Spg005756 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg005756
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionProtein kinase APK1B
Locationscaffold11: 2042856 .. 2048153 (-)
RNA-Seq ExpressionSpg005756
SyntenySpg005756
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACCCATGTGGATTGCTTGAAGTAAGTGAAGAAAATTGTGGAAAAATGGGAATATGGGAGTTTGTTTGTTTCCTAAGAAAATGATAGAAAATTTGACCATAACAGTGTAAGACTCTGAATATGTAATAAAATGCCAAATTTGAACAAAAAGTTACACTTTGATCAAACCTAAATCCATCTGGAAATTCTTTGATCATTGGTTGGCCATCTTTTTCATTTAATTAAAACAAATAATAATAATAATGATAATATGTTACTTTTTAAAAAAATTTAATTTTTCCTTTTTACGGTTTAGTTACCGACCTTGTGGTTATCCGTACAACTTTATGTCAGTTGAACTATGCTCTTGTTGGTTTAGAATTTAAATCTACTACCAAATGGATATTAAAAAACACTGAAAACATGGATCACAACCATACTTTTCATTAAATCACTCGTTTTCGAATTCTTGTTTTTAGATCATCAATCAAGTGTACCTTAATCTTTTAAAATATTTTCATTTTTGGTTATAAAAACCATTCAAAAATGAAAATTTTATTTATAATTTTTCTACATATTAAATTTAAAATATAACAACTAAAATAAATTAATGAAATGCCAAAACTGCATTAAGATTATTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAAACCTCATTGCCAAACATGCTCTGAGTTGCTTTGTAATTAATAATAGAACCACTTTTCACTTTTCTATAATCACTCTGAAAACCTCATCGGCAAACATGCTTTGAGATCTTTTTTTATTATTAAACAAAACCACTTCTCGTTCTTCCTAAGCATTGGAAAAAACCAAAAAAATTGAGATTTTGTGGAGAGTGTGGGATTCAAACAGCAAACATCTTTGCCATTAGTACAAGTTTGATATCACTTGAGCTAGGTCCATGTTGCCAAAAAATTGACATACTTTTGATCCTATATTTTTAGGAGCATTCTTGAACTGTAGTGAGTAATGAATTAGTCTATGTATTTTTTAAATTATAACAATTTAATTGATAAACTTTACTCTATATCAATTAATTTATACGGTTTAACATTTATGACGATTTAGTTCTAAAATCTGTGAGGAAAACACTCTTTGGTCTCTAAGTTTTGATAATAACTTTTATTTGGTCTCTAGGTTTCAATATGTTACACTTTTAATTGTTGAGTTTTGAGTTTAGTTTCTATTTGGTCTCTAGATTTCAAAATTTTACACTTTTATGCCTTAATTTTCACTAATACTCACTTTTGGTTTTGGTTCGTTGTGATAATTTTCTATTATTCAATTTAAAAGAATTAAATTAATCATAATTAAACTAGTTTTCACTAATTTTCCATCACTATTGAAAGTATTTTAAACATTTTTGATCCTTGAAGTTTGGATAGGCTGCATTTTGATTTCTGAATTTTCAAACTTAACAAATATACCACTGAGTAAAATAATTTTTTAATAGAAAAAATAGTGTCATGTTAGCGGTAATTAACAGAAATTTGGACGTAAGGACCAATGGTGTAATGTGAGAGATTTGAATCACAGACCTATTGCTCATTAGTACAAACTTTATTGTAAGTTGAGCTATGCTCGTGTTTACTGATAAAACTAAATCATTATATACTAGAGTTTAAAGATCAAACCGTTTTATGAAAGCATAACTCAACTGACATTTGTGGTTCGAATCTCACATTTCACGAGTTGTGAAAAAGAAAAAAAAAAAAAAATCAAAGCGTTCCAAATTTAGAAGTAAAGAGACCAAAGGGAAAAAGAAAAACCGAAAACAGAACACACGGTTAATAGAAATTCGCGAAACCAGAGTGTTGCAGTGATCACTGATGAGTTCTCTTTGACTTTTAGTTGATTCCATGGATTCTCGACGTGGTGAAATCAAATGGTGGAGAAAATCGCATCACAATTCTGCAAACCGAAGCCTTATCTGCTTCTGCTTCTGCTTCTGATTTGACCAGGTAAAAGCAAATTGAAAAAAGGGTCCATCCAAAAGTTCTAGGGCAAAATGCGGGGCCAGGGACTGACCGCCATTAGTGCAAATTTCTCCACCTTCGCGCACTGGACCCCCTCCAACCATCTCCCACTGCTCCATTCTTTATTCATTTCATTCTACTCTCGCACAACCTTCCTCAATTTTCCCAGTCAAAGTCTCTCAACTCTTTCCCCTCCTTTCGTTCTCTCTAAATACCTCTTCAAAGATTCCTCCCCAATTCGATTCCACCCTTCTCAACTTCCTTTCTTGCTCAGCTCTGACTCTGTGGGCCGAACTGGTAATTCAATTTGCTCCCTCCCCTGTTCTGGGTTTTCGGGATTTTTTCTGTTTTCTCTCGATTTTGTTGTTTATACTTGAATTCTGAAGCTCTGGTTGCATGCAAGTGTTTATTGGGGTTTTTTACTCCTTTTGAGGGATACCCATTTCTCCGTGGTTGTGAATCTCCATTTTCGAACTCGGATCTGCAAGTTTTTGCTCTTCGGTTTGGTTTACTGTTGAAATTTCTTGCTGTAAGCCGCTGAATTTTGTGATCCGTAGGGAGAAAACTGGTCAGGCTGTGGGATTTTGGGCCAGTTCCATGGGGATTTGCTTTAGCGCCAGAATTAAAGCTGTTAGCACATGCAGTACTGGTACAGGTAGAGCTCTGAAGCCATAGAAATGAATATCTTGCCCATTGTGTTTGAGTTTAATAGTTCATTTCTTTGCCCTTCTCTCTGTTTCTCCTCCCTCGTTTGTTTATTGAGAAAAGAAAAGGAAAAAACATGTTTTCATGATTTTACATAACAAATGGTTGGAGTTTCATTCTCCAGGTTTTAGACATTTGATGTGTATAGTATGTCAGTTTGATGAGCTTTTTTGGCTCAAATGATTGGATCTGTGAGTCAACTCATCACAACAAAAATTGCCATGTTTCTAAATTTGGCTACAATGGAATTGAGTTGAAGGTTTACTTGTAAAGAAACGAAAAAAAAAAAATATGAGATATTGTTTAAATTGGAACATGAAACTTTTATCTGTGGAACGGTAGGCGTTTGTTCGTTTGTTTATTGTTTGGTTTTGGTTGGTGTGTTCACTCTGCTTTTATTTTTTTTATACGCTCTTTTCCACTTGGGTTGCGTTTCTTTACTGGACCCTCACCAGCCATATTCTCTTGTGTTGGGAATTGGGATATTCTTGTTCTTTTGAATCTTTCTGGTATTTGGATGTTCTTACATGAATCGAACTTGGGGATTCGAGTCATGCAGACACTCAAAATTGTAGATGCAATGCAACTTGATTTGACAATGAAAGTTTTAGTTCAGGTATGGATATCTTACATTCAAGTCATGCAGGAGGAAACTTAAACAGCCTTGGCAGAGATGCAATCGATCTCTCTAGTTCTAGCAGCAACAGCAAGTTCTCATCAACATCAATTGCTCGGAGTGAGGATGAGATCTTGCAATCCTCAAATCTGAAGAGTTTTAGTTTTTCGGAACTTAAACTGGCCACTAGGAATTTCCGGCCTGATAGTTTGTTGGGGGAAGGTGGGTTTGGTTCGGTTTATAAAGGTTGGATCGATGAGCATTCATTTTCTGCTGCCAAGCCAGGGTCTGGCACGGTTGTTGCAGTGAAAAGGCTAAATCTAGATGGTCTACAAGGCCACAAGGAGTGGCTGGTAAGTCAATGTTCTTTCCTTTAAAAAATGTCAGGCTGCCTATGACTTCTGCTTTATCTTTGACGTCAACAAAGTAAGCTATCAATCCACTTACCCTTTAGTTTCTTCAATTCTGTAGGCTGAAGTCAATTTTCTGGGACAACTTCATCATTCTCACCTAGTGAGACTGATAGGATATTGTTTGGAAGACGAACATCGGTTGCTGGTGTATGAATTCCTTCCCCGAGGCAGCTTGGAGAACCATTTGTTTAGGAGTGAGTTCTCATCTCCTCACTTATTAAGCAAAGGGGCTCCATTGTTTGGTAGTGTCAATTTATTTATATTATTGCAACTTTTTCTTTCAGGAGGGTTGTACTTTCAACCTCTTTCATGGAGCCTTCGCTTGAAGATCGCTCTTGGCGCTGCAAAGGGACTGGCTTTCCTTCACAGTGATGAAAACAAAGTGATCTACCGTGATTTCAAGACATCAAATATACTCCTTGATTCGGTAAGTAGTTGTTACGGTGTGGTGGGATTTCAGTTTTTGGCTGTTTTTCTGAAGTGTCCATTCTATGATATGGTTGCAGAACTACAATGCAAAGCTCTCTGATTTTGGCCTGGCCAAGGATGGACCTACGGGCGACAAAAGTCACGTCTCCACCAGAATTATGGGGACATATGGATATGCAGCTCCCGAGTATCTTGCTACAGGTAATATTCAGCTAATCGTTGCATCGATATACTTGTTCTAAAGAGGAAAAATAATCTTTTTCATGATCTCTTTTGTTAATCTTTTATTTCATCTGACTGTTAGGTCATCTTAGTGCCAAGAGTGACGTGTATAGTTTTGGGGTTGTTCTATTGGAGATGTTATCTGGTCGAAGAGCAATCGACAAGAACCGACCACCAGGGGAACAGAATCTGGTCGAGTGGGCCAAACCCTTCCTTGCTAATAAACGCAAGACTTTTCGTTTACTTGACGCACGTATTGAACGCAACTACTCCATGGAATCAGCTTTTAGAGTTGCAATCCTTGCATCACGTTGCCTATCTGCAGAACCCAAGTTCAGGCCGAGTATGAGCGAAATTGTAACAACGTTGATGGATCTCCAGGATTCCAAAGGAACTGGAGCTACTGGGAATAGAAGATCCAGGACTTGTAAGGAAAACACAGACAAGGGCAATGCACACAAGAAAGTCACTGCTTATCCTAGACCATCTGCCTCTGCTCTCTTTACATGAGTTCGGGTCTCTGCAAAAACATAACGAAAACCCGTCGAGGTGATCTGGGAGAGACGAGAGGGAAGAATCCCGGCTTCTACATCCTCGAGCGAATGTATAGTGTTCTTATTCCCACTAATGTATACTTGCATCACTACCTGGGAAACAAAAGTTGGTAGCATTGTAGCAAGAGTTGAAAATGCTACTGCTGTAGCGATGGATGTAGCTATTTTGTGTTTCAGTTTTGTAATGCTTTTTTCTTCTTGTTCTTTCAGAATTCTGTGATCTGTGTTATTGAGTTTGTCGTAAAATGCATAGTTTAATTAATGAAAGAGGATTTGAATCAGTTTAGAGATGATACTTGCTGTGGCTTGGATCATTGCCTTTTTACTTCTTAATTACATTTGCAAATGAGCGTGTGTGAAATTTAACATGAAATGCCG

