Spg003582 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg003582
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	C2 domain-containing protein
Location	scaffold4: 44411593 .. 44412099 (-)
RNA-Seq Expression	Spg003582
Synteny	Spg003582

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGATGGCGACGGATTACAGAGTATTCGATCTAAACGTTACGTGCGCCGAAGGAATCCAAGGAGTCCCAAGTTTCGTCGATTTTTTCCACAAATGGAAGGTCTACGCGGTGGTTTCCGTCGCCGGAGCCTCGCCGGCCGGCCACCGAGAAACGCCGGTGGCGAAGTACTTCGGTCGAAATCCGGTGTGGAATTTTCCGATGAGGTTTTATTTCGAGGAATCGGAGCTGCGAGAAAATGCCCTAACAGCTCTGAGGATTCAGCTGAAACGAAAGCGAAGTTTTGGAGAAGATGAAGACATTGCCGAAGCTTTAGTTCCGTTGAAGAATCTTTTTCATGGCAATGAGAATGTGACAAATTACACTTCATCGTTGTTGCTCTCGCCGTCCGGCGGCCGTCGTGGTTTCGTGTATTTTTTGTATTACTTTAGTCAGATTCATCGTGCCACCGCCGGACTTTTGGTCGATAGGACATATCAATTATCGTTGAACTATACTCACTTTTGA

mRNA sequence

ATGGCGATGGCGACGGATTACAGAGTATTCGATCTAAACGTTACGTGCGCCGAAGGAATCCAAGGAGTCCCAAGTTTCGTCGATTTTTTCCACAAATGGAAGGTCTACGCGGTGGTTTCCGTCGCCGGAGCCTCGCCGGCCGGCCACCGAGAAACGCCGGTGGCGAAGTACTTCGGTCGAAATCCGGTGTGGAATTTTCCGATGAGGTTTTATTTCGAGGAATCGGAGCTGCGAGAAAATGCCCTAACAGCTCTGAGGATTCAGCTGAAACGAAAGCGAAGTTTTGGAGAAGATGAAGACATTGCCGAAGCTTTAGTTCCGTTGAAGAATCTTTTTCATGGCAATGAGAATGTGACAAATTACACTTCATCGTTGTTGCTCTCGCCGTCCGGCGGCCGTCGTGGTTTCGTGTATTTTTTGTATTACTTTAGTCAGATTCATCGTGCCACCGCCGGACTTTTGGTCGATAGGACATATCAATTATCGTTGAACTATACTCACTTTTGA

Coding sequence (CDS)

ATGGCGATGGCGACGGATTACAGAGTATTCGATCTAAACGTTACGTGCGCCGAAGGAATCCAAGGAGTCCCAAGTTTCGTCGATTTTTTCCACAAATGGAAGGTCTACGCGGTGGTTTCCGTCGCCGGAGCCTCGCCGGCCGGCCACCGAGAAACGCCGGTGGCGAAGTACTTCGGTCGAAATCCGGTGTGGAATTTTCCGATGAGGTTTTATTTCGAGGAATCGGAGCTGCGAGAAAATGCCCTAACAGCTCTGAGGATTCAGCTGAAACGAAAGCGAAGTTTTGGAGAAGATGAAGACATTGCCGAAGCTTTAGTTCCGTTGAAGAATCTTTTTCATGGCAATGAGAATGTGACAAATTACACTTCATCGTTGTTGCTCTCGCCGTCCGGCGGCCGTCGTGGTTTCGTGTATTTTTTGTATTACTTTAGTCAGATTCATCGTGCCACCGCCGGACTTTTGGTCGATAGGACATATCAATTATCGTTGAACTATACTCACTTTTGA

Protein sequence

MAMATDYRVFDLNVTCAEGIQGVPSFVDFFHKWKVYAVVSVAGASPAGHRETPVAKYFGRNPVWNFPMRFYFEESELRENALTALRIQLKRKRSFGEDEDIAEALVPLKNLFHGNENVTNYTSSLLLSPSGGRRGFVYFLYYFSQIHRATAGLLVDRTYQLSLNYTHF

Homology

[Close]

BLAST of Spg003582 vs. NCBI nr
Match: XP_022938307.1 (protein SRC2 [Cucurbita moschata] >KAG7021095.1 Protein SRC2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 205.7 bits (522), Expect = 3.2e-49
Identity = 106/148 (71.62%), Postives = 122/148 (82.43%), Query Frame = 0


