Spg003076 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg003076 vs. NCBI nr
Match: XP_022995875.1 (ubiquitin carboxyl-terminal hydrolase MINDY-1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 940.6 bits (2430), Expect = 8.0e-270
Identity = 553/816 (67.77%), Postives = 582/816 (71.32%), Query Frame = 0

Query: 1   MASPSSSEEPQNPPQEQQQQPPKEKEKEKEKECLYKTKPIQFLGRSTPIVIVWPGGFCWI 60
           MASPSSSE+P NPP EQQQQ P    KEKE ECLYKTK IQFLGRSTPIV+    G C  
Sbjct: 1   MASPSSSEDPPNPPLEQQQQQP---PKEKENECLYKTKTIQFLGRSTPIVLQNDNGPC-- 60

Query: 61  GCIWNWRFGVGFIFFFCFTCAFLIAECLSAVISPSAPMLNRFVDYVFRNGDRTFSRNVLL 120
                                 L+A C                             NVLL
Sbjct: 61  ---------------------PLLAIC-----------------------------NVLL 120

Query: 121 LRNNLNLGPDATEVSQEKLLSLVAE----------------------------------- 180
           LRNNLNLGPDATEVSQEKLLSLVAE                                   
Sbjct: 121 LRNNLNLGPDATEVSQEKLLSLVAERLIDSNSNVDNKDAGFVENQQQNIADAIDLLPRLA 180

Query: 181 ----------RIDDFEFTPECAIFDLLDIPLYHGWIVDPQPWTDNEAVVVNLGLIFNMIC 240
                     RIDDFEFTPECAIFDLLDIPLYHGWIVDP                     
Sbjct: 181 TGIDVNIRFRRIDDFEFTPECAIFDLLDIPLYHGWIVDP--------------------- 240

Query: 241 NVAFRPLDHLDPQNLITKELCWRLQDHDTSSAIGSKSYNAIMEELVALETQQMEGQRNNN 300
                                   QDHDT+SAIGSKSYNAIMEELVALETQ ME Q +NN
Sbjct: 241 ------------------------QDHDTASAIGSKSYNAIMEELVALETQHMEDQCSNN 300

Query: 301 PEDDCIDFVAATTATLGVPSPSLSKALSFDESPRSVSDEQRIRKGDLEEEAELLRALKLS 360
           PEDDCIDFVAATTATLGVPSPSLSK  SFDESPRSVSDEQR+RKGDLEEEAELLRALKLS
Sbjct: 301 PEDDCIDFVAATTATLGVPSPSLSKNCSFDESPRSVSDEQRMRKGDLEEEAELLRALKLS 360

Query: 361 ESETPNSMENHGVANAEGDTNSIKLDEEPSPENVSVIDAVEKTIVKDICAAENNLNKQPD 420
           ES TP SM+NHGV ++ GDT + KLDEEPSPE VS+IDAVEK I+KDICAAENNLN+QP 
Sbjct: 361 ESVTPISMDNHGVVDSVGDTYATKLDEEPSPEKVSIIDAVEKAIIKDICAAENNLNQQPS 420

Query: 421 LSIEDSCKTSLTKHD-EDMSPFPDATEQVNSSLLKIDIGKHLDQTVESEGHKIGPDMLEK 480
           LSIEDSCKTSL+K D ED+S      EQV+SS LK D+GK L +TVESEGHKI PDML  
Sbjct: 421 LSIEDSCKTSLSKSDGEDVS-----QEQVSSSPLKADVGKPL-ETVESEGHKICPDML-- 480

Query: 481 DNHETLIETVPSSGRESPLVNENPEDISHKNDKSPSPAAFESVDHRPVDKEDPSELSGLS 540
           DNHETLIET P SGRES LVNEN +DISHKNDK+P PA FESVDH+PVDKED SELSGLS
Sbjct: 481 DNHETLIETDPISGRESSLVNENTDDISHKNDKTPLPAPFESVDHKPVDKEDSSELSGLS 540

Query: 541 SSVTCTDSAGGKTQCVEVDVPEGLTSNVGSEPLYEGEECILDPGSAVLEDREPVYEGEVV 600
           SSVTCTDSA GK QC  +DVP GLTS VGSEP+YEGEECILD  SAVL  +EPVYEGEVV
Sbjct: 541 SSVTCTDSADGKIQC--IDVPGGLTSTVGSEPMYEGEECILDARSAVLAAQEPVYEGEVV 600

Query: 601 LAEQGCGSSINFSNVQSKDEISPK------------------------QDGLKERELCVF 660
           LAEQGCGSSINF NV +K EISPK                        QDGLKERELCVF
Sbjct: 601 LAEQGCGSSINFPNVPAKYEISPKQGELIRNFLKNNASQLTFYGLFSLQDGLKERELCVF 660

Query: 661 FRNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNFKEFKVESHSN 720
           FRNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNFKEFKVESHSN
Sbjct: 661 FRNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNFKEFKVESHSN 706

Query: 721 ESWDEHNAMASTADYLASIDATKHAGLDLNSDLQLAIALQQQEFEQQPQRQNVQQSSSGG 747
           ESWDEHNAMASTADYLASIDATKHAGLDLNSDLQLAIALQQQEFEQQPQRQNVQQ SSGG
Sbjct: 721 ESWDEHNAMASTADYLASIDATKHAGLDLNSDLQLAIALQQQEFEQQPQRQNVQQPSSGG 706

BLAST of Spg003076 vs. NCBI nr
Match: XP_023532814.1 (ubiquitin carboxyl-terminal hydrolase MINDY-1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023532815.1 ubiquitin carboxyl-terminal hydrolase MINDY-1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 939.5 bits (2427), Expect = 1.8e-269
Identity = 550/815 (67.48%), Postives = 581/815 (71.29%), Query Frame = 0

Query: 1   MASPSSSEEPQNPPQEQQQQPPKEKEKEKEKECLYKTKPIQFLGRSTPIVIVWPGGFCWI 60
           MASPSSSE+P NPP EQQQQ P    KEKE ECLYKTK IQFLGRSTPIV+    G C  
Sbjct: 1   MASPSSSEDPPNPPLEQQQQQP---PKEKENECLYKTKTIQFLGRSTPIVLQNDNGPC-- 60

Query: 61  GCIWNWRFGVGFIFFFCFTCAFLIAECLSAVISPSAPMLNRFVDYVFRNGDRTFSRNVLL 120
                                 L+A C                             NVLL
Sbjct: 61  ---------------------PLLAIC-----------------------------NVLL 120

Query: 121 LRNNLNLGPDATEVSQEKLLSLVAE----------------------------------- 180
           LRNNLNLGPDATEVSQEKLLSLVAE                                   
Sbjct: 121 LRNNLNLGPDATEVSQEKLLSLVAERLIDSNSNVDNKDAGFVENQQQNIADAIDLLPRLA 180

Query: 181 ----------RIDDFEFTPECAIFDLLDIPLYHGWIVDPQPWTDNEAVVVNLGLIFNMIC 240
                     RIDDFEFTPECAIFDLLDIPLYHGWIVDP                     
Sbjct: 181 TGIDVNIRFRRIDDFEFTPECAIFDLLDIPLYHGWIVDP--------------------- 240

Query: 241 NVAFRPLDHLDPQNLITKELCWRLQDHDTSSAIGSKSYNAIMEELVALETQQMEGQRNNN 300
                                   QDHDT+SAIGSKSYNAIMEELVALETQ ME Q +NN
Sbjct: 241 ------------------------QDHDTASAIGSKSYNAIMEELVALETQHMEDQCSNN 300

Query: 301 PEDDCIDFVAATTATLGVPSPSLSKALSFDESPRSVSDEQRIRKGDLEEEAELLRALKLS 360
           PEDDCIDFVAATTATLGVPSPSLSK  SFDESPRSVSDEQR+RKGDLEEEAELLRALKLS
Sbjct: 301 PEDDCIDFVAATTATLGVPSPSLSKNCSFDESPRSVSDEQRMRKGDLEEEAELLRALKLS 360

Query: 361 ESETPNSMENHGVANAEGDTNSIKLDEEPSPENVSVIDAVEKTIVKDICAAENNLNKQPD 420
           ES TP SM+NHGV ++ GDT + KLDEEPSPE VS+IDAVEK I+K ICAAENNLN+QP 
Sbjct: 361 ESVTPISMDNHGVVDSVGDTYATKLDEEPSPEKVSIIDAVEKAIIKGICAAENNLNQQPS 420

Query: 421 LSIEDSCKTSLTKHDEDMSPFPDATEQVNSSLLKIDIGKHLDQTVESEGHKIGPDMLEKD 480
           LSIEDSC+TSL+K D++      + EQV+SS LK D+GK L +TVESEGHKI PDML  D
Sbjct: 421 LSIEDSCRTSLSKSDDE----GVSQEQVSSSPLKADVGKPL-ETVESEGHKICPDML--D 480

Query: 481 NHETLIETVPSSGRESPLVNENPEDISHKNDKSPSPAAFESVDHRPVDKEDPSELSGLSS 540
           NHETLIET P SGRES LVNEN +DISHKNDK+P PA FESVDH+PVDKED SELSGLSS
Sbjct: 481 NHETLIETDPISGRESSLVNENTDDISHKNDKTPLPAPFESVDHKPVDKEDSSELSGLSS 540

Query: 541 SVTCTDSAGGKTQCVEVDVPEGLTSNVGSEPLYEGEECILDPGSAVLEDREPVYEGEVVL 600
           SVTCTDSA GK QC  VDVP GLTS VGSEP+YEGEECILD  SAVL  +EPVYEGEVVL
Sbjct: 541 SVTCTDSADGKIQC--VDVPGGLTSTVGSEPMYEGEECILDARSAVLAAQEPVYEGEVVL 600

Query: 601 AEQGCGSSINFSNVQSKDEISPK------------------------QDGLKERELCVFF 660
           AEQGCGSSINF NV +KDEISPK                        QDGLKERELCVFF
Sbjct: 601 AEQGCGSSINFPNVPAKDEISPKQGELIRNFLKNNASQLTFYGLFSLQDGLKERELCVFF 660

Query: 661 RNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNFKEFKVESHSNE 720
           RNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNFKEFKVESHSNE
Sbjct: 661 RNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNFKEFKVESHSNE 706

Query: 721 SWDEHNAMASTADYLASIDATKHAGLDLNSDLQLAIALQQQEFEQQPQRQNVQQSSSGGS 747
           SWDEHNAMASTADYLASIDATKHAGLDLNSDLQLAIALQQQEFEQQPQRQNVQQ SSGGS
Sbjct: 721 SWDEHNAMASTADYLASIDATKHAGLDLNSDLQLAIALQQQEFEQQPQRQNVQQPSSGGS 706

BLAST of Spg003076 vs. NCBI nr
Match: KAG6606132.1 (Ubiquitin carboxyl-terminal hydrolase MINDY-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 934.1 bits (2413), Expect = 7.5e-268
Identity = 550/815 (67.48%), Postives = 579/815 (71.04%), Query Frame = 0

Query: 1   MASPSSSEEPQNPPQEQQQQPPKEKEKEKEKECLYKTKPIQFLGRSTPIVIVWPGGFCWI 60
           MASPSSSE+P NPP EQQQQ P    KEKE ECLYKTK IQFLGRSTPIV+    G C  
Sbjct: 1   MASPSSSEDPPNPPLEQQQQQP---PKEKENECLYKTKTIQFLGRSTPIVLQNDNGPC-- 60

Query: 61  GCIWNWRFGVGFIFFFCFTCAFLIAECLSAVISPSAPMLNRFVDYVFRNGDRTFSRNVLL 120
                                 L+A C                             NVLL
Sbjct: 61  ---------------------PLLAIC-----------------------------NVLL 120

Query: 121 LRNNLNLGPDATEVSQEKLLSLVAE----------------------------------- 180
           LRNNLNLGPDATEVSQEKLLSLVAE                                   
Sbjct: 121 LRNNLNLGPDATEVSQEKLLSLVAERLIDSNSNVDNKDAGFVENQQQNIADAIDLLPRLA 180

Query: 181 ----------RIDDFEFTPECAIFDLLDIPLYHGWIVDPQPWTDNEAVVVNLGLIFNMIC 240
                     RIDDFEFTPECAIFDLLDIPLYHGWIVDP                     
Sbjct: 181 TGIDVNIRFRRIDDFEFTPECAIFDLLDIPLYHGWIVDP--------------------- 240

Query: 241 NVAFRPLDHLDPQNLITKELCWRLQDHDTSSAIGSKSYNAIMEELVALETQQMEGQRNNN 300
                                   QDHDT+SAIGSKSYNAIMEELVALETQ ME Q +NN
Sbjct: 241 ------------------------QDHDTASAIGSKSYNAIMEELVALETQHMEDQCSNN 300

Query: 301 PEDDCIDFVAATTATLGVPSPSLSKALSFDESPRSVSDEQRIRKGDLEEEAELLRALKLS 360
           PEDDCIDFVAATTATLGVPSPSLSK  SFDESPRSVSDEQR RKGDLEEEAELLRALKLS
Sbjct: 301 PEDDCIDFVAATTATLGVPSPSLSKNCSFDESPRSVSDEQRKRKGDLEEEAELLRALKLS 360

Query: 361 ESETPNSMENHGVANAEGDTNSIKLDEEPSPENVSVIDAVEKTIVKDICAAENNLNKQPD 420
           ES TP SM+NHGV ++ GDTN+ KLDEEPSPE VS+IDAVEK I+KDICAAENNLN+QP 
Sbjct: 361 ESVTPISMDNHGVVDSVGDTNATKLDEEPSPEKVSIIDAVEKAIIKDICAAENNLNQQPS 420

Query: 421 LSIEDSCKTSLTKHDEDMSPFPDATEQVNSSLLKIDIGKHLDQTVESEGHKIGPDMLEKD 480
           LSIEDSC+TSL+K D++      + EQV+SS LK D+GK L +TVESEGHKI PDML  D
Sbjct: 421 LSIEDSCRTSLSKSDDE----GVSQEQVSSSPLKADVGKPL-ETVESEGHKICPDML--D 480

Query: 481 NHETLIETVPSSGRESPLVNENPEDISHKNDKSPSPAAFESVDHRPVDKEDPSELSGLSS 540
           NHETLIET P SGRES LVNEN +DISHKND    PA FESVDH+PVDKED SELSGLSS
Sbjct: 481 NHETLIETDPISGRESSLVNENTDDISHKND---MPAPFESVDHKPVDKEDSSELSGLSS 540

Query: 541 SVTCTDSAGGKTQCVEVDVPEGLTSNVGSEPLYEGEECILDPGSAVLEDREPVYEGEVVL 600
           SVTCTDSA GK QC  VDVP GLTS VGSEP+YEGEECILD  SAVL  +EPVYEGEVVL
Sbjct: 541 SVTCTDSADGKIQC--VDVPGGLTSTVGSEPMYEGEECILDARSAVLAAQEPVYEGEVVL 600

Query: 601 AEQGCGSSINFSNVQSKDEISPK------------------------QDGLKERELCVFF 660
           AEQGCGSSINF NV +KDEISPK                        QDGLKERELCVFF
Sbjct: 601 AEQGCGSSINFPNVPAKDEISPKQGELIRNFLKNNASQLTFYGLFSLQDGLKERELCVFF 660

Query: 661 RNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNFKEFKVESHSNE 720
           RNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNFKEFKVESHSNE
Sbjct: 661 RNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNFKEFKVESHSNE 703

Query: 721 SWDEHNAMASTADYLASIDATKHAGLDLNSDLQLAIALQQQEFEQQPQRQNVQQSSSGGS 747
           SWDEHNAMASTADYLASIDATKHAGLDLNSDLQLAIALQQQEFEQQPQRQNVQQ SSGGS
Sbjct: 721 SWDEHNAMASTADYLASIDATKHAGLDLNSDLQLAIALQQQEFEQQPQRQNVQQPSSGGS 703

BLAST of Spg003076 vs. NCBI nr
Match: KAG6579469.1 (Ubiquitin carboxyl-terminal hydrolase MINDY-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 933.3 bits (2411), Expect = 1.3e-267
Identity = 539/815 (66.13%), Postives = 572/815 (70.18%), Query Frame = 0

Query: 1   MASPSSSEEPQNPPQEQQQQPPKEKEKEKEKECLYKTKPIQFLGRSTPIVIVWPGGFCWI 60
           MASPSSS+EP N  QEQQQ P     KEKEKEC+YKTK IQFLGR TPIV+    G C  
Sbjct: 1   MASPSSSDEPPNQSQEQQQSP----VKEKEKECVYKTKAIQFLGRPTPIVLQNDNGPC-- 60

Query: 61  GCIWNWRFGVGFIFFFCFTCAFLIAECLSAVISPSAPMLNRFVDYVFRNGDRTFSRNVLL 120
                                 L+A C                             NVLL
Sbjct: 61  ---------------------PLLAIC-----------------------------NVLL 120

Query: 121 LRNNLNLGPDATEVSQEKLLSLVAE----------------------------------- 180
           LRNNLNLGPDATEVSQEKLLSLVAE                                   
Sbjct: 121 LRNNLNLGPDATEVSQEKLLSLVAERLIDSNSNVDNKDAGFVENQQQNIADAIDLLPRLA 180

Query: 181 ----------RIDDFEFTPECAIFDLLDIPLYHGWIVDPQPWTDNEAVVVNLGLIFNMIC 240
                     RIDDFEFTPECAIFDLLDIPLYHGWIVD                      
Sbjct: 181 TGIDVNIKFRRIDDFEFTPECAIFDLLDIPLYHGWIVDS--------------------- 240

Query: 241 NVAFRPLDHLDPQNLITKELCWRLQDHDTSSAIGSKSYNAIMEELVALETQQMEGQRNNN 300
                                   QDHDT++AIGSKSYNAIMEELVALETQ MEGQ  NN
Sbjct: 241 ------------------------QDHDTANAIGSKSYNAIMEELVALETQHMEGQCTNN 300

Query: 301 PEDDCIDFVAATTATLGVPSPSLSKALSFDESPRSVSDEQRIRKGDLEEEAELLRALKLS 360
           PEDDCIDFVAATTATLGVPSPSLSKA SFDESPRSV DEQ++RKGDLEEEAELLRALKLS
Sbjct: 301 PEDDCIDFVAATTATLGVPSPSLSKARSFDESPRSVFDEQQMRKGDLEEEAELLRALKLS 360

