Homology
BLAST of Spg003338 vs. NCBI nr
Match:
KAG6586408.1 (Cytochrome P450 734A1, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 997.7 bits (2578), Expect = 4.0e-287
Identity = 500/546 (91.58%), Postives = 515/546 (94.32%), Query Frame = 0
Query: 1 MVGGFSFFEWLRVSILCCFLLLVALRAAVVLWWRPRKIEQHFFRQGIRGPPYRFFIGNVK 60
MVGG EW++VSIL CFL++VALRAAVVLWWRPR+IE HFFRQGIRGPPYRFFIGNVK
Sbjct: 1 MVGG--VLEWIKVSILGCFLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVK 60
Query: 61 ELVGMMIKASSDHSFPHFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIR 120
ELVGMMIKASSDHSFP+FSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIR
Sbjct: 61 ELVGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIR 120
Query: 121 EIFTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAK 180
EIFTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAK
Sbjct: 121 EIFTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAK 180
Query: 181 SVVDMLEKWSAMSAESGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVL 240
SVVDMLEKWSAMSA SGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVL
Sbjct: 181 SVVDMLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVL 240
Query: 241 AAQAFEKVFIPGYRYDDRHHYLNYYYYLTTTLRRFLPTRTNVNSWRLDKEIRKSLMKLIE 300
A+QAFEKVFIPGY RFLPTRTNVNSWRLD+EIRKSL+KLI+
Sbjct: 241 ASQAFEKVFIPGY--------------------RFLPTRTNVNSWRLDREIRKSLLKLID 300
Query: 301 RRRENSIESSKDLLGLMIRASNSCPSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILM 360
RRRE+SIESSKDLLGLMIRASN+ PSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILM
Sbjct: 301 RRRESSIESSKDLLGLMIRASNTSPSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILM 360
Query: 361 AMHPQWQVQARDEVLRECGARDIPSKDDVTKLKTLSMIVNESLRLYPPTVATIRQAKADV 420
AMHPQWQV+ARDEVLRECGARDIPSKD++TKLKTLSMIVNESLRLYPPTVATIRQAKADV
Sbjct: 361 AMHPQWQVKARDEVLRECGARDIPSKDNITKLKTLSMIVNESLRLYPPTVATIRQAKADV 420
Query: 421 ELGGYMIPRGTELLIPILAVHHDQKIWGNDVNEFNPGRFAEGVARAANHRVGFIPFGLGA 480
ELGGYMIPRGTELLIPILAVHHDQKIWGNDVNEFNPGRFAEGVARAANHRVGFIPFGLGA
Sbjct: 421 ELGGYMIPRGTELLIPILAVHHDQKIWGNDVNEFNPGRFAEGVARAANHRVGFIPFGLGA 480
Query: 481 RTCIGQNLAILQVKLALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKLSTPLI 540
RTCIGQNLAILQ KLALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKLSTP I
Sbjct: 481 RTCIGQNLAILQAKLALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKLSTPWI 524
Query: 541 HQDQRS 547
QDQRS
Sbjct: 541 QQDQRS 524
BLAST of Spg003338 vs. NCBI nr
Match:
XP_022965676.1 (cytochrome P450 734A1-like [Cucurbita maxima])
HSP 1 Score: 996.9 bits (2576), Expect = 6.9e-287
Identity = 500/546 (91.58%), Postives = 514/546 (94.14%), Query Frame = 0
Query: 1 MVGGFSFFEWLRVSILCCFLLLVALRAAVVLWWRPRKIEQHFFRQGIRGPPYRFFIGNVK 60
MVGG EW++VSIL CFL++VALRAAVVLWWRPRKIE HFFRQGIRGPPYRFFIGNVK
Sbjct: 1 MVGG--VLEWIKVSILGCFLVVVALRAAVVLWWRPRKIEDHFFRQGIRGPPYRFFIGNVK 60
Query: 61 ELVGMMIKASSDHSFPHFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIR 120
ELVGMMIKASSDHSFP+FSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIR
Sbjct: 61 ELVGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIR 120
Query: 121 EIFTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAK 180
EIFTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAK
Sbjct: 121 EIFTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAK 180
Query: 181 SVVDMLEKWSAMSAESGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVL 240
SVV+MLEKWSAMSA SGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVL
Sbjct: 181 SVVEMLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVL 240
Query: 241 AAQAFEKVFIPGYRYDDRHHYLNYYYYLTTTLRRFLPTRTNVNSWRLDKEIRKSLMKLIE 300
A+QAFEKVFIPGY RFLPTRTNVNSWRLD+EIRKSL+KLI+
Sbjct: 241 ASQAFEKVFIPGY--------------------RFLPTRTNVNSWRLDREIRKSLLKLID 300
Query: 301 RRRENSIESSKDLLGLMIRASNSCPSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILM 360
RRRE+SIESSKDLLGLMIRASN+ PSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILM
Sbjct: 301 RRRESSIESSKDLLGLMIRASNTSPSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILM 360
Query: 361 AMHPQWQVQARDEVLRECGARDIPSKDDVTKLKTLSMIVNESLRLYPPTVATIRQAKADV 420
AMHPQWQV+ARDEVLRECGARDIPSKDD+TKLKTLSMIVNESLRLYPPTVATIRQAKADV
Sbjct: 361 AMHPQWQVKARDEVLRECGARDIPSKDDITKLKTLSMIVNESLRLYPPTVATIRQAKADV 420
Query: 421 ELGGYMIPRGTELLIPILAVHHDQKIWGNDVNEFNPGRFAEGVARAANHRVGFIPFGLGA 480
ELGGYMIPRGTELLIPILAVHHDQKIWGNDVNEFNPGRF EGVARAANHRVGFIPFGLGA
Sbjct: 421 ELGGYMIPRGTELLIPILAVHHDQKIWGNDVNEFNPGRFTEGVARAANHRVGFIPFGLGA 480
Query: 481 RTCIGQNLAILQVKLALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKLSTPLI 540
RTCIGQNLAILQ KLALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKLSTP I
Sbjct: 481 RTCIGQNLAILQAKLALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKLSTPSI 524
Query: 541 HQDQRS 547
QDQRS
Sbjct: 541 PQDQRS 524
BLAST of Spg003338 vs. NCBI nr
Match:
XP_023537030.1 (cytochrome P450 734A1-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 995.3 bits (2572), Expect = 2.0e-286
Identity = 499/546 (91.39%), Postives = 513/546 (93.96%), Query Frame = 0
Query: 1 MVGGFSFFEWLRVSILCCFLLLVALRAAVVLWWRPRKIEQHFFRQGIRGPPYRFFIGNVK 60
MVGG EW++VSIL CFL++VALRAAVVLWWRPR+IE HFFRQGIRGPPYRFFIGNVK
Sbjct: 1 MVGG--VLEWIKVSILGCFLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVK 60
Query: 61 ELVGMMIKASSDHSFPHFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIR 120
ELVGMMIKASSDHSFP+FSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIR
Sbjct: 61 ELVGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIR 120
Query: 121 EIFTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAK 180
EIFTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAK
Sbjct: 121 EIFTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAK 180
Query: 181 SVVDMLEKWSAMSAESGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVL 240
SVVDMLEKWSAMSA SGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVL
Sbjct: 181 SVVDMLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVL 240
Query: 241 AAQAFEKVFIPGYRYDDRHHYLNYYYYLTTTLRRFLPTRTNVNSWRLDKEIRKSLMKLIE 300
A+QAFEKVFIPGY RFLPTRTNVNSWRLD+EIRKSL+KLI+
Sbjct: 241 ASQAFEKVFIPGY--------------------RFLPTRTNVNSWRLDREIRKSLLKLID 300
Query: 301 RRRENSIESSKDLLGLMIRASNSCPSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILM 360
RRRE+SIESSKDLLGLMIRASN+ PSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILM
Sbjct: 301 RRRESSIESSKDLLGLMIRASNTSPSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILM 360
Query: 361 AMHPQWQVQARDEVLRECGARDIPSKDDVTKLKTLSMIVNESLRLYPPTVATIRQAKADV 420
AMHPQWQV+ARDEVLRECGARDIPSKDD+TKLKTLSMIVNESLRLYPPTVATIRQAK DV
Sbjct: 361 AMHPQWQVKARDEVLRECGARDIPSKDDITKLKTLSMIVNESLRLYPPTVATIRQAKVDV 420
Query: 421 ELGGYMIPRGTELLIPILAVHHDQKIWGNDVNEFNPGRFAEGVARAANHRVGFIPFGLGA 480
ELGGYMIPRGTELLIPILAVHHDQKIWGNDVNEFNPGRFAEGVARAANHRVGFIPFGLGA
Sbjct: 421 ELGGYMIPRGTELLIPILAVHHDQKIWGNDVNEFNPGRFAEGVARAANHRVGFIPFGLGA 480
Query: 481 RTCIGQNLAILQVKLALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKLSTPLI 540
RTCIGQNLAILQ KLALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKLSTP I
Sbjct: 481 RTCIGQNLAILQAKLALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKLSTPSI 524
Query: 541 HQDQRS 547
Q QRS
Sbjct: 541 QQGQRS 524
BLAST of Spg003338 vs. NCBI nr
Match:
XP_022937875.1 (cytochrome P450 734A1-like [Cucurbita moschata])
HSP 1 Score: 994.2 bits (2569), Expect = 4.5e-286
Identity = 498/543 (91.71%), Postives = 512/543 (94.29%), Query Frame = 0
Query: 1 MVGGFSFFEWLRVSILCCFLLLVALRAAVVLWWRPRKIEQHFFRQGIRGPPYRFFIGNVK 60
MVGG EW++VSIL CFL++VALRAAVVLWWRPR+IE HFFRQGIRGPPYRFFIGNVK
Sbjct: 1 MVGG--VLEWIKVSILGCFLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVK 60
Query: 61 ELVGMMIKASSDHSFPHFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIR 120
ELVGMMIKASSDHSFP+FSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIR
Sbjct: 61 ELVGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIR 120
Query: 121 EIFTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAK 180
EIFTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAK
Sbjct: 121 EIFTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAK 180
Query: 181 SVVDMLEKWSAMSAESGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVL 240
SVVDMLEKWSAMSA SGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVL
Sbjct: 181 SVVDMLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVL 240
Query: 241 AAQAFEKVFIPGYRYDDRHHYLNYYYYLTTTLRRFLPTRTNVNSWRLDKEIRKSLMKLIE 300
A+QAFEKVFIPGY RFLPTRTNVNSWRLD+EIRKSL+KLI+
Sbjct: 241 ASQAFEKVFIPGY--------------------RFLPTRTNVNSWRLDREIRKSLLKLID 300
Query: 301 RRRENSIESSKDLLGLMIRASNSCPSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILM 360
RRRE+SIESSKDLLGLMIRASN+ PSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILM
Sbjct: 301 RRRESSIESSKDLLGLMIRASNTSPSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILM 360
Query: 361 AMHPQWQVQARDEVLRECGARDIPSKDDVTKLKTLSMIVNESLRLYPPTVATIRQAKADV 420
AMHPQWQV+ARDEVLRECGARDIPSKDD+TKLKTLSMIVNESLRLYPPTVATIRQAKADV
Sbjct: 361 AMHPQWQVKARDEVLRECGARDIPSKDDITKLKTLSMIVNESLRLYPPTVATIRQAKADV 420
Query: 421 ELGGYMIPRGTELLIPILAVHHDQKIWGNDVNEFNPGRFAEGVARAANHRVGFIPFGLGA 480
ELGGYMIPRGTELLIPILAVHHDQKIWGNDVNEFNPGRFAEGVARAANHRVGFIPFGLGA
Sbjct: 421 ELGGYMIPRGTELLIPILAVHHDQKIWGNDVNEFNPGRFAEGVARAANHRVGFIPFGLGA 480
Query: 481 RTCIGQNLAILQVKLALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKLSTPLI 540
RTCIGQNLAILQ KLALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKLSTP I
Sbjct: 481 RTCIGQNLAILQAKLALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKLSTPSI 521
Query: 541 HQD 544
QD
Sbjct: 541 QQD 521
BLAST of Spg003338 vs. NCBI nr
Match:
XP_038889459.1 (cytochrome P450 734A1 [Benincasa hispida])
HSP 1 Score: 965.3 bits (2494), Expect = 2.2e-277
Identity = 484/535 (90.47%), Postives = 501/535 (93.64%), Query Frame = 0
Query: 15 ILCCFLLLVALRAAVVLWWRPRKIEQHFFRQGIRGPPYRFFIGNVKELVGMMIKASSDHS 74
ILC FLLLV LRAAVVLWWRPR+IE+HFFRQGIRGPPYRFFIGNVKELVGMMIKASSDHS
Sbjct: 6 ILCWFLLLVVLRAAVVLWWRPRRIEEHFFRQGIRGPPYRFFIGNVKELVGMMIKASSDHS 65
Query: 75 FPHFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNE 134
FP+ SHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRL+VSDPDLIREIFTSKSEFCEKNE
Sbjct: 66 FPNSSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLSVSDPDLIREIFTSKSEFCEKNE 125
Query: 135 PHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSAM-- 194
PHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDM+EKWSA+
Sbjct: 126 PHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMMEKWSALMT 185
Query: 195 SAESGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAAQAFEKVFIPG 254
SA+SGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQM+LA+QAFEKVFIPG
Sbjct: 186 SADSGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMILASQAFEKVFIPG 245
Query: 255 YRYDDRHHYLNYYYYLTTTLRRFLPTRTNVNSWRLDKEIRKSLMKLIERRRENSIE-SSK 314
Y RFLPTRTNVNSWRLD+EIRKSLMKLI+RRRENSIE SSK
Sbjct: 246 Y--------------------RFLPTRTNVNSWRLDREIRKSLMKLIDRRRENSIETSSK 305
Query: 315 DLLGLMIRASNSCPSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILMAMHPQWQVQAR 374
DLLGLMIRAS S PSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILMAMHPQWQVQAR
Sbjct: 306 DLLGLMIRASKSSPSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILMAMHPQWQVQAR 365
Query: 375 DEVLRECGARDIPSKDDVTKLKTLSMIVNESLRLYPPTVATIRQAKADVELGGYMIPRGT 434
DEVLR CGARDIPSKDDVTKLK LSMI+NESLRLYPPTVATIRQAK DVELGGYMIPRGT
Sbjct: 366 DEVLRVCGARDIPSKDDVTKLKMLSMIINESLRLYPPTVATIRQAKVDVELGGYMIPRGT 425
Query: 435 ELLIPILAVHHDQKIWGNDVNEFNPGRFAEGVARAANHRVGFIPFGLGARTCIGQNLAIL 494
ELLIPILAVHHDQ IWGNDVNEFNPGRFAEGVA+AANHRVGFIPFGLGARTCIGQNLAIL
Sbjct: 426 ELLIPILAVHHDQTIWGNDVNEFNPGRFAEGVAKAANHRVGFIPFGLGARTCIGQNLAIL 485
Query: 495 QVKLALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKLSTPLIHQDQRS 547
Q KLALA+ILQRFSFRLGPSYQH+PAVQMLLYPR+GAPIIF KLSTPL+HQDQRS
Sbjct: 486 QAKLALALILQRFSFRLGPSYQHSPAVQMLLYPRHGAPIIFHKLSTPLLHQDQRS 520
BLAST of Spg003338 vs. ExPASy Swiss-Prot
Match:
O48786 (Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1)
HSP 1 Score: 765.8 bits (1976), Expect = 3.4e-220
Identity = 382/541 (70.61%), Postives = 444/541 (82.07%), Query Frame = 0
Query: 12 RVSILCCFLLLVALRAAVVLWWRPRKIEQHFFRQGIRGPPYRFFIGNVKELVGMMIKASS 71
+V +L L LV ++ +LWWRPRKIE+HF +QGIRGPPY FFIGNVKELVGMM+KASS
Sbjct: 12 KVLVLSVILSLVIVKGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKASS 71
Query: 72 DHSFPHFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCE 131
H P FSHNILPRVL FYHHW+KIYG+ FLVWFGPT RL V+DPDLIREIF SKSEF E
Sbjct: 72 -HPMP-FSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIF-SKSEFYE 131
Query: 132 KNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSA 191
KNE HPLVKQLEGDGLLSLKG+KWA HRKIISP+FHM+NLKLL+PV+ KSV DM++KWS
Sbjct: 132 KNEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSD 191
Query: 192 MSAESGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAAQAFEKVFIP 251
+E+GEVE++V EWFQ LTEDVI+RTAFGSSYEDG+A+FRLQAQQM+L A+AF+KVFIP
Sbjct: 192 KLSENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFIP 251
Query: 252 GYRYDDRHHYLNYYYYLTTTLRRFLPTRTNVNSWRLDKEIRKSLMKLIERRRENSIE--- 311
GY RF PTR N+ SW+LDKEIRKSL+KLIERRR+N+I+
Sbjct: 252 GY--------------------RFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEG 311
Query: 312 ------SSKDLLGLMIRASNSCPSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILMAM 371
++KDLLGLMI+A N +TV DIVEECK FFFAGKQTTSNLLTWT IL++M
Sbjct: 312 EECKEPAAKDLLGLMIQAKN------VTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSM 371
Query: 372 HPQWQVQARDEVLRECGARDIPSKDDVTKLKTLSMIVNESLRLYPPTVATIRQAKADVEL 431
HP+WQ +ARDEVLR CG+RD+P+KD V KLKTLSMI+NESLRLYPP VATIR+AK+DV+L
Sbjct: 372 HPEWQAKARDEVLRVCGSRDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKL 431
Query: 432 GGYMIPRGTELLIPILAVHHDQKIWGNDVNEFNPGRFAEGVARAANHRVGFIPFGLGART 491
GGY IP GTELLIPI+AVHHDQ IWGNDVNEFNP RFA+GV RAA H VGFIPFGLG RT
Sbjct: 432 GGYKIPCGTELLIPIIAVHHDQAIWGNDVNEFNPARFADGVPRAAKHPVGFIPFGLGVRT 491
Query: 492 CIGQNLAILQVKLALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKLSTPLIHQ 544
CIGQNLAILQ KL LA+++QRF+F L P+YQHAP V MLLYP++GAPI F++L+ H+
Sbjct: 492 CIGQNLAILQAKLTLAVMIQRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRLTN---HE 520
BLAST of Spg003338 vs. ExPASy Swiss-Prot
Match:
B9X287 (Cytochrome P450 734A6 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A6 PE=2 SV=1)
HSP 1 Score: 656.8 bits (1693), Expect = 2.2e-187
Identity = 320/532 (60.15%), Postives = 407/532 (76.50%), Query Frame = 0
Query: 23 VALRAAVVLWWRPRKIEQHFFRQGIRGPPYRFFIGNVKELVGMMIKASSDHSFPHF-SHN 82
VA++ VLWWRPR++E+HF RQGI GP YRF +G V+E+V +M+ AS+ P + SHN
Sbjct: 17 VAVKVLEVLWWRPRRVEEHFARQGITGPRYRFLVGCVREMVALMVAASAKPMPPPYRSHN 76
Query: 83 ILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNEPHPLVKQ 142
+LPRVL FYHHWKKIYGS FL+WFGPT RLA++DP+LIRE+ ++++ ++ E HP+V+Q
Sbjct: 77 VLPRVLAFYHHWKKIYGSTFLIWFGPTPRLAIADPELIREVLLARADRFDRYESHPMVRQ 136
Query: 143 LEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSAMSA--ESGEV 202
LEG+GL+SL+G KWA HR++++P+FHMDNL+LL+P + +V+DM +KW AM+ +SGEV
Sbjct: 137 LEGEGLVSLRGDKWAHHRRVLTPAFHMDNLRLLLPCVGMTVLDMADKWRAMAEADKSGEV 196
Query: 203 EIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAAQAFEKVFIPGYRYDDRH 262
EI+VS+WFQ +TED ITRTAFG SYEDGK +F+LQAQ M A++AF KVFIPGY
Sbjct: 197 EIDVSDWFQVVTEDAITRTAFGRSYEDGKVVFKLQAQLMAFASEAFRKVFIPGY------ 256
Query: 263 HYLNYYYYLTTTLRRFLPTRTNVNSWRLDKEIRKSLMKLIERRRENSIES-----SKDLL 322
RFLPT+ N +SW+LDKEIRK+L+ LI RR+E + +KDLL
Sbjct: 257 --------------RFLPTKKNTSSWKLDKEIRKNLVTLIGRRQEAGDDEKLDGCAKDLL 316
Query: 323 GLMIRAS--------NSCPSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILMAMHPQW 382
GLMI A+ ++ P S ITVNDIVEECK FFFAGKQTTSNLLTW ++++AMHP+W
Sbjct: 317 GLMINAAASSNGGKRSALPVSPITVNDIVEECKTFFFAGKQTTSNLLTWAIVVLAMHPEW 376
Query: 383 QVQARDEVLRECGARDIPSKDDVTKLKTLSMIVNESLRLYPPTVATIRQAKADVELGGYM 442
Q +AR EVL CGA +PS++ + KLKTL MI+NE+LRLYPP VAT+R+AKADVELGGY+
Sbjct: 377 QERARQEVLDVCGADGVPSREQLAKLKTLGMILNETLRLYPPAVATVRRAKADVELGGYL 436
Query: 443 -IPRGTELLIPILAVHHDQKIWGNDVNEFNPGRFAEGVARAANHRVGFIPFGLGARTCIG 502
IPR TELLIPI+AVHHD ++WG D +FNP RFA GVARAA H FIPFGLGAR CIG
Sbjct: 437 RIPRDTELLIPIMAVHHDARLWGPDAAQFNPARFAGGVARAARHPAAFIPFGLGARMCIG 496
Query: 503 QNLAILQVKLALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKLST 538
QNLAIL+ KL +A+IL RF FRL Y HAP V MLL+P+YGAPI+F+ S+
Sbjct: 497 QNLAILEAKLTVAVILHRFEFRLSARYVHAPTVLMLLHPQYGAPIVFRPRSS 528
BLAST of Spg003338 vs. ExPASy Swiss-Prot
Match:
Q6Z6D6 (Cytochrome P450 734A2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A2 PE=2 SV=1)
HSP 1 Score: 607.8 bits (1566), Expect = 1.2e-172
Identity = 295/537 (54.93%), Postives = 384/537 (71.51%), Query Frame = 0
Query: 23 VALRAAVVLWWRPRKIEQHFFRQGIRGPPYRFFIGNVKELVGMMIKASSDHSFPHFSHNI 82
VA R A LWWRPR++E HF QG+RGPPYRF +G+V+E+V +M +ASS P SHN
Sbjct: 33 VAARMADALWWRPRRLEAHFAAQGVRGPPYRFLLGSVREMVALMAEASSKPMSPPTSHNA 92
Query: 83 LPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNEPHPLVKQL 142
LPRVL FYH+W+KIYG +FL+WFGPT RL V++P+LIREIF ++++ ++ E HP+V+QL
Sbjct: 93 LPRVLAFYHYWRKIYGHRFLIWFGPTPRLTVAEPELIREIFLTRADAFDRYEAHPVVRQL 152
Query: 143 EGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSAM--SAESGEVE 202
EGDGL+SL G KWALHR++++ +F+ DNL LIP + KSV + KW AM + SGEVE
Sbjct: 153 EGDGLVSLHGDKWALHRRVLTDAFYPDNLNRLIPHVGKSVAALAAKWGAMAEAGGSGEVE 212
Query: 203 IEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAAQAFEKVFIPGYRYDDRHH 262
++V+EWFQ +TE+ ITR FG SY+DG+ +F +Q Q M A++AF KV +PGY
Sbjct: 213 VDVAEWFQAVTEEAITRATFGRSYDDGRVVFAMQGQLMAFASEAFRKVLVPGY------- 272
Query: 263 YLNYYYYLTTTLRRFLPTRTNVNSWRLDKEIRKSLMKLIERRRENSIESSK-------DL 322
RFLPT+ N SWRLD+EIR+SLM+LI RR + + + K DL