mRNA sequence

ATGGACCCATGTGGATTGCTTGAATTGATTCCATGGATTCTCGACGTGGTGAAATCAAATGGTGGAGAAAATCGCATCACAATTCTGCAAACCGAAGCCTTATCTGCTTCTGCTTCTGCTTCTGATTTGACCAGGGAGAAAACTGGTCAGGCTGTGGGATTTTGGGCCAGTTCCATGGGGATTTGCTTTAGCGCCAGAATTAAAGCTGTTAGCACATGCAGTACTGGTACAGCCATATTCTCTTGTGTTGGGAATTGGGATATTCTTGTTCTTTTGAATCTTTCTGGTATGGATATCTTACATTCAAGTCATGCAGGAGGAAACTTAAACAGCCTTGGCAGAGATGCAATCGATCTCTCTAGTTCTAGCAGCAACAGCAAGTTCTCATCAACATCAATTGCTCGGAGTGAGGATGAGATCTTGCAATCCTCAAATCTGAAGAGTTTTAGTTTTTCGGAACTTAAACTGGCCACTAGGAATTTCCGGCCTGATAGTTTGTTGGGGGAAGGTGGGTTTGGTTCGGTTTATAAAGGTTGGATCGATGAGCATTCATTTTCTGCTGCCAAGCCAGGGTCTGGCACGGTTGTTGCAGTGAAAAGGCTAAATCTAGATGGTCTACAAGGCCACAAGGAGTGGCTGGCTGAAGTCAATTTTCTGGGACAACTTCATCATTCTCACCTAGTGAGACTGATAGGATATTGTTTGGAAGACGAACATCGGTTGCTGGTGTATGAATTCCTTCCCCGAGGCAGCTTGGAGAACCATTTGTTTAGGAGAGGGTTGTACTTTCAACCTCTTTCATGGAGCCTTCGCTTGAAGATCGCTCTTGGCGCTGCAAAGGGACTGGCTTTCCTTCACAGTGATGAAAACAAAGTGATCTACCGTGATTTCAAGACATCAAATATACTCCTTGATTCGAACTACAATGCAAAGCTCTCTGATTTTGGCCTGGCCAAGGATGGACCTACGGGCGACAAAAGTCACGTCTCCACCAGAATTATGGGGACATATGGATATGCAGCTCCCGAGTATCTTGCTACAGGTCATCTTAGTGCCAAGAGTGACGTGTATAGTTTTGGGGTTGTTCTATTGGAGATGTTATCTGGTCGAAGAGCAATCGACAAGAACCGACCACCAGGGGAACAGAATCTGGTCGAGTGGGCCAAACCCTTCCTTGCTAATAAACGCAAGACTTTTCGTTTACTTGACGCACGTATTGAACGCAACTACTCCATGGAATCAGCTTTTAGAGTTGCAATCCTTGCATCACGTTGCCTATCTGCAGAACCCAAGTTCAGGCCGAGTATGAGCGAAATTGTAACAACGTTGATGGATCTCCAGGATTCCAAAGGAACTGGAGCTACTGGGAATAGAAGATCCAGGACTTGTAAGGAAAACACAGACAAGGGCAATGCACACAAGAAAGTCACTGCTTATCCTAGACCATCTGCCTCTGCTCTCTTTACATGA

Coding sequence (CDS)

ATGGACCCATGTGGATTGCTTGAATTGATTCCATGGATTCTCGACGTGGTGAAATCAAATGGTGGAGAAAATCGCATCACAATTCTGCAAACCGAAGCCTTATCTGCTTCTGCTTCTGCTTCTGATTTGACCAGGGAGAAAACTGGTCAGGCTGTGGGATTTTGGGCCAGTTCCATGGGGATTTGCTTTAGCGCCAGAATTAAAGCTGTTAGCACATGCAGTACTGGTACAGCCATATTCTCTTGTGTTGGGAATTGGGATATTCTTGTTCTTTTGAATCTTTCTGGTATGGATATCTTACATTCAAGTCATGCAGGAGGAAACTTAAACAGCCTTGGCAGAGATGCAATCGATCTCTCTAGTTCTAGCAGCAACAGCAAGTTCTCATCAACATCAATTGCTCGGAGTGAGGATGAGATCTTGCAATCCTCAAATCTGAAGAGTTTTAGTTTTTCGGAACTTAAACTGGCCACTAGGAATTTCCGGCCTGATAGTTTGTTGGGGGAAGGTGGGTTTGGTTCGGTTTATAAAGGTTGGATCGATGAGCATTCATTTTCTGCTGCCAAGCCAGGGTCTGGCACGGTTGTTGCAGTGAAAAGGCTAAATCTAGATGGTCTACAAGGCCACAAGGAGTGGCTGGCTGAAGTCAATTTTCTGGGACAACTTCATCATTCTCACCTAGTGAGACTGATAGGATATTGTTTGGAAGACGAACATCGGTTGCTGGTGTATGAATTCCTTCCCCGAGGCAGCTTGGAGAACCATTTGTTTAGGAGAGGGTTGTACTTTCAACCTCTTTCATGGAGCCTTCGCTTGAAGATCGCTCTTGGCGCTGCAAAGGGACTGGCTTTCCTTCACAGTGATGAAAACAAAGTGATCTACCGTGATTTCAAGACATCAAATATACTCCTTGATTCGAACTACAATGCAAAGCTCTCTGATTTTGGCCTGGCCAAGGATGGACCTACGGGCGACAAAAGTCACGTCTCCACCAGAATTATGGGGACATATGGATATGCAGCTCCCGAGTATCTTGCTACAGGTCATCTTAGTGCCAAGAGTGACGTGTATAGTTTTGGGGTTGTTCTATTGGAGATGTTATCTGGTCGAAGAGCAATCGACAAGAACCGACCACCAGGGGAACAGAATCTGGTCGAGTGGGCCAAACCCTTCCTTGCTAATAAACGCAAGACTTTTCGTTTACTTGACGCACGTATTGAACGCAACTACTCCATGGAATCAGCTTTTAGAGTTGCAATCCTTGCATCACGTTGCCTATCTGCAGAACCCAAGTTCAGGCCGAGTATGAGCGAAATTGTAACAACGTTGATGGATCTCCAGGATTCCAAAGGAACTGGAGCTACTGGGAATAGAAGATCCAGGACTTGTAAGGAAAACACAGACAAGGGCAATGCACACAAGAAAGTCACTGCTTATCCTAGACCATCTGCCTCTGCTCTCTTTACATGA

Protein sequence

MDPCGLLELIPWILDVVKSNGGENRITILQTEALSASASASDLTREKTGQAVGFWASSMGICFSARIKAVSTCSTGTAIFSCVGNWDILVLLNLSGMDILHSSHAGGNLNSLGRDAIDLSSSSSNSKFSSTSIARSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIDEHSFSAAKPGSGTVVAVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLVRLIGYCLEDEHRLLVYEFLPRGSLENHLFRRGLYFQPLSWSLRLKIALGAAKGLAFLHSDENKVIYRDFKTSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSAKSDVYSFGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTFRLLDARIERNYSMESAFRVAILASRCLSAEPKFRPSMSEIVTTLMDLQDSKGTGATGNRRSRTCKENTDKGNAHKKVTAYPRPSASALFT
Homology
BLAST of Spg005756 vs. NCBI nr
Match: XP_038894946.1 (probable serine/threonine-protein kinase PBL10 [Benincasa hispida])

HSP 1 Score: 729.9 bits (1883), Expect = 1.4e-206
Identity = 377/431 (87.47%), Postives = 388/431 (90.02%), Query Frame = 0

Query: 59  MGICFSARIKAVSTCSTGTAIFSCVGNWDILVLLNLSGMDILHSSHAGGNLNSLGRDAID 118
           MGICFSARIKAVSTCST                              GGNL  + RDAI+
Sbjct: 1   MGICFSARIKAVSTCST------------------------------GGNLTGVSRDAIN 60

Query: 119 LSSSSSNSKFSSTSIARSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKG 178
           LSSSSSNSKFSSTSI RSEDEILQS+NLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKG
Sbjct: 61  LSSSSSNSKFSSTSIPRSEDEILQSTNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKG 120

Query: 179 WIDEHSFSAAKPGSGTVVAVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLVRLIGYCLEDE 238
           WIDEHSFSAAKPGSGTVVAVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLV+LIGYCLEDE
Sbjct: 121 WIDEHSFSAAKPGSGTVVAVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLVKLIGYCLEDE 180

Query: 239 HRLLVYEFLPRGSLENHLFRRGLYFQPLSWSLRLKIALGAAKGLAFLHSDENKVIYRDFK 298
           HRLLVYEFLPRGSLENHLFRRGLYFQPLSWSLRLKIALGAAKGLAFLHSDENKVIYRDFK
Sbjct: 181 HRLLVYEFLPRGSLENHLFRRGLYFQPLSWSLRLKIALGAAKGLAFLHSDENKVIYRDFK 240

