Spg004094 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg004094
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptiontobamovirus multiplication protein 1-like
Locationscaffold4: 3015493 .. 3019401 (-)
RNA-Seq ExpressionSpg004094
SyntenySpg004094
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAGGGCTTTACCGATCACCTCTGCGGTTGGTGGATTCGAATTGTGGAACGAGATTGATGAATCGAAGCAATGGCAGAAGGGGATTTACTCCGCCCTGTCCGCCTCTTATGGCCTCATCTCGCTCATTGCCTTAGTACTCCACTTTTCTCTAATCTTTACTGTCTTTTGTTCTGTATCTTGTGAAATTCATGCGTTATTCAAACCTTTTCTTGCACAATTCTTTTTCTTTTTTCTTTTTCTTTTTTGGGTTGAGTTCATATATCTTTATTGGAACTAACATGGCTTGGGATTAAATCCATCTTCCACATTGAACTCTCATTTGTCATCAAATTAACGGAATTAAGACATCTCTCTCATTTTTTTTAAAAGGAGTTTGAAGTACAATAGACTCTCTGTGGTTGTGCTGTGTGTGTGATCTGATGAAAATGGTTTGAACAACTGATTTTTAGTTTACTTTTAAGATATTATTATCTGGGTTCCACACTTATATGAGTTGGGATTTAATCCTTGGGTAATTAAGAGTGATCAATCAATGTCAGAAATTATATTGCAATCTTTAGAATGAGATTCCAAAGTGTTGTAGTGGAGGATGTTTCTGGAGCATTTATGAAGCTCTATATTGCTAGCTATCGCACGTTGGTTAGGAAACAAACCCGGGACTAGTGTATCGGTAAGAGTCTCACAAACTCGATTAATTATGTACCGAATCAATTGTCAAATTTGATGAATGCTAGTCACTGGCACAACCCTTGGACACCTTGTAAAGGTTGCATTTGTTGTACTTTGTATCTAAGCATTGTTGCATTTTCGGGCACGTGGAAGAAAAGTTGTTGCTTTGCCACTCTCCATTTTGTTATTAGGGTTTTACTCTTTGGTGCTCTGCTTTTTCTGCCACCTTGCTACCTTATGGGGGATTTGATCCGATAAAAAGGAGAATTTTCAATAGTGCTGAGAGGTCTTGGGAAGATATTTAGTCTCGTGTTAAATTTTATACTTCCCTTTGAGCATCGGTGTCAAAGGAATTTCATAACCATTCTTTAGACATGGTTCTCTTGGATTGGAGCTCTTTTTTGTATAGCTAGTTAGTTCTTTGGTAGCTGCTTTTGTTAAGGTATGTCCTTTTGTATTCTTTAATTTTGGTCAATGATAGTTGGTTTCTCGTAAAAAAAAAAAGTTACTTAAATTAAATTAATTCGCTGTCTTGGGGAAAAAAACACACAACTTTTGGTTTTTATAATTAACGCCTAATACTTAGAGTAATTGCTAAAGTTTCCAGGTTCAACTTATTCGGATTCAAATAAGAGTACCAGAATTCGAGTGGACAACACAAAAGGGGTTCCATTTGATGAATTTCATTGTAAATGGATGTAAGATTTTATATTTGTTAATTTTCATTTAGTTTAAGATTTTACTTGATGAGTTAATGCTACGTTATTTTCATTTTGATAGTGAGGGCTATTCTCTTTGGGCTTTATAAGAGCGTCTTCTTCATTAGACCGAAGGTAGGTTTCTTATATTCATTGTCACTATGCCAACTATGATTGTCTTAAATTCTTAATGCCCTGTTCTAGCCATGGTTATCACCAGATGGTCGAGTATATCTATGGGCCTCATTCTGATATTGTTTTTGGATTATTCAAGAGTTGTATTATGACAATCTAAACTAAAACTTCTGATTGATCTTATTATAAGCACCTTCTCTTTGTTATTAGCTTACATGTTACTTCAAAACATTTCTTGAATATTCAATTTATTCATTCAACAAAGCTTTCATTCAACATGCTATTATTACTTGAAGAGAAGAAAAGGGAGAAAAGAAAGAGAAACTAATAACAGTTATTAATGCACAACTAGATTATTGTTTATGTATTATTTTGTTTTCATATTTGTATTCGTATTTGTGGTAATACCATATTGAAATTTATTTATTTCCGAACTATGAAACATACAGAAGTCTTTCTGAAGGCAAAACTAATAAATAAAAACAAGCAAACAAAAGCCTTGTTTCCTGCTTTCTGTTGGATGCTTGGTAAAAAAAATCCAGGGATTTCTTCTTCCTCTGACGCTGGGAACCATAGCATTTTTAGAGAAAGATTTGTGGCATTATGTAATTTTGTTGCCCATCATAATCCACAGTCAGCAGAGCATCATTTTCCCATATCAAAAAAGCAAGTCAATATACAGACTTAAAATTGATAATCTAGATATTCTGAGAGACCCACGTTCCTTTTCCCATGATGTAACTGTGATTATTTTCTCTGAACTGTATGGCTTGAAACTTTTATGCTGGTAAAGGTTCTTGAAATGGTGGTTATGGAGATTCCTGGACTTCTATTCTTTTCAACGTATACACTACTTGTTCTATTTTGGGCGGAGATATACCATCAGGTAATTTATTATTATGATTATTATTTAATCTGAACGAAGTTATGTTACATATTATGTCAATTCTGTGCAAGATCCATCATTAGTAATCCATATAGGTGATCATTTTTCTTTCAGGCACGAAGTCTTCCTATTGATAAACTTAAGCCTACTTACTGCATCATCAATGGTGTTATGTACGTTATACAGGTAAGATAGAAGATGTCACAACCAAAAGTTGTCAGTACCATGTGAAAACAGAACGAACTGAATTTGGTTGATTCTCTTACATTCAACTTTGTTTCAATTATTTGTCATCATGTTTCAGATTTGCATTTGGATATTTGTGATGTTAAACCAATCTCCTGGTGCAGTTATAGCAGCTAAGCTCTTCTTTTCAGGTTGCAGCTTGAAACCTGCATTCTTTTTCTCTTATGTCAAAAGCAAATACTATTTTTTATTTTCATTAGAAATGTGATGTATAATTGATGTCCATTTTCAACTGGAACCTTTTGGCTTCCACATCCTAGTTTATGGAGGCATATGTAAATTATAATTTCTTTCTTTATTATTTCCAGTGGTTTCATTTTCTGCTGCACTGGGTTTCCTCATATATGGTGGGAGGTAATATATGTTTATGAATTCAGGAGCCGTGGTTAACTATGTTCCACTCTAACAATTTGTCTCTTAACAGGTTATTTGTCATGCTGAGGCAGTTCCCTATTGAATCTAGAGGACGCCAAAAAAAGTTGTACGAGGTTGGTTTCTTAATATGTTCTTTTATAGACTACATTTGAAAAAACATAAAAAGGTACTGTAGGATAAGACATGTTCGAAGCAATAGAGTTTAAGAAAGCCACATATGTCATCTGGTCTTTGAAGTAAACATCAATTTTCAATTTTTTCCCTGATCTTCATCTTCTAAAAGTACTATTTTATCCCCGTGTATTTTACAAATTTACCCCTGCTCAATAATGCCACTGAAATTGCATATTATATTAGAAGGGTGTTGTGACATTTTATAAAAAACAAATGGGCTCTATGCCGGCCATAGCATCACAAAGTATTTCATAGGCTCATCTAAGTCCACCCCAGTAAGAAATGATCATAAGATATTTCAAATGCCACGCCATTGTTCCATTATTCAATTTCATTAAGGATTTGATTGAAAAATTGAAATGATATTGAGCTTTAACATCAAGGATATAATTGAAGAATGGTGTATATCTCAGGGATCAAGGATAAATTTAGCCTTTTAAAAGATTAAGATTCTCTGAAGCTCTTGTTGATTTCTTACTTTTGGGTATCATTGCAGGTTGGTTGTGTGACAACAATTTGTTTTAGCTGTTTCTTTATACGATGTTTTGTGGTGAGTTTCCTTTTGGTTACAGTAATCTTGATCTGACATCTTCAAGCAATCAATACATAAATATATTTGTTCATCTTGTAGCTTGCCCTCTCCGCATTTGACAAGGATGCAGATCTTGACGTCCTAGATCATCCCATTCTCAACCTGATATACTACATGGCGAGTTTTTAA

mRNA sequence

ATGGCGAGGGCTTTACCGATCACCTCTGCGGTTGGTGGATTCGAATTGTGGAACGAGATTGATGAATCGAAGCAATGGCAGAAGGGGATTTACTCCGCCCTGTCCGCCTCTTATGGCCTCATCTCGCTCATTGCCTTAGTACTCCACTTTTCTCTAATCTTTACTGTCTTTTGTTCTGTATCTTGTGAAATTCATGCAATGAGATTCCAAAGTGTTGTAGTGGAGGATGTTTCTGGAGCATTTATGAAGCTCTATATTGCTAGCTATCGCACGTTGCTAGTTAGTTCTTTGGTAGCTGCTTTTGTTAAGGTTCAACTTATTCGGATTCAAATAAGAGTACCAGAATTCGAGTGGACAACACAAAAGGGGTTCCATTTGATGAATTTCATTGTAAATGGATTGAGGGCTATTCTCTTTGGGCTTTATAAGAGCGTCTTCTTCATTAGACCGAAGGTTCTTGAAATGGTGGTTATGGAGATTCCTGGACTTCTATTCTTTTCAACGTATACACTACTTGTTCTATTTTGGGCGGAGATATACCATCAGGCACGAAGTCTTCCTATTGATAAACTTAAGCCTACTTACTGCATCATCAATGGTGTTATGTACGTTATACAGATTTGCATTTGGATATTTGTGATGTTAAACCAATCTCCTGGTGCAGTTATAGCAGCTAAGCTCTTCTTTTCAGTGGTTTCATTTTCTGCTGCACTGGGTTTCCTCATATATGGTGGGAGGTTATTTGTCATGCTGAGGCAGTTCCCTATTGAATCTAGAGGACGCCAAAAAAAGTTGTACGAGGTTGGTTGTGTGACAACAATTTGTTTTAGCTGTTTCTTTATACGATGTTTTGTGCTTGCCCTCTCCGCATTTGACAAGGATGCAGATCTTGACGTCCTAGATCATCCCATTCTCAACCTGATATACTACATGGCGAGTTTTTAA

Coding sequence (CDS)

ATGGCGAGGGCTTTACCGATCACCTCTGCGGTTGGTGGATTCGAATTGTGGAACGAGATTGATGAATCGAAGCAATGGCAGAAGGGGATTTACTCCGCCCTGTCCGCCTCTTATGGCCTCATCTCGCTCATTGCCTTAGTACTCCACTTTTCTCTAATCTTTACTGTCTTTTGTTCTGTATCTTGTGAAATTCATGCAATGAGATTCCAAAGTGTTGTAGTGGAGGATGTTTCTGGAGCATTTATGAAGCTCTATATTGCTAGCTATCGCACGTTGCTAGTTAGTTCTTTGGTAGCTGCTTTTGTTAAGGTTCAACTTATTCGGATTCAAATAAGAGTACCAGAATTCGAGTGGACAACACAAAAGGGGTTCCATTTGATGAATTTCATTGTAAATGGATTGAGGGCTATTCTCTTTGGGCTTTATAAGAGCGTCTTCTTCATTAGACCGAAGGTTCTTGAAATGGTGGTTATGGAGATTCCTGGACTTCTATTCTTTTCAACGTATACACTACTTGTTCTATTTTGGGCGGAGATATACCATCAGGCACGAAGTCTTCCTATTGATAAACTTAAGCCTACTTACTGCATCATCAATGGTGTTATGTACGTTATACAGATTTGCATTTGGATATTTGTGATGTTAAACCAATCTCCTGGTGCAGTTATAGCAGCTAAGCTCTTCTTTTCAGTGGTTTCATTTTCTGCTGCACTGGGTTTCCTCATATATGGTGGGAGGTTATTTGTCATGCTGAGGCAGTTCCCTATTGAATCTAGAGGACGCCAAAAAAAGTTGTACGAGGTTGGTTGTGTGACAACAATTTGTTTTAGCTGTTTCTTTATACGATGTTTTGTGCTTGCCCTCTCCGCATTTGACAAGGATGCAGATCTTGACGTCCTAGATCATCCCATTCTCAACCTGATATACTACATGGCGAGTTTTTAA

Protein sequence

MARALPITSAVGGFELWNEIDESKQWQKGIYSALSASYGLISLIALVLHFSLIFTVFCSVSCEIHAMRFQSVVVEDVSGAFMKLYIASYRTLLVSSLVAAFVKVQLIRIQIRVPEFEWTTQKGFHLMNFIVNGLRAILFGLYKSVFFIRPKVLEMVVMEIPGLLFFSTYTLLVLFWAEIYHQARSLPIDKLKPTYCIINGVMYVIQICIWIFVMLNQSPGAVIAAKLFFSVVSFSAALGFLIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICFSCFFIRCFVLALSAFDKDADLDVLDHPILNLIYYMASF
Homology
BLAST of Spg004094 vs. NCBI nr
Match: XP_038888866.1 (tobamovirus multiplication protein 1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 441.4 bits (1134), Expect = 6.4e-120
Identity = 238/312 (76.28%), Postives = 244/312 (78.21%), Query Frame = 0

Query: 1   MARAL-PITSAVGGFELWNEIDESKQWQKGIYSALSASYGLISLIALVLHFSLIFTVFCS 60
           MARAL  ITS VGG + WNEIDES+ WQ+GIY  LSASYGLISLIAL             
Sbjct: 1   MARALATITSTVGGLQWWNEIDESQDWQRGIYYTLSASYGLISLIAL------------- 60

Query: 61  VSCEIHAMRFQSVVVEDVSGAFMKLYIASYRTLLVSSLVAAFVKVQLIRIQIRVPEFEWT 120
                                                       VQLIRIQIRVPEFEWT
Sbjct: 61  --------------------------------------------VQLIRIQIRVPEFEWT 120

Query: 121 TQKGFHLMNFIVNGLRAILFGLYKSVFFIRPKVLEMVVMEIPGLLFFSTYTLLVLFWAEI 180
           TQKGFHLMNFIVNGLRAILFGLYKSVF IRPKV+EMVVMEIPGLLFFSTYTLLVLFWAEI
Sbjct: 121 TQKGFHLMNFIVNGLRAILFGLYKSVFLIRPKVVEMVVMEIPGLLFFSTYTLLVLFWAEI 180

Query: 181 YHQARSLPIDKLKPTYCIINGVMYVIQICIWIFVMLNQSPGAVIAAKLFFSVVSFSAALG 240
           YHQARSLPIDKLKPTYCIINGVMYVIQICIWI VML+ SPG+VIAAKLFFSVVSFSAALG
Sbjct: 181 YHQARSLPIDKLKPTYCIINGVMYVIQICIWIIVMLDHSPGSVIAAKLFFSVVSFSAALG 240

Query: 241 FLIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICFSCFFIRCFVLALSAFDKDADLDV 300
           FLIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICFSCFFIRCFVLALSAFDKDADLDV
Sbjct: 241 FLIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICFSCFFIRCFVLALSAFDKDADLDV 255

Query: 301 LDHPILNLIYYM 312
           LDHPILNLIYYM
Sbjct: 301 LDHPILNLIYYM 255

BLAST of Spg004094 vs. NCBI nr
Match: XP_022988878.1 (tobamovirus multiplication protein 1-like [Cucurbita maxima] >XP_022988879.1 tobamovirus multiplication protein 1-like [Cucurbita maxima])

HSP 1 Score: 440.3 bits (1131), Expect = 1.4e-119
Identity = 232/311 (74.60%), Postives = 243/311 (78.14%), Query Frame = 0

Query: 1   MARALPITSAVGGFELWNEIDESKQWQKGIYSALSASYGLISLIALVLHFSLIFTVFCSV 60
           M R L ITSAVGGFE WN+IDES+ WQKGIY ALSASYGLIS+IAL              
Sbjct: 1   MVRDLVITSAVGGFEWWNQIDESEDWQKGIYYALSASYGLISIIAL-------------- 60

Query: 61  SCEIHAMRFQSVVVEDVSGAFMKLYIASYRTLLVSSLVAAFVKVQLIRIQIRVPEFEWTT 120
                                                      VQLIRIQ+RVPEFEWTT
Sbjct: 61  -------------------------------------------VQLIRIQVRVPEFEWTT 120

Query: 121 QKGFHLMNFIVNGLRAILFGLYKSVFFIRPKVLEMVVMEIPGLLFFSTYTLLVLFWAEIY 180
           QKGFHLMNF+VNGLRAILFGLYKSVF IRPK LEMVVME+PGLLFFSTYTLLVLFWAEIY
Sbjct: 121 QKGFHLMNFVVNGLRAILFGLYKSVFLIRPKALEMVVMEVPGLLFFSTYTLLVLFWAEIY 180

Query: 181 HQARSLPIDKLKPTYCIINGVMYVIQICIWIFVMLNQSPGAVIAAKLFFSVVSFSAALGF 240
           +QARSLPIDKLKPTYCIINGV+YVIQICIWIFVMLN+SPGAVI AKLFFSVVS SAALGF
Sbjct: 181 YQARSLPIDKLKPTYCIINGVIYVIQICIWIFVMLNKSPGAVIGAKLFFSVVSLSAALGF 240

Query: 241 LIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICFSCFFIRCFVLALSAFDKDADLDVL 300
           LIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICF+CFFIRCFVLA SAFDKD+DLDVL
Sbjct: 241 LIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICFTCFFIRCFVLAFSAFDKDSDLDVL 254

Query: 301 DHPILNLIYYM 312
           DHPILNLIYYM
Sbjct: 301 DHPILNLIYYM 254

BLAST of Spg004094 vs. NCBI nr
Match: XP_004136876.1 (tobamovirus multiplication protein 1 isoform X1 [Cucumis sativus] >KGN43722.1 hypothetical protein Csa_017225 [Cucumis sativus])

HSP 1 Score: 439.1 bits (1128), Expect = 3.2e-119
Identity = 235/312 (75.32%), Postives = 242/312 (77.56%), Query Frame = 0

Query: 1   MARAL-PITSAVGGFELWNEIDESKQWQKGIYSALSASYGLISLIALVLHFSLIFTVFCS 60
           MARAL  ITS +GGF+LWNEID+S  WQKGIY ALSASY LISLIAL             
Sbjct: 1   MARALSTITSTLGGFQLWNEIDDSDHWQKGIYYALSASYALISLIAL------------- 60

Query: 61  VSCEIHAMRFQSVVVEDVSGAFMKLYIASYRTLLVSSLVAAFVKVQLIRIQIRVPEFEWT 120
                                                       VQLIRIQIRVPEFEWT
Sbjct: 61  --------------------------------------------VQLIRIQIRVPEFEWT 120

Query: 121 TQKGFHLMNFIVNGLRAILFGLYKSVFFIRPKVLEMVVMEIPGLLFFSTYTLLVLFWAEI 180
           TQKGFHLMNFIVNGLRAILFGLYKSVF I+PKVLEMV+MEIPGLLFFSTYTLLVLFWAEI
Sbjct: 121 TQKGFHLMNFIVNGLRAILFGLYKSVFLIKPKVLEMVIMEIPGLLFFSTYTLLVLFWAEI 180

Query: 181 YHQARSLPIDKLKPTYCIINGVMYVIQICIWIFVMLNQSPGAVIAAKLFFSVVSFSAALG 240
           YHQARSLPI KLKPTYCI+NGVMY+IQICIWI VML  SPGAVI AKLFFSVVSFSAALG
Sbjct: 181 YHQARSLPISKLKPTYCIVNGVMYIIQICIWIIVMLEHSPGAVIVAKLFFSVVSFSAALG 240

Query: 241 FLIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICFSCFFIRCFVLALSAFDKDADLDV 300
           FLIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICFSCFFIRCFVLALSAFDKDADLDV
Sbjct: 241 FLIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICFSCFFIRCFVLALSAFDKDADLDV 255

Query: 301 LDHPILNLIYYM 312
           LDHPILNLIYYM
Sbjct: 301 LDHPILNLIYYM 255

BLAST of Spg004094 vs. NCBI nr
Match: XP_023530045.1 (tobamovirus multiplication protein 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 439.1 bits (1128), Expect = 3.2e-119
Identity = 232/311 (74.60%), Postives = 242/311 (77.81%), Query Frame = 0

Query: 1   MARALPITSAVGGFELWNEIDESKQWQKGIYSALSASYGLISLIALVLHFSLIFTVFCSV 60
           M R L ITSAVGGFE WN+IDES+ WQKGIY ALSASYGLIS+IAL              
Sbjct: 1   MVRDLVITSAVGGFEWWNQIDESEDWQKGIYYALSASYGLISIIAL-------------- 60

Query: 61  SCEIHAMRFQSVVVEDVSGAFMKLYIASYRTLLVSSLVAAFVKVQLIRIQIRVPEFEWTT 120
                                                      VQLIRIQ+RVPEFEWTT
Sbjct: 61  -------------------------------------------VQLIRIQVRVPEFEWTT 120

Query: 121 QKGFHLMNFIVNGLRAILFGLYKSVFFIRPKVLEMVVMEIPGLLFFSTYTLLVLFWAEIY 180
           QKGFHLMNF+VNGLRAILFGLYKSVF IRPK LEMVVME+PGLLFFSTYTLLVLFWAEIY
Sbjct: 121 QKGFHLMNFVVNGLRAILFGLYKSVFLIRPKALEMVVMEVPGLLFFSTYTLLVLFWAEIY 180

Query: 181 HQARSLPIDKLKPTYCIINGVMYVIQICIWIFVMLNQSPGAVIAAKLFFSVVSFSAALGF 240
           +QARSLPIDKLKPTYCIINGV+Y+IQICIWIFVMLN+SPGAVI AKLFFSVVS SAALGF
Sbjct: 181 YQARSLPIDKLKPTYCIINGVIYLIQICIWIFVMLNKSPGAVIGAKLFFSVVSLSAALGF 240

Query: 241 LIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICFSCFFIRCFVLALSAFDKDADLDVL 300
           LIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICF CFFIRCFVLA SAFDKDADLDVL
Sbjct: 241 LIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICFICFFIRCFVLAFSAFDKDADLDVL 254

Query: 301 DHPILNLIYYM 312
           DHPILNLIYYM
Sbjct: 301 DHPILNLIYYM 254

BLAST of Spg004094 vs. NCBI nr
Match: XP_022927929.1 (tobamovirus multiplication protein 1-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 438.7 bits (1127), Expect = 4.2e-119
Identity = 231/311 (74.28%), Postives = 242/311 (77.81%), Query Frame = 0

Query: 1   MARALPITSAVGGFELWNEIDESKQWQKGIYSALSASYGLISLIALVLHFSLIFTVFCSV 60
           M R L ITSAVGGFE WN+IDES+ WQKGIY  LSASYGLIS+IAL              
Sbjct: 1   MVRDLVITSAVGGFEWWNQIDESEDWQKGIYYTLSASYGLISIIAL-------------- 60

Query: 61  SCEIHAMRFQSVVVEDVSGAFMKLYIASYRTLLVSSLVAAFVKVQLIRIQIRVPEFEWTT 120
                                                      VQLIRIQ+RVPEFEWTT
Sbjct: 61  -------------------------------------------VQLIRIQVRVPEFEWTT 120

Query: 121 QKGFHLMNFIVNGLRAILFGLYKSVFFIRPKVLEMVVMEIPGLLFFSTYTLLVLFWAEIY 180
           QKGFHLMNF+VNGLRAILFGLYKSVF IRPK LEMVVME+PGLLFFSTYTLLVLFWAEIY
Sbjct: 121 QKGFHLMNFVVNGLRAILFGLYKSVFLIRPKALEMVVMEVPGLLFFSTYTLLVLFWAEIY 180

Query: 181 HQARSLPIDKLKPTYCIINGVMYVIQICIWIFVMLNQSPGAVIAAKLFFSVVSFSAALGF 240
           +QARSLPID+LKPTYCIINGV+YVIQICIWIFVMLN+SPGAVI AKLFFSVVS SAALGF
Sbjct: 181 YQARSLPIDRLKPTYCIINGVIYVIQICIWIFVMLNKSPGAVIGAKLFFSVVSLSAALGF 240

Query: 241 LIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICFSCFFIRCFVLALSAFDKDADLDVL 300
           LIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICF+CFFIRCFVLA SAFDKDADLDVL
Sbjct: 241 LIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICFTCFFIRCFVLAFSAFDKDADLDVL 254

Query: 301 DHPILNLIYYM 312
           DHPILNLIYYM
Sbjct: 301 DHPILNLIYYM 254

BLAST of Spg004094 vs. ExPASy Swiss-Prot
Match: Q402F4 (Tobamovirus multiplication protein 1 OS=Nicotiana tabacum OX=4097 GN=TOM1 PE=1 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 1.1e-82
Identity = 157/295 (53.22%), Postives = 197/295 (66.78%), Query Frame = 0

Query: 17  WNEIDESKQWQKGIYSALSASYGLISLIALVLHFSLIFTVFCSVSCEIHAMRFQSVVVED 76
           W++I+ES QWQ GI+ +L ASY L+S +AL                              
Sbjct: 21  WDQINESVQWQDGIFYSLCASYALVSAVAL------------------------------ 80

Query: 77  VSGAFMKLYIASYRTLLVSSLVAAFVKVQLIRIQIRVPEFEWTTQKGFHLMNFIVNGLRA 136
                                      +QLIRI++RVPE+ WTTQK FHLMNF+VNG+RA
Sbjct: 81  ---------------------------IQLIRIELRVPEYGWTTQKVFHLMNFVVNGVRA 140

Query: 137 ILFGLYKSVFFIRPKVLEMVVMEIPGLLFFSTYTLLVLFWAEIYHQARSLPIDKLKPTYC 196
           I+FG +K VF   PKVL + ++++PGLLFFST+TLLVLFWAEIYHQARSLP DKL+ +Y 
Sbjct: 141 IVFGFHKQVFLFHPKVLSLAILDLPGLLFFSTFTLLVLFWAEIYHQARSLPTDKLRISYI 200

Query: 197 IINGVMYVIQICIWIFVMLNQSPGAVIAAKLFFSVVSFSAALGFLIYGGRLFVMLRQFPI 256
            ING +Y IQ CIW+++  N +       K+F +VVSF AALGFL+YGGRLF+MLR+FPI
Sbjct: 201 SINGAIYFIQACIWVYLWSNDNSTVEFIGKIFIAVVSFIAALGFLLYGGRLFLMLRRFPI 258

Query: 257 ESRGRQKKLYEVGCVTTICFSCFFIRCFVLALSAFDKDADLDVLDHPILNLIYYM 312
           ES+GR+KKL+EVG VT ICF+CF I CFV+ LSAFD DA LDVLDHP+LNLIYY+
Sbjct: 261 ESKGRRKKLHEVGSVTAICFTCFLISCFVVVLSAFDPDASLDVLDHPVLNLIYYL 258

BLAST of Spg004094 vs. ExPASy Swiss-Prot
Match: Q9FEG2 (Tobamovirus multiplication protein 1 OS=Arabidopsis thaliana OX=3702 GN=TOM1 PE=1 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 1.1e-79
Identity = 156/305 (51.15%), Postives = 200/305 (65.57%), Query Frame = 0

Query: 7   ITSAVGGFELWNEIDESKQWQKGIYSALSASYGLISLIALVLHFSLIFTVFCSVSCEIHA 66
           +T+A+  +  W++++ES QWQ GI+ AL  +Y L+S +AL                    
Sbjct: 16  LTTAITSW--WDDVNESTQWQDGIFFALCGAYALVSAVAL-------------------- 75

Query: 67  MRFQSVVVEDVSGAFMKLYIASYRTLLVSSLVAAFVKVQLIRIQIRVPEFEWTTQKGFHL 126
                                                VQLIRIQ+RVPE+ WTTQK FHL
Sbjct: 76  -------------------------------------VQLIRIQMRVPEYGWTTQKVFHL 135

Query: 127 MNFIVNGLRAILFGLYKSVFFIRPKVLEMVVMEIPGLLFFSTYTLLVLFWAEIYHQARSL 186
           MNF+VNG+RA+LFG +  VF + PK L  V++++PGLLFFS YTLLVLFWAEIYHQARSL
Sbjct: 136 MNFVVNGVRAVLFGFHMQVFLVHPKALCWVLLDLPGLLFFSAYTLLVLFWAEIYHQARSL 195

Query: 187 PIDKLKPTYCIINGVMYVIQICIWIFVMLNQSPGAVIAAKLFFSVVSFSAALGFLIYGGR 246
           P DKL+ TY  +N  +Y+ QI IW ++ ++ +    +  K+F +VVSF AALGFL+YGGR
Sbjct: 196 PTDKLRITYISVNVAVYLAQIGIWAYIWVHDNSTVELVGKIFIAVVSFIAALGFLLYGGR 255

Query: 247 LFVMLRQFPIESRGRQKKLYEVGCVTTICFSCFFIRCFVLALSAFDKDADLDVLDHPILN 306
           LF MLR+FPIES+GR+KKL+EVG VT ICF+CF IRC V+A+SAFDKD  LDVLDHP+LN
Sbjct: 256 LFFMLRRFPIESKGRRKKLHEVGSVTAICFTCFLIRCVVVAVSAFDKDLTLDVLDHPVLN 261

Query: 307 LIYYM 312
           LIYYM
Sbjct: 316 LIYYM 261

BLAST of Spg004094 vs. ExPASy Swiss-Prot
Match: Q9ZUM2 (Tobamovirus multiplication protein 3 OS=Arabidopsis thaliana OX=3702 GN=TOM3 PE=1 SV=2)

HSP 1 Score: 262.7 bits (670), Expect = 5.3e-69
Identity = 132/295 (44.75%), Postives = 191/295 (64.75%), Query Frame = 0

Query: 17  WNEIDESKQWQKGIYSALSASYGLISLIALVLHFSLIFTVFCSVSCEIHAMRFQSVVVED 76
           W++++ES  WQ  I+  L+  YG++SL+A+                              
Sbjct: 36  WSDVNESPIWQDRIFHVLAVLYGIVSLVAV------------------------------ 95

Query: 77  VSGAFMKLYIASYRTLLVSSLVAAFVKVQLIRIQIRVPEFEWTTQKGFHLMNFIVNGLRA 136
                                      +QL+RIQ+RVPE+ WTTQK FH +NF+VNG+RA
Sbjct: 96  ---------------------------IQLVRIQLRVPEYGWTTQKVFHFLNFVVNGVRA 155

Query: 137 ILFGLYKSVFFIRPKVLEMVVMEIPGLLFFSTYTLLVLFWAEIYHQARSLPIDKLKPTYC 196
           ++F   ++V F++P++L+ ++++IP L FF+TY LLVLFWAEIY+QAR++  D L+P++ 
Sbjct: 156 VVFVFRRNVQFMQPEILQHILLDIPSLAFFTTYALLVLFWAEIYYQARAVSTDGLRPSFF 215

Query: 197 IINGVMYVIQICIWIFVMLNQSPGAVIAAKLFFSVVSFSAALGFLIYGGRLFVMLRQFPI 256
            IN V+YV+QI +W+ +        VI +K+FF+ VS  AALGFL+YGGRLF+ML++FP+
Sbjct: 216 TINAVVYVVQIALWLVLWWKPVRVMVILSKMFFAGVSLFAALGFLLYGGRLFLMLQRFPV 273

Query: 257 ESRGRQKKLYEVGCVTTICFSCFFIRCFVLALSAFDKDADLDVLDHPILNLIYYM 312
           ES+GR+KKL EVG VTTICF+CF IRC ++  +AFD+ A+LDVLDHPILN IYY+
Sbjct: 276 ESKGRRKKLQEVGYVTTICFTCFLIRCIMMCFAAFDEGANLDVLDHPILNFIYYL 273

BLAST of Spg004094 vs. ExPASy Swiss-Prot
Match: Q402F3 (Tobamovirus multiplication protein 3 OS=Nicotiana tabacum OX=4097 GN=TOM3 PE=1 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 1.6e-68
Identity = 125/219 (57.08%), Postives = 174/219 (79.45%), Query Frame = 0

Query: 93  LVSSLVAAFVKVQLIRIQIRVPEFEWTTQKGFHLMNFIVNGLRAILFGLYKSVFFIRPKV 152
           ++  +V+A   VQLIRIQ+RVPE+ WTTQK FH +NF+VNG+R+++F   + V  + P++
Sbjct: 46  ILYGIVSAVALVQLIRIQMRVPEYGWTTQKVFHFLNFLVNGVRSLVFAFRRDVQKLHPEI 105

Query: 153 LEMVVMEIPGLLFFSTYTLLVLFWAEIYHQARSLPIDKLKPTYCIINGVMYVIQICIWIF 212
           ++ +++++P L FF+TY LLVLFWAEIY+QAR++  D L+P++  INGV+YVIQI +W+ 
Sbjct: 106 VQHILLDMPSLAFFTTYALLVLFWAEIYYQARAVSTDGLRPSFFTINGVVYVIQIILWLI 165

Query: 213 VMLNQSPGAVIAAKLFFSVVSFSAALGFLIYGGRLFVMLRQFPIESRGRQKKLYEVGCVT 272
           +        VI +K+FF+ VS  AALGFL+YGGRLF+ML++FP+ES+GR+KKL EVG VT
Sbjct: 166 IWWKPVRVLVILSKMFFAGVSLFAALGFLLYGGRLFLMLQRFPVESKGRRKKLQEVGYVT 225

Query: 273 TICFSCFFIRCFVLALSAFDKDADLDVLDHPILNLIYYM 312
           TICFSCF IRC ++  +AF+K ADLDVLDHPILNLIYY+
Sbjct: 226 TICFSCFLIRCVMMCFNAFNKAADLDVLDHPILNLIYYL 264

BLAST of Spg004094 vs. ExPASy Swiss-Prot
Match: Q948R8 (Protein TOM THREE HOMOLOG 1 OS=Arabidopsis thaliana OX=3702 GN=THH1 PE=2 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.1e-66
Identity = 136/310 (43.87%), Postives = 191/310 (61.61%), Query Frame = 0

Query: 8   TSAVGGFEL------WNEIDESKQWQKGIYSALSASYGLISLIALVLHFSLIFTVFCSVS 67
           +SA+  F L      W +++ES  WQ  I+  L+  YG++S+IA+               
Sbjct: 11  SSAIVAFNLKEGKNWWWDVNESPVWQDRIFHVLAVLYGIVSVIAV--------------- 70

Query: 68  CEIHAMRFQSVVVEDVSGAFMKLYIASYRTLLVSSLVAAFVKVQLIRIQIRVPEFEWTTQ 127
                                                     +QL+RIQ+RVPE+ WTTQ
Sbjct: 71  ------------------------------------------IQLVRIQLRVPEYGWTTQ 130

Query: 128 KGFHLMNFIVNGLRAILFGLYKSVFFIRPKVLEMVVMEIPGLLFFSTYTLLVLFWAEIYH 187
           K FH +NF+VNG+RA++F   +    ++P++L+ ++++IP L FF+TY LLVLFWAEIY+
Sbjct: 131 KVFHFLNFMVNGVRALVFLFRRDAQNMQPEILQHILLDIPSLAFFTTYALLVLFWAEIYY 190

Query: 188 QARSLPIDKLKPTYCIINGVMYVIQICIWIFVMLNQSPGAVIAAKLFFSVVSFSAALGFL 247
           QAR++  D L+P++  IN V+YVIQI +W+ +        VI +K+FF+ VS  AALGFL
Sbjct: 191 QARAVSTDGLRPSFFTINAVVYVIQIALWLVLWWKPVHLMVIISKMFFAGVSLFAALGFL 250

Query: 248 IYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICFSCFFIRCFVLALSAFDKDADLDVLD 307
           +YGGRLF+ML++FP+ES+GR+KKL EVG VTTICF+CF IRC ++   AFD  ADLDVLD
Sbjct: 251 LYGGRLFLMLQRFPVESKGRRKKLQEVGYVTTICFTCFLIRCIMMCFDAFDDAADLDVLD 263

Query: 308 HPILNLIYYM 312
           HPILN IYY+
Sbjct: 311 HPILNFIYYL 263

BLAST of Spg004094 vs. ExPASy TrEMBL
Match: A0A6J1JNL1 (tobamovirus multiplication protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111486099 PE=3 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 6.9e-120
Identity = 232/311 (74.60%), Postives = 243/311 (78.14%), Query Frame = 0

Query: 1   MARALPITSAVGGFELWNEIDESKQWQKGIYSALSASYGLISLIALVLHFSLIFTVFCSV 60
           M R L ITSAVGGFE WN+IDES+ WQKGIY ALSASYGLIS+IAL              
Sbjct: 1   MVRDLVITSAVGGFEWWNQIDESEDWQKGIYYALSASYGLISIIAL-------------- 60

Query: 61  SCEIHAMRFQSVVVEDVSGAFMKLYIASYRTLLVSSLVAAFVKVQLIRIQIRVPEFEWTT 120
                                                      VQLIRIQ+RVPEFEWTT
Sbjct: 61  -------------------------------------------VQLIRIQVRVPEFEWTT 120

Query: 121 QKGFHLMNFIVNGLRAILFGLYKSVFFIRPKVLEMVVMEIPGLLFFSTYTLLVLFWAEIY 180
           QKGFHLMNF+VNGLRAILFGLYKSVF IRPK LEMVVME+PGLLFFSTYTLLVLFWAEIY
Sbjct: 121 QKGFHLMNFVVNGLRAILFGLYKSVFLIRPKALEMVVMEVPGLLFFSTYTLLVLFWAEIY 180

Query: 181 HQARSLPIDKLKPTYCIINGVMYVIQICIWIFVMLNQSPGAVIAAKLFFSVVSFSAALGF 240
           +QARSLPIDKLKPTYCIINGV+YVIQICIWIFVMLN+SPGAVI AKLFFSVVS SAALGF
Sbjct: 181 YQARSLPIDKLKPTYCIINGVIYVIQICIWIFVMLNKSPGAVIGAKLFFSVVSLSAALGF 240

Query: 241 LIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICFSCFFIRCFVLALSAFDKDADLDVL 300
           LIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICF+CFFIRCFVLA SAFDKD+DLDVL
Sbjct: 241 LIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICFTCFFIRCFVLAFSAFDKDSDLDVL 254

Query: 301 DHPILNLIYYM 312
           DHPILNLIYYM
Sbjct: 301 DHPILNLIYYM 254

BLAST of Spg004094 vs. ExPASy TrEMBL
Match: A0A0A0K5Z8 (DUF1084 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G062880 PE=3 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 1.5e-119
Identity = 235/312 (75.32%), Postives = 242/312 (77.56%), Query Frame = 0

Query: 1   MARAL-PITSAVGGFELWNEIDESKQWQKGIYSALSASYGLISLIALVLHFSLIFTVFCS 60
           MARAL  ITS +GGF+LWNEID+S  WQKGIY ALSASY LISLIAL             
Sbjct: 1   MARALSTITSTLGGFQLWNEIDDSDHWQKGIYYALSASYALISLIAL------------- 60

Query: 61  VSCEIHAMRFQSVVVEDVSGAFMKLYIASYRTLLVSSLVAAFVKVQLIRIQIRVPEFEWT 120
                                                       VQLIRIQIRVPEFEWT
Sbjct: 61  --------------------------------------------VQLIRIQIRVPEFEWT 120

Query: 121 TQKGFHLMNFIVNGLRAILFGLYKSVFFIRPKVLEMVVMEIPGLLFFSTYTLLVLFWAEI 180
           TQKGFHLMNFIVNGLRAILFGLYKSVF I+PKVLEMV+MEIPGLLFFSTYTLLVLFWAEI
Sbjct: 121 TQKGFHLMNFIVNGLRAILFGLYKSVFLIKPKVLEMVIMEIPGLLFFSTYTLLVLFWAEI 180

Query: 181 YHQARSLPIDKLKPTYCIINGVMYVIQICIWIFVMLNQSPGAVIAAKLFFSVVSFSAALG 240
           YHQARSLPI KLKPTYCI+NGVMY+IQICIWI VML  SPGAVI AKLFFSVVSFSAALG
Sbjct: 181 YHQARSLPISKLKPTYCIVNGVMYIIQICIWIIVMLEHSPGAVIVAKLFFSVVSFSAALG 240

Query: 241 FLIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICFSCFFIRCFVLALSAFDKDADLDV 300
           FLIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICFSCFFIRCFVLALSAFDKDADLDV
Sbjct: 241 FLIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICFSCFFIRCFVLALSAFDKDADLDV 255

Query: 301 LDHPILNLIYYM 312
           LDHPILNLIYYM
Sbjct: 301 LDHPILNLIYYM 255

BLAST of Spg004094 vs. ExPASy TrEMBL
Match: A0A6J1EME9 (tobamovirus multiplication protein 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434684 PE=3 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 2.0e-119
Identity = 231/311 (74.28%), Postives = 242/311 (77.81%), Query Frame = 0

Query: 1   MARALPITSAVGGFELWNEIDESKQWQKGIYSALSASYGLISLIALVLHFSLIFTVFCSV 60
           M R L ITSAVGGFE WN+IDES+ WQKGIY  LSASYGLIS+IAL              
Sbjct: 1   MVRDLVITSAVGGFEWWNQIDESEDWQKGIYYTLSASYGLISIIAL-------------- 60

Query: 61  SCEIHAMRFQSVVVEDVSGAFMKLYIASYRTLLVSSLVAAFVKVQLIRIQIRVPEFEWTT 120
                                                      VQLIRIQ+RVPEFEWTT
Sbjct: 61  -------------------------------------------VQLIRIQVRVPEFEWTT 120

Query: 121 QKGFHLMNFIVNGLRAILFGLYKSVFFIRPKVLEMVVMEIPGLLFFSTYTLLVLFWAEIY 180
           QKGFHLMNF+VNGLRAILFGLYKSVF IRPK LEMVVME+PGLLFFSTYTLLVLFWAEIY
Sbjct: 121 QKGFHLMNFVVNGLRAILFGLYKSVFLIRPKALEMVVMEVPGLLFFSTYTLLVLFWAEIY 180

Query: 181 HQARSLPIDKLKPTYCIINGVMYVIQICIWIFVMLNQSPGAVIAAKLFFSVVSFSAALGF 240
           +QARSLPID+LKPTYCIINGV+YVIQICIWIFVMLN+SPGAVI AKLFFSVVS SAALGF
Sbjct: 181 YQARSLPIDRLKPTYCIINGVIYVIQICIWIFVMLNKSPGAVIGAKLFFSVVSLSAALGF 240

Query: 241 LIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICFSCFFIRCFVLALSAFDKDADLDVL 300
           LIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICF+CFFIRCFVLA SAFDKDADLDVL
Sbjct: 241 LIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICFTCFFIRCFVLAFSAFDKDADLDVL 254

Query: 301 DHPILNLIYYM 312
           DHPILNLIYYM
Sbjct: 301 DHPILNLIYYM 254

BLAST of Spg004094 vs. ExPASy TrEMBL
Match: A0A6J1EJD7 (tobamovirus multiplication protein 1-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111434684 PE=3 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 2.0e-119
Identity = 231/311 (74.28%), Postives = 242/311 (77.81%), Query Frame = 0

Query: 1   MARALPITSAVGGFELWNEIDESKQWQKGIYSALSASYGLISLIALVLHFSLIFTVFCSV 60
           M R L ITSAVGGFE WN+IDES+ WQKGIY  LSASYGLIS+IAL              
Sbjct: 1   MVRDLVITSAVGGFEWWNQIDESEDWQKGIYYTLSASYGLISIIAL-------------- 60

Query: 61  SCEIHAMRFQSVVVEDVSGAFMKLYIASYRTLLVSSLVAAFVKVQLIRIQIRVPEFEWTT 120
                                                      VQLIRIQ+RVPEFEWTT
Sbjct: 61  -------------------------------------------VQLIRIQVRVPEFEWTT 120

Query: 121 QKGFHLMNFIVNGLRAILFGLYKSVFFIRPKVLEMVVMEIPGLLFFSTYTLLVLFWAEIY 180
           QKGFHLMNF+VNGLRAILFGLYKSVF IRPK LEMVVME+PGLLFFSTYTLLVLFWAEIY
Sbjct: 121 QKGFHLMNFVVNGLRAILFGLYKSVFLIRPKALEMVVMEVPGLLFFSTYTLLVLFWAEIY 180

Query: 181 HQARSLPIDKLKPTYCIINGVMYVIQICIWIFVMLNQSPGAVIAAKLFFSVVSFSAALGF 240
           +QARSLPID+LKPTYCIINGV+YVIQICIWIFVMLN+SPGAVI AKLFFSVVS SAALGF
Sbjct: 181 YQARSLPIDRLKPTYCIINGVIYVIQICIWIFVMLNKSPGAVIGAKLFFSVVSLSAALGF 240

Query: 241 LIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICFSCFFIRCFVLALSAFDKDADLDVL 300
           LIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICF+CFFIRCFVLA SAFDKDADLDVL
Sbjct: 241 LIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICFTCFFIRCFVLAFSAFDKDADLDVL 254

Query: 301 DHPILNLIYYM 312
           DHPILNLIYYM
Sbjct: 301 DHPILNLIYYM 254

BLAST of Spg004094 vs. ExPASy TrEMBL
Match: A0A6J1HVA7 (tobamovirus multiplication protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467004 PE=3 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 6.1e-116
Identity = 228/311 (73.31%), Postives = 241/311 (77.49%), Query Frame = 0

Query: 1   MARALPITSAVGGFELWNEIDESKQWQKGIYSALSASYGLISLIALVLHFSLIFTVFCSV 60
           MARAL   SAV G E WN IDES+QWQKGIYSALS+SYGL+S+IAL              
Sbjct: 1   MARALATGSAVEGLEWWNGIDESEQWQKGIYSALSSSYGLLSIIAL-------------- 60

Query: 61  SCEIHAMRFQSVVVEDVSGAFMKLYIASYRTLLVSSLVAAFVKVQLIRIQIRVPEFEWTT 120
                                                      VQLIRIQIRVPEFEWTT
Sbjct: 61  -------------------------------------------VQLIRIQIRVPEFEWTT 120

Query: 121 QKGFHLMNFIVNGLRAILFGLYKSVFFIRPKVLEMVVMEIPGLLFFSTYTLLVLFWAEIY 180
           QKGFHLMNFIVNGLRAI+FGLYKSVF IRPKVLEMV+MEIPGLLFFSTYTLLVLFWAEIY
Sbjct: 121 QKGFHLMNFIVNGLRAIIFGLYKSVFLIRPKVLEMVIMEIPGLLFFSTYTLLVLFWAEIY 180

Query: 181 HQARSLPIDKLKPTYCIINGVMYVIQICIWIFVMLNQSPGAVIAAKLFFSVVSFSAALGF 240
           HQARSLPI KLKPTYCIINGVMY+IQICIWI VML+QSPGAVIAAKLFFSVV+FSAA+GF
Sbjct: 181 HQARSLPIRKLKPTYCIINGVMYIIQICIWIIVMLDQSPGAVIAAKLFFSVVAFSAAVGF 240

Query: 241 LIYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICFSCFFIRCFVLALSAFDKDADLDVL 300
           LIYGGRLFVMLRQFPIESRGRQKKLYEVG VT+ICFSCF IRC VLALSAFDKDADLDVL
Sbjct: 241 LIYGGRLFVMLRQFPIESRGRQKKLYEVGFVTSICFSCFSIRCLVLALSAFDKDADLDVL 254

Query: 301 DHPILNLIYYM 312
           DHP+LNLIYY+
Sbjct: 301 DHPVLNLIYYL 254

BLAST of Spg004094 vs. TAIR 10
Match: AT4G21790.1 (tobamovirus multiplication 1 )

HSP 1 Score: 298.1 bits (762), Expect = 8.2e-81
Identity = 156/305 (51.15%), Postives = 200/305 (65.57%), Query Frame = 0

Query: 7   ITSAVGGFELWNEIDESKQWQKGIYSALSASYGLISLIALVLHFSLIFTVFCSVSCEIHA 66
           +T+A+  +  W++++ES QWQ GI+ AL  +Y L+S +AL                    
Sbjct: 16  LTTAITSW--WDDVNESTQWQDGIFFALCGAYALVSAVAL-------------------- 75

Query: 67  MRFQSVVVEDVSGAFMKLYIASYRTLLVSSLVAAFVKVQLIRIQIRVPEFEWTTQKGFHL 126
                                                VQLIRIQ+RVPE+ WTTQK FHL
Sbjct: 76  -------------------------------------VQLIRIQMRVPEYGWTTQKVFHL 135

Query: 127 MNFIVNGLRAILFGLYKSVFFIRPKVLEMVVMEIPGLLFFSTYTLLVLFWAEIYHQARSL 186
           MNF+VNG+RA+LFG +  VF + PK L  V++++PGLLFFS YTLLVLFWAEIYHQARSL
Sbjct: 136 MNFVVNGVRAVLFGFHMQVFLVHPKALCWVLLDLPGLLFFSAYTLLVLFWAEIYHQARSL 195

Query: 187 PIDKLKPTYCIINGVMYVIQICIWIFVMLNQSPGAVIAAKLFFSVVSFSAALGFLIYGGR 246
           P DKL+ TY  +N  +Y+ QI IW ++ ++ +    +  K+F +VVSF AALGFL+YGGR
Sbjct: 196 PTDKLRITYISVNVAVYLAQIGIWAYIWVHDNSTVELVGKIFIAVVSFIAALGFLLYGGR 255

Query: 247 LFVMLRQFPIESRGRQKKLYEVGCVTTICFSCFFIRCFVLALSAFDKDADLDVLDHPILN 306
           LF MLR+FPIES+GR+KKL+EVG VT ICF+CF IRC V+A+SAFDKD  LDVLDHP+LN
Sbjct: 256 LFFMLRRFPIESKGRRKKLHEVGSVTAICFTCFLIRCVVVAVSAFDKDLTLDVLDHPVLN 261

Query: 307 LIYYM 312
           LIYYM
Sbjct: 316 LIYYM 261

BLAST of Spg004094 vs. TAIR 10
Match: AT2G02180.1 (tobamovirus multiplication protein 3 )

HSP 1 Score: 262.7 bits (670), Expect = 3.8e-70
Identity = 132/295 (44.75%), Postives = 191/295 (64.75%), Query Frame = 0

Query: 17  WNEIDESKQWQKGIYSALSASYGLISLIALVLHFSLIFTVFCSVSCEIHAMRFQSVVVED 76
           W++++ES  WQ  I+  L+  YG++SL+A+                              
Sbjct: 36  WSDVNESPIWQDRIFHVLAVLYGIVSLVAV------------------------------ 95

Query: 77  VSGAFMKLYIASYRTLLVSSLVAAFVKVQLIRIQIRVPEFEWTTQKGFHLMNFIVNGLRA 136
                                      +QL+RIQ+RVPE+ WTTQK FH +NF+VNG+RA
Sbjct: 96  ---------------------------IQLVRIQLRVPEYGWTTQKVFHFLNFVVNGVRA 155

Query: 137 ILFGLYKSVFFIRPKVLEMVVMEIPGLLFFSTYTLLVLFWAEIYHQARSLPIDKLKPTYC 196
           ++F   ++V F++P++L+ ++++IP L FF+TY LLVLFWAEIY+QAR++  D L+P++ 
Sbjct: 156 VVFVFRRNVQFMQPEILQHILLDIPSLAFFTTYALLVLFWAEIYYQARAVSTDGLRPSFF 215

Query: 197 IINGVMYVIQICIWIFVMLNQSPGAVIAAKLFFSVVSFSAALGFLIYGGRLFVMLRQFPI 256
            IN V+YV+QI +W+ +        VI +K+FF+ VS  AALGFL+YGGRLF+ML++FP+
Sbjct: 216 TINAVVYVVQIALWLVLWWKPVRVMVILSKMFFAGVSLFAALGFLLYGGRLFLMLQRFPV 273

Query: 257 ESRGRQKKLYEVGCVTTICFSCFFIRCFVLALSAFDKDADLDVLDHPILNLIYYM 312
           ES+GR+KKL EVG VTTICF+CF IRC ++  +AFD+ A+LDVLDHPILN IYY+
Sbjct: 276 ESKGRRKKLQEVGYVTTICFTCFLIRCIMMCFAAFDEGANLDVLDHPILNFIYYL 273

BLAST of Spg004094 vs. TAIR 10
Match: AT1G14530.1 (Protein of unknown function (DUF1084) )

HSP 1 Score: 255.0 bits (650), Expect = 7.9e-68
Identity = 136/310 (43.87%), Postives = 191/310 (61.61%), Query Frame = 0

Query: 8   TSAVGGFEL------WNEIDESKQWQKGIYSALSASYGLISLIALVLHFSLIFTVFCSVS 67
           +SA+  F L      W +++ES  WQ  I+  L+  YG++S+IA+               
Sbjct: 11  SSAIVAFNLKEGKNWWWDVNESPVWQDRIFHVLAVLYGIVSVIAV--------------- 70

Query: 68  CEIHAMRFQSVVVEDVSGAFMKLYIASYRTLLVSSLVAAFVKVQLIRIQIRVPEFEWTTQ 127
                                                     +QL+RIQ+RVPE+ WTTQ
Sbjct: 71  ------------------------------------------IQLVRIQLRVPEYGWTTQ 130

Query: 128 KGFHLMNFIVNGLRAILFGLYKSVFFIRPKVLEMVVMEIPGLLFFSTYTLLVLFWAEIYH 187
           K FH +NF+VNG+RA++F   +    ++P++L+ ++++IP L FF+TY LLVLFWAEIY+
Sbjct: 131 KVFHFLNFMVNGVRALVFLFRRDAQNMQPEILQHILLDIPSLAFFTTYALLVLFWAEIYY 190

Query: 188 QARSLPIDKLKPTYCIINGVMYVIQICIWIFVMLNQSPGAVIAAKLFFSVVSFSAALGFL 247
           QAR++  D L+P++  IN V+YVIQI +W+ +        VI +K+FF+ VS  AALGFL
Sbjct: 191 QARAVSTDGLRPSFFTINAVVYVIQIALWLVLWWKPVHLMVIISKMFFAGVSLFAALGFL 250

Query: 248 IYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICFSCFFIRCFVLALSAFDKDADLDVLD 307
           +YGGRLF+ML++FP+ES+GR+KKL EVG VTTICF+CF IRC ++   AFD  ADLDVLD
Sbjct: 251 LYGGRLFLMLQRFPVESKGRRKKLQEVGYVTTICFTCFLIRCIMMCFDAFDDAADLDVLD 263

Query: 308 HPILNLIYYM 312
           HPILN IYY+
Sbjct: 311 HPILNFIYYL 263

BLAST of Spg004094 vs. TAIR 10
Match: AT1G14530.2 (Protein of unknown function (DUF1084) )

HSP 1 Score: 255.0 bits (650), Expect = 7.9e-68
Identity = 136/310 (43.87%), Postives = 191/310 (61.61%), Query Frame = 0

Query: 8   TSAVGGFEL------WNEIDESKQWQKGIYSALSASYGLISLIALVLHFSLIFTVFCSVS 67
           +SA+  F L      W +++ES  WQ  I+  L+  YG++S+IA+               
Sbjct: 11  SSAIVAFNLKEGKNWWWDVNESPVWQDRIFHVLAVLYGIVSVIAV--------------- 70

Query: 68  CEIHAMRFQSVVVEDVSGAFMKLYIASYRTLLVSSLVAAFVKVQLIRIQIRVPEFEWTTQ 127
                                                     +QL+RIQ+RVPE+ WTTQ
Sbjct: 71  ------------------------------------------IQLVRIQLRVPEYGWTTQ 130

Query: 128 KGFHLMNFIVNGLRAILFGLYKSVFFIRPKVLEMVVMEIPGLLFFSTYTLLVLFWAEIYH 187
           K FH +NF+VNG+RA++F   +    ++P++L+ ++++IP L FF+TY LLVLFWAEIY+
Sbjct: 131 KVFHFLNFMVNGVRALVFLFRRDAQNMQPEILQHILLDIPSLAFFTTYALLVLFWAEIYY 190

Query: 188 QARSLPIDKLKPTYCIINGVMYVIQICIWIFVMLNQSPGAVIAAKLFFSVVSFSAALGFL 247
           QAR++  D L+P++  IN V+YVIQI +W+ +        VI +K+FF+ VS  AALGFL
Sbjct: 191 QARAVSTDGLRPSFFTINAVVYVIQIALWLVLWWKPVHLMVIISKMFFAGVSLFAALGFL 250

Query: 248 IYGGRLFVMLRQFPIESRGRQKKLYEVGCVTTICFSCFFIRCFVLALSAFDKDADLDVLD 307
           +YGGRLF+ML++FP+ES+GR+KKL EVG VTTICF+CF IRC ++   AFD  ADLDVLD
Sbjct: 251 LYGGRLFLMLQRFPVESKGRRKKLQEVGYVTTICFTCFLIRCIMMCFDAFDDAADLDVLD 263

Query: 308 HPILNLIYYM 312
           HPILN IYY+
Sbjct: 311 HPILNFIYYL 263

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038888866.16.4e-12076.28tobamovirus multiplication protein 1-like isoform X1 [Benincasa hispida][more]
XP_022988878.11.4e-11974.60tobamovirus multiplication protein 1-like [Cucurbita maxima] >XP_022988879.1 tob... [more]
XP_004136876.13.2e-11975.32tobamovirus multiplication protein 1 isoform X1 [Cucumis sativus] >KGN43722.1 hy... [more]
XP_023530045.13.2e-11974.60tobamovirus multiplication protein 1-like [Cucurbita pepo subsp. pepo][more]
XP_022927929.14.2e-11974.28tobamovirus multiplication protein 1-like isoform X3 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q402F41.1e-8253.22Tobamovirus multiplication protein 1 OS=Nicotiana tabacum OX=4097 GN=TOM1 PE=1 S... [more]
Q9FEG21.1e-7951.15Tobamovirus multiplication protein 1 OS=Arabidopsis thaliana OX=3702 GN=TOM1 PE=... [more]
Q9ZUM25.3e-6944.75Tobamovirus multiplication protein 3 OS=Arabidopsis thaliana OX=3702 GN=TOM3 PE=... [more]
Q402F31.6e-6857.08Tobamovirus multiplication protein 3 OS=Nicotiana tabacum OX=4097 GN=TOM3 PE=1 S... [more]
Q948R81.1e-6643.87Protein TOM THREE HOMOLOG 1 OS=Arabidopsis thaliana OX=3702 GN=THH1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JNL16.9e-12074.60tobamovirus multiplication protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A0A0K5Z81.5e-11975.32DUF1084 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G062880 PE=... [more]
A0A6J1EME92.0e-11974.28tobamovirus multiplication protein 1-like isoform X1 OS=Cucurbita moschata OX=36... [more]
A0A6J1EJD72.0e-11974.28tobamovirus multiplication protein 1-like isoform X3 OS=Cucurbita moschata OX=36... [more]
A0A6J1HVA76.1e-11673.31tobamovirus multiplication protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT4G21790.18.2e-8151.15tobamovirus multiplication 1 [more]
AT2G02180.13.8e-7044.75tobamovirus multiplication protein 3 [more]
AT1G14530.17.9e-6843.87Protein of unknown function (DUF1084) [more]
AT1G14530.27.9e-6843.87Protein of unknown function (DUF1084) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009457THH1/TOM1/TOM3 domainPFAMPF06454DUF1084coord: 94..311
e-value: 5.4E-101
score: 337.5
NoneNo IPR availablePANTHERPTHR31142:SF30SUBFAMILY NOT NAMEDcoord: 94..311
coord: 15..51
IPR040226THH1/TOM1/TOM3PANTHERPTHR31142TOBAMOVIRUS MULTIPLICATION PROTEIN 1-LIKE ISOFORM X1coord: 94..311
coord: 15..51

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg004094.1Spg004094.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005774 vacuolar membrane