Spg004673 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg004673
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	tRNA (guanine(26)-N(2))-dimethyltransferase
Location	scaffold5: 20230962 .. 20236310 (+)
RNA-Seq Expression	Spg004673
Synteny	Spg004673

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GCCTTTAGCCGTGGATTGGAGATTAGGGTTTAAGGCAAAAGATTGAAAAATATTTATCGTGCACAGAATTTGAGTTAACTTTCAATTTTCGAGTCGCAGTTGAGTGGCAATAGGGTTCCGTTGTACAAAATTACAGTTCTTAAATCATATTGGACATATTTAAGGGTTGGTTACTGCTTGATGATTGATTGGTTTTGTTTATGTAACTGCTGCTCTAGTTCAAGTTCTGAAACTCTCTCACAAAGGTCTTAGGAGCAAAAGAAGCGGAGACATTGGAGCTTTTAAAGGGTTGTGGATTTTGCTTTGACCCTTGAAAGAAGAATCCCAGAATCTGTTTTTTTGCATGTGCGACTGAGAGCCGCAATAATGGCGACAGATCTTAGTGAATTCACCATCATCAAGGAGGGAGAGGCTGAGATTCTCATGCACGCGAAGAATGAAGTCTTTTTCAATAAGACCCAGGTGCCTTGTTTATTTAAATAATGGTTTTTTTGGAGTTTTGGAGTTTAGGCATCGTTCTTAATTAAGATTAATCGGTCAAGTCAAAAGTTTCATGGATTCTAGGTATGGGGAAAGAATTTACATGTGATTGTTTCCAGCATTTGAAGTTTGGCGTGGTTATTGATTGGGGAAATATTTAAGATTTGACTTCTATTTTTGTTTAAGTGGGAAAATTTCATGAGGAGTTTAGGGAAAACTTCTATTTTAGTGAAGTGAAGAGGTTTTGTAATTGTTGCTACTTACTTGAAACTTCAGCATATTAGCATATTAAGTGCTTGATATTTGATGGAGAAACTAATATATACAATTCATTCAAGTCAGCAAAGTATAATGTGGTATGATAAGTTCAAAGATATTGTTCCATATAACAATTACACCATGACGACAATCTCTTTAATCTTATGAATAACTTATCGTTCAAAGATTCTTAGTAGCAGCATTAGCTAAATTGTCTGCACTGCTTTAACTACATAATTTGTTTTTAGTCAAAGCAGAAGTCTTCCCCTCAGCAATTAAGTATATACGGTTTTTTTTTTCTTCCTTTCATGAAAACTATTACATACTCTTTTTTTATCTGTAATATCGTCATTGAATAGTGATTGTTGGATTTTCTTTTCTATACTGCCCATGTATACCTAGCTTCAAGCCTTTTCTCATTGTCCAATCTTGATAGTTCCACCTTTGTAATACTCAGGTTAACAACAGAGACATATCCATTGCTGTCCTTAGGGCATTTATATCAAAACGCAAGCAGGAGAATGAAACAAAACTATCAAGAAGAGCAAAATCAGGGCAAAAGATTTCTGAAACTAAAGGTTCTGAAACTGTTATGGAAGCAGCATCTAACGAATCTGCAACAAATGATGAAAAGTGCAATACAGATTGTGATGCTCATGAAAAAACATCAAATGAATGTGGAAGTCCGATGTTGTCAGAAGAACACAAAGAGATCACAGAGGGAAAAGAACAAAGAGATCCCAAGCCTTTTAGAGTTCTTGAGGTTCTCTTAATTTTATTTAATTTCATTTATCATTCTATTATGTGCATGTGCATAGTTTACTCGAAGTTTATTGTCCAATGAAATGTAGAGTGTAGACATTTCAATTTAGAATTATGTTCATGCCTGGCTTATTAGCCCTCTGCTTGAGTAATATAAAGTTTATAATCTGTCTGTTCCCCTACAAAGGCAAACAAAATTTTGAAATGATATTACTATTAGGAAGGACCTTCTAGAGATGCTCAACAAGAAGTTTGACGAGAATTTATTAATTAGCAGTGGATAGACTCAGAGGCCAGAAGTTTCTCCTCTCTTACATTGGAATTTTATGAACAAGATGACTGAGAGTTTCCTGAGTACTAAAATTGAAGATTCATTTATGACAAAAGTTTTAGAACAATGCAACATAACTGGGGGCTTTTCCAACTCCAAGTTCTTAGATGGTTGCAAGATTTATTATCCACCAAATATCTTCTCCACTTGTTAGATACCTTGAGTTGAATTGGCCACTAGGACGTAAGTTTTTTTGAACAAATGGAAGACCAAGGGAAAGGGTAAGGACATGGCAGAAAAAAAAAATTATATAAAACAATGAATTATGAATGAAGATAGAACTACGTATCTGATATTTTTGCTTCTTTTATGATAGTTGAGTAAGTTTAGAGTTTCTCTTATTTTTATTTTAAATCTGTTCTTTTTTGGAATTGAAGTATTATATATTTTTATTTTTGTTACAAATCTGATTTTAGATTTCCTATACATATTTATTCTATTCATCACTGCAAACATTATGTAATTTACTTCTCAAATTCTTACAGTGCTGTATAGCTTATCATCTCATTCATATGGTAGTTGGAATTTCGAGTATCACAATTTAAATTGCCATCTCTTATGTGAAAATTATGTAGATGCGTCAAAAGATACAAAAACATTAGATAAAAGTTGTTCTACATAGAATATGAGCGTGTCCCTCTTCTAACATATTAGAATTTGTTGTTCAACATGTTCACACTGTGTTTCTTGACTCTGATTCCGATGTTATATTTGTGCTTCTAAAATTTTGGTTGTGTCTGTTTGCTTATCTTTTGGGATGCATATATGTTGTCCATTTAATGCTTTCTGAGTTTTTCCTGTTTAAATTTGAAGGCCTTGTCAGCTTCTGGGCTAAGAGCCTTAAGATATGCTCGGGAAGTAGAGGGGATTGACAAAGTTGTGGCGCTAGACAATGATAAAGGTAATTTTTTAATTATTGGTAATATACTAATATCAAAAAGTTCTTTTTAGCAAATATTACTTGGCCATTCTTTGGGAGGAGGAGGGGTAAGAATTTGGTTAGTAACCAAAAATACTTACTTAAATAACGTGGAAACAATTATTGACTTACTGTGTTCCAGCCTGAAACATAGTTTCATGTCTGATATGAGTATATTGTTCCAAGTATATTGTTTTTTTAATATATATAGATATTTTGGTTGGAGGGGGTGGGGGGAAGAACATTTTATCTTATTGCCCTTTATTTTATTTCTTTTTTCTTTTTTTGGAATAAAATTTTAATTGTCTAAAAGAAAAGAAGGGATTAAAAATCTTGCAAGGAGTCAAAAGTATATGAGGTTGTTTGGTTGTCCGTTTCTCATATCAAGTGATACCTTTGTATGCACACTTCATTTGTGCCGTGCCAAAGAGCGCAAAGTGGTTACTGATCATGAGGCGTTCTGCTTTTACAAGGTATTAAGTGGAATTAGGAATGTCACTTTTGGTTGACACTATCCCTAATATTTAAGGCAACCTTGTCTTTTGCAAGCAGAGGAAGTTGGTGTTGATAATTCATTCCCCTTTTTATCTGTGTTGATTTATGTTTCATCTTCTGGAGAGCATGTACTAATGTCTCAGATTGCCAGTGGAACCTTAGAAATGGTAGTTATCCATTTTTCTAATCTTACAAATATGCTATTATCTATATTGATATGCCATTTAGGTGTTTCTTTTTAGTTATCGGTTACTAATTAACCTGTTAACGATAAGAATTGTTTTTCAATAGCATCGGTTGAAGCTTGTCGAAGAAACATCAAGTTTAATGGTTCAGTAGCATGTGCGAAAGTGGAATCACATCTTACTGATGCTCGTGTTTATATGCTCACTCACCCAAAAGAATTTGATATGGTATGCATGCACTATTTCAGATATGTAGGTTAGCCCTTTAAAAATTAAACATAGGGAAGTAACTATTGAAATGCGATTTCTTTTTCCTTATTACAGTTTAGAATAGTAACAAATTGAATAATTATGTATAACGTAATGATATGAGCACAGACGTGAATACAAGATTGAAGCTTTTATGCCACTTATTTGGATTGTTTCAGGTTGATCTGGATCCTTACGGTTCACCTTCTGTTTTCTTGGACTCTGCCGTTCAATCAGTTGCTGATGGTGGCATGCTGATGTGCACAGCAACTGATATGGCAGTGCTTTGTGGGGGAAATGGAGAAGTTTGCTATTCAAAGTAAGGTTTTTTACAATCCTCAATTATCATTCTTATTTTGAAGTTCTATTGTAATAGATTTTGTGTCCTAAAAATTGTTGATTGATTCAGATATGGTTCCTATCCTTTGAGGGGGAAATATTGCCACGAAATGGCTTTAAGAATCGTTCTTGCCTGCATTGAGGTATACCTATCCCTTGTGTATTTGAAAAGTTTTCTTAATGAATTATTTTACCACCTTTTATTTTTTCTTCTTTGGTTTAACATGCTGTACAGCGTACAGGATCATTCTTATCTTAATTAAAAGATACAGTATAGGATCATAAAGGACACACACACTATAGGAAACTGAAAATCTATTACAAAGGAGAGGTGTACTAAAGGATGGGAGGAGAAATCTCTCAAGGCAAAGGGATTTACAAAAAAGGATCCCAATTTGCACAAATAAGCAAAATATTGTAATTAAAAAAGAATAGGACAGAGGTACACACCAAGAAGCATAGTCTAAGTAGGTCGGTCCAACATTCTTTCAAAGGAGTAAAAGAGTCTTTGAAAATCTTTTTTCCTATCTAAGCCTCCGTAAATGAGGGTATTAGTCCAAAGGATTGTGGATTTCCTGGGTGAAAAGGTGGCAATGCAGAATCTGAGCTATCATCGATCTGCAGCATGAAGAGAAACACCAAGCAATTCTTGTTTACGAGCTTTTATCAATCGAGAAATATAAGATGGATATCCAAGTTGATACCCTCAGTATTATCAATTAAAATATTTGGCTCGGCCTGTTTATGATTATGAGCATTTTGCACTGAATTAGGGAATCTGAATCTAAATCTGAGAATTAGGGAATGTGAATCTAAATCTGAGAAATAGAGCGCTGCTAGCTAAATGGGTATGGTAGTTCCCGCTTGAGTCGACCTCCTGATGGCACTGGATTATTGTTAGTAAGTATGACTATCATCCCTATGAGTGGCTAATGGGTGGGGTTAAAGGCATGGGAAGAAATCTGTGGAAAGATATTGTTTACGAGCTCCTGGCTTTTTTGAGTGTAGTTCATTGTGTTGTGGATGATGGGAGAGATACTTATTTTTGGGAGGATATTTGGGTGGGGGATAGTTCACCCCGCTCCAAGTTTCCTAATCTCTATCAATTGTCCTCCATGAAAAATCGGGTGATTTCTGAGGTTATTACTGTCTTCGGGGGCTCTCCCTCTTATTCTTTGGGTTTCCATCTCTCTTTGACCGATAAGGAAGCGATGAAGGTCGTAGCTCTCGTGTCTTTGATCGGGGAGTTTCAGTTTAGGGATGGGAGGAGGGATATTCGTAGTTGGAGTCTCAGCCCTTCTAAGGGCTTCTCGTGTAGTTCCTTTTTTTAG

mRNA sequence

ATGGCGACAGATCTTAGTGAATTCACCATCATCAAGGAGGGAGAGGCTGAGATTCTCATGCACGCGAAGAATGAAGTCTTTTTCAATAAGACCCAGGTTAACAACAGAGACATATCCATTGCTGTCCTTAGGGCATTTATATCAAAACGCAAGCAGGAGAATGAAACAAAACTATCAAGAAGAGCAAAATCAGGGCAAAAGATTTCTGAAACTAAAGGTTCTGAAACTGTTATGGAAGCAGCATCTAACGAATCTGCAACAAATGATGAAAAGTGCAATACAGATTGTGATGCTCATGAAAAAACATCAAATGAATGTGGAAGTCCGATGTTGTCAGAAGAACACAAAGAGATCACAGAGGGAAAAGAACAAAGAGATCCCAAGCCTTTTAGAGTTCTTGAGGCCTTGTCAGCTTCTGGGCTAAGAGCCTTAAGATATGCTCGGGAAGTAGAGGGGATTGACAAAGTTGTGGCGCTAGACAATGATAAAGCATCGGTTGAAGCTTGTCGAAGAAACATCAAGTTTAATGGTTCAGTAGCATGTGCGAAAGTGGAATCACATCTTACTGATGCTCGTGTTTATATGCTCACTCACCCAAAAGAATTTGATATGGTTGATCTGGATCCTTACGGTTCACCTTCTGTTTTCTTGGACTCTGCCGTTCAATCAGTTGCTGATGGTGGCATGCTGATGTGCACAGCAACTGATATGGCAGTGCTTTGTGGGGGAAATGGAGAAGTTTGCTATTCAAAATATGGTTCCTATCCTTTGAGGGGGAAATATTGCCACGAAATGGCTTTAAGAATCGTTCTTGCCTGCATTGAGTATGACTATCATCCCTATGAGTGGCTAATGGGTGGGGTTAAAGGCATGGGAAGAAATCTGTGGAAAGATATTGTTTACGAGCTCCTGGCTTTTTTGAGTGTAGTTCATTGTGTTGTGGATGATGGGAGAGATACTTATTTTTGGGAGGATATTTGGGTGGGGGATAGTTCACCCCGCTCCAAGTTTCCTAATCTCTATCAATTGTCCTCCATGAAAAATCGGGTGATTTCTGAGGTTATTACTGTCTTCGGGGGCTCTCCCTCTTATTCTTTGGGTTTCCATCTCTCTTTGACCGATAAGGAAGCGATGAAGGTCGTAGCTCTCGTGTCTTTGATCGGGGAGTTTCAGTTTAGGGATGGGAGGAGGGATATTCGTAGTTGGAGTCTCAGCCCTTCTAAGGGCTTCTCGTGTAGTTCCTTTTTTTAG

Coding sequence (CDS)

ATGGCGACAGATCTTAGTGAATTCACCATCATCAAGGAGGGAGAGGCTGAGATTCTCATGCACGCGAAGAATGAAGTCTTTTTCAATAAGACCCAGGTTAACAACAGAGACATATCCATTGCTGTCCTTAGGGCATTTATATCAAAACGCAAGCAGGAGAATGAAACAAAACTATCAAGAAGAGCAAAATCAGGGCAAAAGATTTCTGAAACTAAAGGTTCTGAAACTGTTATGGAAGCAGCATCTAACGAATCTGCAACAAATGATGAAAAGTGCAATACAGATTGTGATGCTCATGAAAAAACATCAAATGAATGTGGAAGTCCGATGTTGTCAGAAGAACACAAAGAGATCACAGAGGGAAAAGAACAAAGAGATCCCAAGCCTTTTAGAGTTCTTGAGGCCTTGTCAGCTTCTGGGCTAAGAGCCTTAAGATATGCTCGGGAAGTAGAGGGGATTGACAAAGTTGTGGCGCTAGACAATGATAAAGCATCGGTTGAAGCTTGTCGAAGAAACATCAAGTTTAATGGTTCAGTAGCATGTGCGAAAGTGGAATCACATCTTACTGATGCTCGTGTTTATATGCTCACTCACCCAAAAGAATTTGATATGGTTGATCTGGATCCTTACGGTTCACCTTCTGTTTTCTTGGACTCTGCCGTTCAATCAGTTGCTGATGGTGGCATGCTGATGTGCACAGCAACTGATATGGCAGTGCTTTGTGGGGGAAATGGAGAAGTTTGCTATTCAAAATATGGTTCCTATCCTTTGAGGGGGAAATATTGCCACGAAATGGCTTTAAGAATCGTTCTTGCCTGCATTGAGTATGACTATCATCCCTATGAGTGGCTAATGGGTGGGGTTAAAGGCATGGGAAGAAATCTGTGGAAAGATATTGTTTACGAGCTCCTGGCTTTTTTGAGTGTAGTTCATTGTGTTGTGGATGATGGGAGAGATACTTATTTTTGGGAGGATATTTGGGTGGGGGATAGTTCACCCCGCTCCAAGTTTCCTAATCTCTATCAATTGTCCTCCATGAAAAATCGGGTGATTTCTGAGGTTATTACTGTCTTCGGGGGCTCTCCCTCTTATTCTTTGGGTTTCCATCTCTCTTTGACCGATAAGGAAGCGATGAAGGTCGTAGCTCTCGTGTCTTTGATCGGGGAGTTTCAGTTTAGGGATGGGAGGAGGGATATTCGTAGTTGGAGTCTCAGCCCTTCTAAGGGCTTCTCGTGTAGTTCCTTTTTTTAG

Protein sequence

MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQENETKLSRRAKSGQKISETKGSETVMEAASNESATNDEKCNTDCDAHEKTSNECGSPMLSEEHKEITEGKEQRDPKPFRVLEALSASGLRALRYAREVEGIDKVVALDNDKASVEACRRNIKFNGSVACAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVADGGMLMCTATDMAVLCGGNGEVCYSKYGSYPLRGKYCHEMALRIVLACIEYDYHPYEWLMGGVKGMGRNLWKDIVYELLAFLSVVHCVVDDGRDTYFWEDIWVGDSSPRSKFPNLYQLSSMKNRVISEVITVFGGSPSYSLGFHLSLTDKEAMKVVALVSLIGEFQFRDGRRDIRSWSLSPSKGFSCSSFF

Homology

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BLAST of Spg004673 vs. NCBI nr
Match: XP_038890035.1 (probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Benincasa hispida])

HSP 1 Score: 503.1 bits (1294), Expect = 2.4e-138
Identity = 255/282 (90.43%), Postives = 267/282 (94.68%), Query Frame = 0


Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQENETKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR FI+KRKQE+E KLS+
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRLFITKRKQEHEAKLSK 60

Query: 61  RAKSGQKISETKGSETVMEAASNESATNDEKCNTDCDAHEKTSNECGSPMLSEEHKEITE 120
           RAKSGQK++E  GSETVME ASNESATNDEKC+TDC AHEKTSNECGSPMLSEE KE+ E
Sbjct: 61  RAKSGQKVTENNGSETVMEEASNESATNDEKCHTDCGAHEKTSNECGSPMLSEEQKEVAE 120

Query: 121 GKEQRDPKPFRVLEALSASGLRALRYAREVEGIDKVVALDNDKASVEACRRNIKFNGSVA 180
           GKEQRDPKP RVLEALSASGLRALRYAREVEGI +VVALDNDKASVEACRRNIKFNGSVA
Sbjct: 121 GKEQRDPKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVADGGMLMCTATDMAVL 240
           CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPS+FLDSAVQSV DGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRIVLACIEYDYHPYE 283
           CGGNGEVCYSKYGSYPLRGKYCHEMALRI+LACIE   + Y+
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYK 282

[Close]

BLAST of Spg004673 vs. NCBI nr
Match: XP_011656015.1 (probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis sativus] >KGN52527.1 hypothetical protein Csa_008279 [Cucumis sativus])

HSP 1 Score: 494.2 bits (1271), Expect = 1.1e-135
Identity = 250/282 (88.65%), Postives = 264/282 (93.62%), Query Frame = 0


Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQENETKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR+FI+KRKQE+E KLS+
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RAKSGQKISETKGSETVMEAASNESATNDEKCNTDCDAHEKTSNECGSPMLSEEHKEITE 120
           R+KSGQK+SE  GSET  E A NESATNDEKC+TDCDAHEK SNECGSPMLSEE KEI E
Sbjct: 61  RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120

Query: 121 GKEQRDPKPFRVLEALSASGLRALRYAREVEGIDKVVALDNDKASVEACRRNIKFNGSVA 180
           GKEQR PKP RVLEALSASGLRALRYAREVEG+ +VVALDNDKASVEACRRNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVADGGMLMCTATDMAVL 240
           C+KVESHLTDARVYMLTHPKEFDMVDLDPYGSPS+FLDSAVQSV DGGMLMCTATDMAVL
Sbjct: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRIVLACIEYDYHPYE 283
           CGGNGEVCYSKYGSYPLRGKYCHEMALRI+LACIE   + Y+
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYK 282

[Close]

BLAST of Spg004673 vs. NCBI nr
Match: KAG6602539.1 (putative tRNA (guanine(26)-N(2))-dimethyltransferase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 494.2 bits (1271), Expect = 1.1e-135
Identity = 252/282 (89.36%), Postives = 263/282 (93.26%), Query Frame = 0


Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQENETKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLRAFISKRKQE+ETK+SR
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKRKQEHETKISR 60

Query: 61  RAKSGQKISETKGSETVMEAASNESATNDEKCNTDCDAHEKTSNECGSPMLSEEHKEITE 120
           RAKS QKISE  GSE VME ASNESATNDEKCNTDCDAHEK SNECGSP+L EE KE  E
Sbjct: 61  RAKSAQKISENNGSEIVMEEASNESATNDEKCNTDCDAHEKLSNECGSPVLPEEQKENVE 120

Query: 121 GKEQRDPKPFRVLEALSASGLRALRYAREVEGIDKVVALDNDKASVEACRRNIKFNGSVA 180
           GKE+RD KP RVLEALSASGLRALRYAREVEGI +VVALDNDKASVEACRRNIKFNGS+A
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSIA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVADGGMLMCTATDMAVL 240
           CAKVESHLTDARVYMLTHP EFDMVDLDPYGSPS+FLDSAVQSV DGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRIVLACIEYDYHPYE 283
           CGGNGEVCYSKYGSYPLRGKYCHEMALRI+LACIE   + Y+
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYK 282

[Close]

BLAST of Spg004673 vs. NCBI nr
Match: KAG7033216.1 (putative tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 494.2 bits (1271), Expect = 1.1e-135
Identity = 252/282 (89.36%), Postives = 263/282 (93.26%), Query Frame = 0


Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQENETKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLRAFISKRKQE+ETK+SR
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKRKQEHETKISR 60

Query: 61  RAKSGQKISETKGSETVMEAASNESATNDEKCNTDCDAHEKTSNECGSPMLSEEHKEITE 120
           RAKS QKISE  GSE VME ASNESATNDEKCNTDCDAHEK SNECGSP+L EE KE  E
Sbjct: 61  RAKSAQKISENNGSEIVMEEASNESATNDEKCNTDCDAHEKLSNECGSPVLPEEQKENVE 120

Query: 121 GKEQRDPKPFRVLEALSASGLRALRYAREVEGIDKVVALDNDKASVEACRRNIKFNGSVA 180
           GKE+RD KP RVLEALSASGLRALRYAREVEGI +VVALDNDKASVEACRRNIKFNGS+A
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSIA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVADGGMLMCTATDMAVL 240
           CAKVESHLTDARVYMLTHP EFDMVDLDPYGSPS+FLDSAVQSV DGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRIVLACIEYDYHPYE 283
           CGGNGEVCYSKYGSYPLRGKYCHEMALRI+LACIE   + Y+
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYK 282

[Close]

BLAST of Spg004673 vs. NCBI nr
Match: XP_022954530.1 (probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucurbita moschata])

HSP 1 Score: 493.8 bits (1270), Expect = 1.4e-135
Identity = 252/282 (89.36%), Postives = 263/282 (93.26%), Query Frame = 0


Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQENETKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLRAFISKRKQE+ETK+SR
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKRKQEHETKISR 60

Query: 61  RAKSGQKISETKGSETVMEAASNESATNDEKCNTDCDAHEKTSNECGSPMLSEEHKEITE 120
           RAKS QKISE  GSE VME ASNESATNDEKCNTDCDAHEK SNECGSP+LSEE KE   
Sbjct: 61  RAKSAQKISENNGSEIVMEEASNESATNDEKCNTDCDAHEKLSNECGSPVLSEEQKENVA 120

Query: 121 GKEQRDPKPFRVLEALSASGLRALRYAREVEGIDKVVALDNDKASVEACRRNIKFNGSVA 180
           GKE+RD KP RVLEALSASGLRALRYAREVEGI +VVALDNDKASVEACRRNIKFNGS+A
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSIA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVADGGMLMCTATDMAVL 240
           CAKVESHLTDARVYMLTHP EFDMVDLDPYGSPS+FLDSAVQSV DGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRIVLACIEYDYHPYE 283
           CGGNGEVCYSKYGSYPLRGKYCHEMALRI+LACIE   + Y+
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYK 282

[Close]

BLAST of Spg004673 vs. ExPASy Swiss-Prot
Match: Q9SRU7 (Probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=At3g02320 PE=2 SV=3)

HSP 1 Score: 364.8 bits (935), Expect = 1.3e-99
Identity = 190/282 (67.38%), Postives = 225/282 (79.79%), Query Frame = 0


Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQENETKLSR 60
           M TDL+++T+IKEGEAEILMH KN+VFFNK QVNNRD+SIAVLR F+SKRKQE+E K S+
Sbjct: 1   METDLNDYTVIKEGEAEILMHKKNQVFFNKAQVNNRDMSIAVLREFLSKRKQEHEAKSSK 60

Query: 61  RAKSGQKISETKGSETVMEAASNESATNDEKCNTDCDAHEKTSNECGSPMLSEEHKEITE 120
           R +   K+ E   SE   E   +E+  N        +   + ++E G   +S++  + TE
Sbjct: 61  RTRPASKVIEKDASEASKEETPSENGMN--------NGDHEVASEDGPSSVSKDPAKTTE 120

Query: 121 GKEQRDPKPFRVLEALSASGLRALRYAREVEGIDKVVALDNDKASVEACRRNIKFNGSVA 180
               R+PKP +VLEALSASGLRALRYARE+EGI +VVALDND ASVEAC+RNIKFNGSVA
Sbjct: 121 RFAPREPKPPKVLEALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVADGGMLMCTATDMAVL 240
            +KVESH TDARV+MLTHPKEFD+VDLDPYGSPS+FLDSA+QSV DGG+LMCTATDMAVL
Sbjct: 181 ISKVESHHTDARVHMLTHPKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRIVLACIEYDYHPYE 283
           CGGNGEVCYSKYGSYPLR KYCHEMALRI+LA IE   + Y+
Sbjct: 241 CGGNGEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYK 274

[Close]

BLAST of Spg004673 vs. ExPASy Swiss-Prot
Match: Q9LFU5 (Probable tRNA (guanine(26)-N(2))-dimethyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g15810 PE=2 SV=3)

HSP 1 Score: 355.5 bits (911), Expect = 8.0e-97
Identity = 190/282 (67.38%), Postives = 227/282 (80.50%), Query Frame = 0


Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQENETKLSR 60
           M TDL+++T+IKEGEAE+LMH KN+VFFNK QVNNRD+SIAVLRAFI KRKQE+E  LS+
Sbjct: 1   METDLNDYTVIKEGEAEVLMHKKNQVFFNKAQVNNRDMSIAVLRAFIIKRKQEHEAMLSK 60

Query: 61  RAKSGQKISETKGSETVMEAASNESATNDEKCNTDCDAHEKTSNECGSPMLSEEHKEITE 120
           RA+S  K+ E   SET  E    E+  ++ K N +   HE T+ +       +E  +   
Sbjct: 61  RARSSGKVVEKDVSETSKEETPTENGDDNGKTNGE---HEVTTQDG-----PKEAAKTAY 120

Query: 121 GKEQRDPKPFRVLEALSASGLRALRYAREVEGIDKVVALDNDKASVEACRRNIKFNGSVA 180
              +R+ KP RVLEALSASGLRALRYAREVEGI +VVALDND ASVEAC+RNIKFNG ++
Sbjct: 121 ESARRELKPPRVLEALSASGLRALRYAREVEGIGQVVALDNDPASVEACQRNIKFNGLMS 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVADGGMLMCTATDMAVL 240
            +KVESHLTDARV+ML+HPK+FD+VDLDPYG+PS+FLDSAVQSVADGG+LMCTATDMAVL
Sbjct: 181 TSKVESHLTDARVHMLSHPKDFDVVDLDPYGAPSIFLDSAVQSVADGGLLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRIVLACIEYDYHPYE 283
           CG NGEVCYSKYGSYPL+GKYCHEMALRI+LA IE   + Y+
Sbjct: 241 CGANGEVCYSKYGSYPLKGKYCHEMALRILLASIESHANRYK 274

[Close]

BLAST of Spg004673 vs. ExPASy Swiss-Prot
Match: Q3TX08 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Mus musculus OX=10090 GN=Trmt1 PE=1 SV=2)

HSP 1 Score: 166.8 bits (421), Expect = 5.3e-40
Identity = 110/276 (39.86%), Postives = 156/276 (56.52%), Query Frame = 0


Query: 11  IKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQENETKLSRRAKSGQ-KIS 70
           + EG A+I+  + NEVF+N  Q  NRD++ AV+  F          ++   AK  Q K+ 
Sbjct: 58  VTEGAAKIVFPSANEVFYNPVQEFNRDLTCAVITEF---------ARIHLGAKGIQIKVP 117

Query: 71  ETKGSETVMEAASNESATNDEKCNTDCDAHEKTSNECGSPMLSEEHKEITEGKEQRDPKP 130
             K SE +    S              D  E+T+ +  +  L+      T    +   + 
Sbjct: 118 GEKDSEKIAVDLS--------------DQEEETAGK--NENLAPGDWPRTAAVGEICEEG 177

Query: 131 FRVLEALSASGLRALRYAREVEGIDKVVALDNDKASVEACRRNIKFNGSVACAKVESHLT 190
            RVLE L+ASGLR++R+A EV G+  VVA D    +VE   RN++ NG      V+ +  
Sbjct: 178 LRVLEGLAASGLRSIRFALEVPGLQSVVANDASARAVELMHRNVELNG--VAHLVQPNQA 237

Query: 191 DARVYMLTH---PKEFDMVDLDPYGSPSVFLDSAVQSVADGGMLMCTATDMAVLCGGNGE 250
           DAR+ M  H   P+ FD++DLDPYGSP+ FLD+AVQ+V+DGG+L  T TDMAVL G +GE
Sbjct: 238 DARMLMYQHQKAPERFDVIDLDPYGSPAPFLDAAVQAVSDGGLLCVTCTDMAVLAGNSGE 297

Query: 251 VCYSKYGSYPLRGKYCHEMALRIVLACIEYDYHPYE 283
            CYSKYG+  L+ + CHEMALRIVL  ++   + Y+
Sbjct: 298 TCYSKYGAMALKSRACHEMALRIVLHSLDLHANCYQ 306

[Close]

BLAST of Spg004673 vs. ExPASy Swiss-Prot
Match: Q9NXH9 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Homo sapiens OX=9606 GN=TRMT1 PE=1 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 1.4e-37
Identity = 106/277 (38.27%), Postives = 152/277 (54.87%), Query Frame = 0


Query: 9   TIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQENETKLSRRAKSGQKI 68
           T + EG A+I   + NEVF+N  Q  NRD++ AV+  F          ++   AK  Q  
Sbjct: 55  TTVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEF---------ARIQLGAKGIQ-- 114

Query: 69  SETKGSETVMEAASNESATNDEKCNTDCDAHEKTSNECGSPMLSEEHKEITEGKEQRDPK 128
            +  G +   +   + S   +EK     +  E  +   G    +    EI E       +
Sbjct: 115 IKVPGEKDTQKVVVDLSEQEEEK----VELKESENLASGDQPRTAAVGEICE-------E 174

Query: 129 PFRVLEALSASGLRALRYAREVEGIDKVVALDNDKASVEACRRNIKFNGSVACAKVESHL 188
              VLE L+ASGLR++R+A EV G+  VVA D    +V+  RRN++ N       V+   
Sbjct: 175 GLHVLEGLAASGLRSIRFALEVPGLRSVVANDASTRAVDLIRRNVQLND--VAHLVQPSQ 234

Query: 189 TDARVYMLTHPK---EFDMVDLDPYGSPSVFLDSAVQSVADGGMLMCTATDMAVLCGGNG 248
            DAR+ M  H +    FD++DLDPYGSP+ FLD+AVQ+V++GG+L  T TDMAVL G +G
Sbjct: 235 ADARMLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSG 294

Query: 249 EVCYSKYGSYPLRGKYCHEMALRIVLACIEYDYHPYE 283
           E CYSKYG+  L+ + CHEMALRIVL  ++   + Y+
Sbjct: 295 ETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQ 307

[Close]

BLAST of Spg004673 vs. ExPASy Swiss-Prot
Match: Q9VK89 (Probable tRNA (guanine(26)-N(2))-dimethyltransferase OS=Drosophila melanogaster OX=7227 GN=CG6388 PE=2 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 7.1e-37
Identity = 104/273 (38.10%), Postives = 144/273 (52.75%), Query Frame = 0


Query: 10  IIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQENETKLSRRAKSGQKIS 69
           +I+E  AEI+  +   VF+N  Q  NRD+SIA L  +  +  +E   K  +  K  +K+ 
Sbjct: 19  VIRERNAEIV--SGGNVFYNPVQEFNRDLSIAALNVYRQRLTKERSEKALK--KQRKKVK 78

Query: 70  ETKGSETVMEAASNESATNDEKCNTDCDAHEKTSNECGSPMLSEEHKEITEGKEQRDPKP 129
           E +                           EKT+     P + E      +G        
Sbjct: 79  EQE--------------------------DEKTTPVPEDPPVYEAGTRYEDG-------- 138

Query: 130 FRVLEALSASGLRALRYAREVEGIDKVVALDNDKASVEACRRNIKFNGSVACAKVESHLT 189
            R+LEAL+A+GLR++RYA+E+ G+ ++VA D  + +V +   NI+ N      +      
Sbjct: 139 LRILEALAATGLRSIRYAQEIAGVRQIVANDLSRQAVASINTNIRHNKVEELIEPSHSDA 198

Query: 190 DARVYMLTHP-KEFDMVDLDPYGSPSVFLDSAVQSVADGGMLMCTATDMAVLCGGNGEVC 249
              +Y+ T P K FD VDLDPYG P+ FLD A+Q + DGG+L+ TATDMAVL G   E C
Sbjct: 199 MTLMYLSTQPEKRFDAVDLDPYGCPNRFLDGAMQCLVDGGLLLVTATDMAVLAGNAPEAC 253

Query: 250 YSKYGSYPLRGKYCHEMALRIVLACIEYDYHPY 282
           Y KYGS PLR K CHEMALRI+L CIE   + Y
Sbjct: 259 YVKYGSVPLRMKCCHEMALRILLHCIESHANRY 253

[Close]

BLAST of Spg004673 vs. ExPASy TrEMBL
Match: A0A0A0KVU4 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G641600 PE=3 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 5.3e-136
Identity = 250/282 (88.65%), Postives = 264/282 (93.62%), Query Frame = 0


Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQENETKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR+FI+KRKQE+E KLS+
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RAKSGQKISETKGSETVMEAASNESATNDEKCNTDCDAHEKTSNECGSPMLSEEHKEITE 120
           R+KSGQK+SE  GSET  E A NESATNDEKC+TDCDAHEK SNECGSPMLSEE KEI E
Sbjct: 61  RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120

Query: 121 GKEQRDPKPFRVLEALSASGLRALRYAREVEGIDKVVALDNDKASVEACRRNIKFNGSVA 180
           GKEQR PKP RVLEALSASGLRALRYAREVEG+ +VVALDNDKASVEACRRNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVADGGMLMCTATDMAVL 240
           C+KVESHLTDARVYMLTHPKEFDMVDLDPYGSPS+FLDSAVQSV DGGMLMCTATDMAVL
Sbjct: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRIVLACIEYDYHPYE 283
           CGGNGEVCYSKYGSYPLRGKYCHEMALRI+LACIE   + Y+
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYK 282

[Close]

BLAST of Spg004673 vs. ExPASy TrEMBL
Match: A0A6J1GSN6 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111456770 PE=3 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 7.0e-136
Identity = 252/282 (89.36%), Postives = 263/282 (93.26%), Query Frame = 0


Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQENETKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLRAFISKRKQE+ETK+SR
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKRKQEHETKISR 60

Query: 61  RAKSGQKISETKGSETVMEAASNESATNDEKCNTDCDAHEKTSNECGSPMLSEEHKEITE 120
           RAKS QKISE  GSE VME ASNESATNDEKCNTDCDAHEK SNECGSP+LSEE KE   
Sbjct: 61  RAKSAQKISENNGSEIVMEEASNESATNDEKCNTDCDAHEKLSNECGSPVLSEEQKENVA 120

Query: 121 GKEQRDPKPFRVLEALSASGLRALRYAREVEGIDKVVALDNDKASVEACRRNIKFNGSVA 180
           GKE+RD KP RVLEALSASGLRALRYAREVEGI +VVALDNDKASVEACRRNIKFNGS+A
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSIA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVADGGMLMCTATDMAVL 240
           CAKVESHLTDARVYMLTHP EFDMVDLDPYGSPS+FLDSAVQSV DGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRIVLACIEYDYHPYE 283
           CGGNGEVCYSKYGSYPLRGKYCHEMALRI+LACIE   + Y+
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYK 282

[Close]

BLAST of Spg004673 vs. ExPASy TrEMBL
Match: A0A6J1JNF2 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111487439 PE=3 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 1.0e-134
Identity = 251/282 (89.01%), Postives = 261/282 (92.55%), Query Frame = 0


Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQENETKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLRAFISKRKQE+ETK+SR
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKRKQEHETKISR 60

Query: 61  RAKSGQKISETKGSETVMEAASNESATNDEKCNTDCDAHEKTSNECGSPMLSEEHKEITE 120
           RAKS QKISE  GSE VME ASNESATNDEKCNTDCDA EK SNECGSPML E  KE  E
Sbjct: 61  RAKSAQKISENNGSEIVMEEASNESATNDEKCNTDCDAREKLSNECGSPMLPEGQKENVE 120

Query: 121 GKEQRDPKPFRVLEALSASGLRALRYAREVEGIDKVVALDNDKASVEACRRNIKFNGSVA 180
           GKE+RD KP RVLEALSASGLRALRYAREVEGI +VVALDNDKASVEACRRNIKFNGS+A
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSIA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVADGGMLMCTATDMAVL 240
           CAKVESHLTDARVYMLTHP EFDMVDLDPYGSPS+FLDSAVQSV DGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRIVLACIEYDYHPYE 283
           CGGNGEVCYSKYGSYPLRGKYCHEMALRI+LACIE   + Y+
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYK 282

[Close]

BLAST of Spg004673 vs. ExPASy TrEMBL
Match: A0A1S3CA23 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103498546 PE=3 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 5.0e-134
Identity = 247/282 (87.59%), Postives = 262/282 (92.91%), Query Frame = 0


Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQENETKLSR 60
           MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR+FI+KRKQE+E KLS+
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RAKSGQKISETKGSETVMEAASNESATNDEKCNTDCDAHEKTSNECGSPMLSEEHKEITE 120
           R+KSGQK+SE  GSET  E A N SATN+EKC+TDCDAHEK SNECGSPMLSEE KEI E
Sbjct: 61  RSKSGQKLSENNGSETATEEAFNGSATNEEKCHTDCDAHEKPSNECGSPMLSEEQKEIVE 120

Query: 121 GKEQRDPKPFRVLEALSASGLRALRYAREVEGIDKVVALDNDKASVEACRRNIKFNGSVA 180
           GKEQR PKP RVLEALSASGLRALRYAREVEG+ +VVALDNDKASVEAC+RNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACQRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVADGGMLMCTATDMAVL 240
           C KVESHLTDARVYMLTHPKEFDMVDLDPYGSPS+FLDSAVQSV DGGMLMCTATDMAVL
Sbjct: 181 CTKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRIVLACIEYDYHPYE 283
           CGGNGEVCYSKYGSYPLRGKYCHEMALRI+LACIE   + Y+
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYK 282

[Close]

BLAST of Spg004673 vs. ExPASy TrEMBL
Match: A0A5A7T7H3 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002310 PE=3 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 7.2e-133
Identity = 245/282 (86.88%), Postives = 260/282 (92.20%), Query Frame = 0


Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQENETKLSR 60
           M TDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR+FI+KRKQE+E KLS+
Sbjct: 1   MTTDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RAKSGQKISETKGSETVMEAASNESATNDEKCNTDCDAHEKTSNECGSPMLSEEHKEITE 120
           R+KSGQK+SE  GSET  E A N SATN+EKC+TDCDAHEK S ECGSPMLSEE KEI E
Sbjct: 61  RSKSGQKLSENNGSETATEEAFNGSATNEEKCHTDCDAHEKPSTECGSPMLSEEQKEIVE 120

Query: 121 GKEQRDPKPFRVLEALSASGLRALRYAREVEGIDKVVALDNDKASVEACRRNIKFNGSVA 180
           GKEQR PKP RVLEALSASGLRALRYAREVEG+ +VVALDNDKASVEAC+RNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACQRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVADGGMLMCTATDMAVL 240
           C KVESHLTDARVYMLTHPKEFDMVDLDPYGSPS+FLDSAVQSV DGGMLMCTATDMAVL
Sbjct: 181 CTKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRIVLACIEYDYHPYE 283
           CGGNGEVCYSKYGSYPLRGKYCHEMALRI+LACIE   + Y+
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYK 282

[Close]

BLAST of Spg004673 vs. TAIR 10
Match: AT3G02320.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 364.8 bits (935), Expect = 9.4e-101
Identity = 190/282 (67.38%), Postives = 225/282 (79.79%), Query Frame = 0


Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQENETKLSR 60
           M TDL+++T+IKEGEAEILMH KN+VFFNK QVNNRD+SIAVLR F+SKRKQE+E K S+
Sbjct: 1   METDLNDYTVIKEGEAEILMHKKNQVFFNKAQVNNRDMSIAVLREFLSKRKQEHEAKSSK 60

Query: 61  RAKSGQKISETKGSETVMEAASNESATNDEKCNTDCDAHEKTSNECGSPMLSEEHKEITE 120
           R +   K+ E   SE   E   +E+  N        +   + ++E G   +S++  + TE
Sbjct: 61  RTRPASKVIEKDASEASKEETPSENGMN--------NGDHEVASEDGPSSVSKDPAKTTE 120

Query: 121 GKEQRDPKPFRVLEALSASGLRALRYAREVEGIDKVVALDNDKASVEACRRNIKFNGSVA 180
               R+PKP +VLEALSASGLRALRYARE+EGI +VVALDND ASVEAC+RNIKFNGSVA
Sbjct: 121 RFAPREPKPPKVLEALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVADGGMLMCTATDMAVL 240
            +KVESH TDARV+MLTHPKEFD+VDLDPYGSPS+FLDSA+QSV DGG+LMCTATDMAVL
Sbjct: 181 ISKVESHHTDARVHMLTHPKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRIVLACIEYDYHPYE 283
           CGGNGEVCYSKYGSYPLR KYCHEMALRI+LA IE   + Y+
Sbjct: 241 CGGNGEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYK 274

[Close]

BLAST of Spg004673 vs. TAIR 10
Match: AT5G15810.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 355.5 bits (911), Expect = 5.7e-98
Identity = 190/282 (67.38%), Postives = 227/282 (80.50%), Query Frame = 0


Query: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRAFISKRKQENETKLSR 60
           M TDL+++T+IKEGEAE+LMH KN+VFFNK QVNNRD+SIAVLRAFI KRKQE+E  LS+
Sbjct: 99  METDLNDYTVIKEGEAEVLMHKKNQVFFNKAQVNNRDMSIAVLRAFIIKRKQEHEAMLSK 158

Query: 61  RAKSGQKISETKGSETVMEAASNESATNDEKCNTDCDAHEKTSNECGSPMLSEEHKEITE 120
           RA+S  K+ E   SET  E    E+  ++ K N +   HE T+ +       +E  +   
Sbjct: 159 RARSSGKVVEKDVSETSKEETPTENGDDNGKTNGE---HEVTTQDG-----PKEAAKTAY 218

Query: 121 GKEQRDPKPFRVLEALSASGLRALRYAREVEGIDKVVALDNDKASVEACRRNIKFNGSVA 180
              +R+ KP RVLEALSASGLRALRYAREVEGI +VVALDND ASVEAC+RNIKFNG ++
Sbjct: 219 ESARRELKPPRVLEALSASGLRALRYAREVEGIGQVVALDNDPASVEACQRNIKFNGLMS 278

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSVFLDSAVQSVADGGMLMCTATDMAVL 240
            +KVESHLTDARV+ML+HPK+FD+VDLDPYG+PS+FLDSAVQSVADGG+LMCTATDMAVL
Sbjct: 279 TSKVESHLTDARVHMLSHPKDFDVVDLDPYGAPSIFLDSAVQSVADGGLLMCTATDMAVL 338

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRIVLACIEYDYHPYE 283
           CG NGEVCYSKYGSYPL+GKYCHEMALRI+LA IE   + Y+
Sbjct: 339 CGANGEVCYSKYGSYPLKGKYCHEMALRILLASIESHANRYK 372

[Close]

BLAST of Spg004673 vs. TAIR 10
Match: AT3G56330.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 58.5 bits (140), Expect = 1.4e-08
Identity = 50/161 (31.06%), Postives = 78/161 (48.45%), Query Frame = 0


Query: 124 QRDPKPFRVLEALSASGLRALRYAREVEGIDKVVALDNDKASVEACRRNIKFNGSVACAK 183
           +R     RVL+A+   G+R+LRY  E E  D V+A D +  +    RR I  N     +K
Sbjct: 84  KRSNGSLRVLDAMCGCGIRSLRYLVEAEA-DFVMANDANDDN----RRVITDN----LSK 143

Query: 184 VESHLTDARVYMLTH-------------PKEFDMVDLDPYGSPSVFLDSAVQSVADGGML 243
           VE    D R +++TH                FDM+D+D +GS S FL  A  ++  GG+L
Sbjct: 144 VERGTGDERRWVVTHMLANKAMIERYMVADFFDMIDIDSFGSDSSFLRDAFNALRLGGLL 203

Query: 244 MCTATDMAVLCGGNGEVCYSKYGSYPLRGKYCHEMALRIVL 272
             T+TD     G       + YG++     + +E+ LR+++
Sbjct: 204 YLTSTDGYSSGGHRPYNSLAAYGAFIRPMPFGNEIGLRMLI 235

The following BLAST results are available for this feature:

BLAST of Spg004673 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038890035.1	2.4e-138	90.43	probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Benincasa hispida]	[more]
XP_011656015.1	1.1e-135	88.65	probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis sativus] >KGN525...	[more]
KAG6602539.1	1.1e-135	89.36	putative tRNA (guanine(26)-N(2))-dimethyltransferase 2, partial [Cucurbita argyr...	[more]
KAG7033216.1	1.1e-135	89.36	putative tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucurbita argyrosperma s...	[more]
XP_022954530.1	1.4e-135	89.36	probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucurbita moschata]	[more]

BLAST of Spg004673 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SRU7	1.3e-99	67.38	Probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 OS=Arabidopsis thaliana O...	[more]
Q9LFU5	8.0e-97	67.38	Probable tRNA (guanine(26)-N(2))-dimethyltransferase 1 OS=Arabidopsis thaliana O...	[more]
Q3TX08	5.3e-40	39.86	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Mus musculus OX=10090 GN=Trmt1 PE...	[more]
Q9NXH9	1.4e-37	38.27	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Homo sapiens OX=9606 GN=TRMT1 PE=...	[more]
Q9VK89	7.1e-37	38.10	Probable tRNA (guanine(26)-N(2))-dimethyltransferase OS=Drosophila melanogaster ...	[more]

BLAST of Spg004673 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KVU4	5.3e-136	88.65	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G...	[more]
A0A6J1GSN6	7.0e-136	89.36	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1JNF2	1.0e-134	89.01	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A1S3CA23	5.0e-134	87.59	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103498...	[more]
A0A5A7T7H3	7.2e-133	86.88	tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=11946...	[more]

BLAST of Spg004673 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G02320.1	9.4e-101	67.38	N2,N2-dimethylguanosine tRNA methyltransferase	[more]
AT5G15810.1	5.7e-98	67.38	N2,N2-dimethylguanosine tRNA methyltransferase	[more]
AT3G56330.1	1.4e-08	31.06	N2,N2-dimethylguanosine tRNA methyltransferase	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002905	tRNA methyltransferase, Trm1	PFAM	PF02005	TRM	coord: 12..283 e-value: 1.2E-63 score: 215.5
IPR002905	tRNA methyltransferase, Trm1	PANTHER	PTHR10631	N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE	coord: 1..282
IPR002905	tRNA methyltransferase, Trm1	PROSITE	PS51626	SAM_MT_TRM1	coord: 9..275 score: 70.180229
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 8..284 e-value: 9.2E-77 score: 259.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 53..92
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 53..68
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 69..89
None	No IPR available	PANTHER	PTHR10631:SF12	TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE 1-RELATED	coord: 1..282
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 8..285

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg004673.1	Spg004673.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0002940	tRNA N2-guanine methylation
biological_process	GO:0008033	tRNA processing
cellular_component	GO:0005634	nucleus
molecular_function	GO:0000049	tRNA binding
molecular_function	GO:0004809	tRNA (guanine-N2-)-methyltransferase activity
molecular_function	GO:0003723	RNA binding