Sbjct: 273 -------------RFLPTKKNRLSWRLDREIRRSLMRLIGRRSDEAEQGEKADDGSFRDL 332
Query: 323 LGLMIRA------------SNSCPSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILMA 382
LGLMI A + P++ I V D++EECK FFFAGKQTT+NLLTW +L+A
Sbjct: 333 LGLMINAGAAAATRGNAGGEKNSPAAAIPVEDMLEECKTFFFAGKQTTTNLLTWATVLLA 392
Query: 383 MHPQWQVQARDEVLRECGARDIPSKDDVTKLKTLSMIVNESLRLYPPTVATIRQAKADVE 442
MHP WQ +AR EV CGA ++PSK+ + KLKTL MI+NE+LRLYPP VATIR+AK DV+
Sbjct: 393 MHPDWQERARREVFDVCGAGELPSKEHLPKLKTLGMIMNETLRLYPPAVATIRRAKVDVQ 452
Query: 443 LG-GYMIPRGTELLIPILAVHHDQKIWGNDVNEFNPGRFAEGVARAANHRVGFIPFGLGA 502
L G MIPR ELL+PI+A+HHD + WG D ++FNP RFA G ++AA H + FIPFGLG+
Sbjct: 453 LSDGCMIPRDMELLVPIMAIHHDTRYWGPDASQFNPARFANGASKAAKHPLAFIPFGLGS 512
Query: 503 RTCIGQNLAILQVKLALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKLST 538
R C+GQNLA L+ KL +AI+LQRF R P+Y HAP V MLLYP+YGAP+IF+ LS+
Sbjct: 513 RMCVGQNLARLEAKLTMAILLQRFEIRTSPNYVHAPTVLMLLYPQYGAPLIFRPLSS 549
BLAST of Spg003338 vs. ExPASy Swiss-Prot
Match:
Q69XM6 (Cytochrome P450 734A4 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A4 PE=2 SV=1)
HSP 1 Score: 580.9 bits (1496), Expect = 1.5e-164
Identity = 289/551 (52.45%), Postives = 380/551 (68.97%), Query Frame = 0
Query: 9 EWLRVSILCCFLLLVALRAAVVLWWRPRKIEQHFFRQGIRGPPYRFFIGNVKELVGMMIK 68
E + V+ LL VA R A +WWRPR++E HF QG+RGPPYRF +G V+E+V +M +
Sbjct: 3 EAVAVAAAVLLLLHVAARVADAVWWRPRRLEAHFAGQGVRGPPYRFLVGCVREMVALMAE 62
Query: 69 ASSDHSFPHFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSE 128
A++ P HN LPRVL FYH+W+KIYG FL+WFGPT RL V++P+++REIF +++E
Sbjct: 63 ATAKPMPPAAPHNALPRVLAFYHYWRKIYGPTFLIWFGPTPRLTVAEPEMVREIFLTRAE 122
Query: 129 FCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEK 188
++ E HP+V+QLEGDGL+SL G KWA HR++++P F+ DNL L+P + +SV + E+
Sbjct: 123 AFDRYEAHPVVRQLEGDGLVSLHGDKWAHHRRVLTPGFYPDNLNRLVPHVGRSVAALAER 182
Query: 189 WSAMS-AESGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAAQAFEK 248
W AM+ A GEVE++V+EWFQ + E+ ITR FG SY+ G+ +FRLQA+ M A++AF K
Sbjct: 183 WRAMACAGGGEVEVDVAEWFQAVAEEAITRATFGRSYDSGRVVFRLQARLMAFASEAFRK 242
Query: 249 VFIPGYRYDDRHHYLNYYYYLTTTLRRFLPTRTNVNSWRLDKEIRKSLMKLIERR----- 308
V +PGY RFLPT+ N SW LD+EIR+ L++LI RR
Sbjct: 243 VLVPGY--------------------RFLPTKKNRMSWGLDREIRRGLVRLIGRRSGGDG 302
Query: 309 ----------RENSIESSKDLLGLMIRASNSCPSSTITVNDIVEECKGFFFAGKQTTSNL 368
++ DLLGLMI N+ T+ V D+VEECK FFFAGKQTT+NL
Sbjct: 303 GEEDETTTELKDKQDSGFNDLLGLMI---NAGVDRTMPVEDMVEECKTFFFAGKQTTTNL 362
Query: 369 LTWTMILMAMHPQWQVQARDEVLRECG--ARDIPSKDDVTKLKTLSMIVNESLRLYPPTV 428
LTW +L+AMHP WQ +AR EVL CG A ++P+KD + KLKTL MI+NE+LRLYPP V
Sbjct: 363 LTWATVLLAMHPDWQDRARREVLAVCGDAAGELPTKDHLPKLKTLGMILNETLRLYPPAV 422
Query: 429 ATIRQAKADVEL--------GGYMIPRGTELLIPILAVHHDQKIWGNDVNEFNPGRFAEG 488
ATIR+AK DV L GG IPR TELL+PI+A+HHD ++WG D +FNP RFA G
Sbjct: 423 ATIRRAKFDVTLGGGGDGDAGGIHIPRDTELLVPIMAIHHDARLWGPDAAQFNPARFASG 482
Query: 489 VARAANHRVGFIPFGLGARTCIGQNLAILQVKLALAIILQRFSFRLGPSYQHAPAVQMLL 534
ARAA H + FIPFGLG+R CIGQ+LAIL+ KL +A++LQRF L P+Y HAP V MLL
Sbjct: 483 AARAAKHPLAFIPFGLGSRMCIGQSLAILEAKLTMAVLLQRFDLALSPTYVHAPTVLMLL 530
BLAST of Spg003338 vs. ExPASy Swiss-Prot
Match:
Q8LIF2 (Cytochrome P450 734A5 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A5 PE=2 SV=1)
HSP 1 Score: 523.9 bits (1348), Expect = 2.2e-147
Identity = 257/523 (49.14%), Postives = 357/523 (68.26%), Query Frame = 0
Query: 24 ALRAAVVLWWRPRKIEQHFFRQGIRGPPYRFFIGNVKELVGMMIKASSDHSFPHFSHNIL 83
A RAA LWWRPR++E+HF QG+RGP YRFF+G+ ELV +M+ A+S P SH+IL
Sbjct: 32 AARAAEALWWRPRRVERHFAAQGVRGPGYRFFVGSSIELVRLMVDAASRPMEPPTSHDIL 91
Query: 84 PRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNEPHPLVKQLE 143
PRVLPFYHHW+K+YG L+WFG T RL VS+P+LIRE+ ++++ ++ E HP++ Q E
Sbjct: 92 PRVLPFYHHWRKLYGPMHLIWFGRTPRLVVSEPELIREVLLTRADHFDRYEAHPMICQFE 151
Query: 144 GDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWS--AMSAESGEVEI 203
G GL +L G++WA R++++P+FH +NL+++ P +A +V ML++ + A + +GE E+
Sbjct: 152 GYGLSNLHGERWARRRRVLTPAFHTENLRMIAPFVAGTVTRMLDELAERARAGGAGEAEV 211
Query: 204 EVSEWFQTLTEDVITRTAFG-SSYEDGKAIFRLQAQQMVLAAQAFEKVFIPGYRYDDRHH 263
+V+EWFQ + ++ IT AFG +Y+DG A+FRLQ + A +A KV+IPGY
Sbjct: 212 DVAEWFQRVPQEAITFAAFGRRNYDDGAAVFRLQDELAGYATEAHSKVYIPGY------- 271
Query: 264 YLNYYYYLTTTLRRFLPTRTNVNSWRLDKEIRKSLMKLIERRRENSIESSKDL-----LG 323
RFLPTR N W+LD+EIR L K + + S D G
Sbjct: 272 -------------RFLPTRKNRRVWQLDREIRSHLAKFVTGLQSCSSSHGDDADDGGDGG 331
Query: 324 LMIRASNSCPSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILMAMHPQWQVQARDEVL 383
+R S + +T +I+EE K FFFAGK+T SNLLTWT + +AMHP+WQ +AR EV+
Sbjct: 332 GGMREFMSFMAPAMTAGEIIEESKNFFFAGKETLSNLLTWTTVALAMHPEWQERARREVV 391
Query: 384 RECGARDIPSKDDVTKLKTLSMIVNESLRLYPPTVATIRQAKADVELGGYMIPRGTELLI 443
CG D+P+KD + KLKTL MI+NE+LRLYPP VA IR AK DVELGG ++P GTE++I
Sbjct: 392 AVCGRGDLPTKDHLPKLKTLGMILNETLRLYPPAVAMIRTAKEDVELGGCVVPAGTEVMI 451
Query: 444 PILAVHHDQKIWGNDVNEFNPGRF-AEGVARAANHRVGFIPFGLGARTCIGQNLAILQVK 503
PI+AVHHD WG+D EFNP RF A+ H + F+PFG GAR CIGQN+A+++ K
Sbjct: 452 PIMAVHHDAAAWGDDAAEFNPARFAADDDGGRRRHPMAFMPFGGGARVCIGQNMALMEAK 511
Query: 504 LALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKLST 538
+ALA++L+RF FRL P+Y HAP V M+L P++GAP+IF+ L++
Sbjct: 512 VALAVVLRRFEFRLSPAYVHAPRVLMILSPQFGAPVIFRPLTS 534
BLAST of Spg003338 vs. ExPASy TrEMBL
Match:
A0A6J1HMB5 (cytochrome P450 734A1-like OS=Cucurbita maxima OX=3661 GN=LOC111465504 PE=3 SV=1)
HSP 1 Score: 996.9 bits (2576), Expect = 3.3e-287
Identity = 500/546 (91.58%), Postives = 514/546 (94.14%), Query Frame = 0
Query: 1 MVGGFSFFEWLRVSILCCFLLLVALRAAVVLWWRPRKIEQHFFRQGIRGPPYRFFIGNVK 60
MVGG EW++VSIL CFL++VALRAAVVLWWRPRKIE HFFRQGIRGPPYRFFIGNVK
Sbjct: 1 MVGG--VLEWIKVSILGCFLVVVALRAAVVLWWRPRKIEDHFFRQGIRGPPYRFFIGNVK 60
Query: 61 ELVGMMIKASSDHSFPHFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIR 120
ELVGMMIKASSDHSFP+FSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIR
Sbjct: 61 ELVGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIR 120
Query: 121 EIFTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAK 180
EIFTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAK
Sbjct: 121 EIFTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAK 180
Query: 181 SVVDMLEKWSAMSAESGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVL 240
SVV+MLEKWSAMSA SGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVL
Sbjct: 181 SVVEMLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVL 240
Query: 241 AAQAFEKVFIPGYRYDDRHHYLNYYYYLTTTLRRFLPTRTNVNSWRLDKEIRKSLMKLIE 300
A+QAFEKVFIPGY RFLPTRTNVNSWRLD+EIRKSL+KLI+
Sbjct: 241 ASQAFEKVFIPGY--------------------RFLPTRTNVNSWRLDREIRKSLLKLID 300
Query: 301 RRRENSIESSKDLLGLMIRASNSCPSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILM 360
RRRE+SIESSKDLLGLMIRASN+ PSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILM
Sbjct: 301 RRRESSIESSKDLLGLMIRASNTSPSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILM 360
Query: 361 AMHPQWQVQARDEVLRECGARDIPSKDDVTKLKTLSMIVNESLRLYPPTVATIRQAKADV 420
AMHPQWQV+ARDEVLRECGARDIPSKDD+TKLKTLSMIVNESLRLYPPTVATIRQAKADV
Sbjct: 361 AMHPQWQVKARDEVLRECGARDIPSKDDITKLKTLSMIVNESLRLYPPTVATIRQAKADV 420
Query: 421 ELGGYMIPRGTELLIPILAVHHDQKIWGNDVNEFNPGRFAEGVARAANHRVGFIPFGLGA 480
ELGGYMIPRGTELLIPILAVHHDQKIWGNDVNEFNPGRF EGVARAANHRVGFIPFGLGA
Sbjct: 421 ELGGYMIPRGTELLIPILAVHHDQKIWGNDVNEFNPGRFTEGVARAANHRVGFIPFGLGA 480
Query: 481 RTCIGQNLAILQVKLALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKLSTPLI 540
RTCIGQNLAILQ KLALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKLSTP I
Sbjct: 481 RTCIGQNLAILQAKLALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKLSTPSI 524
Query: 541 HQDQRS 547
QDQRS
Sbjct: 541 PQDQRS 524
BLAST of Spg003338 vs. ExPASy TrEMBL
Match:
A0A6J1FI14 (cytochrome P450 734A1-like OS=Cucurbita moschata OX=3662 GN=LOC111444133 PE=3 SV=1)
HSP 1 Score: 994.2 bits (2569), Expect = 2.2e-286
Identity = 498/543 (91.71%), Postives = 512/543 (94.29%), Query Frame = 0
Query: 1 MVGGFSFFEWLRVSILCCFLLLVALRAAVVLWWRPRKIEQHFFRQGIRGPPYRFFIGNVK 60
MVGG EW++VSIL CFL++VALRAAVVLWWRPR+IE HFFRQGIRGPPYRFFIGNVK
Sbjct: 1 MVGG--VLEWIKVSILGCFLVVVALRAAVVLWWRPRRIEDHFFRQGIRGPPYRFFIGNVK 60
Query: 61 ELVGMMIKASSDHSFPHFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIR 120
ELVGMMIKASSDHSFP+FSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIR
Sbjct: 61 ELVGMMIKASSDHSFPNFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIR 120
Query: 121 EIFTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAK 180
EIFTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAK
Sbjct: 121 EIFTSKSEFCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAK 180
Query: 181 SVVDMLEKWSAMSAESGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVL 240
SVVDMLEKWSAMSA SGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVL
Sbjct: 181 SVVDMLEKWSAMSAASGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVL 240
Query: 241 AAQAFEKVFIPGYRYDDRHHYLNYYYYLTTTLRRFLPTRTNVNSWRLDKEIRKSLMKLIE 300
A+QAFEKVFIPGY RFLPTRTNVNSWRLD+EIRKSL+KLI+
Sbjct: 241 ASQAFEKVFIPGY--------------------RFLPTRTNVNSWRLDREIRKSLLKLID 300
Query: 301 RRRENSIESSKDLLGLMIRASNSCPSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILM 360
RRRE+SIESSKDLLGLMIRASN+ PSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILM
Sbjct: 301 RRRESSIESSKDLLGLMIRASNTSPSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILM 360
Query: 361 AMHPQWQVQARDEVLRECGARDIPSKDDVTKLKTLSMIVNESLRLYPPTVATIRQAKADV 420
AMHPQWQV+ARDEVLRECGARDIPSKDD+TKLKTLSMIVNESLRLYPPTVATIRQAKADV
Sbjct: 361 AMHPQWQVKARDEVLRECGARDIPSKDDITKLKTLSMIVNESLRLYPPTVATIRQAKADV 420
Query: 421 ELGGYMIPRGTELLIPILAVHHDQKIWGNDVNEFNPGRFAEGVARAANHRVGFIPFGLGA 480
ELGGYMIPRGTELLIPILAVHHDQKIWGNDVNEFNPGRFAEGVARAANHRVGFIPFGLGA
Sbjct: 421 ELGGYMIPRGTELLIPILAVHHDQKIWGNDVNEFNPGRFAEGVARAANHRVGFIPFGLGA 480
Query: 481 RTCIGQNLAILQVKLALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKLSTPLI 540
RTCIGQNLAILQ KLALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKLSTP I
Sbjct: 481 RTCIGQNLAILQAKLALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKLSTPSI 521
Query: 541 HQD 544
QD
Sbjct: 541 QQD 521
BLAST of Spg003338 vs. ExPASy TrEMBL
Match:
A0A0A0LIW8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G006030 PE=3 SV=1)
HSP 1 Score: 957.6 bits (2474), Expect = 2.2e-275
Identity = 481/535 (89.91%), Postives = 497/535 (92.90%), Query Frame = 0
Query: 15 ILCCFLLLVALRAAVVLWWRPRKIEQHFFRQGIRGPPYRFFIGNVKELVGMMIKASSDHS 74
I C FL ++ LRA VVLWWRPR+IEQHFFRQGIRGPPYRFFIGNVKELVGMMIKASSDHS
Sbjct: 6 IFCWFLFVLVLRAVVVLWWRPRRIEQHFFRQGIRGPPYRFFIGNVKELVGMMIKASSDHS 65
Query: 75 FPHFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNE 134
FP+ SHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNE
Sbjct: 66 FPNSSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNE 125
Query: 135 PHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSAM-- 194
PHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSA+
Sbjct: 126 PHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSALMT 185
Query: 195 SAESGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAAQAFEKVFIPG 254
SA+S EVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLA+QAFEKVFIPG
Sbjct: 186 SADSDEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLASQAFEKVFIPG 245
Query: 255 YRYDDRHHYLNYYYYLTTTLRRFLPTRTNVNSWRLDKEIRKSLMKLIERRRENSIE-SSK 314
Y RFLPTRTNVNSWRLDKEIRKSLMKLI+RRRENSIE SSK
Sbjct: 246 Y--------------------RFLPTRTNVNSWRLDKEIRKSLMKLIDRRRENSIETSSK 305
Query: 315 DLLGLMIRASNSCPSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILMAMHPQWQVQAR 374
DLLGLMIRAS S PSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILMAMHPQWQVQAR
Sbjct: 306 DLLGLMIRASKSSPSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILMAMHPQWQVQAR 365
Query: 375 DEVLRECGARDIPSKDDVTKLKTLSMIVNESLRLYPPTVATIRQAKADVELGGYMIPRGT 434
DEVLRECGARDIPSKDDVTKLK LSMI+NESLRLYPPTVATIRQAK DVELGGYM+PRGT
Sbjct: 366 DEVLRECGARDIPSKDDVTKLKMLSMIINESLRLYPPTVATIRQAKVDVELGGYMLPRGT 425
Query: 435 ELLIPILAVHHDQKIWGNDVNEFNPGRFAEGVARAANHRVGFIPFGLGARTCIGQNLAIL 494
ELLIPILAVHHDQ IWGNDVNEFNP RFAEGVA+AANHRVGFIPFGLGARTCIGQNLAIL
Sbjct: 426 ELLIPILAVHHDQTIWGNDVNEFNPARFAEGVAKAANHRVGFIPFGLGARTCIGQNLAIL 485
Query: 495 QVKLALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKLSTPLIHQDQRS 547
Q KLALA+ILQRFSFRLGPSYQH+PAVQMLLYPR+GAPIIF+KLSTPL HQDQ+S
Sbjct: 486 QAKLALALILQRFSFRLGPSYQHSPAVQMLLYPRHGAPIIFKKLSTPLAHQDQQS 520
BLAST of Spg003338 vs. ExPASy TrEMBL
Match:
A0A6J1KGK2 (cytochrome P450 734A1-like OS=Cucurbita maxima OX=3661 GN=LOC111493691 PE=3 SV=1)
HSP 1 Score: 954.5 bits (2466), Expect = 1.9e-274
Identity = 474/533 (88.93%), Postives = 496/533 (93.06%), Query Frame = 0
Query: 15 ILCCFLLLVALRAAVVLWWRPRKIEQHFFRQGIRGPPYRFFIGNVKELVGMMIKASSDHS 74
ILC F+ L+ LRAAVVLWWRPRKIE HF RQGIRGPPYRFFIGNVKE+VGMMIKASS HS
Sbjct: 5 ILCSFMFLLVLRAAVVLWWRPRKIEHHFCRQGIRGPPYRFFIGNVKEIVGMMIKASSVHS 64
Query: 75 FPHFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNE 134
P+ SHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNE
Sbjct: 65 CPNSSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNE 124
Query: 135 PHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSAMSA 194
PHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVV+M+EKWSAMSA
Sbjct: 125 PHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVEMMEKWSAMSA 184
Query: 195 ESGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAAQAFEKVFIPGYR 254
+SGEVEIEVSEWFQTLTED+ITRTAFGSSYEDGKAIFRLQAQQMVLA+QAF+KV+IPGY
Sbjct: 185 DSGEVEIEVSEWFQTLTEDIITRTAFGSSYEDGKAIFRLQAQQMVLASQAFDKVYIPGY- 244
Query: 255 YDDRHHYLNYYYYLTTTLRRFLPTRTNVNSWRLDKEIRKSLMKLIERRRENSIE-SSKDL 314
RFLPTRTNVNSWRLD+EIRKSLMKLI+RR ENSIE SSKDL
Sbjct: 245 -------------------RFLPTRTNVNSWRLDREIRKSLMKLIDRRHENSIESSSKDL 304
Query: 315 LGLMIRASNSCPSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILMAMHPQWQVQARDE 374
LGLMIRAS S PSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILMAMHPQWQ+QARDE
Sbjct: 305 LGLMIRASKSSPSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILMAMHPQWQIQARDE 364
Query: 375 VLRECGARDIPSKDDVTKLKTLSMIVNESLRLYPPTVATIRQAKADVELGGYMIPRGTEL 434
VLR CGARD+PSKDDVTKLKTLSMI+NESLRLYPPTVATIRQAKADVE GGYMIPRGTEL
Sbjct: 365 VLRVCGARDVPSKDDVTKLKTLSMIINESLRLYPPTVATIRQAKADVEFGGYMIPRGTEL 424
Query: 435 LIPILAVHHDQKIWGNDVNEFNPGRFAEGVARAANHRVGFIPFGLGARTCIGQNLAILQV 494
LIPILAVHHDQ IWGNDVNEFNPGRFAEGVA+AANHRVGFIPFGLGARTCIGQNLAILQ
Sbjct: 425 LIPILAVHHDQTIWGNDVNEFNPGRFAEGVAKAANHRVGFIPFGLGARTCIGQNLAILQA 484
Query: 495 KLALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKLSTPLIHQDQRS 547
KLALA+ILQ+FSFRLGPSYQH+PAVQMLLYPRYGAPIIFQKLST ++HQDQRS
Sbjct: 485 KLALALILQKFSFRLGPSYQHSPAVQMLLYPRYGAPIIFQKLSTSMVHQDQRS 517
BLAST of Spg003338 vs. ExPASy TrEMBL
Match:
A0A6J1G3R6 (cytochrome P450 734A1-like OS=Cucurbita moschata OX=3662 GN=LOC111450496 PE=3 SV=1)
HSP 1 Score: 954.5 bits (2466), Expect = 1.9e-274
Identity = 475/533 (89.12%), Postives = 496/533 (93.06%), Query Frame = 0
Query: 15 ILCCFLLLVALRAAVVLWWRPRKIEQHFFRQGIRGPPYRFFIGNVKELVGMMIKASSDHS 74
ILC FL L+ LRAAVVLWWRPRKIE HF RQGIRGPPYRFFIGNVKE+VGMMIKASSDHS
Sbjct: 5 ILCSFLFLLGLRAAVVLWWRPRKIEHHFCRQGIRGPPYRFFIGNVKEIVGMMIKASSDHS 64
Query: 75 FPHFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNE 134
FP+ SHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNE
Sbjct: 65 FPNSSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNE 124
Query: 135 PHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSAMSA 194
PHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVV+MLEKWSAM A
Sbjct: 125 PHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVEMLEKWSAMLA 184
Query: 195 ESGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAAQAFEKVFIPGYR 254
+SGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLA+QAF+KV+IPGY
Sbjct: 185 DSGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLASQAFDKVYIPGY- 244
Query: 255 YDDRHHYLNYYYYLTTTLRRFLPTRTNVNSWRLDKEIRKSLMKLIERRRENSIE-SSKDL 314
RFLPTRTNVNSWRLD+EIRKSLMKLI+RR ENSIE SSKDL
Sbjct: 245 -------------------RFLPTRTNVNSWRLDREIRKSLMKLIDRRHENSIESSSKDL 304
Query: 315 LGLMIRASNSCPSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILMAMHPQWQVQARDE 374
LGLMIRAS S PSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMIL+AMHPQWQ+QARDE
Sbjct: 305 LGLMIRASKSSPSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILVAMHPQWQIQARDE 364
Query: 375 VLRECGARDIPSKDDVTKLKTLSMIVNESLRLYPPTVATIRQAKADVELGGYMIPRGTEL 434
VL CGARD+PSKDDVTKLKTLSMI+NESLRLYPPTVATIRQAKADVE GGYMIPRGTEL
Sbjct: 365 VLSVCGARDVPSKDDVTKLKTLSMIINESLRLYPPTVATIRQAKADVEFGGYMIPRGTEL 424
Query: 435 LIPILAVHHDQKIWGNDVNEFNPGRFAEGVARAANHRVGFIPFGLGARTCIGQNLAILQV 494
LIPILAVHHDQ IWGNDVNEFNPGRFAEGVA+AANHRVGFIPFGLGARTCIGQNLAILQ
Sbjct: 425 LIPILAVHHDQTIWGNDVNEFNPGRFAEGVAKAANHRVGFIPFGLGARTCIGQNLAILQA 484
Query: 495 KLALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKLSTPLIHQDQRS 547
KLALA+ILQ+FSFRLGPSYQH+PAVQMLLYPRYGAPIIF+KLST L+HQDQ+S
Sbjct: 485 KLALALILQKFSFRLGPSYQHSPAVQMLLYPRYGAPIIFEKLSTSLLHQDQQS 517
BLAST of Spg003338 vs. TAIR 10
Match:
AT2G26710.1 (Cytochrome P450 superfamily protein )
HSP 1 Score: 765.8 bits (1976), Expect = 2.4e-221
Identity = 382/541 (70.61%), Postives = 444/541 (82.07%), Query Frame = 0
Query: 12 RVSILCCFLLLVALRAAVVLWWRPRKIEQHFFRQGIRGPPYRFFIGNVKELVGMMIKASS 71
+V +L L LV ++ +LWWRPRKIE+HF +QGIRGPPY FFIGNVKELVGMM+KASS
Sbjct: 12 KVLVLSVILSLVIVKGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKASS 71
Query: 72 DHSFPHFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCE 131
H P FSHNILPRVL FYHHW+KIYG+ FLVWFGPT RL V+DPDLIREIF SKSEF E
Sbjct: 72 -HPMP-FSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIF-SKSEFYE 131
Query: 132 KNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSA 191
KNE HPLVKQLEGDGLLSLKG+KWA HRKIISP+FHM+NLKLL+PV+ KSV DM++KWS
Sbjct: 132 KNEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSD 191
Query: 192 MSAESGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAAQAFEKVFIP 251
+E+GEVE++V EWFQ LTEDVI+RTAFGSSYEDG+A+FRLQAQQM+L A+AF+KVFIP
Sbjct: 192 KLSENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFIP 251
Query: 252 GYRYDDRHHYLNYYYYLTTTLRRFLPTRTNVNSWRLDKEIRKSLMKLIERRRENSIE--- 311
GY RF PTR N+ SW+LDKEIRKSL+KLIERRR+N+I+
Sbjct: 252 GY--------------------RFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEG 311
Query: 312 ------SSKDLLGLMIRASNSCPSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILMAM 371
++KDLLGLMI+A N +TV DIVEECK FFFAGKQTTSNLLTWT IL++M
Sbjct: 312 EECKEPAAKDLLGLMIQAKN------VTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSM 371
Query: 372 HPQWQVQARDEVLRECGARDIPSKDDVTKLKTLSMIVNESLRLYPPTVATIRQAKADVEL 431
HP+WQ +ARDEVLR CG+RD+P+KD V KLKTLSMI+NESLRLYPP VATIR+AK+DV+L
Sbjct: 372 HPEWQAKARDEVLRVCGSRDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKL 431
Query: 432 GGYMIPRGTELLIPILAVHHDQKIWGNDVNEFNPGRFAEGVARAANHRVGFIPFGLGART 491
GGY IP GTELLIPI+AVHHDQ IWGNDVNEFNP RFA+GV RAA H VGFIPFGLG RT
Sbjct: 432 GGYKIPCGTELLIPIIAVHHDQAIWGNDVNEFNPARFADGVPRAAKHPVGFIPFGLGVRT 491
Query: 492 CIGQNLAILQVKLALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKLSTPLIHQ 544
CIGQNLAILQ KL LA+++QRF+F L P+YQHAP V MLLYP++GAPI F++L+ H+
Sbjct: 492 CIGQNLAILQAKLTLAVMIQRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRLTN---HE 520
BLAST of Spg003338 vs. TAIR 10
Match:
AT3G14620.1 (cytochrome P450, family 72, subfamily A, polypeptide 8 )
HSP 1 Score: 397.5 bits (1020), Expect = 1.7e-110
Identity = 206/512 (40.23%), Postives = 318/512 (62.11%), Query Frame = 0
Query: 30 VLWWRPRKIEQHFFRQGIRGPPYRFFIGNVKELVGMMIKASSDHSFP-HFSHNILPRVLP 89
V W RP+K E + RQG+ G P+ F +G++K M+ + S P + + + RV+P
Sbjct: 31 VAWLRPKKNEAYLKRQGLSGTPFTFLVGDIKREASMV---EQEKSRPINLTDDYTHRVMP 90
Query: 90 FYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNEPHPLVKQLEGDGLL 149
K +G +W GP + V+ P+ I+++ +F K HP+V +L G+
Sbjct: 91 LIQQTVKDHGKTSYMWMGPIASVIVTKPEHIKDVLNRVYDF-PKPPVHPIV-ELFATGVA 150
Query: 150 SLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSAMSAESGEV-EIEVSEWF 209
+G+KW+ HRKII+PSFH++ LK++IP +S +M+ KW + E G EI+V +
Sbjct: 151 LYEGEKWSKHRKIINPSFHLEKLKIMIPAFYESCSEMISKWEKLVTEQGSSNEIDVWPYL 210
Query: 210 QTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAAQAFEKVFIPGYRYDDRHHYLNYYYY 269
LT DVI+RTAFGSSYE+GK IF LQ +Q +A E FIPG
Sbjct: 211 GDLTSDVISRTAFGSSYEEGKRIFELQEEQGRRVLKALELAFIPG--------------- 270
Query: 270 LTTTLRRFLPTRTNVNSWRLDKEIRKSLMKLIERRR---ENSIESSKDLLGLMIRASNSC 329
RFLPT+ N+ +++KE++ L ++I +R+ + DLLG+++ +++
Sbjct: 271 -----MRFLPTKNNLRMRQINKEVKSRLREIIMKRQRGMDTGEAPKNDLLGILLESNSG- 330
Query: 330 PSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILMAMHPQWQVQARDEVLRECGARDIP 389
+++ D+VEEC+ F FAG++TT+ LL WTMI+++ H +WQ QAR+E+L+ G + P
Sbjct: 331 -DHGMSIEDVVEECRLFHFAGQETTAVLLVWTMIMLSHHQKWQDQAREEILKVIGKNNKP 390
Query: 390 SKDDVTKLKTLSMIVNESLRLYPPTVATIRQAKADVELGGYM-IPRGTELLIPILAVHHD 449
+ D +++LKT+SMI+NE LRLYPP + R + + +LG M +P G +++IP+L VH D
Sbjct: 391 NFDALSRLKTMSMILNEVLRLYPPGILLGRTVEKETKLGEDMTLPGGAQVVIPVLMVHRD 450
Query: 450 QKIWGNDVNEFNPGRFAEGVARAANHRVGFIPFGLGARTCIGQNLAILQVKLALAIILQR 509
++WG DV+EFNP RFA+G+++A ++V F+PFG G R C GQN A+++ K+AL +ILQR
Sbjct: 451 PELWGEDVHEFNPERFADGISKATKNQVSFLPFGWGPRFCPGQNFALMEAKMALVLILQR 510
Query: 510 FSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKL 536
FSF L PSY HAP + L+P++GAP+IF L
Sbjct: 511 FSFELSPSYTHAPHTVLTLHPQFGAPLIFHML 515
BLAST of Spg003338 vs. TAIR 10
Match:
AT2G46950.1 (cytochrome P450, family 709, subfamily B, polypeptide 2 )
HSP 1 Score: 391.0 bits (1003), Expect = 1.6e-108
Identity = 202/530 (38.11%), Postives = 313/530 (59.06%), Query Frame = 0
Query: 13 VSILCCFLLLVAL----RAAVVLWWRPRKIEQHFFRQGIRGPPYRFFIGNVKELVGMMIK 72
+++L L+L+ + A +L WRP + + F +QGI GP YR GN++E+ M +
Sbjct: 62 INLLAIALVLLVVPKIYGACRILVWRPWMLSRRFKKQGISGPKYRILYGNLREIRKMKNE 121
Query: 73 ASSDHSFPHFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSE 132
A P+ S++I+PRVLP WK YG FL W G RL +SD +L ++I ++K
Sbjct: 122 AKLMVLDPN-SNDIVPRVLPHLQQWKSQYGETFLYWQGTDPRLCISDHELAKQILSNKFV 181
Query: 133 FCEKNEPHPLVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEK 192
F K++ P + +L G+GL+ + G W HR+I++P+F MD LKL+ +M M +
Sbjct: 182 FFSKSKTKPEILKLSGNGLIFVNGLDWVRHRRILNPAFSMDKLKLMTQLMVDCTFRMFLE 241
Query: 193 WSAM-SAESGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAAQAFEK 252
W + E + +S F+ LT D+I AFGSSY +G +F+ Q + A A
Sbjct: 242 WKKQRNGVETEQFVLISREFKRLTADIIATAAFGSSYAEGIEVFKSQLELQKCCAAALTD 301
Query: 253 VFIPGYRYDDRHHYLNYYYYLTTTLRRFLPTRTNVNSWRLDKEIRKSLMKLIERR-RENS 312
++ PG +Y LPT +N+ W+LD ++ S+ ++I+ R S
Sbjct: 302 LYFPGIQY--------------------LPTPSNLQIWKLDMKVNSSIKRIIDARLTSES 361
Query: 313 IESSKDLLGLMIRASNSCPS-STITVNDIVEECKGFFFAGKQTTSNLLTWTMILMAMHPQ 372
+ DLLG+M+ A++S S +++++I+EECK FFFAG +TT+NLLTW+ +L+++H
Sbjct: 362 KDYGNDLLGIMLTAASSNESEKKMSIDEIIEECKTFFFAGHETTANLLTWSTMLLSLHQD 421
Query: 373 WQVQARDEVLRECGARDIPSKDDVTKLKTLSMIVNESLRLYPPTVATIRQAKADVELGGY 432
WQ + R+EV ECG IP + +KLK ++ + ESLRLY P + +R A D++LG
Sbjct: 422 WQEKLREEVFNECGKDKIPDAETCSKLKLMNTVFMESLRLYGPVLNLLRLASEDMKLGNL 481
Query: 433 MIPRGTELLIPILAVHHDQKIWGNDVNEFNPGRFAEGVARAANHRVGFIPFGLGARTCIG 492
IP+GT +++PI +H D+ +WG+D ++FNP RFA G++RAANH + F +G R CIG
Sbjct: 482 EIPKGTTIILPIAKMHRDKAVWGSDADKFNPMRFANGLSRAANHPNALLAFSMGPRACIG 541
Query: 493 QNLAILQVKLALAIILQRFSFRLGPSYQHAPAVQMLLYPRYGAPIIFQKL 536
QN AI++ K LA+ILQRF L Y+HAPA + L P+Y P+I + +
Sbjct: 542 QNFAIMEAKTVLAMILQRFRLNLSADYKHAPADHLTLQPQYDLPVILEPI 570
BLAST of Spg003338 vs. TAIR 10
Match:
AT1G75130.1 (cytochrome P450, family 721, subfamily A, polypeptide 1 )
