Spg005773 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg005773 vs. NCBI nr
Match: TYJ97368.1 (metal tolerance protein B [Cucumis melo var. makuwa])

HSP 1 Score: 588.6 bits (1516), Expect = 4.6e-164
Identity = 310/374 (82.89%), Postives = 332/374 (88.77%), Query Frame = 0

Query: 42  EEEMPILRTEHWHEVVKTPISAKKRNISIPNSPDVKCCSSSCAFSRLEHSNLESLNRSKS 101
           EEE+ IL+TEHW E VK PI AKKRN+ IP S +VKCC+S CAFSRLEHSNLESL RSKS
Sbjct: 3   EEEVLILKTEHWDE-VKMPIVAKKRNVVIPTSSEVKCCNSGCAFSRLEHSNLESLKRSKS 62

Query: 102 TMKLGGLIFFYAIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGWDATP 161
            MKLGG+I FY IAIVVEIIGGLRANSL+VMTDAAHLLSDVAGFSVSLFAVWVSGW+ATP
Sbjct: 63  AMKLGGMILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATP 122

Query: 162 QHSFGYNRLEVLGALVSVQLIWLISGVLIYEAVDRILGQKTKVNGFIMFAIAAFGFILNL 221
           QHSFGYNRLEVLGALVSVQLIWLISGVLIYEA+DRIL QKTKV+GF+MFA+AAFGFILNL
Sbjct: 123 QHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAVAAFGFILNL 182

Query: 222 FMVMWLGHSHSHSHGHSHSHSCHHSHHSH----QNHLDHDTDHEKEEIYTMTKQEGVPLG 281
           FMV+WLGHSH H H HS SH CHH HHSH    QNHL H++DHEKEEIYT+TK EG  LG
Sbjct: 183 FMVIWLGHSHHHHHSHS-SHCCHHDHHSHSHSDQNHLGHESDHEKEEIYTLTKLEGASLG 242

Query: 282 PKDNGSMLNINLQGAYLHVITDMIQSIGVMIAGAVLWVKPEWTVVDLICTLVFSVLAMAT 341
            KDN S LNINLQGAYLHVITDMIQSIGVMIAG VLW KPEW VVDLICTLVFSVLA+AT
Sbjct: 243 SKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGFVLWFKPEWIVVDLICTLVFSVLALAT 302

Query: 342 TFSMLRHTAGILMEGTPREVNIESLENDLKGIKGVLALHDLHIWSITVGKVVLSCHVVAE 401
           TFSMLRH A ILMEGTPREV+IESLEND+K IKGV  LHDLHIWSITVGKVVLSCHVVAE
Sbjct: 303 TFSMLRHIAVILMEGTPREVHIESLENDIKNIKGVHDLHDLHIWSITVGKVVLSCHVVAE 362

Query: 402 HGVPAREIISKIKN 412
            GV +RE+I KIK+
Sbjct: 363 AGVCSRELILKIKS 374

BLAST of Spg005773 vs. NCBI nr
Match: XP_008457438.1 (PREDICTED: metal tolerance protein B [Cucumis melo] >XP_008457439.1 PREDICTED: metal tolerance protein B [Cucumis melo] >XP_008457440.1 PREDICTED: metal tolerance protein B [Cucumis melo])

HSP 1 Score: 588.6 bits (1516), Expect = 4.6e-164
Identity = 310/374 (82.89%), Postives = 332/374 (88.77%), Query Frame = 0

Query: 42  EEEMPILRTEHWHEVVKTPISAKKRNISIPNSPDVKCCSSSCAFSRLEHSNLESLNRSKS 101
           EEE+ IL+TEHW E VK PI AKKRN+ IP S +VKCC+S CAFSRLEHSNLESL RSKS
Sbjct: 3   EEEVLILKTEHWDE-VKMPIVAKKRNVVIPTSSEVKCCNSGCAFSRLEHSNLESLKRSKS 62

Query: 102 TMKLGGLIFFYAIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGWDATP 161
            MKLGG+I FY IAIVVEIIGGLRANSL+VMTDAAHLLSDVAGFSVSLFAVWVSGW+ATP
Sbjct: 63  AMKLGGMILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATP 122

Query: 162 QHSFGYNRLEVLGALVSVQLIWLISGVLIYEAVDRILGQKTKVNGFIMFAIAAFGFILNL 221
           QHSFGYNRLEVLGALVSVQLIWLISGVLIYEA+DRIL QKTKV+GF+MFA+AAFGFILNL
Sbjct: 123 QHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAVAAFGFILNL 182

Query: 222 FMVMWLGHSHSHSHGHSHSHSCHHSHHSH----QNHLDHDTDHEKEEIYTMTKQEGVPLG 281
           FMV+WLGHSH H H HS SH CHH HHSH    QNHL H++DHEKEEIYT+TK EG  LG
Sbjct: 183 FMVIWLGHSHHHHHSHS-SHCCHHDHHSHSHSDQNHLGHESDHEKEEIYTLTKLEGASLG 242

Query: 282 PKDNGSMLNINLQGAYLHVITDMIQSIGVMIAGAVLWVKPEWTVVDLICTLVFSVLAMAT 341
            KDN S LNINLQGAYLHVITDMIQSIGVMIAG VLW KPEW VVDLICTLVFSVLA+AT
Sbjct: 243 SKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGFVLWFKPEWIVVDLICTLVFSVLALAT 302

Query: 342 TFSMLRHTAGILMEGTPREVNIESLENDLKGIKGVLALHDLHIWSITVGKVVLSCHVVAE 401
           TFSMLRH A ILMEGTPREV+IESLEND+K IKGV  LHDLHIWSITVGKVVLSCHVVAE
Sbjct: 303 TFSMLRHIAVILMEGTPREVHIESLENDIKNIKGVHDLHDLHIWSITVGKVVLSCHVVAE 362

Query: 402 HGVPAREIISKIKN 412
            GV +RE+I KIK+
Sbjct: 363 AGVCSRELILKIKS 374

BLAST of Spg005773 vs. NCBI nr
Match: KAA0031763.1 (metal tolerance protein B [Cucumis melo var. makuwa])

HSP 1 Score: 584.3 bits (1505), Expect = 8.7e-163
Identity = 307/374 (82.09%), Postives = 330/374 (88.24%), Query Frame = 0

Query: 42  EEEMPILRTEHWHEVVKTPISAKKRNISIPNSPDVKCCSSSCAFSRLEHSNLESLNRSKS 101
           EEE+ IL+TEHW E VK PI AKKRN+ IP S +VKCC+S CAFSRLEHSNLESL RSKS
Sbjct: 3   EEEVLILKTEHWDE-VKMPIVAKKRNVVIPTSSEVKCCNSGCAFSRLEHSNLESLKRSKS 62

Query: 102 TMKLGGLIFFYAIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGWDATP 161
            MKLGG+I FY IAIVVEIIGG RANSL+VMTDAAHLLSDVAGFSVSLFAVWVSGW+ TP
Sbjct: 63  AMKLGGMILFYTIAIVVEIIGGFRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEVTP 122

