Spg003072 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg003072 vs. NCBI nr
Match: XP_023532974.1 (subtilisin-like protease SBT1.8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1296.2 bits (3353), Expect = 0.0e+00
Identity = 658/754 (87.27%), Postives = 688/754 (91.25%), Query Frame = 0

Query: 1   MIVLPSLFLLLLLDIRGGGAQVTELPLRPVLSNLQTYIVHVKKPETRANRQTDDDLENWH 60
           M+  PSLFLLLLL+    GA VTELP    L NLQTYIVHV+KPE      T DDLE+WH
Sbjct: 1   MVAFPSLFLLLLLNFHYVGALVTELP----LINLQTYIVHVEKPE------TTDDLESWH 60

Query: 61  KSFLPTSSESSEERPELLYSYRNVMSGFAARLSEEQVKAMEEKDGFVSARRERILHLHTT 120
           +SFLP+SS        LLYSYRNVMSGFAARLSEEQVKAMEEKDGFVSARRERIL LHTT
Sbjct: 61  RSFLPSSS-------SLLYSYRNVMSGFAARLSEEQVKAMEEKDGFVSARRERILQLHTT 120

Query: 121 HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPSHPSFDDAGMPPPPAKWKGRCEFNF 180
           HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGI PSHPSFDD GMPPPP KWKGRCEFNF
Sbjct: 121 HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNF 180

Query: 181 SACNNKLIGARSFNLAAKALKGETTMDDSPIDEDGHGTHTASTAAGTFVDGADALANAKG 240
           SACNNKLIGARSFNLA K LKGET MDDSPIDEDGHGTHTASTAAG FV GA+AL NAKG
Sbjct: 181 SACNNKLIGARSFNLATKVLKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKG 240

Query: 241 TAVGMAPLAHLAIYKVCFGEDCSDSDILAALDAAVEDGVDVLSLSLGGESLPFFADDIAV 300
           TAVGMAPLAHLAIYKVCFGEDC D+DILAALDAA+EDGVDVLSLSLG  S+PFF D +A+
Sbjct: 241 TAVGMAPLAHLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAI 300

Query: 301 GAFAAIQKGIFVSCSAANAGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGE 360
           GAFAAIQKGIFVSCSAAN+GPF ATLSNEAPWILTVAASTIDRRI AAAKLGNGEEFDGE
Sbjct: 301 GAFAAIQKGIFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGE 360

Query: 361 SLFQPSDFPPTFLPLVYAGEKNQTAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGVEV 420
           SLFQPSDFPPTFLPLVYAGEKNQTAALCGEGSLKDIDVKGK VVCERGGGIARIAKG EV
Sbjct: 361 SLFQPSDFPPTFLPLVYAGEKNQTAALCGEGSLKDIDVKGKIVVCERGGGIARIAKGTEV 420

Query: 421 KNAGGAAMILLNQEQDGFSTQADAHVLPATHVSHAAALKIKAYINSTTNPTATILFKGTV 480
           KNAGGAAMILLNQ+QDGFST+ADAHVLPA+HVSH AALKIKAYINSTT PTATILFKGTV
Sbjct: 421 KNAGGAAMILLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTV 480

Query: 481 IGDD-FSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISG 540
           IGDD FSPAIASFSSRGPS+ASPGILKPDITGPGVSILAAWPFPLD N+NTKSTFNIISG
Sbjct: 481 IGDDNFSPAIASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKNSNTKSTFNIISG 540

Query: 541 TSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTANITNIEGQPIPDQNLQPADLFAIGA 600
           TSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTA+ITN+EGQPI D+NLQPADLFA GA
Sbjct: 541 TSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGA 600

Query: 601 GHVNPSKAADPGLVYDIQPDDYIPYLCGLGYKNNEVGIIAHKPIDCLAKPSIPEGELNYP 660
           GHVNPSKAADPGLVYDIQPDDYIPYLCGLGYK+NEV  IA KPI+CLAKPSIPEG+LNYP
Sbjct: 601 GHVNPSKAADPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYP 660

Query: 661 SFTVALGPPQTFTRTVTNVGGGYEVYAAVVEAPPSVSVTVRPRKIFFSMMNQKVTYSVTF 720
           SFTV LGPPQTFTRTVTNVG G EVY AVVEAPPS+SVT+RP KIFFS +N+KVTYSVTF
Sbjct: 661 SFTVVLGPPQTFTRTVTNVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINEKVTYSVTF 720

Query: 721 KRIGSVSPPIEFAQGYLKWVSAKHVVRSPISVKF 754
           KRIGS+SP  EF +GYLKWVS KHVVRSPIS KF
Sbjct: 721 KRIGSISPSTEFGKGYLKWVSDKHVVRSPISFKF 737

BLAST of Spg003072 vs. NCBI nr
Match: XP_022958542.1 (subtilisin-like protease SBT1.8 [Cucurbita moschata])

HSP 1 Score: 1292.3 bits (3343), Expect = 0.0e+00
Identity = 658/754 (87.27%), Postives = 685/754 (90.85%), Query Frame = 0

Query: 1   MIVLPSLFLLLLLDIRGGGAQVTELPLRPVLSNLQTYIVHVKKPETRANRQTDDDLENWH 60
           M+  PSLFLLLLL+    GA VTELP    L NLQTYIVHVKKPE      T DDLE WH
Sbjct: 1   MVAFPSLFLLLLLNFHYVGALVTELP----LINLQTYIVHVKKPE------TTDDLEIWH 60

Query: 61  KSFLPTSSESSEERPELLYSYRNVMSGFAARLSEEQVKAMEEKDGFVSARRERILHLHTT 120
           +SFLP+SS        LLYSYRNVMSGFAARLSEEQVKAMEE DGFVSARRERIL LHTT
Sbjct: 61  RSFLPSSS-------SLLYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTT 120

Query: 121 HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPSHPSFDDAGMPPPPAKWKGRCEFNF 180
           HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGI PSHPSFDD GMPPPP KWKGRCEFNF
Sbjct: 121 HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNF 180

Query: 181 SACNNKLIGARSFNLAAKALKGETTMDDSPIDEDGHGTHTASTAAGTFVDGADALANAKG 240
           SACNNKLIGARSFNLA KALKGET MDDSPIDEDGHGTHTASTAAG FV GA+AL NAKG
Sbjct: 181 SACNNKLIGARSFNLATKALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKG 240

Query: 241 TAVGMAPLAHLAIYKVCFGEDCSDSDILAALDAAVEDGVDVLSLSLGGESLPFFADDIAV 300
           TAVGMAPLAHLAIYKVCFGEDC D+DILAALDAA+EDGVDVLSLSLG  S+PFF D +A+
Sbjct: 241 TAVGMAPLAHLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAI 300

Query: 301 GAFAAIQKGIFVSCSAANAGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGE 360
           GAFAAIQKGIFVSCSAAN+GPF ATLSNEAPWILTVAASTIDRRI AAAKLGNGEEFDGE
Sbjct: 301 GAFAAIQKGIFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGE 360

Query: 361 SLFQPSDFPPTFLPLVYAGEKNQTAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGVEV 420
           SLFQPSDFPPT LPLVYAGEKNQTAALCGEGSLKDIDVKGK VVCERGGGIARIAKG EV
Sbjct: 361 SLFQPSDFPPTLLPLVYAGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEV 420

Query: 421 KNAGGAAMILLNQEQDGFSTQADAHVLPATHVSHAAALKIKAYINSTTNPTATILFKGTV 480
           KNAGGAAMILLNQ+QDGFST+ADAHVLPA+HVSH AALKIKAYINSTT PTATILFKGTV
Sbjct: 421 KNAGGAAMILLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTV 480

Query: 481 IGDD-FSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISG 540
           IGDD FSPAIASFSSRGPS+ASPGILKPDITGPGVSILAAWPFPLD N NTKSTFNIISG
Sbjct: 481 IGDDNFSPAIASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISG 540

Query: 541 TSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTANITNIEGQPIPDQNLQPADLFAIGA 600
           TSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTA+ITN+EGQPI D+NLQPADLFA GA
Sbjct: 541 TSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGA 600

Query: 601 GHVNPSKAADPGLVYDIQPDDYIPYLCGLGYKNNEVGIIAHKPIDCLAKPSIPEGELNYP 660
           GHVNPSKAADPGLVYDIQPDDYIPYLCGLGYK+NEV  IA KPI+CLAKPSIPEG+LNYP
Sbjct: 601 GHVNPSKAADPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYP 660

Query: 661 SFTVALGPPQTFTRTVTNVGGGYEVYAAVVEAPPSVSVTVRPRKIFFSMMNQKVTYSVTF 720
           SFTV LGPPQTFTRTVTNVG G EVY AVVEAPPS+SVT+RP KIFFS +NQKVT+SVTF
Sbjct: 661 SFTVVLGPPQTFTRTVTNVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTF 720

Query: 721 KRIGSVSPPIEFAQGYLKWVSAKHVVRSPISVKF 754
           KRIGS+SP  EF +GYLKWVS KHVVRSPIS KF
Sbjct: 721 KRIGSISPSTEFGKGYLKWVSDKHVVRSPISFKF 737

BLAST of Spg003072 vs. NCBI nr
Match: KAG6606138.1 (Subtilisin-like protease 1.8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1289.6 bits (3336), Expect = 0.0e+00
Identity = 657/754 (87.14%), Postives = 684/754 (90.72%), Query Frame = 0

Query: 1   MIVLPSLFLLLLLDIRGGGAQVTELPLRPVLSNLQTYIVHVKKPETRANRQTDDDLENWH 60
           M+  PSLFLLLLLD    GA VTELP    L NLQTYIVHVKKPE      T DDLE+WH
Sbjct: 1   MVAFPSLFLLLLLDFHYVGALVTELP----LINLQTYIVHVKKPE------TTDDLESWH 60

Query: 61  KSFLPTSSESSEERPELLYSYRNVMSGFAARLSEEQVKAMEEKDGFVSARRERILHLHTT 120
           +SFLP+SS        LLYSYRNVMSGFAARLSEEQVKAMEE DGFVSARRERIL LHTT
Sbjct: 61  RSFLPSSS-------SLLYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTT 120

Query: 121 HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPSHPSFDDAGMPPPPAKWKGRCEFNF 180
           HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGI PSHPSFDD GMPPPP KWKGRCEFNF
Sbjct: 121 HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNF 180

Query: 181 SACNNKLIGARSFNLAAKALKGETTMDDSPIDEDGHGTHTASTAAGTFVDGADALANAKG 240
           SACNNKLIGARSFNLA KALKGET MDDSPIDEDGHGTHTASTAAG FV GA+AL NAKG
Sbjct: 181 SACNNKLIGARSFNLATKALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKG 240

Query: 241 TAVGMAPLAHLAIYKVCFGEDCSDSDILAALDAAVEDGVDVLSLSLGGESLPFFADDIAV 300
           TAVGMAPLAHLAIYKVCFGEDC D+DILAALDAA+EDGVDVLSLSLG  S+PFF D +A+
Sbjct: 241 TAVGMAPLAHLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAI 300

Query: 301 GAFAAIQKGIFVSCSAANAGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGE 360
           GAFAAIQKGIFVSCSAAN+GPF ATLSNEAPWILTVAASTIDRRI AAAKLGNGEE DGE
Sbjct: 301 GAFAAIQKGIFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEECDGE 360

Query: 361 SLFQPSDFPPTFLPLVYAGEKNQTAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGVEV 420
           SLFQPSDFPPT LPLVYAGEKNQTAALCGEGSLKDIDVKGK VVCERGGGIARIAKG EV
Sbjct: 361 SLFQPSDFPPTLLPLVYAGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEV 420

Query: 421 KNAGGAAMILLNQEQDGFSTQADAHVLPATHVSHAAALKIKAYINSTTNPTATILFKGTV 480
           KNAGGAAMILLNQ+QDGFST+ADAHVLPA+HVSH AALKIKAYINSTT PTATILFKGTV
Sbjct: 421 KNAGGAAMILLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTV 480