Query: 6   DYRVFDLNVTCAEGIQGVPSFVDFFHKWKVYAVVSVAGASPAGHRETPVAKYFGRNPVWN 65
           DYR F +NVTC EGI+GV SFV+FFHK KVYA VS+ G   A ++ETP+AKYFG NPVWN
Sbjct: 2   DYRAFKVNVTCVEGIEGVLSFVNFFHKSKVYAAVSITGDLLA-NQETPLAKYFGENPVWN 61

Query: 66  FPMRFYFEESELRENALTALRIQLKRKRSFGEDEDIAEALVPLKNLFHGNENV--TNYTS 125
           +PM+FY +ES+L+ENALT LRIQL+RKR FG DE IAEALVPLKNLF GN+NV   NY S
Sbjct: 62  WPMKFYLKESKLQENALT-LRIQLRRKRIFGGDEYIAEALVPLKNLFDGNKNVMDENYCS 121

Query: 126 SLLLSPSGGRRGFVYFLYYFSQIHRATA 152
           S LLSPSGGRRGFVYF YYFS+ + ATA
Sbjct: 122 SWLLSPSGGRRGFVYFSYYFSRTYAATA 147

[Close]

BLAST of Spg003582 vs. NCBI nr
Match: KAG6586252.1 (Protein SRC2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 205.7 bits (522), Expect = 3.2e-49
Identity = 106/148 (71.62%), Postives = 122/148 (82.43%), Query Frame = 0


Query: 6   DYRVFDLNVTCAEGIQGVPSFVDFFHKWKVYAVVSVAGASPAGHRETPVAKYFGRNPVWN 65
           DYR F +NVTC EGI+GV SFV+FFHK KVYA VS+ G   A ++ETP+AKYFG NPVWN
Sbjct: 2   DYRAFKVNVTCVEGIEGVLSFVNFFHKSKVYAAVSITGDLLA-NQETPLAKYFGENPVWN 61

Query: 66  FPMRFYFEESELRENALTALRIQLKRKRSFGEDEDIAEALVPLKNLFHGNENV--TNYTS 125
           +PM+FY +ES+L+ENALT LRIQL+RKR FG DE IAEALVPLKNLF GN+NV   NY S
Sbjct: 62  WPMKFYLKESKLQENALT-LRIQLRRKRIFGGDEYIAEALVPLKNLFDGNKNVMDENYCS 121

Query: 126 SLLLSPSGGRRGFVYFLYYFSQIHRATA 152
           S LLSPSGGRRGFVYF YYFS+ + ATA
Sbjct: 122 SWLLSPSGGRRGFVYFSYYFSRTYAATA 147

[Close]

BLAST of Spg003582 vs. NCBI nr
Match: XP_031736682.1 (protein SRC2 homolog [Cucumis sativus] >KGN60949.1 hypothetical protein Csa_021167 [Cucumis sativus])

HSP 1 Score: 184.5 bits (467), Expect = 7.6e-43
Identity = 97/151 (64.24%), Postives = 121/151 (80.13%), Query Frame = 0


Query: 3   MATDYRVFDLNVTCAEGIQGVPSFVDFFHKWKVYAVVSVAGASPAGHRETPVAKYFGRNP 62
           M  DYRV DLN+T AEGI+GV SF +FF K KVYA VSV G +P+  +ETP+AKYFG+NP
Sbjct: 1   MLIDYRVLDLNITSAEGIKGVLSFTNFFFKSKVYAAVSVDG-TPSATQETPLAKYFGKNP 60

Query: 63  VWNFPMRFYFEESELRENALTALRIQLKRKRSFG-EDEDIAEALVPLKNLFHGNENVT-- 122
           VWN  MRF+ EES+L+ENALT L+I+LKR+R+FG +DEDIAEA +PL+NLF+GNENVT  
Sbjct: 61  VWNCSMRFHLEESKLQENALT-LKIKLKRRRTFGRDDEDIAEASIPLRNLFNGNENVTNE 120

Query: 123 NYTSSLLLSPSGGRRGFVYFLYYFSQIHRAT 151
           N +SS +LS SGG RG VYF Y FS+ + A+
Sbjct: 121 NCSSSFMLSKSGGLRGLVYFSYCFSRTYNAS 149

[Close]

BLAST of Spg003582 vs. NCBI nr
Match: XP_022150754.1 (protein SRC2 [Momordica charantia])

HSP 1 Score: 169.1 bits (427), Expect = 3.3e-38
Identity = 91/153 (59.48%), Postives = 112/153 (73.20%), Query Frame = 0


Query: 3   MATDYRVFDLNVTCAEGIQGVPSFVDFFHKWKVYAVVSVAGASPAGHRETPVAKYFGRNP 62
           M+TD+R F++N+TCAEG +GV SFV+FF K KVYA VSV G  PA  + TP+A YFG NP
Sbjct: 1   MSTDFREFNINITCAEGQKGVLSFVNFFQKTKVYAAVSVVGGEPA-VQFTPLANYFGDNP 60