Query: 162 QHSFGYNRLEVLGALVSVQLIWLISGVLIYEAVDRILGQKTKVNGFIMFAIAAFGFILNL 221
           QHSFGYNRLEVLGALVSVQLIWLISGVLIYEA+DRIL QKTKV+GF+MFA+AAFGFILNL
Sbjct: 123 QHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAVAAFGFILNL 182

Query: 222 FMVMWLGHSHSHSHGHSHSHSCHHSHHSH----QNHLDHDTDHEKEEIYTMTKQEGVPLG 281
           FMV+WLGHSH H H HS SH CHH HHSH    QNHL H++DHEKEEIYT+TK EG  LG
Sbjct: 183 FMVIWLGHSHHHHHSHS-SHCCHHDHHSHSHSDQNHLGHESDHEKEEIYTLTKLEGASLG 242

Query: 282 PKDNGSMLNINLQGAYLHVITDMIQSIGVMIAGAVLWVKPEWTVVDLICTLVFSVLAMAT 341
            KDN S LNINLQGAYLHVITD+IQSIGVMIAG VLW KPEW VVDLICTLVFSVLA+AT
Sbjct: 243 SKDNSSTLNINLQGAYLHVITDLIQSIGVMIAGFVLWFKPEWIVVDLICTLVFSVLALAT 302

Query: 342 TFSMLRHTAGILMEGTPREVNIESLENDLKGIKGVLALHDLHIWSITVGKVVLSCHVVAE 401
           TFSMLRH A ILMEGTPREV+IESLEND+K IKGV  LHDLHIWSITVGKVVLSCHVVAE
Sbjct: 303 TFSMLRHIAVILMEGTPREVHIESLENDIKNIKGVHDLHDLHIWSITVGKVVLSCHVVAE 362

Query: 402 HGVPAREIISKIKN 412
            GV +RE+I KIK+
Sbjct: 363 AGVCSRELILKIKS 374

BLAST of Spg005773 vs. NCBI nr
Match: XP_038893887.1 (metal tolerance protein B [Benincasa hispida] >XP_038893888.1 metal tolerance protein B [Benincasa hispida])

HSP 1 Score: 570.5 bits (1469), Expect = 1.3e-158
Identity = 304/380 (80.00%), Postives = 332/380 (87.37%), Query Frame = 0

Query: 42  EEEMPILRTEHWHEVVKTPISAKKRNIS---IPNSPDVKCCSSSCAFSRLEHSNLESLNR 101
           EEE+ IL+TE+W EV+  PI  KKRN+S   IPNSP+VKCCSS CAFSRLEH NLESL R
Sbjct: 3   EEEVLILKTENWDEVM-MPIVPKKRNVSIPIIPNSPEVKCCSSGCAFSRLEHCNLESLKR 62

Query: 102 SKSTMKLGGLIFFYAIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGWD 161
           SKS MKLGGLI FY IAIVVEIIGGLRANSLAVMTDA HLLSDVAGF+ SLFAVWVSGW+
Sbjct: 63  SKSAMKLGGLILFYTIAIVVEIIGGLRANSLAVMTDAVHLLSDVAGFAASLFAVWVSGWE 122

Query: 162 ATPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAVDRILGQKTKVNGFIMFAIAAFGFI 221
           ATPQHSFGYNRLEVLGALVSVQLIWLISGVLIY+A++RILGQKTKV+GF+MFAIAAFGFI
Sbjct: 123 ATPQHSFGYNRLEVLGALVSVQLIWLISGVLIYQAINRILGQKTKVDGFLMFAIAAFGFI 182

Query: 222 LNLFMVMWLGHSH-SHSHGHSHSHSCHHSH--HSHQNHLDHDTDHEKEEIYTMTKQEGVP 281
           LNLFMV+WLGHSH SHS     SH CHH H  HSHQ+HLDH++DHE EEIYT+TKQEG  
Sbjct: 183 LNLFMVIWLGHSHDSHS-----SHCCHHDHHSHSHQHHLDHESDHENEEIYTLTKQEGGS 242

Query: 282 LGPKDNGSMLNINLQGAYLHVITDMIQSIGVMIAGAVLWVKPEWTVVDLICTLVFSVLAM 341
           L  + N S L+IN+QGAYLHVITDMIQSIGVMIAG +LW KP+W VVDLICTLVFSVL++
Sbjct: 243 LASQGNSSTLDINIQGAYLHVITDMIQSIGVMIAGVILWFKPDWIVVDLICTLVFSVLSL 302

Query: 342 ATTFSMLRHTAGILMEGTPREVNIESLENDLKGIKGVLALHDLHIWSITVGKVVLSCHVV 401
           ATTFSMLRHTAGILMEGTP+EVNIE LENDLK IKGV  LHDLHIWSITVGKVVLSCHVV
Sbjct: 303 ATTFSMLRHTAGILMEGTPKEVNIEILENDLKNIKGVHDLHDLHIWSITVGKVVLSCHVV 362

Query: 402 AEHGVPAREIISKIKNLSLK 416
           AE GV  RE+I KI+NL  K
Sbjct: 363 AEPGVCTRELILKIRNLCEK 376

BLAST of Spg005773 vs. NCBI nr
Match: NP_001295861.1 (metal tolerance protein B [Cucumis sativus] >XP_004145281.2 metal tolerance protein B isoform X1 [Cucumis sativus] >XP_011658317.2 metal tolerance protein B isoform X1 [Cucumis sativus] >AFJ24701.1 metal transport protein 4 [Cucumis sativus] >KAE8653262.1 hypothetical protein Csa_019821 [Cucumis sativus])

HSP 1 Score: 563.5 bits (1451), Expect = 1.6e-156
Identity = 302/374 (80.75%), Postives = 326/374 (87.17%), Query Frame = 0

Query: 42  EEEMPILRTEHWHEVVKTPISAKKRNISIP--NSPDVKCCSSSCAFSRLEHSNLESLNRS 101
           EEE+ IL+TEH  E +  PI AKK N  IP   S +VKCCSS CAFSRLEHSNLESL RS
Sbjct: 3   EEEVLILKTEHSDE-INIPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKRS 62

Query: 102 KSTMKLGGLIFFYAIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGWDA 161
           KS MKLGGLI FY IAIVVEIIGGLRANSL+VMTDAAHLLSDVAGFSVSLFAVWVSGW+A
Sbjct: 63  KSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEA 122

Query: 162 TPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAVDRILGQKTKVNGFIMFAIAAFGFIL 221
           TPQHSFGYNRLEVLGALVSVQLIWLISG+LIYEA+DRIL  KTKV+GF+MFA+AAFGF+L
Sbjct: 123 TPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFLL 182

Query: 222 NLFMVMWLGHSHSHSHGHSHSHSCHHSH--HSHQNHLDHDTDHEKEEIYTMTKQEGVPLG 281
           NLFMV+WLGHSH H H HS SH CHH H  HSHQNHL    +HE+EE+YT+TKQEG  LG
Sbjct: 183 NLFMVIWLGHSHHHHHSHS-SHCCHHDHHSHSHQNHL----EHEEEEVYTLTKQEGASLG 242

Query: 282 PKDNGSMLNINLQGAYLHVITDMIQSIGVMIAGAVLWVKPEWTVVDLICTLVFSVLAMAT 341
            KDN S LNINLQGAYLHVITDMIQSIGVMIAG VLW KPEW VVDLICTLVFSVLA+AT
Sbjct: 243 SKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALAT 302

