Spg004499 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg004499
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionThioredoxin-like protein
Locationscaffold9: 13181007 .. 13183938 (-)
RNA-Seq ExpressionSpg004499
SyntenySpg004499
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTAGATTTTTCCCCTTCGCTTTGGCATCTCCGATAAGCCAAAACAGACGACAGAATTCCTCTATCGACATTGTTGGAGCAGTGAGCGCTTCACATGGCGGCATCCATTTCAACTTCGGCTTATCCTCTCCTCACCTCAGAGCGCTCCAACCGCTTGGTTGCTAATGATTCATCTTTGTCGAGGTTGTCGTCCACCTCGTCTCTACAGCTACCAGTTCAGCTTCGGTGCGTTCGACTCGGAAACAATGGGGTTTCAGCTCCGTCGCGGTCTCGAATCTTGCCTGTGGTAATAATCTCTTTTCTTAGATCTGTATGTCGTTGTAATGGAGGAACCTGATAAAGAAATTGCAATATATGGACTTTCCTTTTGTTCTCATTTTTTATTTTCTTCGAATCTAATTTTCAGCGAATTATTGTTAATTTTTACACAGAACTTGAAACTACACGGTTCGATTAGAATTTTACCTTAGAAAAAAGTATACTCTATTTGGTTCTATTACTTTTAGGTTTATCCGAAAATTCAGTTCTCAAGTAGATTTCCCGAAGTACTTGTTCATTCCGAATGATTGGAAAGATTGCATTTCCATTTTCTTCAAGACGTCCTGCTCCTTGCTCTTAACATCACAACTGTTGCATTAGTATTTTTTGTTATTCTTAAGTAGCTTCTTTGAATATGGAAATATGTGTTCAAACTATTGTAATGAATAACGTGGACTTGTTTGTACATAACTTCTGTAGGTCAAAGCCAAGAATCAAACATTTTCCTCCTTTGATGATTTGCTGGCCAACTCTGACAAACCTGTGTTAGTCGACTTCTATGCTACCTGGTCAGTTACTCTCTCTCTCGCTCTTACATGCATGCACGCGTCACGTACCTATGTTCAAAGCACTCTTTCTAGAAGAGCATTGCAGGCGTGACATGATATCTCATCACACTTTATTCGCACAATGTTATATGTGGAGTCACTTTGTGTCTAATAGATCTATTAGACACTTTTCTAAGTTTAGGAACCTAACTTATAATTTAATCTAAAATTTAAAATTTGCAGACTAGTTTGGTTGGTTTAGTCAATTGTGTCAATTCTGGCCTCAATTTGGATTGAAATGTGCCAATTGAATCTGCTTATTTGCACATTGCCTCACCAAAGGGGTTATGTCCTTTACATTTTTTAGCTCATTTCTGTTTAGAAATTCAGCATTGTAAATGATTTGAAGCACATATTGTTTATGTTTGATTATATACTTAAAAGATATCTCAACAATGTCTAATTTCATATTTAAAAGTTTTTTTTTATACATGCTTGTTGAAGCGTTCTTGGATGTTTGTCCAAGATTTGAGTTGCACCACACCTATTTTTAAAGTACTCCTAGAAAAGCATTGCACGCGTGGTATGATACCCATCATACTTTCTTCACACAATGGTAATCCGTGGAGACATTTTTAATCTACTCTTTCAAGACAAGCCATTGTTACTCGGGAGCAATTTTACCATGTGAATATGATTCAATGGGCGAGTTAGTGCTTGAAGGTTGTGGAGAAAATGAGTCAGCAGGGGTTTAATTGTAGAATGGAGTATTTCCTTTTAGAAAAAAGTAATGTAACGTTTTTAAATGATGAGTAAACCTAGCTCCTCAATTGCAGGTGTGGTCCTTGTCAGTTCATGGTTCCCATTCTTGAACAAGTTAGTGCTACACTGAGTGACACAGTCCAGGTGGTCAAGATTGACACAGAGAAATATCCTGTCATTGCTGATAAATACAGAATAGAGGCATTGCCTACATTCATCCTCTTCAAAGATGGAAAACCCCTTGATCGATTCGTAAGTAAAAGCCTTGTCTCATTTCTAGTCATTTGGAGATATATCGAATGTACAACTTATTGTAAGCTTTGTTCAGTCAAATGCGTGAAGATATCTCATATATGATATATGTTTCAGAATCTTATTTAGTTACAATTTTTGTCTTTTCTGAAGTTGAATGAAAATGGGAAGTTTAACCAATTAGTTGTGTCAAGAAAATCTGTAATTACGTTAACAGTTGAAGTTTTTATAAAGATATATCCTTTATTGCCATTAAACCATTGTCTACTGTATCTTTACTCTATTCGTGCAGCCAGTTTAATTTCAAAGAGTTAAAGGTATTCCCATATTTTCAATGTTTAACCCTTTAAAACACATTAGCACATCCTCTTTCCTACTAGACTACAACTATTTTGCCTTTTCTTTTCGTTTTAGTGAGATCTCATAAACAATCTTTAACTTTTTAGACATTCCTGTTGAAATGTCAGTTAATCGCACTTTCAGTTTATTTTGCTTCGATTATTAATTATTTATTTTAAACATGCATGCAAATGATAAATGTAGGAAGGAGCTTTGACTGCGGTTGAACTCGTCAAACGCATTGAAGATTCCCTGAAGGTCAAGCAATAACACTGGTGAGACTCTTACTTTGATTGAAGAATTTATCTGAACTTGCATGTTTAGAAGATTTAAGAGCCCATATCAAATCAACAGAAAATTCAACCTCAGTTTGGAGGCAAACTTGGTATTACTAAAAAACCAGCTCAAACCAAAATGGAAGAAAGACAAGGGAAAAGAAGGCAAGCCTCTTATAGACATTTTATAGTTTTAATCTGTCAGAAAGGCATCAAGAAAATTGCACACTGTAACCATCTCTTCAATTCTCATTTTATGGTGTGTTTATAACACTACCATATCCCCAACTGCTATTTTCAATAAACTTAGTGGCTTTATAGAAAATAATGCACTTGCAAATAATATTTGGGATTTATAAGTGCACATCTGTTGGTATTAATTTTGGCATAAATTTGATATCGAAAATTGACCATGTGGTTTTGGTTAGTTGAAAACTGACAGTTTCTTAAGCTTGTACAATCGATTGATCATCTTTTGCTTCAATTGGTTGATTTTGGC

mRNA sequence

ATGGCGGCATCCATTTCAACTTCGGCTTATCCTCTCCTCACCTCAGAGCGCTCCAACCGCTTGGTTGCTAATGATTCATCTTTGTCGAGGTTGTCGTCCACCTCGTCTCTACAGCTACCAGTTCAGCTTCGGTGCGTTCGACTCGGAAACAATGGGGTTTCAGCTCCGTCGCGGTCTCGAATCTTGCCTGTGGTCAAAGCCAAGAATCAAACATTTTCCTCCTTTGATGATTTGCTGGCCAACTCTGACAAACCTGTGTTAGTCGACTTCTATGCTACCTGGTGTGGTCCTTGTCAGTTCATGGTTCCCATTCTTGAACAAGTTAGTGCTACACTGAGTGACACAGTCCAGGTGGTCAAGATTGACACAGAGAAATATCCTGTCATTGCTGATAAATACAGAATAGAGGCATTGCCTACATTCATCCTCTTCAAAGATGGAAAACCCCTTGATCGATTCGAAGGAGCTTTGACTGCGGTTGAACTCGTCAAACGCATTGAAGATTCCCTGAAGGTCAAGCAATAA

Coding sequence (CDS)

ATGGCGGCATCCATTTCAACTTCGGCTTATCCTCTCCTCACCTCAGAGCGCTCCAACCGCTTGGTTGCTAATGATTCATCTTTGTCGAGGTTGTCGTCCACCTCGTCTCTACAGCTACCAGTTCAGCTTCGGTGCGTTCGACTCGGAAACAATGGGGTTTCAGCTCCGTCGCGGTCTCGAATCTTGCCTGTGGTCAAAGCCAAGAATCAAACATTTTCCTCCTTTGATGATTTGCTGGCCAACTCTGACAAACCTGTGTTAGTCGACTTCTATGCTACCTGGTGTGGTCCTTGTCAGTTCATGGTTCCCATTCTTGAACAAGTTAGTGCTACACTGAGTGACACAGTCCAGGTGGTCAAGATTGACACAGAGAAATATCCTGTCATTGCTGATAAATACAGAATAGAGGCATTGCCTACATTCATCCTCTTCAAAGATGGAAAACCCCTTGATCGATTCGAAGGAGCTTTGACTGCGGTTGAACTCGTCAAACGCATTGAAGATTCCCTGAAGGTCAAGCAATAA

Protein sequence

MAASISTSAYPLLTSERSNRLVANDSSLSRLSSTSSLQLPVQLRCVRLGNNGVSAPSRSRILPVVKAKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILEQVSATLSDTVQVVKIDTEKYPVIADKYRIEALPTFILFKDGKPLDRFEGALTAVELVKRIEDSLKVKQ
Homology
BLAST of Spg004499 vs. NCBI nr
Match: XP_022974037.1 (thioredoxin Y1, chloroplastic-like [Cucurbita maxima] >XP_022974038.1 thioredoxin Y1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 301.6 bits (771), Expect = 4.4e-78
Identity = 156/174 (89.66%), Postives = 163/174 (93.68%), Query Frame = 0

Query: 1   MAASISTSAYPLLTSERSNRLVANDSSLSRLSSTSSLQLPVQLRCVRLGNNGVSAPSRSR 60
           MAASISTS YPLLTSERSNR  ANDSS SRL S+SSLQLPVQLRCVRLGN G+SAPSRSR
Sbjct: 1   MAASISTSPYPLLTSERSNRFAANDSSSSRLFSSSSLQLPVQLRCVRLGNCGISAPSRSR 60

Query: 61  ILPVVKAKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILEQVSATLSDTVQVVK 120
           I PVVKAKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILE+VS  LSDTVQVVK
Sbjct: 61  IFPVVKAKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILEKVSDALSDTVQVVK 120

Query: 121 IDTEKYPVIADKYRIEALPTFILFKDGKPLDRFEGALTAVELVKRIEDSLKVKQ 175
           IDTEKYPVIADKY+IEALPTFILFKDGKPLDRFEGAL A EL++RIE+SLKVKQ
Sbjct: 121 IDTEKYPVIADKYKIEALPTFILFKDGKPLDRFEGALAAGELIQRIENSLKVKQ 174

BLAST of Spg004499 vs. NCBI nr
Match: XP_022972902.1 (uncharacterized protein LOC111471411 [Cucurbita maxima])

HSP 1 Score: 298.5 bits (763), Expect = 3.7e-77
Identity = 154/174 (88.51%), Postives = 164/174 (94.25%), Query Frame = 0

Query: 1   MAASISTSAYPLLTSERSNRLVANDSSLSRLSSTSSLQLPVQLRCVRLGNNGVSAPSRSR 60
           MAASISTSA PLLTSER NRL AN +SLSRLSSTSS+QLPVQLRCVRLGN+GVS  SRSR
Sbjct: 1   MAASISTSANPLLTSERPNRLAANVTSLSRLSSTSSIQLPVQLRCVRLGNHGVSTLSRSR 60

Query: 61  ILPVVKAKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILEQVSATLSDTVQVVK 120
           ILPV+KA NQTFSSFDDLLANS KPVLVDFYATWCGPCQFMVPILE+VSA LSDTVQVVK
Sbjct: 61  ILPVIKASNQTFSSFDDLLANSGKPVLVDFYATWCGPCQFMVPILEKVSAKLSDTVQVVK 120

Query: 121 IDTEKYPVIADKYRIEALPTFILFKDGKPLDRFEGALTAVELVKRIEDSLKVKQ 175
           IDTEKYPVIADKY+IEALPTF+LFKDGKPLDRFEGA+TA +L+KRIEDSLKVKQ
Sbjct: 121 IDTEKYPVIADKYKIEALPTFVLFKDGKPLDRFEGAMTADDLIKRIEDSLKVKQ 174

BLAST of Spg004499 vs. NCBI nr
Match: XP_023520827.1 (uncharacterized protein LOC111784322 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023520828.1 uncharacterized protein LOC111784322 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023520939.1 uncharacterized protein LOC111784479 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 297.4 bits (760), Expect = 8.3e-77
Identity = 155/174 (89.08%), Postives = 161/174 (92.53%), Query Frame = 0

Query: 1   MAASISTSAYPLLTSERSNRLVANDSSLSRLSSTSSLQLPVQLRCVRLGNNGVSAPSRSR 60
           MAASISTS YPLLTSERSNR   NDSS SRL S+SSLQLPVQLRCVRLGN G+SA SRSR
Sbjct: 1   MAASISTSPYPLLTSERSNRFAVNDSSSSRLFSSSSLQLPVQLRCVRLGNCGISAQSRSR 60

Query: 61  ILPVVKAKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILEQVSATLSDTVQVVK 120
           I PVVKAKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILE+VS  LSDTVQVVK
Sbjct: 61  IFPVVKAKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILEKVSDALSDTVQVVK 120

Query: 121 IDTEKYPVIADKYRIEALPTFILFKDGKPLDRFEGALTAVELVKRIEDSLKVKQ 175
           IDTEKYPVIADKY+IEALPTFILFKDGKPLDRFEGAL A ELV+RIE+SLKVKQ
Sbjct: 121 IDTEKYPVIADKYKIEALPTFILFKDGKPLDRFEGALAAGELVQRIENSLKVKQ 174

BLAST of Spg004499 vs. NCBI nr
Match: XP_022932802.1 (thioredoxin Y2, chloroplastic [Cucurbita moschata])

HSP 1 Score: 296.2 bits (757), Expect = 1.8e-76
Identity = 152/174 (87.36%), Postives = 161/174 (92.53%), Query Frame = 0

Query: 1   MAASISTSAYPLLTSERSNRLVANDSSLSRLSSTSSLQLPVQLRCVRLGNNGVSAPSRSR 60
           MAASISTS YPLLTSERSNR   NDSS SRL  +SSLQLPVQLRCVRLGN G+SAPSRSR
Sbjct: 1   MAASISTSPYPLLTSERSNRFAVNDSSSSRLFFSSSLQLPVQLRCVRLGNCGISAPSRSR 60

Query: 61  ILPVVKAKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILEQVSATLSDTVQVVK 120
           I PVVKAKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILE+VS  LSDTVQVVK
Sbjct: 61  IFPVVKAKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILEKVSDALSDTVQVVK 120

Query: 121 IDTEKYPVIADKYRIEALPTFILFKDGKPLDRFEGALTAVELVKRIEDSLKVKQ 175
           IDTEKYPVIADKY+IEALPTFILFKDGKPLDRFEGA+ A EL++RIE+SLK+KQ
Sbjct: 121 IDTEKYPVIADKYKIEALPTFILFKDGKPLDRFEGAVAAGELIQRIENSLKIKQ 174

BLAST of Spg004499 vs. NCBI nr
Match: XP_022932509.1 (uncharacterized protein LOC111438908 [Cucurbita moschata] >KAG6607564.1 Thioredoxin Y1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037199.1 Thioredoxin Y1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 294.3 bits (752), Expect = 7.0e-76
Identity = 154/174 (88.51%), Postives = 162/174 (93.10%), Query Frame = 0

Query: 1   MAASISTSAYPLLTSERSNRLVANDSSLSRLSSTSSLQLPVQLRCVRLGNNGVSAPSRSR 60
           MAASISTSA PLLTSER N L AN +SLSRLSSTSS+QLPVQLRCVRLGN+GVS  SRSR
Sbjct: 1   MAASISTSANPLLTSERPNLLAANVTSLSRLSSTSSIQLPVQLRCVRLGNHGVSTLSRSR 60

Query: 61  ILPVVKAKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILEQVSATLSDTVQVVK 120
           ILPVVKA NQTFSSFDDLLANS KPVLVDFYATWCGPCQFMVPILE+VSA LSDTVQVVK
Sbjct: 61  ILPVVKASNQTFSSFDDLLANSGKPVLVDFYATWCGPCQFMVPILEKVSAKLSDTVQVVK 120

Query: 121 IDTEKYPVIADKYRIEALPTFILFKDGKPLDRFEGALTAVELVKRIEDSLKVKQ 175
           IDTEKYPVIADKY+IEALPTFILFKDGKP DRFEGA+TA +L+KRIEDSLKVKQ
Sbjct: 121 IDTEKYPVIADKYKIEALPTFILFKDGKPRDRFEGAMTADDLIKRIEDSLKVKQ 174

BLAST of Spg004499 vs. ExPASy Swiss-Prot
Match: Q6NPF9 (Thioredoxin Y1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g76760 PE=1 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 3.8e-48
Identity = 109/172 (63.37%), Postives = 128/172 (74.42%), Query Frame = 0

Query: 4   SISTSAYPLLTSERSNRLVANDSSLSRLSSTSSLQLPVQLRCVRLGN-NGVSAPSRSRIL 63
           S+S+S  P L S+ S+ + A     SR  S    Q PV  R VR G+    S  S +R  
Sbjct: 5   SLSSSTVPSLNSKESSGVSA---FASRSISAVKFQFPV--RRVRTGDLKFPSLSSTTRCT 64

Query: 64  P-VVKAKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILEQVSATLSDTVQVVKI 123
           P  ++AK QTF SF+DLL NSDKPVLVD+YATWCGPCQFMVPIL +VS TL D +QVVKI
Sbjct: 65  PRRIEAKKQTFDSFEDLLVNSDKPVLVDYYATWCGPCQFMVPILNEVSETLKDKIQVVKI 124

Query: 124 DTEKYPVIADKYRIEALPTFILFKDGKPLDRFEGALTAVELVKRIEDSLKVK 174
           DTEKYP IA+KY+IEALPTFILFKDG+P DRFEGALTA +L++RIEDSLKVK
Sbjct: 125 DTEKYPSIANKYKIEALPTFILFKDGEPCDRFEGALTAKQLIQRIEDSLKVK 171

BLAST of Spg004499 vs. ExPASy Swiss-Prot
Match: Q8L7S9 (Thioredoxin Y2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g43560 PE=2 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 7.9e-46
Identity = 108/176 (61.36%), Postives = 128/176 (72.73%), Query Frame = 0

Query: 1   MAASISTS-AYPLLTSERSNRLVANDSSLSRLSSTSSLQLPVQLRCVRLGN-NGVSAPSR 60
           MA S++T+   P  T E SN       S S   + SS++LP Q+R  R G+    S+ +R
Sbjct: 1   MAISLATAYISPCFTPESSN-------SASPSRTLSSVRLPSQIR--RFGSVQSPSSSTR 60

Query: 61  SRILPVVKAKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILEQVSATLSDTVQV 120
              L V  AK QTF+SFDDLL NSDKPVLVDFYATWCGPCQ MVPIL +VS TL D + V
Sbjct: 61  FAPLTVRAAKKQTFNSFDDLLQNSDKPVLVDFYATWCGPCQLMVPILNEVSETLKDIIAV 120

Query: 121 VKIDTEKYPVIADKYRIEALPTFILFKDGKPLDRFEGALTAVELVKRIEDSLKVKQ 175
           VKIDTEKYP +A+KY+IEALPTFILFKDGK  DRFEGAL A +LV+RIE+SL+VKQ
Sbjct: 121 VKIDTEKYPSLANKYQIEALPTFILFKDGKLWDRFEGALPANQLVERIENSLQVKQ 167

BLAST of Spg004499 vs. ExPASy Swiss-Prot
Match: Q5JMR9 (Thioredoxin Y, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0963400 PE=3 SV=2)

HSP 1 Score: 164.9 bits (416), Expect = 8.4e-40
Identity = 94/169 (55.62%), Postives = 117/169 (69.23%), Query Frame = 0

Query: 4   SISTSAYPLLTSERSNRLVANDSSLSRLSSTSSLQLPVQLRCVRLGNNGVSAPSRSRILP 63
           S +T+A    T  R   LVA  S+    S+      PV    V  G    +APSR     
Sbjct: 6   STTTAAAASPTPCRPAALVARSSAAPLRSAA-----PV---VVAAGLRRAAAPSRRGATL 65

Query: 64  VVKAKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILEQVSATLSDTVQVVKIDT 123
            V+AK QTFSSFD+LL  S+KPVLVDFYATWCGPCQ+MVPIL++VS  L D +QVVKIDT
Sbjct: 66  RVQAKKQTFSSFDELLEKSEKPVLVDFYATWCGPCQYMVPILQEVSEKLGDKIQVVKIDT 125

Query: 124 EKYPVIADKYRIEALPTFILFKDGKPLDRFEGALTAVELVKRIEDSLKV 173
           EKY  IA++Y+IEALPTFI+FK+GKP  RFEGAL A +L+++IE +L+V
Sbjct: 126 EKYTSIANRYQIEALPTFIIFKNGKPCHRFEGALPANQLIQQIESALEV 166

BLAST of Spg004499 vs. ExPASy Swiss-Prot
Match: P52232 (Thioredoxin-like protein slr0233 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr0233 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 3.9e-29
Identity = 57/101 (56.44%), Postives = 78/101 (77.23%), Query Frame = 0

Query: 67  AKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILEQVSATLSDTVQVVKIDTEKY 126
           A  + F++F ++LA S KPVLVDFYATWCGPCQ M PILEQV + L   +QVVKIDT+KY
Sbjct: 2   AVKKQFANFAEMLAGSPKPVLVDFYATWCGPCQMMAPILEQVGSHLRQQIQVVKIDTDKY 61

Query: 127 PVIADKYRIEALPTFILFKDGKPLDRFEGALTAVELVKRIE 168
           P IA +Y+I++LPT +LFK G+P+ R EG   A +L+++++
Sbjct: 62  PAIATQYQIQSLPTLVLFKQGQPVHRMEGVQQAAQLIQQLQ 102

BLAST of Spg004499 vs. ExPASy Swiss-Prot
Match: O30974 (Thioredoxin OS=Mycolicibacterium smegmatis OX=1772 GN=trxA PE=1 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 3.1e-18
Identity = 44/102 (43.14%), Postives = 64/102 (62.75%), Query Frame = 0

Query: 71  TFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILEQVSATLSDTVQVVKI--DTEKYPV 130
           T  SF   +  S KPVLVDF+ATWCGPC+ + P+LE+++A   D + V KI  D +  P 
Sbjct: 11  TDDSFSTDVLGSSKPVLVDFWATWCGPCKMVAPVLEEIAAEKGDQLTVAKIDVDVDANPA 70

Query: 131 IADKYRIEALPTFILFKDGKPLDRFEGALTAVELVKRIEDSL 171
            A  +++ ++PT ILFKDG P+ R  GA     L++ + D+L
Sbjct: 71  TARDFQVVSIPTMILFKDGAPVKRIVGAKGKAALLRELSDAL 112

BLAST of Spg004499 vs. ExPASy TrEMBL
Match: A0A6J1I964 (thioredoxin Y1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111472651 PE=4 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 2.1e-78
Identity = 156/174 (89.66%), Postives = 163/174 (93.68%), Query Frame = 0

Query: 1   MAASISTSAYPLLTSERSNRLVANDSSLSRLSSTSSLQLPVQLRCVRLGNNGVSAPSRSR 60
           MAASISTS YPLLTSERSNR  ANDSS SRL S+SSLQLPVQLRCVRLGN G+SAPSRSR
Sbjct: 1   MAASISTSPYPLLTSERSNRFAANDSSSSRLFSSSSLQLPVQLRCVRLGNCGISAPSRSR 60

Query: 61  ILPVVKAKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILEQVSATLSDTVQVVK 120
           I PVVKAKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILE+VS  LSDTVQVVK
Sbjct: 61  IFPVVKAKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILEKVSDALSDTVQVVK 120

Query: 121 IDTEKYPVIADKYRIEALPTFILFKDGKPLDRFEGALTAVELVKRIEDSLKVKQ 175
           IDTEKYPVIADKY+IEALPTFILFKDGKPLDRFEGAL A EL++RIE+SLKVKQ
Sbjct: 121 IDTEKYPVIADKYKIEALPTFILFKDGKPLDRFEGALAAGELIQRIENSLKVKQ 174

BLAST of Spg004499 vs. ExPASy TrEMBL
Match: A0A6J1I628 (uncharacterized protein LOC111471411 OS=Cucurbita maxima OX=3661 GN=LOC111471411 PE=4 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 1.8e-77
Identity = 154/174 (88.51%), Postives = 164/174 (94.25%), Query Frame = 0

Query: 1   MAASISTSAYPLLTSERSNRLVANDSSLSRLSSTSSLQLPVQLRCVRLGNNGVSAPSRSR 60
           MAASISTSA PLLTSER NRL AN +SLSRLSSTSS+QLPVQLRCVRLGN+GVS  SRSR
Sbjct: 1   MAASISTSANPLLTSERPNRLAANVTSLSRLSSTSSIQLPVQLRCVRLGNHGVSTLSRSR 60

Query: 61  ILPVVKAKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILEQVSATLSDTVQVVK 120
           ILPV+KA NQTFSSFDDLLANS KPVLVDFYATWCGPCQFMVPILE+VSA LSDTVQVVK
Sbjct: 61  ILPVIKASNQTFSSFDDLLANSGKPVLVDFYATWCGPCQFMVPILEKVSAKLSDTVQVVK 120

Query: 121 IDTEKYPVIADKYRIEALPTFILFKDGKPLDRFEGALTAVELVKRIEDSLKVKQ 175
           IDTEKYPVIADKY+IEALPTF+LFKDGKPLDRFEGA+TA +L+KRIEDSLKVKQ
Sbjct: 121 IDTEKYPVIADKYKIEALPTFVLFKDGKPLDRFEGAMTADDLIKRIEDSLKVKQ 174

BLAST of Spg004499 vs. ExPASy TrEMBL
Match: A0A6J1EXS8 (thioredoxin Y2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111439262 PE=4 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 8.9e-77
Identity = 152/174 (87.36%), Postives = 161/174 (92.53%), Query Frame = 0

Query: 1   MAASISTSAYPLLTSERSNRLVANDSSLSRLSSTSSLQLPVQLRCVRLGNNGVSAPSRSR 60
           MAASISTS YPLLTSERSNR   NDSS SRL  +SSLQLPVQLRCVRLGN G+SAPSRSR
Sbjct: 1   MAASISTSPYPLLTSERSNRFAVNDSSSSRLFFSSSLQLPVQLRCVRLGNCGISAPSRSR 60

Query: 61  ILPVVKAKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILEQVSATLSDTVQVVK 120
           I PVVKAKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILE+VS  LSDTVQVVK
Sbjct: 61  IFPVVKAKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILEKVSDALSDTVQVVK 120

Query: 121 IDTEKYPVIADKYRIEALPTFILFKDGKPLDRFEGALTAVELVKRIEDSLKVKQ 175
           IDTEKYPVIADKY+IEALPTFILFKDGKPLDRFEGA+ A EL++RIE+SLK+KQ
Sbjct: 121 IDTEKYPVIADKYKIEALPTFILFKDGKPLDRFEGAVAAGELIQRIENSLKIKQ 174

BLAST of Spg004499 vs. ExPASy TrEMBL
Match: A0A6J1EX66 (uncharacterized protein LOC111438908 OS=Cucurbita moschata OX=3662 GN=LOC111438908 PE=4 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 3.4e-76
Identity = 154/174 (88.51%), Postives = 162/174 (93.10%), Query Frame = 0

Query: 1   MAASISTSAYPLLTSERSNRLVANDSSLSRLSSTSSLQLPVQLRCVRLGNNGVSAPSRSR 60
           MAASISTSA PLLTSER N L AN +SLSRLSSTSS+QLPVQLRCVRLGN+GVS  SRSR
Sbjct: 1   MAASISTSANPLLTSERPNLLAANVTSLSRLSSTSSIQLPVQLRCVRLGNHGVSTLSRSR 60

Query: 61  ILPVVKAKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILEQVSATLSDTVQVVK 120
           ILPVVKA NQTFSSFDDLLANS KPVLVDFYATWCGPCQFMVPILE+VSA LSDTVQVVK
Sbjct: 61  ILPVVKASNQTFSSFDDLLANSGKPVLVDFYATWCGPCQFMVPILEKVSAKLSDTVQVVK 120

Query: 121 IDTEKYPVIADKYRIEALPTFILFKDGKPLDRFEGALTAVELVKRIEDSLKVKQ 175
           IDTEKYPVIADKY+IEALPTFILFKDGKP DRFEGA+TA +L+KRIEDSLKVKQ
Sbjct: 121 IDTEKYPVIADKYKIEALPTFILFKDGKPRDRFEGAMTADDLIKRIEDSLKVKQ 174

BLAST of Spg004499 vs. ExPASy TrEMBL
Match: A0A6J1DH52 (thioredoxin Y2, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111020017 PE=4 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 9.9e-76
Identity = 153/175 (87.43%), Postives = 160/175 (91.43%), Query Frame = 0

Query: 1   MAASISTSAYPLLTSERSNRLVAND-SSLSRLSSTSSLQLPVQLRCVRLGNNGVSAPSRS 60
           MAASISTS YPLLTSERSNR  AND SS SR SS+SSL LP Q RC RLGNNG+SAPSRS
Sbjct: 1   MAASISTSPYPLLTSERSNRFPANDSSSSSRFSSSSSLHLPPQFRCFRLGNNGISAPSRS 60

Query: 61  RILPVVKAKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILEQVSATLSDTVQVV 120
           RILPVVKAKNQTFSSFDDLLANSDKPV VDFYATWCGPCQFMVPILE+VSATL DTVQVV
Sbjct: 61  RILPVVKAKNQTFSSFDDLLANSDKPVFVDFYATWCGPCQFMVPILEKVSATLGDTVQVV 120

Query: 121 KIDTEKYPVIADKYRIEALPTFILFKDGKPLDRFEGALTAVELVKRIEDSLKVKQ 175
           KIDTEKYP IADKY+IEALPTFILFKDGK LDRFEGALTA +L++RIEDSLKVKQ
Sbjct: 121 KIDTEKYPAIADKYKIEALPTFILFKDGKLLDRFEGALTARDLIQRIEDSLKVKQ 175

BLAST of Spg004499 vs. TAIR 10
Match: AT1G76760.1 (thioredoxin Y1 )

HSP 1 Score: 192.6 bits (488), Expect = 2.7e-49
Identity = 109/172 (63.37%), Postives = 128/172 (74.42%), Query Frame = 0

Query: 4   SISTSAYPLLTSERSNRLVANDSSLSRLSSTSSLQLPVQLRCVRLGN-NGVSAPSRSRIL 63
           S+S+S  P L S+ S+ + A     SR  S    Q PV  R VR G+    S  S +R  
Sbjct: 5   SLSSSTVPSLNSKESSGVSA---FASRSISAVKFQFPV--RRVRTGDLKFPSLSSTTRCT 64

Query: 64  P-VVKAKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILEQVSATLSDTVQVVKI 123
           P  ++AK QTF SF+DLL NSDKPVLVD+YATWCGPCQFMVPIL +VS TL D +QVVKI
Sbjct: 65  PRRIEAKKQTFDSFEDLLVNSDKPVLVDYYATWCGPCQFMVPILNEVSETLKDKIQVVKI 124

Query: 124 DTEKYPVIADKYRIEALPTFILFKDGKPLDRFEGALTAVELVKRIEDSLKVK 174
           DTEKYP IA+KY+IEALPTFILFKDG+P DRFEGALTA +L++RIEDSLKVK
Sbjct: 125 DTEKYPSIANKYKIEALPTFILFKDGEPCDRFEGALTAKQLIQRIEDSLKVK 171

BLAST of Spg004499 vs. TAIR 10
Match: AT1G43560.1 (thioredoxin Y2 )

HSP 1 Score: 184.9 bits (468), Expect = 5.6e-47
Identity = 108/176 (61.36%), Postives = 128/176 (72.73%), Query Frame = 0

Query: 1   MAASISTS-AYPLLTSERSNRLVANDSSLSRLSSTSSLQLPVQLRCVRLGN-NGVSAPSR 60
           MA S++T+   P  T E SN       S S   + SS++LP Q+R  R G+    S+ +R
Sbjct: 1   MAISLATAYISPCFTPESSN-------SASPSRTLSSVRLPSQIR--RFGSVQSPSSSTR 60

Query: 61  SRILPVVKAKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILEQVSATLSDTVQV 120
              L V  AK QTF+SFDDLL NSDKPVLVDFYATWCGPCQ MVPIL +VS TL D + V
Sbjct: 61  FAPLTVRAAKKQTFNSFDDLLQNSDKPVLVDFYATWCGPCQLMVPILNEVSETLKDIIAV 120

Query: 121 VKIDTEKYPVIADKYRIEALPTFILFKDGKPLDRFEGALTAVELVKRIEDSLKVKQ 175
           VKIDTEKYP +A+KY+IEALPTFILFKDGK  DRFEGAL A +LV+RIE+SL+VKQ
Sbjct: 121 VKIDTEKYPSLANKYQIEALPTFILFKDGKLWDRFEGALPANQLVERIENSLQVKQ 167

BLAST of Spg004499 vs. TAIR 10
Match: AT1G50320.1 (thioredoxin X )

HSP 1 Score: 86.7 bits (213), Expect = 2.1e-17
Identity = 43/100 (43.00%), Postives = 62/100 (62.00%), Query Frame = 0

Query: 73  SSFDDLLANSDKPVLVDFYATWCGPCQFMVPILEQVSATLSDTVQVVKIDTEKYPVIADK 132
           S F   +  S +PVLV+F ATWCGPC+ + P +E +S    D + +VKID +  P +  +
Sbjct: 77  SEFSSTVLESAQPVLVEFVATWCGPCKLIYPAMEALSQEYGDKLTIVKIDHDANPKLIAE 136

Query: 133 YRIEALPTFILFKDGK--PLDRFEGALTAVELVKRIEDSL 171
           +++  LP FILFKDGK  P  R EGA+T  +L + I+  L
Sbjct: 137 FKVYGLPHFILFKDGKEVPGSRREGAITKAKLKEYIDGLL 176

BLAST of Spg004499 vs. TAIR 10
Match: AT3G15360.1 (thioredoxin M-type 4 )

HSP 1 Score: 81.3 bits (199), Expect = 8.7e-16
Identity = 55/173 (31.79%), Postives = 94/173 (54.34%), Query Frame = 0

Query: 1   MAASI-STSAYPLLTSERSN-----RLVANDSSLSRLSSTSSLQLPVQLRCVRLGNNGVS 60
           +AAS+ S+SA P ++  R +           SS S ++ ++SL    + R  R G   ++
Sbjct: 18  IAASVSSSSAAPSVSRRRISPARFLEFRGLKSSRSLVTQSASLGANRRTRIARGGR--IA 77

Query: 61  APSRSRILPVVKAKNQTFSSFDDLLANSDKPVLVDFYATWCGPCQFMVPILEQVSATLSD 120
             ++      V+  N + S +   +  SD PVLV+F+A WCGPC+ + PI++Q++   + 
Sbjct: 78  CEAQDTTAAAVEVPNLSDSEWQTKVLESDVPVLVEFWAPWCGPCRMIHPIVDQLAKDFAG 137

Query: 121 TVQVVKIDTEKYPVIADKYRIEALPTFILFKDGKPLDRFEGALTAVELVKRIE 168
             +  KI+T++ P  A++Y I ++PT I+FK G+  D   GA+    L K IE
Sbjct: 138 KFKFYKINTDESPNTANRYGIRSVPTVIIFKGGEKKDSIIGAVPRETLEKTIE 188

BLAST of Spg004499 vs. TAIR 10
Match: AT4G03520.1 (Thioredoxin superfamily protein )

HSP 1 Score: 72.4 bits (176), Expect = 4.0e-13
Identity = 54/187 (28.88%), Postives = 94/187 (50.27%), Query Frame = 0

Query: 1   MAASISTSAYPL-LTSERSNRLVANDSSLSRLSS---------TSSLQLPVQLRCVRLGN 60
           MAA   TS  P+ L SE   R+V++  S S LSS         +S L++ + L    L +
Sbjct: 1   MAAFTCTSRPPISLRSE--TRIVSSSPSASSLSSRRMFAVLPESSGLRIRLSLSPASLTS 60

Query: 61  NGVSAPSRSRILPVVKAKNQTF-------SSFDDLLANSDKPVLVDFYATWCGPCQFMVP 120
                 SR R   V +A+  T        S++D L+  +  PV+VDF+A WCGPC+ + P
Sbjct: 61  IHQPRVSRLRRAVVCEAQETTTDIQVVNDSTWDSLVLKATGPVVVDFWAPWCGPCKMIDP 120

Query: 121 ILEQVSATLSDTVQVVKIDTEKYPVIADKYRIEALPTFILFKDGKPLDRFEGALTAVELV 171
           ++  ++   +  ++  K++T++ P    +Y + ++PT ++F  G+  D   GA+    L 
Sbjct: 121 LVNDLAQHYTGKIKFYKLNTDESPNTPGQYGVRSIPTIMIFVGGEKKDTIIGAVPKTTLT 180

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022974037.14.4e-7889.66thioredoxin Y1, chloroplastic-like [Cucurbita maxima] >XP_022974038.1 thioredoxi... [more]
XP_022972902.13.7e-7788.51uncharacterized protein LOC111471411 [Cucurbita maxima][more]
XP_023520827.18.3e-7789.08uncharacterized protein LOC111784322 isoform X1 [Cucurbita pepo subsp. pepo] >XP... [more]
XP_022932802.11.8e-7687.36thioredoxin Y2, chloroplastic [Cucurbita moschata][more]
XP_022932509.17.0e-7688.51uncharacterized protein LOC111438908 [Cucurbita moschata] >KAG6607564.1 Thioredo... [more]
Match NameE-valueIdentityDescription
Q6NPF93.8e-4863.37Thioredoxin Y1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g76760 PE=1 ... [more]
Q8L7S97.9e-4661.36Thioredoxin Y2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g43560 PE=2 ... [more]
Q5JMR98.4e-4055.62Thioredoxin Y, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g09... [more]
P522323.9e-2956.44Thioredoxin-like protein slr0233 OS=Synechocystis sp. (strain PCC 6803 / Kazusa)... [more]
O309743.1e-1843.14Thioredoxin OS=Mycolicibacterium smegmatis OX=1772 GN=trxA PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1I9642.1e-7889.66thioredoxin Y1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111472651 P... [more]
A0A6J1I6281.8e-7788.51uncharacterized protein LOC111471411 OS=Cucurbita maxima OX=3661 GN=LOC111471411... [more]
A0A6J1EXS88.9e-7787.36thioredoxin Y2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111439262 PE=4... [more]
A0A6J1EX663.4e-7688.51uncharacterized protein LOC111438908 OS=Cucurbita moschata OX=3662 GN=LOC1114389... [more]
A0A6J1DH529.9e-7687.43thioredoxin Y2, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC11102001... [more]
Match NameE-valueIdentityDescription
AT1G76760.12.7e-4963.37thioredoxin Y1 [more]
AT1G43560.15.6e-4761.36thioredoxin Y2 [more]
AT1G50320.12.1e-1743.00thioredoxin X [more]
AT3G15360.18.7e-1631.79thioredoxin M-type 4 [more]
AT4G03520.14.0e-1328.88Thioredoxin superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00421THIOREDOXINcoord: 134..145
score: 35.93
coord: 94..103
score: 55.39
coord: 86..94
score: 64.32
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 68..174
e-value: 1.6E-32
score: 113.7
NoneNo IPR availablePANTHERPTHR45663:SF15THIOREDOXIN Y1, CHLOROPLASTICcoord: 3..173
NoneNo IPR availablePANTHERPTHR45663GEO12009P1coord: 3..173
NoneNo IPR availableCDDcd02947TRX_familycoord: 73..167
e-value: 5.91467E-36
score: 118.43
IPR013766Thioredoxin domainPFAMPF00085Thioredoxincoord: 70..167
e-value: 4.3E-29
score: 100.5
IPR013766Thioredoxin domainPROSITEPS51352THIOREDOXIN_2coord: 49..171
score: 16.519257
IPR005746ThioredoxinTIGRFAMTIGR01068TIGR01068coord: 72..168
e-value: 5.7E-32
score: 107.9
IPR017937Thioredoxin, conserved sitePROSITEPS00194THIOREDOXIN_1coord: 87..105
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 71..171

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg004499.1Spg004499.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006662 glycerol ether metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016671 oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor
molecular_function GO:0015035 protein-disulfide reductase activity