEGK----NLGLVEMMKGKER 334

Query: 301 VWAEIVEEEGLIPTEMEELANWGFLDSLFRLPEKMVASREKSDRFGFTVKYKMLDSIFYW 360
           VW E+V+E  L   ++EE+  W F D +  + E M+ S  KS  +GF       +S   W
Sbjct: 335 VWEEMVKENQLQEKKLEEVGVWWFADVILGV-EGMIDSMNKSKEYGFLGFRNSNNSFISW 387

Query: 361 IDVMRHDKLI 366
           ID  +  K++
Sbjct: 395 IDKYKAFKIV 387

[Close]

BLAST of Spg005834 vs. TAIR 10
Match: AT4G24220.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 221.5 bits (563), Expect = 1.1e-57
Identity = 142/370 (38.38%), Postives = 194/370 (52.43%), Query Frame = 0


Query: 1   TGLVGKELAK--ALLSTAG--WKVYGVARRPDNVSPIADPNFHFISCDLLDRRSAHRSLS 60
           TG+VG  LA+   L  T G  WKVYGVARRP   +  AD    +I CD+ D       LS
Sbjct: 34  TGIVGNSLAEILPLSDTPGGPWKVYGVARRP-RPTWNADHPIDYIQCDVSDAEDTRSKLS 93

Query: 61  PVRHVTHLFWITWAAQLRFDSRDCSDRNRAMLANALDVILPAAPALRHVSLQTGLKHYVS 120
           P+  VTH+F++TW  +   +S +C + N +ML N L  I+P AP LRHV LQTG KHY+ 
Sbjct: 94  PLTDVTHVFYVTWTNR-ESESENC-EANGSMLRNVLQAIIPYAPNLRHVCLQTGTKHYLG 153

Query: 121 -LTGAAGGGGGGEVVYCEDSPRAESGNNFYYALEDLLRERLSGGGGRRVGWSVLRPGLIV 180
             T     G   +  + ED PR +   NFYY  ED+L E +       V WS+ RP +I 
Sbjct: 154 PFTNV--DGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIK--KIETVTWSIHRPNMIF 213

Query: 181 GCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWAEVCIDGSDARLVAEQHIWATTK 240
           G S  +  N +G+LCVY AICK    P +F G+   W E  +  SDA L+AEQ IWA   
Sbjct: 214 GFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAW-EGFMTASDADLIAEQQIWAA-- 273

Query: 241 QINATANGEAFNVCNGWSFTWKEIWGAVAEKLGVAAAEAEDERMFSPEFHYTAAMGDKGK 300
            ++  A  EAFN  N   F WK +W  +AE+ G+     E+ +           M  K +
Sbjct: 274 -VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEEGK----NLGLVEMMKGKER 333

Query: 301 VWAEIVEEEGLIPTEMEELANWGFLDSLFRLPEKMVASREKSDRFGFTVKYKMLDSIFYW 360
           VW E+V+E  L   ++EE+  W F D +  + E M+ S  KS  +GF       +S   W
Sbjct: 334 VWEEMVKENQLQEKKLEEVGVWWFADVILGV-EGMIDSMNKSKEYGFLGFRNSNNSFISW 386

Query: 361 IDVMRHDKLI 366
           ID  +  K++
Sbjct: 394 IDKYKAFKIV 386

The following BLAST results are available for this feature:

BLAST of Spg005834 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022998181.1	1.3e-188	86.79	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita maxima]	[more]
XP_022948773.1	2.9e-188	86.45	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita moschata]	[more]
KAG6607304.1	3.8e-188	86.72	(S)-8-oxocitronellyl enol synthase CYC2, partial [Cucurbita argyrosperma subsp. ...	[more]
XP_038895390.1	4.9e-188	88.62	(S)-8-oxocitronellyl enol synthase CYC2 [Benincasa hispida]	[more]
KAG7036983.1	1.9e-187	86.45	3-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp...	[more]

BLAST of Spg005834 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A1C9CX66	2.5e-62	40.65	(S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC...	[more]
Q6PQJ9	1.1e-57	38.21	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1	[more]
A0A221J5X1	7.2e-57	37.94	(S)-8-oxocitronellyl enol synthase ISY2 OS=Nepeta cataria OX=39347 GN=ISY2 PE=1 ...	[more]
Q9STX2	1.6e-56	38.38	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP...	[more]
A0A221J5X6	4.7e-56	37.94	(S)-8-oxocitronellyl enol synthase ISY2 OS=Nepeta racemosa OX=54731 GN=ISY2 PE=1...	[more]

BLAST of Spg005834 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K9J8	6.3e-189	86.79	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1GA93	1.4e-188	86.45	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A0A0LVI2	1.3e-181	85.48	Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G530150 P...	[more]
A0A6J1HM54	1.4e-180	84.32	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1I0N6	3.1e-180	84.32	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]

BLAST of Spg005834 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G58750.1	7.8e-107	53.39	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G24220.1	1.1e-57	38.38	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G24220.2	1.1e-57	38.38	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001509	NAD-dependent epimerase/dehydratase	PFAM	PF01370	Epimerase	coord: 1..64 e-value: 2.0E-4 score: 20.9
None	No IPR available	GENE3D	3.40.50.720		coord: 1..365 e-value: 1.7E-96 score: 325.3
None	No IPR available	PANTHER	PTHR32487	3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE	coord: 1..365
None	No IPR available	PANTHER	PTHR32487:SF12	3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE ISOFORM X1	coord: 1..365
None	No IPR available	CDD	cd08948	5beta-POR_like_SDR_a	coord: 1..325 e-value: 2.40245E-92 score: 277.204
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 1..273

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg005834.1	Spg005834.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003824	catalytic activity