Query: 342 TFSMLRHTAGILMEGTPREVNIESLENDLKGIKGVLALHDLHIWSITVGKVVLSCHVVAE 401
           TFSMLRHTA ILMEGTPREV+IESLEND+K +KGV  LHDLHIWSITVGKVVLSCHVVAE
Sbjct: 303 TFSMLRHTAVILMEGTPREVHIESLENDIKNMKGVYDLHDLHIWSITVGKVVLSCHVVAE 362

Query: 402 HGVPAREIISKIKN 412
            GV +RE+I KIK+
Sbjct: 363 AGVCSRELILKIKS 370

BLAST of Spg005773 vs. ExPASy Swiss-Prot
Match: Q6DBM8 (Metal tolerance protein B OS=Arabidopsis thaliana OX=3702 GN=MTPB PE=2 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 5.0e-97
Identity = 191/375 (50.93%), Postives = 258/375 (68.80%), Query Frame = 0

Query: 43  EEMPILRTEHWHEVVKTPISAKKRNISIPNSPDVKCCSSSCAFSRLEHSNLESLNRSKST 102
           E++ IL+ +   E+     S  + N+ +           SCAF+R EH   E+  R +ST
Sbjct: 4   EQICILKPDDEEEMESPSPSKTEENLGV--------VPLSCAFTRQEHCVSETKEREEST 63

Query: 103 MKLGGLIFFYAIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGWDATPQ 162
            +L  LIF Y I + V+I+GG +ANSLAVMTDAAHLLSDVAG  VSL A+ VS W+A P+
Sbjct: 64  RRLSSLIFLYLIVMSVQIVGGFKANSLAVMTDAAHLLSDVAGLCVSLLAIKVSSWEANPR 123

Query: 163 HSFGYNRLEVLGALVSVQLIWLISGVLIYEAVDRILGQKTKVNGFIMFAIAAFGFILNLF 222
           +SFG+ RLEVL A +SVQLIWL+SGV+I+EA+ R+L +  +VNG IMF I+AFGF +NL 
Sbjct: 124 NSFGFKRLEVLAAFLSVQLIWLVSGVIIHEAIQRLLSRSREVNGEIMFGISAFGFFMNLV 183

Query: 223 MVMWLGHSHSHSHGHSHSHSCHHSHHSHQNHLDHDTDHEKEEIYTMTKQEGVPL-GPKDN 282
           MV+WLGH+HSH     H H  HH HH+H++   H   H KE +    ++E  PL G K +
Sbjct: 184 MVLWLGHNHSH-----HHHDHHHHHHNHKHQHQH---HHKEVVAEEEEEEMNPLKGEKSS 243

Query: 283 GSMLNINLQGAYLHVITDMIQSIGVMIAGAVLWVKPEWTVVDLICTLVFSVLAMATTFSM 342
              +NIN+QGAYLH + DMIQS+GVMI G ++WVKP+W +VDLICTLVFS  A+A T  +
Sbjct: 244 SKEMNINIQGAYLHAMADMIQSLGVMIGGGIIWVKPKWVLVDLICTLVFSAFALAATLPI 303

Query: 343 LRHTAGILMEGTPREVNIESLENDLKGIKGVLALHDLHIWSITVGKVVLSCHVVAEHGVP 402
           L++  GILME  PR+++IE LE  LK I GV  ++DLH+W ITVG++VLSCH++ E G  
Sbjct: 304 LKNIFGILMERVPRDMDIEKLERGLKRIDGVKIVYDLHVWEITVGRIVLSCHILPEPGAS 362

Query: 403 AREIISKIKNLSLKN 417
            +EII+ ++N   K+
Sbjct: 364 PKEIITGVRNFCRKS 362

BLAST of Spg005773 vs. ExPASy Swiss-Prot
Match: Q9ZT63 (Metal tolerance protein 1 OS=Arabidopsis thaliana OX=3702 GN=MTP1 PE=1 SV=2)

HSP 1 Score: 313.5 bits (802), Expect = 3.7e-84
Identity = 159/350 (45.43%), Postives = 237/350 (67.71%), Query Frame = 0

Query: 78  CCSSSCAFSRLEHSNLESLNRSKSTMKLGGLIFFYAIAIVVEIIGGLRANSLAVMTDAAH 137
           C  + C FS  ++++ ++  RS S  KL   +    + + VE++GG++ANSLA++TDAAH
Sbjct: 31  CGEAPCGFSDSKNASGDAHERSASMRKLCIAVVLCLVFMSVEVVGGIKANSLAILTDAAH 90

Query: 138 LLSDVAGFSVSLFAVWVSGWDATPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAVDRI 197
           LLSDVA F++SLF++W +GW+ATP+ ++G+ R+E+LGALVS+QLIWL++G+L+YEA+ RI
Sbjct: 91  LLSDVAAFAISLFSLWAAGWEATPRQTYGFFRIEILGALVSIQLIWLLTGILVYEAIIRI 150

Query: 198 LGQKTKVNGFIMFAIAAFGFILNLFMVMWLGHSHSHSHGHSHSH-------------SCH 257
           + + ++VNGF+MF +AAFG ++N+ M + LGH H HSHGH H H             + H
Sbjct: 151 VTETSEVNGFLMFLVAAFGLVVNIIMAVLLGHDHGHSHGHGHGHGHDHHNHSHGVTVTTH 210

Query: 258 HSHHSHQNHLDHDTDHEKEEIY-----TMTKQEGVPLGPKDNGSMLNINLQGAYLHVITD 317
           H HH H++   H    +K   +      +  +    +  K+     NINLQGAYLHV+ D
Sbjct: 211 HHHHDHEHGHSHGHGEDKHHAHGDVTEQLLDKSKTQVAAKEKRKR-NINLQGAYLHVLGD 270

Query: 318 MIQSIGVMIAGAVLWVKPEWTVVDLICTLVFSVLAMATTFSMLRHTAGILMEGTPREVNI 377
            IQS+GVMI GA++W  PEW +VDLICTL FSV+ + TT +M+R+   +LME TPRE++ 
Sbjct: 271 SIQSVGVMIGGAIIWYNPEWKIVDLICTLAFSVIVLGTTINMIRNILEVLMESTPREIDA 330

Query: 378 ESLENDLKGIKGVLALHDLHIWSITVGKVVLSCHVVAEHGVPAREIISKI 410
             LE  L  ++ V+A+H+LHIW+ITVGKV+L+CHV       A  +++K+
Sbjct: 331 TKLEKGLLEMEEVVAVHELHIWAITVGKVLLACHVNIRPEADADMVLNKV 379

BLAST of Spg005773 vs. ExPASy Swiss-Prot
Match: Q688R1 (Metal tolerance protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP1 PE=2 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 4.9e-84
Identity = 165/368 (44.84%), Postives = 234/368 (63.59%), Query Frame = 0

Query: 78  CCSSSCAFSRLEHSNLESLNRSKSTMKLGGLIFFYAIAIVVEIIGGLRANSLAVMTDAAH 137
           C  ++C FS   +S+ ++  R  S  KL   +    I + VE++GG++ANSLA++TDAAH
Sbjct: 31  CRGAACDFSDSSNSSKDARERMASMRKLIIAVILCIIFMAVEVVGGIKANSLAILTDAAH 90