Query: 63  VWNFPMRFYFEESELRENALTALRIQLKRKRSFGEDEDIAEALVPLKNLFHGNENVTNYT 122
           VWNFPMRF  EESEL+++ALT LRI+L+RKR FG DE IAEA V L NL + N++  N+ 
Sbjct: 61  VWNFPMRFCLEESELQQDALT-LRIELRRKRIFGGDEAIAEAFVRLGNLLNCNDSNLNFM 120

Query: 123 S-----SLLLSPSGGRRGFVYFLYYFSQIHRAT 151
                 S LLSP+GGRRG +YF Y F +  RAT
Sbjct: 121 DQICGFSWLLSPAGGRRGILYFSYCFGRTIRAT 151

[Close]

BLAST of Spg003582 vs. NCBI nr
Match: KAA0048022.1 (protein SRC2-like [Cucumis melo var. makuwa])

HSP 1 Score: 109.0 bits (271), Expect = 4.0e-20
Identity = 57/87 (65.52%), Postives = 70/87 (80.46%), Query Frame = 0


Query: 35  VYAVVSVAGASPAGHRETPVAKYFGRNPVWNFPMRFYFEESELRENALTALRIQLKRKRS 94
           VYA V V G  P+  +ETP+ K+FG NPVWNFPMRFY EES+L+ENALT L+I+LKRKR+
Sbjct: 277 VYAAVYVDGI-PSATQETPLEKHFGENPVWNFPMRFYLEESKLQENALT-LKIKLKRKRT 336

Query: 95  F-GEDEDIAEALVPLKNLFHGNENVTN 121
           F G  EDI EA + L+NLF+GN+NVTN
Sbjct: 337 FRGYGEDIGEASILLRNLFNGNQNVTN 361

[Close]

BLAST of Spg003582 vs. ExPASy Swiss-Prot
Match: O04133 (Protein SRC2 OS=Glycine max OX=3847 GN=SRC2 PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 6.1e-11
Identity = 51/147 (34.69%), Postives = 75/147 (51.02%), Query Frame = 0


Query: 3   MATDYRVFDLNVTCAEGIQGVPSFVDFFHKWKVYAVVSVAG--ASPAGHRETPVAKYFGR 62
           M  +YR  +LN+  A+ I+     V+ F K  VYA VS++G    P G   T V K  G 
Sbjct: 1   MTMEYRTLELNIISAKDIKN----VNLFSKMDVYAAVSLSGDPLHPQG-ATTHVHKDAGS 60

Query: 63  NPVWNFPMRFYFEESELRENALTALRIQLKRKRSFGEDEDIAEALVPLKNLFH--GNENV 122
           NP WN+P++F   ES  +EN L +L I+L   R+ G D  I    VPL+ L    G+++ 
Sbjct: 61  NPTWNYPVKFSVNESLAKENRL-SLEIKLISDRTLG-DTVIGTVHVPLRELLDNPGDDSS 120

Query: 123 TNYTSSLLLSPSGGRRGFVYFLYYFSQ 146
               S  ++  S   +G + F Y F +
Sbjct: 121 FRQVSYQVMKQSRKSKGSLNFSYKFGE 140

[Close]

BLAST of Spg003582 vs. ExPASy TrEMBL
Match: A0A6J1FDP3 (protein SRC2 OS=Cucurbita moschata OX=3662 GN=LOC111444443 PE=4 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 1.5e-49
Identity = 106/148 (71.62%), Postives = 122/148 (82.43%), Query Frame = 0


Query: 6   DYRVFDLNVTCAEGIQGVPSFVDFFHKWKVYAVVSVAGASPAGHRETPVAKYFGRNPVWN 65
           DYR F +NVTC EGI+GV SFV+FFHK KVYA VS+ G   A ++ETP+AKYFG NPVWN
Sbjct: 2   DYRAFKVNVTCVEGIEGVLSFVNFFHKSKVYAAVSITGDLLA-NQETPLAKYFGENPVWN 61

Query: 66  FPMRFYFEESELRENALTALRIQLKRKRSFGEDEDIAEALVPLKNLFHGNENV--TNYTS 125
           +PM+FY +ES+L+ENALT LRIQL+RKR FG DE IAEALVPLKNLF GN+NV   NY S
Sbjct: 62  WPMKFYLKESKLQENALT-LRIQLRRKRIFGGDEYIAEALVPLKNLFDGNKNVMDENYCS 121

