Spg005059 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg005059 vs. NCBI nr
Match: XP_038877689.1 (putative disease resistance protein RGA3 [Benincasa hispida])

HSP 1 Score: 542.3 bits (1396), Expect = 4.0e-150
Identity = 297/465 (63.87%), Postives = 350/465 (75.27%), Query Frame = 0

Query: 1   MWIAQGFIQP---REDLIMEDIGEEYFNFLLSRSLFQDVIKDKNGRIIAFKMHDLIHDIA 60
           MWIAQGFIQP   RE+LIMEDIGEEYFNFLLSRSLFQDV KD+NGRI+ FKMHDLIHDIA
Sbjct: 447 MWIAQGFIQPQEERENLIMEDIGEEYFNFLLSRSLFQDVDKDENGRIVTFKMHDLIHDIA 506

Query: 61  CAVSNCKKMDSNPYNLSGRQEK-LRTLIYSNGEFDSKI-VDYTCLRVLVPNTFWTKNLPI 120
           C VSN +K  SNP N SG+  + LR LI +N E   KI  +  CLRVLV N+F T NL I
Sbjct: 507 CMVSNHQKKASNPNNWSGKSARTLRALICNNEETPHKIQANINCLRVLVLNSFETNNLSI 566

Query: 121 LMDKLLHLRYLDISNIRRYGNELILESISMLYNLQTLKLGHMIISLLKNLGKLVNLRHLE 180
           +MDKL+HLRYLDIS      N+L  ES+S LYNLQTLK G++   L KNL KLVNLRHLE
Sbjct: 567 IMDKLIHLRYLDISKCS--VNQLSQESLSSLYNLQTLKFGYIGSDLPKNLRKLVNLRHLE 626

Query: 181 FEGPRVIEKMPSHIGKLIHLQTLYQFAVGFEKGCKIEELGPLKNLKGTLMLTNLENVKSK 240
           F+    +++M SH+G LIHLQTL +F VG  K  KIEELGPLKNLKGTL++TNLE V++K
Sbjct: 627 FQKKFDMKQMASHMGNLIHLQTLSEFVVGLGKAYKIEELGPLKNLKGTLIITNLEKVQNK 686

Query: 241 EEAISAKLVEKKNLCELNFCW--NFCCEGEYEKCNNLEVLEGLQPHKNLQSLGILYFGGE 300
           EEAI+AKLVEKKNL  L   W       GEY + +++ VLEGLQPHKNLQSL I  F G+
Sbjct: 687 EEAIAAKLVEKKNLRHLILKWFVGLYNRGEYYEDDHVRVLEGLQPHKNLQSLEINGFKGK 746

Query: 301 VLPNGVFVENLVKIKLRSCEKCKVLPMLGQLPNQKKLDIDSMDGVRSIGNEFYGIDSNQ- 360
           V PN +FV+NLV++ L  CE+C +LPMLGQLPN KKL I SM  V+SIGNEFYGIDSNQ 
Sbjct: 747 VFPNNIFVQNLVEVDLYKCERCVMLPMLGQLPNLKKLLIRSMVSVKSIGNEFYGIDSNQL 806

Query: 361 --THSFPQLKKLCIDGMKNLEQWGEATLV-PNLFGSLQQVEIYGCEKLTKLPSGIECCSS 420
             + +FPQLK+L I GMK LEQW E+T+V  NLFG L+++ I GC +L KLPSG+E C S
Sbjct: 807 RNSFAFPQLKELHIYGMKKLEQWDESTVVSSNLFGCLKKLHISGCGQLAKLPSGLEGCHS 866

Query: 421 IKYLTIRDCPNLMLNVQNLQNLYHLDMNGLERLPKGLAHTMRYQI 455
           I+YL I  C NLMLNVQN  NLYHLD+NGL+RLP+GL      +I
Sbjct: 867 IEYLGIFQCSNLMLNVQNFYNLYHLDINGLKRLPEGLGKLTNLKI 909

BLAST of Spg005059 vs. NCBI nr
Match: XP_038877687.1 (putative disease resistance protein RGA1 [Benincasa hispida] >XP_038877688.1 putative disease resistance protein RGA1 [Benincasa hispida])

HSP 1 Score: 529.3 bits (1362), Expect = 3.5e-146
Identity = 284/435 (65.29%), Postives = 336/435 (77.24%), Query Frame = 0

Query: 1   MWIAQGFI---QPREDLIMEDIGEEYFNFLLSRSLFQDVIKDKNGRIIAFKMHDLIHDIA 60
           MWIAQGFI   Q RE+L+MEDIGEEYFNFLLSRSLFQDVI+D+N RII FKMHDL+HDIA
Sbjct: 452 MWIAQGFIQSQQERENLVMEDIGEEYFNFLLSRSLFQDVIRDENKRIITFKMHDLMHDIA 511

Query: 61  CAVSNCKKMDSNPYNLSGRQE-KLRTLIYSNGEFDSKIVD-YTCLRVLVPNTFWTKNLPI 120
           CA+SN + M SNP NLSGR   KLRTL+Y + E   K+ D +  LRVL  +  W  NLP 
Sbjct: 512 CAISNNQNMVSNPNNLSGRSAGKLRTLVYRDEENQYKVCDNFFRLRVLKQDKIWANNLPY 571

Query: 121 LMDKLLHLRYLDISNIRRYGNELILESISMLYNLQTLKLGHMIISLLKNLGKLVNLRHLE 180
           L+DKL+HLRYLD+S  + Y  + + ES+ +LYNLQTLKLG++   L +NL KLVNLRHLE
Sbjct: 572 LLDKLIHLRYLDLS--KCYIIKNLRESLCLLYNLQTLKLGYIESELPENLRKLVNLRHLE 631

Query: 181 FEGPRVIEKMPSHIGKLIHLQTLYQFAVGFEKGCKIEELGPLKNLKGTLMLTNLENVKSK 240
           F     +++MPSH+G LIHLQTL +FAVGFEKGCKIEELG LKNLK  L+LTNLE +KSK
Sbjct: 632 FYVVPGMKQMPSHMGNLIHLQTLSRFAVGFEKGCKIEELGTLKNLKDELILTNLEKLKSK 691

Query: 241 EEAISAKLVEKKNLCELNFCWNFCCEGEYEKCNNLEVLEGLQPHKNLQSLGILYFGGEVL 300
           EEA++AKLVEKK L  L F W      EY+K N ++VLEGLQPHKNLQSL I  FGGEVL
Sbjct: 692 EEAMAAKLVEKKKLRRLLFRWTLFYGVEYDKHNFVQVLEGLQPHKNLQSLNISGFGGEVL 751

Query: 301 PNGVFVENLVKIKLRSCEKCKVLPMLGQLPNQKKLDIDSMDGVRSIGNEFYGIDSN---Q 360
           PN +FVENLV+I+L  C KC+VLPMLGQL N KKL+I  M+ VRSIGNEFYGID N    
Sbjct: 752 PNDIFVENLVEIRLFDCSKCEVLPMLGQLANLKKLEITWMNNVRSIGNEFYGIDPNLQRN 811

Query: 361 THSFPQLKKLCIDGMKNLEQWGEATLVPNLFGSLQQVEIYGCEKLTKLPSGIECCSSIKY 420
           + +FPQL++L IDGMK LEQW E T   NLFGSL+++ + GC+KLTKLPSG E C SI+Y
Sbjct: 812 SFAFPQLEELYIDGMKKLEQWDEFT-TSNLFGSLKKLSVLGCDKLTKLPSGFEGCHSIEY 871

Query: 421 LTIRDCPNLMLNVQN 428
           + I  CPNLMLNVQN
Sbjct: 872 V-IERCPNLMLNVQN 882

BLAST of Spg005059 vs. NCBI nr
Match: XP_031741283.1 (disease resistance protein RGA2-like [Cucumis sativus])

HSP 1 Score: 520.0 bits (1338), Expect = 2.1e-143
Identity = 281/453 (62.03%), Postives = 341/453 (75.28%), Query Frame = 0

Query: 1   MWIAQGFIQP---REDLIMEDIGEEYFNFLLSRSLFQDVIKDKNGRIIAFKMHDLIHDIA 60
           MWIA+GFIQP   RE+L ME+IGEEY NFLLSRSLF+D IK  +GRI+ FK+HDL+HDIA
Sbjct: 330 MWIAEGFIQPEQERENLTMENIGEEYLNFLLSRSLFEDAIK-YDGRIVTFKIHDLMHDIA 389

Query: 61  CAVSNCKKMDSNPYNLSGRQ-EKLRTLIYSNGEFDSKI-VDYTCLRVLVPNTFWTKNLPI 120
           CA+SN  KMDSNP + +G+   KLRTLI  N E   KI  D  CLRVLV   F T  L  
Sbjct: 390 CAISNHHKMDSNPISWNGKSTRKLRTLICENEEAFHKIQTDIICLRVLVLKWFDTNTLST 449