Query: 138 LLSDVAGFSVSLFAVWVSGWDATPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAVDRI 197
           LLSDVA F++SLF++W +GW+ATPQ S+G+ R+E+LGALVS+QLIWL++G+L+YEA+ R+
Sbjct: 91  LLSDVAAFAISLFSLWAAGWEATPQQSYGFFRIEILGALVSIQLIWLLAGILVYEAIVRL 150

Query: 198 LGQKTKVNGFIMFAIAAFGFILNLFMVMWLGHSHSHSHGHSHSHSCHHSH------HSHQ 257
           + +  +V G +MFA++AFG  +N+ M + LGH H H HGH H H   H H      H H 
Sbjct: 151 INESGEVQGSLMFAVSAFGLFVNIIMAVLLGHDHGHGHGHGHGHGHSHDHDHGGSDHDHH 210

Query: 258 NHLD--------HDTDHEKEEIYTMTKQEGV---------PLGPKDNG------------ 317
           +H D        H+  H       +    G          PL   D G            
Sbjct: 211 HHEDQEHGHVHHHEDGHGNSITVNLHHHPGTGHHHHDAEEPLLKSDAGCDSTQSGAKDAK 270

Query: 318 -SMLNINLQGAYLHVITDMIQSIGVMIAGAVLWVKPEWTVVDLICTLVFSVLAMATTFSM 377
            +  NIN+  AYLHV+ D IQSIGVMI GA++W KPEW ++DLICTL+FSV+ + TT  M
Sbjct: 271 KARRNINVHSAYLHVLGDSIQSIGVMIGGAIIWYKPEWKIIDLICTLIFSVIVLFTTIKM 330

Query: 378 LRHTAGILMEGTPREVNIESLENDLKGIKGVLALHDLHIWSITVGKVVLSCHVVAEHGVP 410
           LR+   +LME TPRE++  SLEN L+ + GV+A+H+LHIW+ITVGKV+L+CHV       
Sbjct: 331 LRNILEVLMESTPREIDATSLENGLRDMDGVVAVHELHIWAITVGKVLLACHVTITQDAD 390

BLAST of Spg005773 vs. ExPASy Swiss-Prot
Match: Q9LXS1 (Metal tolerance protein A2 OS=Arabidopsis thaliana OX=3702 GN=MTPA2 PE=2 SV=2)

HSP 1 Score: 308.1 bits (788), Expect = 1.6e-82
Identity = 161/338 (47.63%), Postives = 234/338 (69.23%), Query Frame = 0

Query: 78  CCSSSCAFSRLEHSNLESLNRSKSTMKLGGLIFFYAIAIVVEIIGGLRANSLAVMTDAAH 137
           C  + C FS  + S++E+  R+ S  KL   +   AI IVVE++GG++ANSLA++TDAAH
Sbjct: 47  CGEAPCGFSDAKTSSIEAQERAASMRKLLIAVLLCAIFIVVEVVGGIKANSLAILTDAAH 106

Query: 138 LLSDVAGFSVSLFAVWVSGWDATPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAVDRI 197
           LLSDVA F++SLF++W SGW A PQ S+G+ R+E+LGALVS+Q+IWL++G+L+YEA+ R+
Sbjct: 107 LLSDVAAFAISLFSLWASGWKANPQQSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRL 166

Query: 198 LGQKTKVNGFIMFAIAAFGFILNLFMVMWLGHSHSHSHGHSHSHSCHHSH-HSH-----Q 257
                +V G +MFA++A G ++N+ M + LGH H H HGHSH +   HSH H H     +
Sbjct: 167 NNGSGEVEGSLMFAVSAVGLLVNIAMAILLGHDHGHGHGHSHDNGHGHSHDHGHGIAATE 226

Query: 258 NHLDHDTDHEKEEIYTMTKQEGVPLGPKDNGSMLNINLQGAYLHVITDMIQSIGVMIAGA 317
           +H  HD+ H++ ++  +  ++             N+N+QGAYLHV+ D IQS+GVMI GA
Sbjct: 227 HH--HDSGHDESQLSDVLIEQ---------KKQRNVNIQGAYLHVLGDSIQSVGVMIGGA 286

Query: 318 VLWVKPEWTVVDLICTLVFSVLAMATTFSMLRHTAGILMEGTPREVNIESLENDLKGIKG 377
           ++W KPEW ++DLICTLVFSV+ + TT  MLR+   +LME TPRE++   LE  +  I+ 
Sbjct: 287 IIWYKPEWKILDLICTLVFSVIVLGTTIGMLRNILEVLMESTPREIDPTMLEKGVCEIEE 346

Query: 378 VLALHDLHIWSITVGKVVLSCHVVAEHGVPAREIISKI 410
           V+A+H+LHIW+ITVGK++L+CHV       A  ++ KI
Sbjct: 347 VVAVHELHIWAITVGKLLLACHVKIRPEAEADMVLDKI 373

BLAST of Spg005773 vs. ExPASy Swiss-Prot
Match: Q9M271 (Metal tolerance protein A1 OS=Arabidopsis thaliana OX=3702 GN=MTPA1 PE=2 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 2.1e-71
Identity = 141/333 (42.34%), Postives = 213/333 (63.96%), Query Frame = 0

Query: 78  CCSSSCAFSRLEHSNLESLNRSKSTMKLGGLIFFYAIAIVVEIIGGLRANSLAVMTDAAH 137
           C  ++C FS    S+ ++  R+ S  KL  ++    + + +E++ G++ANSLA++ DAAH
Sbjct: 9   CGETACGFST---SSSDAKKRAASMRKLCFVVVLCLLFMSIEVVCGIKANSLAILADAAH 68

Query: 138 LLSDVAGFSVSLFAVWVSGWDATPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAVDRI 197
           LL+DV  F++S+ ++W S W+A P+ S+G+ R+E+LG LVS+QLIWL++G+L+YEAV R+
Sbjct: 69  LLTDVGAFAISMLSLWASSWEANPRQSYGFFRIEILGTLVSIQLIWLLTGILVYEAVTRL 128

Query: 198 LGQ-KTKVNGFIMFAIAAFGFILNLFMVMWLGHSHSHSHGHSHSHSCHHSHHSHQNHLDH 257
           + +    V+GF M  +AAFG ++N+ M++ LGH H H H H HSH   HS+         
Sbjct: 129 VQETNDDVDGFFMVLVAAFGLVVNIIMIVVLGHDHGHGHDHGHSHDHGHSY--------- 188

Query: 258 DTDHEKEEIYTMTKQEGVPLGPKDNGSMLNINLQGAYLHVITDMIQSIGVMIAGAVLWVK 317
                 E++   +K+            + NIN+QGAYLHV+ D+IQSIGVMI G ++W  
Sbjct: 189 --GERAEQLLEKSKE------------IRNINVQGAYLHVLGDLIQSIGVMIGGGMIWYN 248