Query: 361 ESETPNSMENHGVANAEGDTNSIKLDEEPSPENVSVIDAVEKTIVKDICAAENNLNKQPD 420
           ESETP  M NHGVANA+GDTNS KLDEE SPE VSVID+V KT+ KD CAA+NNL++Q D
Sbjct: 361 ESETPILMSNHGVANADGDTNSTKLDEELSPEKVSVIDSVVKTVAKDTCAADNNLSQQSD 420

Query: 421 LSIEDSCKTSLTKHDEDMSPFPDATEQVNSSLLKIDIGKHLDQTVESEGHKIGPDMLEKD 480
            SI DSCK SL+K DED+SP  +ATE V+SS L  D GKHLD+T ESEGHKI PD+LEKD
Sbjct: 421 SSIADSCKISLSKTDEDVSPISNATEPVSSSPLTTDAGKHLDRTFESEGHKICPDLLEKD 480

Query: 481 NHETLIETVPSSGRESPLVNENPEDISHKNDKSPSPAAFESVDHRPVDKEDPSELSGLSS 540
           NHETL ETV SSGRE  LVN++PEDISH NDKS   AAFE+VDHRPVDKE P ELSGLS 
Sbjct: 481 NHETLTETVTSSGREGALVNDSPEDISHMNDKSAFSAAFETVDHRPVDKEGPIELSGLSP 540

Query: 541 SVTCTDSAGGKTQCVEVDVPEGLTSNVGSEPLYEGEECILDPGSAVLEDREPVYEGEVVL 600
           SVTCTDSAGGKTQC  +DVPEGL S VGSEP+YEGEECILDPGS+VLE REPVYEGEVV+
Sbjct: 541 SVTCTDSAGGKTQC--IDVPEGLNSIVGSEPIYEGEECILDPGSSVLEVREPVYEGEVVI 600

Query: 601 AEQGCGSSINFSNVQSKDEISPK------------------------QDGLKERELCVFF 660
           AEQGCGSS+NF +  SKDEISPK                        QDGLKERELCVFF
Sbjct: 601 AEQGCGSSVNFCSPPSKDEISPKQGELIRNFLKNNASQLTFYGLFCLQDGLKERELCVFF 660

Query: 661 RNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNFKEFKVESHSNE 720
           RNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMT NFKEFKVESHSNE
Sbjct: 661 RNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTSNFKEFKVESHSNE 712

Query: 721 SWDEHNAMASTADYLASIDATKHAGLDLNSDLQLAIALQQQEFEQQPQRQNVQQSSSGGS 747
           SWDE NA+ STADYLASIDA KHAGLD NSDLQLAIALQQQEFEQQPQRQNVQQ SSGGS
Sbjct: 721 SWDEQNALTSTADYLASIDAAKHAGLDFNSDLQLAIALQQQEFEQQPQRQNVQQPSSGGS 712

BLAST of Spg003076 vs. NCBI nr
Match: KAG7016938.1 (Ubiquitin carboxyl-terminal hydrolase MINDY-1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 932.2 bits (2408), Expect = 2.8e-267
Identity = 539/815 (66.13%), Postives = 572/815 (70.18%), Query Frame = 0

Query: 1   MASPSSSEEPQNPPQEQQQQPPKEKEKEKEKECLYKTKPIQFLGRSTPIVIVWPGGFCWI 60
           MASPSSS+EP N  QEQQQ P     KEKEKEC+YKTK IQFLGR TPIV+    G C  
Sbjct: 1   MASPSSSDEPPNQSQEQQQSP----VKEKEKECVYKTKAIQFLGRPTPIVLQNDNGPC-- 60

Query: 61  GCIWNWRFGVGFIFFFCFTCAFLIAECLSAVISPSAPMLNRFVDYVFRNGDRTFSRNVLL 120
                                 L+A C                             NVLL
Sbjct: 61  ---------------------PLLAIC-----------------------------NVLL 120

Query: 121 LRNNLNLGPDATEVSQEKLLSLVAE----------------------------------- 180
           LRNNLNLGPDATEVSQEKLLSLVAE                                   
Sbjct: 121 LRNNLNLGPDATEVSQEKLLSLVAERLIDSNSNVDNKDAGFVENQQQNIADAIDLLPRLA 180

Query: 181 ----------RIDDFEFTPECAIFDLLDIPLYHGWIVDPQPWTDNEAVVVNLGLIFNMIC 240
                     RIDDFEFTPECAIFDLLDIPLYHGWIVD                      
Sbjct: 181 TGIDVNIKFRRIDDFEFTPECAIFDLLDIPLYHGWIVDS--------------------- 240

Query: 241 NVAFRPLDHLDPQNLITKELCWRLQDHDTSSAIGSKSYNAIMEELVALETQQMEGQRNNN 300
                                   QDHDT++AIGSKSYNAIMEELVALETQ MEGQ  NN
Sbjct: 241 ------------------------QDHDTANAIGSKSYNAIMEELVALETQHMEGQCTNN 300

Query: 301 PEDDCIDFVAATTATLGVPSPSLSKALSFDESPRSVSDEQRIRKGDLEEEAELLRALKLS 360
           PEDDCIDFVAATTATLGVPSPSLSKA SFDESPRSV DEQ++RKGDLEEEAELLRALKLS
Sbjct: 301 PEDDCIDFVAATTATLGVPSPSLSKARSFDESPRSVFDEQQMRKGDLEEEAELLRALKLS 360

Query: 361 ESETPNSMENHGVANAEGDTNSIKLDEEPSPENVSVIDAVEKTIVKDICAAENNLNKQPD 420
           ESETP  M NHGVANA+GDTNS KLDEE SPE VSVID+V KT+ KD CAA+NNL++Q D
Sbjct: 361 ESETPILMINHGVANADGDTNSTKLDEELSPEKVSVIDSVVKTVAKDTCAADNNLSQQSD 420

Query: 421 LSIEDSCKTSLTKHDEDMSPFPDATEQVNSSLLKIDIGKHLDQTVESEGHKIGPDMLEKD 480
            SI DSCK SL+K DED+SP  +ATE V+SS L  D GKHLD+T ESEGHKI PD+LEKD
Sbjct: 421 SSIADSCKISLSKTDEDVSPISNATEPVSSSPLTTDAGKHLDRTFESEGHKICPDLLEKD 480

Query: 481 NHETLIETVPSSGRESPLVNENPEDISHKNDKSPSPAAFESVDHRPVDKEDPSELSGLSS 540
           NHETL ETV SSGRE  LVN++PEDISH NDKS   AAFE+VDHRPVDKE P ELSGLS 
Sbjct: 481 NHETLTETVTSSGREGALVNDSPEDISHMNDKSAFSAAFETVDHRPVDKEGPIELSGLSP 540

Query: 541 SVTCTDSAGGKTQCVEVDVPEGLTSNVGSEPLYEGEECILDPGSAVLEDREPVYEGEVVL 600
           SVTCTDSAGGKTQC  +DVPEGL S VGSEP+YEGEECILDPGS+VLE REPVYEGEVV+
Sbjct: 541 SVTCTDSAGGKTQC--IDVPEGLNSIVGSEPIYEGEECILDPGSSVLEVREPVYEGEVVI 600

Query: 601 AEQGCGSSINFSNVQSKDEISPK------------------------QDGLKERELCVFF 660
           AEQGCGSS+NF +  SKDEISPK                        QDGLKERELCVFF
Sbjct: 601 AEQGCGSSVNFCSPPSKDEISPKQGELIRNFLKNNASQLTFYGLFCLQDGLKERELCVFF 660

Query: 661 RNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNFKEFKVESHSNE 720
           RNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMT NFKEFKVESHSNE
Sbjct: 661 RNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTSNFKEFKVESHSNE 712

Query: 721 SWDEHNAMASTADYLASIDATKHAGLDLNSDLQLAIALQQQEFEQQPQRQNVQQSSSGGS 747
           SWDE NA+ STADYLASIDA KHAGLD NSDLQLAIALQQQEFEQQPQRQNVQQ SSGGS
Sbjct: 721 SWDEQNALTSTADYLASIDAAKHAGLDFNSDLQLAIALQQQEFEQQPQRQNVQQPSSGGS 712

BLAST of Spg003076 vs. ExPASy Swiss-Prot
Match: A3KQS4 (Ubiquitin carboxyl-terminal hydrolase MINDY-1 OS=Danio rerio OX=7955 GN=mindy1 PE=3 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 3.3e-16
Identity = 61/171 (35.67%), Postives = 85/171 (49.71%), Query Frame = 0

Query: 583 KERELCVFFRNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNFK- 642
           KE EL VFFRNNHFSTM K  G LYLL TDQG++ +  +VWE L+ V GD  F   +F+ 
Sbjct: 359 KEGELSVFFRNNHFSTMIKHKGHLYLLVTDQGFLQEESVVWESLHNVEGDGNFCDSDFRL 418

Query: 643 ----EFKVESHSNESWDEHNAMASTADYLASIDATKHAGLDLN--SDLQLAIALQQQEFE 702
               +    + +  S  +   M    DYL ++   +  G      SDL+LA  LQQ+E++
Sbjct: 419 CHPSQKPSAAAAQPSTQQQQQMQIDQDYLVAMSLQQEQGEAPGPLSDLELARQLQQEEYQ 478