Query: 121 LMDKLLHLRYLDISNIRRYGNELILESISMLYNLQTLKLGHMIISLLKNLGKLVNLRHLE 180
           +MDKL+HLRYLDISN     N+L+ +SI  LYNLQTLKLG++   L KNL  LVNLRHLE
Sbjct: 450 IMDKLIHLRYLDISNCNI--NKLLRDSICALYNLQTLKLGYIECDLPKNLRNLVNLRHLE 509

Query: 181 FEGPRVIEKMPSHIGKLIHLQTLYQFAVGFEKGCKIEELGPLKNLKGTLMLTNLENVKSK 240
           F+    + +MPSH+G +IHLQTL +F VG EKGCKI+ELGPLK+LKGTL L NL+NV++K
Sbjct: 510 FKKFFDMGQMPSHMGNMIHLQTLSEFVVGLEKGCKIDELGPLKDLKGTLTLKNLQNVQNK 569

Query: 241 EEAISAKLVEKKNLCELNFCW--NFCCEGEYEKCNNLEVLEGLQPHKNLQSLGILYFGGE 300
           +EA++AKLVEKK L  L F W  N    GEY++ +N +VLEGLQPHKN+QSL I  F G 
Sbjct: 570 DEAMAAKLVEKKYLRHLIFQWFLNLYDRGEYDEDDNKQVLEGLQPHKNVQSLDIRGFQGR 629

Query: 301 VLPNGVFVENLVKIKLRSCEKCKVLPMLGQLPNQKKLDIDSMDGVRSIGNEFYGIDSNQT 360
           VL N +FVENLV+I+L  C +C+VLPMLGQLPN KKL+I SM+ VRSIG+EFYG+D N  
Sbjct: 630 VLNNNIFVENLVEIRLVDCGRCEVLPMLGQLPNLKKLEIISMNSVRSIGSEFYGVDCNDR 689

Query: 361 HS--FPQLKKLCIDGMKNLEQWGEATL-VPNLFGSLQQVEIYGCEKLTKLPSGIECCSSI 420
           +S  FPQL K  I G+K L+QW EAT+   N FG L+++ + GC +L KLPSG+E C SI
Sbjct: 690 NSSAFPQLNKFHICGLKKLQQWDEATVFASNRFGCLKELILSGCHQLAKLPSGLEGCYSI 749

Query: 421 KYLTIRDCPNLMLNVQNLQNLYHLDMNGLERLP 444
           +YL I  CPNLMLNVQNL NLYHLD+ GL+RLP
Sbjct: 750 EYLAIDGCPNLMLNVQNLYNLYHLDIRGLKRLP 779

BLAST of Spg005059 vs. NCBI nr
Match: XP_004140442.1 (disease resistance protein RGA2 [Cucumis sativus] >KGN50811.1 hypothetical protein Csa_018865 [Cucumis sativus])

HSP 1 Score: 520.0 bits (1338), Expect = 2.1e-143
Identity = 281/453 (62.03%), Postives = 341/453 (75.28%), Query Frame = 0

Query: 1   MWIAQGFIQP---REDLIMEDIGEEYFNFLLSRSLFQDVIKDKNGRIIAFKMHDLIHDIA 60
           MWIA+GFIQP   RE+L ME+IGEEY NFLLSRSLF+D IK  +GRI+ FK+HDL+HDIA
Sbjct: 447 MWIAEGFIQPEQERENLTMENIGEEYLNFLLSRSLFEDAIK-YDGRIVTFKIHDLMHDIA 506

Query: 61  CAVSNCKKMDSNPYNLSGRQ-EKLRTLIYSNGEFDSKI-VDYTCLRVLVPNTFWTKNLPI 120
           CA+SN  KMDSNP + +G+   KLRTLI  N E   KI  D  CLRVLV   F T  L  
Sbjct: 507 CAISNHHKMDSNPISWNGKSTRKLRTLICENEEAFHKIQTDIICLRVLVLKWFDTNTLST 566

Query: 121 LMDKLLHLRYLDISNIRRYGNELILESISMLYNLQTLKLGHMIISLLKNLGKLVNLRHLE 180
           +MDKL+HLRYLDISN     N+L+ +SI  LYNLQTLKLG++   L KNL  LVNLRHLE
Sbjct: 567 IMDKLIHLRYLDISNCNI--NKLLRDSICALYNLQTLKLGYIECDLPKNLRNLVNLRHLE 626

Query: 181 FEGPRVIEKMPSHIGKLIHLQTLYQFAVGFEKGCKIEELGPLKNLKGTLMLTNLENVKSK 240
           F+    + +MPSH+G +IHLQTL +F VG EKGCKI+ELGPLK+LKGTL L NL+NV++K
Sbjct: 627 FKKFFDMGQMPSHMGNMIHLQTLSEFVVGLEKGCKIDELGPLKDLKGTLTLKNLQNVQNK 686

Query: 241 EEAISAKLVEKKNLCELNFCW--NFCCEGEYEKCNNLEVLEGLQPHKNLQSLGILYFGGE 300
           +EA++AKLVEKK L  L F W  N    GEY++ +N +VLEGLQPHKN+QSL I  F G 
Sbjct: 687 DEAMAAKLVEKKYLRHLIFQWFLNLYDRGEYDEDDNKQVLEGLQPHKNVQSLDIRGFQGR 746

Query: 301 VLPNGVFVENLVKIKLRSCEKCKVLPMLGQLPNQKKLDIDSMDGVRSIGNEFYGIDSNQT 360
           VL N +FVENLV+I+L  C +C+VLPMLGQLPN KKL+I SM+ VRSIG+EFYG+D N  
Sbjct: 747 VLNNNIFVENLVEIRLVDCGRCEVLPMLGQLPNLKKLEIISMNSVRSIGSEFYGVDCNDR 806

Query: 361 HS--FPQLKKLCIDGMKNLEQWGEATL-VPNLFGSLQQVEIYGCEKLTKLPSGIECCSSI 420
           +S  FPQL K  I G+K L+QW EAT+   N FG L+++ + GC +L KLPSG+E C SI
Sbjct: 807 NSSAFPQLNKFHICGLKKLQQWDEATVFASNRFGCLKELILSGCHQLAKLPSGLEGCYSI 866

Query: 421 KYLTIRDCPNLMLNVQNLQNLYHLDMNGLERLP 444
           +YL I  CPNLMLNVQNL NLYHLD+ GL+RLP
Sbjct: 867 EYLAIDGCPNLMLNVQNLYNLYHLDIRGLKRLP 896

BLAST of Spg005059 vs. NCBI nr
Match: XP_004136783.2 (disease resistance protein RGA2 [Cucumis sativus])

HSP 1 Score: 505.0 bits (1299), Expect = 7.0e-139
Identity = 287/461 (62.26%), Postives = 346/461 (75.05%), Query Frame = 0

Query: 1   MWIAQGFIQPRE----DLIMEDIGEEYFNFLLSRSLFQDVIKDKNGRIIAFKMHDLIHDI 60
           MWIAQGFIQP +    +L+MEDIGE+YFNFLLSRS+FQDV +D N RI+ FKMHDL+HDI
Sbjct: 454 MWIAQGFIQPGQGRDKNLLMEDIGEQYFNFLLSRSIFQDVTRDANKRIVGFKMHDLMHDI 513

Query: 61  ACAVSNCKKMDSNPYNLSGRQ-EKLRTLIYSNGEFDSKI--VDYTCLRVLVPNTFWTKNL 120
           ACA+S+ + ++SNP NLSG+   KLRTLI  N E  + +   D  CLRVL        +L
Sbjct: 514 ACAISSHQNVESNPNNLSGKSVRKLRTLI-CNDEVINYLNQNDIVCLRVLKVIFQSHTDL 573

Query: 121 PILMDKLLHLRYLDISNIRRYGNELILESISMLYNLQTLKLGHMIISLLKNLGKLVNLRH 180
            I +DKL+HLRYLDIS      N+L+LES+S+LYNLQTLKLG     L KNL KLVNLRH
Sbjct: 574 WIPIDKLIHLRYLDISECSI--NKLLLESLSLLYNLQTLKLGQS--GLPKNLRKLVNLRH 633

Query: 181 LEFE--GPRVIEKMPSHIGKLIHLQTLYQFAVGFEKGCKIEELGPLKNLKGTLMLTNLEN 240
           LEF+  G      MPS +G LIHLQ+L  F VGFEKGCKIEELGPLKNLKG L LTNL  
Sbjct: 634 LEFKMFGDTA---MPSDMGNLIHLQSLSGFLVGFEKGCKIEELGPLKNLKGKLTLTNLWR 693

