Spg003525 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg003525
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Early nodulin 93 protein
Location	scaffold4: 47809334 .. 47811776 (+)
RNA-Seq Expression	Spg003525
Synteny	Spg003525

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAATACAGACATCTATTCGAATGAGTATATCATGTAATCATTAAAGAATATCATCGATGTATCACATAAGTTTCAAACAAACAAGTTAACATGAACATAACTCAACTGACATAAAGTTATATTAATGACCAAGATGTCTGTGGTTCGAATTTCCCCCCTCACAAGTTAAAAAAAATTCCAAACAAACAAAATTAACCATACCTGTGTCAAAGTGAGCATAACATGGTCTAAAATAAAAAAAGAAAAAATCTCACCAAACAAAAACCTAACAGATGAAAATAAGAGGAGGGGTATAAACGGAATTTCGTCATAAACCAGGGACATTTTGGGAAGAAATGCCGAGATAATCAACCAACTGCAACAGAAACCAGAGGAAAAGAGAAGAAAACAAGAGTCTCTGAGAGACGCTGGAATTTTGGAAAACCATCAAACTCTCTTCCACCAATGACACCATCTGAGCTTCAAGCTCTCAAAATCACTTCCTTTCATTCACTCATCTTAAATGTTTATAAATACCCAACAAACATTTCAGTTTCACTTCAACCCAAATCATCCCCTGCAGCGTTTTTGATTTCTTGTTCTTACGACAATGGCGAAGAATGTAGCTCAGGCCTTTTTCGAGAGGAAAAATCTGGCCTCCCATGACCCAAAAGTGGCAATGGCCAAGCGCTGTGCCAAAGGTAATAAAATGCTTTTTGATCTTTTTCTTTTTTTCTGGGATTTCTGATGAGGAACAAAGAAAAAGTAATGCGTATGCAAGGTTTTTAGTTGCTGTGATTTGATGATACCACAGTGTGATGTATTTGATGAGTTGGGTCGTTGAAAGTAGTGACTGATGGATCATGATAATGGGGTTAAGATTTGGTTTTTTGATAATGCCTTTTAGCTGTTTGATGAAAGTCCTCAAAGAGGGTTTTGTTCTTTTAGCTTCAAGAATGGATTGAAAGTAAAAGCTTGGGGGGCCATGTTCAACTTCTCCCACTCCAACTTCCCCACCAACTCTTCTTTTTCAAGTTAATGATTTTAAGTAAAAGCTAAAGAGAGATGGGATGTGATCCATGCGCATGTTGATTGGATATATGCTGTGTTTTTGACTGATTTGATTGTTTTGATCGATTGCCTGGTGGACCATGATCCACTTCAGATTTGGTGCCATGAAAATAAGTGAATTCTGATGTTGTGTTTGTGTTTCTAATAATTCTATTCACAAATTTAGCTTTTATCATCAGTATCTGAAGCTAATGGGTTATGTTTCTTCTTTTTTGATGGACAGAGGGTGTGATAGCAGGAGCAAAAGCAGCTGCCATTGCAAGTATTGCCACTGCCATTCCCACTGTAAGTTCCCAACTTCATAAAGCACACACTGATTCTTATCTTTCCTTTTTGGAGAATAATTAGTGTCACTCATCAGCCTCTGCTTTTATTCGAAAAATATGACCAAATTAGCTGAAGAACTAAATCCTCAATTGATGTAGATTGCCTTAAGTGGCACAACTTAATCCTCAACTGATTTGTATTAGATAAGAATGTTTCTTTAAGTTGGAGGGTTGAGGCACCACAAGTATGTAGTGTAGCCCTATTCGAGTTTAGGTCTAAGGGCAGAGCGAAGAAGAAAGGAGGGAGGCAATTGTCTATTCATCTTATACTTAAAAAATTGTGTAAAGAAGTTGAAATTTTGAACTTTACTTTCTAATTTGCACGCTAAGAATGTAAAATTTTGTCTCTCCCTATGAAAATGTCTCGATCTTCCCCTGAACGGGGTCGAGGCACCAGGCAGCCGCTCTTTCACCACTCAAACACGGTGTAGAGCTAGCTGTTGCTGCCACGTCCTTCAACCCGAATTCTGTCGAAATATTGTAGAAACTAGAGTCGATCGAGCCAATCCATGACTAGAATTGTTATTATTTTGCAGTTTGCTAGTGTGAGGATGCTGCCATGGGCAAAAGCTCATCTGAATTACACAGCTCAAGCTCTAATAATCTCCACAGGTTCAACTTTTTTCTTTTCTCTTGCAGCCAAGTAAATGTATGGTTTCACTTTCATTTTCTTTCCTTTACTAAGGTAAAAAAGGCTTGGTTTTTCAATGGCAGTGGCTGGAGCAGCATACTTCATAGTGGCTGACAAGACAGTTCTGGCAACAGCAAGAAGAAACTCCTTCAAGCAAAATCCCAACACTGAAGCATATGAATTCAATTGAGTTCTCAACCTTAAAATCTGAGGCTACCCATTGAAGAAGAAGAAGCTACCCTCATCTGGATTTGAGGGCAGGCATTGAAGAAGCTAGCCATCTCAAAAAGTTGCTAGCTTTTGCTCTTTCGGTTTTACTTGAATCTTGTGCTATGAAAATCTATGTAAAATCCATTTACTATTTGAATAAATAAAAATGGGTTTTCGGACCTTTGTCTCGTGAAATTAGTCGAACATTTGCTCTTATTAGATTAC