Query: 481 IGDD-FSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISG 540
           IGDD FSPAIASFSSRGPS+ASPGILKPDITGPGVSILAAWPFPLD N NTKSTFNIISG
Sbjct: 481 IGDDNFSPAIASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISG 540

Query: 541 TSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTANITNIEGQPIPDQNLQPADLFAIGA 600
           TSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTA+ITN+EGQPI D+NLQPADLFA GA
Sbjct: 541 TSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGA 600

Query: 601 GHVNPSKAADPGLVYDIQPDDYIPYLCGLGYKNNEVGIIAHKPIDCLAKPSIPEGELNYP 660
           GHVNPSKAADPGLVYDIQPDDYIPYLCGLGYK+NEV  IA KPI+CLAKPSIPEG+LNYP
Sbjct: 601 GHVNPSKAADPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYP 660

Query: 661 SFTVALGPPQTFTRTVTNVGGGYEVYAAVVEAPPSVSVTVRPRKIFFSMMNQKVTYSVTF 720
           SFTV LGPPQTFTRTVTNVG G EVY AVVEA PS+SVT+RP KIFFS +NQKVT+SVTF
Sbjct: 661 SFTVVLGPPQTFTRTVTNVGCGREVYTAVVEASPSISVTIRPSKIFFSKINQKVTFSVTF 720

Query: 721 KRIGSVSPPIEFAQGYLKWVSAKHVVRSPISVKF 754
           KRIGS+SP  EF +GYLKWVS KHVVRSPIS KF
Sbjct: 721 KRIGSISPSTEFGKGYLKWVSDKHVVRSPISFKF 737

BLAST of Spg003072 vs. NCBI nr
Match: KAG7036083.1 (Subtilisin-like protease SBT1.8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1289.2 bits (3335), Expect = 0.0e+00
Identity = 657/754 (87.14%), Postives = 684/754 (90.72%), Query Frame = 0

Query: 1   MIVLPSLFLLLLLDIRGGGAQVTELPLRPVLSNLQTYIVHVKKPETRANRQTDDDLENWH 60
           M+  PSLFLLLLL+    GA VTELP    L NLQTYIVHVKKPE      T DDLE WH
Sbjct: 65  MVAFPSLFLLLLLNFHYVGALVTELP----LINLQTYIVHVKKPE------TTDDLEIWH 124

Query: 61  KSFLPTSSESSEERPELLYSYRNVMSGFAARLSEEQVKAMEEKDGFVSARRERILHLHTT 120
           +SFLP+SS        LLYSYRNVMSGFAARLSEEQVKAMEE DGFVSARRERIL LHTT
Sbjct: 125 RSFLPSSS-------SLLYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTT 184

Query: 121 HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPSHPSFDDAGMPPPPAKWKGRCEFNF 180
           HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGI PSHPSFDD GMPPPP KWKGRCEFNF
Sbjct: 185 HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNF 244

Query: 181 SACNNKLIGARSFNLAAKALKGETTMDDSPIDEDGHGTHTASTAAGTFVDGADALANAKG 240
           SACNNKLIGARSFNLA KALKGET MDDSPIDEDGHGTHTASTAAG FV GA+AL NAKG
Sbjct: 245 SACNNKLIGARSFNLATKALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKG 304

Query: 241 TAVGMAPLAHLAIYKVCFGEDCSDSDILAALDAAVEDGVDVLSLSLGGESLPFFADDIAV 300
           TAVGMAPLAHLAIYKVCFGEDC D+DILAALDAA+EDGVDVLSLSLG  S+PFF D +A+
Sbjct: 305 TAVGMAPLAHLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAI 364

Query: 301 GAFAAIQKGIFVSCSAANAGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGE 360
           GAFAAIQKGIFVSCSAAN+GPF ATLSNEAPWILTVAASTIDRRI AAAKLGNGEEFDGE
Sbjct: 365 GAFAAIQKGIFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGE 424

Query: 361 SLFQPSDFPPTFLPLVYAGEKNQTAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGVEV 420
           SLFQPSDFPPT LPLVYAGEKNQTAALCGEGSLKDIDVKGK VVCERGGGIARIAKG EV
Sbjct: 425 SLFQPSDFPPTLLPLVYAGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEV 484

Query: 421 KNAGGAAMILLNQEQDGFSTQADAHVLPATHVSHAAALKIKAYINSTTNPTATILFKGTV 480
           KNAGGAAMILLNQ+QDGFST+ADAHVLPA+HVSH AALKIKAYINSTT PTATILFKGTV
Sbjct: 485 KNAGGAAMILLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTV 544

Query: 481 IGDD-FSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISG 540
           IGDD FSPAIASFSSRGPS+ASPGILKPDITGPGVSILAAWPFPLD N NTKSTFNIISG
Sbjct: 545 IGDDNFSPAIASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISG 604

Query: 541 TSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTANITNIEGQPIPDQNLQPADLFAIGA 600
           TSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTA+ITN+EGQPI D+NLQPADLFA GA
Sbjct: 605 TSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGA 664

Query: 601 GHVNPSKAADPGLVYDIQPDDYIPYLCGLGYKNNEVGIIAHKPIDCLAKPSIPEGELNYP 660
           GHVNPSKAADPGLVYDIQPDDYIPYLCGLGYK+NEV  IA KPI+CLAKPSIPEG+LNYP
Sbjct: 665 GHVNPSKAADPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYP 724

Query: 661 SFTVALGPPQTFTRTVTNVGGGYEVYAAVVEAPPSVSVTVRPRKIFFSMMNQKVTYSVTF 720
           SFTV LGPPQTFTRTVTNVG G EVY AVVEA PS+SVT+RP KIFFS +NQKVT+SVTF
Sbjct: 725 SFTVVLGPPQTFTRTVTNVGCGREVYTAVVEASPSISVTIRPSKIFFSKINQKVTFSVTF 784

Query: 721 KRIGSVSPPIEFAQGYLKWVSAKHVVRSPISVKF 754
           KRIGS+SP  EF +GYLKWVS KHVVRSPIS KF
Sbjct: 785 KRIGSISPSTEFGKGYLKWVSDKHVVRSPISFKF 801

BLAST of Spg003072 vs. NCBI nr
Match: KAA0042818.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])

HSP 1 Score: 1274.6 bits (3297), Expect = 0.0e+00
Identity = 642/755 (85.03%), Postives = 684/755 (90.60%), Query Frame = 0

Query: 1   MIVLPSLFLLLLLDIRGGGAQVTELPLRPVLSNLQTYIVHVKKPETRANRQTDDDLENWH 60
           M++LPSLFLLLLL+  G  AQVTELP    LSNL TYIVHVKKPE        DDLE WH
Sbjct: 1   MVLLPSLFLLLLLNFHGYEAQVTELP----LSNLHTYIVHVKKPE------VVDDLEIWH 60

Query: 61  KSFLPTSSESSEERPELLYSYRNVMSGFAARLSEEQVKAMEEKDGFVSARRERILHLHTT 120
           +SFLPTS ++ EE+P LLYSYRNVMSGF+ARL+EE VKAMEEKDGFVSARRE I+HLHTT
Sbjct: 61  RSFLPTSLDNEEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTT 120

Query: 121 HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPSHPSFDDAGMPPPPAKWKGRCEFNF 180
           HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITP+HPSFDDAGMP PPAKWKGRCEFNF
Sbjct: 121 HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEFNF 180

Query: 181 SACNNKLIGARSFNLAAKALKGE-TTMDDSPIDEDGHGTHTASTAAGTFVDGADALANAK 240
           SACNNKLIGARS NLA++ALKG+ TT+DDSPIDEDGHGTHTASTAAGTFVDGA+AL NA 
Sbjct: 181 SACNNKLIGARSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAF 240

Query: 241 GTAVGMAPLAHLAIYKVCFGEDCSDSDILAALDAAVEDGVDVLSLSLGGESLPFFADDIA 300
           GTAVGMAPLAHLAIYKVCFGEDCSD DILA LDAAVEDGVDVLS+SLGG S+PFFAD  A
Sbjct: 241 GTAVGMAPLAHLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITA 300

Query: 301 VGAFAAIQKGIFVSCSAANAGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDG 360
           +G+FAAIQKGIFVSCSAAN+GPFNATLSNEAPWILTVAASTIDR+I A AKLGNGEEFDG
Sbjct: 301 IGSFAAIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDG 360

Query: 361 ESLFQPSDFPPTFLPLVYAGEKNQTAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGVE 420
           ESLFQP+DFP T LPLV+ GEKN+T ALC EGSLK+IDVKGK VVCERGGGIARIAKGVE
Sbjct: 361 ESLFQPNDFPQTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVE 420

Query: 421 VKNAGGAAMILLNQEQDGFSTQADAHVLPATHVSHAAALKIKAYINSTTNPTATILFKGT 480
           VKN GGAAMILLN E DGF+T+ DAHVLPA+HVSH AALKIKAYINSTT PTATILFKGT
Sbjct: 421 VKNGGGAAMILLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGT 480

Query: 481 VIGDDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISG 540
            IGDDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISG
Sbjct: 481 TIGDDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISG 540

Query: 541 TSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTANITNIEGQPIPDQNLQPADLFAIGA 600
           TSMSCPHLSGIAALIKS+HPDWSPAAIKS+IMTTANITN+EG PI D+ LQPADLFAIGA
Sbjct: 541 TSMSCPHLSGIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGA 600

Query: 601 GHVNPSKAADPGLVYDIQPDDYIPYLCGLGYKNNEVGIIAHKPIDCLAKPSIPEGELNYP 660
           GHVNPSKA DPGLVYDIQPDDYIPYLCGLGY NN+V +IAHKPIDCL   SIPEGELNYP
Sbjct: 601 GHVNPSKAIDPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYP 660

Query: 661 SFTVALGPPQTFTRTVTNVGGGYEVYAAVVEAPPSVSVTVRPRKIFFSMMNQKVTYSVTF 720
           SF V LGP QTF+RTVT+VG G  VY  V+EAP  VSVTVRPRK+ FS +NQK TYSVTF
Sbjct: 661 SFMVKLGPVQTFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTF 720

Query: 721 KRIGSVSPPIEFAQGYLKWVSAKHVVRSPISVKFV 755
           KRIGS+SP IEFA+GYLKWVSAKHVVRSPISVKFV
Sbjct: 721 KRIGSISPSIEFAEGYLKWVSAKHVVRSPISVKFV 745

BLAST of Spg003072 vs. ExPASy Swiss-Prot
Match: A9QY38 (Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1)

HSP 1 Score: 999.6 bits (2583), Expect = 1.9e-290
Identity = 507/752 (67.42%), Postives = 596/752 (79.26%), Query Frame = 0

Query: 9   LLLLLDIRGGGAQVTELPLRPV----LSNLQTYIVHVKKPETRANRQTDDDLENWHKSFL 68
           L  LL      AQ +ELP         S+ + YI+HV  PE +   ++ +DLE+W+ SFL
Sbjct: 9   LTFLLTFHVHNAQGSELPTTTTESTETSSSKIYIIHVTGPEGKMLTES-EDLESWYHSFL 68

Query: 69  PTSSESSEERPELLYSYRNVMSGFAARLSEEQVKAMEEKDGFVSARRERILHLHTTHTPD 128
           P +  SSEE+P ++YSY+NV+ GFAA L++E++ A+E+K+GF+SA  +R+LH  TTHTP 
Sbjct: 69  PPTLMSSEEQPRVIYSYKNVLRGFAASLTQEELSAVEKKNGFISAHPQRVLHRQTTHTPK 128

Query: 129 FLGLNRQFGFWKDSNFGKGVIIGVLDGGITPSHPSFDDAGMPPPPAKWKGRCEFNFSACN 188
           FLGL +  G WK+SNFGKGVIIGVLD GITP HPSF D G+PPPP KWKGRC+ N +ACN
Sbjct: 129 FLGLQQDTGVWKESNFGKGVIIGVLDSGITPGHPSFSDVGIPPPPPKWKGRCDLNVTACN 188

