Spg003414 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg003414 vs. NCBI nr
Match: XP_038890330.1 (protein LAZ1 homolog 2 [Benincasa hispida])

HSP 1 Score: 708.4 bits (1827), Expect = 3.7e-200
Identity = 368/409 (89.98%), Postives = 383/409 (93.64%), Query Frame = 0

Query: 1   MKTSNSTSPQQDIYKDLYQPALVIAACFVVLALVLSIFLILHHLKSYSNPSEQKWIVAVL 60
           MKTS S +PQQDIY+DLYQPAL+IAACF V+ALVLS+FLIL HLKSYSNPSEQKWIVAVL
Sbjct: 1   MKTSISITPQQDIYEDLYQPALIIAACFAVVALVLSLFLILQHLKSYSNPSEQKWIVAVL 60

Query: 61  LMVPVYATESIISLWNPRFSLACDVLRSCYEAFALYSFGRYLIACLGGERRVVELLENES 120
            MVPVYATESIISLWNPRFSLACD+LR+CYEAFALYSFG YLIACLGGERRVVELLE+ES
Sbjct: 61  FMVPVYATESIISLWNPRFSLACDILRNCYEAFALYSFGSYLIACLGGERRVVELLESES 120

Query: 121 TKQLDEPLIEVEEKRSRSQRKLWNFLFKPRVLGKDLLTIEKFGLVQYMILKTTSAFLAFI 180
           T QLDEPLIE EEKRSR QR LWNFL KPR LGKDLLTIEKFGLVQYMILKT SAFLAFI
Sbjct: 121 TIQLDEPLIEGEEKRSRPQRTLWNFLLKPRALGKDLLTIEKFGLVQYMILKTASAFLAFI 180

Query: 181 LELSGVYGDGKFKWFYGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFK 240
           LEL GVYGDGKFKWFYGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFK
Sbjct: 181 LELFGVYGDGKFKWFYGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFK 240

Query: 241 AIVFATWWQGVGIALLRELGVLPKEGKLETGLQDFLICIEMAIAAVAHIFVFSAEPYRYI 300
           AIVFATWWQGVGIALLRELGVLPKEGKLETGLQDFLICIEMAIAAVAHIFVFSAEPYRYI
Sbjct: 241 AIVFATWWQGVGIALLRELGVLPKEGKLETGLQDFLICIEMAIAAVAHIFVFSAEPYRYI 300

Query: 301 PVSEYGVTIETVKEAAEVKEGK-EHKPTVVERTETHVEAPGTSVTESVQDIVLEGGQRVV 360
           PVS  GVTIETV+ A EVKEGK + KP +VERTETHVEAPGTSVTESVQDIVLEGGQRVV
Sbjct: 301 PVSASGVTIETVEGAVEVKEGKDDKKPALVERTETHVEAPGTSVTESVQDIVLEGGQRVV 360

Query: 361 KDVVLTINQAIRPVEKGVTRIQETLHQRTVDSDEKESDVEVEEYVKEIL 409
           KDVVLTINQAI PVEKGV  IQETLHQRT+ SDE+ESDVEVEEYV++ L
Sbjct: 361 KDVVLTINQAIGPVEKGVAMIQETLHQRTIGSDEEESDVEVEEYVEKTL 409

BLAST of Spg003414 vs. NCBI nr
Match: XP_023537814.1 (protein LAZ1 homolog 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 703.7 bits (1815), Expect = 9.0e-199
Identity = 365/408 (89.46%), Postives = 381/408 (93.38%), Query Frame = 0

Query: 1   MKTSNSTSPQQDIYKDLYQPALVIAACFVVLALVLSIFLILHHLKSYSNPSEQKWIVAVL 60
           M+T  S SPQQDIY DLYQPAL+IAACFVV+ALVLSIFLIL HLKSYS PSEQKWIVAVL
Sbjct: 1   MRTPISISPQQDIYGDLYQPALIIAACFVVVALVLSIFLILQHLKSYSKPSEQKWIVAVL 60

Query: 61  LMVPVYATESIISLWNPRFSLACDVLRSCYEAFALYSFGRYLIACLGGERRVVELLENES 120
            MVPVYATESIISLWNPRFSL CD+LR+CYEAFALYSFG YLIACLGGERRVVELLE ES
Sbjct: 61  FMVPVYATESIISLWNPRFSLVCDILRNCYEAFALYSFGSYLIACLGGERRVVELLEIES 120

Query: 121 TKQLDEPLIEVEEKRSRSQRKLWNFLFKPRVLGKDLLTIEKFGLVQYMILKTTSAFLAFI 180
           TKQL+EPLIE EEKRSRSQRKLWNF  KPRVLG+ LLTIEKFGLVQYMILKTTSAFLAFI
Sbjct: 121 TKQLEEPLIEGEEKRSRSQRKLWNFFLKPRVLGEHLLTIEKFGLVQYMILKTTSAFLAFI 180

Query: 181 LELSGVYGDGKFKWFYGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFK 240
           LEL GVYGDG+FKWFYGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFK
Sbjct: 181 LELFGVYGDGEFKWFYGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFK 240

Query: 241 AIVFATWWQGVGIALLRELGVLPKEGKLETGLQDFLICIEMAIAAVAHIFVFSAEPYRYI 300
           AIVFATWWQGVGIALLRELGVLPKEGKLETGLQDFLICIEMAIAA AH+FVFSAEPYRYI
Sbjct: 241 AIVFATWWQGVGIALLRELGVLPKEGKLETGLQDFLICIEMAIAAGAHMFVFSAEPYRYI 300

Query: 301 PVSEYGVTIETVKEAAEVKEGKEHKPTVVERTETHVEAPGTSVTESVQDIVLEGGQRVVK 360
           PVSEYGVTIE++K  AEVKEGKE KP VVE+TETHVEAPGTSV++SVQDIVLEGGQRVVK
Sbjct: 301 PVSEYGVTIESMKGRAEVKEGKEQKPAVVEKTETHVEAPGTSVSKSVQDIVLEGGQRVVK 360

Query: 361 DVVLTINQAIRPVEKGVTRIQETLHQRTVDSDEKESDVEVEEYVKEIL 409
           DVVLTINQAI PVEKGV  IQ TLHQ TV SDE+ESDVEVEEYV+E L
Sbjct: 361 DVVLTINQAIGPVEKGVAMIQGTLHQTTVGSDEEESDVEVEEYVQENL 408

BLAST of Spg003414 vs. NCBI nr
Match: KAG6586501.1 (Protein LAZ1-like 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 702.6 bits (1812), Expect = 2.0e-198
Identity = 365/408 (89.46%), Postives = 380/408 (93.14%), Query Frame = 0

Query: 1   MKTSNSTSPQQDIYKDLYQPALVIAACFVVLALVLSIFLILHHLKSYSNPSEQKWIVAVL 60
           MKT  S SPQQDIY DLYQPAL+IAACFVV+ALVLSIFLIL HLKSYS PSEQKWIVAVL
Sbjct: 1   MKTPISISPQQDIYGDLYQPALIIAACFVVVALVLSIFLILQHLKSYSKPSEQKWIVAVL 60

Query: 61  LMVPVYATESIISLWNPRFSLACDVLRSCYEAFALYSFGRYLIACLGGERRVVELLENES 120
            MVPVYATESIISLWNPRFSL CD+LR+CYEAFALYSFG YLIACLGGERRVVELLE ES
Sbjct: 61  FMVPVYATESIISLWNPRFSLVCDILRNCYEAFALYSFGSYLIACLGGERRVVELLEIES 120