Query: 703 QQPQRQNVQQSSSGGSSRLVVGPQVSRTSSKTPPSSSARTEAKSKEKCTVM 747
           Q   +Q  QQ  S G           R  + +P     R E K +  C ++
Sbjct: 479 QPQTQQQQQQQPSAGQ---------MRGQATSPQGGQRRREKKEETDCCIL 520

HSP 2 Score: 62.0 bits (149), Expect = 3.3e-08
Identity = 23/29 (79.31%), Postives = 27/29 (93.10%), Query Frame = 0

Query: 147 IDDFEFTPECAIFDLLDIPLYHGWIVDPQ 176
           + DFE+TPEC +FDLLDIPLYHGW+VDPQ
Sbjct: 269 VSDFEYTPECIVFDLLDIPLYHGWLVDPQ 297

BLAST of Spg003076 vs. ExPASy Swiss-Prot
Match: Q5BJQ2 (Ubiquitin carboxyl-terminal hydrolase MINDY-1 OS=Rattus norvegicus OX=10116 GN=Mindy1 PE=2 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 1.8e-14
Identity = 62/171 (36.26%), Postives = 89/171 (52.05%), Query Frame = 0

Query: 583 KERELCVFFRNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNF-- 642
           KE EL VFFRNNHFSTM K    LYLL TDQG++ +  +VWE L+ V+GD+ F   +F  
Sbjct: 321 KEDELSVFFRNNHFSTMTKHKSHLYLLVTDQGFLQEEQVVWESLHNVDGDSCFCDSDFHL 380

Query: 643 KEFKVESHSNE--SWDEHNAMASTADYLASIDATKH---AGLDLNSDLQLAIALQQQEFE 702
                +SH  E  S      +    DYL ++   +     G+   SDL+LA  LQQ+E++
Sbjct: 381 SHSLGKSHGAEGGSGSPEKQLQVDQDYLIALSLQQQQQPQGMLGLSDLELAQQLQQEEYQ 440

Query: 703 QQPQRQNVQQSSSGGSSRLVVGPQVSRTSSKTPPSSSARTEAKSKEKCTVM 747
           QQ   Q V+       +R    P    TS +  P+   R  +K++  C ++
Sbjct: 441 QQQAVQPVR-------TRAPSSPGRGATSGR--PAGERRQRSKTESDCVLL 482

HSP 2 Score: 63.2 bits (152), Expect = 1.5e-08
Identity = 31/69 (44.93%), Postives = 41/69 (59.42%), Query Frame = 0

Query: 147 IDDFEFTPECAIFDLLDIPLYHGWIVDPQPWTDNEAVVVNLGLIFNMICNVAFRPLDHLD 206
           + DFE+TPEC++FDLL +PLYHGW+VDPQ     EAV     L +N +         H  
Sbjct: 231 VSDFEYTPECSVFDLLGVPLYHGWLVDPQ---SPEAVSAVGKLSYNQLVEKII-ICKHSS 290

Query: 207 PQNLITKEL 216
             NL+T+ L
Sbjct: 291 DSNLVTEGL 295

BLAST of Spg003076 vs. ExPASy Swiss-Prot
Match: Q6PDI6 (Ubiquitin carboxyl-terminal hydrolase MINDY-2 OS=Mus musculus OX=10090 GN=Mindy2 PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 7.6e-13
Identity = 64/180 (35.56%), Postives = 95/180 (52.78%), Query Frame = 0

Query: 582 LKERELCVFFRNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNF- 641
           ++E ELCVFFRNNHFSTM K  GQLYLL TDQG++ +  +VWE L+ V+GD     GNF 
Sbjct: 413 VQEGELCVFFRNNHFSTMTKYKGQLYLLVTDQGFLTEEKIVWESLHNVDGD-----GNFC 472

Query: 642 -KEFKVESHSN-ESWDEHNAMASTADYLASID-ATKHAGLDLN--------SDLQLAIAL 701
             EF +   S+ E+  +        DYL ++    +    ++N        SDL+LA  L
Sbjct: 473 DSEFHLRPPSDPETVYKGQQDQIDQDYLMALSLQQEQQSQEINWEQIPEGISDLELAKKL 532

Query: 702 QQQE-------FEQQPQRQNVQQSSSGGSSRLVVGPQVSRTSSKTPPSSSARTEAKSKEK 743
           Q++E       +++Q Q Q V  +++  +      P  +  SS   P +S R   + +EK
Sbjct: 533 QEEEDRRASQYYQEQEQAQAVVTTTTPSTQAQQGQPAQASPSSIKQPGNSERKRKEPREK 587

HSP 2 Score: 47.4 bits (111), Expect = 8.4e-04
Identity = 48/169 (28.40%), Postives = 76/169 (44.97%), Query Frame = 0

Query: 15  QEQQQQPPKEKEKEKEK---ECLYKTKPIQFLGRSTPIVIVWPGGFCWIGCIWN-----W 74
           +E+++ P +     KE+   + +Y  K IQ+   +TPI+     G C +  I N     W
Sbjct: 198 KEEEEDPAQVLAASKERFPGQSVYHIKWIQWKEENTPIITQNENGPCPLLAILNVLLLAW 257

Query: 75  RFGVGFIFFFCFTCAFLIAECLSAVISPSAPMLNRFVDYVFRNGDRTFSRNVLLLRNNLN 134
           +  V          A  + E L   +  + P   + +  + R        + + + + L 
Sbjct: 258 K--VKLPPMMEIITAEQLMEYLGDYMLEAKP---KEISEIQRVNYEQNMSDAMAILHKLQ 317

Query: 135 LGPDATEVSQEKLLSLVAERIDDFEFTPECAIFDLLDIPLYHGWIVDPQ 176
            G D         +     R+  FE+TPEC +FDLLDIPLYHGW+VDPQ
Sbjct: 318 TGLDVN-------VRFTGVRV--FEYTPECIVFDLLDIPLYHGWLVDPQ 352

BLAST of Spg003076 vs. ExPASy Swiss-Prot
Match: Q8NBR6 (Ubiquitin carboxyl-terminal hydrolase MINDY-2 OS=Homo sapiens OX=9606 GN=MINDY2 PE=1 SV=2)

HSP 1 Score: 77.0 bits (188), Expect = 9.9e-13
Identity = 62/174 (35.63%), Postives = 92/174 (52.87%), Query Frame = 0

Query: 582 LKERELCVFFRNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNF- 641
           ++E ELCVFFRNNHFSTM K  GQLYLL TDQG++ +  +VWE L+ V+GD     GNF 
Sbjct: 435 VQEGELCVFFRNNHFSTMTKYKGQLYLLVTDQGFLTEEKVVWESLHNVDGD-----GNFC 494

Query: 642 -KEFKVESHSN-ESWDEHNAMASTADYLASID-ATKHAGLDLN--------SDLQLAIAL 701
             EF +   S+ E+  +        DYL ++    +    ++N        SDL+LA  L
Sbjct: 495 DSEFHLRPPSDPETVYKGQQDQIDQDYLMALSLQQEQQSQEINWEQIPEGISDLELAKKL 554

Query: 702 QQQEFEQQPQRQNVQQSSSGGSSRLVVGPQVSRTSSKTPPS--SSARTEAKSKE 742
           Q++E  +  Q    Q+ ++  ++      Q  + +  +P S   S  +E K KE
Sbjct: 555 QEEEDRRASQYYQEQEQAAAAAAAASTQAQQGQPAQASPSSGRQSGNSERKRKE 603

HSP 2 Score: 58.9 bits (141), Expect = 2.8e-07
Identity = 22/26 (84.62%), Postives = 25/26 (96.15%), Query Frame = 0

Query: 150 FEFTPECAIFDLLDIPLYHGWIVDPQ 176
           FE+TPEC +FDLLDIPLYHGW+VDPQ
Sbjct: 349 FEYTPECIVFDLLDIPLYHGWLVDPQ 374

BLAST of Spg003076 vs. ExPASy Swiss-Prot
Match: Q2KJ22 (Ubiquitin carboxyl-terminal hydrolase MINDY-1 OS=Bos taurus OX=9913 GN=MINDY1 PE=2 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 1.3e-12
Identity = 59/172 (34.30%), Postives = 84/172 (48.84%), Query Frame = 0

Query: 583 KERELCVFFRNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNF-- 642
           KE EL VFFRNNHFSTM K  G LYLL TDQG++ +  +VWE L+ V+GD+ F   +F  
Sbjct: 307 KEGELSVFFRNNHFSTMTKHKGHLYLLVTDQGFLQEEQVVWESLHNVDGDSCFCDSDFHL 366

Query: 643 --KEFKVESHSNESWDEHNAMASTADYLASIDATKH----AGLDLNSDLQLAIALQQQEF 702
                K       S           DYL ++   +      G    SDL+LA  LQQ+E+
Sbjct: 367 SHSPGKGPGTGGGSGSPEKQRQVDQDYLIALSLQQQQPPPQGTSGLSDLELAQQLQQEEY 426

Query: 703 EQQPQRQNVQQSSSGGSSRLVVGPQVSRTSSKTPPSSSARTEAKSKEKCTVM 747
           +Q        Q++    +R    PQ  R ++   P++  R   K +  C ++
Sbjct: 427 QQ-------HQAAQAAPAR-APSPQ-GRGAASGRPAAERRQRPKQESDCVLL 469