HSP 1 Score: 384.8 bits (987), Expect = 1.2e-106
Identity = 202/508 (39.76%), Postives = 301/508 (59.25%), Query Frame = 0
Query: 33 WRPRKIEQHFFRQGIRGPPYRFFIGNVKELVGMMIKASSDH-SFPHFSHNILPRVLPFYH 92
W P +I+ HF +Q + GP YR F GN E+ + +A S H + RV P YH
Sbjct: 24 WVPWRIQSHFKKQSVTGPSYRIFSGNSGEVSRLTAEAKSKPIPSGRNPHEFVHRVAPHYH 83
Query: 93 HWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEFCEKNEPHPLVKQLEGDGLLSLK 152
W ++YG FL WFG +A SDP LIRE T+ F ++ +PL K L GL L+
Sbjct: 84 EWSRVYGKTFLYWFGSKPVVATSDPRLIREALTTGGSF-DRIGHNPLSKLLYAQGLPGLR 143
Query: 153 GQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEKWSAMSAESGEVEIEVSEWFQTLT 212
G +WA HR+I +F M+ LK +P M S + ++EKW M E+E+EV + L+
Sbjct: 144 GDQWAFHRRIAKQAFTMEKLKRWVPQMVTSTMMLMEKWEDMRNGGEEIELEVHKEMHNLS 203
Query: 213 EDVITRTAFGSSYEDGKAIFRLQAQQMVLAAQAFEKVFIPGYRYDDRHHYLNYYYYLTTT 272
++++RTAFG+S E+GK IF LQ + M L V+IPG+
Sbjct: 204 AEMLSRTAFGNSVEEGKGIFELQERMMRLFYLVRWSVYIPGF------------------ 263
Query: 273 LRRFLPTRTNVNSWRLDKEIRKSLMKLIERRRENSIESSKDLLGLMIR--ASNSCPSSTI 332
RF P++TN WR++K+IR S++KLIE + ++E S LL + + + +
Sbjct: 264 --RFFPSKTNREIWRIEKQIRVSILKLIENNK-TAVEKSGTLLQAFMSPYTNQNGQEEKL 323
Query: 333 TVNDIVEECKGFFFAGKQTTSNLLTWTMILMAMHPQWQVQARDEVLRECGARDIPSKDDV 392
+ ++ +ECK F+FA K+TT+NL+T+ ++L+AM+ +WQ AR+EV+ G +P+ D +
Sbjct: 324 GIEEVTDECKTFYFAAKETTANLMTFVLVLLAMNQEWQNIAREEVICVLGQTGLPTLDIL 383
Query: 393 TKLKTLSMIVNESLRLYPPTVATIRQAKADVELGGYMIPRGTELLIPILAVHHDQKIWGN 452
LKTLSMI+NE+LRLYPP + R +LG IP GT+L + ++A+HHD++ WG+
Sbjct: 384 QDLKTLSMIINETLRLYPPAMTLNRDTLKRAKLGDLDIPAGTQLYLSVVAMHHDKETWGD 443
Query: 453 DVNEFNPGRFAEGVARAANHRVGFIPFGLGARTCIGQNLAILQVKLALAIILQRFSFRLG 512
D EFNP RF + ++A +PFGLG RTC+GQNLA+ + K LA IL+ +SFRL
Sbjct: 444 DAEEFNPRRFEDPKKQSAL----LVPFGLGPRTCVGQNLAVNEAKTVLATILKYYSFRLS 503
Query: 513 PSYQHAPAVQMLLYPRYGAPIIFQKLST 538
PSY HAP + + L P+ GA ++F ++S+
Sbjct: 504 PSYAHAPVLFVTLQPQNGAHLLFTRISS 505
BLAST of Spg003338 vs. TAIR 10
Match:
AT2G46960.2 (cytochrome P450, family 709, subfamily B, polypeptide 1 )
HSP 1 Score: 382.1 bits (980), Expect = 7.5e-106
Identity = 195/533 (36.59%), Postives = 312/533 (58.54%), Query Frame = 0
Query: 10 WLRVSILCCFLLLVALRAAVVLWWRPRKIEQHFFRQGIRGPPYRFFIGNVKELVGMMIKA 69
+L + +L + L +A ++L W P + + QGI GP YR F GN+ E+ M ++
Sbjct: 6 FLALIVLILIIGLRIFKAFMILVWHPFVLTRRLKNQGISGPNYRIFYGNLSEIKKMKRES 65
Query: 70 SSDHSFPHFSHNILPRVLPFYHHWKKIYGSKFLVWFGPTVRLAVSDPDLIREIFTSKSEF 129
P S++I PR+LP Y W YG FL W G R+ +SDP+L + + ++K F
Sbjct: 66 HLSILDPS-SNDIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPELAKTMLSNKLGF 125
Query: 130 CEKNEPHP-LVKQLEGDGLLSLKGQKWALHRKIISPSFHMDNLKLLIPVMAKSVVDMLEK 189
K++ P VK + GL+ ++G W HR+I++P+F +D LK++ VM + MLE+
Sbjct: 126 FVKSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLKIMTTVMVDCTLKMLEE 185
Query: 190 WSAMS----AESGEVEIEVSEWFQTLTEDVITRTAFGSSYEDGKAIFRLQAQQMVLAAQA 249
W S E +++ E++E FQ LT D+I +AFGSSY +G +FR Q + +
Sbjct: 186 WRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFRSQMELKRCYTTS 245
Query: 250 FEKVFIPGYRYDDRHHYLNYYYYLTTTLRRFLPTRTNVNSWRLDKEIRKSLMKLIERRRE 309
+V IPG +Y LPT +N+ W+L++++ S+ ++I R +
Sbjct: 246 LNQVSIPGTQY--------------------LPTPSNIRVWKLERKMDNSIKRIISSRLQ 305
Query: 310 NSIESSKDLLGLMIRASNS-CPSSTITVNDIVEECKGFFFAGKQTTSNLLTWTMILMAMH 369
+ + DLLG++++A N+ +++ +I+ EC+ FFF G +TTSNLL WT +L+++H
Sbjct: 306 SKSDYGDDLLGILLKAYNTEGKERKMSIEEIIHECRTFFFGGHETTSNLLAWTTMLLSLH 365
Query: 370 PQWQVQARDEVLRECGARDIPSKDDVTKLKTLSMIVNESLRLYPPTVATIRQAKADVELG 429
WQ + R+E+ +ECG P + +KLK ++M++ ESLRLY P A R+A +++LG
Sbjct: 366 QDWQEKLREEIFKECGKEKTPDSETFSKLKLMNMVIMESLRLYGPVSALAREASVNIKLG 425
Query: 430 GYMIPRGTELLIPILAVHHDQKIWGNDVNEFNPGRFAEGVARAANHRVGFIPFGLGARTC 489
IP+GT ++IP+L +H D+ +WG+D ++FNP RFA GV+RAANH + F +G R C
Sbjct: 426 DLEIPKGTTVVIPLLKMHSDKTLWGSDADKFNPMRFANGVSRAANHPNALLAFSVGPRAC 485
Query: 490 IGQNLAILQVKLALAIILQRFSF-RLGPSYQHAPAVQMLLYPRYGAPIIFQKL 536
IGQN +++ K L +ILQRF F L Y+H P + + P+YG P++ Q L
Sbjct: 486 IGQNFVMIEAKTVLTMILQRFRFISLCDEYKHTPVDNVTIQPQYGLPVMLQPL 517
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| O48786 | 3.4e-220 | 70.61 | Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1 | [more] |
| B9X287 | 2.2e-187 | 60.15 | Cytochrome P450 734A6 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A6 PE=2 ... | [more] |
| Q6Z6D6 | 1.2e-172 | 54.93 | Cytochrome P450 734A2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A2 PE=2 ... | [more] |
| Q69XM6 | 1.5e-164 | 52.45 | Cytochrome P450 734A4 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A4 PE=2 ... | [more] |
| Q8LIF2 | 2.2e-147 | 49.14 | Cytochrome P450 734A5 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A5 PE=2 ... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1HMB5 | 3.3e-287 | 91.58 | cytochrome P450 734A1-like OS=Cucurbita maxima OX=3661 GN=LOC111465504 PE=3 SV=1 | [more] |
| A0A6J1FI14 | 2.2e-286 | 91.71 | cytochrome P450 734A1-like OS=Cucurbita moschata OX=3662 GN=LOC111444133 PE=3 SV... | [more] |
| A0A0A0LIW8 | 2.2e-275 | 89.91 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G006030 PE=3 SV=1 | [more] |
| A0A6J1KGK2 | 1.9e-274 | 88.93 | cytochrome P450 734A1-like OS=Cucurbita maxima OX=3661 GN=LOC111493691 PE=3 SV=1 | [more] |
| A0A6J1G3R6 | 1.9e-274 | 89.12 | cytochrome P450 734A1-like OS=Cucurbita moschata OX=3662 GN=LOC111450496 PE=3 SV... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT2G26710.1 | 2.4e-221 | 70.61 | Cytochrome P450 superfamily protein | [more] |
| AT3G14620.1 | 1.7e-110 | 40.23 | cytochrome P450, family 72, subfamily A, polypeptide 8 | [more] |
| AT2G46950.1 | 1.6e-108 | 38.11 | cytochrome P450, family 709, subfamily B, polypeptide 2 | [more] |
| AT1G75130.1 | 1.2e-106 | 39.76 | cytochrome P450, family 721, subfamily A, polypeptide 1 | [more] |
| AT2G46960.2 | 7.5e-106 | 36.59 | cytochrome P450, family 709, subfamily B, polypeptide 1 | [more] |