Query: 121 TKQLDEPLIEVEEKRSRSQRKLWNFLFKPRVLGKDLLTIEKFGLVQYMILKTTSAFLAFI 180
           TKQL+EPLIE EEKRSRSQRKLWNF  KPRVLG+ LLTIEKFGLVQYMILKTTSAFLAFI
Sbjct: 121 TKQLEEPLIEGEEKRSRSQRKLWNFFLKPRVLGEHLLTIEKFGLVQYMILKTTSAFLAFI 180

Query: 181 LELSGVYGDGKFKWFYGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFK 240
           LEL GVYGDG+FKWFYGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFK
Sbjct: 181 LELFGVYGDGEFKWFYGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFK 240

Query: 241 AIVFATWWQGVGIALLRELGVLPKEGKLETGLQDFLICIEMAIAAVAHIFVFSAEPYRYI 300
           AIVFATWWQGVGIALLRELGVLPKEGKLETGLQDFLICIEMAIAA AH+FVFSAEPYRYI
Sbjct: 241 AIVFATWWQGVGIALLRELGVLPKEGKLETGLQDFLICIEMAIAAGAHMFVFSAEPYRYI 300

Query: 301 PVSEYGVTIETVKEAAEVKEGKEHKPTVVERTETHVEAPGTSVTESVQDIVLEGGQRVVK 360
           PVSEYGVTIE++K  A+VKEGKE KP VVE+TETHVEAPGTSV+ESVQDIVLEGGQRVVK
Sbjct: 301 PVSEYGVTIESMKGRAKVKEGKEQKPAVVEKTETHVEAPGTSVSESVQDIVLEGGQRVVK 360

Query: 361 DVVLTINQAIRPVEKGVTRIQETLHQRTVDSDEKESDVEVEEYVKEIL 409
           DVVLTINQAI PVEKGV  IQ TLHQ  V SDE+ESDVEVEEYV+E L
Sbjct: 361 DVVLTINQAIGPVEKGVAMIQGTLHQTRVGSDEEESDVEVEEYVQENL 408

BLAST of Spg003414 vs. NCBI nr
Match: KAG7021358.1 (Protein LAZ1-like 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 701.0 bits (1808), Expect = 5.8e-198
Identity = 364/408 (89.22%), Postives = 380/408 (93.14%), Query Frame = 0

Query: 1   MKTSNSTSPQQDIYKDLYQPALVIAACFVVLALVLSIFLILHHLKSYSNPSEQKWIVAVL 60
           MKT  S SPQQDIY DLYQPAL+IAACFVV+ALVLSIFLIL HLKSYS PSEQKWIVAVL
Sbjct: 1   MKTPISISPQQDIYGDLYQPALIIAACFVVVALVLSIFLILKHLKSYSKPSEQKWIVAVL 60

Query: 61  LMVPVYATESIISLWNPRFSLACDVLRSCYEAFALYSFGRYLIACLGGERRVVELLENES 120
            MVPVYATESIISLWNPRFSL CD+LR+CYEAFALYSFG YLIACLGGERRVVELLE ES
Sbjct: 61  FMVPVYATESIISLWNPRFSLVCDILRNCYEAFALYSFGSYLIACLGGERRVVELLEIES 120

Query: 121 TKQLDEPLIEVEEKRSRSQRKLWNFLFKPRVLGKDLLTIEKFGLVQYMILKTTSAFLAFI 180
           TKQL+EPLIE EEKRSRSQRKLWNF  KPRVLG+ LLTIEKFGLVQYMILKTTSAFLAFI
Sbjct: 121 TKQLEEPLIEGEEKRSRSQRKLWNFFLKPRVLGEHLLTIEKFGLVQYMILKTTSAFLAFI 180

Query: 181 LELSGVYGDGKFKWFYGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFK 240
           LEL GVYGDG+FKWFYGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFK
Sbjct: 181 LELFGVYGDGEFKWFYGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFK 240

Query: 241 AIVFATWWQGVGIALLRELGVLPKEGKLETGLQDFLICIEMAIAAVAHIFVFSAEPYRYI 300
           AIVFATWWQGVGIALLRELGVLPKEGKLETGLQDFLICIEMAIAA AH+FVFSAEPYRYI
Sbjct: 241 AIVFATWWQGVGIALLRELGVLPKEGKLETGLQDFLICIEMAIAAGAHMFVFSAEPYRYI 300

Query: 301 PVSEYGVTIETVKEAAEVKEGKEHKPTVVERTETHVEAPGTSVTESVQDIVLEGGQRVVK 360
           PVSEYGVTIE++K  A+VKEGKE KP VVE+TETHVEAPGTSV+ESVQD+VLEGGQRVVK
Sbjct: 301 PVSEYGVTIESMKGRAKVKEGKEQKPAVVEKTETHVEAPGTSVSESVQDMVLEGGQRVVK 360

Query: 361 DVVLTINQAIRPVEKGVTRIQETLHQRTVDSDEKESDVEVEEYVKEIL 409
           DVVLTINQAI PVEKGV  IQ TLHQ  V SDE+ESDVEVEEYV+E L
Sbjct: 361 DVVLTINQAIGPVEKGVAMIQGTLHQTRVGSDEEESDVEVEEYVQENL 408

BLAST of Spg003414 vs. NCBI nr
Match: XP_022937732.1 (protein LAZ1 homolog 2 [Cucurbita moschata])

HSP 1 Score: 699.1 bits (1803), Expect = 2.2e-197
Identity = 363/408 (88.97%), Postives = 380/408 (93.14%), Query Frame = 0

Query: 1   MKTSNSTSPQQDIYKDLYQPALVIAACFVVLALVLSIFLILHHLKSYSNPSEQKWIVAVL 60
           MKT  S SPQQDIY DLYQPAL+IAACFVV+ALVLSIFLIL HLKSYS PSEQKWIVAVL
Sbjct: 1   MKTPISISPQQDIYGDLYQPALIIAACFVVVALVLSIFLILQHLKSYSKPSEQKWIVAVL 60

Query: 61  LMVPVYATESIISLWNPRFSLACDVLRSCYEAFALYSFGRYLIACLGGERRVVELLENES 120
            MVPVYATESIISLWNPRFSL CD+LR+CYEAFALYSFG YLIACLGGERRVVELLE ES
Sbjct: 61  FMVPVYATESIISLWNPRFSLVCDILRNCYEAFALYSFGSYLIACLGGERRVVELLEIES 120

Query: 121 TKQLDEPLIEVEEKRSRSQRKLWNFLFKPRVLGKDLLTIEKFGLVQYMILKTTSAFLAFI 180
           TKQL+EPLIE EEKRSRSQRKLWNF  KPRVLG+ LLTIEKFGLVQYMILKTTSAFLAFI
Sbjct: 121 TKQLEEPLIEGEEKRSRSQRKLWNFFLKPRVLGEHLLTIEKFGLVQYMILKTTSAFLAFI 180

Query: 181 LELSGVYGDGKFKWFYGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFK 240
           LEL GVYGDG+FKWFYGYPY+AVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFK
Sbjct: 181 LELFGVYGDGEFKWFYGYPYLAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFK 240

Query: 241 AIVFATWWQGVGIALLRELGVLPKEGKLETGLQDFLICIEMAIAAVAHIFVFSAEPYRYI 300
           AIVFATWWQGVGIALLRELGVLPKEGKLETGLQDFLICIEMAIAA AH+FVFSAEPYR+I
Sbjct: 241 AIVFATWWQGVGIALLRELGVLPKEGKLETGLQDFLICIEMAIAAGAHMFVFSAEPYRFI 300

Query: 301 PVSEYGVTIETVKEAAEVKEGKEHKPTVVERTETHVEAPGTSVTESVQDIVLEGGQRVVK 360
           PVSEYGVTIE++K  A+VKEGKE K  VVE+TETHVEAPGTSV+ESVQDIVLEGGQRVVK
Sbjct: 301 PVSEYGVTIESMKGRAKVKEGKEQKRVVVEKTETHVEAPGTSVSESVQDIVLEGGQRVVK 360

Query: 361 DVVLTINQAIRPVEKGVTRIQETLHQRTVDSDEKESDVEVEEYVKEIL 409
           DVVLTINQAI PVEKGV  IQ TLHQ TV SDE+ESDVEVEEYV+E L
Sbjct: 361 DVVLTINQAIGPVEKGVAMIQGTLHQTTVGSDEEESDVEVEEYVQENL 408

BLAST of Spg003414 vs. ExPASy Swiss-Prot
Match: Q5BPZ5 (Protein LAZ1 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=At1g23070 PE=2 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 6.4e-147
Identity = 273/397 (68.77%), Postives = 317/397 (79.85%), Query Frame = 0

Query: 14  YKDLYQPALVIAACFVVLALVLSIFLILHHLKSYSNPSEQKWIVAVLLMVPVYATESIIS 73
           Y+DL+ P+L+I   F  +A+ LS++ IL HL+ Y+NP+EQKWIV+VL MVPVYATESIIS
Sbjct: 9   YRDLHLPSLIIGGSFATVAICLSLYSILQHLRFYTNPAEQKWIVSVLFMVPVYATESIIS 68

Query: 74  LWNPRFSLACDVLRSCYEAFALYSFGRYLIACLGGERRVVELLENESTKQLDEPLIEV-- 133
           L N +FSL CD+LR+CYEAFALYSFG YL+ACLGGERRVVE LENES K    PL+E   
Sbjct: 69  LSNSKFSLPCDILRNCYEAFALYSFGSYLVACLGGERRVVEYLENESKK----PLLEEGA 128

Query: 134 -EEKRSRSQRKLWNFLFKPRVLGKDLLTIEKFGLVQYMILKTTSAFLAFILELSGVYGDG 193
            E K+ + +   W FL  P VLG++L  IEKFGLVQYMILKT  AFL F+LEL GVYGDG
Sbjct: 129 NESKKKKKKNSFWKFLCDPYVLGRELFVIEKFGLVQYMILKTFCAFLTFLLELLGVYGDG 188

Query: 194 KFKWFYGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFKAIVFATWWQG 253
           +FKW+YGYPYI VVLNFSQMWAL+CLVQFYNVTHE+LK IKPLAKFISFKAIVFATWWQG
Sbjct: 189 EFKWYYGYPYIVVVLNFSQMWALFCLVQFYNVTHERLKEIKPLAKFISFKAIVFATWWQG 248

Query: 254 VGIALLRELGVLPKEGKLETGLQDFLICIEMAIAAVAHIFVFSAEPYRYIPVSEYG-VTI 313
            GIALL   G+LPKEG+ + GLQDFLICIEMAIAAVAH+FVF AEPY YIPVSE G +T 
Sbjct: 249 FGIALLCYYGILPKEGRFQNGLQDFLICIEMAIAAVAHLFVFPAEPYHYIPVSECGKITA 308

Query: 314 ETVKEAAEVKEGKEHKPTVVERTETHVEAPGTSVTESVQDIVLEGGQRVVKDVVLTINQA 373
           ET K   +++EG      +VE TET VEA GTS+ ESVQDIV++GGQ VVKDVVLTINQA
Sbjct: 309 ETSKTEVKLEEG-----GLVETTETQVEASGTSIKESVQDIVIDGGQHVVKDVVLTINQA 368

Query: 374 IRPVEKGVTRIQETLHQRTVDSDEKESDVEVEEYVKE 407
           I PVEKGVT+IQ+T+HQ+ +DSD KE     EE   E
Sbjct: 369 IGPVEKGVTKIQDTIHQKLLDSDGKEETEVTEEVTVE 396

BLAST of Spg003414 vs. ExPASy Swiss-Prot
Match: F4JTN2 (Protein LAZ1 OS=Arabidopsis thaliana OX=3702 GN=LAZ1 PE=1 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 3.0e-88
Identity = 177/393 (45.04%), Postives = 256/393 (65.14%), Query Frame = 0

Query: 21  ALVIAACFVVLALVLSIFLILHHLKSYSNPSEQKWIVAVLLMVPVYATESIISLWNPRFS 80
           A  +A  F+VL L LS+FL+  HL +Y NP EQK+++ V+LMVP Y+ ES  SL  P  S
Sbjct: 20  ASFMAGAFLVLTLSLSLFLVFDHLSTYKNPEEQKFLIGVILMVPCYSIESFASLVKPSIS 79

Query: 81  LACDVLRSCYEAFALYSFGRYLIACLGGERRVVELLENESTKQLDEPLIEVEEKRSRSQR 140
           + C +LR CYE+FA+Y FGRYL+AC+GGE R +E +E +  K    PL++ ++++   + 
Sbjct: 80  VDCGILRDCYESFAMYCFGRYLVACIGGEERTIEFMERQGRKSFKTPLLDHKDEKGIIKH 139

Query: 141 KL-WNFLFKPRVLGKDLLTIEKFGLVQYMILKTTSAFLAFILELSGVYGDGKFKWFYGYP 200
               N   KP  L      + KFG+VQYMI+K+ +A  A ILE  GVY +G+FKW  GYP
Sbjct: 140 PFPMNLFLKPWRLSPWFYQVVKFGIVQYMIIKSLTALTALILEAFGVYCEGEFKWGCGYP 199

Query: 201 YIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFKAIVFATWWQGVGIALLREL 260
           Y+AVVLNFSQ WALYCLVQFY  T ++L  I+PLAKF++FK+IVF TWWQGV IALL  L
Sbjct: 200 YLAVVLNFSQSWALYCLVQFYGATKDELAHIQPLAKFLTFKSIVFLTWWQGVAIALLSSL 259

Query: 261 GV----LPKEGKLETGLQDFLICIEMAIAAVAHIFVFSAEPYRYI------PVSEYG--V 320
           G+    + +  +L+T +QDF+ICIEM IA+V H++VF A+PY  +       VS  G   
Sbjct: 260 GLFKSSIAQSLQLKTSVQDFIICIEMGIASVVHLYVFPAKPYGLMGDRFTGSVSVLGDYA 319

Query: 321 TIETVKEAAEVKEGKEHKPTVVERTETHVE-APGTSVTESVQDIVLEGGQRVVKDVVLTI 380
           +++   +  E+++ +  +PT V      V+   G ++ ES++D+ + GG+ +VKDV  T+
Sbjct: 320 SVDCPIDPDEIRDSE--RPTKVRLPHPDVDIRSGMTIKESMRDVFVGGGEYIVKDVRFTV 379

Query: 381 NQAIRPVEKGVTRIQETLHQRTVDSDEKESDVE 400
            QA+ P+EK +T+  E LH+  +  + K+ D E
Sbjct: 380 TQAVEPMEKSITKFNEKLHK--ISQNIKKHDKE 408

BLAST of Spg003414 vs. ExPASy Swiss-Prot
Match: Q94CA0 (Protein LAZ1 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=At1g77220 PE=2 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 4.1e-85
Identity = 176/377 (46.68%), Postives = 239/377 (63.40%), Query Frame = 0

Query: 22  LVIAACFVVLALVLSIFLILHHLKSYSNPSEQKWIVAVLLMVPVYATESIISLWNPRFSL 81
           ++ A+ FVV+A++L ++LI  HL SY+ P EQK+++ ++LMVPVYA ES +SL N   + 
Sbjct: 43  ILSASVFVVIAILLPMYLIFEHLASYNQPEEQKFLIGLILMVPVYAVESFLSLVNSEAAF 102

Query: 82  ACDVLRSCYEAFALYSFGRYLIACLGGERRVVELLENESTKQLDEPLIEVEEKRSRSQRK 141
            C+V+R CYEAFALY F RYLIACL GE R +E +E ++      PL+E        +  
Sbjct: 103 NCEVIRDCYEAFALYCFERYLIACLDGEERTIEFMEQQTVITQSTPLLEGTCSYGVVEHP 162

Query: 142 L-WNFLFKPRVLGKDLLTIEKFGLVQYMILKTTSAFLAFILELSGVYGDGKFKWFYGYPY 201
              N   K   LG       K G+VQYMILK   A LA ILE  GVYG+GKF W YGYPY
Sbjct: 163 FPMNCFVKDWSLGPQFYHAVKIGIVQYMILKMICALLAMILEAFGVYGEGKFAWNYGYPY 222

Query: 202 IAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFKAIVFATWWQGVGIALLRELG 261
           +AVVLNFSQ WALYCLVQFYNV  ++L PIKPLAKF++FK+IVF TWWQG+ +A L  +G
Sbjct: 223 LAVVLNFSQTWALYCLVQFYNVIKDKLAPIKPLAKFLTFKSIVFLTWWQGIIVAFLFSMG 282

Query: 262 V----LPKEGKLETGLQDFLICIEMAIAAVAHIFVFSAEPY-------RYIPVSEYGVTI 321
           +    L KE  L+T +QD++ICIEM IAAV H++VF A PY       R + V     +I
Sbjct: 283 LVKGSLAKE--LKTRIQDYIICIEMGIAAVVHLYVFPAAPYKRGERCVRNVAVMSDYASI 342

Query: 322 ETVKEAAEVKEGKEHKPTVVERTETHVEAPGTSVTESVQDIVLEGGQRVVKDVVLTINQA 381
           +   +  EVK+ +    T   R +   +    +  +SV+D+VL  G+ +V D+  T++  
Sbjct: 343 DVPPDPEEVKDSERTTRTRYGRHDDREKR--LNFPQSVRDVVLGSGEIIVDDMRFTVSHV 402

Query: 382 IRPVEKGVTRIQETLHQ 387
           + PVE+G+ +I  T HQ
Sbjct: 403 VEPVERGIAKINRTFHQ 415

BLAST of Spg003414 vs. ExPASy Swiss-Prot
Match: Q9NVA4 (Transmembrane protein 184C OS=Homo sapiens OX=9606 GN=TMEM184C PE=1 SV=2)

HSP 1 Score: 185.3 bits (469), Expect = 1.4e-45
Identity = 106/296 (35.81%), Postives = 171/296 (57.77%), Query Frame = 0

Query: 11  QDIYKDLYQPALVIAACFVVLALVLSIFLILHHLKSYSNPSEQKWIVAVLLMVPVYATES 70
           Q +   ++  A  IA  F++L + +S+++IL HL  Y+ P  QK I+ +L MVP+Y+ +S
Sbjct: 38  QKLEVGIHTKAWFIAGIFLLLTIPISLWVILQHLVHYTQPELQKPIIRILWMVPIYSLDS 97

Query: 71  IISLWNPRFSLACDVLRSCYEAFALYSFGRYLIACLGGE-RRVVELLENESTKQLDEPLI 130
            I+L  P  ++  D  R CYEA+ +Y+F  +L   L      +V +LE +  ++   PL 
Sbjct: 98  WIALKYPGIAIYVDTCRECYEAYVIYNFMGFLTNYLTNRYPNLVLILEAKDQQKHFPPLC 157

Query: 131 EVEEKRSRSQRKLWNFLFKPRVLGKDLLTIEKFGLVQYMILKTTSAFLAFILELSGVYGD 190
                              P  +G+ LL   K G++QY +++  +  +A I EL G+Y +
Sbjct: 158 ----------------CCPPWAMGEVLLFRCKLGVLQYTVVRPFTTIVALICELLGIYDE 217

Query: 191 GKFKWFYGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFKAIVFATWWQ 250
           G F +   + Y+ ++ N SQ++A+YCL+ FY V  E+L PI+P+ KF+  K +VF ++WQ
Sbjct: 218 GNFSFSNAWTYLVIINNMSQLFAMYCLLLFYKVLKEELSPIQPVGKFLCVKLVVFVSFWQ 277

Query: 251 GVGIALLRELGVLPKEGKLE--------TGLQDFLICIEMAIAAVAHIFVFSAEPY 298
            V IALL ++GV+ ++   E        TGLQDF+ICIEM +AA+AH + FS +PY
Sbjct: 278 AVVIALLVKVGVISEKHTWEWQTVEAVATGLQDFIICIEMFLAAIAHHYTFSYKPY 317

BLAST of Spg003414 vs. ExPASy Swiss-Prot
Match: Q5RET6 (Transmembrane protein 184C OS=Pongo abelii OX=9601 GN=TMEM184C PE=2 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 1.4e-45
Identity = 106/296 (35.81%), Postives = 171/296 (57.77%), Query Frame = 0

Query: 11  QDIYKDLYQPALVIAACFVVLALVLSIFLILHHLKSYSNPSEQKWIVAVLLMVPVYATES 70
           Q +   ++  A  IA  F++L + +S+++IL HL  Y+ P  QK I+ +L MVP+Y+ +S
Sbjct: 38  QKLEVGIHTKAWFIAGIFLLLTIPISLWVILQHLVHYTQPELQKPIIRILWMVPIYSLDS 97

Query: 71  IISLWNPRFSLACDVLRSCYEAFALYSFGRYLIACLGGE-RRVVELLENESTKQLDEPLI 130
            I+L  P  ++  D  R CYEA+ +Y+F  +L   L      +V +LE +  ++   PL 
Sbjct: 98  WIALKYPGIAIYVDTCRECYEAYVIYNFMGFLTNYLTNRYPNLVLILEAKDQQKHFPPLC 157

Query: 131 EVEEKRSRSQRKLWNFLFKPRVLGKDLLTIEKFGLVQYMILKTTSAFLAFILELSGVYGD 190
                              P  +G+ LL   K G++QY +++  +  +A I EL G+Y +
Sbjct: 158 ----------------CCPPWAMGEVLLFRCKLGVLQYTVVRPFTTIVALICELLGIYDE 217

Query: 191 GKFKWFYGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFKAIVFATWWQ 250
           G F +   + Y+ ++ N SQ++A+YCL+ FY V  E+L PI+P+ KF+  K +VF ++WQ
Sbjct: 218 GNFSFSNAWTYLVIINNMSQLFAMYCLLLFYKVLKEELSPIQPVGKFLCVKLVVFVSFWQ 277

Query: 251 GVGIALLRELGVLPKEGKLE--------TGLQDFLICIEMAIAAVAHIFVFSAEPY 298
            V IALL ++GV+ ++   E        TGLQDF+ICIEM +AA+AH + FS +PY
Sbjct: 278 AVVIALLVKVGVISEKHTWEWQTVEAVATGLQDFIICIEMFLAAIAHHYTFSYKPY 317

BLAST of Spg003414 vs. ExPASy TrEMBL
Match: A0A6J1FC20 (protein LAZ1 homolog 2 OS=Cucurbita moschata OX=3662 GN=LOC111444043 PE=4 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 1.1e-197
Identity = 363/408 (88.97%), Postives = 380/408 (93.14%), Query Frame = 0

Query: 1   MKTSNSTSPQQDIYKDLYQPALVIAACFVVLALVLSIFLILHHLKSYSNPSEQKWIVAVL 60
           MKT  S SPQQDIY DLYQPAL+IAACFVV+ALVLSIFLIL HLKSYS PSEQKWIVAVL
Sbjct: 1   MKTPISISPQQDIYGDLYQPALIIAACFVVVALVLSIFLILQHLKSYSKPSEQKWIVAVL 60

Query: 61  LMVPVYATESIISLWNPRFSLACDVLRSCYEAFALYSFGRYLIACLGGERRVVELLENES 120
            MVPVYATESIISLWNPRFSL CD+LR+CYEAFALYSFG YLIACLGGERRVVELLE ES
Sbjct: 61  FMVPVYATESIISLWNPRFSLVCDILRNCYEAFALYSFGSYLIACLGGERRVVELLEIES 120

Query: 121 TKQLDEPLIEVEEKRSRSQRKLWNFLFKPRVLGKDLLTIEKFGLVQYMILKTTSAFLAFI 180
           TKQL+EPLIE EEKRSRSQRKLWNF  KPRVLG+ LLTIEKFGLVQYMILKTTSAFLAFI
Sbjct: 121 TKQLEEPLIEGEEKRSRSQRKLWNFFLKPRVLGEHLLTIEKFGLVQYMILKTTSAFLAFI 180

Query: 181 LELSGVYGDGKFKWFYGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFK 240
           LEL GVYGDG+FKWFYGYPY+AVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFK
Sbjct: 181 LELFGVYGDGEFKWFYGYPYLAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFK 240

Query: 241 AIVFATWWQGVGIALLRELGVLPKEGKLETGLQDFLICIEMAIAAVAHIFVFSAEPYRYI 300
           AIVFATWWQGVGIALLRELGVLPKEGKLETGLQDFLICIEMAIAA AH+FVFSAEPYR+I
Sbjct: 241 AIVFATWWQGVGIALLRELGVLPKEGKLETGLQDFLICIEMAIAAGAHMFVFSAEPYRFI 300

Query: 301 PVSEYGVTIETVKEAAEVKEGKEHKPTVVERTETHVEAPGTSVTESVQDIVLEGGQRVVK 360
           PVSEYGVTIE++K  A+VKEGKE K  VVE+TETHVEAPGTSV+ESVQDIVLEGGQRVVK
Sbjct: 301 PVSEYGVTIESMKGRAKVKEGKEQKRVVVEKTETHVEAPGTSVSESVQDIVLEGGQRVVK 360

Query: 361 DVVLTINQAIRPVEKGVTRIQETLHQRTVDSDEKESDVEVEEYVKEIL 409
           DVVLTINQAI PVEKGV  IQ TLHQ TV SDE+ESDVEVEEYV+E L
Sbjct: 361 DVVLTINQAIGPVEKGVAMIQGTLHQTTVGSDEEESDVEVEEYVQENL 408

BLAST of Spg003414 vs. ExPASy TrEMBL
Match: A0A6J1HQT0 (protein LAZ1 homolog 2 OS=Cucurbita maxima OX=3661 GN=LOC111465784 PE=4 SV=1)

HSP 1 Score: 676.8 bits (1745), Expect = 5.7e-191
Identity = 362/412 (87.86%), Postives = 374/412 (90.78%), Query Frame = 0

Query: 1   MKTSNSTSPQQDIYKDLYQPALVIAACFVVLALVLSIFLILHHLKSYSNPSEQKWIVAVL 60
           MKT  S SPQQDIY DLYQPAL+IAACFVV+ALVLSIFLIL HLKSYS PSEQKWIVAVL
Sbjct: 1   MKTPISISPQQDIYGDLYQPALIIAACFVVVALVLSIFLILQHLKSYSKPSEQKWIVAVL 60

Query: 61  LMVPVYATESIISLWNPRFSLACDVLRSCYEAFALYSFGRYLIACLGGERRVVELLENES 120
            MVPVYATESIISLWN RFSL CD+LR+CYEAFALYSFG YLIACLGGERRVVELLE ES
Sbjct: 61  FMVPVYATESIISLWNQRFSLECDILRNCYEAFALYSFGSYLIACLGGERRVVELLEIES 120

Query: 121 TKQLDEPLIEVEEKRSRSQRKLWNFLFKPRVLGKDLLTIEKFGLVQYMILKTTSAFLAFI 180
           TKQL+EPLIE EEK  RSQR LWNF  KPRVLG+ LLTIEKFGLVQYMILKTTSAFLAFI
Sbjct: 121 TKQLEEPLIEGEEK--RSQRTLWNFFLKPRVLGEHLLTIEKFGLVQYMILKTTSAFLAFI 180

Query: 181 LELSGVYGDGKFKWFYGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFK 240
           LEL GVYGDG+FKWFYGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFK
Sbjct: 181 LELFGVYGDGEFKWFYGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFK 240

Query: 241 AIVFATWWQGVGIALLRELGVLPKEGKLETGLQDFLICIEMAIAAVAHIFVFSAEPYRYI 300
           AIVFATWWQGVGIALLRELGVLPKEGKLETGLQDFLICIEMAIAA AHIFVFSAEPYRYI
Sbjct: 241 AIVFATWWQGVGIALLRELGVLPKEGKLETGLQDFLICIEMAIAAGAHIFVFSAEPYRYI 300

Query: 301 PVSEYGVTIETVKEAAEVKEGKEHKPTVVERTET--HVEAPGTSVTESVQDIVLEGGQRV 360
           PVSEYGVTIE++K  AEVKEGKE KP VVERTET  HVEAPGTSV ESVQDIVLEGGQRV
Sbjct: 301 PVSEYGVTIESMKGRAEVKEGKEQKPAVVERTETHVHVEAPGTSVGESVQDIVLEGGQRV 360

Query: 361 VKDVVLTINQAIRPVEKGVTRIQETLHQRTV--DSDEKESDVEVEEYVKEIL 409
           VKDVVLTINQAI PVEK V  IQ TLHQ  V   SDE+ESDVEVEEY++E L
Sbjct: 361 VKDVVLTINQAIGPVEKVVAMIQGTLHQTMVGSGSDEEESDVEVEEYMQENL 410

BLAST of Spg003414 vs. ExPASy TrEMBL
Match: A0A6J1DQ80 (protein LAZ1 homolog 2 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111023213 PE=4 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 1.8e-189
Identity = 353/407 (86.73%), Postives = 372/407 (91.40%), Query Frame = 0

Query: 3   TSNSTSPQQDIYKDLYQPALVIAACFVVLALVLSIFLILHHLKSYSNPSEQKWIVAVLLM 62
           TSN  SPQQ +Y+DLYQPALVIAACFVV+AL+LS FLIL HL+SYSNP EQKWIVAVL M
Sbjct: 16  TSNLISPQQHMYRDLYQPALVIAACFVVVALLLSTFLILQHLRSYSNPPEQKWIVAVLFM 75

Query: 63  VPVYATESIISLWNPRFSLACDVLRSCYEAFALYSFGRYLIACLGGERRVVELLENESTK 122
           VPVYATESIIS WNPRFSLACD+LR+CYEAFALYSFGRYLIACLGGERRV+ELLE ES  
Sbjct: 76  VPVYATESIISFWNPRFSLACDILRNCYEAFALYSFGRYLIACLGGERRVIELLEVESKN 135