Query: 189 NKLIGARSFNLAAKALKGETTMDDSPIDEDGHGTHTASTAAGTFVDGADALANAKGTAVG 248
           NKLIGAR+FNLAA+A+ G+    ++PIDEDGHGTHTASTAAG FV+ A+ L NAKGTA G
Sbjct: 189 NKLIGARAFNLAAEAMNGKKA--EAPIDEDGHGTHTASTAAGAFVNYAEVLGNAKGTAAG 248

Query: 249 MAPLAHLAIYKVCFGEDCSDSDILAALDAAVEDGVDVLSLSLG-GESLPFFADDIAVGAF 308
           MAP AHLAIYKVCFGEDC +SDILAALDAAVEDGVDV+S+SLG  E  PFF D  A+GAF
Sbjct: 249 MAPHAHLAIYKVCFGEDCPESDILAALDAAVEDGVDVISISLGLSEPPPFFNDSTAIGAF 308

Query: 309 AAIQKGIFVSCSAANAGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLF 368
           AA+QKGIFVSC+A N+GPFN+++ N APWILTV ASTIDRRIVA AKLGNG+EFDGES+F
Sbjct: 309 AAMQKGIFVSCAAGNSGPFNSSIVNAAPWILTVGASTIDRRIVATAKLGNGQEFDGESVF 368

Query: 369 QPSDFPPTFLPLVYAGEK-NQTAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGVEVKN 428
           QPS F PT LPL YAG+   + +A C  GSL D   +GK V+CERGGGIARIAKG EVK 
Sbjct: 369 QPSSFTPTLLPLAYAGKNGKEESAFCANGSLDDSAFRGKVVLCERGGGIARIAKGEEVKR 428

Query: 429 AGGAAMILLNQEQDGFSTQADAHVLPATHVSHAAALKIKAYINSTTNPTATILFKGTVIG 488
           AGGAAMIL+N E + FS  AD H LPATHVS+AA ++IKAYINST  PTATILFKGTVIG
Sbjct: 429 AGGAAMILMNDETNAFSLSADVHALPATHVSYAAGIEIKAYINSTATPTATILFKGTVIG 488

Query: 489 DDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSM 548
           +  +PA+ASFSSRGP+L SPGILKPDI GPGV+ILAAWPFPL N+T++K TFNI SGTSM
Sbjct: 489 NSLAPAVASFSSRGPNLPSPGILKPDIIGPGVNILAAWPFPLSNSTDSKLTFNIESGTSM 548

Query: 549 SCPHLSGIAALIKSSHPDWSPAAIKSAIMTTANITNIEGQPIPDQNLQPADLFAIGAGHV 608
           SCPHLSGIAAL+KSSHP WSPAAIKSAIMT+A+  N+  + I D+ LQP DLFA G+GHV
Sbjct: 549 SCPHLSGIAALLKSSHPHWSPAAIKSAIMTSADTINLGNKLIVDETLQPTDLFATGSGHV 608

Query: 609 NPSKAADPGLVYDIQPDDYIPYLCGLGYKNNEVGIIAHKPIDCLAKPSIPEGELNYPSFT 668
           NPS+A DPGLVYDIQPDDYIPYLCGLGY   EVGIIAH+ I C A  SIPEGELNYPSF+
Sbjct: 609 NPSRANDPGLVYDIQPDDYIPYLCGLGYSETEVGIIAHRKIKCSA--SIPEGELNYPSFS 668

Query: 669 VALGPPQTFTRTVTNVGGGYEVYAAVVEAPPSVSVTVRPRKIFFSMMNQKVTYSVTFKRI 728
           V LG  +TFTRTVTNVG  +  Y  +V AP  V V V+P K+ FS +NQK TYSVTF R 
Sbjct: 669 VELGSSKTFTRTVTNVGEAHSSYDLIVAAPQGVDVKVQPYKLNFSEVNQKETYSVTFSRT 728

Query: 729 GSVSPPIEFAQGYLKWVSAKHVVRSPISVKFV 755
           G  +   E+AQG+LKWVS KH VRSPISVKF+
Sbjct: 729 GLGNKTQEYAQGFLKWVSTKHTVRSPISVKFI 755

BLAST of Spg003072 vs. ExPASy Swiss-Prot
Match: A0A0M3R8G2 (Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1)

HSP 1 Score: 882.5 bits (2279), Expect = 3.4e-255
Identity = 450/723 (62.24%), Postives = 549/723 (75.93%), Query Frame = 0

Query: 32  SNLQTYIVHVKKPETRANRQTDDDLENWHKSFLPTSSESSEERPELLYSYRNVMSGFAAR 91
           + LQTYIVHV KP+ +       DLE+++ SFLP +   SE    +++SY +V +GFAA+
Sbjct: 24  TGLQTYIVHVDKPDAQV-LANSADLESYYNSFLPATVSGSEVPSRIIHSYHHVATGFAAK 83

Query: 92  LSEEQVKAMEEKDGFVSARRERILHLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDG 151
           LS E+VK ME+K GFVSA+ E++L LHTTHTP+FLGL +  GFW++SN+GKGVIIG+LD 
Sbjct: 84  LSGEEVKEMEKKAGFVSAKLEKVLTLHTTHTPNFLGLYQNMGFWQESNYGKGVIIGLLDT 143

Query: 152 GITPSHPSFDDAGMPPPPAKWKGRCEFNFSA-CNNKLIGARSFNLAAKALKGETTMDDSP 211
           GITP HPSF D  MP PPAKWKG+CEF  +A CN K+IGAR+F      + G       P
Sbjct: 144 GITPGHPSFSDVNMPSPPAKWKGKCEFTGNATCNKKIIGARNF------ISGSGV---PP 203

Query: 212 IDEDGHGTHTASTAAGTFVDGADALANAKGTAVGMAPLAHLAIYKVCFGEDCSDSDILAA 271
            DE+GHGTHTASTAAG FV+ A+   NA GTAVGMAPLAH+A+YKVC  + CSD+DILAA
Sbjct: 204 TDEEGHGTHTASTAAGNFVNDANVFGNANGTAVGMAPLAHIAMYKVCSEDGCSDADILAA 263

Query: 272 LDAAVEDGVDVLSLSLGGESLPFFADDIAVGAFAAIQKGIFVSCSAANAGPFNATLSNEA 331
           LDAA++DGVDVLSLSLGG S PF+ D+IA+GAFAAI+KGIFVS SA N GP N+TLSNEA
Sbjct: 264 LDAAIDDGVDVLSLSLGGYSDPFYYDNIAIGAFAAIRKGIFVSASAGNDGPLNSTLSNEA 323

Query: 332 PWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQPSDFPPTFLPLVYAGEKNQTAALCGE 391
           PWILTV AST DR+IVA A LGNG+++DGES FQP+DFP T LPLVY G  ++ AA C  
Sbjct: 324 PWILTVGASTHDRKIVATAVLGNGQQYDGESAFQPADFPHTLLPLVYPGTSDEEAAFCSS 383

Query: 392 GSLKDIDVKGKAVVCERGGGIARIAKGVEVKNAGGAAMILLNQEQDGFSTQADAHVLPAT 451
           GSL   DVKGK VVC+RGG +AR+ K   VK+AGGAAMIL N E DG  T ADAHVLPAT
Sbjct: 384 GSLDKFDVKGKVVVCDRGGDVARLEKSQTVKDAGGAAMILANLEIDGEGTFADAHVLPAT 443

Query: 452 HVSHAAALKIKAYINSTTNPTATILFKGTVIGDDFSPAIASFSSRGPSLASPGILKPDIT 511
           HV +AA   IK+YINST+ PTA ILFKGT+IG   SP+++SFSSRGP+LASPGI+KPDI 
Sbjct: 444 HVGYAAGEMIKSYINSTSTPTAGILFKGTIIGFKSSPSVSSFSSRGPNLASPGIVKPDII 503

Query: 512 GPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSGIAALIKSSHPDWSPAAIKSAI 571
           GPGV+ILAAWP  ++N T T  TFNIISGTSMSCPHLSGI AL+KS+HPDWSPAAIKSAI
Sbjct: 504 GPGVNILAAWPVSVENKTGTDLTFNIISGTSMSCPHLSGIVALLKSAHPDWSPAAIKSAI 563

Query: 572 MTTANITNIEGQPIPDQNLQPADLFAIGAGHVNPSKAADPGLVYDIQPDDYIPYLCGLGY 631
           MT+A+ +N+EGQPI D+   PAD+FA GAGHVNPSKA+DPGL+YDIQ +DYI YLCGLGY
Sbjct: 564 MTSADQSNLEGQPILDERNLPADIFATGAGHVNPSKASDPGLIYDIQLEDYIQYLCGLGY 623

Query: 632 KNNEVGIIAHKPIDCLAKPSIPEGELNYPSFTVALGP-PQTFTRTVTNVGGGYEVYAAVV 691
           +  ++G+I  + + C  + SI E ELNYPSF++ LGP  Q +TRTVTNVG     Y   +
Sbjct: 624 REKDIGLIVQETVKC--ESSISEAELNYPSFSIILGPKTQNYTRTVTNVGDASSTYTVNI 683

Query: 692 EAPPSVSVTVRPRKIFFSMMNQKVTYSVTFKRIGSVSPPIEFAQGYLKWVSAKHVVRSPI 751
                V + V P  + F+ M Q+ TY+V+F + G  +    F QG + W S ++VVRSPI
Sbjct: 684 AKTQGVDIVVEPATLVFTKMYQQATYTVSFTQSGDFTD--RFVQGAISWSSNEYVVRSPI 732

Query: 752 SVK 753
           SVK
Sbjct: 744 SVK 732

BLAST of Spg003072 vs. ExPASy Swiss-Prot
Match: A9QY40 (Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1)

HSP 1 Score: 859.8 bits (2220), Expect = 2.4e-248
Identity = 453/728 (62.23%), Postives = 541/728 (74.31%), Query Frame = 0

Query: 33  NLQTYIVHVKKPETRANRQTDDDLENWHKSFLPTSSESSEERPELLYSYRNVMSGFAARL 92
           NL TYIVHVKK E     Q+ ++L  WH SFLP +S     +  +++SYRNV SGFA RL
Sbjct: 37  NLTTYIVHVKKLEIEGPLQSTEELHTWHHSFLPETS----NKDRMVFSYRNVASGFAVRL 96

Query: 93  SEEQVKAMEEKDGFVSARRERILHLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGG 152
           + E+  A++EK+  +S R ER L LHTTHTP FLGL +  G W DSN GKGVIIGV+D G
Sbjct: 97  TPEEANALQEKEEVMSIRPERTLSLHTTHTPSFLGLRQGQGLWNDSNLGKGVIIGVIDTG 156

Query: 153 ITPSHPSFDDAGMPPPPAKWKGRCEF-NFSACNNKLIGARSFNLAAKALKGETTMDDSPI 212
           I P H SF+D GMPPPPAKWKG CEF   S CNNKLIGAR  NL   A++      + P 
Sbjct: 157 IYPFHLSFNDEGMPPPPAKWKGHCEFTGGSVCNNKLIGAR--NLVKSAIQ------EPPY 216

Query: 213 DEDGHGTHTASTAAGTFVDGADALANAKGTAVGMAPLAHLAIYKVCFG---EDCSDSDIL 272
           ++  HGTHTA+ AAG FV+GA    NA+GTA GMAP AHLAIYKVC     ++C +S IL
Sbjct: 217 EDFFHGTHTAAEAAGRFVEGASVFGNARGTAAGMAPDAHLAIYKVCSSKVKDECPESAIL 276

Query: 273 AALDAAVEDGVDVLSLSLGGESLPFFADDIAVGAFAAIQKGIFVSCSAANAGPFNATLSN 332
           AA+D A+EDGVDVLSLSLG  SLPFF D IA+GAFAA QKGIFVSCSAAN+GP  ++LSN
Sbjct: 277 AAMDIAIEDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQKGIFVSCSAANSGPHYSSLSN 336

