Spg003255 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg003255
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	phospholipase A(1) LCAT3
Location	scaffold4: 31426419 .. 31428054 (+)
RNA-Seq Expression	Spg003255
Synteny	Spg003255

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAGTTTAAAGGTTAAAAATGCTTTTTTCCCTTTTTTTTATTAAACCAATTTTTTTAATTTCCCAAACATAATGGTCAAAATGCATTAAATACGTAGACTACCTGCCAATCAACCACGAGTACCTTTCTTACATCCCAACAACAAAAAAAAAAAAAAAAAGAAGAAAAAAAAGAAAACGAAAAAGACGAAAAGAAAACCACGTAGCAGTGAAGACCTTTCAAAATCAGTTTCTTCTTCCAGTTTCAGCTTTCTTCAATCCCTCTCCATCGCAGAGCTTCCAGTAAGCAGCATCGAGAACAGAGGCTCCTCCATTTCACCCTGTATTTCAACTGAGCTTAAAGAAGCTCAGTTCATTTCATTTTCAAGAGCCATGGTCGGATGCTTTCTTCCTCTCCCATGTTTCAGGACCCGGAAGCCCGAACCCGAACCCGACCGCGACCCGGTACTACTCCTCTCCGGCATTGGAGGCTCCATTATTAACGCCAAGAACAAGAAATTGTTCGGACTACAGACCCGAGTATGGGTTCGGATCTTCTTGTCTGACCTGGTGTTTCGTGAGAAGCTCGTTTCACTCTACAATCCCGACACAGGTCCTTCACTCTTTTTAATTCAAATTTCCTCTCTGTTTGTTTGTTGCCTTTTGTTTTTGGTTGCCATGGTAGTGACGGAATCTAGATGCTTTTAGATTATTGCGAGTGCACGTGTTTTGGGGTTCTTTTTAGGCTGGGATGAAATTTCGGAGCACTTTATTCTTCCTTTAGAAAGGGAAAGTGTTTTTAATGTGTTTTGGACTTTTGGTTACATTATTTTAAAACTGCTTTTGATCATATTTTTTTTTTGTTGCACTGTGTCAAAAAATATTCTACTAATCAGAAACTTTTTTTAATTTTACATATATATAATTTTCATTTTCTTAAAAGTTCTTTTAACCATTTTTTTTTGTGATTTTATTAATCACAATTTTTTTCCCTCATGATTTCTGGTAAACACTTTTGTGGAGAAGAGTCTCTAAAATGTTTCTTAAGAAGTGTTTTTATTTATTTATTTATCCATTTATTATTAATTTTTAATATTATTTTTTTGTAAACCCTGTTGATTAAAAAAAAAAAAACAATTTTAGCTGCCTAAAAAACTACTTAGGATTTAGGACACTTTGTCAAACACACTTTATAAATAAGTTTGATGTTCTTCTTAAGTGCCATTCTGTTTACCAAGTCAATCTGCCGGTAAGAGAATGATAGGAACCATATAATAAGACTTGGAAATTATATTGAAATATCAATAAGAGATTACTACAAAATACTTATTTTGAAGTACCTGAAACCTCCCCCTTGTGAACTCTCAAGACTAAACCACTGCAATAACGTCTACCTCCCTCCGGAGCCCAACCCTTCTATTCATAACAAATTCCTAAGACGCTACCTTGCCGATGACTTTATTATTATGGCCATTGAGTATAAAGGGACAAGTGGGATTTTTTTAGTTCTGTTGATGATCAAATTTCGCTAGGTAAGTTACCTTTGCTCCACGTGATAATCGACAGTGAATTTATTTGAGAGCGGGGGAAGACGTGGCCTGCAAAAGAAAAAACCTGCACACCGGTGTGGTGCTCACCACACCGCCTCCGATGCTTAA

mRNA sequence

ATGGTCGGATGCTTTCTTCCTCTCCCATGTTTCAGGACCCGGAAGCCCGAACCCGAACCCGACCGCGACCCGGTACTACTCCTCTCCGGCATTGGAGGCTCCATTATTAACGCCAAGAACAAGAAATTGTTCGGACTACAGACCCGAGTATGGGTTCGGATCTTCTTGTCTGACCTGGTGTTTCGTGAGAAGCTCGTTTCACTCTACAATCCCGACACAGTGAATTTATTTGAGAGCGGGGGAAGACGTGGCCTGCAAAAGAAAAAACCTGCACACCGGTGTGGTGCTCACCACACCGCCTCCGATGCTTAA

Coding sequence (CDS)

ATGGTCGGATGCTTTCTTCCTCTCCCATGTTTCAGGACCCGGAAGCCCGAACCCGAACCCGACCGCGACCCGGTACTACTCCTCTCCGGCATTGGAGGCTCCATTATTAACGCCAAGAACAAGAAATTGTTCGGACTACAGACCCGAGTATGGGTTCGGATCTTCTTGTCTGACCTGGTGTTTCGTGAGAAGCTCGTTTCACTCTACAATCCCGACACAGTGAATTTATTTGAGAGCGGGGGAAGACGTGGCCTGCAAAAGAAAAAACCTGCACACCGGTGTGGTGCTCACCACACCGCCTCCGATGCTTAA

Protein sequence

MVGCFLPLPCFRTRKPEPEPDRDPVLLLSGIGGSIINAKNKKLFGLQTRVWVRIFLSDLVFREKLVSLYNPDTVNLFESGGRRGLQKKKPAHRCGAHHTASDA

Homology

[Close]

BLAST of Spg003255 vs. NCBI nr
Match: KAA0051379.1 (phospholipase A(1) LCAT3 [Cucumis melo var. makuwa] >TYK23844.1 phospholipase A(1) LCAT3 [Cucumis melo var. makuwa])

HSP 1 Score: 126.7 bits (317), Expect = 1.1e-25
Identity = 57/71 (80.28%), Postives = 65/71 (91.55%), Query Frame = 0


Query: 3  GCFLPLPCFRTRKPEPEPDRDPVLLLSGIGGSIINAKNKKLFGLQTRVWVRIFLSDLVFR 62
          GCFL LP F +RK EPEPDRDPVLL+SGIGGSI+++KNKKLFGLQTRVWVRIFLSD+VFR
Sbjct: 6  GCFLSLPFFGSRKSEPEPDRDPVLLVSGIGGSILHSKNKKLFGLQTRVWVRIFLSDIVFR 65

Query: 63 EKLVSLYNPDT 74
          E L+S+YNP T
Sbjct: 66 ENLISIYNPHT 76

[Close]

BLAST of Spg003255 vs. NCBI nr
Match: XP_008441766.1 (PREDICTED: phospholipase A(1) LCAT3 [Cucumis melo])

HSP 1 Score: 126.7 bits (317), Expect = 1.1e-25
Identity = 57/71 (80.28%), Postives = 65/71 (91.55%), Query Frame = 0


Query: 3  GCFLPLPCFRTRKPEPEPDRDPVLLLSGIGGSIINAKNKKLFGLQTRVWVRIFLSDLVFR 62
          GCFL LP F +RK EPEPDRDPVLL+SGIGGSI+++KNKKLFGLQTRVWVRIFLSD+VFR
Sbjct: 6  GCFLSLPFFGSRKSEPEPDRDPVLLVSGIGGSILHSKNKKLFGLQTRVWVRIFLSDIVFR 65

Query: 63 EKLVSLYNPDT 74
          E L+S+YNP T
Sbjct: 66 ENLISIYNPHT 76

[Close]

BLAST of Spg003255 vs. NCBI nr
Match: XP_022144495.1 (phospholipase A(1) LCAT3 [Momordica charantia])

HSP 1 Score: 125.2 bits (313), Expect = 3.3e-25
Identity = 54/71 (76.06%), Postives = 66/71 (92.96%), Query Frame = 0


Query: 3  GCFLPLPCFRTRKPEPEPDRDPVLLLSGIGGSIINAKNKKLFGLQTRVWVRIFLSDLVFR 62
          G  LPLPCFRTRKP+PEPDRDPVLL+SGIGGSI++++NKKLFGL+TRVWVRI L++L+F 
Sbjct: 4  GGLLPLPCFRTRKPQPEPDRDPVLLVSGIGGSILHSRNKKLFGLETRVWVRIILAELLFN 63