Query: 241 VKSKEEAISAKLVEKKNLCELNFCWNFCCE--GEYEKCNNLEVLEGLQPHKNLQSLGILY 300
           V++K+EA++AKLVEKKNL  LN  W F  +  GE ++   ++VLEGLQPHKNLQSL IL 
Sbjct: 694 VQNKDEAMAAKLVEKKNLRHLNL-WFFETDKRGEDDEDGIVQVLEGLQPHKNLQSLEILG 753

Query: 301 FGGEVLPNGVFVENLVKIKLRSCEKCKVLPMLGQLPNQKKLDIDSMDGVRSIGNEFYGID 360
           F G+VLP G+FVENLVKI+L   E+C+VLPMLGQLPN K+L+I  M+ VRSIGNEFYG+D
Sbjct: 754 FRGKVLPTGIFVENLVKIRLGHFERCEVLPMLGQLPNLKELEIMYMESVRSIGNEFYGVD 813

Query: 361 SNQTHS--FPQLKKLCIDGMKNLEQWGEATLV--PNLFGSLQQVEIYGCEKLTKLPSGIE 420
           S+  +S  FPQLKKL I  M NLEQW EAT+V   NLFG L++V I  C  L KLPSG+E
Sbjct: 814 SSHQNSVAFPQLKKLSIYEMMNLEQWDEATVVLESNLFGCLKEVRIRRCNPLAKLPSGLE 873

Query: 421 CCSSIKYLTIRDCPNLMLNVQNLQNLYHLDMNGLERLPKGL 447
            C S++YL+IR C NLMLNVQNL  LYHL+++GL+RLPKG+
Sbjct: 874 GCHSLEYLSIRGCFNLMLNVQNLHKLYHLEIDGLKRLPKGM 905

BLAST of Spg005059 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 177.6 bits (449), Expect = 3.4e-43
Identity = 156/481 (32.43%), Postives = 232/481 (48.23%), Query Frame = 0

Query: 2   WIAQGFIQPREDLIMEDIGEEYFNFLLSRSLFQDVIKDKNGRIIAFKMHDLIHDIACAVS 61
           W+A GF+  + +L +ED+G E +N L  RS FQ+ I+ ++G+   FKMHDLIHD+A ++ 
Sbjct: 427 WMAHGFLLSKGNLELEDVGNEVWNELYLRSFFQE-IEVESGKTY-FKMHDLIHDLATSLF 486

Query: 62  NCKKMDSN----PYNLSGRQEKLRTLIYSNGEFDSKIVDYTCLRVLVPNTFWTKNLPILM 121
           +     SN      N  G    +      +    S +  +  LRVL         LP  +
Sbjct: 487 SANTSSSNIREINANYDGYMMSIGFAEVVSSYSPSLLQKFVSLRVLNLRNSNLNQLPSSI 546

Query: 122 DKLLHLRYLDISNIRRYGNELILESISMLYNLQTLKLGH--MIISLLKNLGKLVNLRHLE 181
             L+HLRYLD+S   R  N  + + +  L NLQTL L +   +  L K   KL +LR+L 
Sbjct: 547 GDLVHLRYLDLSGNFRIRN--LPKRLCKLQNLQTLDLHYCDSLSCLPKQTSKLGSLRNLL 606

Query: 182 FEGPRVIEKMPSHIGKLIHLQTLYQFAVGFEKGCKIEELGPLKNLKGTLMLTNLENVKSK 241
            +G   +   P  IG L  L++L  F +G  KG ++ EL  L NL G++ +T L+ VK  
Sbjct: 607 LDGCS-LTSTPPRIGLLTCLKSLSCFVIGKRKGHQLGELKNL-NLYGSISITKLDRVKKD 666

Query: 242 EEAISAKLVEKKNLCELNFCWNFCCEGEYEKCNNLEVLEGLQPHKNLQSLGILYFGGEVL 301
            +A  A L  K NL  L   W+   +  Y+     EVLE L+PH NL+ L I  FGG  L
Sbjct: 667 TDAKEANLSAKANLHSLCLSWDLDGKHRYDS----EVLEALKPHSNLKYLEINGFGGIRL 726

Query: 302 P---NGVFVENLVKIKLRSCEKCKVLPMLGQLPNQKKLDIDSMDGVRSIGNEFYGIDSNQ 361
           P   N   ++N+V I++R CE C  LP  G+LP  + L++ +        +  Y  D+  
Sbjct: 727 PDWMNQSVLKNVVSIRIRGCENCSCLPPFGELPCLESLELHT-----GSADVEYVEDNVH 786

Query: 362 THSFPQLKKLCIDGMKN----LEQWGEATLVPNLFGSLQQVEIYGCEKLTKLPSGIECCS 421
              FP L+KL I    N    L+  GE       F  L+++  Y C         I   S
Sbjct: 787 PGRFPSLRKLVIWDFSNLKGLLKMEGE-----KQFPVLEEMTFYWCPMFV-----IPTLS 846

Query: 422 SIKYL-TIRDCPNLMLNVQNLQNLYHLDMNG-------LERLPKGLAHTMRYQIKLSRRL 462
           S+K L  I     ++ ++ NL+ L  LD++         E + K LA+    +I   R L
Sbjct: 847 SVKTLKVIVTDATVLRSISNLRALTSLDISDNVEATSLPEEMFKSLANLKYLKISFFRNL 882

BLAST of Spg005059 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 5.7e-43
Identity = 155/472 (32.84%), Postives = 239/472 (50.64%), Query Frame = 0

Query: 1   MWIAQGFIQPREDLIMEDIGEEYFNFLLSRSLFQDVIKDKNGRIIAFKMHDLIHDIACAV 60
           +W+A GF+  + ++ +ED+G+E +  L  RS FQ+ I+ K+G+   FKMHDLIHD+A ++
Sbjct: 427 LWMAHGFLLSKGNMELEDVGDEVWKELYLRSFFQE-IEVKDGKTY-FKMHDLIHDLATSL 486

Query: 61  SNCKKMDSNPYNLSGRQEKLRTLIYSNGEFDSKIVDYT--------CLRVLVPNTFWTKN 120
            +     SN   ++   +   T + S G F   +  YT         LRVL         
Sbjct: 487 FSANTSSSNIREIN---KHSYTHMMSIG-FAEVVFFYTLPPLEKFISLRVLNLGDSTFNK 546

Query: 121 LPILMDKLLHLRYLDISNIRRYGNELILESISMLYNLQTLKLGH--MIISLLKNLGKLVN 180
           LP  +  L+HLRYL   N+   G   + + +  L NLQTL L +   +  L K   KL +
Sbjct: 547 LPSSIGDLVHLRYL---NLYGSGMRSLPKQLCKLQNLQTLDLQYCTKLCCLPKETSKLGS 606

Query: 181 LRHLEFEGPRVIEKMPSHIGKLIHLQTLYQFAVGFEKGCKIEELGPLKNLKGTLMLTNLE 240
           LR+L  +G + +  MP  IG L  L+TL QF VG +KG ++ ELG L NL G++ +++LE
Sbjct: 607 LRNLLLDGSQSLTCMPPRIGSLTCLKTLGQFVVGRKKGYQLGELGNL-NLYGSIKISHLE 666

Query: 241 NVKSKEEAISAKLVEKKNLCELNFCWNFCCEGEYEKCNNLEVLEGLQPHKNLQSLGILYF 300
            VK+ ++A  A L  K NL  L+  WN      YE    ++VLE L+PH NL SL I  F
Sbjct: 667 RVKNDKDAKEANLSAKGNLHSLSMSWNNFGPHIYES-EEVKVLEALKPHSNLTSLKIYGF 726

Query: 301 GGEVLP---NGVFVENLVKIKLRSCEKCKVLPMLGQLPNQKKLDID----SMDGVRSIGN 360
            G  LP   N   ++N+V I + +   C  LP  G LP  + L++      ++ V  +  
Sbjct: 727 RGIHLPEWMNHSVLKNIVSILISNFRNCSCLPPFGDLPCLESLELHWGSADVEYVEEVDI 786

Query: 361 EFYGIDSNQTHSFPQLKKLCI---DGMKN-LEQWGEATLVPNLFGSLQQVEIYGCEKLTK 420
           + +     +   FP L+KL I     +K  L++ GE       F  L+++ I+ C  LT 
Sbjct: 787 DVHSGFPTRIR-FPSLRKLDIWDFGSLKGLLKKEGE-----EQFPVLEEMIIHECPFLT- 846

Query: 421 LPSGIECCSSIKYLTIRDCPN----LMLNVQNLQNLYHLDMNGLERLPKGLA 448
           L S +   +S++    +   +    +  N+ NL+ L     N L+ LP  LA
Sbjct: 847 LSSNLRALTSLRICYNKVATSFPEEMFKNLANLKYLTISRCNNLKELPTSLA 880