Query: 333 EAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQPSDFPPTFLPLVY-AGEKNQTAAL 392
           EAPWILTV ASTIDR+I A+AKLGNG E++GE+LFQP DF    LPLVY A EKN ++AL
Sbjct: 337 EAPWILTVGASTIDRKISASAKLGNGAEYEGETLFQPKDFSSQLLPLVYAAAEKNNSSAL 396

Query: 393 CGEGSLKDIDVKGKAVVCERGGGIARIAKGVEVKNAGGAAMILLNQEQDGFSTQADAHVL 452
           C  GSL++I+VKGK VVC+ GGGI  IAKG EV +AGG+AMIL N E  GF+T A+AHVL
Sbjct: 397 CAPGSLRNINVKGKVVVCDLGGGIPFIAKGQEVLDAGGSAMILANIENFGFTTLANAHVL 456

Query: 453 PATHVSHAAALKIKAYINSTTNPTATILFKGTVIGDDFSPAIASFSSRGPSLASPGILKP 512
           PA HVS+AA+L IKAYINST  PTAT+LF+GT+IGD  +P++A+FSSRGPS  SPGILKP
Sbjct: 457 PAVHVSYAASLAIKAYINSTYTPTATVLFQGTIIGDSLAPSVAAFSSRGPSQQSPGILKP 516

Query: 513 DITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSGIAALIKSSHPDWSPAAIK 572
           DI GPGV+ILAAW   +DN       F+IISGTSMSCPHLSGIAAL+KS+HPDWSPAAIK
Sbjct: 517 DIIGPGVNILAAWAVSVDNKI---PAFDIISGTSMSCPHLSGIAALLKSAHPDWSPAAIK 576

Query: 573 SAIMTTANITNIEGQPIPDQNLQPADLFAIGAGHVNPSKAADPGLVYDIQPDDYIPYLCG 632
           SAIMTTAN  N+ G PI DQ LQPAD+FA GAGHVNP +A DPGLVYDIQP+DY+PYLCG
Sbjct: 577 SAIMTTANTLNLRGLPILDQRLQPADIFATGAGHVNPVRANDPGLVYDIQPEDYVPYLCG 636

Query: 633 LGYKNNEVGIIAHKPIDCLAKPSIPEGELNYPSFTVALG-PPQTFTRTVTNVGGGYEVYA 692
           LGY + EV II  + + C    SI + ELNYPSF++ LG   Q +TRT+TNVG     Y 
Sbjct: 637 LGYSDREVTIIVQRSVRCFNVKSIAQAELNYPSFSILLGSDSQFYTRTLTNVGPANSTYT 696

Query: 693 AVVEAPPSVSVTVRPRKIFFSMMNQKVTYSVTF-KRIGSVSPPIEFAQGYLKWVSAKHVV 752
             ++ P ++ ++V P +I F+ +NQKV Y V F  +I        FAQG + WVS KHVV
Sbjct: 697 VKIDVPLAMGISVSPSQITFTQVNQKVAYFVDFIPQIKENRGNHTFAQGAITWVSDKHVV 749

Query: 753 RSPISVKF 754
           R+PISV F
Sbjct: 757 RTPISVIF 749

BLAST of Spg003072 vs. ExPASy Swiss-Prot
Match: A9QY39 (Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1)

HSP 1 Score: 823.5 bits (2126), Expect = 1.9e-237
Identity = 443/759 (58.37%), Postives = 543/759 (71.54%), Query Frame = 0

Query: 3   VLPSLFLLLLLDIRGGGAQ---VTELPLRPVLSNLQTYIVHVKKPETRANRQTDDDLENW 62
           +L +L L+L + +    +Q    TEL      SNL TYIVHV+KP+   +    DDL  +
Sbjct: 9   LLLALILVLSVSLASAASQNLEFTELEDED-QSNLSTYIVHVRKPQVIQS----DDLHTF 68

Query: 63  HKSFLPTSSESSEERPELLYSYRNVMSGFAARLSEEQVKAMEEKDGFVSARRERILHLHT 122
           + S LP S++++ +R  ++++YRNV++GFA +L+ E+ KA+++ +  VSAR E+IL LHT
Sbjct: 69  YYSLLPESTKTTNQR--IVFTYRNVVNGFAVKLTPEEAKALQQNEEVVSARPEKILSLHT 128

Query: 123 THTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPSHPSFDDAGMPPPPAKWKGRCEFN 182
           THTP FLGL +  G WK SN GKGVIIG+LD GI+P HPSF D GMP PPAKW G CEF 
Sbjct: 129 THTPSFLGLQQGLGLWKGSNSGKGVIIGILDTGISPFHPSFSDEGMPSPPAKWNGICEFT 188

Query: 183 FS-ACNNKLIGARSFNLAAKALKGETTMDDSPIDEDGHGTHTASTAAGTFVDGADALANA 242
               CNNK+IGAR+F         +T     P D+ GHGTHTASTAAG  V GA+   NA
Sbjct: 189 GKRTCNNKIIGARNF--------VKTKNLTLPFDDVGHGTHTASTAAGRPVQGANVYGNA 248

Query: 243 KGTAVGMAPLAHLAIYKVCFGEDCSDSDILAALDAAVEDGVDVLSLSLGGESLPFFADDI 302
            GTAVGMAP AH+A+YKVC    CS+S ILA +D AV+DGVDVLSLSLGG S PFF D I
Sbjct: 249 NGTAVGMAPDAHIAMYKVCGLVGCSESAILAGMDTAVDDGVDVLSLSLGGPSGPFFEDPI 308

Query: 303 AVGAFAAIQKGIFVSCSAANAGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFD 362
           A+GAF AIQKGIFVSCSAAN+GP  ++LSNEAPWILTV AS+IDR I+A AKLGNG+E+ 
Sbjct: 309 ALGAFGAIQKGIFVSCSAANSGPAYSSLSNEAPWILTVGASSIDRTIMATAKLGNGKEYV 368

Query: 363 GESLFQPSDFPPTFLPLVYAGE--KNQTAALCGEGSLKDIDVKGKAVVCERGGGIARIAK 422
           G+S+FQP DF P+ LPLVYAG    N  +  C   SL   DV+GK V+CE GG + R+ K
Sbjct: 369 GQSVFQPKDFAPSLLPLVYAGANGNNNFSVFCAPESLNRSDVEGKVVLCEDGGFVPRVFK 428

Query: 423 GVEVKNAGGAAMILLNQEQDGFSTQADAHVLPATHVSHAAALKIKAYINSTTNPTATILF 482
           G  VK+AGGAAMIL+N   + F+  AD HVLPA H+S+ A L +K YINST+ PTATILF
Sbjct: 429 GKAVKDAGGAAMILMNSVLEDFNPIADVHVLPAVHISYEAGLALKEYINSTSTPTATILF 488

Query: 483 KGTVIGDDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNI 542
           +GTVIG+  +P + SFSSRGPS ASPGILKPDI GPG++ILAAWP  LDN+T     FNI
Sbjct: 489 EGTVIGNLLAPQVTSFSSRGPSKASPGILKPDIIGPGLNILAAWPVSLDNSTT--PPFNI 548

Query: 543 ISGTSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTANITNIEGQPIPDQNLQPADLFA 602
           ISGTSMSCPHLSGIAAL+K+SHPDWSPAAIKSAIMTTA+  N+ G PI DQ L PAD+FA
Sbjct: 549 ISGTSMSCPHLSGIAALLKNSHPDWSPAAIKSAIMTTASQVNLGGTPILDQRLVPADVFA 608

Query: 603 IGAGHVNPSKAADPGLVYDIQPDDYIPYLCGLGYKNNEVGIIAHKPIDCLAKPSIPEGEL 662
            GAGHVNP KA DPGLVYDI+P+DYIPYLCGL Y + EVG+I  + + C     I E EL
Sbjct: 609 TGAGHVNPVKANDPGLVYDIEPNDYIPYLCGLNYTDREVGVILQQRVRCSEVNHIAEAEL 668

Query: 663 NYPSFTVALG-PPQTFTRTVTNVGGGYEVYAAVVEAPPSVSVTVRPRKIFFSMMNQKVTY 722
           NYPSF++ LG   Q +TRTV NVG     Y A +  P  V +++ P ++ F+ + QK+TY
Sbjct: 669 NYPSFSILLGNTTQLYTRTVANVGPANSTYTAEIGVPVGVGMSLSPAQLTFTEVGQKLTY 728

Query: 723 SVTFKRIGSVSPPIEFAQGYLKWVSAKHVVRSPISVKFV 755
           SV+F           FAQG LKWVS K+ VRSPIS  F+
Sbjct: 729 SVSFIPFSEDRDNHTFAQGSLKWVSGKYSVRSPISFIFL 750

BLAST of Spg003072 vs. ExPASy Swiss-Prot
Match: G7KEU7 (Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1)

HSP 1 Score: 819.7 bits (2116), Expect = 2.7e-236
Identity = 437/730 (59.86%), Postives = 527/730 (72.19%), Query Frame = 0

Query: 33  NLQTYIVHVKKPETRANRQTDDDLENWHKSFLPTSSESSEERPELLYSYRNVMSGFAARL 92
           NL TYIVHVKK E  A+ Q+ +DL +W+ SFLP   ++   +  +++SYR V SGFA +L
Sbjct: 34  NLMTYIVHVKKSENVASHQS-EDLHSWYHSFLP---QTFPHKERMVFSYRKVASGFAVKL 93

Query: 93  SEEQVKAMEEKDGFVSARRERILHLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGG 152
           + E+ K+++EK   VSAR ER L LHTTHTP FLGL +  G W D N GKGVIIG++D G
Sbjct: 94  TPEEAKSLQEKGEIVSARPERTLELHTTHTPTFLGLKQGQGLWSDDNLGKGVIIGIIDTG 153

Query: 153 ITPSHPSFDDAGMPPPPAKWKGRCEF-NFSACNNKLIGARSFNLAAKALKGETTMDDSPI 212
           I P HPSF+D GMPPPPAKWKG CEF     CNNKLIGAR  NL   A++      + P 
Sbjct: 154 IFPLHPSFNDEGMPPPPAKWKGHCEFTGGQVCNNKLIGAR--NLVKSAIQ------EPPF 213

Query: 213 DEDGHGTHTASTAAGTFVDGADALANAKGTAVGMAPLAHLAIYKVCFGE-DCSDSDILAA 272
           +   HGTHTA+ AAG F++ A    NAKG A GMAP AHLAIYKVC  +  C++S ILAA
Sbjct: 214 ENFFHGTHTAAEAAGRFIEDASVFGNAKGVAAGMAPNAHLAIYKVCNDKIGCTESAILAA 273

Query: 273 LDAAVEDGVDVLSLSLGGESLPFFADDIAVGAFAAIQKGIFVSCSAANAGPFNATLSNEA 332
           +D A+EDGVDVLSLSLG  SLPFF D IA+GAFAA Q G+FVSCSAAN+GP  +TLSNEA
Sbjct: 274 MDIAIEDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQNGVFVSCSAANSGPGYSTLSNEA 333

Query: 333 PWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQPSDFPPTFLPLVYA-----GEKNQTA 392
           PWILTV ASTIDR+IVA+AKLGNGEE++GE+LFQP DF    LPLVY      G + Q  
Sbjct: 334 PWILTVGASTIDRKIVASAKLGNGEEYEGETLFQPKDFSQQLLPLVYPGSFGYGNQTQNQ 393

Query: 393 ALCGEGSLKDIDVKGKAVVCERGGGIARIAKGVEVKNAGGAAMILLNQEQDGFSTQADAH 452
           +LC  GSLK+ID+ GK V+C+  G ++ I KG EV N+GG AMIL N E  GFST A AH
Sbjct: 394 SLCLPGSLKNIDLSGKVVLCD-VGNVSSIVKGQEVLNSGGIAMILANSEALGFSTFAIAH 453

Query: 453 VLPATHVSHAAALKIKAYINSTTNPTATILFKGTVIGDDFSPAIASFSSRGPSLASPGIL 512
           VLPA  VS+AA L IK+YI ST NPTAT++FKGT+IGD  +P++  FSSRGPS  SPGIL
Sbjct: 454 VLPAVEVSYAAGLTIKSYIKSTYNPTATLIFKGTIIGDSLAPSVVYFSSRGPSQESPGIL 513

Query: 513 KPDITGPGVSILAAWPFPLDNNTNTKSTFNIISGTSMSCPHLSGIAALIKSSHPDWSPAA 572
           KPDI GPGV+ILAAW   +DN       F+I+SGTSMSCPHLSGIAALIKSSHPDWSPAA
Sbjct: 514 KPDIIGPGVNILAAWAVSVDNKI---PAFDIVSGTSMSCPHLSGIAALIKSSHPDWSPAA 573

Query: 573 IKSAIMTTANITNIEGQPIPDQNLQPADLFAIGAGHVNPSKAADPGLVYDIQPDDYIPYL 632
           IKSAIMTTAN  N+ G PI DQ L PAD+FA GAGHVNP KA DPGLVYDI+P+DY+PYL
Sbjct: 574 IKSAIMTTANTLNLGGIPILDQRLFPADIFATGAGHVNPVKANDPGLVYDIEPEDYVPYL 633

Query: 633 CGLGYKNNEVGIIAHKPIDCLAKPSIPEGELNYPSFTVALG-PPQTFTRTVTNVGGGYEV 692
           CGLGY + E+ +I    + C    SIPE +LNYPSF++ LG   Q +TRT+TNVG     
Sbjct: 634 CGLGYSDKEIEVIVQWKVKCSNVKSIPEAQLNYPSFSILLGSDSQYYTRTLTNVGFANST 693

Query: 693 YAAVVEAPPSVSVTVRPRKIFFSMMNQKVTYSVTF-KRIGSVSPPIEFAQGYLKWVSAKH 752
           Y   +E P ++ ++V P +I F+ +N+KV++SV F  +I        F QG L WVS +H
Sbjct: 694 YKVELEVPLALGMSVNPSEITFTEVNEKVSFSVEFIPQIKENRRNHTFGQGSLTWVSDRH 747

Query: 753 VVRSPISVKF 754
            VR PISV F
Sbjct: 754 AVRIPISVIF 747

BLAST of Spg003072 vs. ExPASy TrEMBL
Match: A0A6J1H242 (subtilisin-like protease SBT1.8 OS=Cucurbita moschata OX=3662 GN=LOC111459745 PE=3 SV=1)

HSP 1 Score: 1292.3 bits (3343), Expect = 0.0e+00
Identity = 658/754 (87.27%), Postives = 685/754 (90.85%), Query Frame = 0

Query: 1   MIVLPSLFLLLLLDIRGGGAQVTELPLRPVLSNLQTYIVHVKKPETRANRQTDDDLENWH 60
           M+  PSLFLLLLL+    GA VTELP    L NLQTYIVHVKKPE      T DDLE WH
Sbjct: 1   MVAFPSLFLLLLLNFHYVGALVTELP----LINLQTYIVHVKKPE------TTDDLEIWH 60

Query: 61  KSFLPTSSESSEERPELLYSYRNVMSGFAARLSEEQVKAMEEKDGFVSARRERILHLHTT 120
           +SFLP+SS        LLYSYRNVMSGFAARLSEEQVKAMEE DGFVSARRERIL LHTT
Sbjct: 61  RSFLPSSS-------SLLYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTT 120

Query: 121 HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPSHPSFDDAGMPPPPAKWKGRCEFNF 180
           HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGI PSHPSFDD GMPPPP KWKGRCEFNF
Sbjct: 121 HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNF 180

Query: 181 SACNNKLIGARSFNLAAKALKGETTMDDSPIDEDGHGTHTASTAAGTFVDGADALANAKG 240
           SACNNKLIGARSFNLA KALKGET MDDSPIDEDGHGTHTASTAAG FV GA+AL NAKG
Sbjct: 181 SACNNKLIGARSFNLATKALKGETMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKG 240

Query: 241 TAVGMAPLAHLAIYKVCFGEDCSDSDILAALDAAVEDGVDVLSLSLGGESLPFFADDIAV 300
           TAVGMAPLAHLAIYKVCFGEDC D+DILAALDAA+EDGVDVLSLSLG  S+PFF D +A+
Sbjct: 241 TAVGMAPLAHLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAI 300

Query: 301 GAFAAIQKGIFVSCSAANAGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGE 360
           GAFAAIQKGIFVSCSAAN+GPF ATLSNEAPWILTVAASTIDRRI AAAKLGNGEEFDGE
Sbjct: 301 GAFAAIQKGIFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGE 360

Query: 361 SLFQPSDFPPTFLPLVYAGEKNQTAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGVEV 420
           SLFQPSDFPPT LPLVYAGEKNQTAALCGEGSLKDIDVKGK VVCERGGGIARIAKG EV
Sbjct: 361 SLFQPSDFPPTLLPLVYAGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEV 420

Query: 421 KNAGGAAMILLNQEQDGFSTQADAHVLPATHVSHAAALKIKAYINSTTNPTATILFKGTV 480
           KNAGGAAMILLNQ+QDGFST+ADAHVLPA+HVSH AALKIKAYINSTT PTATILFKGTV
Sbjct: 421 KNAGGAAMILLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTV 480

Query: 481 IGDD-FSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISG 540
           IGDD FSPAIASFSSRGPS+ASPGILKPDITGPGVSILAAWPFPLD N NTKSTFNIISG
Sbjct: 481 IGDDNFSPAIASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKNRNTKSTFNIISG 540

Query: 541 TSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTANITNIEGQPIPDQNLQPADLFAIGA 600
           TSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTA+ITN+EGQPI D+NLQPADLFA GA
Sbjct: 541 TSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNLEGQPIVDENLQPADLFATGA 600

Query: 601 GHVNPSKAADPGLVYDIQPDDYIPYLCGLGYKNNEVGIIAHKPIDCLAKPSIPEGELNYP 660
           GHVNPSKAADPGLVYDIQPDDYIPYLCGLGYK+NEV  IA KPI+CLAKPSIPEG+LNYP
Sbjct: 601 GHVNPSKAADPGLVYDIQPDDYIPYLCGLGYKSNEVATIARKPINCLAKPSIPEGDLNYP 660

Query: 661 SFTVALGPPQTFTRTVTNVGGGYEVYAAVVEAPPSVSVTVRPRKIFFSMMNQKVTYSVTF 720
           SFTV LGPPQTFTRTVTNVG G EVY AVVEAPPS+SVT+RP KIFFS +NQKVT+SVTF
Sbjct: 661 SFTVVLGPPQTFTRTVTNVGCGREVYTAVVEAPPSISVTIRPSKIFFSKINQKVTFSVTF 720

Query: 721 KRIGSVSPPIEFAQGYLKWVSAKHVVRSPISVKF 754
           KRIGS+SP  EF +GYLKWVS KHVVRSPIS KF
Sbjct: 721 KRIGSISPSTEFGKGYLKWVSDKHVVRSPISFKF 737

BLAST of Spg003072 vs. ExPASy TrEMBL
Match: A0A5A7TJ55 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003250 PE=3 SV=1)

HSP 1 Score: 1274.6 bits (3297), Expect = 0.0e+00
Identity = 642/755 (85.03%), Postives = 684/755 (90.60%), Query Frame = 0

Query: 1   MIVLPSLFLLLLLDIRGGGAQVTELPLRPVLSNLQTYIVHVKKPETRANRQTDDDLENWH 60
           M++LPSLFLLLLL+  G  AQVTELP    LSNL TYIVHVKKPE        DDLE WH
Sbjct: 1   MVLLPSLFLLLLLNFHGYEAQVTELP----LSNLHTYIVHVKKPE------VVDDLEIWH 60

Query: 61  KSFLPTSSESSEERPELLYSYRNVMSGFAARLSEEQVKAMEEKDGFVSARRERILHLHTT 120
           +SFLPTS ++ EE+P LLYSYRNVMSGF+ARL+EE VKAMEEKDGFVSARRE I+HLHTT
Sbjct: 61  RSFLPTSLDNEEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTT 120

Query: 121 HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPSHPSFDDAGMPPPPAKWKGRCEFNF 180
           HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITP+HPSFDDAGMP PPAKWKGRCEFNF
Sbjct: 121 HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEFNF 180

Query: 181 SACNNKLIGARSFNLAAKALKGE-TTMDDSPIDEDGHGTHTASTAAGTFVDGADALANAK 240
           SACNNKLIGARS NLA++ALKG+ TT+DDSPIDEDGHGTHTASTAAGTFVDGA+AL NA 
Sbjct: 181 SACNNKLIGARSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAF 240

Query: 241 GTAVGMAPLAHLAIYKVCFGEDCSDSDILAALDAAVEDGVDVLSLSLGGESLPFFADDIA 300
           GTAVGMAPLAHLAIYKVCFGEDCSD DILA LDAAVEDGVDVLS+SLGG S+PFFAD  A
Sbjct: 241 GTAVGMAPLAHLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITA 300

Query: 301 VGAFAAIQKGIFVSCSAANAGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDG 360
           +G+FAAIQKGIFVSCSAAN+GPFNATLSNEAPWILTVAASTIDR+I A AKLGNGEEFDG
Sbjct: 301 IGSFAAIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDG 360

Query: 361 ESLFQPSDFPPTFLPLVYAGEKNQTAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGVE 420
           ESLFQP+DFP T LPLV+ GEKN+T ALC EGSLK+IDVKGK VVCERGGGIARIAKGVE
Sbjct: 361 ESLFQPNDFPQTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVE 420

Query: 421 VKNAGGAAMILLNQEQDGFSTQADAHVLPATHVSHAAALKIKAYINSTTNPTATILFKGT 480
           VKN GGAAMILLN E DGF+T+ DAHVLPA+HVSH AALKIKAYINSTT PTATILFKGT
Sbjct: 421 VKNGGGAAMILLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGT 480

Query: 481 VIGDDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISG 540
            IGDDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISG
Sbjct: 481 TIGDDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISG 540

Query: 541 TSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTANITNIEGQPIPDQNLQPADLFAIGA 600
           TSMSCPHLSGIAALIKS+HPDWSPAAIKS+IMTTANITN+EG PI D+ LQPADLFAIGA
Sbjct: 541 TSMSCPHLSGIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGA 600

Query: 601 GHVNPSKAADPGLVYDIQPDDYIPYLCGLGYKNNEVGIIAHKPIDCLAKPSIPEGELNYP 660
           GHVNPSKA DPGLVYDIQPDDYIPYLCGLGY NN+V +IAHKPIDCL   SIPEGELNYP
Sbjct: 601 GHVNPSKAIDPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYP 660

Query: 661 SFTVALGPPQTFTRTVTNVGGGYEVYAAVVEAPPSVSVTVRPRKIFFSMMNQKVTYSVTF 720
           SF V LGP QTF+RTVT+VG G  VY  V+EAP  VSVTVRPRK+ FS +NQK TYSVTF
Sbjct: 661 SFMVKLGPVQTFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTF 720

Query: 721 KRIGSVSPPIEFAQGYLKWVSAKHVVRSPISVKFV 755
           KRIGS+SP IEFA+GYLKWVSAKHVVRSPISVKFV
Sbjct: 721 KRIGSISPSIEFAEGYLKWVSAKHVVRSPISVKFV 745

BLAST of Spg003072 vs. ExPASy TrEMBL
Match: A0A1S3ATZ7 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482686 PE=3 SV=1)

HSP 1 Score: 1269.2 bits (3283), Expect = 0.0e+00
Identity = 640/755 (84.77%), Postives = 682/755 (90.33%), Query Frame = 0

Query: 1   MIVLPSLFLLLLLDIRGGGAQVTELPLRPVLSNLQTYIVHVKKPETRANRQTDDDLENWH 60
           M++LPSLFLLLLL+  G  AQVTELP    LSNL TYIVHVKKPE        DDLE WH
Sbjct: 1   MVLLPSLFLLLLLNFHGYEAQVTELP----LSNLHTYIVHVKKPE------VVDDLEIWH 60

Query: 61  KSFLPTSSESSEERPELLYSYRNVMSGFAARLSEEQVKAMEEKDGFVSARRERILHLHTT 120
           +SFLPTS ++ EE+P LLYSYRNVMSGF+ARL+EE VKAMEEKDGFVSARRE I+HLHTT
Sbjct: 61  RSFLPTSLDNEEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTT 120

Query: 121 HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPSHPSFDDAGMPPPPAKWKGRCEFNF 180
           HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITP+HPSFDDAGM  PPAKWKGRCEFNF
Sbjct: 121 HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMAQPPAKWKGRCEFNF 180

Query: 181 SACNNKLIGARSFNLAAKALKGE-TTMDDSPIDEDGHGTHTASTAAGTFVDGADALANAK 240
           SACNNKLIGARS NLA++ALKG+ TT+DDSPIDEDGHGTHTASTAAGTFVDGA+AL NA 
Sbjct: 181 SACNNKLIGARSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAF 240

Query: 241 GTAVGMAPLAHLAIYKVCFGEDCSDSDILAALDAAVEDGVDVLSLSLGGESLPFFADDIA 300
           GTAVGMAPLAHLAIYKVCFGEDCSD DILA LDAAVEDGVDVLS+SLGG S+PFFAD  A
Sbjct: 241 GTAVGMAPLAHLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITA 300

Query: 301 VGAFAAIQKGIFVSCSAANAGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDG 360
           +G+FAAIQKGIFVSCSAAN+GPFNATLSNEAPWILTVAASTIDR+I A AKLGNGEEFDG
Sbjct: 301 IGSFAAIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDG 360

Query: 361 ESLFQPSDFPPTFLPLVYAGEKNQTAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGVE 420
           ESLFQP+DFP T LPLV+ GEKN+T ALC EGSLK+IDVKGK VVCERGGGIARIAKGVE
Sbjct: 361 ESLFQPNDFPQTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVE 420

Query: 421 VKNAGGAAMILLNQEQDGFSTQADAHVLPATHVSHAAALKIKAYINSTTNPTATILFKGT 480
           VKN GGAAMILLN E DGF+T+ DAHVLPA+HVSH AALKIKAYINSTT PTATILFKGT
Sbjct: 421 VKNGGGAAMILLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGT 480

Query: 481 VIGDDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISG 540
            IGDDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISG
Sbjct: 481 TIGDDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISG 540

Query: 541 TSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTANITNIEGQPIPDQNLQPADLFAIGA 600
           TSMSCPHLSGIAALIKS+HPDWSPAAIKS+IMTTANITN+EG PI D+ LQPADLFAIGA
Sbjct: 541 TSMSCPHLSGIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPILDETLQPADLFAIGA 600

Query: 601 GHVNPSKAADPGLVYDIQPDDYIPYLCGLGYKNNEVGIIAHKPIDCLAKPSIPEGELNYP 660
           GHVNPSKA DPGLVYDIQPDDYIPYLCGLGY NN+V +IAHKPIDCL   SIPEGELNYP
Sbjct: 601 GHVNPSKAIDPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTSSIPEGELNYP 660

Query: 661 SFTVALGPPQTFTRTVTNVGGGYEVYAAVVEAPPSVSVTVRPRKIFFSMMNQKVTYSVTF 720
           SF V LGP QTF+RTVT+VG G  VY  V+EAP  VSVTVRPRK+ FS +NQK TYSVTF
Sbjct: 661 SFMVKLGPVQTFSRTVTSVGSGRVVYNVVIEAPEGVSVTVRPRKLSFSALNQKATYSVTF 720

Query: 721 KRIGSVSPPIEFAQGYLKWVSAKHVVRSPISVKFV 755
           KR GS+SP IEFA+GYLKWVSAKHVVRSPISVKFV
Sbjct: 721 KRSGSISPSIEFAEGYLKWVSAKHVVRSPISVKFV 745

BLAST of Spg003072 vs. ExPASy TrEMBL
Match: A0A6J1E0E3 (subtilisin-like protease SBT1.7 OS=Momordica charantia OX=3673 GN=LOC111025997 PE=3 SV=1)

HSP 1 Score: 1264.2 bits (3270), Expect = 0.0e+00
Identity = 643/760 (84.61%), Postives = 687/760 (90.39%), Query Frame = 0

Query: 1   MIVLPSLFLLLLLDIRGGGAQVTELPLRPVLSNLQTYIVHVKKPETRANRQTDDDLENWH 60
           M+VLPSLFLL+L    GGGA       RP  SNLQTYIVHVKKPE  +     +DLENWH
Sbjct: 1   MVVLPSLFLLVLFHFHGGGA-------RPTSSNLQTYIVHVKKPEKLSTYGQVEDLENWH 60

Query: 61  KSFLPTSSESSEERPELLYSYRNVMSGFAARLSEEQVKAMEEKDGFVSARRERILHLHTT 120
           +SFLP+   SSE+ P+LLYS+ NVMSGFAARL+EE VKAMEEKDGF+SARRERIL LHTT
Sbjct: 61  RSFLPS---SSEDHPKLLYSFXNVMSGFAARLTEEHVKAMEEKDGFISARRERILKLHTT 120

Query: 121 HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPSHPSFDDAGMPPPPAKWKGRCEFNF 180
           HTP+FLGLNRQFGFWKDSNFGKG IIGVLDGGITP+HPSFDDAGMPPPP KWKGRCEFNF
Sbjct: 121 HTPNFLGLNRQFGFWKDSNFGKGXIIGVLDGGITPNHPSFDDAGMPPPPVKWKGRCEFNF 180

Query: 181 SACNNKLIGARSFNLAAKALKGE-TTMDDSPIDEDGHGTHTASTAAGTFVDGADALANAK 240
           SACNNKLIGARSFNLA KALKGE  T  DSPIDEDGHGTHTASTAAG FVD A+AL NAK
Sbjct: 181 SACNNKLIGARSFNLATKALKGEQPTATDSPIDEDGHGTHTASTAAGAFVDEAEALGNAK 240

Query: 241 GTAVGMAPLAHLAIYKVCFGEDCSDSDILAALDAAVEDGVDVLSLSLGGESLPFFADDIA 300
           GTAVGMAPLAHLAIYKVCFGEDC DSDILAALDAAVEDGVDVLSLSLG +S PFF DDIA
Sbjct: 241 GTAVGMAPLAHLAIYKVCFGEDCPDSDILAALDAAVEDGVDVLSLSLGSDSAPFFRDDIA 300

Query: 301 VGAFAAIQKGIFVSCSAANAGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDG 360
           +GAFAA QKGIFVSCSA+N+GP NATLSNEAPWILTVAASTIDRRI+A AKLGNGEEFDG
Sbjct: 301 IGAFAATQKGIFVSCSASNSGPLNATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDG 360

Query: 361 ESLFQPSDFPPT-FLPLVYAGEKNQTAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGV 420
           ESLFQPSDFPPT FLPLVYAGEKN+TAALCGEGSLKDIDVKGKAVVCERGGGIARIAKG+
Sbjct: 361 ESLFQPSDFPPTLFLPLVYAGEKNETAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGM 420

Query: 421 EVKNAGGAAMILLNQEQDGFSTQADAHVLPATHVSHAAALKIKAYINSTTNPTATILFKG 480
           EVKNAGGAAMILLNQ+ DGF+T+ADAHVLPATHV+H AALKIK YINST NP A I F+G
Sbjct: 421 EVKNAGGAAMILLNQQTDGFTTEADAHVLPATHVNHVAALKIKDYINSTHNPIAAIAFRG 480

Query: 481 TVIG-DDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNT-NTKSTFNI 540
           TVIG DDFSPAIASFSSRGPSLASPGILKPDITGPG+SILAAWPFPLDNNT NTK TFN+
Sbjct: 481 TVIGDDDFSPAIASFSSRGPSLASPGILKPDITGPGISILAAWPFPLDNNTKNTKPTFNV 540

Query: 541 ISGTSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTANITNIEGQPIPDQNLQPADLFA 600
           ISGTSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTA+ITN +G+PI DQN QPADLFA
Sbjct: 541 ISGTSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTADITNHKGRPILDQNFQPADLFA 600

Query: 601 IGAGHVNPSKAADPGLVYDIQPDDYIPYLCGLGYKNNEVGIIAHKPIDCLAKPSIPEGEL 660
            GAGHVNPSKA DPGLVYDIQPDDYIPYLCGLGYK+NEV IIAH+P+DC AKPSIPEGEL
Sbjct: 601 TGAGHVNPSKATDPGLVYDIQPDDYIPYLCGLGYKDNEVSIIAHQPVDCSAKPSIPEGEL 660

Query: 661 NYPSFTVALGPPQTFTRTVTNVGGGYEVYAAVVEAPPS-VSVTVRPRKIFFSMMNQKVTY 720
           NYPSF+VALGPPQTFTRTVTNVGGGYEVY  ++EAPPS VSVTVRPRKIFFS +NQKV Y
Sbjct: 661 NYPSFSVALGPPQTFTRTVTNVGGGYEVYTVMIEAPPSGVSVTVRPRKIFFSAVNQKVAY 720

Query: 721 SVTFKRIGSVSPPIE-FAQGYLKWVSAKHVVRSPISVKFV 755
           SVTFKRIGS++PP    AQ YLK +S+KH+VRSPIS+KFV
Sbjct: 721 SVTFKRIGSITPPSSGSAQAYLKCMSSKHIVRSPISIKFV 750

BLAST of Spg003072 vs. ExPASy TrEMBL
Match: A0A0A0KKE3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157240 PE=3 SV=1)

HSP 1 Score: 1263.1 bits (3267), Expect = 0.0e+00
Identity = 636/755 (84.24%), Postives = 682/755 (90.33%), Query Frame = 0

Query: 1   MIVLPSLFLLLLLDIRGGGAQVTELPLRPVLSNLQTYIVHVKKPETRANRQTDDDLENWH 60
           M++LPSLFLLLLL+     AQVTELP     SNL TYIVHVKKPE        DDLE+WH
Sbjct: 1   MVLLPSLFLLLLLNFHVYEAQVTELP----FSNLHTYIVHVKKPE------VVDDLESWH 60

Query: 61  KSFLPTSSESSEERPELLYSYRNVMSGFAARLSEEQVKAMEEKDGFVSARRERILHLHTT 120
           +SFLPTS E+SEE+P LLYSYRNVMSGF+ARL+EE VKAMEEKDGFVSARRE I+HLHTT
Sbjct: 61  RSFLPTSLENSEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTT 120

Query: 121 HTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPSHPSFDDAGMPPPPAKWKGRCEFNF 180
           H+P+FLGLNRQFGFWKDSNFGKGVIIGVLDGGITPSHPSF DAGMP PPAKWKGRCEFNF
Sbjct: 121 HSPNFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNF 180

Query: 181 SACNNKLIGARSFNLAAKALKGE-TTMDDSPIDEDGHGTHTASTAAGTFVDGADALANAK 240
           SACNNKLIGARS NLA++ALKG+ TT+DDSPIDEDGHGTHTASTAAGTFVDGA+AL NA 
Sbjct: 181 SACNNKLIGARSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAF 240

Query: 241 GTAVGMAPLAHLAIYKVCFGEDCSDSDILAALDAAVEDGVDVLSLSLGGESLPFFADDIA 300
           GTAVGMAPLAHLAIYKVCFGE CS+ DILA LDAAVEDGVDVLS+SLGG  +PFFAD  A
Sbjct: 241 GTAVGMAPLAHLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITA 300

Query: 301 VGAFAAIQKGIFVSCSAANAGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDG 360
           +GAFAAIQKGIFVSCSAAN+GPFNATLSNEAPWILTVAASTIDR+I A AKLGNGEEFDG
Sbjct: 301 IGAFAAIQKGIFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDG 360

Query: 361 ESLFQPSDFPPTFLPLVYAGEKNQTAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGVE 420
           ESLFQP+DFP TFLPLV+ GEKN+T ALC EGSLK+IDVKGK VVC+RGGGIARIAKGVE
Sbjct: 361 ESLFQPNDFPQTFLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVE 420

Query: 421 VKNAGGAAMILLNQEQDGFSTQADAHVLPATHVSHAAALKIKAYINSTTNPTATILFKGT 480
           VKNAGGAAMILLN E DGF+T+ADAHVLPA+HVSH AALKIKAYINSTT PTATI+FKGT
Sbjct: 421 VKNAGGAAMILLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGT 480

Query: 481 VIGDDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIISG 540
            IGDDFSPAIA+FSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNI+SG
Sbjct: 481 TIGDDFSPAIAAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTKSTFNIVSG 540

Query: 541 TSMSCPHLSGIAALIKSSHPDWSPAAIKSAIMTTANITNIEGQPIPDQNLQPADLFAIGA 600
           TSMSCPHLSGIAALIKS+HPDWSPAAIKS+IMTTANITN+EG PI DQ LQPADLFAIGA
Sbjct: 541 TSMSCPHLSGIAALIKSAHPDWSPAAIKSSIMTTANITNLEGNPIVDQTLQPADLFAIGA 600

Query: 601 GHVNPSKAADPGLVYDIQPDDYIPYLCGLGYKNNEVGIIAHKPIDCLAKPSIPEGELNYP 660
           GHVNPSKA DPGLVYDIQPDDYIPYLCGLGY NN+V +IAHKPIDCL   SIPEGELNYP
Sbjct: 601 GHVNPSKAVDPGLVYDIQPDDYIPYLCGLGYTNNQVSLIAHKPIDCLTTTSIPEGELNYP 660

Query: 661 SFTVALGPPQTFTRTVTNVGGGYEVYAAVVEAPPSVSVTVRPRKIFFSMMNQKVTYSVTF 720
           SF V LG  QTF+RTVT VG G EVY  V+EAP  VSVTVRPRK+ FS +NQK TYSVTF
Sbjct: 661 SFMVKLGQVQTFSRTVTYVGSGREVYNVVIEAPEGVSVTVRPRKVIFSALNQKATYSVTF 720

Query: 721 KRIGSVSPPIEFAQGYLKWVSAKHVVRSPISVKFV 755
           KRIGS+SP  EFA+GYLKWVSAKH+VRSPISVKFV
Sbjct: 721 KRIGSISPSTEFAEGYLKWVSAKHLVRSPISVKFV 745

BLAST of Spg003072 vs. TAIR 10
Match: AT1G04110.1 (Subtilase family protein )

HSP 1 Score: 589.7 bits (1519), Expect = 3.3e-168
Identity = 341/754 (45.23%), Postives = 460/754 (61.01%), Query Frame = 0

Query: 35  QTYIVHVKKPETRANRQTDDDLENWHKSFLPTS---SESSEERP--ELLYSYRNVMSGFA 94
           QTYIV +      A  +T     +WH SFL  +    E  EE P   LLYSY + + GFA
Sbjct: 26  QTYIVQLHPNSETA--KTFASKFDWHLSFLQEAVLGVEEEEEEPSSRLLYSYGSAIEGFA 85

Query: 95  ARLSEEQVKAMEEKDGFVSARRERILHLHTTHTPDFLGLN--RQFGFWKDSNFGKGVIIG 154
           A+L+E + + +      V+ R + +L + TT++  FLGL+     G W  S FG+G IIG
Sbjct: 86  AQLTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFGNSGVWSKSRFGQGTIIG 145

Query: 155 VLDGGITPSHPSFDDAGMPPPPAKWKGRCE----FNFSACNNKLIGARSFNLAAKALKGE 214
           VLD G+ P  PSFDD GMP  P KWKG C+    F+ S+CN KLIGAR F    +     
Sbjct: 146 VLDTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNRKLIGARFFIRGHRVANSP 205

Query: 215 TTMDDSP------IDEDGHGTHTASTAAGTFVDGADALANAKGTAVGMAPLAHLAIYKVC 274
               + P       D  GHGTHTAST  G+ V  A+ L N  G A GMAP AH+A+YKVC
Sbjct: 206 EESPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAGVARGMAPGAHIAVYKVC 265

Query: 275 FGEDCSDSDILAALDAAVEDGVDVLSLSLGGESLPFFADDIAVGAFAAIQKGIFVSCSAA 334
           +   C  SDILAA+D A++D VDVLSLSLGG  +P + D IA+G F A+++GI V C+A 
Sbjct: 266 WFNGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAIGTFRAMERGISVICAAG 325

Query: 335 NAGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQPSDFPPT--FLPL 394
           N GP  ++++N APW+ T+ A T+DRR  A  +L NG+   GESL+           + +
Sbjct: 326 NNGPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGKLLYGESLYPGKGIKNAGREVEV 385

Query: 395 VYAGEKNQTAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGVEVKNAGGAAMILLNQEQ 454
           +Y    ++ +  C  GSL   +++GK V+C+RG    R  KG  VK AGG AMIL N E 
Sbjct: 386 IYVTGGDKGSEFCLRGSLPREEIRGKMVICDRGVN-GRSEKGEAVKEAGGVAMILANTEI 445

Query: 455 DGFSTQADAHVLPATHVSHAAALKIKAYINSTTNPTATILFKGTVIGDDFSPAIASFSSR 514
           +      D H+LPAT + +  ++ +KAY+N+T  P A I+F GTVIG   +P +A FS+R
Sbjct: 446 NQEEDSIDVHLLPATLIGYTESVLLKAYVNATVKPKARIIFGGTVIGRSRAPEVAQFSAR 505

Query: 515 GPSLASPGILKPDITGPGVSILAAWP-------FPLDNNTNTKSTFNIISGTSMSCPHLS 574
           GPSLA+P ILKPD+  PGV+I+AAWP        P D+    +  F ++SGTSMSCPH+S
Sbjct: 506 GPSLANPSILKPDMIAPGVNIIAAWPQNLGPTGLPYDSR---RVNFTVMSGTSMSCPHVS 565

Query: 575 GIAALIKSSHPDWSPAAIKSAIMTTANITNIEGQPIPDQNLQPADLFAIGAGHVNPSKAA 634
           GI ALI+S++P+WSPAAIKSA+MTTA++ + +G+ I D N +PA +FAIGAGHVNP KA 
Sbjct: 566 GITALIRSAYPNWSPAAIKSALMTTADLYDRQGKAIKDGN-KPAGVFAIGAGHVNPQKAI 625

Query: 635 DPGLVYDIQPDDYIPYLCGLGYKNNEVGIIAHKPIDC---LAKPSIPEGELNYPSFTVAL 694
           +PGLVY+IQP DYI YLC LG+  +++  I HK + C   L K   P   LNYPS  V  
Sbjct: 626 NPGLVYNIQPVDYITYLCTLGFTRSDILAITHKNVSCNGILRKN--PGFSLNYPSIAVIF 685

Query: 695 GPPQT---FTRTVTNVGGGYEVYAAVVEAPPSVSVTVRPRKIFFSMMNQKVTYSVTF--K 752
              +T    TR VTNVG    +Y+  V+AP  + V V P+++ F  ++Q ++Y V F  K
Sbjct: 686 KRGKTTEMITRRVTNVGSPNSIYSVNVKAPEGIKVIVNPKRLVFKHVDQTLSYRVWFVLK 745

BLAST of Spg003072 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 582.0 bits (1499), Expect = 6.8e-166
Identity = 335/740 (45.27%), Postives = 456/740 (61.62%), Query Frame = 0

Query: 32  SNLQTYIVHVKKPETRANRQTDDDLENWHKSFLPTSSESSEERPELLYSYRNVMSGFAAR 91
           S+  TYIVH+ K +  +   + D   NW+ S L + S+S+    ELLY+Y N + GF+ R
Sbjct: 27  SDQGTYIVHMAKSQMPS---SFDLHSNWYDSSLRSISDSA----ELLYTYENAIHGFSTR 86

Query: 92  LSEEQVKAMEEKDGFVSARRERILHLHTTHTPDFLGLNRQFG-FWKDSNFGKGVIIGVLD 151
           L++E+  ++  + G +S   E    LHTT TP FLGL+      + ++     V++GVLD
Sbjct: 87  LTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVVVGVLD 146

Query: 152 GGITPSHPSFDDAGMPPPPAKWKGRCE----FNFSACNNKLIGARSFNLAAKALKG---E 211
            G+ P   S+ D G  P P+ WKG CE    F  S CN KLIGAR F    ++  G   E
Sbjct: 147 TGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDE 206

Query: 212 TTMDDSPIDEDGHGTHTASTAAGTFVDGADALANAKGTAVGMAPLAHLAIYKVCFGEDCS 271
           +    SP D+DGHGTHT+STAAG+ V+GA  L  A GTA GMAP A +A+YKVC+   C 
Sbjct: 207 SKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCF 266

Query: 272 DSDILAALDAAVEDGVDVLSLSLGGESLPFFADDIAVGAFAAIQKGIFVSCSAANAGPFN 331
            SDILAA+D A+ D V+VLS+SLGG    ++ D +A+GAFAA+++GI VSCSA NAGP +
Sbjct: 267 SSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSS 326

Query: 332 ATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQPSDFPPTFLPLVYAGEKNQ 391
           ++LSN APWI TV A T+DR   A A LGNG+ F G SLF+    P   LP +YAG  + 
Sbjct: 327 SSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNASN 386

Query: 392 --TAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGVEVKNAGGAAMILLNQEQDGFSTQ 451
                LC  G+L    VKGK V+C+RG   AR+ KG  VK AGG  MIL N   +G    
Sbjct: 387 ATNGNLCMTGTLIPEKVKGKIVMCDRGIN-ARVQKGDVVKAAGGVGMILANTAANGEELV 446

Query: 452 ADAHVLPATHVSHAAALKIKAYINSTTNPTATILFKGTVIGDDFSPAIASFSSRGPSLAS 511
           ADAH+LPAT V   A   I+ Y+ +  NPTA+I   GTV+G   SP +A+FSSRGP+  +
Sbjct: 447 ADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSIT 506

Query: 512 PGILKPDITGPGVSILAAW-----PFPLDNNTNTKSTFNIISGTSMSCPHLSGIAALIKS 571
           P ILKPD+  PGV+ILAAW     P  L +++  +  FNIISGTSMSCPH+SG+AAL+KS
Sbjct: 507 PNILKPDLIAPGVNILAAWTGAAGPTGLASDSR-RVEFNIISGTSMSCPHVSGLAALLKS 566

Query: 572 SHPDWSPAAIKSAIMTTANITNIEGQPIPD-QNLQPADLFAIGAGHVNPSKAADPGLVYD 631
            HP+WSPAAI+SA+MTTA  T  +G+P+ D    +P+  F  GAGHV+P+ A +PGL+YD
Sbjct: 567 VHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYD 626

Query: 632 IQPDDYIPYLCGLGYKNNEVGIIAHKPIDCLAKPSIPEGELNYPSFTVALG--PPQTFTR 691
           +  +DY+ +LC L Y + ++  ++ +   C    S    +LNYPSF V +       +TR
Sbjct: 627 LTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTR 686

Query: 692 TVTNVGGGYEVYAAVVEAPPSVSVTVRPRKIFFSMMNQKVTYSVTFKRIGSVSPPIEFAQ 751
           TVT+VGG       V      V ++V P  + F   N+K +Y+VTF  + S  P    + 
Sbjct: 687 TVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTF-TVDSSKPSGSNSF 746

Query: 752 GYLKWVSAKHVVRSPISVKF 754
           G ++W   KHVV SP+++ +
Sbjct: 747 GSIEWSDGKHVVGSPVAISW 756

BLAST of Spg003072 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 580.1 bits (1494), Expect = 2.6e-165
Identity = 345/751 (45.94%), Postives = 459/751 (61.12%), Query Frame = 0

Query: 34  LQTYIVHVKKPETRANRQTDDDLENWHKSFLPTSSESSEERPELLYSYRNVMSGFAARLS 93
           L++YIVHV++    +   + +   NWH S L  S  SS +   LLYSY   + GF+ARLS
Sbjct: 30  LESYIVHVQRSHKPSLFSSHN---NWHVSLL-RSLPSSPQPATLLYSYSRAVHGFSARLS 89

Query: 94  EEQVKAMEEKDGFVSARRERILHLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGI 153
             Q  A+      +S   ++   +HTTHTP FLG ++  G W +SN+G+ VI+GVLD GI
Sbjct: 90  PIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYGEDVIVGVLDTGI 149

Query: 154 TPSHPSFDDAGMPPPPAKWKGRCE----FNFSACNNKLIGARSFNLAAKALKGETTM--- 213
            P HPSF D+G+ P P+ WKG CE    F  S+CN KLIGAR+F       +  T     
Sbjct: 150 WPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYLTQRNGTKKHAA 209

Query: 214 --DDSPIDEDGHGTHTASTAAGTFVDGADALANAKGTAVGMAPLAHLAIYKVCFGEDCSD 273
               SP D +GHGTHTASTAAG+ V  A     A+GTA GMA  A +A YK+C+   C D
Sbjct: 210 KESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAAYKICWTGGCYD 269

Query: 274 SDILAALDAAVEDGVDVLSLSLG--GESLPFFADDIAVGAFAAIQKGIFVSCSAANAGPF 333
           SDILAA+D AV DGV V+SLS+G  G +  +  D IA+GAF A + GI VSCSA N+GP 
Sbjct: 270 SDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIVVSCSAGNSGPN 329

Query: 334 NATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQPSDFPPTFLPLVYAGEKN 393
             T +N APWILTV AST+DR   A A  G+G+ F G SL+     P + L LVY+G+  
Sbjct: 330 PETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDSQLSLVYSGDCG 389

Query: 394 QTAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGVEVKNAGGAAMILLNQEQDGFSTQA 453
             + LC  G L    V+GK V+C+RGG  AR+ KG  VK AGGA MIL N  + G    A
Sbjct: 390 --SRLCYPGKLNSSLVEGKIVLCDRGGN-ARVEKGSAVKLAGGAGMILANTAESGEELTA 449

Query: 454 DAHVLPATHVSHAAALKIKAYINSTTNPTATILFKGTVIG-DDFSPAIASFSSRGPSLAS 513
           D+H++PAT V   A  +I+ YI ++ +PTA I F GT+IG    SP +A+FSSRGP+  +
Sbjct: 450 DSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSPRVAAFSSRGPNHLT 509

Query: 514 PGILKPDITGPGVSILAAW-----PFPLDNNTNTKSTFNIISGTSMSCPHLSGIAALIKS 573
           P ILKPD+  PGV+ILA W     P  LD +   +  FNIISGTSMSCPH+SG+AAL++ 
Sbjct: 510 PVILKPDVIAPGVNILAGWTGMVGPTDLDIDPR-RVQFNIISGTSMSCPHVSGLAALLRK 569

Query: 574 SHPDWSPAAIKSAIMTTANITNIEGQPIPD-QNLQPADLFAIGAGHVNPSKAADPGLVYD 633
           +HPDWSPAAIKSA++TTA      G+PI D    + ++ F  GAGHV+P+KA +PGLVYD
Sbjct: 570 AHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHVDPNKALNPGLVYD 629

Query: 634 IQPDDYIPYLCGLGYKNNEVGIIAHKPI---DCLAKPSIPEGELNYPSFTV---ALGPPQ 693
           I+  +Y+ +LC +GY+   + +    P     C        G+LNYPSF+V   + G   
Sbjct: 630 IEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPSFSVVFASTGEVV 689

Query: 694 TFTRTVTNVGGGYE-VYAAVVEAPPSVSVTVRPRKIFFSMMNQKVTYSVTFKR------I 753
            + R V NVG   + VY   V++P +V + V P K+ FS     + Y VTFK       +
Sbjct: 690 KYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGV 749

BLAST of Spg003072 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 575.5 bits (1482), Expect = 6.4e-164
Identity = 333/734 (45.37%), Postives = 449/734 (61.17%), Query Frame = 0

Query: 35  QTYIVHVKKPETRANRQTDDDLENWHKSFLPTSSESSEERPELLYSYRNVMSGFAARL-S 94
           +TYI+ V   +   +  T  D   W+ S L  +SESS     LLY+Y     GF+A L S
Sbjct: 28  KTYIIRVNHSDKPESFLTHHD---WYTSQL--NSESS-----LLYTYTTSFHGFSAYLDS 87

Query: 95  EEQVKAMEEKDGFVSARRERILHLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGI 154
            E    +   +  +    + +  LHTT TP+FLGLN +FG     +   GVIIGVLD G+
Sbjct: 88  TEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGV 147

Query: 155 TPSHPSFDDAGMPPPPAKWKGRCE----FNFSACNNKLIGARSFNLAAKALKG----ETT 214
            P   SFDD  MP  P+KWKG CE    F+   CN KLIGARSF+   +   G       
Sbjct: 148 WPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKR 207

Query: 215 MDDSPIDEDGHGTHTASTAAGTFVDGADALANAKGTAVGMAPLAHLAIYKVCFGEDCSDS 274
              SP D DGHGTHT++TAAG+ V  A  L  A GTA GMA  A +A YKVC+   C  S
Sbjct: 208 ESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGS 267

Query: 275 DILAALDAAVEDGVDVLSLSLGGESLPFFADDIAVGAFAAIQKGIFVSCSAANAGPFNAT 334
           DILAA+D A+ DGVDVLSLSLGG S P++ D IA+GAF+A+++G+FVSCSA N+GP  A+
Sbjct: 268 DILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRAS 327

Query: 335 LSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLFQPSDFPPTFLPLVYAGEKNQTA 394
           ++N APW++TV A T+DR   A A LGNG+   G SL+         L LVY    + ++
Sbjct: 328 VANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSS 387

Query: 395 ALCGEGSLKDIDVKGKAVVCERGGGIARIAKGVEVKNAGGAAMILLNQEQDGFSTQADAH 454
            LC  GSL    V+GK VVC+RG   AR+ KG  V++AGG  MI+ N    G    AD+H
Sbjct: 388 NLCLPGSLDSSIVRGKIVVCDRGVN-ARVEKGAVVRDAGGLGMIMANTAASGEELVADSH 447

Query: 455 VLPATHVSHAAALKIKAYINSTTNPTATILFKGTVIGDDFSPAIASFSSRGPSLASPGIL 514
           +LPA  V       ++ Y+ S + PTA ++FKGTV+    SP +A+FSSRGP+  +P IL
Sbjct: 448 LLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEIL 507

Query: 515 KPDITGPGVSILAAW-----PFPLDNNTNTKSTFNIISGTSMSCPHLSGIAALIKSSHPD 574
           KPD+ GPGV+ILA W     P  LD ++  ++ FNI+SGTSMSCPH+SG+A L+K++HP+
Sbjct: 508 KPDVIGPGVNILAGWSDAIGPTGLDKDSR-RTQFNIMSGTSMSCPHISGLAGLLKAAHPE 567

Query: 575 WSPAAIKSAIMTTANITNIEGQPIPD-QNLQPADLFAIGAGHVNPSKAADPGLVYDIQPD 634
           WSP+AIKSA+MTTA + +    P+ D  +   ++ +A G+GHV+P KA  PGLVYDI  +
Sbjct: 568 WSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTE 627

Query: 635 DYIPYLCGLGYKNNEVGIIAHKP-IDCLAKPSIPEGELNYPSFTVALGPPQT--FTRTVT 694
           +YI +LC L Y  + +  I  +P ++C  K S P G+LNYPSF+V  G  +   +TR VT
Sbjct: 628 EYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDP-GQLNYPSFSVLFGGKRVVRYTREVT 687

Query: 695 NVGGGYEVYAAVVEAPPSVSVTVRPRKIFFSMMNQKVTYSVTFKRIGSVSPPIEFAQGYL 751
           NVG    VY   V   PSV ++V+P K+ F  + +K  Y+VTF     VS   +   G +
Sbjct: 688 NVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSI 747

BLAST of Spg003072 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 568.9 bits (1465), Expect = 6.0e-162
Identity = 338/754 (44.83%), Postives = 451/754 (59.81%), Query Frame = 0

Query: 32  SNLQTYIVHVKKPETRANRQTDDDLENWHKSFLPTSSESSEERPELLYSYRNVMSGFAAR 91
           SN  TYIVHV   +  A         +W+ S L + + S    P ++++Y  V  GF+AR
Sbjct: 23  SNSLTYIVHV---DHEAKPSIFPTHFHWYTSSLASLTSSP---PSIIHTYDTVFHGFSAR 82

Query: 92  LSEEQVKAMEEKDGFVSARRERILHLHTTHTPDFLGLNR--QFGFWKDSNFGKGVIIGVL 151
           L+ +    + +    +S   E++ HLHTT +P+FLGL    + G  ++S+FG  ++IGV+
Sbjct: 83  LTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVI 142

Query: 152 DGGITPSHPSFDDAGMPPPPAKWKGRC----EFNFSACNNKLIGARSFNLAAKALKG--- 211
           D G+ P  PSFDD G+ P P KWKG+C    +F  SACN KL+GAR F    +A  G   
Sbjct: 143 DTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMN 202

Query: 212 ETTMDDSPIDEDGHGTHTASTAAGTFVDGADALANAKGTAVGMAPLAHLAIYKVCFGEDC 271
           ETT   SP D DGHGTHTAS +AG +V  A  L  A G A GMAP A LA YKVC+   C
Sbjct: 203 ETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGC 262

Query: 272 SDSDILAALDAAVEDGVDVLSLSLGGESLPFFADDIAVGAFAAIQKGIFVSCSAANAGPF 331
            DSDILAA D AV DGVDV+SLS+GG  +P++ D IA+GAF AI +GIFVS SA N GP 
Sbjct: 263 YDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPG 322

Query: 332 NATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEFDGESLF-QPSDFPPTFLPLVYAGE- 391
             T++N APW+ TV A TIDR   A  KLGNG+   G S++  P   P    PLVY G  
Sbjct: 323 ALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSL 382

Query: 392 ---KNQTAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGVEVKNAGGAAMILLNQEQDG 451
                 +++LC EGSL    VKGK V+C+RG   +R  KG  V+  GG  MI+ N   DG
Sbjct: 383 LGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGIN-SRATKGEIVRKNGGLGMIIANGVFDG 442

Query: 452 FSTQADAHVLPATHVSHAAALKIKAYIN------STTNPTATILFKGTVIGDDFSPAIAS 511
               AD HVLPAT V  +   +I+ YI+      S+ +PTATI+FKGT +G   +P +AS
Sbjct: 443 EGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVAS 502

Query: 512 FSSRGPSLASPGILKPDITGPGVSILAAWPFPLD----NNTNTKSTFNIISGTSMSCPHL 571
           FS+RGP+  +P ILKPD+  PG++ILAAWP  +      + N ++ FNI+SGTSM+CPH+
Sbjct: 503 FSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHV 562

Query: 572 SGIAALIKSSHPDWSPAAIKSAIMTTANITNIEGQPIPDQNL-QPADLFAIGAGHVNPSK 631
           SG+AAL+K++HPDWSPAAI+SA++TTA   +  G+P+ D++    + +   G+GHV+P+K
Sbjct: 563 SGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTK 622

Query: 632 AADPGLVYDIQPDDYIPYLCGLGYKNNEVGIIAHKPIDC-LAKPSIPEGELNYPSFTVAL 691
           A DPGLVYDI   DYI +LC   Y    +  I  +  DC  A+ +   G LNYPSF+V  
Sbjct: 623 AMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVF 682

Query: 692 GP------PQTFTRTVTNVGGGYEVYAAVVEAPPSVSVTVRPRKIFFSMMNQKVTYSVTF 751
                      F RTVTNVG    VY   +  P   +VTV P K+ F  + QK+++ V  
Sbjct: 683 QQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRV 742