Query: 63 EKLVSLYNPDT 74
          E+L+SLYNP T
Sbjct: 64 ERLISLYNPKT 74

[Close]

BLAST of Spg003255 vs. NCBI nr
Match: XP_022973913.1 (phospholipase A(1) LCAT3 [Cucurbita maxima])

HSP 1 Score: 122.5 bits (306), Expect = 2.2e-24
Identity = 58/68 (85.29%), Postives = 63/68 (92.65%), Query Frame = 0


Query: 8  LPCFRTRKP--EPEPDRDPVLLLSGIGGSIINAKNKKLFGLQTRVWVRIFLSDLVFREKL 67
          LPCF +R P  EPEPDRDPVLL+SGIGGSII+AKNKKLFGLQTRVWVRIFLSDLVFRE L
Sbjct: 13 LPCFGSRMPESEPEPDRDPVLLVSGIGGSIIHAKNKKLFGLQTRVWVRIFLSDLVFRENL 72

Query: 68 VSLYNPDT 74
          +S+YNPDT
Sbjct: 73 ISIYNPDT 80

[Close]

BLAST of Spg003255 vs. NCBI nr
Match: XP_004138893.1 (phospholipase A(1) LCAT3 [Cucumis sativus])

HSP 1 Score: 122.5 bits (306), Expect = 2.2e-24
Identity = 54/71 (76.06%), Postives = 64/71 (90.14%), Query Frame = 0


Query: 3  GCFLPLPCFRTRKPEPEPDRDPVLLLSGIGGSIINAKNKKLFGLQTRVWVRIFLSDLVFR 62
          G  L LPCF +RK EPEPDRDPVLL+SGIGGSI+++KNKKLFGLQTRVWVR+FLSD+VFR
Sbjct: 5  GGLLSLPCFGSRKSEPEPDRDPVLLVSGIGGSILHSKNKKLFGLQTRVWVRVFLSDIVFR 64

Query: 63 EKLVSLYNPDT 74
          + L+S+YNP T
Sbjct: 65 QNLISIYNPHT 75

[Close]

BLAST of Spg003255 vs. ExPASy Swiss-Prot
Match: Q93V61 (Phospholipase A(1) LCAT3 OS=Arabidopsis thaliana OX=3702 GN=LCAT3 PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 5.2e-13
Identity = 39/72 (54.17%), Postives = 51/72 (70.83%), Query Frame = 0


Query: 5  FLPLPCFRTRKPE---PEPDRDPVLLLSGIGGSIINAKNKKLFGLQTRVWVRIFLSDLVF 64
          ++P PC+ T   E      DRDPVLL+SGIGGSI+++K KK    + RVWVRIFL++L F
Sbjct: 3  WIPCPCWGTNDDENAGEVADRDPVLLVSGIGGSILHSK-KKNSKSEIRVWVRIFLANLAF 62

Query: 65 REKLVSLYNPDT 74
          ++ L SLYNP T
Sbjct: 63 KQSLWSLYNPKT 73

[Close]

BLAST of Spg003255 vs. ExPASy Swiss-Prot
Match: Q71N54 (Lecithin-cholesterol acyltransferase-like 4 OS=Arabidopsis thaliana OX=3702 GN=LCAT4 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 6.6e-08
Identity = 29/62 (46.77%), Postives = 43/62 (69.35%), Query Frame = 0


Query: 12 RTRKPEPEPDRDPVLLLSGIGGSIINAKNKKLFGLQTRVWVRIFLSDLVFREKLVSLYNP 71
          R ++P  +P+ +PVLL+ GI GSI+NA + +  G + RVWVRIF +D  FR K+ S ++P
Sbjct: 21 RNQEPYVDPNLNPVLLVPGIAGSILNAVDHE-NGNEERVWVRIFGADHEFRTKMWSRFDP 80

Query: 72 DT 74
           T
Sbjct: 81 ST 81

[Close]

BLAST of Spg003255 vs. ExPASy TrEMBL
Match: A0A5D3DJN1 (Phospholipase A(1) LCAT3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold419G00080 PE=4 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 5.5e-26
Identity = 57/71 (80.28%), Postives = 65/71 (91.55%), Query Frame = 0


Query: 3  GCFLPLPCFRTRKPEPEPDRDPVLLLSGIGGSIINAKNKKLFGLQTRVWVRIFLSDLVFR 62
          GCFL LP F +RK EPEPDRDPVLL+SGIGGSI+++KNKKLFGLQTRVWVRIFLSD+VFR
Sbjct: 6  GCFLSLPFFGSRKSEPEPDRDPVLLVSGIGGSILHSKNKKLFGLQTRVWVRIFLSDIVFR 65

Query: 63 EKLVSLYNPDT 74
          E L+S+YNP T
Sbjct: 66 ENLISIYNPHT 76

[Close]

BLAST of Spg003255 vs. ExPASy TrEMBL
Match: A0A1S3B3Q4 (phospholipase A(1) LCAT3 OS=Cucumis melo OX=3656 GN=LOC103485824 PE=4 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 5.5e-26
Identity = 57/71 (80.28%), Postives = 65/71 (91.55%), Query Frame = 0


Query: 3  GCFLPLPCFRTRKPEPEPDRDPVLLLSGIGGSIINAKNKKLFGLQTRVWVRIFLSDLVFR 62
          GCFL LP F +RK EPEPDRDPVLL+SGIGGSI+++KNKKLFGLQTRVWVRIFLSD+VFR
Sbjct: 6  GCFLSLPFFGSRKSEPEPDRDPVLLVSGIGGSILHSKNKKLFGLQTRVWVRIFLSDIVFR 65

Query: 63 EKLVSLYNPDT 74
          E L+S+YNP T
Sbjct: 66 ENLISIYNPHT 76

[Close]

BLAST of Spg003255 vs. ExPASy TrEMBL
Match: A0A6J1CRS5 (phospholipase A(1) LCAT3 OS=Momordica charantia OX=3673 GN=LOC111014171 PE=4 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 1.6e-25
Identity = 54/71 (76.06%), Postives = 66/71 (92.96%), Query Frame = 0


Query: 3  GCFLPLPCFRTRKPEPEPDRDPVLLLSGIGGSIINAKNKKLFGLQTRVWVRIFLSDLVFR 62
          G  LPLPCFRTRKP+PEPDRDPVLL+SGIGGSI++++NKKLFGL+TRVWVRI L++L+F 
Sbjct: 4  GGLLPLPCFRTRKPQPEPDRDPVLLVSGIGGSILHSRNKKLFGLETRVWVRIILAELLFN 63

Query: 63 EKLVSLYNPDT 74
          E+L+SLYNP T
Sbjct: 64 ERLISLYNPKT 74

[Close]

BLAST of Spg003255 vs. ExPASy TrEMBL
Match: A0A6J1IEK6 (phospholipase A(1) LCAT3 OS=Cucurbita maxima OX=3661 GN=LOC111472543 PE=4 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 1.0e-24
Identity = 58/68 (85.29%), Postives = 63/68 (92.65%), Query Frame = 0


Query: 8  LPCFRTRKP--EPEPDRDPVLLLSGIGGSIINAKNKKLFGLQTRVWVRIFLSDLVFREKL 67
          LPCF +R P  EPEPDRDPVLL+SGIGGSII+AKNKKLFGLQTRVWVRIFLSDLVFRE L
Sbjct: 13 LPCFGSRMPESEPEPDRDPVLLVSGIGGSIIHAKNKKLFGLQTRVWVRIFLSDLVFRENL 72

Query: 68 VSLYNPDT 74
          +S+YNPDT
Sbjct: 73 ISIYNPDT 80

[Close]

BLAST of Spg003255 vs. ExPASy TrEMBL
Match: A0A0A0LHR5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G093800 PE=4 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 1.0e-24
Identity = 54/71 (76.06%), Postives = 64/71 (90.14%), Query Frame = 0


Query: 3  GCFLPLPCFRTRKPEPEPDRDPVLLLSGIGGSIINAKNKKLFGLQTRVWVRIFLSDLVFR 62
          G  L LPCF +RK EPEPDRDPVLL+SGIGGSI+++KNKKLFGLQTRVWVR+FLSD+VFR
Sbjct: 5  GGLLSLPCFGSRKSEPEPDRDPVLLVSGIGGSILHSKNKKLFGLQTRVWVRVFLSDIVFR 64

Query: 63 EKLVSLYNPDT 74
          + L+S+YNP T
Sbjct: 65 QNLISIYNPHT 75

[Close]

BLAST of Spg003255 vs. TAIR 10
Match: AT3G03310.1 (lecithin:cholesterol acyltransferase 3 )

HSP 1 Score: 75.1 bits (183), Expect = 3.7e-14
Identity = 39/72 (54.17%), Postives = 51/72 (70.83%), Query Frame = 0


Query: 5  FLPLPCFRTRKPE---PEPDRDPVLLLSGIGGSIINAKNKKLFGLQTRVWVRIFLSDLVF 64
          ++P PC+ T   E      DRDPVLL+SGIGGSI+++K KK    + RVWVRIFL++L F
Sbjct: 3  WIPCPCWGTNDDENAGEVADRDPVLLVSGIGGSILHSK-KKNSKSEIRVWVRIFLANLAF 62

Query: 65 REKLVSLYNPDT 74
          ++ L SLYNP T
Sbjct: 63 KQSLWSLYNPKT 73

[Close]

BLAST of Spg003255 vs. TAIR 10
Match: AT4G19860.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 58.2 bits (139), Expect = 4.7e-09
Identity = 29/62 (46.77%), Postives = 43/62 (69.35%), Query Frame = 0


Query: 12 RTRKPEPEPDRDPVLLLSGIGGSIINAKNKKLFGLQTRVWVRIFLSDLVFREKLVSLYNP 71
          R ++P  +P+ +PVLL+ GI GSI+NA + +  G + RVWVRIF +D  FR K+ S ++P
Sbjct: 21 RNQEPYVDPNLNPVLLVPGIAGSILNAVDHE-NGNEERVWVRIFGADHEFRTKMWSRFDP 80

Query: 72 DT 74
           T
Sbjct: 81 ST 81

The following BLAST results are available for this feature:

BLAST of Spg003255 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0051379.1	1.1e-25	80.28	phospholipase A(1) LCAT3 [Cucumis melo var. makuwa] >TYK23844.1 phospholipase A(...	[more]
XP_008441766.1	1.1e-25	80.28	PREDICTED: phospholipase A(1) LCAT3 [Cucumis melo]	[more]
XP_022144495.1	3.3e-25	76.06	phospholipase A(1) LCAT3 [Momordica charantia]	[more]
XP_022973913.1	2.2e-24	85.29	phospholipase A(1) LCAT3 [Cucurbita maxima]	[more]
XP_004138893.1	2.2e-24	76.06	phospholipase A(1) LCAT3 [Cucumis sativus]	[more]

BLAST of Spg003255 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93V61	5.2e-13	54.17	Phospholipase A(1) LCAT3 OS=Arabidopsis thaliana OX=3702 GN=LCAT3 PE=1 SV=1	[more]
Q71N54	6.6e-08	46.77	Lecithin-cholesterol acyltransferase-like 4 OS=Arabidopsis thaliana OX=3702 GN=L...	[more]

BLAST of Spg003255 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DJN1	5.5e-26	80.28	Phospholipase A(1) LCAT3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A1S3B3Q4	5.5e-26	80.28	phospholipase A(1) LCAT3 OS=Cucumis melo OX=3656 GN=LOC103485824 PE=4 SV=1	[more]
A0A6J1CRS5	1.6e-25	76.06	phospholipase A(1) LCAT3 OS=Momordica charantia OX=3673 GN=LOC111014171 PE=4 SV=...	[more]
A0A6J1IEK6	1.0e-24	85.29	phospholipase A(1) LCAT3 OS=Cucurbita maxima OX=3661 GN=LOC111472543 PE=4 SV=1	[more]
A0A0A0LHR5	1.0e-24	76.06	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G093800 PE=4 SV=1	[more]

BLAST of Spg003255 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G03310.1	3.7e-14	54.17	lecithin:cholesterol acyltransferase 3	[more]
AT4G19860.1	4.7e-09	46.77	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 84..103
None	No IPR available	PANTHER	PTHR11440:SF53	PHOSPHOLIPASE A(1) LCAT3	coord: 11..73
None	No IPR available	PANTHER	PTHR11440	LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATED	coord: 11..73

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg003255.1	Spg003255.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0008374	O-acyltransferase activity