mRNA sequence

ATGGCGAAGAATGTAGCTCAGGCCTTTTTCGAGAGGAAAAATCTGGCCTCCCATGACCCAAAAGTGGCAATGGCCAAGCGCTGTGCCAAAGAGGGTGTGATAGCAGGAGCAAAAGCAGCTGCCATTGCAAGTATTGCCACTGCCATTCCCACTTTTGCTAGTGTGAGGATGCTGCCATGGGCAAAAGCTCATCTGAATTACACAGCTCAAGCTCTAATAATCTCCACAGTGGCTGGAGCAGCATACTTCATAGTGGCTGACAAGACAGTTCTGGCAACAGCAAGAAGAAACTCCTTCAAGCAAAATCCCAACACTGAAGCATATGAATTCAATTGA

Coding sequence (CDS)

ATGGCGAAGAATGTAGCTCAGGCCTTTTTCGAGAGGAAAAATCTGGCCTCCCATGACCCAAAAGTGGCAATGGCCAAGCGCTGTGCCAAAGAGGGTGTGATAGCAGGAGCAAAAGCAGCTGCCATTGCAAGTATTGCCACTGCCATTCCCACTTTTGCTAGTGTGAGGATGCTGCCATGGGCAAAAGCTCATCTGAATTACACAGCTCAAGCTCTAATAATCTCCACAGTGGCTGGAGCAGCATACTTCATAGTGGCTGACAAGACAGTTCTGGCAACAGCAAGAAGAAACTCCTTCAAGCAAAATCCCAACACTGAAGCATATGAATTCAATTGA

Protein sequence

MAKNVAQAFFERKNLASHDPKVAMAKRCAKEGVIAGAKAAAIASIATAIPTFASVRMLPWAKAHLNYTAQALIISTVAGAAYFIVADKTVLATARRNSFKQNPNTEAYEFN

Homology

[Close]

BLAST of Spg003525 vs. NCBI nr
Match: XP_004139321.1 (early nodulin-93 [Cucumis sativus] >KGN60663.1 hypothetical protein Csa_019393 [Cucumis sativus])

HSP 1 Score: 199.9 bits (507), Expect = 1.1e-47
Identity = 106/111 (95.50%), Postives = 109/111 (98.20%), Query Frame = 0


