Spg003477 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg003477
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Mediator of RNA polymerase II transcription subunit 20
Location	scaffold4: 49119881 .. 49123318 (-)
RNA-Seq Expression	Spg003477
Synteny	Spg003477

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAACTTGGCAAGTACTCAAGTTTGGGCTGGCAGGCGTCTTTATTGTTTCCATTGGGCTTTCCGCAATAGTTGCAACTTGTTCCTTACCTGCTCCGAGTCCGAACTGACTGACCTCCGTCGTCTCGCTGCGACTCCCTACGGCTGAGGGTATGCCGCTAAAATGGTAAAACCCCAGGAACCCCGACCGAAAAAATTATCGTCCCTTTTCGTGTGGATTTGATTCTTGATCTAGTTTCATCGTTTTCTTGTGCTTAGGGTTTTGCATTGGCAACCGAATGCCGGCAGCACGGTTAACAGTCAGATCCTCAACGAAGTTTCGCAATGTGTTGAGAGTATCCATGGCGTTAAAGGGGGCAAGTGGAAAGCTAATCTCACTTTCTACAAACCCATCTTACGAGGTAATCTAACTCTTTTCCTAGAGGCGTTTGTCGGGATTTGATTACCTAAATGAATCTTAAATCTGCCAACAGATCAGAACATACCGGCGGCTGAGTTCCCGCGCGAATTTCTCGGAATTTCGCTGCCGGAGCAGCCAAACAAGTATTATCTTATAATTCGTGGCCAACGGATCGTCTTAGAAGCCGATTTTTCGATTCTGACTATAATGGAGAAGCTGCAGTCTTACAAATTGCGCGTTGCTCTCAATTTCGAGGTTGACTTCACCGCCCATCTTTTCTAGTTCTGATGAACTCTGCATTTTCCCTTGTCCAGAAATTCAAATTTGAAGTTGATTTTTGTAGCTTTCTGGTCTCTACACTTATGCCCTTTTGTTTCTAGTCGTGTGAGATAGATAAACCTTTGGTGACTGACAGTTCGCCTTGTTGTTTTTTAGGGGTTCCAATACCAATTAGGGGATTTCCAACTGAGAGTGGGAAAAGTTGTTCCCAACCATTCGGAGAACTTGAGAGGAATAGTTATGGAGGTCAGGCTCATTTCATCATTTCATGTCAACTCTGTTCTTGTGTTATTGCATGCTCTTCTGGTTGGTTTTTTTTATGTCCGTTTCTTTATCTGAGCGAGCCGTTTATTTTCCATGGACCTTTCAAATCCATGTTTGGCCTCTGTTGTTCTCATCTTGAAAACAATGAAGTAGAAGGAACAGGCTAATCATCACTAGAAGTATCATTTTCTTTGAAGAACTTATCAGTAAGCTTCGGAGTCGGGATCTTATGTTGAGCAGTGGTTAGATCTGTTTAGGATATCTTTGCAAAAGCCTATGTAGGAATAGTTATGGAAGTCCTTATTGTGATCTTCACATTTGGTTATGTTTCATATTCATCGTTGCACATGAATCAAAGTGTTTTGAGAAAATTCCTCTCCAAGAGGTGATTTTACTCGGTTTGGAAAGTGCCTTTCAGTTCACTTGAATGCTTGATGACAGACAGTCTTCTGTCTTTGCTTGGGCTCGATTCCCTTATGGTACTCATCCTGAAACAAATAAAGCAAAGAAAAACTCTGACTTCGAAAATCTATTCTTATTGTCTTTTTTTCTTTCTCTCCAAATTCTTCTTCAAAAACACATTACCATGTTCCCTATTGCATTGTCAAGTCCAAGTCCAAGTAGTATATACTATCAAGTCTGATGAAACCTCCCTGAGATGGATTGCTTGCATACTGAAGTTGGAAACCTGAGGCTTGTCTAGCAAATCAATGATTCAATTCTGTAATTTTTTTAAAATCTATTCTGATTGAATGTCATTTTGGAAGTGTTGTTAAATTTGGGAAGTAAGACCTTATATTCAAGTGGGATTTAATTAGAAACTAGAATCTCTCTGTCAAATGTTCAAATGTTATATTTTTGCGCTGTAAATATGAAGATTGCTCTTTTTTGCTGATCCAATGAAACATTTCAAACACTCGAGACTATATACAACTCCTGTTGTAGATATGCAATAATGTATTAAATCCTGTATATTGCAGATTGAGTATCTCCCAATTTCTTCGATGGAGAAATCAAGACAGGTGATGGAGGAGTTCTTCGAGGTATGGCAAGAAGCCATGTCAAAAAGTTCATTACCAGGTCGTTTCAAGCACATAGAACCAAATTTTGCAGAATATGGACTATCTGATTATTACACTTCTCAACACACAGCGGTTCAATATGCCAATGTGATGGCTCAGCTAATCGCTACTGTGCAAGCAGTTCAAGCAGTGAGAAATTAGGATAAAAATTGTTTGTGTTATCAGCTTTGAGATTTGAACTTTATGTTAGCTGTTGTCCTTCTTTTTCAAATGATGGAAGACTAGATTTGACTGAAATTGTTCGTGCTTGAAAATGTTCAACATAGAATGGACGCCAAGGCGCTGTGCATCAAACAGAGCAGTAACTAAAAAAATCGAAATGCTAAAATTTCAAAAGTGCGTCTGCCATCTAACATTACGGTGGACGCTTTTATTCAATGACAACAACCACGACATTGTTCATCAGAAATTCAAAGCGACAATGATAGGAAGGCTTAGAGACTCGCCGTTTGCGGCGGCGGAACTATGTAATCCACCACGATCACACTCTCCAAATGCTCCCTGACCTTCTCTTTCTGCAATTCCGCCATTTCTTGGTCTGAAGCCGCCGCCTCCAACTCATTCGCCCCCGGGAAAATTGCAATCGATGCGATTGAATACCCCTTGGCTCTGGCTGGGGAAAAGTTCTCGCCCACCGTCAGCTGGACATTCGGCCCCAATTTCTCCCTCAGCCCTCCAATGACCTCCAAGATCTCGCCTTTAACTTCGTCGCCGAGATTGTCCTTGAGTTTGAGGAAGGTGACTCTAACGGCGGATCCAGGAGGTGCGGTCACCGGGCCTTGCAACGCATCGGCGACCCAGTCGACGGCCATGATGTCGTCGCAGATGGGAAGAACAAATTGCTTGACGACGTTGACATGGCTTGGGTGGGCGGAGTAGGCGGAGAGATCCTCCTTGGAGGCGTAGCGGCTGTGAAGGAGGTGAGTGAAATTGGAGGCGGAGGAGCGGTTGCGGAGAATGGGACCGGCGGAGAGATGGAGGACTTGGTCGAGGGAGACGAGGCTATTGAGTCCGGTGAGCATGTGGGTCGAATCGACGCCGTCTTTAACCTTGAAGAGGACGACGTGTTCGATTGGGTGAGTGTGAGTGGACATGTTGACGGAGAAGGGTTTGAAGGAGGGACGAGGGTTTGGTCGGAGGCGGAGGTGTCTGTAAGGGAGCGTGGAGAAAGTCCGGAGAGCAAAGGCGAGCATTTTAACGCCAACGCAACGCCAAATCAGAATCAGAGAATTTGGGCGACACTGACAGGGGCGTGCTGCGTAGTGCGTTCACCGCCAACCATACACAAAAGAACTCCTAAAATATCAATCACGCGAATCATGTCCGTTCATTTTATGTTTGATTCTTTAGTTCTTAATGCGTGGGGAAAGTATTCTAATACCTCTAATCTAATATATCAAAACACAGAGATG