Query: 318 PEWTVVDLICTLVFSVLAMATTFSMLRHTAGILMEGTPREVNIESLENDLKGIKGVLALH 377
           P+W V+DLICTL FSV+ + TT  MLR    +LME TPRE++   LE  L  I+ V+ +H
Sbjct: 249 PKWKVIDLICTLFFSVIVLGTTIKMLRSILEVLMESTPREIDARQLEKGLMEIEEVVDVH 308

Query: 378 DLHIWSITVGKVVLSCHVVAEHGVPAREIISKI 410
           +LHIW+ITVGK + SCHV          +++K+
Sbjct: 309 ELHIWAITVGKALFSCHVKVRPEAGDEMVLNKV 315

BLAST of Spg005773 vs. ExPASy TrEMBL
Match: A0A1S3C537 (metal tolerance protein B OS=Cucumis melo OX=3656 GN=LOC103497126 PE=4 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 2.2e-164
Identity = 310/374 (82.89%), Postives = 332/374 (88.77%), Query Frame = 0

Query: 42  EEEMPILRTEHWHEVVKTPISAKKRNISIPNSPDVKCCSSSCAFSRLEHSNLESLNRSKS 101
           EEE+ IL+TEHW E VK PI AKKRN+ IP S +VKCC+S CAFSRLEHSNLESL RSKS
Sbjct: 3   EEEVLILKTEHWDE-VKMPIVAKKRNVVIPTSSEVKCCNSGCAFSRLEHSNLESLKRSKS 62

Query: 102 TMKLGGLIFFYAIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGWDATP 161
            MKLGG+I FY IAIVVEIIGGLRANSL+VMTDAAHLLSDVAGFSVSLFAVWVSGW+ATP
Sbjct: 63  AMKLGGMILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATP 122

Query: 162 QHSFGYNRLEVLGALVSVQLIWLISGVLIYEAVDRILGQKTKVNGFIMFAIAAFGFILNL 221
           QHSFGYNRLEVLGALVSVQLIWLISGVLIYEA+DRIL QKTKV+GF+MFA+AAFGFILNL
Sbjct: 123 QHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAVAAFGFILNL 182

Query: 222 FMVMWLGHSHSHSHGHSHSHSCHHSHHSH----QNHLDHDTDHEKEEIYTMTKQEGVPLG 281
           FMV+WLGHSH H H HS SH CHH HHSH    QNHL H++DHEKEEIYT+TK EG  LG
Sbjct: 183 FMVIWLGHSHHHHHSHS-SHCCHHDHHSHSHSDQNHLGHESDHEKEEIYTLTKLEGASLG 242

Query: 282 PKDNGSMLNINLQGAYLHVITDMIQSIGVMIAGAVLWVKPEWTVVDLICTLVFSVLAMAT 341
            KDN S LNINLQGAYLHVITDMIQSIGVMIAG VLW KPEW VVDLICTLVFSVLA+AT
Sbjct: 243 SKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGFVLWFKPEWIVVDLICTLVFSVLALAT 302

Query: 342 TFSMLRHTAGILMEGTPREVNIESLENDLKGIKGVLALHDLHIWSITVGKVVLSCHVVAE 401
           TFSMLRH A ILMEGTPREV+IESLEND+K IKGV  LHDLHIWSITVGKVVLSCHVVAE
Sbjct: 303 TFSMLRHIAVILMEGTPREVHIESLENDIKNIKGVHDLHDLHIWSITVGKVVLSCHVVAE 362

Query: 402 HGVPAREIISKIKN 412
            GV +RE+I KIK+
Sbjct: 363 AGVCSRELILKIKS 374

BLAST of Spg005773 vs. ExPASy TrEMBL
Match: A0A5D3BGQ9 (Metal tolerance protein B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001790 PE=4 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 2.2e-164
Identity = 310/374 (82.89%), Postives = 332/374 (88.77%), Query Frame = 0

Query: 42  EEEMPILRTEHWHEVVKTPISAKKRNISIPNSPDVKCCSSSCAFSRLEHSNLESLNRSKS 101
           EEE+ IL+TEHW E VK PI AKKRN+ IP S +VKCC+S CAFSRLEHSNLESL RSKS
Sbjct: 3   EEEVLILKTEHWDE-VKMPIVAKKRNVVIPTSSEVKCCNSGCAFSRLEHSNLESLKRSKS 62

Query: 102 TMKLGGLIFFYAIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGWDATP 161
            MKLGG+I FY IAIVVEIIGGLRANSL+VMTDAAHLLSDVAGFSVSLFAVWVSGW+ATP
Sbjct: 63  AMKLGGMILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEATP 122

Query: 162 QHSFGYNRLEVLGALVSVQLIWLISGVLIYEAVDRILGQKTKVNGFIMFAIAAFGFILNL 221
           QHSFGYNRLEVLGALVSVQLIWLISGVLIYEA+DRIL QKTKV+GF+MFA+AAFGFILNL
Sbjct: 123 QHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAVAAFGFILNL 182

Query: 222 FMVMWLGHSHSHSHGHSHSHSCHHSHHSH----QNHLDHDTDHEKEEIYTMTKQEGVPLG 281
           FMV+WLGHSH H H HS SH CHH HHSH    QNHL H++DHEKEEIYT+TK EG  LG
Sbjct: 183 FMVIWLGHSHHHHHSHS-SHCCHHDHHSHSHSDQNHLGHESDHEKEEIYTLTKLEGASLG 242

Query: 282 PKDNGSMLNINLQGAYLHVITDMIQSIGVMIAGAVLWVKPEWTVVDLICTLVFSVLAMAT 341
            KDN S LNINLQGAYLHVITDMIQSIGVMIAG VLW KPEW VVDLICTLVFSVLA+AT
Sbjct: 243 SKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGFVLWFKPEWIVVDLICTLVFSVLALAT 302

Query: 342 TFSMLRHTAGILMEGTPREVNIESLENDLKGIKGVLALHDLHIWSITVGKVVLSCHVVAE 401
           TFSMLRH A ILMEGTPREV+IESLEND+K IKGV  LHDLHIWSITVGKVVLSCHVVAE
Sbjct: 303 TFSMLRHIAVILMEGTPREVHIESLENDIKNIKGVHDLHDLHIWSITVGKVVLSCHVVAE 362

Query: 402 HGVPAREIISKIKN 412
            GV +RE+I KIK+
Sbjct: 363 AGVCSRELILKIKS 374

BLAST of Spg005773 vs. ExPASy TrEMBL
Match: A0A5A7SNE8 (Metal tolerance protein B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold848G00010 PE=4 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 4.2e-163
Identity = 307/374 (82.09%), Postives = 330/374 (88.24%), Query Frame = 0

Query: 42  EEEMPILRTEHWHEVVKTPISAKKRNISIPNSPDVKCCSSSCAFSRLEHSNLESLNRSKS 101
           EEE+ IL+TEHW E VK PI AKKRN+ IP S +VKCC+S CAFSRLEHSNLESL RSKS
Sbjct: 3   EEEVLILKTEHWDE-VKMPIVAKKRNVVIPTSSEVKCCNSGCAFSRLEHSNLESLKRSKS 62

Query: 102 TMKLGGLIFFYAIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGWDATP 161
            MKLGG+I FY IAIVVEIIGG RANSL+VMTDAAHLLSDVAGFSVSLFAVWVSGW+ TP
Sbjct: 63  AMKLGGMILFYTIAIVVEIIGGFRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEVTP 122