Query: 1   MAKNVAQAFFERKNLASHDPKVAMAKRCAKEGVIAGAKAAAIASIATAIPTFASVRMLPW 60
           MAKN+AQAF ERKN+ASHDPKVAMAKRCAKEGVIAGAKAAAIASIATAIPTFASVRMLPW
Sbjct: 1   MAKNLAQAFLERKNIASHDPKVAMAKRCAKEGVIAGAKAAAIASIATAIPTFASVRMLPW 60

Query: 61  AKAHLNYTAQALIISTVAGAAYFIVADKTVLATARRNSFKQNPNTEAYEFN 112
           AKAHLN+TAQALIISTVAGAAYFIVADKTVLATARRNSFKQ PNTEAYEFN
Sbjct: 61  AKAHLNHTAQALIISTVAGAAYFIVADKTVLATARRNSFKQKPNTEAYEFN 111

[Close]

BLAST of Spg003525 vs. NCBI nr
Match: KAA0061591.1 (early nodulin-93-like [Cucumis melo var. makuwa] >TYJ97800.1 early nodulin-93-like [Cucumis melo var. makuwa])

HSP 1 Score: 199.5 bits (506), Expect = 1.5e-47
Identity = 106/111 (95.50%), Postives = 109/111 (98.20%), Query Frame = 0


Query: 1   MAKNVAQAFFERKNLASHDPKVAMAKRCAKEGVIAGAKAAAIASIATAIPTFASVRMLPW 60
           MAKN+AQAF ERKNLASHDPKVAMAKRCA+EGVIAGAKAAAIASIATAIPTFASVRMLPW
Sbjct: 1   MAKNLAQAFLERKNLASHDPKVAMAKRCAREGVIAGAKAAAIASIATAIPTFASVRMLPW 60

Query: 61  AKAHLNYTAQALIISTVAGAAYFIVADKTVLATARRNSFKQNPNTEAYEFN 112
           AKAHLN+TAQALIISTVAGAAYFIVADKTVLATARRNSFKQ PNTEAYEFN
Sbjct: 61  AKAHLNHTAQALIISTVAGAAYFIVADKTVLATARRNSFKQKPNTEAYEFN 111

[Close]

BLAST of Spg003525 vs. NCBI nr
Match: XP_008457774.1 (PREDICTED: early nodulin-93-like [Cucumis melo])

HSP 1 Score: 199.5 bits (506), Expect = 1.5e-47
Identity = 106/111 (95.50%), Postives = 109/111 (98.20%), Query Frame = 0


Query: 1   MAKNVAQAFFERKNLASHDPKVAMAKRCAKEGVIAGAKAAAIASIATAIPTFASVRMLPW 60
           MAKN+AQAF ERKNLASHDPKVAMAKRCA+EGVIAGAKAAAIASIATAIPTFASVRMLPW
Sbjct: 39  MAKNLAQAFLERKNLASHDPKVAMAKRCAREGVIAGAKAAAIASIATAIPTFASVRMLPW 98

Query: 61  AKAHLNYTAQALIISTVAGAAYFIVADKTVLATARRNSFKQNPNTEAYEFN 112
           AKAHLN+TAQALIISTVAGAAYFIVADKTVLATARRNSFKQ PNTEAYEFN
Sbjct: 99  AKAHLNHTAQALIISTVAGAAYFIVADKTVLATARRNSFKQKPNTEAYEFN 149

[Close]

BLAST of Spg003525 vs. NCBI nr
Match: XP_022937499.1 (early nodulin-93-like [Cucurbita moschata])

HSP 1 Score: 198.0 bits (502), Expect = 4.4e-47
Identity = 105/111 (94.59%), Postives = 108/111 (97.30%), Query Frame = 0


Query: 1   MAKNVAQAFFERKNLASHDPKVAMAKRCAKEGVIAGAKAAAIASIATAIPTFASVRMLPW 60
           MAKN+AQAF ERKNLASHDPKVAMAKRCAKEGVIAGAKAAAIAS+ATAIPT ASVRMLPW
Sbjct: 1   MAKNLAQAFLERKNLASHDPKVAMAKRCAKEGVIAGAKAAAIASVATAIPTLASVRMLPW 60