Query: 299 TSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSAKSDVYS 358
           TSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSA+SDVYS
Sbjct: 241 TSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSARSDVYS 300

Query: 359 FGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTFRLLDARIERNYSMESAFRV 418
           FGVVLLEM+SGRRAIDKNRPPGEQNLVEWAKP LAN+RKTFRLLDARIERNYSMESAFR+
Sbjct: 301 FGVVLLEMISGRRAIDKNRPPGEQNLVEWAKPLLANRRKTFRLLDARIERNYSMESAFRL 360

Query: 419 AILASRCLSAEPKFRPSMSEIVTTLMDLQDSKGTGATGNRRSRTCKENTDKGNAHKKVTA 478
           AILASRCLS EPKFRPSM EIVTTL DLQDSKGTG  G RRSRTCK+NTD GN+HK+V A
Sbjct: 361 AILASRCLSVEPKFRPSMDEIVTTLNDLQDSKGTG--GTRRSRTCKQNTDNGNSHKEVAA 399

Query: 479 YPRPSASALFT 490
           YPRPSASALFT
Sbjct: 421 YPRPSASALFT 399

BLAST of Spg005756 vs. NCBI nr
Match: XP_022970537.1 (probable serine/threonine-protein kinase PBL10 isoform X1 [Cucurbita maxima])

HSP 1 Score: 723.4 bits (1866), Expect = 1.3e-204
Identity = 377/455 (82.86%), Postives = 396/455 (87.03%), Query Frame = 0

Query: 35  SASASASDLTREKTGQAVGFWASSMGICFSARIKAVSTCSTGTAIFSCVGNWDILVLLNL 94
           S S  A  L  E+TG  + F   SMG+C SARIKAVSTCST                   
Sbjct: 13  SDSLRALRLWSEQTGCEI-FGPVSMGVCLSARIKAVSTCST------------------- 72

Query: 95  SGMDILHSSHAGGNLNSLGRDAIDLSSSSSNSKFSSTSIARSEDEILQSSNLKSFSFSEL 154
                      GGN N + R+A DLSSSSSNSKFSS SI RSEDEILQSSNLKSFSFSEL
Sbjct: 73  -----------GGNSNGVSREANDLSSSSSNSKFSSISIPRSEDEILQSSNLKSFSFSEL 132

Query: 155 KLATRNFRPDSLLGEGGFGSVYKGWIDEHSFSAAKPGSGTVVAVKRLNLDGLQGHKEWLA 214
           KLATRNFRPDSLLGEGGFGSV+KGWIDEHSFSAAKPGSG VVAVKRLN+DG QGHKEWLA
Sbjct: 133 KLATRNFRPDSLLGEGGFGSVFKGWIDEHSFSAAKPGSGMVVAVKRLNVDGHQGHKEWLA 192

Query: 215 EVNFLGQLHHSHLVRLIGYCLEDEHRLLVYEFLPRGSLENHLFRRGLYFQPLSWSLRLKI 274
           EVNFLGQL H HLV+LIGYC EDEHRLLVYEFLPRGSLENHLF+RGLYFQPLSWSLRLKI
Sbjct: 193 EVNFLGQLRHPHLVKLIGYCFEDEHRLLVYEFLPRGSLENHLFKRGLYFQPLSWSLRLKI 252

Query: 275 ALGAAKGLAFLHSDENKVIYRDFKTSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRIM 334
           ALGAAKGLAFLH+DENKVIYRDFKTSNILLD+NYNAKLSDFGLAKDGPTGDKSHVSTR+M
Sbjct: 253 ALGAAKGLAFLHNDENKVIYRDFKTSNILLDTNYNAKLSDFGLAKDGPTGDKSHVSTRVM 312

Query: 335 GTYGYAAPEYLATGHLSAKSDVYSFGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLAN 394
           GTYGYAAPEYLATGHLSAKSD+YSFGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLAN
Sbjct: 313 GTYGYAAPEYLATGHLSAKSDLYSFGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLAN 372

Query: 395 KRKTFRLLDARIERNYSMESAFRVAILASRCLSAEPKFRPSMSEIVTTLMDLQDSKGTGA 454
           KRKT+RLLD RIERNYSMESAFRVAILASRCLSAEPKFRPSM EIVTTL DLQDSKGTGA
Sbjct: 373 KRKTYRLLDTRIERNYSMESAFRVAILASRCLSAEPKFRPSMDEIVTTLNDLQDSKGTGA 432

Query: 455 TGNRRSRTCKENTDKGNAHKKVTAYPRPSASALFT 490
           TGNRRSR+CK+NT+KGNAHK++TAYPRPSAS LFT
Sbjct: 433 TGNRRSRSCKQNTEKGNAHKEITAYPRPSASPLFT 436

BLAST of Spg005756 vs. NCBI nr
Match: KAG7019189.1 (putative serine/threonine-protein kinase PBL10 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 720.7 bits (1859), Expect = 8.5e-204
Identity = 369/431 (85.61%), Postives = 385/431 (89.33%), Query Frame = 0

Query: 59  MGICFSARIKAVSTCSTGTAIFSCVGNWDILVLLNLSGMDILHSSHAGGNLNSLGRDAID 118
           MG+C SARIKAVSTCST                              GGN N + R+A D
Sbjct: 1   MGVCLSARIKAVSTCST------------------------------GGNSNGVSREAND 60

Query: 119 LSSSSSNSKFSSTSIARSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKG 178
           LSSSSSNSKFSS SI RSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSV+KG
Sbjct: 61  LSSSSSNSKFSSISIPRSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVFKG 120

Query: 179 WIDEHSFSAAKPGSGTVVAVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLVRLIGYCLEDE 238
           WIDEHSFSAAKPGSG VVAVKRLN+DG QGHKEWLAEVNFLGQL H HLV+LIGYC EDE
Sbjct: 121 WIDEHSFSAAKPGSGMVVAVKRLNVDGHQGHKEWLAEVNFLGQLRHPHLVKLIGYCFEDE 180

Query: 239 HRLLVYEFLPRGSLENHLFRRGLYFQPLSWSLRLKIALGAAKGLAFLHSDENKVIYRDFK 298
           HRLLVYEFLPRGSLENHLF+RGLYFQPLSWSLRLKIALGAAKGLAFLH+DENKVIYRDFK
Sbjct: 181 HRLLVYEFLPRGSLENHLFKRGLYFQPLSWSLRLKIALGAAKGLAFLHNDENKVIYRDFK 240

Query: 299 TSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSAKSDVYS 358
           TSNILLD+NYNAKLSDFGLAKDGPTGDKSHVSTR+MGTYGYAAPEYLATGHLSAKSD+YS
Sbjct: 241 TSNILLDTNYNAKLSDFGLAKDGPTGDKSHVSTRVMGTYGYAAPEYLATGHLSAKSDLYS 300

Query: 359 FGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTFRLLDARIERNYSMESAFRV 418
           FGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKT+RLLD RIERNYSMESAFRV
Sbjct: 301 FGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTYRLLDTRIERNYSMESAFRV 360

Query: 419 AILASRCLSAEPKFRPSMSEIVTTLMDLQDSKGTGATGNRRSRTCKENTDKGNAHKKVTA 478
           AILASRCLSAEPKFRPSM EIVTTL DLQDSKGTGATGNRRSR+CK+NT+KGNAHK+VTA
Sbjct: 361 AILASRCLSAEPKFRPSMDEIVTTLNDLQDSKGTGATGNRRSRSCKQNTEKGNAHKEVTA 401

Query: 479 YPRPSASALFT 490
           YPRPSAS LFT
Sbjct: 421 YPRPSASPLFT 401

BLAST of Spg005756 vs. NCBI nr
Match: KAG6583426.1 (putative serine/threonine-protein kinase PBL10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 720.7 bits (1859), Expect = 8.5e-204
Identity = 369/431 (85.61%), Postives = 385/431 (89.33%), Query Frame = 0

Query: 59  MGICFSARIKAVSTCSTGTAIFSCVGNWDILVLLNLSGMDILHSSHAGGNLNSLGRDAID 118
           MG+C SARIKAVSTCST                              GGN N + R+A D
Sbjct: 1   MGVCLSARIKAVSTCST------------------------------GGNSNGVSREAND 60

Query: 119 LSSSSSNSKFSSTSIARSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKG 178
           LSSSSSNSKFSS SI RSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSV+KG
Sbjct: 61  LSSSSSNSKFSSISIPRSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVFKG 120

Query: 179 WIDEHSFSAAKPGSGTVVAVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLVRLIGYCLEDE 238
           WIDEHSFSAAKPGSG VVAVKRLN+DG QGHKEWLAEVNFLGQL H HLV+LIGYC EDE
Sbjct: 121 WIDEHSFSAAKPGSGMVVAVKRLNVDGHQGHKEWLAEVNFLGQLRHPHLVKLIGYCFEDE 180

Query: 239 HRLLVYEFLPRGSLENHLFRRGLYFQPLSWSLRLKIALGAAKGLAFLHSDENKVIYRDFK 298
           HRLLVYEFLPRGSLENHLF+RGLYFQPLSWSLRLKIALGAAKGLAFLH+DENKVIYRDFK
Sbjct: 181 HRLLVYEFLPRGSLENHLFKRGLYFQPLSWSLRLKIALGAAKGLAFLHNDENKVIYRDFK 240

Query: 299 TSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSAKSDVYS 358
           TSNILLD+NYNAKLSDFGLAKDGPTGDKSHVSTR+MGTYGYAAPEYLATGHLSAKSD+YS
Sbjct: 241 TSNILLDTNYNAKLSDFGLAKDGPTGDKSHVSTRVMGTYGYAAPEYLATGHLSAKSDLYS 300

Query: 359 FGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTFRLLDARIERNYSMESAFRV 418
           FGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKT+RLLD RIERNYSMESAFRV
Sbjct: 301 FGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTYRLLDTRIERNYSMESAFRV 360

Query: 419 AILASRCLSAEPKFRPSMSEIVTTLMDLQDSKGTGATGNRRSRTCKENTDKGNAHKKVTA 478
           AILASRCLSAEPKFRPSM EIVTTL DLQDSKGTGATGNRRSR+CK+NT+KGNAHK+VTA
Sbjct: 361 AILASRCLSAEPKFRPSMDEIVTTLNDLQDSKGTGATGNRRSRSCKQNTEKGNAHKEVTA 401