Query: 123 QLDEPLIEVEEKRSRSQRKLWNFLFKPRVLGKDLLTIEKFGLVQYMILKTTSAFLAFILE 182
           QLDEPLIE EEKR +SQR LWNFL KPRVLG+ LLTIEKFGLVQYMILKT S FLAFILE
Sbjct: 136 QLDEPLIEGEEKRPKSQRTLWNFLLKPRVLGQKLLTIEKFGLVQYMILKTASTFLAFILE 195

Query: 183 LSGVYGDGKFKWFYGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFKAI 242
           L GVYGDGKFKW+YGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFKAI
Sbjct: 196 LFGVYGDGKFKWYYGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFKAI 255

Query: 243 VFATWWQGVGIALLRELGVLPKEGKLETGLQDFLICIEMAIAAVAHIFVFSAEPYRYIPV 302
           VFATWWQGVGIALLRELGVLP EGKLET LQDFLICIEMAIAAVAHIFVFSAEPYR+IPV
Sbjct: 256 VFATWWQGVGIALLRELGVLP-EGKLETELQDFLICIEMAIAAVAHIFVFSAEPYRHIPV 315

Query: 303 SEY-GVTIETVKEAAEVKEGKEHKPTVVERTETHVEAPGTSVTESVQDIVLEGGQRVVKD 362
           S Y GVTIETVK   EV+EG++ KP +VERTETHVEAPGTSVTESVQDIVLEGGQRVVKD
Sbjct: 316 SGYRGVTIETVKGVVEVEEGEDKKP-LVERTETHVEAPGTSVTESVQDIVLEGGQRVVKD 375

Query: 363 VVLTINQAIRPVEKGVTRIQETLHQRTVDSDEKESDVEVEEYVKEIL 409
           VVLTINQAI PVEKGV  IQET H+RTV SDE ESDVE+EEYV+E L
Sbjct: 376 VVLTINQAIGPVEKGVAMIQETFHKRTVGSDEDESDVEIEEYVEENL 420

BLAST of Spg003414 vs. ExPASy TrEMBL
Match: A0A6J1DSZ7 (protein LAZ1 homolog 2 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111023213 PE=4 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 1.8e-189
Identity = 353/407 (86.73%), Postives = 372/407 (91.40%), Query Frame = 0

Query: 3   TSNSTSPQQDIYKDLYQPALVIAACFVVLALVLSIFLILHHLKSYSNPSEQKWIVAVLLM 62
           TSN  SPQQ +Y+DLYQPALVIAACFVV+AL+LS FLIL HL+SYSNP EQKWIVAVL M
Sbjct: 2   TSNLISPQQHMYRDLYQPALVIAACFVVVALLLSTFLILQHLRSYSNPPEQKWIVAVLFM 61

Query: 63  VPVYATESIISLWNPRFSLACDVLRSCYEAFALYSFGRYLIACLGGERRVVELLENESTK 122
           VPVYATESIIS WNPRFSLACD+LR+CYEAFALYSFGRYLIACLGGERRV+ELLE ES  
Sbjct: 62  VPVYATESIISFWNPRFSLACDILRNCYEAFALYSFGRYLIACLGGERRVIELLEVESKN 121

Query: 123 QLDEPLIEVEEKRSRSQRKLWNFLFKPRVLGKDLLTIEKFGLVQYMILKTTSAFLAFILE 182
           QLDEPLIE EEKR +SQR LWNFL KPRVLG+ LLTIEKFGLVQYMILKT S FLAFILE
Sbjct: 122 QLDEPLIEGEEKRPKSQRTLWNFLLKPRVLGQKLLTIEKFGLVQYMILKTASTFLAFILE 181

Query: 183 LSGVYGDGKFKWFYGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFKAI 242
           L GVYGDGKFKW+YGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFKAI
Sbjct: 182 LFGVYGDGKFKWYYGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFKAI 241

Query: 243 VFATWWQGVGIALLRELGVLPKEGKLETGLQDFLICIEMAIAAVAHIFVFSAEPYRYIPV 302
           VFATWWQGVGIALLRELGVLP EGKLET LQDFLICIEMAIAAVAHIFVFSAEPYR+IPV
Sbjct: 242 VFATWWQGVGIALLRELGVLP-EGKLETELQDFLICIEMAIAAVAHIFVFSAEPYRHIPV 301

Query: 303 SEY-GVTIETVKEAAEVKEGKEHKPTVVERTETHVEAPGTSVTESVQDIVLEGGQRVVKD 362
           S Y GVTIETVK   EV+EG++ KP +VERTETHVEAPGTSVTESVQDIVLEGGQRVVKD
Sbjct: 302 SGYRGVTIETVKGVVEVEEGEDKKP-LVERTETHVEAPGTSVTESVQDIVLEGGQRVVKD 361

Query: 363 VVLTINQAIRPVEKGVTRIQETLHQRTVDSDEKESDVEVEEYVKEIL 409
           VVLTINQAI PVEKGV  IQET H+RTV SDE ESDVE+EEYV+E L
Sbjct: 362 VVLTINQAIGPVEKGVAMIQETFHKRTVGSDEDESDVEIEEYVEENL 406

BLAST of Spg003414 vs. ExPASy TrEMBL
Match: A0A1S4E1K4 (protein LAZ1 homolog 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498920 PE=4 SV=1)

HSP 1 Score: 657.5 bits (1695), Expect = 3.6e-185
Identity = 345/408 (84.56%), Postives = 366/408 (89.71%), Query Frame = 0

Query: 1   MKTSNS-TSPQQDIYKDLYQPALVIAACFVVLALVLSIFLILHHLKSYSNPSEQKWIVAV 60
           MKTS S T  Q+DIY DLYQPAL IA CF V+ALVLS+FLIL HLKSYSNPSEQKWIVAV
Sbjct: 8   MKTSISITLQQEDIYGDLYQPALTIATCFAVIALVLSLFLILQHLKSYSNPSEQKWIVAV 67

Query: 61  LLMVPVYATESIISLWNPRFSLACDVLRSCYEAFALYSFGRYLIACLGGERRVVELLENE 120
           L MVPVYAT+SIISLWN RFSLACD+LR+CYEAFALYSFGRYLIA LGGERRV+ELLE+E
Sbjct: 68  LFMVPVYATQSIISLWNSRFSLACDILRNCYEAFALYSFGRYLIASLGGERRVIELLESE 127

Query: 121 STKQLDEPLIEVEEKRSRSQRKLWNFLFKPRVLGKDLLTIEKFGLVQYMILKTTSAFLAF 180
           S KQLDEPLIE EEKRSRSQR LWNFL KP  +GKDLLTIEKFGLVQYMILKTT+AFLAF
Sbjct: 128 SIKQLDEPLIEGEEKRSRSQRTLWNFLLKPHAVGKDLLTIEKFGLVQYMILKTTTAFLAF 187

Query: 181 ILELSGVYGDGKFKWFYGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISF 240
           ILEL GVYGDGKFKW YGYPYIAVVLNFSQMWALYCLVQFYN THEQLKPIKPLAKFISF
Sbjct: 188 ILELFGVYGDGKFKWSYGYPYIAVVLNFSQMWALYCLVQFYNATHEQLKPIKPLAKFISF 247

Query: 241 KAIVFATWWQGVGIALLRELGVLPKEGKLETGLQDFLICIEMAIAAVAHIFVFSAEPYRY 300
           KAIVFATWWQGVGIALLREL VLP EGKLE GLQDFLIC+EMAIAAVAHIFV SAEPYRY
Sbjct: 248 KAIVFATWWQGVGIALLRELEVLPNEGKLEIGLQDFLICVEMAIAAVAHIFVLSAEPYRY 307

Query: 301 IPVSEYGV-TIETVKEAAEVKEGKEHKPTVVERTETHVEAPGTSVTESVQDIVLEGGQRV 360
           IPVS  GV  IETVK  AEVKEGK+ K  +VE+ ETHVEAPGTSVTESVQ+IVLEGGQRV
Sbjct: 308 IPVSACGVAAIETVKGTAEVKEGKDKKSALVEKMETHVEAPGTSVTESVQNIVLEGGQRV 367

Query: 361 VKDVVLTINQAIRPVEKGVTRIQETLHQRTVDSDEKESDVEVEEYVKE 407
           VKDVVLTINQAIRPV KG+  IQETLHQR V S+E E+ VE+EEY++E
Sbjct: 368 VKDVVLTINQAIRPVGKGMAMIQETLHQRRVSSEELEAGVEIEEYMEE 415

BLAST of Spg003414 vs. TAIR 10
Match: AT1G23070.1 (Protein of unknown function (DUF300) )

HSP 1 Score: 521.9 bits (1343), Expect = 4.5e-148
Identity = 273/397 (68.77%), Postives = 317/397 (79.85%), Query Frame = 0

Query: 14  YKDLYQPALVIAACFVVLALVLSIFLILHHLKSYSNPSEQKWIVAVLLMVPVYATESIIS 73
           Y+DL+ P+L+I   F  +A+ LS++ IL HL+ Y+NP+EQKWIV+VL MVPVYATESIIS
Sbjct: 9   YRDLHLPSLIIGGSFATVAICLSLYSILQHLRFYTNPAEQKWIVSVLFMVPVYATESIIS 68

Query: 74  LWNPRFSLACDVLRSCYEAFALYSFGRYLIACLGGERRVVELLENESTKQLDEPLIEV-- 133
           L N +FSL CD+LR+CYEAFALYSFG YL+ACLGGERRVVE LENES K    PL+E   
Sbjct: 69  LSNSKFSLPCDILRNCYEAFALYSFGSYLVACLGGERRVVEYLENESKK----PLLEEGA 128

Query: 134 -EEKRSRSQRKLWNFLFKPRVLGKDLLTIEKFGLVQYMILKTTSAFLAFILELSGVYGDG 193
            E K+ + +   W FL  P VLG++L  IEKFGLVQYMILKT  AFL F+LEL GVYGDG
Sbjct: 129 NESKKKKKKNSFWKFLCDPYVLGRELFVIEKFGLVQYMILKTFCAFLTFLLELLGVYGDG 188

Query: 194 KFKWFYGYPYIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFKAIVFATWWQG 253
           +FKW+YGYPYI VVLNFSQMWAL+CLVQFYNVTHE+LK IKPLAKFISFKAIVFATWWQG
Sbjct: 189 EFKWYYGYPYIVVVLNFSQMWALFCLVQFYNVTHERLKEIKPLAKFISFKAIVFATWWQG 248

Query: 254 VGIALLRELGVLPKEGKLETGLQDFLICIEMAIAAVAHIFVFSAEPYRYIPVSEYG-VTI 313
            GIALL   G+LPKEG+ + GLQDFLICIEMAIAAVAH+FVF AEPY YIPVSE G +T 
Sbjct: 249 FGIALLCYYGILPKEGRFQNGLQDFLICIEMAIAAVAHLFVFPAEPYHYIPVSECGKITA 308

Query: 314 ETVKEAAEVKEGKEHKPTVVERTETHVEAPGTSVTESVQDIVLEGGQRVVKDVVLTINQA 373
           ET K   +++EG      +VE TET VEA GTS+ ESVQDIV++GGQ VVKDVVLTINQA
Sbjct: 309 ETSKTEVKLEEG-----GLVETTETQVEASGTSIKESVQDIVIDGGQHVVKDVVLTINQA 368

Query: 374 IRPVEKGVTRIQETLHQRTVDSDEKESDVEVEEYVKE 407
           I PVEKGVT+IQ+T+HQ+ +DSD KE     EE   E
Sbjct: 369 IGPVEKGVTKIQDTIHQKLLDSDGKEETEVTEEVTVE 396

BLAST of Spg003414 vs. TAIR 10
Match: AT4G38360.2 (Protein of unknown function (DUF300) )

HSP 1 Score: 327.0 bits (837), Expect = 2.1e-89
Identity = 177/393 (45.04%), Postives = 256/393 (65.14%), Query Frame = 0

Query: 21  ALVIAACFVVLALVLSIFLILHHLKSYSNPSEQKWIVAVLLMVPVYATESIISLWNPRFS 80
           A  +A  F+VL L LS+FL+  HL +Y NP EQK+++ V+LMVP Y+ ES  SL  P  S
Sbjct: 20  ASFMAGAFLVLTLSLSLFLVFDHLSTYKNPEEQKFLIGVILMVPCYSIESFASLVKPSIS 79

Query: 81  LACDVLRSCYEAFALYSFGRYLIACLGGERRVVELLENESTKQLDEPLIEVEEKRSRSQR 140
           + C +LR CYE+FA+Y FGRYL+AC+GGE R +E +E +  K    PL++ ++++   + 
Sbjct: 80  VDCGILRDCYESFAMYCFGRYLVACIGGEERTIEFMERQGRKSFKTPLLDHKDEKGIIKH 139

Query: 141 KL-WNFLFKPRVLGKDLLTIEKFGLVQYMILKTTSAFLAFILELSGVYGDGKFKWFYGYP 200
               N   KP  L      + KFG+VQYMI+K+ +A  A ILE  GVY +G+FKW  GYP
Sbjct: 140 PFPMNLFLKPWRLSPWFYQVVKFGIVQYMIIKSLTALTALILEAFGVYCEGEFKWGCGYP 199

Query: 201 YIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFKAIVFATWWQGVGIALLREL 260
           Y+AVVLNFSQ WALYCLVQFY  T ++L  I+PLAKF++FK+IVF TWWQGV IALL  L
Sbjct: 200 YLAVVLNFSQSWALYCLVQFYGATKDELAHIQPLAKFLTFKSIVFLTWWQGVAIALLSSL 259

Query: 261 GV----LPKEGKLETGLQDFLICIEMAIAAVAHIFVFSAEPYRYI------PVSEYG--V 320
           G+    + +  +L+T +QDF+ICIEM IA+V H++VF A+PY  +       VS  G   
Sbjct: 260 GLFKSSIAQSLQLKTSVQDFIICIEMGIASVVHLYVFPAKPYGLMGDRFTGSVSVLGDYA 319

Query: 321 TIETVKEAAEVKEGKEHKPTVVERTETHVE-APGTSVTESVQDIVLEGGQRVVKDVVLTI 380
           +++   +  E+++ +  +PT V      V+   G ++ ES++D+ + GG+ +VKDV  T+
Sbjct: 320 SVDCPIDPDEIRDSE--RPTKVRLPHPDVDIRSGMTIKESMRDVFVGGGEYIVKDVRFTV 379

Query: 381 NQAIRPVEKGVTRIQETLHQRTVDSDEKESDVE 400
            QA+ P+EK +T+  E LH+  +  + K+ D E
Sbjct: 380 TQAVEPMEKSITKFNEKLHK--ISQNIKKHDKE 408

BLAST of Spg003414 vs. TAIR 10
Match: AT1G77220.1 (Protein of unknown function (DUF300) )

HSP 1 Score: 316.6 bits (810), Expect = 2.9e-86
Identity = 176/377 (46.68%), Postives = 239/377 (63.40%), Query Frame = 0

Query: 22  LVIAACFVVLALVLSIFLILHHLKSYSNPSEQKWIVAVLLMVPVYATESIISLWNPRFSL 81
           ++ A+ FVV+A++L ++LI  HL SY+ P EQK+++ ++LMVPVYA ES +SL N   + 
Sbjct: 43  ILSASVFVVIAILLPMYLIFEHLASYNQPEEQKFLIGLILMVPVYAVESFLSLVNSEAAF 102

Query: 82  ACDVLRSCYEAFALYSFGRYLIACLGGERRVVELLENESTKQLDEPLIEVEEKRSRSQRK 141
            C+V+R CYEAFALY F RYLIACL GE R +E +E ++      PL+E        +  
Sbjct: 103 NCEVIRDCYEAFALYCFERYLIACLDGEERTIEFMEQQTVITQSTPLLEGTCSYGVVEHP 162

Query: 142 L-WNFLFKPRVLGKDLLTIEKFGLVQYMILKTTSAFLAFILELSGVYGDGKFKWFYGYPY 201
              N   K   LG       K G+VQYMILK   A LA ILE  GVYG+GKF W YGYPY
Sbjct: 163 FPMNCFVKDWSLGPQFYHAVKIGIVQYMILKMICALLAMILEAFGVYGEGKFAWNYGYPY 222

Query: 202 IAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFKAIVFATWWQGVGIALLRELG 261
           +AVVLNFSQ WALYCLVQFYNV  ++L PIKPLAKF++FK+IVF TWWQG+ +A L  +G
Sbjct: 223 LAVVLNFSQTWALYCLVQFYNVIKDKLAPIKPLAKFLTFKSIVFLTWWQGIIVAFLFSMG 282

Query: 262 V----LPKEGKLETGLQDFLICIEMAIAAVAHIFVFSAEPY-------RYIPVSEYGVTI 321
           +    L KE  L+T +QD++ICIEM IAAV H++VF A PY       R + V     +I
Sbjct: 283 LVKGSLAKE--LKTRIQDYIICIEMGIAAVVHLYVFPAAPYKRGERCVRNVAVMSDYASI 342

Query: 322 ETVKEAAEVKEGKEHKPTVVERTETHVEAPGTSVTESVQDIVLEGGQRVVKDVVLTINQA 381
           +   +  EVK+ +    T   R +   +    +  +SV+D+VL  G+ +V D+  T++  
Sbjct: 343 DVPPDPEEVKDSERTTRTRYGRHDDREKR--LNFPQSVRDVVLGSGEIIVDDMRFTVSHV 402

Query: 382 IRPVEKGVTRIQETLHQ 387
           + PVE+G+ +I  T HQ
Sbjct: 403 VEPVERGIAKINRTFHQ 415

BLAST of Spg003414 vs. TAIR 10
Match: AT4G38360.1 (Protein of unknown function (DUF300) )

HSP 1 Score: 274.2 bits (700), Expect = 1.6e-73
Identity = 136/265 (51.32%), Postives = 183/265 (69.06%), Query Frame = 0

Query: 21  ALVIAACFVVLALVLSIFLILHHLKSYSNPSEQKWIVAVLLMVPVYATESIISLWNPRFS 80
           A  +A  F+VL L LS+FL+  HL +Y NP EQK+++ V+LMVP Y+ ES  SL  P  S
Sbjct: 20  ASFMAGAFLVLTLSLSLFLVFDHLSTYKNPEEQKFLIGVILMVPCYSIESFASLVKPSIS 79

Query: 81  LACDVLRSCYEAFALYSFGRYLIACLGGERRVVELLENESTKQLDEPLIEVEEKRSRSQR 140
           + C +LR CYE+FA+Y FGRYL+AC+GGE R +E +E +  K    PL++ ++++   + 
Sbjct: 80  VDCGILRDCYESFAMYCFGRYLVACIGGEERTIEFMERQGRKSFKTPLLDHKDEKGIIKH 139

Query: 141 KL-WNFLFKPRVLGKDLLTIEKFGLVQYMILKTTSAFLAFILELSGVYGDGKFKWFYGYP 200
               N   KP  L      + KFG+VQYMI+K+ +A  A ILE  GVY +G+FKW  GYP
Sbjct: 140 PFPMNLFLKPWRLSPWFYQVVKFGIVQYMIIKSLTALTALILEAFGVYCEGEFKWGCGYP 199

Query: 201 YIAVVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFKAIVFATWWQGVGIALLREL 260
           Y+AVVLNFSQ WALYCLVQFY  T ++L  I+PLAKF++FK+IVF TWWQGV IALL  L
Sbjct: 200 YLAVVLNFSQSWALYCLVQFYGATKDELAHIQPLAKFLTFKSIVFLTWWQGVAIALLSSL 259

Query: 261 GV----LPKEGKLETGLQDFLICIE 281
           G+    + +  +L+T +QDF+ICIE
Sbjct: 260 GLFKSSIAQSLQLKTSVQDFIICIE 284

BLAST of Spg003414 vs. TAIR 10
Match: AT5G26740.1 (Protein of unknown function (DUF300) )

HSP 1 Score: 150.2 bits (378), Expect = 3.6e-36
Identity = 97/298 (32.55%), Postives = 147/298 (49.33%), Query Frame = 0

Query: 23  VIAACFVVLALVLSIFLILHHLKSYSNPSEQKWIVAVLLMVPVYATESIISLWNPRFSLA 82
           ++A    V A+ L+IF I  HL +Y+ P+ Q++IV ++ MVPVYA  S +SL  P+ S+ 
Sbjct: 11  IVAFLCTVGAIALAIFHIYRHLLNYTEPTYQRYIVRIIFMVPVYAFMSFLSLVLPKSSIY 70

Query: 83  CDVLRSCYEAFALYSFGRYLIACLGGERRVVELLENESTKQLDEPLIEVEEKRSRSQRKL 142
            D +R  YEA+ +Y+F    +A +GG   VV  L   S K                   L
Sbjct: 71  FDSIREVYEAWVIYNFLSLCLAWVGGPGSVVLSLSGRSLK---------------PSWSL 130

Query: 143 WNFLFKPRVLGKDLLTIEKFGLVQYMILKTTSAFLAFILELSGVYGDGKFKWFYGYPYIA 202
               F P  L    +   K G +Q++ILK     +  +L   G Y DG F     Y Y+ 
Sbjct: 131 MTCCFPPLTLDGRFIRRCKQGCLQFVILKPILVAVTLVLYAKGKYKDGNFNPDQAYLYLT 190

Query: 203 VVLNFSQMWALYCLVQFYNVTHEQLKPIKPLAKFISFKAIVFATWWQGVGIALLRELGVL 262
           ++   S   ALY LV FY    + L+P  P+ KF+  K++VF T+WQGV + L  + G +
Sbjct: 191 IIYTISYTVALYALVLFYMACRDLLQPFNPVPKFVIIKSVVFLTYWQGVLVFLAAKSGFI 250

Query: 263 PKEGKLETGLQDFLICIEMAIAAVAHIFVFSAEPYRYIPVSEYGVTIETVKEAAEVKE 321
            K  +     Q+F+IC+EM IAA  H + F  + Y    V   G    ++  A ++ +
Sbjct: 251 -KSAEAAAHFQNFIICVEMLIAAACHFYAFPYKEYAGANVGGSGSFSGSLSHAVKLND 292