Query: 126 SLLLSPSGGRRGFVYFLYYFSQIHRATA 152
           S LLSPSGGRRGFVYF YYFS+ + ATA
Sbjct: 122 SWLLSPSGGRRGFVYFSYYFSRTYAATA 147

[Close]

BLAST of Spg003582 vs. ExPASy TrEMBL
Match: A0A0A0LIU2 (C2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G030590 PE=4 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 3.7e-43
Identity = 97/151 (64.24%), Postives = 121/151 (80.13%), Query Frame = 0


Query: 3   MATDYRVFDLNVTCAEGIQGVPSFVDFFHKWKVYAVVSVAGASPAGHRETPVAKYFGRNP 62
           M  DYRV DLN+T AEGI+GV SF +FF K KVYA VSV G +P+  +ETP+AKYFG+NP
Sbjct: 1   MLIDYRVLDLNITSAEGIKGVLSFTNFFFKSKVYAAVSVDG-TPSATQETPLAKYFGKNP 60

Query: 63  VWNFPMRFYFEESELRENALTALRIQLKRKRSFG-EDEDIAEALVPLKNLFHGNENVT-- 122
           VWN  MRF+ EES+L+ENALT L+I+LKR+R+FG +DEDIAEA +PL+NLF+GNENVT  
Sbjct: 61  VWNCSMRFHLEESKLQENALT-LKIKLKRRRTFGRDDEDIAEASIPLRNLFNGNENVTNE 120

Query: 123 NYTSSLLLSPSGGRRGFVYFLYYFSQIHRAT 151
           N +SS +LS SGG RG VYF Y FS+ + A+
Sbjct: 121 NCSSSFMLSKSGGLRGLVYFSYCFSRTYNAS 149

[Close]

BLAST of Spg003582 vs. ExPASy TrEMBL
Match: A0A6J1DB07 (protein SRC2 OS=Momordica charantia OX=3673 GN=LOC111018814 PE=4 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 1.6e-38
Identity = 91/153 (59.48%), Postives = 112/153 (73.20%), Query Frame = 0


Query: 3   MATDYRVFDLNVTCAEGIQGVPSFVDFFHKWKVYAVVSVAGASPAGHRETPVAKYFGRNP 62
           M+TD+R F++N+TCAEG +GV SFV+FF K KVYA VSV G  PA  + TP+A YFG NP
Sbjct: 1   MSTDFREFNINITCAEGQKGVLSFVNFFQKTKVYAAVSVVGGEPA-VQFTPLANYFGDNP 60

Query: 63  VWNFPMRFYFEESELRENALTALRIQLKRKRSFGEDEDIAEALVPLKNLFHGNENVTNYT 122
           VWNFPMRF  EESEL+++ALT LRI+L+RKR FG DE IAEA V L NL + N++  N+ 
Sbjct: 61  VWNFPMRFCLEESELQQDALT-LRIELRRKRIFGGDEAIAEAFVRLGNLLNCNDSNLNFM 120

Query: 123 S-----SLLLSPSGGRRGFVYFLYYFSQIHRAT 151
                 S LLSP+GGRRG +YF Y F +  RAT
Sbjct: 121 DQICGFSWLLSPAGGRRGILYFSYCFGRTIRAT 151

[Close]

BLAST of Spg003582 vs. ExPASy TrEMBL
Match: A0A5A7TWY8 (Protein SRC2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G00900 PE=4 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 2.0e-20
Identity = 57/87 (65.52%), Postives = 70/87 (80.46%), Query Frame = 0


Query: 35  VYAVVSVAGASPAGHRETPVAKYFGRNPVWNFPMRFYFEESELRENALTALRIQLKRKRS 94
           VYA V V G  P+  +ETP+ K+FG NPVWNFPMRFY EES+L+ENALT L+I+LKRKR+
Sbjct: 277 VYAAVYVDGI-PSATQETPLEKHFGENPVWNFPMRFYLEESKLQENALT-LKIKLKRKRT 336

Query: 95  F-GEDEDIAEALVPLKNLFHGNENVTN 121
           F G  EDI EA + L+NLF+GN+NVTN
Sbjct: 337 FRGYGEDIGEASILLRNLFNGNQNVTN 361

[Close]

BLAST of Spg003582 vs. ExPASy TrEMBL
Match: A0A6P3ZA45 (protein SRC2 homolog OS=Ziziphus jujuba OX=326968 GN=LOC107411858 PE=4 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 1.4e-18
Identity = 60/146 (41.10%), Postives = 90/146 (61.64%), Query Frame = 0


Query: 6   DYRVFDLNVTCAEGIQGVPSFVDFFHKWKVYAVVSVAGASPAGHRETPVAKYFGRNPVWN 65
           +YR  D+NVTC EG Q V +   FF + K+YA VS+ G+S    ++TPV K+ G    W+
Sbjct: 2   EYRSLDINVTCLEGFQ-VKNL--FFKRPKIYATVSIVGSSRT-TQKTPVGKFAGSFQRWD 61

Query: 66  FPMRFYFEESELRENALTALRIQLKRKRSFGEDEDIAEALVPLKNLFHGNENVTNYTSSL 125
           +PMRFY EES L+EN L  L I+L+  R+   D+D+ E  VP+K LF  N++  +Y    
Sbjct: 62  YPMRFYIEESMLQENCL-MLMIRLRSSRTLCRDKDVGEVYVPIKQLFLHNDH-NSYFGYQ 121

Query: 126 LLSPSGGRRGFVYFLYYFSQIHRATA 152
           + + SG  +G ++F Y FS   R+++
Sbjct: 122 VHTASGKPKGRLHFSYKFSDRKRSSS 141

[Close]

BLAST of Spg003582 vs. TAIR 10
Match: AT3G05440.1 (C2 domain-containing protein )

HSP 1 Score: 49.7 bits (117), Expect = 2.7e-06
Identity = 29/101 (28.71%), Postives = 51/101 (50.50%), Query Frame = 0


Query: 7   YRVFDLNVTCAEGIQGVPSFVDFFHKWKVYAVVSVAGASPAGHRETPVAKYFGRNPVWNF 66
           Y+  ++NV  A+ +      ++   +  VY  VSV   S    + TP A +  +   WN+
Sbjct: 4   YQPVEINVLSAQDLNS----INLLFRPTVYVSVSVTRGS-RDKQVTPAAAWDKKFLRWNY 63

Query: 67  PMRFYFEESELRENALTALRIQLKRKRSFGEDEDIAEALVP 108
            M+FY E+ ++R N  +    Q+K KR FG D+ + +  +P
Sbjct: 64  RMKFYIEDDKVRRNE-SVFVFQIKCKRFFGSDQVVGKLFIP 98

The following BLAST results are available for this feature:

BLAST of Spg003582 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022938307.1	3.2e-49	71.62	protein SRC2 [Cucurbita moschata] >KAG7021095.1 Protein SRC2, partial [Cucurbita...	[more]
KAG6586252.1	3.2e-49	71.62	Protein SRC2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_031736682.1	7.6e-43	64.24	protein SRC2 homolog [Cucumis sativus] >KGN60949.1 hypothetical protein Csa_0211...	[more]
XP_022150754.1	3.3e-38	59.48	protein SRC2 [Momordica charantia]	[more]
KAA0048022.1	4.0e-20	65.52	protein SRC2-like [Cucumis melo var. makuwa]	[more]

BLAST of Spg003582 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04133	6.1e-11	34.69	Protein SRC2 OS=Glycine max OX=3847 GN=SRC2 PE=2 SV=1	[more]

BLAST of Spg003582 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FDP3	1.5e-49	71.62	protein SRC2 OS=Cucurbita moschata OX=3662 GN=LOC111444443 PE=4 SV=1	[more]
A0A0A0LIU2	3.7e-43	64.24	C2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G030590 PE=4 SV=...	[more]
A0A6J1DB07	1.6e-38	59.48	protein SRC2 OS=Momordica charantia OX=3673 GN=LOC111018814 PE=4 SV=1	[more]
A0A5A7TWY8	2.0e-20	65.52	Protein SRC2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G00...	[more]
A0A6P3ZA45	1.4e-18	41.10	protein SRC2 homolog OS=Ziziphus jujuba OX=326968 GN=LOC107411858 PE=4 SV=1	[more]

BLAST of Spg003582 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G05440.1	2.7e-06	28.71	C2 domain-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR035892	C2 domain superfamily	GENE3D	2.60.40.150	C2 domain	coord: 8..135 e-value: 9.1E-10 score: 40.5
IPR035892	C2 domain superfamily	SUPERFAMILY	49562	C2 domain (Calcium/lipid-binding domain, CaLB)	coord: 12..143
IPR000008	C2 domain	PFAM	PF00168	C2	coord: 10..119 e-value: 7.7E-6 score: 26.2
IPR000008	C2 domain	PROSITE	PS50004	C2	coord: 1..121 score: 11.259693
None	No IPR available	PANTHER	PTHR32246	INGRESSION PROTEIN FIC1	coord: 8..147
None	No IPR available	PANTHER	PTHR32246:SF101	OS01G0934100 PROTEIN	coord: 8..147

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg003582.1	Spg003582.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response