mRNA sequence

ATGGGAACTTGGCAAGTACTCAAGTTTGGGCTGGCAGGCGTCTTTATTGTTTCCATTGGGCTTTCCGCAATAGTTGCAACTTGTTCCTTACCTGCTCCGAGGTTTTGCATTGGCAACCGAATGCCGGCAGCACGGTTAACAGTCAGATCCTCAACGAAGTTTCGCAATGTGTTGAGAGTATCCATGGCGTTAAAGGGGGCAAGTGGAAAGCTAATCTCACTTTCTACAAACCCATCTTACGAGAACATACCGGCGGCTGAGTTCCCGCGCGAATTTCTCGGAATTTCGCTGCCGGAGCAGCCAAACAAGTATTATCTTATAATTCGTGGCCAACGGATCGTCTTAGAAGCCGATTTTTCGATTCTGACTATAATGGAGAAGCTGCAGTCTTACAAATTGCGCGTTGCTCTCAATTTCGAGGGGTTCCAATACCAATTAGGGGATTTCCAACTGAGAGTGGGAAAAGTTGTTCCCAACCATTCGGAGAACTTGAGAGGAATAGTTATGGAGGTCAGGCTCATTTCATCATTTCATGTCAACTCTGTTCTTGTGTTATTGCATGCTCTTCTGGTTGACAGTCTTCTGTCTTTGCTTGGGCTCGATTCCCTTATGATTGAGTATCTCCCAATTTCTTCGATGGAGAAATCAAGACAGGTGATGGAGGAGTTCTTCGAGGTATGGCAAGAAGCCATGTCAAAAAGTTCATTACCAGGTCGTTTCAAGCACATAGAACCAAATTTTGCAGAATATGGACTATCTGATTATTACACTTCTCAACACACAGCGGTTCAATATGCCAATGTGATGGCTCAGCTAATCGCTACTGTGCAAGCAGTTCAAGCAGTGAGAAATTAG

Coding sequence (CDS)

ATGGGAACTTGGCAAGTACTCAAGTTTGGGCTGGCAGGCGTCTTTATTGTTTCCATTGGGCTTTCCGCAATAGTTGCAACTTGTTCCTTACCTGCTCCGAGGTTTTGCATTGGCAACCGAATGCCGGCAGCACGGTTAACAGTCAGATCCTCAACGAAGTTTCGCAATGTGTTGAGAGTATCCATGGCGTTAAAGGGGGCAAGTGGAAAGCTAATCTCACTTTCTACAAACCCATCTTACGAGAACATACCGGCGGCTGAGTTCCCGCGCGAATTTCTCGGAATTTCGCTGCCGGAGCAGCCAAACAAGTATTATCTTATAATTCGTGGCCAACGGATCGTCTTAGAAGCCGATTTTTCGATTCTGACTATAATGGAGAAGCTGCAGTCTTACAAATTGCGCGTTGCTCTCAATTTCGAGGGGTTCCAATACCAATTAGGGGATTTCCAACTGAGAGTGGGAAAAGTTGTTCCCAACCATTCGGAGAACTTGAGAGGAATAGTTATGGAGGTCAGGCTCATTTCATCATTTCATGTCAACTCTGTTCTTGTGTTATTGCATGCTCTTCTGGTTGACAGTCTTCTGTCTTTGCTTGGGCTCGATTCCCTTATGATTGAGTATCTCCCAATTTCTTCGATGGAGAAATCAAGACAGGTGATGGAGGAGTTCTTCGAGGTATGGCAAGAAGCCATGTCAAAAAGTTCATTACCAGGTCGTTTCAAGCACATAGAACCAAATTTTGCAGAATATGGACTATCTGATTATTACACTTCTCAACACACAGCGGTTCAATATGCCAATGTGATGGCTCAGCTAATCGCTACTGTGCAAGCAGTTCAAGCAGTGAGAAATTAG

Protein sequence

MGTWQVLKFGLAGVFIVSIGLSAIVATCSLPAPRFCIGNRMPAARLTVRSSTKFRNVLRVSMALKGASGKLISLSTNPSYENIPAAEFPREFLGISLPEQPNKYYLIIRGQRIVLEADFSILTIMEKLQSYKLRVALNFEGFQYQLGDFQLRVGKVVPNHSENLRGIVMEVRLISSFHVNSVLVLLHALLVDSLLSLLGLDSLMIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFKHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQAVRN

Homology

[Close]

BLAST of Spg003477 vs. NCBI nr
Match: XP_038889134.1 (mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Benincasa hispida])

HSP 1 Score: 298.9 bits (764), Expect = 4.6e-77
Identity = 159/204 (77.94%), Postives = 166/204 (81.37%), Query Frame = 0


Query: 81  ENIPAAEFPREFLGISLPEQPNKYYLIIRGQRIVLEADFSILTIMEKLQSYKLRVALNFE 140
           +N+PAAEFPR+FLGISLPEQPNKYYLIIRGQRIVLEADF+I  IMEKLQSYKLRVALNFE
Sbjct: 54  QNLPAAEFPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFE 113

Query: 141 GFQYQLGDFQLRVGKVVPNHSENLRGIVMEVRLISSFHVNSVLVLLHALLVDSLLSLLGL 200
           GFQYQLGDFQLRVGKVVPN+SENLRGIVME                              
Sbjct: 114 GFQYQLGDFQLRVGKVVPNNSENLRGIVME------------------------------ 173

Query: 201 DSLMIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFKHIEPNFAEYGLSDYYTSQH 260
               IEYLPISSMEKSR+VMEEFFEVWQEAMSKSSLPGRF HIEPNFAEYGLSDYYTSQH
Sbjct: 174 ----IEYLPISSMEKSRKVMEEFFEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQH 223

Query: 261 TAVQYANVMAQLIATVQAVQAVRN 285
           TAVQYANVMAQLIATVQAVQ+ RN
Sbjct: 234 TAVQYANVMAQLIATVQAVQSARN 223

[Close]

BLAST of Spg003477 vs. NCBI nr
Match: XP_022946745.1 (mediator of RNA polymerase II transcription subunit 20a-like [Cucurbita moschata] >XP_022999230.1 mediator of RNA polymerase II transcription subunit 20a-like [Cucurbita maxima] >XP_023546929.1 mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023546930.1 mediator of RNA polymerase II transcription subunit 20a-like isoform X2 [Cucurbita pepo subsp. pepo] >KAG6599532.1 Mediator of RNA polymerase II transcription subunit 20a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 293.5 bits (750), Expect = 2.0e-75
Identity = 157/203 (77.34%), Postives = 163/203 (80.30%), Query Frame = 0


Query: 82  NIPAAEFPREFLGISLPEQPNKYYLIIRGQRIVLEADFSILTIMEKLQSYKLRVALNFEG 141
           NI A EFPR+FLGIS+PEQPNKYYLIIRGQRIVLEADF+I  IMEKLQSYKLRVALNFEG
Sbjct: 55  NIAAGEFPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEG 114

Query: 142 FQYQLGDFQLRVGKVVPNHSENLRGIVMEVRLISSFHVNSVLVLLHALLVDSLLSLLGLD 201
           FQYQLGDFQLRVGKVVPN+SENLRGIVME                               
Sbjct: 115 FQYQLGDFQLRVGKVVPNNSENLRGIVME------------------------------- 174

Query: 202 SLMIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFKHIEPNFAEYGLSDYYTSQHT 261
              IEYLPISSMEKS+QVMEEFFEVWQEAMSKSSLPGRF HIEPNFAEYGLSDYYTSQHT
Sbjct: 175 ---IEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHT 223

Query: 262 AVQYANVMAQLIATVQAVQAVRN 285
           AVQYANVMAQLIATVQAVQ+ RN
Sbjct: 235 AVQYANVMAQLIATVQAVQSARN 223

[Close]

BLAST of Spg003477 vs. NCBI nr
Match: KAG7030510.1 (Mediator of RNA polymerase II transcription subunit 20a, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 293.5 bits (750), Expect = 2.0e-75
Identity = 157/203 (77.34%), Postives = 163/203 (80.30%), Query Frame = 0


Query: 82  NIPAAEFPREFLGISLPEQPNKYYLIIRGQRIVLEADFSILTIMEKLQSYKLRVALNFEG 141
           NI A EFPR+FLGIS+PEQPNKYYLIIRGQRIVLEADF+I  IMEKLQSYKLRVALNFEG
Sbjct: 85  NIAAGEFPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEG 144

Query: 142 FQYQLGDFQLRVGKVVPNHSENLRGIVMEVRLISSFHVNSVLVLLHALLVDSLLSLLGLD 201
           FQYQLGDFQLRVGKVVPN+SENLRGIVME                               
Sbjct: 145 FQYQLGDFQLRVGKVVPNNSENLRGIVME------------------------------- 204

Query: 202 SLMIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFKHIEPNFAEYGLSDYYTSQHT 261
              IEYLPISSMEKS+QVMEEFFEVWQEAMSKSSLPGRF HIEPNFAEYGLSDYYTSQHT
Sbjct: 205 ---IEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHT 253

Query: 262 AVQYANVMAQLIATVQAVQAVRN 285
           AVQYANVMAQLIATVQAVQ+ RN
Sbjct: 265 AVQYANVMAQLIATVQAVQSARN 253

[Close]

BLAST of Spg003477 vs. NCBI nr
Match: XP_004141069.1 (mediator of RNA polymerase II transcription subunit 20a [Cucumis sativus] >KGN60509.1 hypothetical protein Csa_019466 [Cucumis sativus])

HSP 1 Score: 293.1 bits (749), Expect = 2.5e-75
Identity = 157/204 (76.96%), Postives = 163/204 (79.90%), Query Frame = 0


Query: 81  ENIPAAEFPREFLGISLPEQPNKYYLIIRGQRIVLEADFSILTIMEKLQSYKLRVALNFE 140
           +N PAAEFPR+FLGISLPEQPNKYYLIIRGQRIVLEADF+I  IMEKLQSYKLRVALNFE
Sbjct: 54  QNSPAAEFPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFE 113

Query: 141 GFQYQLGDFQLRVGKVVPNHSENLRGIVMEVRLISSFHVNSVLVLLHALLVDSLLSLLGL 200
           GFQYQLGDFQLRVGKVVPN+SENLRGIVME                              
Sbjct: 114 GFQYQLGDFQLRVGKVVPNNSENLRGIVME------------------------------ 173

Query: 201 DSLMIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFKHIEPNFAEYGLSDYYTSQH 260
               IEYLPISSMEKSRQVMEEFF+VWQEAMSKSSLPGRF HIE NFAEYGLSDYYTSQH
Sbjct: 174 ----IEYLPISSMEKSRQVMEEFFDVWQEAMSKSSLPGRFMHIESNFAEYGLSDYYTSQH 223

Query: 261 TAVQYANVMAQLIATVQAVQAVRN 285
           TAVQY NVMAQLIATVQAVQ+ RN
Sbjct: 234 TAVQYGNVMAQLIATVQAVQSARN 223

[Close]

BLAST of Spg003477 vs. NCBI nr
Match: XP_008459711.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucumis melo])

HSP 1 Score: 288.1 bits (736), Expect = 8.2e-74
Identity = 153/204 (75.00%), Postives = 162/204 (79.41%), Query Frame = 0


Query: 81  ENIPAAEFPREFLGISLPEQPNKYYLIIRGQRIVLEADFSILTIMEKLQSYKLRVALNFE 140
           +N PAAEFPR+FLGISLPEQPNKYYLIIRGQRIVLEADF+I  IMEKLQSYKLRVALNFE
Sbjct: 54  QNSPAAEFPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFE 113

Query: 141 GFQYQLGDFQLRVGKVVPNHSENLRGIVMEVRLISSFHVNSVLVLLHALLVDSLLSLLGL 200
           GFQYQLGDFQLRVGKVVPN+SENLRGIVME                              
Sbjct: 114 GFQYQLGDFQLRVGKVVPNNSENLRGIVME------------------------------ 173

Query: 201 DSLMIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFKHIEPNFAEYGLSDYYTSQH 260
               IEYLPISSMEKSRQ+M+EFF+VWQEAMSKSSLPGRF HIE NFAEYGLSD+YT QH
Sbjct: 174 ----IEYLPISSMEKSRQIMDEFFDVWQEAMSKSSLPGRFMHIESNFAEYGLSDFYTPQH 223

Query: 261 TAVQYANVMAQLIATVQAVQAVRN 285
           TAVQY NVMAQLIATVQAVQ+ RN
Sbjct: 234 TAVQYGNVMAQLIATVQAVQSARN 223

[Close]

BLAST of Spg003477 vs. ExPASy Swiss-Prot
Match: Q6NPF4 (Mediator of RNA polymerase II transcription subunit 20a OS=Arabidopsis thaliana OX=3702 GN=MED20A PE=1 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 2.5e-57
Identity = 121/194 (62.37%), Postives = 140/194 (72.16%), Query Frame = 0


Query: 87  EFPREFLGISLPEQPNKYYLIIRGQRIVLEADFSILTIMEKLQSYKLRVALNFEGFQYQL 146
           EFPR+FLGISL +QPNKYY IIR QRIVLEAD SI  IMEKLQSYK +VAL F+GFQYQL
Sbjct: 59  EFPRDFLGISLADQPNKYYFIIRTQRIVLEADSSIQLIMEKLQSYKSKVALYFDGFQYQL 118

Query: 147 GDFQLRVGKVVPNHSENLRGIVMEVRLISSFHVNSVLVLLHALLVDSLLSLLGLDSLMIE 206
           GDF+LRVGKVVP HSEN+RGIVMEV                                  E
Sbjct: 119 GDFRLRVGKVVPTHSENVRGIVMEV----------------------------------E 178

Query: 207 YLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFKHIEPNFAEYGLSDYYTSQHTAVQYA 266
           YLPISSMEK+++VMEEF E+W EA++K SLPG+F +I+ NF E+GL D YT QHTAV+YA
Sbjct: 179 YLPISSMEKAQKVMEEFLEIWNEALAKRSLPGKFVNIDLNFGEFGLGDIYTPQHTAVRYA 218

Query: 267 NVMAQLIATVQAVQ 281
            VMA +IATVQAV+
Sbjct: 239 LVMAHMIATVQAVR 218

[Close]

BLAST of Spg003477 vs. ExPASy Swiss-Prot
Match: Q9M0S0 (Mediator of RNA polymerase II transcription subunit 20b OS=Arabidopsis thaliana OX=3702 GN=MED20B PE=3 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 7.8e-51
Identity = 107/193 (55.44%), Postives = 131/193 (67.88%), Query Frame = 0


Query: 86  AEFPREFLGISLPEQPNKYYLIIRGQRIVLEADFSILTIMEKLQSYKLRVALNFEGFQYQ 145
           AEFPREF+GISLPE+P+KYY +IR QRIV+EAD SI  IME LQSYK +++  FEG +YQ
Sbjct: 58  AEFPREFVGISLPEEPDKYYFVIRSQRIVVEADSSIQMIMESLQSYKCKLSFYFEGLEYQ 117

Query: 146 LGDFQLRVGKVVPNHSENLRGIVMEVRLISSFHVNSVLVLLHALLVDSLLSLLGLDSLMI 205
           LGDF+LRVGKVVP H+E +RG+VMEV                                  
Sbjct: 118 LGDFRLRVGKVVPTHAETIRGVVMEV---------------------------------- 177

Query: 206 EYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFKHIEPNFAEYGLSDYYTSQHTAVQY 265
           EYLPISSM  ++++MEEF E+WQEAMSK SLPG+F + E NF ++GL D YT QHTAV Y
Sbjct: 178 EYLPISSMGMAKKLMEEFLEIWQEAMSKRSLPGKFVNKELNFEKFGLGDNYTPQHTAVGY 216

Query: 266 ANVMAQLIATVQA 279
           A  MA L+A +QA
Sbjct: 238 AFFMANLMAAIQA 216

[Close]

BLAST of Spg003477 vs. ExPASy Swiss-Prot
Match: Q54FL3 (Putative mediator of RNA polymerase II transcription subunit 20 OS=Dictyostelium discoideum OX=44689 GN=med20 PE=3 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.3e-05
Identity = 31/83 (37.35%), Postives = 48/83 (57.83%), Query Frame = 0


Query: 89  PREFLGISLPEQPNKYYLIIRGQRIVLEADFSILTIMEKLQSYKLRVALNFEGFQYQLGD 148
           PREF  +   E+P K + + R    ++E D  +++I+EK  SYK R        +Y+LGD
Sbjct: 50  PREFHLLLYDEKPKKCFTVCRD--TIVETDREMVSILEKTNSYKKRQTTEALISKYELGD 109

Query: 149 FQLRVGKVVPNHSENLRGIVMEV 172
           F +RVG +V      +RGI++EV
Sbjct: 110 FIIRVGPIV--FRTEIRGILVEV 128

[Close]

BLAST of Spg003477 vs. ExPASy TrEMBL
Match: A0A6J1KEU1 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita maxima OX=3661 GN=LOC111493666 PE=3 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 9.4e-76
Identity = 157/203 (77.34%), Postives = 163/203 (80.30%), Query Frame = 0


Query: 82  NIPAAEFPREFLGISLPEQPNKYYLIIRGQRIVLEADFSILTIMEKLQSYKLRVALNFEG 141
           NI A EFPR+FLGIS+PEQPNKYYLIIRGQRIVLEADF+I  IMEKLQSYKLRVALNFEG
Sbjct: 55  NIAAGEFPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEG 114

Query: 142 FQYQLGDFQLRVGKVVPNHSENLRGIVMEVRLISSFHVNSVLVLLHALLVDSLLSLLGLD 201
           FQYQLGDFQLRVGKVVPN+SENLRGIVME                               
Sbjct: 115 FQYQLGDFQLRVGKVVPNNSENLRGIVME------------------------------- 174

Query: 202 SLMIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFKHIEPNFAEYGLSDYYTSQHT 261
              IEYLPISSMEKS+QVMEEFFEVWQEAMSKSSLPGRF HIEPNFAEYGLSDYYTSQHT
Sbjct: 175 ---IEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHT 223

Query: 262 AVQYANVMAQLIATVQAVQAVRN 285
           AVQYANVMAQLIATVQAVQ+ RN
Sbjct: 235 AVQYANVMAQLIATVQAVQSARN 223

[Close]

BLAST of Spg003477 vs. ExPASy TrEMBL
Match: A0A6J1G4U3 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita moschata OX=3662 GN=LOC111450720 PE=3 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 9.4e-76
Identity = 157/203 (77.34%), Postives = 163/203 (80.30%), Query Frame = 0


Query: 82  NIPAAEFPREFLGISLPEQPNKYYLIIRGQRIVLEADFSILTIMEKLQSYKLRVALNFEG 141
           NI A EFPR+FLGIS+PEQPNKYYLIIRGQRIVLEADF+I  IMEKLQSYKLRVALNFEG
Sbjct: 55  NIAAGEFPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEG 114

Query: 142 FQYQLGDFQLRVGKVVPNHSENLRGIVMEVRLISSFHVNSVLVLLHALLVDSLLSLLGLD 201
           FQYQLGDFQLRVGKVVPN+SENLRGIVME                               
Sbjct: 115 FQYQLGDFQLRVGKVVPNNSENLRGIVME------------------------------- 174

Query: 202 SLMIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFKHIEPNFAEYGLSDYYTSQHT 261
              IEYLPISSMEKS+QVMEEFFEVWQEAMSKSSLPGRF HIEPNFAEYGLSDYYTSQHT
Sbjct: 175 ---IEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHT 223

Query: 262 AVQYANVMAQLIATVQAVQAVRN 285
           AVQYANVMAQLIATVQAVQ+ RN
Sbjct: 235 AVQYANVMAQLIATVQAVQSARN 223

[Close]

BLAST of Spg003477 vs. ExPASy TrEMBL
Match: A0A0A0LHQ1 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis sativus OX=3659 GN=MED20 PE=3 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 1.2e-75
Identity = 157/204 (76.96%), Postives = 163/204 (79.90%), Query Frame = 0


Query: 81  ENIPAAEFPREFLGISLPEQPNKYYLIIRGQRIVLEADFSILTIMEKLQSYKLRVALNFE 140
           +N PAAEFPR+FLGISLPEQPNKYYLIIRGQRIVLEADF+I  IMEKLQSYKLRVALNFE
Sbjct: 54  QNSPAAEFPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFE 113

Query: 141 GFQYQLGDFQLRVGKVVPNHSENLRGIVMEVRLISSFHVNSVLVLLHALLVDSLLSLLGL 200
           GFQYQLGDFQLRVGKVVPN+SENLRGIVME                              
Sbjct: 114 GFQYQLGDFQLRVGKVVPNNSENLRGIVME------------------------------ 173

Query: 201 DSLMIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFKHIEPNFAEYGLSDYYTSQH 260
               IEYLPISSMEKSRQVMEEFF+VWQEAMSKSSLPGRF HIE NFAEYGLSDYYTSQH
Sbjct: 174 ----IEYLPISSMEKSRQVMEEFFDVWQEAMSKSSLPGRFMHIESNFAEYGLSDYYTSQH 223

Query: 261 TAVQYANVMAQLIATVQAVQAVRN 285
           TAVQY NVMAQLIATVQAVQ+ RN
Sbjct: 234 TAVQYGNVMAQLIATVQAVQSARN 223

[Close]

BLAST of Spg003477 vs. ExPASy TrEMBL
Match: A0A5D3CH02 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo var. makuwa OX=1194695 GN=MED20 PE=3 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 4.0e-74
Identity = 153/204 (75.00%), Postives = 162/204 (79.41%), Query Frame = 0


Query: 81  ENIPAAEFPREFLGISLPEQPNKYYLIIRGQRIVLEADFSILTIMEKLQSYKLRVALNFE 140
           +N PAAEFPR+FLGISLPEQPNKYYLIIRGQRIVLEADF+I  IMEKLQSYKLRVALNFE
Sbjct: 102 QNSPAAEFPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFE 161

Query: 141 GFQYQLGDFQLRVGKVVPNHSENLRGIVMEVRLISSFHVNSVLVLLHALLVDSLLSLLGL 200
           GFQYQLGDFQLRVGKVVPN+SENLRGIVME                              
Sbjct: 162 GFQYQLGDFQLRVGKVVPNNSENLRGIVME------------------------------ 221

Query: 201 DSLMIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFKHIEPNFAEYGLSDYYTSQH 260
               IEYLPISSMEKSRQ+M+EFF+VWQEAMSKSSLPGRF HIE NFAEYGLSD+YT QH
Sbjct: 222 ----IEYLPISSMEKSRQIMDEFFDVWQEAMSKSSLPGRFMHIESNFAEYGLSDFYTPQH 271

Query: 261 TAVQYANVMAQLIATVQAVQAVRN 285
           TAVQY NVMAQLIATVQAVQ+ RN
Sbjct: 282 TAVQYGNVMAQLIATVQAVQSARN 271

[Close]

BLAST of Spg003477 vs. ExPASy TrEMBL
Match: A0A1S3CAW6 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo OX=3656 GN=LOC103498750 PE=3 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 4.0e-74
Identity = 153/204 (75.00%), Postives = 162/204 (79.41%), Query Frame = 0


Query: 81  ENIPAAEFPREFLGISLPEQPNKYYLIIRGQRIVLEADFSILTIMEKLQSYKLRVALNFE 140
           +N PAAEFPR+FLGISLPEQPNKYYLIIRGQRIVLEADF+I  IMEKLQSYKLRVALNFE
Sbjct: 54  QNSPAAEFPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFE 113

Query: 141 GFQYQLGDFQLRVGKVVPNHSENLRGIVMEVRLISSFHVNSVLVLLHALLVDSLLSLLGL 200
           GFQYQLGDFQLRVGKVVPN+SENLRGIVME                              
Sbjct: 114 GFQYQLGDFQLRVGKVVPNNSENLRGIVME------------------------------ 173

Query: 201 DSLMIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFKHIEPNFAEYGLSDYYTSQH 260
               IEYLPISSMEKSRQ+M+EFF+VWQEAMSKSSLPGRF HIE NFAEYGLSD+YT QH
Sbjct: 174 ----IEYLPISSMEKSRQIMDEFFDVWQEAMSKSSLPGRFMHIESNFAEYGLSDFYTPQH 223

Query: 261 TAVQYANVMAQLIATVQAVQAVRN 285
           TAVQY NVMAQLIATVQAVQ+ RN
Sbjct: 234 TAVQYGNVMAQLIATVQAVQSARN 223

[Close]

BLAST of Spg003477 vs. TAIR 10
Match: AT2G28230.1 (TATA-binding related factor (TRF) of subunit 20 of Mediator complex )

HSP 1 Score: 223.8 bits (569), Expect = 1.8e-58
Identity = 121/194 (62.37%), Postives = 140/194 (72.16%), Query Frame = 0


Query: 87  EFPREFLGISLPEQPNKYYLIIRGQRIVLEADFSILTIMEKLQSYKLRVALNFEGFQYQL 146
           EFPR+FLGISL +QPNKYY IIR QRIVLEAD SI  IMEKLQSYK +VAL F+GFQYQL
Sbjct: 59  EFPRDFLGISLADQPNKYYFIIRTQRIVLEADSSIQLIMEKLQSYKSKVALYFDGFQYQL 118

Query: 147 GDFQLRVGKVVPNHSENLRGIVMEVRLISSFHVNSVLVLLHALLVDSLLSLLGLDSLMIE 206
           GDF+LRVGKVVP HSEN+RGIVMEV                                  E
Sbjct: 119 GDFRLRVGKVVPTHSENVRGIVMEV----------------------------------E 178

Query: 207 YLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFKHIEPNFAEYGLSDYYTSQHTAVQYA 266
           YLPISSMEK+++VMEEF E+W EA++K SLPG+F +I+ NF E+GL D YT QHTAV+YA
Sbjct: 179 YLPISSMEKAQKVMEEFLEIWNEALAKRSLPGKFVNIDLNFGEFGLGDIYTPQHTAVRYA 218

Query: 267 NVMAQLIATVQAVQ 281
            VMA +IATVQAV+
Sbjct: 239 LVMAHMIATVQAVR 218

[Close]

BLAST of Spg003477 vs. TAIR 10
Match: AT4G09070.1 (TATA-binding related factor (TRF) of subunit 20 of Mediator complex )

HSP 1 Score: 202.2 bits (513), Expect = 5.5e-52
Identity = 107/193 (55.44%), Postives = 131/193 (67.88%), Query Frame = 0


Query: 86  AEFPREFLGISLPEQPNKYYLIIRGQRIVLEADFSILTIMEKLQSYKLRVALNFEGFQYQ 145
           AEFPREF+GISLPE+P+KYY +IR QRIV+EAD SI  IME LQSYK +++  FEG +YQ
Sbjct: 58  AEFPREFVGISLPEEPDKYYFVIRSQRIVVEADSSIQMIMESLQSYKCKLSFYFEGLEYQ 117

Query: 146 LGDFQLRVGKVVPNHSENLRGIVMEVRLISSFHVNSVLVLLHALLVDSLLSLLGLDSLMI 205
           LGDF+LRVGKVVP H+E +RG+VMEV                                  
Sbjct: 118 LGDFRLRVGKVVPTHAETIRGVVMEV---------------------------------- 177

Query: 206 EYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFKHIEPNFAEYGLSDYYTSQHTAVQY 265
           EYLPISSM  ++++MEEF E+WQEAMSK SLPG+F + E NF ++GL D YT QHTAV Y
Sbjct: 178 EYLPISSMGMAKKLMEEFLEIWQEAMSKRSLPGKFVNKELNFEKFGLGDNYTPQHTAVGY 216

Query: 266 ANVMAQLIATVQA 279
           A  MA L+A +QA
Sbjct: 238 AFFMANLMAAIQA 216

[Close]

BLAST of Spg003477 vs. TAIR 10
Match: AT2G28020.1 (BEST Arabidopsis thaliana protein match is: TATA-binding related factor (TRF) of subunit 20 of Mediator complex (TAIR:AT2G28230.1); Has 37 Blast hits to 37 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 37; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 82.4 bits (202), Expect = 6.4e-16
Identity = 43/68 (63.24%), Postives = 54/68 (79.41%), Query Frame = 0


Query: 213 MEKSRQVMEEFFEVWQEAMSKSSLPGRFKHIEPNFAEYGLSDYYTSQHTAVQ-YANVMAQ 272
           ME +R+VMEEF E+W EA++K SLPG+F + + NF E+GL D YT QHTAV+ YA VMA 
Sbjct: 1   MEIARKVMEEFLEIWNEALAKRSLPGKFVNKDLNFGEFGLGDNYTPQHTAVRNYALVMAH 60

Query: 273 LIATVQAV 280
           +IATVQAV
Sbjct: 61  MIATVQAV 68

The following BLAST results are available for this feature:

BLAST of Spg003477 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038889134.1	4.6e-77	77.94	mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Beninca...	[more]
XP_022946745.1	2.0e-75	77.34	mediator of RNA polymerase II transcription subunit 20a-like [Cucurbita moschata...	[more]
KAG7030510.1	2.0e-75	77.34	Mediator of RNA polymerase II transcription subunit 20a, partial [Cucurbita argy...	[more]
XP_004141069.1	2.5e-75	76.96	mediator of RNA polymerase II transcription subunit 20a [Cucumis sativus] >KGN60...	[more]
XP_008459711.1	8.2e-74	75.00	PREDICTED: mediator of RNA polymerase II transcription subunit 20a-like isoform ...	[more]

BLAST of Spg003477 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NPF4	2.5e-57	62.37	Mediator of RNA polymerase II transcription subunit 20a OS=Arabidopsis thaliana ...	[more]
Q9M0S0	7.8e-51	55.44	Mediator of RNA polymerase II transcription subunit 20b OS=Arabidopsis thaliana ...	[more]
Q54FL3	1.3e-05	37.35	Putative mediator of RNA polymerase II transcription subunit 20 OS=Dictyostelium...	[more]

BLAST of Spg003477 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KEU1	9.4e-76	77.34	Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita maxima OX=36...	[more]
A0A6J1G4U3	9.4e-76	77.34	Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita moschata OX=...	[more]
A0A0A0LHQ1	1.2e-75	76.96	Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis sativus OX=365...	[more]
A0A5D3CH02	4.0e-74	75.00	Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo var. maku...	[more]
A0A1S3CAW6	4.0e-74	75.00	Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo OX=3656 G...	[more]

BLAST of Spg003477 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28230.1	1.8e-58	62.37	TATA-binding related factor (TRF) of subunit 20 of Mediator complex	[more]
AT4G09070.1	5.5e-52	55.44	TATA-binding related factor (TRF) of subunit 20 of Mediator complex	[more]
AT2G28020.1	6.4e-16	63.24	BEST Arabidopsis thaliana protein match is: TATA-binding related factor (TRF) of...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013921	Mediator complex, subunit Med20	PFAM	PF08612	Med20	coord: 82..269 e-value: 1.3E-25 score: 90.1
IPR013921	Mediator complex, subunit Med20	PANTHER	PTHR12465	UBIQUITIN SPECIFIC PROTEASE HOMOLOG 49	coord: 205..278
IPR013921	Mediator complex, subunit Med20	PANTHER	PTHR12465	UBIQUITIN SPECIFIC PROTEASE HOMOLOG 49	coord: 83..173
None	No IPR available	PANTHER	PTHR12465:SF0	MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 20	coord: 83..173
None	No IPR available	PANTHER	PTHR12465:SF0	MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 20	coord: 205..278

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg003477.1	Spg003477.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045893	positive regulation of transcription, DNA-templated
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
cellular_component	GO:0016592	mediator complex
molecular_function	GO:0003713	transcription coactivator activity
molecular_function	GO:0003712	transcription coregulator activity