Query: 479 YPRPSASALFT 490
           YPRPSAS LFT
Sbjct: 421 YPRPSASPLFT 401

BLAST of Spg005756 vs. NCBI nr
Match: XP_022970539.1 (probable serine/threonine-protein kinase PBL10 isoform X3 [Cucurbita maxima])

HSP 1 Score: 720.3 bits (1858), Expect = 1.1e-203
Identity = 368/431 (85.38%), Postives = 385/431 (89.33%), Query Frame = 0

Query: 59  MGICFSARIKAVSTCSTGTAIFSCVGNWDILVLLNLSGMDILHSSHAGGNLNSLGRDAID 118
           MG+C SARIKAVSTCST                              GGN N + R+A D
Sbjct: 1   MGVCLSARIKAVSTCST------------------------------GGNSNGVSREAND 60

Query: 119 LSSSSSNSKFSSTSIARSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKG 178
           LSSSSSNSKFSS SI RSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSV+KG
Sbjct: 61  LSSSSSNSKFSSISIPRSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVFKG 120

Query: 179 WIDEHSFSAAKPGSGTVVAVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLVRLIGYCLEDE 238
           WIDEHSFSAAKPGSG VVAVKRLN+DG QGHKEWLAEVNFLGQL H HLV+LIGYC EDE
Sbjct: 121 WIDEHSFSAAKPGSGMVVAVKRLNVDGHQGHKEWLAEVNFLGQLRHPHLVKLIGYCFEDE 180

Query: 239 HRLLVYEFLPRGSLENHLFRRGLYFQPLSWSLRLKIALGAAKGLAFLHSDENKVIYRDFK 298
           HRLLVYEFLPRGSLENHLF+RGLYFQPLSWSLRLKIALGAAKGLAFLH+DENKVIYRDFK
Sbjct: 181 HRLLVYEFLPRGSLENHLFKRGLYFQPLSWSLRLKIALGAAKGLAFLHNDENKVIYRDFK 240

Query: 299 TSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSAKSDVYS 358
           TSNILLD+NYNAKLSDFGLAKDGPTGDKSHVSTR+MGTYGYAAPEYLATGHLSAKSD+YS
Sbjct: 241 TSNILLDTNYNAKLSDFGLAKDGPTGDKSHVSTRVMGTYGYAAPEYLATGHLSAKSDLYS 300

Query: 359 FGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTFRLLDARIERNYSMESAFRV 418
           FGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKT+RLLD RIERNYSMESAFRV
Sbjct: 301 FGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTYRLLDTRIERNYSMESAFRV 360

Query: 419 AILASRCLSAEPKFRPSMSEIVTTLMDLQDSKGTGATGNRRSRTCKENTDKGNAHKKVTA 478
           AILASRCLSAEPKFRPSM EIVTTL DLQDSKGTGATGNRRSR+CK+NT+KGNAHK++TA
Sbjct: 361 AILASRCLSAEPKFRPSMDEIVTTLNDLQDSKGTGATGNRRSRSCKQNTEKGNAHKEITA 401

Query: 479 YPRPSASALFT 490
           YPRPSAS LFT
Sbjct: 421 YPRPSASPLFT 401

BLAST of Spg005756 vs. ExPASy Swiss-Prot
Match: Q06548 (Probable serine/threonine-protein kinase PBL9 OS=Arabidopsis thaliana OX=3702 GN=PBL9 PE=1 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 1.7e-146
Identity = 270/391 (69.05%), Postives = 312/391 (79.80%), Query Frame = 0

Query: 115 DAIDLSSSSSNSKFSSTSI---ARSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGG 174
           DA D+   S  SK SS S+    R+E EILQS NLKSFSF+ELK ATRNFRPDS+LGEGG
Sbjct: 21  DAKDI--GSLGSKASSVSVRPSPRTEGEILQSPNLKSFSFAELKSATRNFRPDSVLGEGG 80

Query: 175 FGSVYKGWIDEHSFSAAKPGSGTVVAVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLVRLI 234
           FG V+KGWIDE S +A++PG+G V+AVK+LN DG QGH+EWLAEVN+LGQ  H HLV+LI
Sbjct: 81  FGCVFKGWIDEKSLTASRPGTGLVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHRHLVKLI 140

Query: 235 GYCLEDEHRLLVYEFLPRGSLENHLFRRGLYFQPLSWSLRLKIALGAAKGLAFLHSDENK 294
           GYCLEDEHRLLVYEF+PRGSLENHLFRRGLYFQPLSW LRLK+ALGAAKGLAFLHS E +
Sbjct: 141 GYCLEDEHRLLVYEFMPRGSLENHLFRRGLYFQPLSWKLRLKVALGAAKGLAFLHSSETR 200

Query: 295 VIYRDFKTSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLS 354
           VIYRDFKTSNILLDS YNAKLSDFGLAKDGP GDKSHVSTR+MGT+GYAAPEYLATGHL+
Sbjct: 201 VIYRDFKTSNILLDSEYNAKLSDFGLAKDGPIGDKSHVSTRVMGTHGYAAPEYLATGHLT 260

Query: 355 AKSDVYSFGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTFRLLDARIERNYS 414
            KSDVYSFGVVLLE+LSGRRA+DKNRP GE+NLVEWAKP+L NKRK FR++D R++  YS
Sbjct: 261 TKSDVYSFGVVLLELLSGRRAVDKNRPSGERNLVEWAKPYLVNKRKIFRVIDNRLQDQYS 320

Query: 415 MESAFRVAILASRCLSAEPKFRPSMSEIVTTLMDLQD-SKGTGATGNRRSRTCKENTD-- 474
           ME A +VA L+ RCL+ E K RP+MSE+V+ L  +Q  +   G   ++  R  +  +D  
Sbjct: 321 MEEACKVATLSLRCLTTEIKLRPNMSEVVSHLEHIQSLNAAIGGNMDKTDRRMRRRSDSV 380

Query: 475 ---KGNA--------HKKVTAYPRPSASALF 489
              K NA           V AYPRPSAS L+
Sbjct: 381 VSKKVNAGFARQTAVGSTVVAYPRPSASPLY 409

BLAST of Spg005756 vs. ExPASy Swiss-Prot
Match: P46573 (Probable serine/threonine-protein kinase PBL10 OS=Arabidopsis thaliana OX=3702 GN=PBL10 PE=1 SV=2)

HSP 1 Score: 516.5 bits (1329), Expect = 3.2e-145
Identity = 279/445 (62.70%), Postives = 323/445 (72.58%), Query Frame = 0

Query: 59  MGICFSARIKAVSTCSTGTAIFSCVGNWDILVLLNLSGMDILHSSHAGGNLNSLGRDAID 118
           MGIC SA+IKAVS                                  G +   +  +A D
Sbjct: 1   MGICLSAQIKAVS---------------------------------PGASPKYMSSEAND 60

Query: 119 LSSSSSNSKFSSTSIARSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKG 178
            S  S +S  S  +  R+E EILQS NLKSF+F+ELK ATRNFRPDS+LGEGGFGSV+KG
Sbjct: 61  -SLGSKSSSVSIRTNPRTEGEILQSPNLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKG 120

Query: 179 WIDEHSFSAAKPGSGTVVAVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLVRLIGYCLEDE 238
           WIDE + +A+KPG+G V+AVK+LN DG QGH+EWLAEVN+LGQ  H +LV+LIGYCLEDE
Sbjct: 121 WIDEQTLTASKPGTGVVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHPNLVKLIGYCLEDE 180

Query: 239 HRLLVYEFLPRGSLENHLFRRGLYFQPLSWSLRLKIALGAAKGLAFLHSDENKVIYRDFK 298
           HRLLVYEF+PRGSLENHLFRRG YFQPLSW+LRLK+ALGAAKGLAFLH+ E  VIYRDFK
Sbjct: 181 HRLLVYEFMPRGSLENHLFRRGSYFQPLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFK 240

Query: 299 TSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSAKSDVYS 358
           TSNILLDS YNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHL+ KSDVYS
Sbjct: 241 TSNILLDSEYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDVYS 300

Query: 359 FGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTFRLLDARIERNYSMESAFRV 418
           +GVVLLE+LSGRRA+DKNRPPGEQ LVEWA+P LANKRK FR++D R++  YSME A +V
Sbjct: 301 YGVVLLEVLSGRRAVDKNRPPGEQKLVEWARPLLANKRKLFRVIDNRLQDQYSMEEACKV 360

Query: 419 AILASRCLSAEPKFRPSMSEIVTTLMDLQDSKGTGATG----NRRSR------TCKENTD 478
           A LA RCL+ E K RP+M+E+V+ L  +Q     G        RR R         +  +
Sbjct: 361 ATLALRCLTFEIKLRPNMNEVVSHLEHIQTLNEAGGRNIDMVQRRMRRRSDSVAINQKPN 411

Query: 479 KGNAHKK-----VTAYPRPSASALF 489
            G A +       TAYPRPS S LF
Sbjct: 421 AGFARQTAVGVIATAYPRPSDSPLF 411

BLAST of Spg005756 vs. ExPASy Swiss-Prot
Match: Q65XV8 (Receptor-like cytoplasmic kinase 176 OS=Oryza sativa subsp. japonica OX=39947 GN=RLCK176 PE=1 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 1.6e-141
Identity = 249/371 (67.12%), Postives = 307/371 (82.75%), Query Frame = 0

Query: 120 SSSSSNSKFSSTSI---ARSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVY 179
           S  +SNS  S+ S+    RSEDEIL+++N+K+F+F+EL+ ATRNFRPDS+LGEGGFGSV+
Sbjct: 26  SKYASNSSVSAASVPPTPRSEDEILEAANVKAFAFNELRTATRNFRPDSVLGEGGFGSVF 85

Query: 180 KGWIDEHSFSAAKPGSGTVVAVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLVRLIGYCLE 239
           KGWIDE + +  KPG+G V+AVK+LN +G QGH+EWLAEVN+LGQL H +LVRL+GYC+E
Sbjct: 86  KGWIDEKTLAPTKPGTGMVIAVKKLNQEGHQGHREWLAEVNYLGQLSHPYLVRLVGYCVE 145

Query: 240 DEHRLLVYEFLPRGSLENHLFRRGLYFQPLSWSLRLKIALGAAKGLAFLHSDENKVIYRD 299
           DE RLLVYEF+PRGSLENHLFRR  +FQPLSW+LR+KIALGAAKGLAFLHSD+ KVIYRD
Sbjct: 146 DEQRLLVYEFMPRGSLENHLFRRSTHFQPLSWNLRMKIALGAAKGLAFLHSDKVKVIYRD 205

Query: 300 FKTSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSAKSDV 359
           FKTSN+LLD+NY+AKLSDFGLAKDGPTGDKSHVSTR+MGTYGYAAPEYLATGHL+ KSDV
Sbjct: 206 FKTSNVLLDANYDAKLSDFGLAKDGPTGDKSHVSTRVMGTYGYAAPEYLATGHLTTKSDV 265

Query: 360 YSFGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTFRLLDARIERNYSMESAF 419
           YSFGVVLLEMLSGRRA+DKNRP GE NLVEWA+P+L +KR+ FR+LDAR+   YS+  A 
Sbjct: 266 YSFGVVLLEMLSGRRALDKNRPTGEHNLVEWARPYLMSKRRIFRILDARLGGQYSLAKAQ 325

Query: 420 RVAILASRCLSAEPKFRPSMSEIVTTLMDLQDSKGTGATGNRRSRTCKENTDKGNAHKKV 479
           + A LA +C+S E K RP+M ++V  L  LQDSK TGA    + ++  +N   G+   K 
Sbjct: 326 KAATLALQCISVEAKNRPNMEQVVAVLEQLQDSKETGANPQLQKKSSSKNA--GSNGSKP 385

Query: 480 TAYPRPSASAL 488
           ++  +P+ + L
Sbjct: 386 SSKGKPANARL 394

BLAST of Spg005756 vs. ExPASy Swiss-Prot
Match: P43293 (Probable serine/threonine-protein kinase PBL11 OS=Arabidopsis thaliana OX=3702 GN=PBL11 PE=1 SV=2)

HSP 1 Score: 472.6 bits (1215), Expect = 5.3e-132
Identity = 235/367 (64.03%), Postives = 294/367 (80.11%), Query Frame = 0

Query: 118 DLSSSSSNSKFSSTSIARSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYK 177
           D S  SS + FS   + R+E EILQ++NLK+FS SELK ATRNFRPDS++GEGGFG V+K
Sbjct: 27  DGSKGSSTASFS--YMPRTEGEILQNANLKNFSLSELKSATRNFRPDSVVGEGGFGCVFK 86

Query: 178 GWIDEHSFSAAKPGSGTVVAVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLVRLIGYCLED 237
           GWIDE S + +KPG+G V+AVKRLN +G QGH+EWLAE+N+LGQL H +LV+LIGYCLE+
Sbjct: 87  GWIDESSLAPSKPGTGIVIAVKRLNQEGFQGHREWLAEINYLGQLDHPNLVKLIGYCLEE 146

Query: 238 EHRLLVYEFLPRGSLENHLFRRGLYFQPLSWSLRLKIALGAAKGLAFLHSDENKVIYRDF 297
           EHRLLVYEF+ RGSLENHLFRRG ++QPLSW+ R+++ALGAA+GLAFLH+ + +VIYRDF
Sbjct: 147 EHRLLVYEFMTRGSLENHLFRRGTFYQPLSWNTRVRMALGAARGLAFLHNAQPQVIYRDF 206

Query: 298 KTSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSAKSDVY 357
           K SNILLDSNYNAKLSDFGLA+DGP GD SHVSTR+MGT GYAAPEYLATGHLS KSDVY
Sbjct: 207 KASNILLDSNYNAKLSDFGLARDGPMGDNSHVSTRVMGTQGYAAPEYLATGHLSVKSDVY 266

Query: 358 SFGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTFRLLDARIERNYSMESAFR 417
           SFGVVLLE+LSGRRAIDKN+P GE NLV+WA+P+L NKR+  R++D R++  YS+  A +
Sbjct: 267 SFGVVLLELLSGRRAIDKNQPVGEHNLVDWARPYLTNKRRLLRVMDPRLQGQYSLTRALK 326

Query: 418 VAILASRCLSAEPKFRPSMSEIVTTLMDLQDSKGTGATGNRRSRTCKENTDK-GNAHKKV 477
           +A+LA  C+S + K RP+M+EIV T+ +L   K      ++  +  + + D   N   + 
Sbjct: 327 IAVLALDCISIDAKSRPTMNEIVKTMEELHIQK----EASKEQQNPQISIDNIINKSPQA 386

Query: 478 TAYPRPS 484
             YPRPS
Sbjct: 387 VNYPRPS 387

BLAST of Spg005756 vs. ExPASy Swiss-Prot
Match: O48814 (Serine/threonine-protein kinase BIK1 OS=Arabidopsis thaliana OX=3702 GN=BIK1 PE=1 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 3.0e-127
Identity = 236/425 (55.53%), Postives = 300/425 (70.59%), Query Frame = 0

Query: 59  MGICFSARIKAVSTCSTGTAIFSCVGNWDILVLLNLSGMDILHSSHAGGNLNSLGRDAID 118
           MG CFS+R+KA                            DI H        N    D   
Sbjct: 1   MGSCFSSRVKA----------------------------DIFH--------NGKSSDLYG 60

Query: 119 LSSSSSNSKFSSTSIARSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKG 178
           LS SS  S  +  +  ++E EIL S+ +KSF+F+ELKLATRNFRPDS++GEGGFG V+KG
Sbjct: 61  LSLSSRKSSSTVAAAQKTEGEILSSTPVKSFTFNELKLATRNFRPDSVIGEGGFGCVFKG 120

Query: 179 WIDEHSFSAAKPGSGTVVAVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLVRLIGYCLEDE 238
           W+DE + +  KPG+G V+AVK+LN +G QGH+EWL E+N+LGQL H +LV+LIGYCLEDE
Sbjct: 121 WLDESTLTPTKPGTGLVIAVKKLNQEGFQGHREWLTEINYLGQLSHPNLVKLIGYCLEDE 180

Query: 239 HRLLVYEFLPRGSLENHLFRRGLYFQPLSWSLRLKIALGAAKGLAFLHSDENKVIYRDFK 298
           HRLLVYEF+ +GSLENHLFRRG YF+PL W LR+ +AL AAKGLAFLHSD  KVIYRD K
Sbjct: 181 HRLLVYEFMQKGSLENHLFRRGAYFKPLPWFLRVNVALDAAKGLAFLHSDPVKVIYRDIK 240

Query: 299 TSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSAKSDVYS 358
            SNILLD++YNAKLSDFGLA+DGP GD S+VSTR+MGTYGYAAPEY+++GHL+A+SDVYS
Sbjct: 241 ASNILLDADYNAKLSDFGLARDGPMGDLSYVSTRVMGTYGYAAPEYMSSGHLNARSDVYS 300

Query: 359 FGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTFRLLDARIERNYSMESAFRV 418
           FGV+LLE+LSG+RA+D NRP  E+NLV+WA+P+L +KRK   ++D R++  Y  E A R+
Sbjct: 301 FGVLLLEILSGKRALDHNRPAKEENLVDWARPYLTSKRKVLLIVDNRLDTQYLPEEAVRM 360

Query: 419 AILASRCLSAEPKFRPSMSEIVTTLMDLQDSKGTGATGNRRSRTCKENTDKG---NAHKK 478
           A +A +CLS EPK RP+M ++V  L  LQD+ G  +  N    T K     G   ++ K+
Sbjct: 361 ASVAVQCLSFEPKSRPTMDQVVRALQQLQDNLGKPSQTNPVKDTKKLGFKTGTTKSSEKR 389

Query: 479 VTAYP 481
            T  P
Sbjct: 421 FTQKP 389

BLAST of Spg005756 vs. ExPASy TrEMBL
Match: A0A6J1HZE0 (probable serine/threonine-protein kinase PBL10 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111469485 PE=3 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 6.4e-205
Identity = 377/455 (82.86%), Postives = 396/455 (87.03%), Query Frame = 0

Query: 35  SASASASDLTREKTGQAVGFWASSMGICFSARIKAVSTCSTGTAIFSCVGNWDILVLLNL 94
           S S  A  L  E+TG  + F   SMG+C SARIKAVSTCST                   
Sbjct: 13  SDSLRALRLWSEQTGCEI-FGPVSMGVCLSARIKAVSTCST------------------- 72

Query: 95  SGMDILHSSHAGGNLNSLGRDAIDLSSSSSNSKFSSTSIARSEDEILQSSNLKSFSFSEL 154
                      GGN N + R+A DLSSSSSNSKFSS SI RSEDEILQSSNLKSFSFSEL
Sbjct: 73  -----------GGNSNGVSREANDLSSSSSNSKFSSISIPRSEDEILQSSNLKSFSFSEL 132

Query: 155 KLATRNFRPDSLLGEGGFGSVYKGWIDEHSFSAAKPGSGTVVAVKRLNLDGLQGHKEWLA 214
           KLATRNFRPDSLLGEGGFGSV+KGWIDEHSFSAAKPGSG VVAVKRLN+DG QGHKEWLA
Sbjct: 133 KLATRNFRPDSLLGEGGFGSVFKGWIDEHSFSAAKPGSGMVVAVKRLNVDGHQGHKEWLA 192

Query: 215 EVNFLGQLHHSHLVRLIGYCLEDEHRLLVYEFLPRGSLENHLFRRGLYFQPLSWSLRLKI 274
           EVNFLGQL H HLV+LIGYC EDEHRLLVYEFLPRGSLENHLF+RGLYFQPLSWSLRLKI
Sbjct: 193 EVNFLGQLRHPHLVKLIGYCFEDEHRLLVYEFLPRGSLENHLFKRGLYFQPLSWSLRLKI 252

Query: 275 ALGAAKGLAFLHSDENKVIYRDFKTSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRIM 334
           ALGAAKGLAFLH+DENKVIYRDFKTSNILLD+NYNAKLSDFGLAKDGPTGDKSHVSTR+M
Sbjct: 253 ALGAAKGLAFLHNDENKVIYRDFKTSNILLDTNYNAKLSDFGLAKDGPTGDKSHVSTRVM 312

Query: 335 GTYGYAAPEYLATGHLSAKSDVYSFGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLAN 394
           GTYGYAAPEYLATGHLSAKSD+YSFGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLAN
Sbjct: 313 GTYGYAAPEYLATGHLSAKSDLYSFGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLAN 372

Query: 395 KRKTFRLLDARIERNYSMESAFRVAILASRCLSAEPKFRPSMSEIVTTLMDLQDSKGTGA 454
           KRKT+RLLD RIERNYSMESAFRVAILASRCLSAEPKFRPSM EIVTTL DLQDSKGTGA
Sbjct: 373 KRKTYRLLDTRIERNYSMESAFRVAILASRCLSAEPKFRPSMDEIVTTLNDLQDSKGTGA 432

Query: 455 TGNRRSRTCKENTDKGNAHKKVTAYPRPSASALFT 490
           TGNRRSR+CK+NT+KGNAHK++TAYPRPSAS LFT
Sbjct: 433 TGNRRSRSCKQNTEKGNAHKEITAYPRPSASPLFT 436

BLAST of Spg005756 vs. ExPASy TrEMBL
Match: A0A6J1I5T6 (probable serine/threonine-protein kinase PBL10 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111469485 PE=3 SV=1)

HSP 1 Score: 720.3 bits (1858), Expect = 5.4e-204
Identity = 368/431 (85.38%), Postives = 385/431 (89.33%), Query Frame = 0

Query: 59  MGICFSARIKAVSTCSTGTAIFSCVGNWDILVLLNLSGMDILHSSHAGGNLNSLGRDAID 118
           MG+C SARIKAVSTCST                              GGN N + R+A D
Sbjct: 1   MGVCLSARIKAVSTCST------------------------------GGNSNGVSREAND 60

Query: 119 LSSSSSNSKFSSTSIARSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKG 178
           LSSSSSNSKFSS SI RSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSV+KG
Sbjct: 61  LSSSSSNSKFSSISIPRSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVFKG 120

Query: 179 WIDEHSFSAAKPGSGTVVAVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLVRLIGYCLEDE 238
           WIDEHSFSAAKPGSG VVAVKRLN+DG QGHKEWLAEVNFLGQL H HLV+LIGYC EDE
Sbjct: 121 WIDEHSFSAAKPGSGMVVAVKRLNVDGHQGHKEWLAEVNFLGQLRHPHLVKLIGYCFEDE 180

Query: 239 HRLLVYEFLPRGSLENHLFRRGLYFQPLSWSLRLKIALGAAKGLAFLHSDENKVIYRDFK 298
           HRLLVYEFLPRGSLENHLF+RGLYFQPLSWSLRLKIALGAAKGLAFLH+DENKVIYRDFK
Sbjct: 181 HRLLVYEFLPRGSLENHLFKRGLYFQPLSWSLRLKIALGAAKGLAFLHNDENKVIYRDFK 240

Query: 299 TSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSAKSDVYS 358
           TSNILLD+NYNAKLSDFGLAKDGPTGDKSHVSTR+MGTYGYAAPEYLATGHLSAKSD+YS
Sbjct: 241 TSNILLDTNYNAKLSDFGLAKDGPTGDKSHVSTRVMGTYGYAAPEYLATGHLSAKSDLYS 300

Query: 359 FGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTFRLLDARIERNYSMESAFRV 418
           FGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKT+RLLD RIERNYSMESAFRV
Sbjct: 301 FGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTYRLLDTRIERNYSMESAFRV 360

Query: 419 AILASRCLSAEPKFRPSMSEIVTTLMDLQDSKGTGATGNRRSRTCKENTDKGNAHKKVTA 478
           AILASRCLSAEPKFRPSM EIVTTL DLQDSKGTGATGNRRSR+CK+NT+KGNAHK++TA
Sbjct: 361 AILASRCLSAEPKFRPSMDEIVTTLNDLQDSKGTGATGNRRSRSCKQNTEKGNAHKEITA 401

Query: 479 YPRPSASALFT 490
           YPRPSAS LFT
Sbjct: 421 YPRPSASPLFT 401

BLAST of Spg005756 vs. ExPASy TrEMBL
Match: A0A6J1HLY9 (probable serine/threonine-protein kinase PBL10 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111464795 PE=3 SV=1)

HSP 1 Score: 718.0 bits (1852), Expect = 2.7e-203
Identity = 368/431 (85.38%), Postives = 384/431 (89.10%), Query Frame = 0

Query: 59  MGICFSARIKAVSTCSTGTAIFSCVGNWDILVLLNLSGMDILHSSHAGGNLNSLGRDAID 118
           MG+C SARIKAVSTCST                              GGN N + R+A D
Sbjct: 1   MGVCLSARIKAVSTCST------------------------------GGNSNGVSREAND 60

Query: 119 LSSSSSNSKFSSTSIARSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKG 178
           LSSSSSNSKFSS SI RSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSV+KG
Sbjct: 61  LSSSSSNSKFSSISIPRSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVFKG 120

Query: 179 WIDEHSFSAAKPGSGTVVAVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLVRLIGYCLEDE 238
           WIDEHSFSAAKPGSG VVAVKRLN+DG QGHKEWLAEVNFLGQL H HLV+LIGYC EDE
Sbjct: 121 WIDEHSFSAAKPGSGMVVAVKRLNVDGHQGHKEWLAEVNFLGQLRHPHLVKLIGYCFEDE 180

Query: 239 HRLLVYEFLPRGSLENHLFRRGLYFQPLSWSLRLKIALGAAKGLAFLHSDENKVIYRDFK 298
           HRLLVYEFLPRGSLENHLF+RGLYFQPLSWSLRLKIALGAAKGLAFLH+DENKVIYRDFK
Sbjct: 181 HRLLVYEFLPRGSLENHLFKRGLYFQPLSWSLRLKIALGAAKGLAFLHNDENKVIYRDFK 240

Query: 299 TSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSAKSDVYS 358
           TSNILLD+NYNAKLSDFGLAKDGPTG KSHVSTR+MGTYGYAAPEYLATGHLSAKSD+YS
Sbjct: 241 TSNILLDTNYNAKLSDFGLAKDGPTGGKSHVSTRVMGTYGYAAPEYLATGHLSAKSDLYS 300

Query: 359 FGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTFRLLDARIERNYSMESAFRV 418
           FGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKT+RLLD RIERNYSMESAFRV
Sbjct: 301 FGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTYRLLDTRIERNYSMESAFRV 360

Query: 419 AILASRCLSAEPKFRPSMSEIVTTLMDLQDSKGTGATGNRRSRTCKENTDKGNAHKKVTA 478
           AILASRCLSAEPKFRPSM EIVTTL DLQDSKGTGATGNRRSR+CK+NT+KGNAHK+VTA
Sbjct: 361 AILASRCLSAEPKFRPSMDEIVTTLNDLQDSKGTGATGNRRSRSCKQNTEKGNAHKEVTA 401

Query: 479 YPRPSASALFT 490
           YPRPSAS LFT
Sbjct: 421 YPRPSASPLFT 401

BLAST of Spg005756 vs. ExPASy TrEMBL
Match: A0A5A7VI25 (Protein kinase APK1B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold352G00630 PE=3 SV=1)

HSP 1 Score: 715.3 bits (1845), Expect = 1.7e-202
Identity = 373/431 (86.54%), Postives = 385/431 (89.33%), Query Frame = 0

Query: 59  MGICFSARIKAVSTCSTGTAIFSCVGNWDILVLLNLSGMDILHSSHAGGNLNSLGRDAID 118
           MGICFSA+IKAVSTCST                              GGNLNS+ RDAID
Sbjct: 1   MGICFSAKIKAVSTCST------------------------------GGNLNSMSRDAID 60

Query: 119 LSSSSSNSKFSSTSIARSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKG 178
           LSSSS NSKFSSTSI RSEDEIL SSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKG
Sbjct: 61  LSSSSINSKFSSTSIPRSEDEILHSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKG 120

Query: 179 WIDEHSFSAAKPGSGTVVAVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLVRLIGYCLEDE 238
           WIDEHSFSAAKPGSGTVVAVKRLNLDGLQGHKEWLAEV FLGQLHHSHLV+LIGYCLEDE
Sbjct: 121 WIDEHSFSAAKPGSGTVVAVKRLNLDGLQGHKEWLAEVTFLGQLHHSHLVKLIGYCLEDE 180

Query: 239 HRLLVYEFLPRGSLENHLFRRGLYFQPLSWSLRLKIALGAAKGLAFLHSDENKVIYRDFK 298
           HRLLVYEFLPRGSLENHLFRRGLYFQPLSWSLRLKIALGAAKGLAFLHS+ENKVIYRDFK
Sbjct: 181 HRLLVYEFLPRGSLENHLFRRGLYFQPLSWSLRLKIALGAAKGLAFLHSEENKVIYRDFK 240

Query: 299 TSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSAKSDVYS 358
           TSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSAKSDVYS
Sbjct: 241 TSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSAKSDVYS 300

Query: 359 FGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTFRLLDARIERNYSMESAFRV 418
           FGVVLLEM+SGRRAIDKNRP GEQNLVEWAKP LAN+RKTFRLLDARIERNYSMESAFR+
Sbjct: 301 FGVVLLEMISGRRAIDKNRPQGEQNLVEWAKPLLANRRKTFRLLDARIERNYSMESAFRL 360

Query: 419 AILASRCLSAEPKFRPSMSEIVTTLMDLQDSKGTGATGNRRSRTCKENTDKGNAHKKVTA 478
           A+LASRCLSAEP+FRP+M EIVT L DLQDSKGTG  G RRSRTCK+NTD GN  K+VTA
Sbjct: 361 AVLASRCLSAEPRFRPNMDEIVTMLNDLQDSKGTG--GPRRSRTCKQNTDSGN--KEVTA 397

Query: 479 YPRPSASALFT 490
           YPRPSASALFT
Sbjct: 421 YPRPSASALFT 397

BLAST of Spg005756 vs. ExPASy TrEMBL
Match: A0A1S3C567 (protein kinase APK1B, chloroplastic-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497157 PE=3 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 3.0e-202
Identity = 373/431 (86.54%), Postives = 385/431 (89.33%), Query Frame = 0

Query: 59  MGICFSARIKAVSTCSTGTAIFSCVGNWDILVLLNLSGMDILHSSHAGGNLNSLGRDAID 118
           MGICFSA+IKAVSTCST                              GGNLNS+ RDAID
Sbjct: 1   MGICFSAKIKAVSTCST------------------------------GGNLNSMSRDAID 60

Query: 119 LSSSSSNSKFSSTSIARSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKG 178
           LSSSS NSKFSSTSI RSEDEIL SSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKG
Sbjct: 61  LSSSSINSKFSSTSIPRSEDEILHSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKG 120

Query: 179 WIDEHSFSAAKPGSGTVVAVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLVRLIGYCLEDE 238
           WIDEHSFSAAKPGSGTVVAVKRLNLDGLQGHKEWLAEV FLGQLHHSHLV+LIGYCLEDE
Sbjct: 121 WIDEHSFSAAKPGSGTVVAVKRLNLDGLQGHKEWLAEVTFLGQLHHSHLVKLIGYCLEDE 180

Query: 239 HRLLVYEFLPRGSLENHLFRRGLYFQPLSWSLRLKIALGAAKGLAFLHSDENKVIYRDFK 298
           HRLLVYEFLPRGSLENHLFRRGLYFQPLSWSLRLKIALGAAKGLAFLHS+ENKVIYRDFK
Sbjct: 181 HRLLVYEFLPRGSLENHLFRRGLYFQPLSWSLRLKIALGAAKGLAFLHSEENKVIYRDFK 240

Query: 299 TSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSAKSDVYS 358
           TSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSAKSDVYS
Sbjct: 241 TSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSAKSDVYS 300

Query: 359 FGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTFRLLDARIERNYSMESAFRV 418
           FGVVLLEM+SGRRAIDKNRP GEQNLVEWAKP LAN+RKTFRLLDARIERNYSMESAFR+
Sbjct: 301 FGVVLLEMISGRRAIDKNRPQGEQNLVEWAKPLLANRRKTFRLLDARIERNYSMESAFRL 360

Query: 419 AILASRCLSAEPKFRPSMSEIVTTLMDLQDSKGTGATGNRRSRTCKENTDKGNAHKKVTA 478
           A+LASRCLSAEP+FRP+M EIVT L DLQDSKGTG  G RRSRTCK+NTD GN  K+VTA
Sbjct: 361 AVLASRCLSAEPRFRPNMDEIVTMLNDLQDSKGTG--GPRRSRTCKQNTDGGN--KEVTA 397

Query: 479 YPRPSASALFT 490
           YPRPSASALFT
Sbjct: 421 YPRPSASALFT 397

BLAST of Spg005756 vs. TAIR 10
Match: AT1G07570.2 (Protein kinase superfamily protein )

HSP 1 Score: 520.8 bits (1340), Expect = 1.2e-147
Identity = 270/391 (69.05%), Postives = 312/391 (79.80%), Query Frame = 0

Query: 115 DAIDLSSSSSNSKFSSTSI---ARSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGG 174
           DA D+   S  SK SS S+    R+E EILQS NLKSFSF+ELK ATRNFRPDS+LGEGG
Sbjct: 21  DAKDI--GSLGSKASSVSVRPSPRTEGEILQSPNLKSFSFAELKSATRNFRPDSVLGEGG 80

Query: 175 FGSVYKGWIDEHSFSAAKPGSGTVVAVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLVRLI 234
           FG V+KGWIDE S +A++PG+G V+AVK+LN DG QGH+EWLAEVN+LGQ  H HLV+LI
Sbjct: 81  FGCVFKGWIDEKSLTASRPGTGLVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHRHLVKLI 140

Query: 235 GYCLEDEHRLLVYEFLPRGSLENHLFRRGLYFQPLSWSLRLKIALGAAKGLAFLHSDENK 294
           GYCLEDEHRLLVYEF+PRGSLENHLFRRGLYFQPLSW LRLK+ALGAAKGLAFLHS E +
Sbjct: 141 GYCLEDEHRLLVYEFMPRGSLENHLFRRGLYFQPLSWKLRLKVALGAAKGLAFLHSSETR 200

Query: 295 VIYRDFKTSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLS 354
           VIYRDFKTSNILLDS YNAKLSDFGLAKDGP GDKSHVSTR+MGT+GYAAPEYLATGHL+
Sbjct: 201 VIYRDFKTSNILLDSEYNAKLSDFGLAKDGPIGDKSHVSTRVMGTHGYAAPEYLATGHLT 260

Query: 355 AKSDVYSFGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTFRLLDARIERNYS 414
            KSDVYSFGVVLLE+LSGRRA+DKNRP GE+NLVEWAKP+L NKRK FR++D R++  YS
Sbjct: 261 TKSDVYSFGVVLLELLSGRRAVDKNRPSGERNLVEWAKPYLVNKRKIFRVIDNRLQDQYS 320

Query: 415 MESAFRVAILASRCLSAEPKFRPSMSEIVTTLMDLQD-SKGTGATGNRRSRTCKENTD-- 474
           ME A +VA L+ RCL+ E K RP+MSE+V+ L  +Q  +   G   ++  R  +  +D  
Sbjct: 321 MEEACKVATLSLRCLTTEIKLRPNMSEVVSHLEHIQSLNAAIGGNMDKTDRRMRRRSDSV 380

Query: 475 ---KGNA--------HKKVTAYPRPSASALF 489
              K NA           V AYPRPSAS L+
Sbjct: 381 VSKKVNAGFARQTAVGSTVVAYPRPSASPLY 409

BLAST of Spg005756 vs. TAIR 10
Match: AT1G07570.1 (Protein kinase superfamily protein )

HSP 1 Score: 520.8 bits (1340), Expect = 1.2e-147
Identity = 270/391 (69.05%), Postives = 312/391 (79.80%), Query Frame = 0

Query: 115 DAIDLSSSSSNSKFSSTSI---ARSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGG 174
           DA D+   S  SK SS S+    R+E EILQS NLKSFSF+ELK ATRNFRPDS+LGEGG
Sbjct: 21  DAKDI--GSLGSKASSVSVRPSPRTEGEILQSPNLKSFSFAELKSATRNFRPDSVLGEGG 80

Query: 175 FGSVYKGWIDEHSFSAAKPGSGTVVAVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLVRLI 234
           FG V+KGWIDE S +A++PG+G V+AVK+LN DG QGH+EWLAEVN+LGQ  H HLV+LI
Sbjct: 81  FGCVFKGWIDEKSLTASRPGTGLVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHRHLVKLI 140

Query: 235 GYCLEDEHRLLVYEFLPRGSLENHLFRRGLYFQPLSWSLRLKIALGAAKGLAFLHSDENK 294
           GYCLEDEHRLLVYEF+PRGSLENHLFRRGLYFQPLSW LRLK+ALGAAKGLAFLHS E +
Sbjct: 141 GYCLEDEHRLLVYEFMPRGSLENHLFRRGLYFQPLSWKLRLKVALGAAKGLAFLHSSETR 200

Query: 295 VIYRDFKTSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLS 354
           VIYRDFKTSNILLDS YNAKLSDFGLAKDGP GDKSHVSTR+MGT+GYAAPEYLATGHL+
Sbjct: 201 VIYRDFKTSNILLDSEYNAKLSDFGLAKDGPIGDKSHVSTRVMGTHGYAAPEYLATGHLT 260

Query: 355 AKSDVYSFGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTFRLLDARIERNYS 414
            KSDVYSFGVVLLE+LSGRRA+DKNRP GE+NLVEWAKP+L NKRK FR++D R++  YS
Sbjct: 261 TKSDVYSFGVVLLELLSGRRAVDKNRPSGERNLVEWAKPYLVNKRKIFRVIDNRLQDQYS 320

Query: 415 MESAFRVAILASRCLSAEPKFRPSMSEIVTTLMDLQD-SKGTGATGNRRSRTCKENTD-- 474
           ME A +VA L+ RCL+ E K RP+MSE+V+ L  +Q  +   G   ++  R  +  +D  
Sbjct: 321 MEEACKVATLSLRCLTTEIKLRPNMSEVVSHLEHIQSLNAAIGGNMDKTDRRMRRRSDSV 380

Query: 475 ---KGNA--------HKKVTAYPRPSASALF 489
              K NA           V AYPRPSAS L+
Sbjct: 381 VSKKVNAGFARQTAVGSTVVAYPRPSASPLY 409

BLAST of Spg005756 vs. TAIR 10
Match: AT1G07570.3 (Protein kinase superfamily protein )

HSP 1 Score: 520.8 bits (1340), Expect = 1.2e-147
Identity = 270/391 (69.05%), Postives = 312/391 (79.80%), Query Frame = 0

Query: 115 DAIDLSSSSSNSKFSSTSI---ARSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGG 174
           DA D+   S  SK SS S+    R+E EILQS NLKSFSF+ELK ATRNFRPDS+LGEGG
Sbjct: 35  DAKDI--GSLGSKASSVSVRPSPRTEGEILQSPNLKSFSFAELKSATRNFRPDSVLGEGG 94

Query: 175 FGSVYKGWIDEHSFSAAKPGSGTVVAVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLVRLI 234
           FG V+KGWIDE S +A++PG+G V+AVK+LN DG QGH+EWLAEVN+LGQ  H HLV+LI
Sbjct: 95  FGCVFKGWIDEKSLTASRPGTGLVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHRHLVKLI 154

Query: 235 GYCLEDEHRLLVYEFLPRGSLENHLFRRGLYFQPLSWSLRLKIALGAAKGLAFLHSDENK 294
           GYCLEDEHRLLVYEF+PRGSLENHLFRRGLYFQPLSW LRLK+ALGAAKGLAFLHS E +
Sbjct: 155 GYCLEDEHRLLVYEFMPRGSLENHLFRRGLYFQPLSWKLRLKVALGAAKGLAFLHSSETR 214

Query: 295 VIYRDFKTSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLS 354
           VIYRDFKTSNILLDS YNAKLSDFGLAKDGP GDKSHVSTR+MGT+GYAAPEYLATGHL+
Sbjct: 215 VIYRDFKTSNILLDSEYNAKLSDFGLAKDGPIGDKSHVSTRVMGTHGYAAPEYLATGHLT 274

Query: 355 AKSDVYSFGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTFRLLDARIERNYS 414
            KSDVYSFGVVLLE+LSGRRA+DKNRP GE+NLVEWAKP+L NKRK FR++D R++  YS
Sbjct: 275 TKSDVYSFGVVLLELLSGRRAVDKNRPSGERNLVEWAKPYLVNKRKIFRVIDNRLQDQYS 334

Query: 415 MESAFRVAILASRCLSAEPKFRPSMSEIVTTLMDLQD-SKGTGATGNRRSRTCKENTD-- 474
           ME A +VA L+ RCL+ E K RP+MSE+V+ L  +Q  +   G   ++  R  +  +D  
Sbjct: 335 MEEACKVATLSLRCLTTEIKLRPNMSEVVSHLEHIQSLNAAIGGNMDKTDRRMRRRSDSV 394

Query: 475 ---KGNA--------HKKVTAYPRPSASALF 489
              K NA           V AYPRPSAS L+
Sbjct: 395 VSKKVNAGFARQTAVGSTVVAYPRPSASPLY 423

BLAST of Spg005756 vs. TAIR 10
Match: AT2G28930.2 (protein kinase 1B )

HSP 1 Score: 516.5 bits (1329), Expect = 2.3e-146
Identity = 279/445 (62.70%), Postives = 323/445 (72.58%), Query Frame = 0

Query: 59  MGICFSARIKAVSTCSTGTAIFSCVGNWDILVLLNLSGMDILHSSHAGGNLNSLGRDAID 118
           MGIC SA+IKAVS                                  G +   +  +A D
Sbjct: 1   MGICLSAQIKAVS---------------------------------PGASPKYMSSEAND 60

Query: 119 LSSSSSNSKFSSTSIARSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKG 178
            S  S +S  S  +  R+E EILQS NLKSF+F+ELK ATRNFRPDS+LGEGGFGSV+KG
Sbjct: 61  -SLGSKSSSVSIRTNPRTEGEILQSPNLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKG 120

Query: 179 WIDEHSFSAAKPGSGTVVAVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLVRLIGYCLEDE 238
           WIDE + +A+KPG+G V+AVK+LN DG QGH+EWLAEVN+LGQ  H +LV+LIGYCLEDE
Sbjct: 121 WIDEQTLTASKPGTGVVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHPNLVKLIGYCLEDE 180

Query: 239 HRLLVYEFLPRGSLENHLFRRGLYFQPLSWSLRLKIALGAAKGLAFLHSDENKVIYRDFK 298
           HRLLVYEF+PRGSLENHLFRRG YFQPLSW+LRLK+ALGAAKGLAFLH+ E  VIYRDFK
Sbjct: 181 HRLLVYEFMPRGSLENHLFRRGSYFQPLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFK 240

Query: 299 TSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSAKSDVYS 358
           TSNILLDS YNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHL+ KSDVYS
Sbjct: 241 TSNILLDSEYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDVYS 300

Query: 359 FGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTFRLLDARIERNYSMESAFRV 418
           +GVVLLE+LSGRRA+DKNRPPGEQ LVEWA+P LANKRK FR++D R++  YSME A +V
Sbjct: 301 YGVVLLEVLSGRRAVDKNRPPGEQKLVEWARPLLANKRKLFRVIDNRLQDQYSMEEACKV 360

Query: 419 AILASRCLSAEPKFRPSMSEIVTTLMDLQDSKGTGATG----NRRSR------TCKENTD 478
           A LA RCL+ E K RP+M+E+V+ L  +Q     G        RR R         +  +
Sbjct: 361 ATLALRCLTFEIKLRPNMNEVVSHLEHIQTLNEAGGRNIDMVQRRMRRRSDSVAINQKPN 411

Query: 479 KGNAHKK-----VTAYPRPSASALF 489
            G A +       TAYPRPS S LF
Sbjct: 421 AGFARQTAVGVIATAYPRPSDSPLF 411

BLAST of Spg005756 vs. TAIR 10
Match: AT2G28930.3 (protein kinase 1B )

HSP 1 Score: 516.5 bits (1329), Expect = 2.3e-146
Identity = 279/445 (62.70%), Postives = 323/445 (72.58%), Query Frame = 0

Query: 59  MGICFSARIKAVSTCSTGTAIFSCVGNWDILVLLNLSGMDILHSSHAGGNLNSLGRDAID 118
           MGIC SA+IKAVS                                  G +   +  +A D
Sbjct: 1   MGICLSAQIKAVS------------------------------PGKPGASPKYMSSEAND 60

Query: 119 LSSSSSNSKFSSTSIARSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKG 178
            S  S +S  S  +  R+E EILQS NLKSF+F+ELK ATRNFRPDS+LGEGGFGSV+KG
Sbjct: 61  -SLGSKSSSVSIRTNPRTEGEILQSPNLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKG 120

Query: 179 WIDEHSFSAAKPGSGTVVAVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLVRLIGYCLEDE 238
           WIDE + +A+KPG+G V+AVK+LN DG QGH+EWLAEVN+LGQ  H +LV+LIGYCLEDE
Sbjct: 121 WIDEQTLTASKPGTGVVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHPNLVKLIGYCLEDE 180

Query: 239 HRLLVYEFLPRGSLENHLFRRGLYFQPLSWSLRLKIALGAAKGLAFLHSDENKVIYRDFK 298
           HRLLVYEF+PRGSLENHLFRRG YFQPLSW+LRLK+ALGAAKGLAFLH+ E  VIYRDFK
Sbjct: 181 HRLLVYEFMPRGSLENHLFRRGSYFQPLSWTLRLKVALGAAKGLAFLHNAETSVIYRDFK 240

Query: 299 TSNILLDSNYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSAKSDVYS 358
           TSNILLDS YNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHL+ KSDVYS
Sbjct: 241 TSNILLDSEYNAKLSDFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDVYS 300

Query: 359 FGVVLLEMLSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTFRLLDARIERNYSMESAFRV 418
           +GVVLLE+LSGRRA+DKNRPPGEQ LVEWA+P LANKRK FR++D R++  YSME A +V
Sbjct: 301 YGVVLLEVLSGRRAVDKNRPPGEQKLVEWARPLLANKRKLFRVIDNRLQDQYSMEEACKV 360

Query: 419 AILASRCLSAEPKFRPSMSEIVTTLMDLQDSKGTGATG----NRRSR------TCKENTD 478
           A LA RCL+ E K RP+M+E+V+ L  +Q     G        RR R         +  +
Sbjct: 361 ATLALRCLTFEIKLRPNMNEVVSHLEHIQTLNEAGGRNIDMVQRRMRRRSDSVAINQKPN 414

Query: 479 KGNAHKK-----VTAYPRPSASALF 489
            G A +       TAYPRPS S LF
Sbjct: 421 AGFARQTAVGVIATAYPRPSDSPLF 414

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038894946.11.4e-20687.47probable serine/threonine-protein kinase PBL10 [Benincasa hispida][more]
XP_022970537.11.3e-20482.86probable serine/threonine-protein kinase PBL10 isoform X1 [Cucurbita maxima][more]
KAG7019189.18.5e-20485.61putative serine/threonine-protein kinase PBL10 [Cucurbita argyrosperma subsp. ar... [more]
KAG6583426.18.5e-20485.61putative serine/threonine-protein kinase PBL10, partial [Cucurbita argyrosperma ... [more]
XP_022970539.11.1e-20385.38probable serine/threonine-protein kinase PBL10 isoform X3 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q065481.7e-14669.05Probable serine/threonine-protein kinase PBL9 OS=Arabidopsis thaliana OX=3702 GN... [more]
P465733.2e-14562.70Probable serine/threonine-protein kinase PBL10 OS=Arabidopsis thaliana OX=3702 G... [more]
Q65XV81.6e-14167.12Receptor-like cytoplasmic kinase 176 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
P432935.3e-13264.03Probable serine/threonine-protein kinase PBL11 OS=Arabidopsis thaliana OX=3702 G... [more]
O488143.0e-12755.53Serine/threonine-protein kinase BIK1 OS=Arabidopsis thaliana OX=3702 GN=BIK1 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1HZE06.4e-20582.86probable serine/threonine-protein kinase PBL10 isoform X1 OS=Cucurbita maxima OX... [more]
A0A6J1I5T65.4e-20485.38probable serine/threonine-protein kinase PBL10 isoform X3 OS=Cucurbita maxima OX... [more]
A0A6J1HLY92.7e-20385.38probable serine/threonine-protein kinase PBL10 isoform X2 OS=Cucurbita moschata ... [more]
A0A5A7VI251.7e-20286.54Protein kinase APK1B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold352... [more]
A0A1S3C5673.0e-20286.54protein kinase APK1B, chloroplastic-like isoform X1 OS=Cucumis melo OX=3656 GN=L... [more]
Match NameE-valueIdentityDescription
AT1G07570.21.2e-14769.05Protein kinase superfamily protein [more]
AT1G07570.11.2e-14769.05Protein kinase superfamily protein [more]
AT1G07570.31.2e-14769.05Protein kinase superfamily protein [more]
AT2G28930.22.3e-14662.70protein kinase 1B [more]
AT2G28930.32.3e-14662.70protein kinase 1B [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 165..443
e-value: 5.2E-45
score: 153.8
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 247..470
e-value: 9.4E-61
score: 206.7
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 135..246
e-value: 7.3E-34
score: 118.0
NoneNo IPR availablePIRSRPIRSR037921-1PIRSR037921-1coord: 190..375
e-value: 4.8E-26
score: 89.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 461..475
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 448..475
NoneNo IPR availablePANTHERPTHR45621:SF184KINASE 1Bcoord: 114..475
NoneNo IPR availablePANTHERPTHR45621OS01G0588500 PROTEIN-RELATEDcoord: 114..475
NoneNo IPR availableCDDcd14066STKc_IRAKcoord: 167..446
e-value: 7.94798E-93
score: 281.469
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 167..199
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 292..304
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 161..446
score: 38.901726
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 138..443

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg005756.1Spg005756.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity