Spg003691 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg003691
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionVacuolar protein sorting-associated protein 32-like protein 2
Locationscaffold4: 48820906 .. 48825076 (+)
RNA-Seq ExpressionSpg003691
SyntenySpg003691
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATTTATAAACATGTTTAGCCATTTTTCAAATCCCTCTATTAAATCTAGTCATTTTTCTAGTTGTCCCTTTAATTTAAATATATTTTAATTTGTTTGTACATAATGTTTGGGCTTGTCGGTACCTGACCTCGTTCGGCCCATAGTGACGTTCACAGTCCAAGTAAGATTCTGGGCCGATCCATGAAGAGATATCCAGCCCATATTAGGGTTCGGATCGGGCCCGGCCCACATCTCCCATTTAAATTGAATTGGCGAGAGAATAATTGGTCGTTTTGTTCTACGGAAACCAACAAGTACATTGCCGCGTAGAGACGAAACAGCGACGTGAAGAGAAAGGGAACTCCAAATCTTCGTCCACCTTTTTTCTGGAGCGCCTAAGAAGAAAACCTTTCTACTTCAAATTTCTTCACAAATTCTTTCGATTCATTCTCCTTTTTTGGAAATTTGTTTCTGATTCTCATCTTTCCGTCTCAATCTCTCACTGGTTTCTCGATTTCTCCTTTGGTAGGCACCGATTTGCTTTTAAAATCTCATTTTATCGTGTCGAAGTTGATCGGCAGTGCGATTTCTTGAAACTAAGCGTTTTGATGGTTTAATTTTGTTTGTGATTTCGTTGTTGCAGAAGATCGAGAGGCGATTCTTGTTGCTTAGCTTCAACAATGTTTAACAGGCTTTTTGGTAAACCTAAACAAGAAGCCAATGCTCTGGCTACGTTGGATAAGTTGAGCGAGGTTCGTATTCACTTCGTTGTTTTAATGTTTTCGGTATTCTTTTTTATGGAAACGATCTTTTCTTTTTCTCGCTTGTTCTGAACCCTAGCTATATCTCATCGATCTTGATGGATGATTGACTTCTTGATCATTCTTCTTTCCATTGCGTTTTCTAAATGTTAGGTTATAGTAATTGGCTCTCGTTGACGGAGTATTAGTGTTTATGACGTCGTAGTAATTGGTTGTCCTTGACCGAGTATTAGTGTTTATGATGTCATAGTAATTGGTTCTCCTTGTTATTTGGATTTGGTTAACTTATTTTACATTTCTTCTGCCACTGGTAATTGCCGTACTTGAGGTGAAATTTCACTTATGTAAGAAAGGAAAACTGTGTACAACATTGCTATATTTTTCTTCCTTATTGGATATATCGAAGGAATAGATAATTGGAATCACGTCCAAACTGATTGTGATTGTGATTGTGATTGTGATTGCATAGTCTTTTGTAGTCTGACAAAGCTTTTTCTAATTGATACCAATATTTTTGGGCCGTTGTTTTGTCCAGTATTGTTTCCATTTCATCGTCTGTTATTTTCTGTTTAGACACTTGAAATGCTGGAGAAGAAAGAGAATGTTCTTATTAAGAAGGCAGCTGCAGAGGTTGAAAAAGCTAAAGAATTCACAAGAGTGAAGAATAAACGAGGTATGCTCTGAAATTGGGTGATTTCTTCTATGGTGGGATGCTTCTTGGTATTAGTTTTTATTTCTTTTTTGAGCATTTTTTTACGTGTAGTGTGAATAAGATTTAATTTACAACTAGGTTGTTGGCTACGAAGTGTAAATTTGATGGTAGTGCCATACTTGTTTTCTCATGCTTAAAAGTAATAAAACTTTTAATGTAATCTTGGAATGCAAGTTATGCTTAGGTCGTTGGTCGCAATGTGGCACTCTGCAGAACTTTCTACTGCAGTCCTACATCTATTCTTTTGTTCATTGTCGTTATTTCTGATTATTAGAAGGAAAACCTTGTGCTCCATGAATTATTCAACTCTATATCCTCAGTAAGATGTAGCAGTCTCCCTGCTTGTGTTTTTTTTTTTTTTTTGGGGGGGGGGGGGGGAGATAAATCACAAGAGGGGTTTTGCTGGGTTTAGATTCTAGGTCCATCTTTCCCCATTTATTTTTTGTCACACAGGTAGAGAGTAGAGACAGAGTCCTATGTTAATCTCAATACAATTCTTGAGTTATAGAGTGGTAGGGTTTTTCTGTTATATCGGTAAACTTGCTAGTGCTAGATGGGATTTTGCATGTTGGGTCCTTCTGCCTTGTGTATTTTTTGGAGGATGATTTTATCTGGCCTTTTGCTACCAGCGCTTAATACTGATTGTTGGAATTTATCCCGATACTTTTTATAATACGTTTATGAATGGCCAAATGTATCTTTGTTTCTTTCAAATCTTCGTTTGGGCTTTTTATCAATTTTGGATTTTTGTGATTTTCCTTGTTCATATCATCAGTAGTTATGGATATCGCTTTCATATGCAAGTATAATTGTATAGTTCTTTATGTCTATTTTTGTCTGAGCTGTATTTTTGGCGTTTGTTCTGTATGCTTCTTTGGGCTCTATAGTTTGTTCCTTTTCAATCTTCTTATTAATTTATTTTTTTAAAAAATGTTATCTTTGCTATTCCTTATCCTCAAGTCCCTGGTTTACCATGTGTACTTACCTGCCAACCCATTTGGAACGTTTTGTTTAGTGGATATGATTAATAAATAAAAATATTTAGGATTTAAATGTCTCATATATTAAATAGTTCCTGAATAAATTAATGATCTAGAATTATTTAATATCAATCAGAAATTAAAATTAAAATTGTTATTTTTATCTTGAATTTTTGAATAAAACCGTCTGTCTTCTGTTTGTAAAATTCCATTCAGTCATTAGAATTTGTTTGCAGCGGCCATACAATGTCTGAAGAGGAAAAGGCTTTATGAACAGCAAATAGAGCAGCTTGGAAATTTCCAATTGCGTATCCATGATCAGGTGAGCTGGTGTTGTTATTCACTGAATGATGAGACATCTATTTCTTTTACTTTCAGTTAGCTCAGCTTTGTGGAAATGAACTGTTTTATTTATTTACTGCAGATGATTATGTTAGAAGGTGCAAAAGCTACGACGGAGACTGTGGATGCTTTGAGAACAGGAGCATCTGCAATGAAGGCCATGCAGAAAGCAACGTATGCCTAATTTCATTTGACTTGCTCTCTTTCTCTGCCTTTGTGGGTTATAACCTTTCAGGATTTTCTTCTTGCTGGGTAATTAGATATTCTTAGATATATAGTTTCAAGGGTTTTATGAAAAATTTTAACAAAATTAAGCTTGTGTTTTATTTCTTTCACTGAGATTGTGAAATTTTAGATCTGTACTTCATTTGTAGAAACTAGCCTCTGATTATTTGAAACTTTCATCTTCAGAAATATAGATGATGTGGACAAGACAATGGATGAGATCAATGAGCAGACTGAGAATATGAAACAGATACAGGAAGCACTTTCAGCTCCAATTGGTGCTGCGGCTGATTTCGATGAGGTTGGTTGATCTTTGGCTTGTTTCTTGACCTTTGCTTGTATCTCATATTTCATCTATTAACAATGGTGTGTAATCAGGATGAATTAGAGGCCGAACTTGAGGAGCTGGAAAGTGCTGAGTTGGAAGAGCAACTACTCCAGCCAGCGTCAACAGCTCCTGCTGCACCTGTTTCGGTCCCATCAGGCCGAGTACCTGCACGTCCTGCTCCACAGAAGAGAACTGCTGAAGAAGATGAGCTGGCAGCTCTGCAAGCAGAGATGGCCCTCTAACCAGGTTTGTTTATCTGAGCTGAATGAGTTTCAATTAGCTTTGCATAAATGTCAATCTTAAAGACCAACAAATAATTGAACAATCCTTTTAAGATTTTTATACTTGAATTGAGTTTATATGCAATGGTTTGTTGTAACTGAATTTAGATTGGACATTTTTCAGGTGGTGTAAACAAGTATTATCTCCTGGGAGAGTGCATCCGAGAAATCTTCTGATGAGTGTTTAAAATTCTATACGAATTATTATTCCCTGCCGCAAGACGATGTATATAAAGGTGGCAAAATTATTGGGATGTTCATTTTAAAATCTTCTATTTACTTTTACTTTTATTTTGCAGAGATTGCAAAACAACATTGACATTTTTTTTTTACTTTCGTGCACCTCGGGAAATTTAGGTATATTTGAGCAATTATCTTTTCTTGATTTTTATAATAAAAGTGGCTTATTCGTTTAGATGGAATGAAACAATAAATAAGTCTTAAGTTTAGGTAGTTCTTACTGTCCGATCGTTTTAATACAAGTGTCAGCCTTATTTGTCTACCGTTTATCATCTGTTAAATTAAAGGAATTGAATTTGTATGAAAATACAGTAGCCAATGC

mRNA sequence

ATGTTTAACAGGCTTTTTGGTAAACCTAAACAAGAAGCCAATGCTCTGGCTACGTTGGATAAGTTGAGCGAGACACTTGAAATGCTGGAGAAGAAAGAGAATGTTCTTATTAAGAAGGCAGCTGCAGAGGTTGAAAAAGCTAAAGAATTCACAAGAGTGAAGAATAAACGAGCGGCCATACAATGTCTGAAGAGGAAAAGGCTTTATGAACAGCAAATAGAGCAGCTTGGAAATTTCCAATTGCGTATCCATGATCAGATGATTATGTTAGAAGGTGCAAAAGCTACGACGGAGACTGTGGATGCTTTGAGAACAGGAGCATCTGCAATGAAGGCCATGCAGAAAGCAACAAATATAGATGATGTGGACAAGACAATGGATGAGATCAATGAGCAGACTGAGAATATGAAACAGATACAGGAAGCACTTTCAGCTCCAATTGGTGCTGCGGCTGATTTCGATGAGGATGAATTAGAGGCCGAACTTGAGGAGCTGGAAAGTGCTGAGTTGGAAGAGCAACTACTCCAGCCAGCGTCAACAGCTCCTGCTGCACCTGTTTCGGTCCCATCAGGCCGAGTACCTGCACGTCCTGCTCCACAGAAGAGAACTGCTGAAGAAGATGAGCTGGCAGCTCTGCAAGCAGAGATGGCCCTCTAA

Coding sequence (CDS)

ATGTTTAACAGGCTTTTTGGTAAACCTAAACAAGAAGCCAATGCTCTGGCTACGTTGGATAAGTTGAGCGAGACACTTGAAATGCTGGAGAAGAAAGAGAATGTTCTTATTAAGAAGGCAGCTGCAGAGGTTGAAAAAGCTAAAGAATTCACAAGAGTGAAGAATAAACGAGCGGCCATACAATGTCTGAAGAGGAAAAGGCTTTATGAACAGCAAATAGAGCAGCTTGGAAATTTCCAATTGCGTATCCATGATCAGATGATTATGTTAGAAGGTGCAAAAGCTACGACGGAGACTGTGGATGCTTTGAGAACAGGAGCATCTGCAATGAAGGCCATGCAGAAAGCAACAAATATAGATGATGTGGACAAGACAATGGATGAGATCAATGAGCAGACTGAGAATATGAAACAGATACAGGAAGCACTTTCAGCTCCAATTGGTGCTGCGGCTGATTTCGATGAGGATGAATTAGAGGCCGAACTTGAGGAGCTGGAAAGTGCTGAGTTGGAAGAGCAACTACTCCAGCCAGCGTCAACAGCTCCTGCTGCACCTGTTTCGGTCCCATCAGGCCGAGTACCTGCACGTCCTGCTCCACAGAAGAGAACTGCTGAAGAAGATGAGCTGGCAGCTCTGCAAGCAGAGATGGCCCTCTAA

Protein sequence

MFNRLFGKPKQEANALATLDKLSETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAIQCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEELESAELEEQLLQPASTAPAAPVSVPSGRVPARPAPQKRTAEEDELAALQAEMAL
Homology
BLAST of Spg003691 vs. NCBI nr
Match: XP_008459153.1 (PREDICTED: vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis melo] >XP_008459161.1 PREDICTED: vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis melo] >XP_008459166.1 PREDICTED: vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis melo] >XP_038889616.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Benincasa hispida] >XP_038889617.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Benincasa hispida] >KAA0061506.1 vacuolar protein sorting-associated protein 32-like protein 2 [Cucumis melo var. makuwa] >TYK10767.1 vacuolar protein sorting-associated protein 32-like protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 385.6 bits (989), Expect = 2.9e-103
Identity = 213/218 (97.71%), Postives = 218/218 (100.00%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLDKLSETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           MFNRLFGKPKQEANALATL+KLSETLEMLEKKENVL+KKAAAEVE+AKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELE+LESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180

Query: 181 APAAPVSVPSGRVPARPAPQKRTAEEDELAALQAEMAL 219
           APAAPVSVP+GRVPARPAPQKRTAEEDELAALQAEMAL
Sbjct: 181 APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 218

BLAST of Spg003691 vs. NCBI nr
Match: XP_022937864.1 (vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita moschata] >XP_022937865.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita moschata] >XP_022937866.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita moschata] >XP_022937867.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita moschata] >XP_023536810.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita pepo subsp. pepo] >XP_023536811.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita pepo subsp. pepo] >XP_023536812.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita pepo subsp. pepo] >KAG7021320.1 Vacuolar protein sorting-associated protein 32-like 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 382.5 bits (981), Expect = 2.5e-102
Identity = 212/218 (97.25%), Postives = 214/218 (98.17%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLDKLSETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M NRLFGKPKQEANALATLDKL+ETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI
Sbjct: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALS PIGAAADFDEDELEAELEELESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 APAAPVSVPSGRVPARPAPQKRTAEEDELAALQAEMAL 219
           APA PVSVPSGRVPARP PQKRTAEEDELAALQAEMAL
Sbjct: 181 APAGPVSVPSGRVPARPVPQKRTAEEDELAALQAEMAL 218

BLAST of Spg003691 vs. NCBI nr
Match: KAG7030473.1 (Vacuolar protein sorting-associated protein 32-like 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 382.5 bits (981), Expect = 2.5e-102
Identity = 210/218 (96.33%), Postives = 215/218 (98.62%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLDKLSETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           MFNRLFGKPKQEANALATL+KLSETLEMLEKKENVL+KKA+AEVEKAKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALS PIGAAADFDEDELEAELEELESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 APAAPVSVPSGRVPARPAPQKRTAEEDELAALQAEMAL 219
            PAAPVS+PSGRVPARP PQKRTAEEDELAALQAEMAL
Sbjct: 181 TPAAPVSIPSGRVPARPVPQKRTAEEDELAALQAEMAL 218

BLAST of Spg003691 vs. NCBI nr
Match: XP_004141038.1 (vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis sativus] >XP_011648616.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis sativus] >XP_031736822.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis sativus] >KGN60554.1 hypothetical protein Csa_019460 [Cucumis sativus])

HSP 1 Score: 382.5 bits (981), Expect = 2.5e-102
Identity = 212/218 (97.25%), Postives = 216/218 (99.08%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLDKLSETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           MFNRLFGKPKQEANALATL+KLSETLEMLEKKENVL+KKAAAEVE+AKEFTR KNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELE+LESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180

Query: 181 APAAPVSVPSGRVPARPAPQKRTAEEDELAALQAEMAL 219
           APAAPVSVP GRVPARPAPQKRTAEEDELAALQAEMAL
Sbjct: 181 APAAPVSVPGGRVPARPAPQKRTAEEDELAALQAEMAL 218

BLAST of Spg003691 vs. NCBI nr
Match: XP_023545615.1 (vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023545616.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023545619.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023545620.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023547008.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023547009.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023547010.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023547011.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 380.9 bits (977), Expect = 7.1e-102
Identity = 210/218 (96.33%), Postives = 214/218 (98.17%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLDKLSETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           MFNRLFGKPKQEANALATL+KLSETLEMLEKKENVL+KKA+AEVEKAKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALS PIGAAADFDEDELEAELEELESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 APAAPVSVPSGRVPARPAPQKRTAEEDELAALQAEMAL 219
            PAAPVSVPSGRVPARP PQKR AEEDELAALQAEMAL
Sbjct: 181 TPAAPVSVPSGRVPARPVPQKRNAEEDELAALQAEMAL 218

BLAST of Spg003691 vs. ExPASy Swiss-Prot
Match: Q9SZE4 (Vacuolar protein sorting-associated protein 32 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=VPS32.2 PE=1 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 2.8e-93
Identity = 191/219 (87.21%), Postives = 203/219 (92.69%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLDKLSETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M NRLFGKPKQEANAL TLDKL+ETLEMLEKKE VL+KKA AEVEKAKE++R KNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYE Q+EQLGNFQLRIHDQMIMLEGAKATTETVDALR+GASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEAL+ P+GAAADFDEDEL AEL+ELES ELE QLLQPA+T
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATT 180

Query: 181 APAAP-VSVPSGRVPARPAPQKRTAEEDELAALQAEMAL 219
           AP  P V VP+GR PARP PQKRTAEE+ELAALQAEMAL
Sbjct: 181 APPLPSVPVPAGRQPARPVPQKRTAEEEELAALQAEMAL 219

BLAST of Spg003691 vs. ExPASy Swiss-Prot
Match: O82197 (Vacuolar protein sorting-associated protein 32 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=VPS32.1 PE=1 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 3.3e-86
Identity = 184/217 (84.79%), Postives = 193/217 (88.94%), Query Frame = 0

Query: 3   NRLFGKPKQEANALATLDKLSETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAIQC 62
           NRLFGKPKQE + L TLDKL+ETLEMLEKKENVL+KKA  EVEKAKEF+R KNKRAAIQC
Sbjct: 4   NRLFGKPKQETSTLQTLDKLNETLEMLEKKENVLLKKATGEVEKAKEFSRAKNKRAAIQC 63

Query: 63  LKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV 122
           LKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV
Sbjct: 64  LKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV 123

Query: 123 DKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEELESAELEEQLLQPASTAP 182
           DKTMDEINEQTENMKQIQEALSAP G A DFDEDELEAEL+ELE AELEEQLLQP     
Sbjct: 124 DKTMDEINEQTENMKQIQEALSAPFG-ANDFDEDELEAELDELEGAELEEQLLQP----- 183

Query: 183 AAPVSVPSGRVPAR-PAPQKRTAEEDELAALQAEMAL 219
             P+ VP G  PAR PA ++ TAEEDELAALQAEMAL
Sbjct: 184 -VPIHVPQGNKPARAPAQKQPTAEEDELAALQAEMAL 213

BLAST of Spg003691 vs. ExPASy Swiss-Prot
Match: Q7ZVC4 (Charged multivesicular body protein 4b OS=Danio rerio OX=7955 GN=chmp4b PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 7.1e-28
Identity = 90/217 (41.47%), Postives = 127/217 (58.53%), Query Frame = 0

Query: 1   MFNRLF---GKPKQEANALATLDKLSETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKR 60
           +F ++F   GK  + A+    + +L ET EML KK+  L KK   E+  AK+    KNKR
Sbjct: 3   LFGKMFGSGGKGGKSASPQEAIQRLRETEEMLTKKQEFLEKKIEQELVTAKK-NGTKNKR 62

Query: 61  AAIQCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKAT 120
           AA+Q LKRK+ YE+Q+ Q+      I  Q   LE A   TE +  +   A AMKA     
Sbjct: 63  AALQALKRKKRYEKQLAQIDGTLSTIEFQREALENAHTNTEVIKNMGYAAKAMKAAHDNM 122

Query: 121 NIDDVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEELESAELEEQLLQP 180
           +ID VD+ M +I EQ E  ++I +A+S P+G   +FDEDEL AELEELE  EL++ LL+ 
Sbjct: 123 DIDKVDELMQDIIEQQELAQEISDAISKPVGFGEEFDEDELLAELEELEQEELDKNLLEI 182

Query: 181 ASTAPAAPVSVPSGRVPARPAPQKRTAEEDELAALQA 215
               P    +VPS  +P+RPA +K   +ED++  L+A
Sbjct: 183 GDNVPLP--NVPSTSLPSRPAKKKEEEDEDDMKDLEA 216

BLAST of Spg003691 vs. ExPASy Swiss-Prot
Match: Q6IQ73 (Charged multivesicular body protein 4c OS=Danio rerio OX=7955 GN=chmp4c PE=2 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.5e-25
Identity = 90/212 (42.45%), Postives = 121/212 (57.08%), Query Frame = 0

Query: 1   MFNRLF---GKPKQEANALATLDKLSETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKR 60
           +F +LF   GK  +       + KL ET EML KK++ L KK  AE+  AK+    KNKR
Sbjct: 3   VFGKLFGGGGKGGKGPTPQEAIQKLRETEEMLAKKQDFLEKKIDAELLIAKK-NGTKNKR 62

Query: 61  AAIQCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKAT 120
           AA+Q LKRK+ YE+Q+ Q+      I  Q   LE A   TE +  +   A AMK   +  
Sbjct: 63  AALQALKRKKRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGFAAKAMKTAHENM 122

Query: 121 NIDDVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEELESAELEEQLLQP 180
           +ID VD  M +I EQ E  ++I +A+S P+G   +FDEDEL AELEELE  EL++ LLQ 
Sbjct: 123 DIDKVDDLMQDITEQQELAQEISDAISRPVGFGEEFDEDELMAELEELEQEELDKDLLQI 182

Query: 181 ASTAPAAPVSVPSGRVPARPA-PQKRTAEEDE 209
           +        +VPS  +P + A  QK+  EEDE
Sbjct: 183 SGPEDVPLPNVPSNPLPKKTAVAQKKRQEEDE 213

BLAST of Spg003691 vs. ExPASy Swiss-Prot
Match: Q5ZHP5 (Charged multivesicular body protein 4b OS=Gallus gallus OX=9031 GN=CHMP4B PE=2 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 4.3e-25
Identity = 85/208 (40.87%), Postives = 119/208 (57.21%), Query Frame = 0

Query: 7   GKPKQEANALATLDKLSETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAIQCLKRK 66
           G   QEA     + +L +T EML KK+  L KK   E+  A++    KNKRAA+Q LKRK
Sbjct: 21  GPSPQEA-----IQRLRDTEEMLSKKQEFLEKKIEQELAAARKH-GTKNKRAALQALKRK 80

Query: 67  RLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDVDKTM 126
           + YE+Q+ Q+      I  Q   LE A   TE +  +   A AMKA     +ID VD+ M
Sbjct: 81  KRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGFAAKAMKAAHDNMDIDKVDELM 140

Query: 127 DEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEELESAELEEQLLQPASTAPAAPV 186
            +I EQ E   +I  A+S P+G   +FDEDEL AELEELE  EL++ LL+ +        
Sbjct: 141 QDIAEQQELADEISTAISKPVGFGEEFDEDELMAELEELEQEELDKNLLEISGPETVPLP 200

Query: 187 SVPSGRVPARPAPQKRTAEEDELAALQA 215
           +VPS  +P++PA +K   E+D++  L+A
Sbjct: 201 NVPSISIPSKPAKKKEEEEDDDMKELEA 222

BLAST of Spg003691 vs. ExPASy TrEMBL
Match: A0A1S3CAQ7 (vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucumis melo OX=3656 GN=LOC103498351 PE=4 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 1.4e-103
Identity = 213/218 (97.71%), Postives = 218/218 (100.00%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLDKLSETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           MFNRLFGKPKQEANALATL+KLSETLEMLEKKENVL+KKAAAEVE+AKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELE+LESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180

Query: 181 APAAPVSVPSGRVPARPAPQKRTAEEDELAALQAEMAL 219
           APAAPVSVP+GRVPARPAPQKRTAEEDELAALQAEMAL
Sbjct: 181 APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 218

BLAST of Spg003691 vs. ExPASy TrEMBL
Match: A0A5A7V4V7 (Vacuolar protein sorting-associated protein 32-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00800 PE=4 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 1.4e-103
Identity = 213/218 (97.71%), Postives = 218/218 (100.00%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLDKLSETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           MFNRLFGKPKQEANALATL+KLSETLEMLEKKENVL+KKAAAEVE+AKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELE+LESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180

Query: 181 APAAPVSVPSGRVPARPAPQKRTAEEDELAALQAEMAL 219
           APAAPVSVP+GRVPARPAPQKRTAEEDELAALQAEMAL
Sbjct: 181 APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 218

BLAST of Spg003691 vs. ExPASy TrEMBL
Match: A0A6J1FBK1 (vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucurbita moschata OX=3662 GN=LOC111444128 PE=4 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 1.2e-102
Identity = 212/218 (97.25%), Postives = 214/218 (98.17%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLDKLSETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M NRLFGKPKQEANALATLDKL+ETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI
Sbjct: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALS PIGAAADFDEDELEAELEELESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 APAAPVSVPSGRVPARPAPQKRTAEEDELAALQAEMAL 219
           APA PVSVPSGRVPARP PQKRTAEEDELAALQAEMAL
Sbjct: 181 APAGPVSVPSGRVPARPVPQKRTAEEDELAALQAEMAL 218

BLAST of Spg003691 vs. ExPASy TrEMBL
Match: A0A0A0LHT5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G000620 PE=4 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 1.2e-102
Identity = 212/218 (97.25%), Postives = 216/218 (99.08%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLDKLSETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           MFNRLFGKPKQEANALATL+KLSETLEMLEKKENVL+KKAAAEVE+AKEFTR KNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRGKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELE+LESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180

Query: 181 APAAPVSVPSGRVPARPAPQKRTAEEDELAALQAEMAL 219
           APAAPVSVP GRVPARPAPQKRTAEEDELAALQAEMAL
Sbjct: 181 APAAPVSVPGGRVPARPAPQKRTAEEDELAALQAEMAL 218

BLAST of Spg003691 vs. ExPASy TrEMBL
Match: A0A6J1G2S3 (vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita moschata OX=3662 GN=LOC111450291 PE=4 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 7.7e-102
Identity = 208/218 (95.41%), Postives = 214/218 (98.17%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLDKLSETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           MFNRLFGKPKQEANALATL+KLSETLEMLEKKENVL+KKA+AEVEKAKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMD+INEQTENMKQIQEALS PIGAAADFDEDELEAELEELESAELEEQLLQPAST
Sbjct: 121 DVDKTMDDINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 APAAPVSVPSGRVPARPAPQKRTAEEDELAALQAEMAL 219
            PAAPVS+PSGRVPARP PQKRTAEEDEL ALQAEMAL
Sbjct: 181 TPAAPVSIPSGRVPARPVPQKRTAEEDELTALQAEMAL 218

BLAST of Spg003691 vs. TAIR 10
Match: AT4G29160.1 (SNF7 family protein )

HSP 1 Score: 342.8 bits (878), Expect = 2.0e-94
Identity = 191/219 (87.21%), Postives = 203/219 (92.69%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLDKLSETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M NRLFGKPKQEANAL TLDKL+ETLEMLEKKE VL+KKA AEVEKAKE++R KNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYE Q+EQLGNFQLRIHDQMIMLEGAKATTETVDALR+GASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEAL+ P+GAAADFDEDEL AEL+ELES ELE QLLQPA+T
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATT 180

Query: 181 APAAP-VSVPSGRVPARPAPQKRTAEEDELAALQAEMAL 219
           AP  P V VP+GR PARP PQKRTAEE+ELAALQAEMAL
Sbjct: 181 APPLPSVPVPAGRQPARPVPQKRTAEEEELAALQAEMAL 219

BLAST of Spg003691 vs. TAIR 10
Match: AT4G29160.3 (SNF7 family protein )

HSP 1 Score: 342.8 bits (878), Expect = 2.0e-94
Identity = 191/219 (87.21%), Postives = 203/219 (92.69%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLDKLSETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M NRLFGKPKQEANAL TLDKL+ETLEMLEKKE VL+KKA AEVEKAKE++R KNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYE Q+EQLGNFQLRIHDQMIMLEGAKATTETVDALR+GASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEAL+ P+GAAADFDEDEL AEL+ELES ELE QLLQPA+T
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATT 180

Query: 181 APAAP-VSVPSGRVPARPAPQKRTAEEDELAALQAEMAL 219
           AP  P V VP+GR PARP PQKRTAEE+ELAALQAEMAL
Sbjct: 181 APPLPSVPVPAGRQPARPVPQKRTAEEEELAALQAEMAL 219

BLAST of Spg003691 vs. TAIR 10
Match: AT2G19830.1 (SNF7 family protein )

HSP 1 Score: 319.3 bits (817), Expect = 2.4e-87
Identity = 184/217 (84.79%), Postives = 193/217 (88.94%), Query Frame = 0

Query: 3   NRLFGKPKQEANALATLDKLSETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAIQC 62
           NRLFGKPKQE + L TLDKL+ETLEMLEKKENVL+KKA  EVEKAKEF+R KNKRAAIQC
Sbjct: 4   NRLFGKPKQETSTLQTLDKLNETLEMLEKKENVLLKKATGEVEKAKEFSRAKNKRAAIQC 63

Query: 63  LKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV 122
           LKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV
Sbjct: 64  LKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDV 123

Query: 123 DKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEELESAELEEQLLQPASTAP 182
           DKTMDEINEQTENMKQIQEALSAP G A DFDEDELEAEL+ELE AELEEQLLQP     
Sbjct: 124 DKTMDEINEQTENMKQIQEALSAPFG-ANDFDEDELEAELDELEGAELEEQLLQP----- 183

Query: 183 AAPVSVPSGRVPAR-PAPQKRTAEEDELAALQAEMAL 219
             P+ VP G  PAR PA ++ TAEEDELAALQAEMAL
Sbjct: 184 -VPIHVPQGNKPARAPAQKQPTAEEDELAALQAEMAL 213

BLAST of Spg003691 vs. TAIR 10
Match: AT4G29160.2 (SNF7 family protein )

HSP 1 Score: 297.0 bits (759), Expect = 1.3e-80
Identity = 167/192 (86.98%), Postives = 178/192 (92.71%), Query Frame = 0

Query: 28  MLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAIQCLKRKRLYEQQIEQLGNFQLRIHDQM 87
           MLEKKE VL+KKA AEVEKAKE++R KNKRAAIQCLKRKRLYE Q+EQLGNFQLRIHDQM
Sbjct: 1   MLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAIQCLKRKRLYEGQVEQLGNFQLRIHDQM 60

Query: 88  IMLEGAKATTETVDALRTGASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALSAPI 147
           IMLEGAKATTETVDALR+GASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEAL+ P+
Sbjct: 61  IMLEGAKATTETVDALRSGASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALATPM 120

Query: 148 GAAADFDEDELEAELEELESAELEEQLLQPASTAPAAP-VSVPSGRVPARPAPQKRTAEE 207
           GAAADFDEDEL AEL+ELES ELE QLLQPA+TAP  P V VP+GR PARP PQKRTAEE
Sbjct: 121 GAAADFDEDELAAELDELESEELESQLLQPATTAPPLPSVPVPAGRQPARPVPQKRTAEE 180

Query: 208 DELAALQAEMAL 219
           +ELAALQAEMAL
Sbjct: 181 EELAALQAEMAL 192

BLAST of Spg003691 vs. TAIR 10
Match: AT5G09260.1 (vacuolar protein sorting-associated protein 20.2 )

HSP 1 Score: 57.0 bits (136), Expect = 2.2e-08
Identity = 60/199 (30.15%), Postives = 95/199 (47.74%), Query Frame = 0

Query: 1   MFNRLFGKPKQEANALATLDKLSETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M N    KPK      A L  L      L + +  L K   AE + A++  R K K  A+
Sbjct: 1   MGNLFVKKPKITEVDRAIL-SLKTQRRKLGQYQQQLEKVIEAEKQAARDLIREKRKDRAL 60

Query: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
             LK+KR  E+ ++Q+  + + +  Q+  +E         ++L+ G +A+KA+Q   N+D
Sbjct: 61  LALKKKRTQEELLKQVDQWLINVEQQLADIELTSKQKAVFESLKQGNNAIKAIQSEVNLD 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADF-DEDELEAELEELESAELEEQLLQPAS 180
           DV K MD+    T   K  Q+ LSA +G      DE+E+ AE + LES  + E + +  +
Sbjct: 121 DVQKLMDD----TAEAKAYQDELSAILGEKLSAEDEEEILAEFDNLESLLIVEDMPEVPT 180

Query: 181 T--APAAPVSVPSGRVPAR 197
           T   P  P  +    VP +
Sbjct: 181 TELMPEEPEKMDLPDVPTK 194

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008459153.12.9e-10397.71PREDICTED: vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis mel... [more]
XP_022937864.12.5e-10297.25vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita moschata] >X... [more]
KAG7030473.12.5e-10296.33Vacuolar protein sorting-associated protein 32-like 2 [Cucurbita argyrosperma su... [more]
XP_004141038.12.5e-10297.25vacuolar protein sorting-associated protein 32 homolog 2 [Cucumis sativus] >XP_0... [more]
XP_023545615.17.1e-10296.33vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo su... [more]
Match NameE-valueIdentityDescription
Q9SZE42.8e-9387.21Vacuolar protein sorting-associated protein 32 homolog 2 OS=Arabidopsis thaliana... [more]
O821973.3e-8684.79Vacuolar protein sorting-associated protein 32 homolog 1 OS=Arabidopsis thaliana... [more]
Q7ZVC47.1e-2841.47Charged multivesicular body protein 4b OS=Danio rerio OX=7955 GN=chmp4b PE=2 SV=... [more]
Q6IQ731.5e-2542.45Charged multivesicular body protein 4c OS=Danio rerio OX=7955 GN=chmp4c PE=2 SV=... [more]
Q5ZHP54.3e-2540.87Charged multivesicular body protein 4b OS=Gallus gallus OX=9031 GN=CHMP4B PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A1S3CAQ71.4e-10397.71vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucumis melo OX=3656... [more]
A0A5A7V4V71.4e-10397.71Vacuolar protein sorting-associated protein 32-like protein 2 OS=Cucumis melo va... [more]
A0A6J1FBK11.2e-10297.25vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucurbita moschata O... [more]
A0A0A0LHT51.2e-10297.25Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G000620 PE=4 SV=1[more]
A0A6J1G2S37.7e-10295.41vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita mosch... [more]
Match NameE-valueIdentityDescription
AT4G29160.12.0e-9487.21SNF7 family protein [more]
AT4G29160.32.0e-9487.21SNF7 family protein [more]
AT2G19830.12.4e-8784.79SNF7 family protein [more]
AT4G29160.21.3e-8086.98SNF7 family protein [more]
AT5G09260.12.2e-0830.15vacuolar protein sorting-associated protein 20.2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 151..175
NoneNo IPR availableCOILSCoilCoilcoord: 122..142
NoneNo IPR availableCOILSCoilCoilcoord: 12..39
NoneNo IPR availableGENE3D6.10.250.1710coord: 117..171
e-value: 5.5E-9
score: 37.8
NoneNo IPR availableGENE3D1.10.287.1060coord: 14..91
e-value: 6.5E-22
score: 79.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 173..218
NoneNo IPR availablePANTHERPTHR22761CHARGED MULTIVESICULAR BODY PROTEINcoord: 1..218
NoneNo IPR availablePANTHERPTHR22761:SF63VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 32 HOMOLOG 1coord: 1..218
IPR005024Snf7 familyPFAMPF03357Snf7coord: 20..185
e-value: 7.1E-46
score: 156.0

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg003691.1Spg003691.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway
biological_process GO:0006900 vesicle budding from membrane
biological_process GO:0007034 vacuolar transport
cellular_component GO:0009898 cytoplasmic side of plasma membrane
cellular_component GO:0000815 ESCRT III complex
cellular_component GO:0005771 multivesicular body