Query: 162 QHSFGYNRLEVLGALVSVQLIWLISGVLIYEAVDRILGQKTKVNGFIMFAIAAFGFILNL 221
           QHSFGYNRLEVLGALVSVQLIWLISGVLIYEA+DRIL QKTKV+GF+MFA+AAFGFILNL
Sbjct: 123 QHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAVAAFGFILNL 182

Query: 222 FMVMWLGHSHSHSHGHSHSHSCHHSHHSH----QNHLDHDTDHEKEEIYTMTKQEGVPLG 281
           FMV+WLGHSH H H HS SH CHH HHSH    QNHL H++DHEKEEIYT+TK EG  LG
Sbjct: 183 FMVIWLGHSHHHHHSHS-SHCCHHDHHSHSHSDQNHLGHESDHEKEEIYTLTKLEGASLG 242

Query: 282 PKDNGSMLNINLQGAYLHVITDMIQSIGVMIAGAVLWVKPEWTVVDLICTLVFSVLAMAT 341
            KDN S LNINLQGAYLHVITD+IQSIGVMIAG VLW KPEW VVDLICTLVFSVLA+AT
Sbjct: 243 SKDNSSTLNINLQGAYLHVITDLIQSIGVMIAGFVLWFKPEWIVVDLICTLVFSVLALAT 302

Query: 342 TFSMLRHTAGILMEGTPREVNIESLENDLKGIKGVLALHDLHIWSITVGKVVLSCHVVAE 401
           TFSMLRH A ILMEGTPREV+IESLEND+K IKGV  LHDLHIWSITVGKVVLSCHVVAE
Sbjct: 303 TFSMLRHIAVILMEGTPREVHIESLENDIKNIKGVHDLHDLHIWSITVGKVVLSCHVVAE 362

Query: 402 HGVPAREIISKIKN 412
            GV +RE+I KIK+
Sbjct: 363 AGVCSRELILKIKS 374

BLAST of Spg005773 vs. ExPASy TrEMBL
Match: I1ZI47 (Metal transport protein 4 OS=Cucumis sativus OX=3659 GN=MTP4 PE=2 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 7.7e-157
Identity = 302/374 (80.75%), Postives = 326/374 (87.17%), Query Frame = 0

Query: 42  EEEMPILRTEHWHEVVKTPISAKKRNISIP--NSPDVKCCSSSCAFSRLEHSNLESLNRS 101
           EEE+ IL+TEH  E +  PI AKK N  IP   S +VKCCSS CAFSRLEHSNLESL RS
Sbjct: 3   EEEVLILKTEHSDE-INIPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKRS 62

Query: 102 KSTMKLGGLIFFYAIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGWDA 161
           KS MKLGGLI FY IAIVVEIIGGLRANSL+VMTDAAHLLSDVAGFSVSLFAVWVSGW+A
Sbjct: 63  KSAMKLGGLILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEA 122

Query: 162 TPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAVDRILGQKTKVNGFIMFAIAAFGFIL 221
           TPQHSFGYNRLEVLGALVSVQLIWLISG+LIYEA+DRIL  KTKV+GF+MFA+AAFGF+L
Sbjct: 123 TPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFLL 182

Query: 222 NLFMVMWLGHSHSHSHGHSHSHSCHHSH--HSHQNHLDHDTDHEKEEIYTMTKQEGVPLG 281
           NLFMV+WLGHSH H H HS SH CHH H  HSHQNHL    +HE+EE+YT+TKQEG  LG
Sbjct: 183 NLFMVIWLGHSHHHHHSHS-SHCCHHDHHSHSHQNHL----EHEEEEVYTLTKQEGASLG 242

Query: 282 PKDNGSMLNINLQGAYLHVITDMIQSIGVMIAGAVLWVKPEWTVVDLICTLVFSVLAMAT 341
            KDN S LNINLQGAYLHVITDMIQSIGVMIAG VLW KPEW VVDLICTLVFSVLA+AT
Sbjct: 243 SKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALAT 302

Query: 342 TFSMLRHTAGILMEGTPREVNIESLENDLKGIKGVLALHDLHIWSITVGKVVLSCHVVAE 401
           TFSMLRHTA ILMEGTPREV+IESLEND+K +KGV  LHDLHIWSITVGKVVLSCHVVAE
Sbjct: 303 TFSMLRHTAVILMEGTPREVHIESLENDIKNMKGVYDLHDLHIWSITVGKVVLSCHVVAE 362

Query: 402 HGVPAREIISKIKN 412
            GV +RE+I KIK+
Sbjct: 363 AGVCSRELILKIKS 370

BLAST of Spg005773 vs. ExPASy TrEMBL
Match: A0A0A0M0L3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G525270 PE=4 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 2.2e-156
Identity = 301/374 (80.48%), Postives = 325/374 (86.90%), Query Frame = 0

Query: 42  EEEMPILRTEHWHEVVKTPISAKKRNISIP--NSPDVKCCSSSCAFSRLEHSNLESLNRS 101
           EEE+ IL+TEH  E +  PI AKK N  IP   S +VKCCSS CAFSRLEHSNLESL RS
Sbjct: 3   EEEVLILKTEHSDE-INIPIVAKKMNDVIPTSTSSEVKCCSSGCAFSRLEHSNLESLKRS 62

Query: 102 KSTMKLGGLIFFYAIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGWDA 161
           KS MKLGGLI FY IAIVVEIIGG RANSL+VMTDAAHLLSDVAGFSVSLFAVWVSGW+A
Sbjct: 63  KSAMKLGGLILFYTIAIVVEIIGGFRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEA 122

Query: 162 TPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAVDRILGQKTKVNGFIMFAIAAFGFIL 221
           TPQHSFGYNRLEVLGALVSVQLIWLISG+LIYEA+DRIL  KTKV+GF+MFA+AAFGF+L
Sbjct: 123 TPQHSFGYNRLEVLGALVSVQLIWLISGILIYEAIDRILAPKTKVDGFLMFAVAAFGFLL 182

Query: 222 NLFMVMWLGHSHSHSHGHSHSHSCHHSH--HSHQNHLDHDTDHEKEEIYTMTKQEGVPLG 281
           NLFMV+WLGHSH H H HS SH CHH H  HSHQNHL    +HE+EE+YT+TKQEG  LG
Sbjct: 183 NLFMVIWLGHSHHHHHSHS-SHCCHHDHHSHSHQNHL----EHEEEEVYTLTKQEGASLG 242

Query: 282 PKDNGSMLNINLQGAYLHVITDMIQSIGVMIAGAVLWVKPEWTVVDLICTLVFSVLAMAT 341
            KDN S LNINLQGAYLHVITDMIQSIGVMIAG VLW KPEW VVDLICTLVFSVLA+AT
Sbjct: 243 SKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGLVLWFKPEWIVVDLICTLVFSVLALAT 302

Query: 342 TFSMLRHTAGILMEGTPREVNIESLENDLKGIKGVLALHDLHIWSITVGKVVLSCHVVAE 401
           TFSMLRHTA ILMEGTPREV+IESLEND+K +KGV  LHDLHIWSITVGKVVLSCHVVAE
Sbjct: 303 TFSMLRHTAVILMEGTPREVHIESLENDIKNMKGVYDLHDLHIWSITVGKVVLSCHVVAE 362

Query: 402 HGVPAREIISKIKN 412
            GV +RE+I KIK+
Sbjct: 363 AGVCSRELILKIKS 370

BLAST of Spg005773 vs. TAIR 10
Match: AT2G29410.1 (metal tolerance protein B1 )

HSP 1 Score: 356.3 bits (913), Expect = 3.6e-98
Identity = 191/375 (50.93%), Postives = 258/375 (68.80%), Query Frame = 0

Query: 43  EEMPILRTEHWHEVVKTPISAKKRNISIPNSPDVKCCSSSCAFSRLEHSNLESLNRSKST 102
           E++ IL+ +   E+     S  + N+ +           SCAF+R EH   E+  R +ST
Sbjct: 4   EQICILKPDDEEEMESPSPSKTEENLGV--------VPLSCAFTRQEHCVSETKEREEST 63

Query: 103 MKLGGLIFFYAIAIVVEIIGGLRANSLAVMTDAAHLLSDVAGFSVSLFAVWVSGWDATPQ 162
            +L  LIF Y I + V+I+GG +ANSLAVMTDAAHLLSDVAG  VSL A+ VS W+A P+
Sbjct: 64  RRLSSLIFLYLIVMSVQIVGGFKANSLAVMTDAAHLLSDVAGLCVSLLAIKVSSWEANPR 123

Query: 163 HSFGYNRLEVLGALVSVQLIWLISGVLIYEAVDRILGQKTKVNGFIMFAIAAFGFILNLF 222
           +SFG+ RLEVL A +SVQLIWL+SGV+I+EA+ R+L +  +VNG IMF I+AFGF +NL 
Sbjct: 124 NSFGFKRLEVLAAFLSVQLIWLVSGVIIHEAIQRLLSRSREVNGEIMFGISAFGFFMNLV 183

Query: 223 MVMWLGHSHSHSHGHSHSHSCHHSHHSHQNHLDHDTDHEKEEIYTMTKQEGVPL-GPKDN 282
           MV+WLGH+HSH     H H  HH HH+H++   H   H KE +    ++E  PL G K +
Sbjct: 184 MVLWLGHNHSH-----HHHDHHHHHHNHKHQHQH---HHKEVVAEEEEEEMNPLKGEKSS 243

Query: 283 GSMLNINLQGAYLHVITDMIQSIGVMIAGAVLWVKPEWTVVDLICTLVFSVLAMATTFSM 342
              +NIN+QGAYLH + DMIQS+GVMI G ++WVKP+W +VDLICTLVFS  A+A T  +
Sbjct: 244 SKEMNINIQGAYLHAMADMIQSLGVMIGGGIIWVKPKWVLVDLICTLVFSAFALAATLPI 303

Query: 343 LRHTAGILMEGTPREVNIESLENDLKGIKGVLALHDLHIWSITVGKVVLSCHVVAEHGVP 402
           L++  GILME  PR+++IE LE  LK I GV  ++DLH+W ITVG++VLSCH++ E G  
Sbjct: 304 LKNIFGILMERVPRDMDIEKLERGLKRIDGVKIVYDLHVWEITVGRIVLSCHILPEPGAS 362

Query: 403 AREIISKIKNLSLKN 417
            +EII+ ++N   K+
Sbjct: 364 PKEIITGVRNFCRKS 362

BLAST of Spg005773 vs. TAIR 10
Match: AT2G46800.1 (zinc transporter of Arabidopsis thaliana )

HSP 1 Score: 313.5 bits (802), Expect = 2.7e-85
Identity = 159/350 (45.43%), Postives = 237/350 (67.71%), Query Frame = 0

Query: 78  CCSSSCAFSRLEHSNLESLNRSKSTMKLGGLIFFYAIAIVVEIIGGLRANSLAVMTDAAH 137
           C  + C FS  ++++ ++  RS S  KL   +    + + VE++GG++ANSLA++TDAAH
Sbjct: 31  CGEAPCGFSDSKNASGDAHERSASMRKLCIAVVLCLVFMSVEVVGGIKANSLAILTDAAH 90

Query: 138 LLSDVAGFSVSLFAVWVSGWDATPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAVDRI 197
           LLSDVA F++SLF++W +GW+ATP+ ++G+ R+E+LGALVS+QLIWL++G+L+YEA+ RI
Sbjct: 91  LLSDVAAFAISLFSLWAAGWEATPRQTYGFFRIEILGALVSIQLIWLLTGILVYEAIIRI 150

Query: 198 LGQKTKVNGFIMFAIAAFGFILNLFMVMWLGHSHSHSHGHSHSH-------------SCH 257
           + + ++VNGF+MF +AAFG ++N+ M + LGH H HSHGH H H             + H
Sbjct: 151 VTETSEVNGFLMFLVAAFGLVVNIIMAVLLGHDHGHSHGHGHGHGHDHHNHSHGVTVTTH 210

Query: 258 HSHHSHQNHLDHDTDHEKEEIY-----TMTKQEGVPLGPKDNGSMLNINLQGAYLHVITD 317
           H HH H++   H    +K   +      +  +    +  K+     NINLQGAYLHV+ D
Sbjct: 211 HHHHDHEHGHSHGHGEDKHHAHGDVTEQLLDKSKTQVAAKEKRKR-NINLQGAYLHVLGD 270

Query: 318 MIQSIGVMIAGAVLWVKPEWTVVDLICTLVFSVLAMATTFSMLRHTAGILMEGTPREVNI 377
            IQS+GVMI GA++W  PEW +VDLICTL FSV+ + TT +M+R+   +LME TPRE++ 
Sbjct: 271 SIQSVGVMIGGAIIWYNPEWKIVDLICTLAFSVIVLGTTINMIRNILEVLMESTPREIDA 330

Query: 378 ESLENDLKGIKGVLALHDLHIWSITVGKVVLSCHVVAEHGVPAREIISKI 410
             LE  L  ++ V+A+H+LHIW+ITVGKV+L+CHV       A  +++K+
Sbjct: 331 TKLEKGLLEMEEVVAVHELHIWAITVGKVLLACHVNIRPEADADMVLNKV 379

BLAST of Spg005773 vs. TAIR 10
Match: AT2G46800.2 (zinc transporter of Arabidopsis thaliana )

HSP 1 Score: 313.5 bits (802), Expect = 2.7e-85
Identity = 159/350 (45.43%), Postives = 237/350 (67.71%), Query Frame = 0

Query: 78  CCSSSCAFSRLEHSNLESLNRSKSTMKLGGLIFFYAIAIVVEIIGGLRANSLAVMTDAAH 137
           C  + C FS  ++++ ++  RS S  KL   +    + + VE++GG++ANSLA++TDAAH
Sbjct: 31  CGEAPCGFSDSKNASGDAHERSASMRKLCIAVVLCLVFMSVEVVGGIKANSLAILTDAAH 90

Query: 138 LLSDVAGFSVSLFAVWVSGWDATPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAVDRI 197
           LLSDVA F++SLF++W +GW+ATP+ ++G+ R+E+LGALVS+QLIWL++G+L+YEA+ RI
Sbjct: 91  LLSDVAAFAISLFSLWAAGWEATPRQTYGFFRIEILGALVSIQLIWLLTGILVYEAIIRI 150

Query: 198 LGQKTKVNGFIMFAIAAFGFILNLFMVMWLGHSHSHSHGHSHSH-------------SCH 257
           + + ++VNGF+MF +AAFG ++N+ M + LGH H HSHGH H H             + H
Sbjct: 151 VTETSEVNGFLMFLVAAFGLVVNIIMAVLLGHDHGHSHGHGHGHGHDHHNHSHGVTVTTH 210

Query: 258 HSHHSHQNHLDHDTDHEKEEIY-----TMTKQEGVPLGPKDNGSMLNINLQGAYLHVITD 317
           H HH H++   H    +K   +      +  +    +  K+     NINLQGAYLHV+ D
Sbjct: 211 HHHHDHEHGHSHGHGEDKHHAHGDVTEQLLDKSKTQVAAKEKRKR-NINLQGAYLHVLGD 270

Query: 318 MIQSIGVMIAGAVLWVKPEWTVVDLICTLVFSVLAMATTFSMLRHTAGILMEGTPREVNI 377
            IQS+GVMI GA++W  PEW +VDLICTL FSV+ + TT +M+R+   +LME TPRE++ 
Sbjct: 271 SIQSVGVMIGGAIIWYNPEWKIVDLICTLAFSVIVLGTTINMIRNILEVLMESTPREIDA 330

Query: 378 ESLENDLKGIKGVLALHDLHIWSITVGKVVLSCHVVAEHGVPAREIISKI 410
             LE  L  ++ V+A+H+LHIW+ITVGKV+L+CHV       A  +++K+
Sbjct: 331 TKLEKGLLEMEEVVAVHELHIWAITVGKVLLACHVNIRPEADADMVLNKV 379

BLAST of Spg005773 vs. TAIR 10
Match: AT3G58810.1 (metal tolerance protein A2 )

HSP 1 Score: 308.1 bits (788), Expect = 1.1e-83
Identity = 161/338 (47.63%), Postives = 234/338 (69.23%), Query Frame = 0

Query: 78  CCSSSCAFSRLEHSNLESLNRSKSTMKLGGLIFFYAIAIVVEIIGGLRANSLAVMTDAAH 137
           C  + C FS  + S++E+  R+ S  KL   +   AI IVVE++GG++ANSLA++TDAAH
Sbjct: 86  CGEAPCGFSDAKTSSIEAQERAASMRKLLIAVLLCAIFIVVEVVGGIKANSLAILTDAAH 145

Query: 138 LLSDVAGFSVSLFAVWVSGWDATPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAVDRI 197
           LLSDVA F++SLF++W SGW A PQ S+G+ R+E+LGALVS+Q+IWL++G+L+YEA+ R+
Sbjct: 146 LLSDVAAFAISLFSLWASGWKANPQQSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRL 205

Query: 198 LGQKTKVNGFIMFAIAAFGFILNLFMVMWLGHSHSHSHGHSHSHSCHHSH-HSH-----Q 257
                +V G +MFA++A G ++N+ M + LGH H H HGHSH +   HSH H H     +
Sbjct: 206 NNGSGEVEGSLMFAVSAVGLLVNIAMAILLGHDHGHGHGHSHDNGHGHSHDHGHGIAATE 265

Query: 258 NHLDHDTDHEKEEIYTMTKQEGVPLGPKDNGSMLNINLQGAYLHVITDMIQSIGVMIAGA 317
           +H  HD+ H++ ++  +  ++             N+N+QGAYLHV+ D IQS+GVMI GA
Sbjct: 266 HH--HDSGHDESQLSDVLIEQ---------KKQRNVNIQGAYLHVLGDSIQSVGVMIGGA 325

Query: 318 VLWVKPEWTVVDLICTLVFSVLAMATTFSMLRHTAGILMEGTPREVNIESLENDLKGIKG 377
           ++W KPEW ++DLICTLVFSV+ + TT  MLR+   +LME TPRE++   LE  +  I+ 
Sbjct: 326 IIWYKPEWKILDLICTLVFSVIVLGTTIGMLRNILEVLMESTPREIDPTMLEKGVCEIEE 385

Query: 378 VLALHDLHIWSITVGKVVLSCHVVAEHGVPAREIISKI 410
           V+A+H+LHIW+ITVGK++L+CHV       A  ++ KI
Sbjct: 386 VVAVHELHIWAITVGKLLLACHVKIRPEAEADMVLDKI 412

BLAST of Spg005773 vs. TAIR 10
Match: AT3G58810.2 (metal tolerance protein A2 )

HSP 1 Score: 308.1 bits (788), Expect = 1.1e-83
Identity = 161/338 (47.63%), Postives = 234/338 (69.23%), Query Frame = 0

Query: 78  CCSSSCAFSRLEHSNLESLNRSKSTMKLGGLIFFYAIAIVVEIIGGLRANSLAVMTDAAH 137
           C  + C FS  + S++E+  R+ S  KL   +   AI IVVE++GG++ANSLA++TDAAH
Sbjct: 47  CGEAPCGFSDAKTSSIEAQERAASMRKLLIAVLLCAIFIVVEVVGGIKANSLAILTDAAH 106

Query: 138 LLSDVAGFSVSLFAVWVSGWDATPQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAVDRI 197
           LLSDVA F++SLF++W SGW A PQ S+G+ R+E+LGALVS+Q+IWL++G+L+YEA+ R+
Sbjct: 107 LLSDVAAFAISLFSLWASGWKANPQQSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRL 166

Query: 198 LGQKTKVNGFIMFAIAAFGFILNLFMVMWLGHSHSHSHGHSHSHSCHHSH-HSH-----Q 257
                +V G +MFA++A G ++N+ M + LGH H H HGHSH +   HSH H H     +
Sbjct: 167 NNGSGEVEGSLMFAVSAVGLLVNIAMAILLGHDHGHGHGHSHDNGHGHSHDHGHGIAATE 226

Query: 258 NHLDHDTDHEKEEIYTMTKQEGVPLGPKDNGSMLNINLQGAYLHVITDMIQSIGVMIAGA 317
           +H  HD+ H++ ++  +  ++             N+N+QGAYLHV+ D IQS+GVMI GA
Sbjct: 227 HH--HDSGHDESQLSDVLIEQ---------KKQRNVNIQGAYLHVLGDSIQSVGVMIGGA 286

Query: 318 VLWVKPEWTVVDLICTLVFSVLAMATTFSMLRHTAGILMEGTPREVNIESLENDLKGIKG 377
           ++W KPEW ++DLICTLVFSV+ + TT  MLR+   +LME TPRE++   LE  +  I+ 
Sbjct: 287 IIWYKPEWKILDLICTLVFSVIVLGTTIGMLRNILEVLMESTPREIDPTMLEKGVCEIEE 346

Query: 378 VLALHDLHIWSITVGKVVLSCHVVAEHGVPAREIISKI 410
           V+A+H+LHIW+ITVGK++L+CHV       A  ++ KI
Sbjct: 347 VVAVHELHIWAITVGKLLLACHVKIRPEAEADMVLDKI 373