BLAST of Spg005059 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 176.0 bits (445), Expect = 9.8e-43
Identity = 156/485 (32.16%), Postives = 245/485 (50.52%), Query Frame = 0

Query: 1   MWIAQGFIQPREDLIMEDIGEEYFNFLLSRSLFQDVIKDKNGRIIAFKMHDLIHDIACAV 60
           +W+A  F+  + ++ +ED+G E +N L  RS FQ+ I+ K+G+   FKMHDLIHD+A ++
Sbjct: 426 LWMAHSFLLSKGNMELEDVGNEVWNELYLRSFFQE-IEVKSGKTY-FKMHDLIHDLATSM 485

Query: 61  SNCKKMDSNPYNLSGRQEKLRTLIYSNGE------FDSKIVDYT--------CLRVLVPN 120
            +      +   ++ + ++    I +N +      F   +  Y+         LRVL  +
Sbjct: 486 FSASASSRSIRQINVKDDEDMMFIVTNYKDMMSIGFSEVVSSYSPSLFKRFVSLRVLNLS 545

Query: 121 TFWTKNLPILMDKLLHLRYLDISNIRRYGNEL--ILESISMLYNLQTLKLGH--MIISLL 180
               + LP  +  L+HLRYLD+S     GN++  + + +  L NLQTL L +   +  L 
Sbjct: 546 NSEFEQLPSSVGDLVHLRYLDLS-----GNKICSLPKRLCKLQNLQTLDLYNCQSLSCLP 605

Query: 181 KNLGKLVNLRHLEFEGPRVIEKMPSHIGKLIHLQTLYQFAVGFEKGCKIEELGPLKNLKG 240
           K   KL +LR+L  +    +  MP  IG L  L+TL  F VG  KG ++ EL  L NL+G
Sbjct: 606 KQTSKLCSLRNLVLD-HCPLTSMPPRIGLLTCLKTLGYFVVGERKGYQLGELRNL-NLRG 665

Query: 241 TLMLTNLENVKSKEEAISAKLVEKKNLCELNFCWNFCCEGEYEKCNNLEVLEGLQPHKNL 300
            + +T+LE VK+  EA  A L  K NL  L+  W+      YE    ++VLE L+PH NL
Sbjct: 666 AISITHLERVKNDMEAKEANLSAKANLHSLSMSWD--RPNRYES-EEVKVLEALKPHPNL 725

Query: 301 QSLGILYFGGEVLP---NGVFVENLVKIKLRSCEKCKVLPMLGQLPNQKKLDIDSMDGVR 360
           + L I+ F G  LP   N   ++N+V I +  CE C  LP  G+LP  + L++   DG  
Sbjct: 726 KYLEIIDFCGFCLPDWMNHSVLKNVVSILISGCENCSCLPPFGELPCLESLEL--QDG-- 785

Query: 361 SIGNEFYGIDSNQT-HSFPQLKKLCIDG---MKNLEQWGEATLVPNL------------- 420
           S+  E+       T   FP L+KL I G   +K L++   A   P L             
Sbjct: 786 SVEVEYVEDSGFLTRRRFPSLRKLHIGGFCNLKGLQRMKGAEQFPVLEEMKISDCPMFVF 845

Query: 421 --FGSLQQVEIYGCEKLTKLP--SGIECCSSIKYLTIRDCPNLMLNV-QNLQNLYHLDMN 443
               S++++EI+G      L   S +   +S+K  +     +L+  + +NL+NL +L ++
Sbjct: 846 PTLSSVKKLEIWGEADAGGLSSISNLSTLTSLKIFSNHTVTSLLEEMFKNLENLIYLSVS 894

BLAST of Spg005059 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 1.4e-41
Identity = 149/452 (32.96%), Postives = 229/452 (50.66%), Query Frame = 0

Query: 1   MWIAQGFIQPREDLIMEDIGEEYFNFLLSRSLFQDVIKDKNGRIIAFKMHDLIHDIACAV 60
           +W+A GF+  + +L +ED+G E +N L  RS FQ+ I+ K+G    FK+HDLIHD+A ++
Sbjct: 428 LWMAHGFLLSKGNLELEDVGNEVWNELYLRSFFQE-IEAKSGNTY-FKIHDLIHDLATSL 487

Query: 61  SNCKKMDSNPYNLSGRQEKLRTLI----YSNGEFDSKIVDYTCLRVLVPNTFWTKNLPIL 120
            +      N   ++ +  K    I      +    S +  +  LRVL  +    + LP  
Sbjct: 488 FSASASCGNIREINVKDYKHTVSIGFAAVVSSYSPSLLKKFVSLRVLNLSYSKLEQLPSS 547

Query: 121 MDKLLHLRYLDIS--NIRRYGNELILESISMLYNLQTLKLG--HMIISLLKNLGKLVNLR 180
           +  LLHLRYLD+S  N R      + E +  L NLQTL +   + +  L K   KL +LR
Sbjct: 548 IGDLLHLRYLDLSCNNFRS-----LPERLCKLQNLQTLDVHNCYSLNCLPKQTSKLSSLR 607

Query: 181 HLEFEGPRVIEKMPSHIGKLIHLQTLYQFAVGFEKGCKIEELGPLKNLKGTLMLTNLENV 240
           HL  +G   +   P  IG L  L+TL  F VG +KG ++ EL  L NL G++ +T+LE V
Sbjct: 608 HLVVDG-CPLTSTPPRIGLLTCLKTLGFFIVGSKKGYQLGELKNL-NLCGSISITHLERV 667

Query: 241 KSKEEAISAKLVEKKNLCELNFCWNFCCEGEYEKCNNLEVLEGLQPHKNLQSLGILYFGG 300
           K+  +A  A L  K NL  L+  W+      YE    ++VLE L+PH NL+ L I+ FGG
Sbjct: 668 KNDTDA-EANLSAKANLQSLSMSWDNDGPNRYES-KEVKVLEALKPHPNLKYLEIIAFGG 727

Query: 301 EVLP---NGVFVENLVKIKLRSCEKCKVLPMLGQLPNQKKLDIDSMDG-VRSIGNEFYGI 360
              P   N   +E ++ ++++SC+ C  LP  G+LP  + L++ +    V  +  +    
Sbjct: 728 FRFPSWINHSVLEKVISVRIKSCKNCLCLPPFGELPCLENLELQNGSAEVEYVEEDDVHS 787

Query: 361 DSNQTHSFPQLKKLCIDGMKNL----EQWGEATLVPNLFGSLQQVEIYGCEKLTKLPSGI 420
             +   SFP LKKL I   ++L    ++ GE       F  L+++ I  C  L   P+  
Sbjct: 788 RFSTRRSFPSLKKLRIWFFRSLKGLMKEEGE-----EKFPMLEEMAILYC-PLFVFPT-- 847

Query: 421 ECCSSIKYLTIRDCPNL--MLNVQNLQNLYHL 435
              SS+K L +    N   + ++ NL  L  L
Sbjct: 848 --LSSVKKLEVHGNTNTRGLSSISNLSTLTSL 858

BLAST of Spg005059 vs. ExPASy Swiss-Prot
Match: Q9LRR5 (Putative disease resistance protein At3g14460 OS=Arabidopsis thaliana OX=3702 GN=At3g14460 PE=3 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 2.9e-39
Identity = 146/505 (28.91%), Postives = 240/505 (47.52%), Query Frame = 0

Query: 1   MWIAQGFI-QPREDLIMEDIGEEYFNFLLSRSLFQDVIKDKNGRIIAFKMHDLIHDIACA 60
           +W+A   + QPR    +EDIG +Y   L+++S FQ +    +  + +F MHDL++D+A A
Sbjct: 442 LWMAIDLLYQPRSSRRLEDIGNDYLGDLVAQSFFQRL----DITMTSFVMHDLMNDLAKA 501

Query: 61  VSN--CKKMD----------SNPYNLSGRQ-------------EKLRTLIYSNG------ 120
           VS   C +++          +  ++ S  Q             E LRT++  N       
Sbjct: 502 VSGDFCFRLEDDNIPEIPSTTRHFSFSRSQCDASVAFRSICGAEFLRTILPFNSPTSLES 561

Query: 121 -EFDSKIVD-----YTCLRVLVPNTFWTKNLPILMDKLLHLRYLDISNIRRYGNELILES 180
            +   K+++      + LR+L  + +   NLP  +  L  LRYLD+S+ +    + + E 
Sbjct: 562 LQLTEKVLNPLLNALSGLRILSLSHYQITNLPKSLKGLKLLRYLDLSSTK---IKELPEF 621

Query: 181 ISMLYNLQTLKLGHM--IISLLKNLGKLVNLRHLEFEGPRVIEKMPSHIGKLIHLQTLYQ 240
           +  L NLQTL L +   + SL K++ +L+NLR L+  G  ++E MP  I KL  LQ L  
Sbjct: 622 VCTLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLVGTPLVE-MPPGIKKLRSLQKLSN 681

Query: 241 FAVGFEKGCKIEELGPLKNLKGTLMLTNLENVKSKEEAISAKLVEKKNLCELNFCWNFCC 300
           F +G   G  + EL  L +L+GTL ++ L+NV    EA  A L  K  L  L   W    
Sbjct: 682 FVIGRLSGAGLHELKELSHLRGTLRISELQNVAFASEAKDAGLKRKPFLDGLILKWTVKG 741

Query: 301 EG------EYEKCNNLEVLEGLQPHKNLQSLGILYFGGEVLPNGVFVEN---LVKIKLRS 360
            G          C+  EVL  L+PH +L++  I  + G   P  +   +   +  + L S
Sbjct: 742 SGFVPGSFNALACDQKEVLRMLEPHPHLKTFCIESYQGGAFPKWLGDSSFFGITSVTLSS 801

Query: 361 CEKCKVLPMLGQLPNQKKLDIDSMDGVRSIGNE-FYGIDSNQTHSFPQLKKLCIDGMKNL 420
           C  C  LP +GQLP+ K L I+  + ++ +G + F+G ++++   F  L+ L   GM   
Sbjct: 802 CNLCISLPPVGQLPSLKYLSIEKFNILQKVGLDFFFGENNSRGVPFQSLQILKFYGMPRW 861

Query: 421 EQWGEATLVPNLFGSLQQVEIYGCEKL-TKLPSGIECCSSIKYLTIRDCPNLMLNVQNLQ 455
           ++W    L   +F  LQ++ I  C  L  K P G+   + +   TI DCP  +  V   +
Sbjct: 862 DEWICPELEDGIFPCLQKLIIQRCPSLRKKFPEGLPSSTEV---TISDCP--LRAVSGGE 921

BLAST of Spg005059 vs. ExPASy TrEMBL
Match: A0A0A0KST5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G266890 PE=4 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 1.0e-143
Identity = 281/453 (62.03%), Postives = 341/453 (75.28%), Query Frame = 0

Query: 1   MWIAQGFIQP---REDLIMEDIGEEYFNFLLSRSLFQDVIKDKNGRIIAFKMHDLIHDIA 60
           MWIA+GFIQP   RE+L ME+IGEEY NFLLSRSLF+D IK  +GRI+ FK+HDL+HDIA
Sbjct: 447 MWIAEGFIQPEQERENLTMENIGEEYLNFLLSRSLFEDAIK-YDGRIVTFKIHDLMHDIA 506

Query: 61  CAVSNCKKMDSNPYNLSGRQ-EKLRTLIYSNGEFDSKI-VDYTCLRVLVPNTFWTKNLPI 120
           CA+SN  KMDSNP + +G+   KLRTLI  N E   KI  D  CLRVLV   F T  L  
Sbjct: 507 CAISNHHKMDSNPISWNGKSTRKLRTLICENEEAFHKIQTDIICLRVLVLKWFDTNTLST 566

Query: 121 LMDKLLHLRYLDISNIRRYGNELILESISMLYNLQTLKLGHMIISLLKNLGKLVNLRHLE 180
           +MDKL+HLRYLDISN     N+L+ +SI  LYNLQTLKLG++   L KNL  LVNLRHLE
Sbjct: 567 IMDKLIHLRYLDISNCNI--NKLLRDSICALYNLQTLKLGYIECDLPKNLRNLVNLRHLE 626

Query: 181 FEGPRVIEKMPSHIGKLIHLQTLYQFAVGFEKGCKIEELGPLKNLKGTLMLTNLENVKSK 240
           F+    + +MPSH+G +IHLQTL +F VG EKGCKI+ELGPLK+LKGTL L NL+NV++K
Sbjct: 627 FKKFFDMGQMPSHMGNMIHLQTLSEFVVGLEKGCKIDELGPLKDLKGTLTLKNLQNVQNK 686

Query: 241 EEAISAKLVEKKNLCELNFCW--NFCCEGEYEKCNNLEVLEGLQPHKNLQSLGILYFGGE 300
           +EA++AKLVEKK L  L F W  N    GEY++ +N +VLEGLQPHKN+QSL I  F G 
Sbjct: 687 DEAMAAKLVEKKYLRHLIFQWFLNLYDRGEYDEDDNKQVLEGLQPHKNVQSLDIRGFQGR 746

Query: 301 VLPNGVFVENLVKIKLRSCEKCKVLPMLGQLPNQKKLDIDSMDGVRSIGNEFYGIDSNQT 360
           VL N +FVENLV+I+L  C +C+VLPMLGQLPN KKL+I SM+ VRSIG+EFYG+D N  
Sbjct: 747 VLNNNIFVENLVEIRLVDCGRCEVLPMLGQLPNLKKLEIISMNSVRSIGSEFYGVDCNDR 806

Query: 361 HS--FPQLKKLCIDGMKNLEQWGEATL-VPNLFGSLQQVEIYGCEKLTKLPSGIECCSSI 420
           +S  FPQL K  I G+K L+QW EAT+   N FG L+++ + GC +L KLPSG+E C SI
Sbjct: 807 NSSAFPQLNKFHICGLKKLQQWDEATVFASNRFGCLKELILSGCHQLAKLPSGLEGCYSI 866

Query: 421 KYLTIRDCPNLMLNVQNLQNLYHLDMNGLERLP 444
           +YL I  CPNLMLNVQNL NLYHLD+ GL+RLP
Sbjct: 867 EYLAIDGCPNLMLNVQNLYNLYHLDIRGLKRLP 896

BLAST of Spg005059 vs. ExPASy TrEMBL
Match: A0A0A0LBV0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G822360 PE=4 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 3.4e-139
Identity = 287/461 (62.26%), Postives = 346/461 (75.05%), Query Frame = 0

Query: 1   MWIAQGFIQPRE----DLIMEDIGEEYFNFLLSRSLFQDVIKDKNGRIIAFKMHDLIHDI 60
           MWIAQGFIQP +    +L+MEDIGE+YFNFLLSRS+FQDV +D N RI+ FKMHDL+HDI
Sbjct: 454 MWIAQGFIQPGQGRDKNLLMEDIGEQYFNFLLSRSIFQDVTRDANKRIVGFKMHDLMHDI 513

Query: 61  ACAVSNCKKMDSNPYNLSGRQ-EKLRTLIYSNGEFDSKI--VDYTCLRVLVPNTFWTKNL 120
           ACA+S+ + ++SNP NLSG+   KLRTLI  N E  + +   D  CLRVL        +L
Sbjct: 514 ACAISSHQNVESNPNNLSGKSVRKLRTLI-CNDEVINYLNQNDIVCLRVLKVIFQSHTDL 573

Query: 121 PILMDKLLHLRYLDISNIRRYGNELILESISMLYNLQTLKLGHMIISLLKNLGKLVNLRH 180
            I +DKL+HLRYLDIS      N+L+LES+S+LYNLQTLKLG     L KNL KLVNLRH
Sbjct: 574 WIPIDKLIHLRYLDISECSI--NKLLLESLSLLYNLQTLKLGQS--GLPKNLRKLVNLRH 633

Query: 181 LEFE--GPRVIEKMPSHIGKLIHLQTLYQFAVGFEKGCKIEELGPLKNLKGTLMLTNLEN 240
           LEF+  G      MPS +G LIHLQ+L  F VGFEKGCKIEELGPLKNLKG L LTNL  
Sbjct: 634 LEFKMFGDTA---MPSDMGNLIHLQSLSGFLVGFEKGCKIEELGPLKNLKGKLTLTNLWR 693

Query: 241 VKSKEEAISAKLVEKKNLCELNFCWNFCCE--GEYEKCNNLEVLEGLQPHKNLQSLGILY 300
           V++K+EA++AKLVEKKNL  LN  W F  +  GE ++   ++VLEGLQPHKNLQSL IL 
Sbjct: 694 VQNKDEAMAAKLVEKKNLRHLNL-WFFETDKRGEDDEDGIVQVLEGLQPHKNLQSLEILG 753

Query: 301 FGGEVLPNGVFVENLVKIKLRSCEKCKVLPMLGQLPNQKKLDIDSMDGVRSIGNEFYGID 360
           F G+VLP G+FVENLVKI+L   E+C+VLPMLGQLPN K+L+I  M+ VRSIGNEFYG+D
Sbjct: 754 FRGKVLPTGIFVENLVKIRLGHFERCEVLPMLGQLPNLKELEIMYMESVRSIGNEFYGVD 813

Query: 361 SNQTHS--FPQLKKLCIDGMKNLEQWGEATLV--PNLFGSLQQVEIYGCEKLTKLPSGIE 420
           S+  +S  FPQLKKL I  M NLEQW EAT+V   NLFG L++V I  C  L KLPSG+E
Sbjct: 814 SSHQNSVAFPQLKKLSIYEMMNLEQWDEATVVLESNLFGCLKEVRIRRCNPLAKLPSGLE 873

Query: 421 CCSSIKYLTIRDCPNLMLNVQNLQNLYHLDMNGLERLPKGL 447
            C S++YL+IR C NLMLNVQNL  LYHL+++GL+RLPKG+
Sbjct: 874 GCHSLEYLSIRGCFNLMLNVQNLHKLYHLEIDGLKRLPKGM 905

BLAST of Spg005059 vs. ExPASy TrEMBL
Match: A0A6J1CU86 (disease resistance protein RGA2-like OS=Momordica charantia OX=3673 GN=LOC111014619 PE=4 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 2.9e-130
Identity = 267/461 (57.92%), Postives = 331/461 (71.80%), Query Frame = 0

Query: 1   MWIAQGFIQPRE-----DLIMEDIGEEYFNFLLSRSLFQDVIKDKNGRIIAFKMHDLIHD 60
           +WIAQGFIQP++     ++  EDIGE +FNFLLSRSLFQDV  DK+ R I F MHDLIHD
Sbjct: 445 IWIAQGFIQPQDQEGDNEMTEEDIGEWHFNFLLSRSLFQDVTMDKSERGIYFIMHDLIHD 504

Query: 61  IACAVSNCKKM-----DSNPYNLSGRQEKLRTLIYSNGEFDSKIVDYTCLRVLVPNTFWT 120
           IACA+SN +K+      SN    S R  KLRTLI +N    +++VD   LRVLV +   T
Sbjct: 505 IACAISNHQKLLHELSSSNRSRKSAR--KLRTLICNNQVIYNELVDCVSLRVLVLHCEKT 564

Query: 121 KNLPILMDKLLHLRYLDISNIRRYGN--ELILESISMLYNLQTLKLGHMIISLLKNLGKL 180
            NL   + KL HLRYL I+N    GN  +++ ESI MLYNLQTLKL HM  SL KN  K+
Sbjct: 565 GNLLESIGKLKHLRYLSIAN----GNYVDVLPESIVMLYNLQTLKLRHM-KSLPKNSRKM 624

Query: 181 VNLRHLEFEGPRVIEKMPSHIGKLIHLQTLYQFAVGFEKGCKIEELGPLKNLKGTLMLTN 240
           V+LRHLEFE      +MPSH+ +LIHLQTL +F  GFE GCKIEELGPLKNLKG L L+ 
Sbjct: 625 VSLRHLEFELKSDFRQMPSHMSELIHLQTLSRFVAGFENGCKIEELGPLKNLKGKLQLSC 684

Query: 241 LENVKSKEEAISAKLVEKKNLCELNFCWNFCCEGEYEKCNNLEVLEGLQPHKNLQSLGIL 300
           LE V+SKEE+I AKLVEKKNL EL F W+   EG+  + + LEVLEGLQPHKNLQSL ++
Sbjct: 685 LERVESKEESIVAKLVEKKNLQELIFEWSSRFEGKNSRYSELEVLEGLQPHKNLQSLTVI 744

Query: 301 YFGGEVLPNGVFVENLVKIKLRSCEKCKVLPMLGQLPNQKKLDIDSMDGVRSIGNEFYGI 360
            FGG+VL + +FVENL  I+L +C+ C+ LPMLG+LPN  +L+I+SMD +R IG+EFYG 
Sbjct: 745 GFGGQVLGDAIFVENLTVIRLSNCKNCERLPMLGRLPNLVELNINSMDSLRCIGSEFYGN 804

Query: 361 DS-NQTHSFPQLKKLCIDGMKNLEQWGEATLVPNLFGSLQQVEIYGCEKLTKLPSGIECC 420
            S NQ HSF +L+KL     +NLEQW +A +V N FGSLQ + IYGC  L KLP+G+E C
Sbjct: 805 GSNNQGHSFSKLRKLTFIWCRNLEQWKDAAVVSNSFGSLQTLSIYGCGNLEKLPNGLEGC 864

Query: 421 SSIKYLTIRDCPNLMLNVQNLQNLYHLDMNGLERLPKGLAH 449
           SS++ LT+  CP L LNVQN QNL +L ++GL++LP GL H
Sbjct: 865 SSLRDLTVAYCPKLSLNVQNCQNLSNLRIDGLKKLPLGLGH 898

BLAST of Spg005059 vs. ExPASy TrEMBL
Match: A0A6J1CVR6 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111014685 PE=4 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 8.4e-130
Identity = 265/455 (58.24%), Postives = 320/455 (70.33%), Query Frame = 0

Query: 1   MWIAQGFIQPRE---DLIMEDIGEEYFNFLLSRSLFQDVIKDKNGRIIAFKMHDLIHDIA 60
           MWIAQGFIQ  E   +   EDIGE + NFLLSRSLFQDV+KD N R+  FKMHDLIHDIA
Sbjct: 445 MWIAQGFIQLHEGHNETTEEDIGEWHLNFLLSRSLFQDVVKDMNERVTHFKMHDLIHDIA 504

Query: 61  CAVSNCKKM----DSNPYNLSGRQEKLRTLIYSNGEFDSKIVDYTCLRVLVPNTFWTKNL 120
           CA+SN  K+     SN    S R  KLRTLI +N    ++++D   LRVLV N+  T NL
Sbjct: 505 CAISNHPKLLELGSSNWSRKSAR--KLRTLICNNQVIYNELMDCVSLRVLVLNSAKTDNL 564

Query: 121 PILMDKLLHLRYLDISNIRRYGNELILESISMLYNLQTLKLGHMIISLLKNLGKLVNLRH 180
              + KL HLRYLDIS+   Y   ++ ESI+ LYNLQTLK+G M  SL KNL K+V+LRH
Sbjct: 565 LDSIGKLKHLRYLDISH---YSIRVLPESIAKLYNLQTLKIGRM-ESLPKNLRKMVSLRH 624

Query: 181 LEFEGPRVIEKMPSHIGKLIHLQTLYQFAVGFEKGCKIEELGPLKNLKGTLMLTNLENVK 240
           L+F       +MPSH+  LIHLQTLY+FA GFE GCKIEELGPLKNLKG L L+NLE VK
Sbjct: 625 LKFYHGYHTMQMPSHMSHLIHLQTLYEFAAGFENGCKIEELGPLKNLKGRLRLSNLERVK 684

Query: 241 SKEEAISAKLVEKKNLCELNFCWNFCCEGEYEKCNNLEVLEGLQPHKNLQSLGILYFGGE 300
           SKEEAI+AKLVEKKNL EL F W+   E      ++ EVLEGLQPHKNLQSL I+ FGG+
Sbjct: 685 SKEEAIAAKLVEKKNLHELTFDWSLDFE---RNDDDSEVLEGLQPHKNLQSLNIMNFGGQ 744

Query: 301 VLPNGVFVENLVKIKLRSCEKCKVLPMLGQLPNQKKLDIDSMDGVRSIGNEFYGIDSNQT 360
           VLP  +FVENL  I+L   +KC++LPMLGQLPN  +L+I  MD VR IG+EFYG      
Sbjct: 745 VLPEAIFVENLAVIRLCGGKKCEMLPMLGQLPNLVELEISFMDSVRCIGSEFYG-----N 804

Query: 361 HSFPQLKKLCIDGMKNLEQWGEATLVPNLFGSLQQVEIYGCEKLTKLPSGIECCSSIKYL 420
            SF +L+K    G+ NLEQW EA  + N FGSLQ + + GC  L KLP+G+E CSS+  L
Sbjct: 805 GSFSKLRKFDFIGLHNLEQWTEAASISNSFGSLQTLNVIGCGNLKKLPNGLEFCSSLHDL 864

Query: 421 TIRDCPNLMLNVQNLQNLYHLDMNGLERLPKGLAH 449
           T+RDCP L LNVQN+ NL +L ++G + LP GL+H
Sbjct: 865 TVRDCPKLALNVQNMHNLSNLFIHGFKELPLGLSH 885

BLAST of Spg005059 vs. ExPASy TrEMBL
Match: A0A5A7TNC1 (Disease resistance protein RGA2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003650 PE=4 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 4.3e-126
Identity = 262/455 (57.58%), Postives = 317/455 (69.67%), Query Frame = 0

Query: 1   MWIAQGFIQP--REDLIMEDIGEEYFNFLLSRSLFQDVIKDKNGRIIAFKMHDLIHDIAC 60
           MWIAQGFIQP   E+++MED+GE YFNFLLSRSLFQDV+KDK GRI  FKMHDLIHD+A 
Sbjct: 446 MWIAQGFIQPPEGENMMMEDLGEGYFNFLLSRSLFQDVVKDKYGRITHFKMHDLIHDVAL 505

Query: 61  AV-SNCKKMDSNPYNLSGR-QEKLRTLIYSNGEFDSKIVDYTCLRVLVPNTF-WTKNLPI 120
           A+ SN +KM  +P + +G+   KLRTLIY+N E   K+ D   LRVL  N+     NLP 
Sbjct: 506 AILSNRQKMVLDPTHWNGKTARKLRTLIYNNQEIHHKLADCVFLRVLEVNSLRLMNNLPD 565

Query: 121 LMDKLLHLRYLDISNIRRYGNELILESISMLYNLQTLKLGHMIISLLKNLGKLVNLRHLE 180
            + KL +LRYLDIS+   +    I  SI+ L+NLQTLKLG  I +L  NL  LV LRHLE
Sbjct: 566 FIAKLKYLRYLDISSCPMWA---IPHSITTLFNLQTLKLG-SIENLPMNLRNLVRLRHLE 625

Query: 181 FEGPRVIEKMPSHIGKLIHLQTLYQFAVGFEKGCKIEELGPLKNLKGTLMLTNLENVKSK 240
           F       KMP H+G+LIHLQTL  F  GFE+GCKIEELG LKNL+G L L+NLE V+SK
Sbjct: 626 FHVYYNTRKMPPHMGELIHLQTLSGFVAGFEEGCKIEELGNLKNLRGKLQLSNLEQVRSK 685

Query: 241 EEAISAKLVEKKNLCELNFCWNFCCEGEYEKCNNLEVLEGLQPHKNLQSLGILYFGGEVL 300
           +EAI+AKLV KKNL EL F W+     E+   N+ EVLEGLQP KNL SL I  FGG+ L
Sbjct: 686 QEAIAAKLVNKKNLRELTFEWSIDLLREFSSYNDFEVLEGLQPPKNLTSLKITNFGGKFL 745

Query: 301 PNGVFVENLVKIKLRSCEKCKVLPMLGQLPNQKKLDIDSMDGVRSIGNEFYGIDSNQTHS 360
           P G FVENL  + L  C KC+ LPMLGQLPN ++L I  MD V SIGNEFYG DSN+   
Sbjct: 746 PAGTFVENLAFLCLYGCTKCERLPMLGQLPNLQELGICFMDRVISIGNEFYGNDSNRRGY 805

Query: 361 FPQLKKLCIDGMKNLEQW--GEATLVPNLFGSLQQVEIYGCEKLTKLPSGIECCSSIKYL 420
           FP+LKK  +  M NLEQW    A    N FGSLQ +++  C KLTKLP+G+ECC S++ +
Sbjct: 806 FPKLKKFDLGWMCNLEQWELEVANHESNHFGSLQTLKLDRCGKLTKLPNGLECCKSVREV 865

Query: 421 TIRDCPNLMLNVQNLQNLYHLDMNGLERLPKGLAH 449
            I  CPNL LNV+ + +L  L ++GL+ LPKGLAH
Sbjct: 866 IISSCPNLTLNVEKMHSLSVLLIDGLKFLPKGLAH 896

BLAST of Spg005059 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 169.5 bits (428), Expect = 6.5e-42
Identity = 142/453 (31.35%), Postives = 214/453 (47.24%), Query Frame = 0

Query: 1   MWIAQGFI-QPREDLIMEDIGEEYFNFLLSRSLFQDVIKDKNGRIIAFKMHDLIHDIACA 60
           +W+A+GF+ Q R    +E++G EYF+ L SRSL Q   K K   I    MHD I+++A  
Sbjct: 450 LWMAEGFLQQTRSSKNLEELGNEYFSELESRSLLQ---KTKTRYI----MHDFINELAQF 509

Query: 61  VS---------NCKKMDS--------------NPYNLSGRQEK--LRTLIYSNGEFDSK- 120
            S          CK   S               P      +E   LRT +  +    S+ 
Sbjct: 510 ASGEFSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFLRTFLPLSLTNSSRS 569

Query: 121 -----------IVDYTCLRVLVPNTFWTKNL-PILMDKLLHLRYLDISNIRRYGNELILE 180
                      +   T LRVL  + +    L P     + H R+LD+S   R   E + +
Sbjct: 570 CCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLS---RTELEKLPK 629

Query: 181 SISMLYNLQTLKLGH--MIISLLKNLGKLVNLRHLEFEGPRVIEKMPSHIGKLIHLQTLY 240
           S+  +YNLQTL L +   +  L  ++  L+NLR+L+  G + + +MP   G+L  LQTL 
Sbjct: 630 SLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIGTK-LRQMPRRFGRLKSLQTLT 689

Query: 241 QFAVGFEKGCKIEELGPLKNLKGTLMLTNLENVKSKEEAISAKLVEKKNLCELNFCWNFC 300
            F V    G +I ELG L +L G L +  L+ V    +A  A L  KK+L E++F W   
Sbjct: 690 TFFVSASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLNSKKHLREIDFVWRTG 749

Query: 301 CEGE------YEKCNNLEVLEGLQPHKNLQSLGILYFGGEVLPNGV---FVENLVKIKLR 360
                     +   N  EV E L+PH++++ L I  + G   P+ +       +V I+LR
Sbjct: 750 SSSSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLR 809

Query: 361 SCEKCKVLPMLGQLPNQKKLDIDSMDGVRSIGNEFYGID----SNQTHSFPQLKKLCIDG 399
            C+ C  LP LGQLP  K+L I  M G++SIG +FY  D          F  L+ L  D 
Sbjct: 810 ECQYCTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDN 869

BLAST of Spg005059 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 164.5 bits (415), Expect = 2.1e-40
Identity = 146/505 (28.91%), Postives = 240/505 (47.52%), Query Frame = 0

Query: 1   MWIAQGFI-QPREDLIMEDIGEEYFNFLLSRSLFQDVIKDKNGRIIAFKMHDLIHDIACA 60
           +W+A   + QPR    +EDIG +Y   L+++S FQ +    +  + +F MHDL++D+A A
Sbjct: 442 LWMAIDLLYQPRSSRRLEDIGNDYLGDLVAQSFFQRL----DITMTSFVMHDLMNDLAKA 501

Query: 61  VSN--CKKMD----------SNPYNLSGRQ-------------EKLRTLIYSNG------ 120
           VS   C +++          +  ++ S  Q             E LRT++  N       
Sbjct: 502 VSGDFCFRLEDDNIPEIPSTTRHFSFSRSQCDASVAFRSICGAEFLRTILPFNSPTSLES 561

Query: 121 -EFDSKIVD-----YTCLRVLVPNTFWTKNLPILMDKLLHLRYLDISNIRRYGNELILES 180
            +   K+++      + LR+L  + +   NLP  +  L  LRYLD+S+ +    + + E 
Sbjct: 562 LQLTEKVLNPLLNALSGLRILSLSHYQITNLPKSLKGLKLLRYLDLSSTK---IKELPEF 621

Query: 181 ISMLYNLQTLKLGHM--IISLLKNLGKLVNLRHLEFEGPRVIEKMPSHIGKLIHLQTLYQ 240
           +  L NLQTL L +   + SL K++ +L+NLR L+  G  ++E MP  I KL  LQ L  
Sbjct: 622 VCTLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLVGTPLVE-MPPGIKKLRSLQKLSN 681

Query: 241 FAVGFEKGCKIEELGPLKNLKGTLMLTNLENVKSKEEAISAKLVEKKNLCELNFCWNFCC 300
           F +G   G  + EL  L +L+GTL ++ L+NV    EA  A L  K  L  L   W    
Sbjct: 682 FVIGRLSGAGLHELKELSHLRGTLRISELQNVAFASEAKDAGLKRKPFLDGLILKWTVKG 741

Query: 301 EG------EYEKCNNLEVLEGLQPHKNLQSLGILYFGGEVLPNGVFVEN---LVKIKLRS 360
            G          C+  EVL  L+PH +L++  I  + G   P  +   +   +  + L S
Sbjct: 742 SGFVPGSFNALACDQKEVLRMLEPHPHLKTFCIESYQGGAFPKWLGDSSFFGITSVTLSS 801

Query: 361 CEKCKVLPMLGQLPNQKKLDIDSMDGVRSIGNE-FYGIDSNQTHSFPQLKKLCIDGMKNL 420
           C  C  LP +GQLP+ K L I+  + ++ +G + F+G ++++   F  L+ L   GM   
Sbjct: 802 CNLCISLPPVGQLPSLKYLSIEKFNILQKVGLDFFFGENNSRGVPFQSLQILKFYGMPRW 861

Query: 421 EQWGEATLVPNLFGSLQQVEIYGCEKL-TKLPSGIECCSSIKYLTIRDCPNLMLNVQNLQ 455
           ++W    L   +F  LQ++ I  C  L  K P G+   + +   TI DCP  +  V   +
Sbjct: 862 DEWICPELEDGIFPCLQKLIIQRCPSLRKKFPEGLPSSTEV---TISDCP--LRAVSGGE 921

BLAST of Spg005059 vs. TAIR 10
Match: AT1G69550.1 (disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 75.5 bits (184), Expect = 1.3e-13
Identity = 99/380 (26.05%), Postives = 161/380 (42.37%), Query Frame = 0

Query: 113  LPILMDKLLHLRYLDISNIRRYGNELILESISMLYNLQTLKLG--HMIISLLKNLGKLVN 172
            LP  +  L+ L+ L +  I       I  SI  L NL+ L L     ++ L  ++G L+N
Sbjct: 828  LPSSIGNLISLKILYLKRISSLVE--IPSSIGNLINLKLLNLSGCSSLVELPSSIGNLIN 887

Query: 173  LRHLEFEGPRVIEKMPSHIGKLIHLQTLYQFAVGFEKGCKIEELGPLKNLKGTLMLTNLE 232
            L+ L+  G   + ++P  IG LI+LQ LY           + E   L  L  +  + NL 
Sbjct: 888  LKKLDLSGCSSLVELPLSIGNLINLQELY-----------LSECSSLVELPSS--IGNLI 947

Query: 233  NVKSKEEAISAKLVEKK----NLCELNFCWNFCCEGEYEKCNNLEVLEGLQPHK------ 292
            N+K+   +  + LVE      NL  L   +   C    E  +++  L  L+         
Sbjct: 948  NLKTLNLSECSSLVELPSSIGNLINLQELYLSECSSLVELPSSIGNLINLKKLDLSGCSS 1007

Query: 293  ------------NLQSLGILYFGGEV-LPNGV-FVENLVKIKLRSCEKCKVLP-MLGQLP 352
                        NL++L +      V LP+ +  + NL ++ L  C     LP  +G L 
Sbjct: 1008 LVELPLSIGNLINLKTLNLSECSSLVELPSSIGNLINLQELYLSECSSLVELPSSIGNLI 1067

Query: 353  NQKKLDIDSMDGV----RSIGNEFYGIDSNQTHSFPQLKKLCIDGMKNLEQWGEATLVPN 412
            N KKLD+     +     SIGN               LK L + G  +L +      +P+
Sbjct: 1068 NLKKLDLSGCSSLVELPLSIGN------------LINLKTLNLSGCSSLVE------LPS 1127

Query: 413  LFG--SLQQVEIYGCEKLTKLPSGIECCSSIKYLTIRDCPNLML------NVQNLQNLYH 454
              G  +L+++++ GC  L +LPS I    ++K L +  C +L+       N+ NLQ LY 
Sbjct: 1128 SIGNLNLKKLDLSGCSSLVELPSSIGNLINLKKLDLSGCSSLVELPLSIGNLINLQELYL 1174

BLAST of Spg005059 vs. TAIR 10
Match: AT3G50950.2 (HOPZ-ACTIVATED RESISTANCE 1 )

HSP 1 Score: 73.6 bits (179), Expect = 4.9e-13
Identity = 91/346 (26.30%), Postives = 146/346 (42.20%), Query Frame = 0

Query: 2   WIAQGFIQPREDLIMEDIGEEYFNFLLSRSLFQDVIKDKNGRIIAFKMHDLIHDIACAVS 61
           WI +GF+  R      + GE+ F+ L +R L + V K  +G II  K+HD++ D+   ++
Sbjct: 440 WIGEGFVMWRNGRSATESGEDCFSGLTNRCLIEVVDKTYSGTIITCKIHDMVRDLVIDIA 499

Query: 62  NCKKMDSNPYNLSGR---------------QEKLRTLIYS---------NGEFDSKIVDY 121
             K   SNP  L+ R                 KLR ++ +         N +   K  D 
Sbjct: 500 K-KDSFSNPEGLNCRHLGISGNFDEKQIKVNHKLRGVVSTTKTGEVNKLNSDLAKKFTDC 559

Query: 122 TCLRVL-VPNTFWTKNLPILMDK---LLHLRYLDISNIRRYGNELIL--ESISMLYNLQT 181
             LRVL +  + +   L  ++D+   L HL  L +SN     + LI    S+  L+NLQ 
Sbjct: 560 KYLRVLDISKSIFDAPLSEILDEIASLQHLACLSLSNT----HPLIQFPRSMEDLHNLQI 619

Query: 182 LKLGHMIISLLKNLGKLV----NLRHLEFEGPRVIEKMPSHIGKLIHLQTLYQF-AVGFE 241
           L   +     LK L   +     L  L+      +E  P  IG L+ L+ L  F      
Sbjct: 620 LDASY--CQNLKQLQPCIVLFKKLLVLDMTNCGSLECFPKGIGSLVKLEVLLGFKPARSN 679

Query: 242 KGCKIEELGPLKNL-KGTLMLTNLENVKSKEEAISAKLVEKKNLCELNFCWNFCCEGEYE 301
            GCK+ E+  L NL K  L LT  + ++ +EE  S   + K     +N      C   Y 
Sbjct: 680 NGCKLSEVKNLTNLRKLGLSLTRGDQIE-EEELDSLINLSKLMSISIN------CYDSYG 739

Query: 302 KCNNLEVLEGLQPHKNLQSLGILYFGGEVLPNGVFVENLVKIKLRS 312
             + +  ++ L P   L  L + ++ G+  P+ +    L  ++  S
Sbjct: 740 D-DLITKIDALTPPHQLHELSLQFYPGKSSPSWLSPHKLPMLRYMS 770

BLAST of Spg005059 vs. TAIR 10
Match: AT3G50950.1 (HOPZ-ACTIVATED RESISTANCE 1 )

HSP 1 Score: 73.6 bits (179), Expect = 4.9e-13
Identity = 91/346 (26.30%), Postives = 146/346 (42.20%), Query Frame = 0

Query: 2   WIAQGFIQPREDLIMEDIGEEYFNFLLSRSLFQDVIKDKNGRIIAFKMHDLIHDIACAVS 61
           WI +GF+  R      + GE+ F+ L +R L + V K  +G II  K+HD++ D+   ++
Sbjct: 440 WIGEGFVMWRNGRSATESGEDCFSGLTNRCLIEVVDKTYSGTIITCKIHDMVRDLVIDIA 499

Query: 62  NCKKMDSNPYNLSGR---------------QEKLRTLIYS---------NGEFDSKIVDY 121
             K   SNP  L+ R                 KLR ++ +         N +   K  D 
Sbjct: 500 K-KDSFSNPEGLNCRHLGISGNFDEKQIKVNHKLRGVVSTTKTGEVNKLNSDLAKKFTDC 559

Query: 122 TCLRVL-VPNTFWTKNLPILMDK---LLHLRYLDISNIRRYGNELIL--ESISMLYNLQT 181
             LRVL +  + +   L  ++D+   L HL  L +SN     + LI    S+  L+NLQ 
Sbjct: 560 KYLRVLDISKSIFDAPLSEILDEIASLQHLACLSLSNT----HPLIQFPRSMEDLHNLQI 619

Query: 182 LKLGHMIISLLKNLGKLV----NLRHLEFEGPRVIEKMPSHIGKLIHLQTLYQF-AVGFE 241
           L   +     LK L   +     L  L+      +E  P  IG L+ L+ L  F      
Sbjct: 620 LDASY--CQNLKQLQPCIVLFKKLLVLDMTNCGSLECFPKGIGSLVKLEVLLGFKPARSN 679

Query: 242 KGCKIEELGPLKNL-KGTLMLTNLENVKSKEEAISAKLVEKKNLCELNFCWNFCCEGEYE 301
            GCK+ E+  L NL K  L LT  + ++ +EE  S   + K     +N      C   Y 
Sbjct: 680 NGCKLSEVKNLTNLRKLGLSLTRGDQIE-EEELDSLINLSKLMSISIN------CYDSYG 739

Query: 302 KCNNLEVLEGLQPHKNLQSLGILYFGGEVLPNGVFVENLVKIKLRS 312
             + +  ++ L P   L  L + ++ G+  P+ +    L  ++  S
Sbjct: 740 D-DLITKIDALTPPHQLHELSLQFYPGKSSPSWLSPHKLPMLRYMS 770