HSP 2 Score: 64.7 bits (156), Expect = 5.1e-09
Identity = 37/109 (33.94%), Postives = 55/109 (50.46%), Query Frame = 0

Query: 147 IDDFEFTPECAIFDLLDIPLYHGWIVDPQPWTDNEAVVVNLGLIFNMICNVAFRPLDHLD 206
           + DFE+TPEC++FDLL IPLYHGW+VDPQ     EAV     L +N +         H  
Sbjct: 217 VSDFEYTPECSVFDLLGIPLYHGWLVDPQ---SPEAVSAVGKLSYNQLVEKII-TCKHSS 276

Query: 207 PQNLITKELCWRLQDHDTSSAIGSKSYNAIMEELVALETQQMEGQRNNN 256
             NL+T+ L   + +    +     +Y+ + E   A +  ++     NN
Sbjct: 277 DTNLVTEGL---IAEQFLETTAAQLTYHGLCELTAAAKEGELSVFFRNN 318

BLAST of Spg003076 vs. ExPASy TrEMBL
Match: A0A6J1K549 (ubiquitin carboxyl-terminal hydrolase MINDY-1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491274 PE=4 SV=1)

HSP 1 Score: 940.6 bits (2430), Expect = 3.9e-270
Identity = 553/816 (67.77%), Postives = 582/816 (71.32%), Query Frame = 0

Query: 1   MASPSSSEEPQNPPQEQQQQPPKEKEKEKEKECLYKTKPIQFLGRSTPIVIVWPGGFCWI 60
           MASPSSSE+P NPP EQQQQ P    KEKE ECLYKTK IQFLGRSTPIV+    G C  
Sbjct: 1   MASPSSSEDPPNPPLEQQQQQP---PKEKENECLYKTKTIQFLGRSTPIVLQNDNGPC-- 60

Query: 61  GCIWNWRFGVGFIFFFCFTCAFLIAECLSAVISPSAPMLNRFVDYVFRNGDRTFSRNVLL 120
                                 L+A C                             NVLL
Sbjct: 61  ---------------------PLLAIC-----------------------------NVLL 120

Query: 121 LRNNLNLGPDATEVSQEKLLSLVAE----------------------------------- 180
           LRNNLNLGPDATEVSQEKLLSLVAE                                   
Sbjct: 121 LRNNLNLGPDATEVSQEKLLSLVAERLIDSNSNVDNKDAGFVENQQQNIADAIDLLPRLA 180

Query: 181 ----------RIDDFEFTPECAIFDLLDIPLYHGWIVDPQPWTDNEAVVVNLGLIFNMIC 240
                     RIDDFEFTPECAIFDLLDIPLYHGWIVDP                     
Sbjct: 181 TGIDVNIRFRRIDDFEFTPECAIFDLLDIPLYHGWIVDP--------------------- 240

Query: 241 NVAFRPLDHLDPQNLITKELCWRLQDHDTSSAIGSKSYNAIMEELVALETQQMEGQRNNN 300
                                   QDHDT+SAIGSKSYNAIMEELVALETQ ME Q +NN
Sbjct: 241 ------------------------QDHDTASAIGSKSYNAIMEELVALETQHMEDQCSNN 300

Query: 301 PEDDCIDFVAATTATLGVPSPSLSKALSFDESPRSVSDEQRIRKGDLEEEAELLRALKLS 360
           PEDDCIDFVAATTATLGVPSPSLSK  SFDESPRSVSDEQR+RKGDLEEEAELLRALKLS
Sbjct: 301 PEDDCIDFVAATTATLGVPSPSLSKNCSFDESPRSVSDEQRMRKGDLEEEAELLRALKLS 360

Query: 361 ESETPNSMENHGVANAEGDTNSIKLDEEPSPENVSVIDAVEKTIVKDICAAENNLNKQPD 420
           ES TP SM+NHGV ++ GDT + KLDEEPSPE VS+IDAVEK I+KDICAAENNLN+QP 
Sbjct: 361 ESVTPISMDNHGVVDSVGDTYATKLDEEPSPEKVSIIDAVEKAIIKDICAAENNLNQQPS 420

Query: 421 LSIEDSCKTSLTKHD-EDMSPFPDATEQVNSSLLKIDIGKHLDQTVESEGHKIGPDMLEK 480
           LSIEDSCKTSL+K D ED+S      EQV+SS LK D+GK L +TVESEGHKI PDML  
Sbjct: 421 LSIEDSCKTSLSKSDGEDVS-----QEQVSSSPLKADVGKPL-ETVESEGHKICPDML-- 480

Query: 481 DNHETLIETVPSSGRESPLVNENPEDISHKNDKSPSPAAFESVDHRPVDKEDPSELSGLS 540
           DNHETLIET P SGRES LVNEN +DISHKNDK+P PA FESVDH+PVDKED SELSGLS
Sbjct: 481 DNHETLIETDPISGRESSLVNENTDDISHKNDKTPLPAPFESVDHKPVDKEDSSELSGLS 540

Query: 541 SSVTCTDSAGGKTQCVEVDVPEGLTSNVGSEPLYEGEECILDPGSAVLEDREPVYEGEVV 600
           SSVTCTDSA GK QC  +DVP GLTS VGSEP+YEGEECILD  SAVL  +EPVYEGEVV
Sbjct: 541 SSVTCTDSADGKIQC--IDVPGGLTSTVGSEPMYEGEECILDARSAVLAAQEPVYEGEVV 600

Query: 601 LAEQGCGSSINFSNVQSKDEISPK------------------------QDGLKERELCVF 660
           LAEQGCGSSINF NV +K EISPK                        QDGLKERELCVF
Sbjct: 601 LAEQGCGSSINFPNVPAKYEISPKQGELIRNFLKNNASQLTFYGLFSLQDGLKERELCVF 660

Query: 661 FRNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNFKEFKVESHSN 720
           FRNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNFKEFKVESHSN
Sbjct: 661 FRNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNFKEFKVESHSN 706

Query: 721 ESWDEHNAMASTADYLASIDATKHAGLDLNSDLQLAIALQQQEFEQQPQRQNVQQSSSGG 747
           ESWDEHNAMASTADYLASIDATKHAGLDLNSDLQLAIALQQQEFEQQPQRQNVQQ SSGG
Sbjct: 721 ESWDEHNAMASTADYLASIDATKHAGLDLNSDLQLAIALQQQEFEQQPQRQNVQQPSSGG 706

BLAST of Spg003076 vs. ExPASy TrEMBL
Match: A0A6J1I1B4 (ubiquitin carboxyl-terminal hydrolase MINDY-1-like OS=Cucurbita maxima OX=3661 GN=LOC111468978 PE=4 SV=1)

HSP 1 Score: 929.9 bits (2402), Expect = 6.8e-267
Identity = 538/815 (66.01%), Postives = 571/815 (70.06%), Query Frame = 0

Query: 1   MASPSSSEEPQNPPQEQQQQPPKEKEKEKEKECLYKTKPIQFLGRSTPIVIVWPGGFCWI 60
           MASPSSS+EP N  QEQQQ P     KEKEKEC+YKTK +QFLGR TPIV+    G C  
Sbjct: 1   MASPSSSDEPPNQSQEQQQPP----VKEKEKECVYKTKAVQFLGRPTPIVLQNDNGPC-- 60

Query: 61  GCIWNWRFGVGFIFFFCFTCAFLIAECLSAVISPSAPMLNRFVDYVFRNGDRTFSRNVLL 120
                                 L+A C                             NVLL
Sbjct: 61  ---------------------PLLAIC-----------------------------NVLL 120

Query: 121 LRNNLNLGPDATEVSQEKLLSLVAE----------------------------------- 180
           LRNNLNLGPDATEVSQEKLLSLVAE                                   
Sbjct: 121 LRNNLNLGPDATEVSQEKLLSLVAERLIDSNSNVDNKDAGFVENQQQNIADAIDLLPRLA 180

Query: 181 ----------RIDDFEFTPECAIFDLLDIPLYHGWIVDPQPWTDNEAVVVNLGLIFNMIC 240
                     RIDDFEFTPECAIFDLLDIPLYHGWIVD                      
Sbjct: 181 TGIDVNIKFRRIDDFEFTPECAIFDLLDIPLYHGWIVDS--------------------- 240

Query: 241 NVAFRPLDHLDPQNLITKELCWRLQDHDTSSAIGSKSYNAIMEELVALETQQMEGQRNNN 300
                                   QDHDT++AIGSKSYNAIMEELVALETQ MEGQ  NN
Sbjct: 241 ------------------------QDHDTANAIGSKSYNAIMEELVALETQHMEGQCTNN 300

Query: 301 PEDDCIDFVAATTATLGVPSPSLSKALSFDESPRSVSDEQRIRKGDLEEEAELLRALKLS 360
           PEDDCIDFVAATTATLGVPSPSLSKA SFDESPRSV DEQ++RKGDLEEEAELLRALKLS
Sbjct: 301 PEDDCIDFVAATTATLGVPSPSLSKARSFDESPRSVFDEQQMRKGDLEEEAELLRALKLS 360

Query: 361 ESETPNSMENHGVANAEGDTNSIKLDEEPSPENVSVIDAVEKTIVKDICAAENNLNKQPD 420
           ESETP  M NHGVANAEGDT S KLDEE SPE VSVID+V KT+ KD CAA+NNL++Q D
Sbjct: 361 ESETPILMSNHGVANAEGDTTSTKLDEELSPEKVSVIDSVVKTVAKDTCAADNNLSQQSD 420

Query: 421 LSIEDSCKTSLTKHDEDMSPFPDATEQVNSSLLKIDIGKHLDQTVESEGHKIGPDMLEKD 480
            SI DSCK SL+K DED+ P  +ATE V+SS L  D GKHLDQTVESEGHKI PD+LEKD
Sbjct: 421 SSIADSCKISLSKTDEDVFPISNATELVSSSPLTTDAGKHLDQTVESEGHKICPDLLEKD 480

Query: 481 NHETLIETVPSSGRESPLVNENPEDISHKNDKSPSPAAFESVDHRPVDKEDPSELSGLSS 540
           NHETL E+V SSGRE  LVN++PEDISH NDKS   AAFE+VDHRPVDKE P ELSGLS 
Sbjct: 481 NHETLTESVTSSGREGALVNDSPEDISHMNDKSAFSAAFETVDHRPVDKEGPIELSGLSP 540

Query: 541 SVTCTDSAGGKTQCVEVDVPEGLTSNVGSEPLYEGEECILDPGSAVLEDREPVYEGEVVL 600
           SVTCTDSAGGK QC  +DVPEGLTS VGSEP+YEGEECILDPGS+VLE REPVYEGEVV+
Sbjct: 541 SVTCTDSAGGKIQC--IDVPEGLTSIVGSEPIYEGEECILDPGSSVLEVREPVYEGEVVI 600

Query: 601 AEQGCGSSINFSNVQSKDEISPK------------------------QDGLKERELCVFF 660
           AEQGCGSS+NF +  SKDEISPK                        QDGLKERELCVFF
Sbjct: 601 AEQGCGSSVNFCSPPSKDEISPKQGELIRNFLKNNASQLTFYGLFCLQDGLKERELCVFF 660

Query: 661 RNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNFKEFKVESHSNE 720
           RNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMT NFKEFKVESHSNE
Sbjct: 661 RNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTSNFKEFKVESHSNE 712

Query: 721 SWDEHNAMASTADYLASIDATKHAGLDLNSDLQLAIALQQQEFEQQPQRQNVQQSSSGGS 747
           SWDE NA+ STADYLASIDA KHAGLD NSDLQLAIALQQQEFEQQPQRQNVQQ SSGGS
Sbjct: 721 SWDEQNALTSTADYLASIDAAKHAGLDFNSDLQLAIALQQQEFEQQPQRQNVQQPSSGGS 712

BLAST of Spg003076 vs. ExPASy TrEMBL
Match: A0A6J1K344 (ubiquitin carboxyl-terminal hydrolase MINDY-1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491274 PE=4 SV=1)

HSP 1 Score: 928.7 bits (2399), Expect = 1.5e-266
Identity = 549/816 (67.28%), Postives = 578/816 (70.83%), Query Frame = 0

Query: 1   MASPSSSEEPQNPPQEQQQQPPKEKEKEKEKECLYKTKPIQFLGRSTPIVIVWPGGFCWI 60
           MASPSSSE+P NPP EQQQQ P    KEKE ECLYKTK IQFLGRSTPIV+    G C  
Sbjct: 1   MASPSSSEDPPNPPLEQQQQQP---PKEKENECLYKTKTIQFLGRSTPIVLQNDNGPC-- 60

Query: 61  GCIWNWRFGVGFIFFFCFTCAFLIAECLSAVISPSAPMLNRFVDYVFRNGDRTFSRNVLL 120
                                 L+A C                             NVLL
Sbjct: 61  ---------------------PLLAIC-----------------------------NVLL 120

Query: 121 LRNNLNLGPDATEVSQEKLLSLVAE----------------------------------- 180
           LRNNLNLGPDATEVSQEKLLSLVAE                                   
Sbjct: 121 LRNNLNLGPDATEVSQEKLLSLVAERLIDSNSNVDNKDAGFVENQQQNIADAIDLLPRLA 180

Query: 181 ----------RIDDFEFTPECAIFDLLDIPLYHGWIVDPQPWTDNEAVVVNLGLIFNMIC 240
                     RIDDFEFTPECAIFDLLDIPLYHGWIVDP                     
Sbjct: 181 TGIDVNIRFRRIDDFEFTPECAIFDLLDIPLYHGWIVDP--------------------- 240

Query: 241 NVAFRPLDHLDPQNLITKELCWRLQDHDTSSAIGSKSYNAIMEELVALETQQMEGQRNNN 300
                                   QDHDT+SAIGSKSYNAIMEELVALETQ ME Q +NN
Sbjct: 241 ------------------------QDHDTASAIGSKSYNAIMEELVALETQHMEDQCSNN 300

Query: 301 PEDDCIDFVAATTATLGVPSPSLSKALSFDESPRSVSDEQRIRKGDLEEEAELLRALKLS 360
           PEDDCIDFVAATTATLGVPSPSLSK  SFDESPRSVSDEQR+RKGDLEEEAELLRALKLS
Sbjct: 301 PEDDCIDFVAATTATLGVPSPSLSKNCSFDESPRSVSDEQRMRKGDLEEEAELLRALKLS 360

Query: 361 ESETPNSMENHGVANAEGDTNSIKLDEEPSPENVSVIDAVEKTIVKDICAAENNLNKQPD 420
           ES TP SM+NHGV ++ GDT + KLDEEPSPE VS+IDAVEK I+KDICAAENNLN+QP 
Sbjct: 361 ESVTPISMDNHGVVDSVGDTYATKLDEEPSPEKVSIIDAVEKAIIKDICAAENNLNQQPS 420

Query: 421 LSIEDSCKTSLTKHD-EDMSPFPDATEQVNSSLLKIDIGKHLDQTVESEGHKIGPDMLEK 480
           LSIEDSCKTSL+K D ED+S      EQV+SS LK D+GK L +TVESEGHKI PDML  
Sbjct: 421 LSIEDSCKTSLSKSDGEDVS-----QEQVSSSPLKADVGKPL-ETVESEGHKICPDML-- 480

Query: 481 DNHETLIETVPSSGRESPLVNENPEDISHKNDKSPSPAAFESVDHRPVDKEDPSELSGLS 540
           DNHETLIET P SGRES LVNEN +DISHKNDK+P PA FESVDH+PVDKED SELSGLS
Sbjct: 481 DNHETLIETDPISGRESSLVNENTDDISHKNDKTPLPAPFESVDHKPVDKEDSSELSGLS 540

Query: 541 SSVTCTDSAGGKTQCVEVDVPEGLTSNVGSEPLYEGEECILDPGSAVLEDREPVYEGEVV 600
           SSVTCTDSA GK QC  +DVP GLTS VGSEP+YEGEECILD  SAVL  +EPVYEGEVV
Sbjct: 541 SSVTCTDSADGKIQC--IDVPGGLTSTVGSEPMYEGEECILDARSAVLAAQEPVYEGEVV 600

Query: 601 LAEQGCGSSINFSNVQSKDEISPK------------------------QDGLKERELCVF 660
           LAEQGCGSSINF NV +K EISPK                        QDGLKERELCVF
Sbjct: 601 LAEQGCGSSINFPNVPAKYEISPKQGELIRNFLKNNASQLTFYGLFSLQDGLKERELCVF 660

Query: 661 FRNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNFKEFKVESHSN 720
           FRNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNFKEFKVESHSN
Sbjct: 661 FRNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNFKEFKVESHSN 702

Query: 721 ESWDEHNAMASTADYLASIDATKHAGLDLNSDLQLAIALQQQEFEQQPQRQNVQQSSSGG 747
           ESWDEHNAM    DYLASIDATKHAGLDLNSDLQLAIALQQQEFEQQPQRQNVQQ SSGG
Sbjct: 721 ESWDEHNAM----DYLASIDATKHAGLDLNSDLQLAIALQQQEFEQQPQRQNVQQPSSGG 702

BLAST of Spg003076 vs. ExPASy TrEMBL
Match: A0A6J1H4V0 (ubiquitin carboxyl-terminal hydrolase MINDY-1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459599 PE=4 SV=1)

HSP 1 Score: 927.9 bits (2397), Expect = 2.6e-266
Identity = 546/815 (66.99%), Postives = 577/815 (70.80%), Query Frame = 0

Query: 1   MASPSSSEEPQNPPQEQQQQPPKEKEKEKEKECLYKTKPIQFLGRSTPIVIVWPGGFCWI 60
           MASPSSSE+P NPP EQQQQ P    KEKE ECLYKTK IQFLGRSTPIV+    G C  
Sbjct: 1   MASPSSSEDPPNPPLEQQQQQP---PKEKENECLYKTKTIQFLGRSTPIVLQNDNGPC-- 60

Query: 61  GCIWNWRFGVGFIFFFCFTCAFLIAECLSAVISPSAPMLNRFVDYVFRNGDRTFSRNVLL 120
                                 L+A C                             NVLL
Sbjct: 61  ---------------------PLLAIC-----------------------------NVLL 120

Query: 121 LRNNLNLGPDATEVSQEKLLSLVAE----------------------------------- 180
           LRNNLNLGPDATEVSQEKLLSLVAE                                   
Sbjct: 121 LRNNLNLGPDATEVSQEKLLSLVAERLIDSNSNVDNKDAGFVENQQQNIADAIDLLPRLA 180

Query: 181 ----------RIDDFEFTPECAIFDLLDIPLYHGWIVDPQPWTDNEAVVVNLGLIFNMIC 240
                     RIDDFEFTPECAIFDLLDIPLYHGWIVDP                     
Sbjct: 181 TGIDVNIRFRRIDDFEFTPECAIFDLLDIPLYHGWIVDP--------------------- 240

Query: 241 NVAFRPLDHLDPQNLITKELCWRLQDHDTSSAIGSKSYNAIMEELVALETQQMEGQRNNN 300
                                   QDHDT+SAIGSKSYNAIMEELVALETQ ME Q +NN
Sbjct: 241 ------------------------QDHDTASAIGSKSYNAIMEELVALETQHMEDQCSNN 300

Query: 301 PEDDCIDFVAATTATLGVPSPSLSKALSFDESPRSVSDEQRIRKGDLEEEAELLRALKLS 360
           PEDDCIDFVAATTATLGVPSPSLSK  SFDESPRSVSDEQR+RKGDLEEEAELLRALKLS
Sbjct: 301 PEDDCIDFVAATTATLGVPSPSLSKNCSFDESPRSVSDEQRMRKGDLEEEAELLRALKLS 360

Query: 361 ESETPNSMENHGVANAEGDTNSIKLDEEPSPENVSVIDAVEKTIVKDICAAENNLNKQPD 420
           ES TP SM+NHGV ++ GDTN+ KLDEEPSPE VS+IDAVEK I+KDICAA NNLN+QP 
Sbjct: 361 ESVTPISMDNHGVVDSVGDTNATKLDEEPSPEKVSIIDAVEKAIIKDICAAGNNLNQQPS 420

Query: 421 LSIEDSCKTSLTKHDEDMSPFPDATEQVNSSLLKIDIGKHLDQTVESEGHKIGPDMLEKD 480
           LSIEDSC+TSL+K D++      + EQV+SS LK D+GK L +TVESEGHKI PDML  D
Sbjct: 421 LSIEDSCRTSLSKSDDE----GVSQEQVSSSPLKADVGKPL-ETVESEGHKICPDML--D 480

Query: 481 NHETLIETVPSSGRESPLVNENPEDISHKNDKSPSPAAFESVDHRPVDKEDPSELSGLSS 540
           NHETLIET P SGRES LVNEN +DISHKND    PA FESVDH+PVDKED SEL GLSS
Sbjct: 481 NHETLIETDPISGRESSLVNENTDDISHKND---MPAPFESVDHKPVDKEDSSELLGLSS 540

Query: 541 SVTCTDSAGGKTQCVEVDVPEGLTSNVGSEPLYEGEECILDPGSAVLEDREPVYEGEVVL 600
           SVTCTDSA GK QC  VDVP GLTS VGSEP+YEGEECILD  S VL  +EPVYEGEVVL
Sbjct: 541 SVTCTDSADGKIQC--VDVPGGLTSTVGSEPMYEGEECILDARSVVLAAQEPVYEGEVVL 600

Query: 601 AEQGCGSSINFSNVQSKDEISPK------------------------QDGLKERELCVFF 660
           AEQGCGSSINF NV +KDEISPK                        QDGLKERELCVFF
Sbjct: 601 AEQGCGSSINFPNVPAKDEISPKQGELIRNFLKNNASQLTFYGLFSLQDGLKERELCVFF 660

Query: 661 RNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNFKEFKVESHSNE 720
           RNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNFKEFKVESHSNE
Sbjct: 661 RNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNFKEFKVESHSNE 703

Query: 721 SWDEHNAMASTADYLASIDATKHAGLDLNSDLQLAIALQQQEFEQQPQRQNVQQSSSGGS 747
           SWDEHNAMASTADYLASIDATKHAGLDLNSDLQLAIALQQQEFEQQPQRQNVQQ S+GGS
Sbjct: 721 SWDEHNAMASTADYLASIDATKHAGLDLNSDLQLAIALQQQEFEQQPQRQNVQQPSTGGS 703

BLAST of Spg003076 vs. ExPASy TrEMBL
Match: A0A6J1E882 (ubiquitin carboxyl-terminal hydrolase MINDY-1-like OS=Cucurbita moschata OX=3662 GN=LOC111430296 PE=4 SV=1)

HSP 1 Score: 927.2 bits (2395), Expect = 4.4e-266
Identity = 538/815 (66.01%), Postives = 570/815 (69.94%), Query Frame = 0

Query: 1   MASPSSSEEPQNPPQEQQQQPPKEKEKEKEKECLYKTKPIQFLGRSTPIVIVWPGGFCWI 60
           MASPSSS+EP N  QEQQQ P     KEKEKEC+YKTK IQFLGR TPIV+    G C  
Sbjct: 1   MASPSSSDEPPNQSQEQQQSP----VKEKEKECVYKTKAIQFLGRPTPIVLQNDNGPC-- 60

Query: 61  GCIWNWRFGVGFIFFFCFTCAFLIAECLSAVISPSAPMLNRFVDYVFRNGDRTFSRNVLL 120
                                 L+A C                             NVLL
Sbjct: 61  ---------------------PLLAIC-----------------------------NVLL 120

Query: 121 LRNNLNLGPDATEVSQEKLLSLVAE----------------------------------- 180
           LRNNLNLGPDATEVSQEKLLSLVAE                                   
Sbjct: 121 LRNNLNLGPDATEVSQEKLLSLVAERLIDSNSNVDNKDAGFVENQQQNIADAIDLLPRLA 180

Query: 181 ----------RIDDFEFTPECAIFDLLDIPLYHGWIVDPQPWTDNEAVVVNLGLIFNMIC 240
                     RIDDFEFTPECAIFDLLDIPLYHGWIVD                      
Sbjct: 181 TGIDVNIKFRRIDDFEFTPECAIFDLLDIPLYHGWIVDS--------------------- 240

Query: 241 NVAFRPLDHLDPQNLITKELCWRLQDHDTSSAIGSKSYNAIMEELVALETQQMEGQRNNN 300
                                   QDHDT++AIGSKSYNAIMEELVALETQ MEGQ  NN
Sbjct: 241 ------------------------QDHDTANAIGSKSYNAIMEELVALETQHMEGQCTNN 300

Query: 301 PEDDCIDFVAATTATLGVPSPSLSKALSFDESPRSVSDEQRIRKGDLEEEAELLRALKLS 360
           PEDDCIDFVAATTATLGVPSPSLSKA SFDESPRSV DEQ++RKGDLEEEAELLRALKLS
Sbjct: 301 PEDDCIDFVAATTATLGVPSPSLSKARSFDESPRSVFDEQQMRKGDLEEEAELLRALKLS 360

Query: 361 ESETPNSMENHGVANAEGDTNSIKLDEEPSPENVSVIDAVEKTIVKDICAAENNLNKQPD 420
           ESETP  M NHGVANA+GDTNS KLDEE SPE VSVID+V KT+ KD CAA+NNL++Q D
Sbjct: 361 ESETPILMSNHGVANADGDTNSTKLDEELSPEKVSVIDSVVKTVAKDTCAADNNLSQQSD 420

Query: 421 LSIEDSCKTSLTKHDEDMSPFPDATEQVNSSLLKIDIGKHLDQTVESEGHKIGPDMLEKD 480
            SI DSCK  L+K DED+SP  +ATE V+SS L  D GKHLD TVESEGHKI PD+LEKD
Sbjct: 421 SSIADSCKIPLSKTDEDVSPISNATEPVSSSPLTTDAGKHLDWTVESEGHKICPDLLEKD 480

Query: 481 NHETLIETVPSSGRESPLVNENPEDISHKNDKSPSPAAFESVDHRPVDKEDPSELSGLSS 540
           NHETL ETV SSGRE  LVN++PEDISH NDKS   AAFE+VDHRPVDKE P ELSGLS 
Sbjct: 481 NHETLTETVTSSGREGALVNDSPEDISHMNDKSAFSAAFETVDHRPVDKEGPIELSGLSP 540

Query: 541 SVTCTDSAGGKTQCVEVDVPEGLTSNVGSEPLYEGEECILDPGSAVLEDREPVYEGEVVL 600
           SVT TDSAGGKTQC  +DVPEGL S VGSEP+YEGEECILDPGS+VLE REPVYEGEVV+
Sbjct: 541 SVTRTDSAGGKTQC--IDVPEGLNSIVGSEPIYEGEECILDPGSSVLEVREPVYEGEVVI 600

Query: 601 AEQGCGSSINFSNVQSKDEISPK------------------------QDGLKERELCVFF 660
           AEQGCGSS+NF +  SKDEISPK                        QDGLKERELCVFF
Sbjct: 601 AEQGCGSSVNFCSPPSKDEISPKQGELIRNFLKNNASQLTFYGLFCLQDGLKERELCVFF 660

Query: 661 RNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNFKEFKVESHSNE 720
           RNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMT NFKEFKVESHSNE
Sbjct: 661 RNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTSNFKEFKVESHSNE 712

Query: 721 SWDEHNAMASTADYLASIDATKHAGLDLNSDLQLAIALQQQEFEQQPQRQNVQQSSSGGS 747
           SWDE NA+ STADYLASIDA KHAGLD NSDLQLAIALQQQEFEQQPQRQNVQQ SSGGS
Sbjct: 721 SWDEQNALTSTADYLASIDAAKHAGLDFNSDLQLAIALQQQEFEQQPQRQNVQQPSSGGS 712

BLAST of Spg003076 vs. TAIR 10
Match: AT4G11860.1 (Protein of unknown function (DUF544) )

HSP 1 Score: 393.7 bits (1010), Expect = 3.4e-109
Identity = 312/828 (37.68%), Postives = 403/828 (48.67%), Query Frame = 0

Query: 1   MASPSSSEEPQNPP---QEQQQQPPKEKEKEKEKECLYKTKPIQFLGRSTPIVIVWPGGF 60
           MA+ SSSE P       QE  QQ    KE   E+E LYKTK IQFLGR+TPI++    G 
Sbjct: 1   MATASSSESPSEETQTNQEAMQQQDLPKETTTEEEILYKTKSIQFLGRTTPIILQNENGP 60

Query: 61  CWIGCIWNWRFGVGFIFFFCFTCAFLIAECLSAVISPSAPMLNRFVDYVFRNGDRTFSRN 120
           C                        L+A C                             N
Sbjct: 61  C-----------------------PLLAIC-----------------------------N 120

Query: 121 VLLLRNNLNLGPDATEVSQEKLLSLVAE-------------------------------- 180
           VLLLRNNLNL PD  EVSQE+L+SLV +                                
Sbjct: 121 VLLLRNNLNLNPDCYEVSQERLMSLVVDRLIDSNSKVNNKDEGYIENQQQNIADAIDLLP 180

Query: 181 -------------RIDDFEFTPECAIFDLLDIPLYHGWIVDPQPWTDNEAVVVNLGLIFN 240
                        RIDDFEFTPECAIFDLLDIPLYHGWIVDP                  
Sbjct: 181 RLTTGIDVNIKFRRIDDFEFTPECAIFDLLDIPLYHGWIVDP------------------ 240

Query: 241 MICNVAFRPLDHLDPQNLITKELCWRLQDHDTSSAIGSKSYNAIMEELVALETQQMEGQR 300
                                      QD + ++AIGSKSYNA+M ELVALETQ +E Q 
Sbjct: 241 ---------------------------QDVEAANAIGSKSYNALMGELVALETQNVEAQG 300

Query: 301 NNNPEDDCIDFVAATTATLGVPSPSLSKALSFDESPRSVSDEQRIRKGDLEEEAELLRAL 360
           + NP +D +DF AATTA LGVPSP LSK  SFD+SP + ++ +R+RKGDLEEE ELL+AL
Sbjct: 301 DQNPGEDSVDFAAATTAVLGVPSPCLSKTRSFDDSPPAAAELRRMRKGDLEEETELLQAL 360

Query: 361 KLSESETPNSMENHGVANAEGDTNSIKLDEEPSPENVSVIDAVEKTIVKDICAAENNLNK 420
           +LS+ +  +   N    +   D+     D  P+  +++ I  +++    D  A+EN+ N 
Sbjct: 361 QLSQGQGNDPTPNTNGDSTNQDSAFTFSDASPTSTHITNISQLDQFKTDDDKASENDGN- 420

Query: 421 QPDLSIEDSCKTSLTKHDEDMSPFPDATEQVNSSLLKIDIGKHLDQTVESEGHKIGPDML 480
              +       T++T   ED++                    H   + +  G +   D  
Sbjct: 421 ---MIKVGKFPTTITTKSEDLN--------------------HDQLSSKQSGGETACD-- 480

Query: 481 EKDNHETLIETVPSSGRESPLVNENPEDISHKNDKSPSPAAFESVDHRPVDKEDPSELSG 540
                   +E V SS        E   D++     S      ES       K + S  S 
Sbjct: 481 --------VENVSSS-------KEAIVDVTSSEALSVDKGNLESA------KSESSSESV 540

Query: 541 LSSSVTCTDSAGGKTQCVEVDVPEGLTSNVGS-EPLYEGEECILDPGSAVLEDREPVYEG 600
           L S     D      +    D P      V + EP+YEGEEC+ +    V  D+EPVYEG
Sbjct: 541 LKSDAASID-PDLSCRSQHDDAPNAFIPPVSTDEPMYEGEECV-NTVPPVCADKEPVYEG 600

Query: 601 EVVLAEQ------GCGSSINFSNVQSKDE-------------------ISPKQDGLKERE 660
           E +L ++       C S    ++  + +E                   +   Q+GLKERE
Sbjct: 601 ESLLGKRVEKDVGDCSSEGRATDGLTAEEGELIRNFMKNSASQLTFCGLFRLQEGLKERE 660

Query: 661 LCVFFRNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEKLNEVNGDTLFMTGNFKEFKVE 720
           LCVFFRNNHF TMFK +G+LYLLATDQGY++QPDLVWEKLNEVNGDT FMT  FK+F ++
Sbjct: 661 LCVFFRNNHFCTMFKYEGELYLLATDQGYLNQPDLVWEKLNEVNGDTAFMTATFKDFTID 682

Query: 721 SH---SNESWDEHNAMASTADYLASIDATKHAGLDLNSDLQLAIALQQQEFEQQPQRQN- 747
           S    ++ +WDE NA+ +TADYLASI+    AG+D+NSDLQLAIALQQQEFE Q  R N 
Sbjct: 721 SSTGGASGTWDERNAVTNTADYLASINNVADAGIDVNSDLQLAIALQQQEFEDQSPRSNP 682

BLAST of Spg003076 vs. TAIR 10
Match: AT4G22960.1 (Protein of unknown function (DUF544) )

HSP 1 Score: 117.9 bits (294), Expect = 3.6e-26
Identity = 160/610 (26.23%), Postives = 236/610 (38.69%), Query Frame = 0

Query: 117 NVLLLRNNLNLGPDATEVSQEKLLSLVA----------------------------ERID 176
           NVL+L++ +NL    ++VS+E L++LV                             ERI 
Sbjct: 45  NVLILKHGINLDTYNSQVSEEDLVNLVIDVLDDTDEYIDIGLIRKLACGINVDLKFERIT 104

Query: 177 DFEFTPECAIFDLLDIPLYHGWIVDPQPWTDNEAVVVNLGLIFNMICNVAFRPLDHLDPQ 236
           DFE TPE  IF  L IPLYHGW+VDP   TD E V                         
Sbjct: 105 DFELTPELDIFRSLKIPLYHGWLVDP---TDVEIV------------------------- 164

Query: 237 NLITKELCWRLQDHDTSSAIGSKSYNAIMEELVALETQQMEGQRNNNPEDDCIDFVAATT 296
                            +AIG +S + +   L  L+TQ +            +DF AA+ 
Sbjct: 165 -----------------AAIGGRSRDELEIALTTLDTQTVSS----------VDF-AASA 224

Query: 297 ATLGVPSPSLSKALSFDESPRSVSDEQRIRKGDLEEEAELLRALKLSESET--PNSMENH 356
             LG                        + KGD+EE+  + +AL LSE E     S +  
Sbjct: 225 EHLG------------------------LGKGDIEEDEMVSKALALSEMEASLKGSFDTQ 284

Query: 357 GVANAEGDTNSIKLDEEPSPENVSVIDAVEKTIVKDICAAENNLNKQPDLSIEDSCKTSL 416
            V +   D N I  D+               T+V  +              IE  C T+L
Sbjct: 285 RVVSGSLDVNQISEDD---------------TLVTSV-------------DIEAGCATTL 344

Query: 417 TKHDEDMSPFPDATEQVNSSLLKIDIGKHLDQTVESEGHKIGPDMLEKDNHETLIETVPS 476
             +   +S F D      S     D+G   +   E    ++G +          +ET  S
Sbjct: 345 GSNICQLSKFDDQFSSTESE--DCDVGNKTNVGEEVSSTELGDETFGN------VETENS 404

Query: 477 SGRESPLVNENPEDI-SHKNDKSPSPAAFESVDHRPVDKEDPSELSGLSSSVTCTDSAGG 536
           S     L++    ++ S KND        E++     D  + S+   +S SV    S  G
Sbjct: 405 SKMTDVLISVEKTNLESTKND-----IISEAITLVNADLSEKSQDDDVSQSVYEVQSLLG 464

Query: 537 KTQCVEVDVPEGLTSNVGSEPLYEGEECILDPGSAVLEDREPVYEGEVVLAEQGCGSSIN 596
           ++       PEG     G++ L + EE  L  G       +  + G   L          
Sbjct: 465 QSS------PEG----KGTKGLTQ-EEGELIKGFFEESVTQLTWHGLFTL---------- 489

Query: 597 FSNVQSKDEISPKQDGLKERELCVFFRNNHFSTMFKLDGQLYLLATDQGYIHQPDLVWEK 656
                        +D LKE ELCV FRNNHF+TM + + +LY L T +GY  Q DLVWE+
Sbjct: 525 -------------EDDLKEWELCVLFRNNHFATMLQHEERLYTLVTYEGYQMQQDLVWER 489

Query: 657 LNEVNGDTLFMTGNFKEFKVESHSNESWDEHNAMASTADYLASIDATKHAGLDLNS-DLQ 695
            N+++GD+ F TG F +F  +       D+ +A++ T +   SI   K   +D    D  
Sbjct: 585 FNKIDGDSAFFTGYFTKFIFK-------DQQHAISKTEN---SISRIKSENMDTTEFDRL 489