Query: 61  AKAHLNYTAQALIISTVAGAAYFIVADKTVLATARRNSFKQNPNTEAYEFN 112
           AKAHLN+TAQALIISTVAGAAYFIVADKTVLATARRNSFKQNPNTEAYE N
Sbjct: 61  AKAHLNHTAQALIISTVAGAAYFIVADKTVLATARRNSFKQNPNTEAYELN 111

[Close]

BLAST of Spg003525 vs. NCBI nr
Match: XP_023537923.1 (early nodulin-93-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 197.2 bits (500), Expect = 7.4e-47
Identity = 104/111 (93.69%), Postives = 108/111 (97.30%), Query Frame = 0


Query: 1   MAKNVAQAFFERKNLASHDPKVAMAKRCAKEGVIAGAKAAAIASIATAIPTFASVRMLPW 60
           MAKN++QAFFERKNL SHDPKVAMAKRCAKEGVIAGAKAAAIAS+ATAIPT ASVRMLPW
Sbjct: 1   MAKNLSQAFFERKNLPSHDPKVAMAKRCAKEGVIAGAKAAAIASVATAIPTLASVRMLPW 60

Query: 61  AKAHLNYTAQALIISTVAGAAYFIVADKTVLATARRNSFKQNPNTEAYEFN 112
           AKAHLN+TAQALIISTVAGAAYFIVADKTVLATARRNSFKQNPNTEAYE N
Sbjct: 61  AKAHLNHTAQALIISTVAGAAYFIVADKTVLATARRNSFKQNPNTEAYELN 111

[Close]

BLAST of Spg003525 vs. ExPASy Swiss-Prot
Match: Q02921 (Early nodulin-93 OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 1.7e-30
Identity = 72/97 (74.23%), Postives = 84/97 (86.60%), Query Frame = 0


Query: 11  ERKNLASHDPKVAMAKRCAKEGVIAGAKAAAIASIATAIPTFASVRMLPWAKAHLNYTAQ 70
           ER +LAS D K+A AKRC+ EGV+AGAKAA +AS+A+AIPT ASVRMLPWA+A+LN+TAQ
Sbjct: 9   ERPSLASLDQKLAFAKRCSHEGVLAGAKAAVVASVASAIPTLASVRMLPWARANLNHTAQ 68

Query: 71  ALIISTVAGAAYFIVADKTVLATARRNSFKQNPNTEA 108
           ALIIST   AAYFIVADKTVLATAR+NSF Q  N+EA
Sbjct: 69  ALIISTATAAAYFIVADKTVLATARKNSFNQPSNSEA 105

[Close]

BLAST of Spg003525 vs. ExPASy TrEMBL
Match: A0A0A0LFY1 (Early nodulin 93 protein OS=Cucumis sativus OX=3659 GN=Csa_2G006090 PE=4 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 5.6e-48
Identity = 106/111 (95.50%), Postives = 109/111 (98.20%), Query Frame = 0


Query: 1   MAKNVAQAFFERKNLASHDPKVAMAKRCAKEGVIAGAKAAAIASIATAIPTFASVRMLPW 60
           MAKN+AQAF ERKN+ASHDPKVAMAKRCAKEGVIAGAKAAAIASIATAIPTFASVRMLPW
Sbjct: 1   MAKNLAQAFLERKNIASHDPKVAMAKRCAKEGVIAGAKAAAIASIATAIPTFASVRMLPW 60

Query: 61  AKAHLNYTAQALIISTVAGAAYFIVADKTVLATARRNSFKQNPNTEAYEFN 112
           AKAHLN+TAQALIISTVAGAAYFIVADKTVLATARRNSFKQ PNTEAYEFN
Sbjct: 61  AKAHLNHTAQALIISTVAGAAYFIVADKTVLATARRNSFKQKPNTEAYEFN 111

[Close]

BLAST of Spg003525 vs. ExPASy TrEMBL
Match: A0A5A7V781 (Early nodulin-93-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G00100 PE=4 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 7.3e-48
Identity = 106/111 (95.50%), Postives = 109/111 (98.20%), Query Frame = 0


Query: 1   MAKNVAQAFFERKNLASHDPKVAMAKRCAKEGVIAGAKAAAIASIATAIPTFASVRMLPW 60
           MAKN+AQAF ERKNLASHDPKVAMAKRCA+EGVIAGAKAAAIASIATAIPTFASVRMLPW
Sbjct: 1   MAKNLAQAFLERKNLASHDPKVAMAKRCAREGVIAGAKAAAIASIATAIPTFASVRMLPW 60

Query: 61  AKAHLNYTAQALIISTVAGAAYFIVADKTVLATARRNSFKQNPNTEAYEFN 112
           AKAHLN+TAQALIISTVAGAAYFIVADKTVLATARRNSFKQ PNTEAYEFN
Sbjct: 61  AKAHLNHTAQALIISTVAGAAYFIVADKTVLATARRNSFKQKPNTEAYEFN 111

[Close]

BLAST of Spg003525 vs. ExPASy TrEMBL
Match: A0A1S3C6F4 (early nodulin-93-like OS=Cucumis melo OX=3656 GN=LOC103497382 PE=4 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 7.3e-48
Identity = 106/111 (95.50%), Postives = 109/111 (98.20%), Query Frame = 0


Query: 1   MAKNVAQAFFERKNLASHDPKVAMAKRCAKEGVIAGAKAAAIASIATAIPTFASVRMLPW 60
           MAKN+AQAF ERKNLASHDPKVAMAKRCA+EGVIAGAKAAAIASIATAIPTFASVRMLPW
Sbjct: 39  MAKNLAQAFLERKNLASHDPKVAMAKRCAREGVIAGAKAAAIASIATAIPTFASVRMLPW 98

Query: 61  AKAHLNYTAQALIISTVAGAAYFIVADKTVLATARRNSFKQNPNTEAYEFN 112
           AKAHLN+TAQALIISTVAGAAYFIVADKTVLATARRNSFKQ PNTEAYEFN
Sbjct: 99  AKAHLNHTAQALIISTVAGAAYFIVADKTVLATARRNSFKQKPNTEAYEFN 149

[Close]

BLAST of Spg003525 vs. ExPASy TrEMBL
Match: A0A6J1FBC4 (early nodulin-93-like OS=Cucurbita moschata OX=3662 GN=LOC111443891 PE=4 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 2.1e-47
Identity = 105/111 (94.59%), Postives = 108/111 (97.30%), Query Frame = 0


Query: 1   MAKNVAQAFFERKNLASHDPKVAMAKRCAKEGVIAGAKAAAIASIATAIPTFASVRMLPW 60
           MAKN+AQAF ERKNLASHDPKVAMAKRCAKEGVIAGAKAAAIAS+ATAIPT ASVRMLPW
Sbjct: 1   MAKNLAQAFLERKNLASHDPKVAMAKRCAKEGVIAGAKAAAIASVATAIPTLASVRMLPW 60

Query: 61  AKAHLNYTAQALIISTVAGAAYFIVADKTVLATARRNSFKQNPNTEAYEFN 112
           AKAHLN+TAQALIISTVAGAAYFIVADKTVLATARRNSFKQNPNTEAYE N
Sbjct: 61  AKAHLNHTAQALIISTVAGAAYFIVADKTVLATARRNSFKQNPNTEAYELN 111

[Close]

BLAST of Spg003525 vs. ExPASy TrEMBL
Match: A0A6J1HQF2 (early nodulin-93-like OS=Cucurbita maxima OX=3661 GN=LOC111465732 PE=4 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 4.7e-47
Identity = 104/111 (93.69%), Postives = 108/111 (97.30%), Query Frame = 0


Query: 1   MAKNVAQAFFERKNLASHDPKVAMAKRCAKEGVIAGAKAAAIASIATAIPTFASVRMLPW 60
           MAKN++QAF ERKNLASHDPKVAMAKRCAKEGVIAGAKAAAIAS+ATAIPT ASVRMLPW
Sbjct: 1   MAKNLSQAFLERKNLASHDPKVAMAKRCAKEGVIAGAKAAAIASVATAIPTLASVRMLPW 60

Query: 61  AKAHLNYTAQALIISTVAGAAYFIVADKTVLATARRNSFKQNPNTEAYEFN 112
           AKAHLN+TAQALIISTVAGAAYFIVADKTVLATARRNSFKQNPNTEAYE N
Sbjct: 61  AKAHLNHTAQALIISTVAGAAYFIVADKTVLATARRNSFKQNPNTEAYELN 111

[Close]

BLAST of Spg003525 vs. TAIR 10
Match: AT5G25940.1 (early nodulin-related )

HSP 1 Score: 92.4 bits (228), Expect = 2.4e-19
Identity = 51/81 (62.96%), Postives = 65/81 (80.25%), Query Frame = 0


Query: 21  KVAMAKRCAKEGVIAGAKAAAIASIATAIPTFASVRMLPWAKAHLNYTAQALIISTVAGA 80
           K+  A+   + G IAGAKAAA+A++A+AIPT A+VRM PWAKA+LNYTAQALIIS+ + A
Sbjct: 26  KIHRAETSKQAGAIAGAKAAAVAAVASAIPTVAAVRMFPWAKANLNYTAQALIISSASIA 85

Query: 81  AYFIVADKTVLATARRNSFKQ 102
           A+FI ADKT+L  ARRN+  Q
Sbjct: 86  AFFITADKTILQGARRNTEAQ 106

The following BLAST results are available for this feature:

BLAST of Spg003525 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004139321.1	1.1e-47	95.50	early nodulin-93 [Cucumis sativus] >KGN60663.1 hypothetical protein Csa_019393 [...	[more]
KAA0061591.1	1.5e-47	95.50	early nodulin-93-like [Cucumis melo var. makuwa] >TYJ97800.1 early nodulin-93-li...	[more]
XP_008457774.1	1.5e-47	95.50	PREDICTED: early nodulin-93-like [Cucumis melo]	[more]
XP_022937499.1	4.4e-47	94.59	early nodulin-93-like [Cucurbita moschata]	[more]
XP_023537923.1	7.4e-47	93.69	early nodulin-93-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Spg003525 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q02921	1.7e-30	74.23	Early nodulin-93 OS=Glycine max OX=3847 PE=2 SV=1	[more]

BLAST of Spg003525 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LFY1	5.6e-48	95.50	Early nodulin 93 protein OS=Cucumis sativus OX=3659 GN=Csa_2G006090 PE=4 SV=1	[more]
A0A5A7V781	7.3e-48	95.50	Early nodulin-93-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold28...	[more]
A0A1S3C6F4	7.3e-48	95.50	early nodulin-93-like OS=Cucumis melo OX=3656 GN=LOC103497382 PE=4 SV=1	[more]
A0A6J1FBC4	2.1e-47	94.59	early nodulin-93-like OS=Cucurbita moschata OX=3662 GN=LOC111443891 PE=4 SV=1	[more]
A0A6J1HQF2	4.7e-47	93.69	early nodulin-93-like OS=Cucurbita maxima OX=3661 GN=LOC111465732 PE=4 SV=1	[more]

BLAST of Spg003525 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G25940.1	2.4e-19	62.96	early nodulin-related	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005050	Early nodulin 93 ENOD93 protein	PFAM	PF03386	ENOD93	coord: 21..98 e-value: 2.8E-39 score: 132.8
IPR005050	Early nodulin 93 ENOD93 protein	PANTHER	PTHR33605	EARLY NODULIN-93	coord: 13..105

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg003525.1	Spg003525.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane