Spg002875 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg002875
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationscaffold6: 617257 .. 620367 (+)
RNA-Seq ExpressionSpg002875
SyntenySpg002875
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGCACCAAATCAACGGTGCCATTATTCGCTCTCTCTCTCTCTCTCTCTCTCTCTCACGCTCTTCAGAGGCCTGGGAAGCGAGCAACAACACAACACAGGGGGTAGAGAGACGAAGAAAAGAACCTCATCTTTCCGGTAACAAAGGGTTTTTTTTTATTCCTGGGAATCCATCAATCTACCCTTTTTTTGTGCTCTTTTCATTCTTTGCTTCTTTCCCACTCTCTGAGAAATTTGCAGTAACTGGTGAAACTATTGAAGAGGGGAATCCTGAAATTTTCTGAGATCTTGATCGATTCTGTCTTTGGATAAGTGGGTTTGTGTTCAGTTCCTTAGGTTTTCTGTTTCGTTAATGGATTTAGACAGTATCGAGTGTGTGTCATCGTCAGATGGTCTCGATGAGGATGAGATCCATCTGCACCACACTCTTCATCCTTATTCCCATTCTCATCATCACCCAGAGTTGTCAGCGTCGAAGCCTCGCAATGGAAACAACAATAGCGCTGTGAACGGCCCCACTGCAACTGCGCCGGCAACTAGCGTCCATGAATTGCTGGAGTGCCCTGTCTGCACTAATTCTATGTATCCTCCCATCCATCAGGTTTGTCTTCGTTGCCCTAATTATTTCAGAATGGAGATTATTTGGCCCTCCACCCTCCATATTTGGATTTTTGACTTGGGTATGAAAAGGGGTTTTTTAAAATTTTTCCTGCTCGGGTTTTGGATTATCGTTGATCGATCAATTGTTGATCGGAAGTTGTGTGGCATTGTATACTGACAACCCCTATGATATGTAAGTGAATGGATATTACTGAGACATGAAGGCACCCCAATGGACATTACTTTTACAAATAAAAGATAATCATGAATGCATTTTGATGTTGCTGTGCTTTGGTCTTGAAAATTGTATGAGCTAGAAACTAAGGCCTCGTTTGATAACCATTTGGTTTTTGGTTTTTGAAAATTAAGCTTATAAATCCTACTTACACCTACAAATTTCTTTGTTTTTCTAATATCCATCTTTTCATGTTTTAAAAAACCAAGCCATTTTTTGAAAACTAAAAAAAGTAGTTTTCAAAAACTTGTTTTTGTTTTTGGAATTTGGCTAGAAATTCAAATGTGTCTTTGACAAAGATGAACACCATAGTAGGGAAATTTGGAAAAAACAAGCATAATTTTCAAAAACCAAATGGTTATCAAACGGGGCCTAAGTTATCTTAGTTTTTGGCTTGTTGTATGTTGTATCAAGTAGTCAATAAACCACCATCAAATTAGATTAGTACTTGTGCCGTTTGTGCTTTTAAAGCCTTTATTTCTAGCATAAGATGCTTGTAGTTTCTACAAATTTGCTAAGGAATTTCTTTTTGGTTTAGTTATAAACATTATTTTGGTAGGCTGATGAAATTTTACTTTTCGCCTTGCTTCCTTTGGGATATCATTTTCCATCATCATAGTTTTCTCTCTTCTTCTTGTATCATTTGAACTAACCTTTATAGGATGTACGTGTCTGTATAATCAGGATGAAAGCAGTTGTGAATATGCTTGTTTTATACCAATAATTGTCTGAGGACATATGTTGTTTCTTATAAGACGTGCATTTTATTGGAGATATATTTTCAATGTTAGTGTATAAATTTTGGTTAACTGTATGTCCAACTAAAATGTTGCAGTGCCACAATGGACATACCTTATGTTCTACATGTAAAACACGGGTTCATAATCGCTGCCCTACTTGTAGACAAGAGCTTGGAGATATTAGGTGCTTAGCACTGGAGAAGGTGGCAGAGTCACTTGAATTGCCTTGCAAGTATTATTCCTTGGGGTGCCCTGAGATATTTCCATACTACAGTAAACTTAAGCATGAGGCTTTGTGCAACTTCAGACCCTATAGTTGCCCCTACGCTGGGTCGGAATGCTCTGCTGTTGGGGATATTCCTTTCCTTGTTGCCCATCTGAGGGACGATCATAAGGTGGACATGCACACTGGCTGCACGTTTAACCATCGCTATGTGAAATCCAATCCAAGGGAAGTGGAGAATGCCACTTGGATGCTTACTGTAAGTCTGATATATCTGACCTCAACTATCAATTGGATTCTCATTTTCTCTTTCTTTGGTAGAACTTAGTTTATCTACGTGAATCGATCCTTACCTGTGATAGATTTGTATGCACACGTCTGTATGTTTATGTAAATATGTATGTATGTATCTGTGGTGTCGCTCGATATCTATTAAGGTATGGGGAGGACAAGGGTTTCAAACCAAACTATGAAATTCTGGGAGATTAGAAGAAGTCAACAACTAGTTGAAAACAAAAGAAAGATGATGAAAAGTCCGTTATTACATAACGTGCTGCAAGTTTTATAGCCCATCAAAGCTGCAAGTTGATTAGAAATTTGGTAATGGATCATTTTATTTTATTTTATGGAACTTTGACCTTCACTGTGTTTGATGATTGAATTATCAAAGGATTGTACATGTTTTTATATAATTTTTTTCAGTTTCCTTTTACCCCAAGTGATGGATTTATGCTTTTGCGGTTCAAGTGTTTCTCTCAAATCTGTACAAACGAAAGCTAATAAGGTCACGGGATGCCAAAACTACCCATAAATGCCATATATGGCTTGTTATGTGTTTGATCAAATAAGGCCTTCGCGCCAGAAGGAGACTCATTCTGCTGGACATTCCTCAGGGTTAAATCCATATTTTAGATTGATCCCGTTTCTTGTGGCTTGCTCTTCTCACATTTTGCAGGTTTTTCATTGTTTCGGTCAATACTTCTGCCTTCACTTTGAAGCCTTTCAGCTTGGCATGGCCCCAGTCTACATGGCTTTCCTTCGATTCATGGGTGATGAGAATGAGGCTCGAACCTACAGCTACAGTCTCGAGGTTGGTGGATATGGGCGGAAACTGATATGGGAGGGTACACCACGAAGTATTAGAGATAGTCATCGAAAAGTCAGGGACAGCCACGATGGATTGATCATTCAGAGAAATATGGCTCTTTTCTTCTCTGGTGGGGATAGAAAGGAACTGAAGCTGAGAGTTACAGGAAGGATATGGAAGGAACAAAACCCAGATGCTGGGGTCTGCATACCGAACCTTTGTAGCTAA

mRNA sequence

ATGGATTTAGACAGTATCGAGTGTGTGTCATCGTCAGATGGTCTCGATGAGGATGAGATCCATCTGCACCACACTCTTCATCCTTATTCCCATTCTCATCATCACCCAGAGTTGTCAGCGTCGAAGCCTCGCAATGGAAACAACAATAGCGCTGTGAACGGCCCCACTGCAACTGCGCCGGCAACTAGCGTCCATGAATTGCTGGAGTGCCCTGTCTGCACTAATTCTATGTATCCTCCCATCCATCAGTGCCACAATGGACATACCTTATGTTCTACATGTAAAACACGGGTTCATAATCGCTGCCCTACTTGTAGACAAGAGCTTGGAGATATTAGGTGCTTAGCACTGGAGAAGGTGGCAGAGTCACTTGAATTGCCTTGCAAGTATTATTCCTTGGGGTGCCCTGAGATATTTCCATACTACAGTAAACTTAAGCATGAGGCTTTGTGCAACTTCAGACCCTATAGTTGCCCCTACGCTGGGTCGGAATGCTCTGCTGTTGGGGATATTCCTTTCCTTGTTGCCCATCTGAGGGACGATCATAAGGTGGACATGCACACTGGCTGCACGTTTAACCATCGCTATGTGAAATCCAATCCAAGGGAAGTGGAGAATGCCACTTGGATGCTTACTGTTTTTCATTGTTTCGGTCAATACTTCTGCCTTCACTTTGAAGCCTTTCAGCTTGGCATGGCCCCAGTCTACATGGCTTTCCTTCGATTCATGGGTGATGAGAATGAGGCTCGAACCTACAGCTACAGTCTCGAGGTTGGTGGATATGGGCGGAAACTGATATGGGAGGGTACACCACGAAGTATTAGAGATAGTCATCGAAAAGTCAGGGACAGCCACGATGGATTGATCATTCAGAGAAATATGGCTCTTTTCTTCTCTGGTGGGGATAGAAAGGAACTGAAGCTGAGAGTTACAGGAAGGATATGGAAGGAACAAAACCCAGATGCTGGGGTCTGCATACCGAACCTTTGTAGCTAA

Coding sequence (CDS)

ATGGATTTAGACAGTATCGAGTGTGTGTCATCGTCAGATGGTCTCGATGAGGATGAGATCCATCTGCACCACACTCTTCATCCTTATTCCCATTCTCATCATCACCCAGAGTTGTCAGCGTCGAAGCCTCGCAATGGAAACAACAATAGCGCTGTGAACGGCCCCACTGCAACTGCGCCGGCAACTAGCGTCCATGAATTGCTGGAGTGCCCTGTCTGCACTAATTCTATGTATCCTCCCATCCATCAGTGCCACAATGGACATACCTTATGTTCTACATGTAAAACACGGGTTCATAATCGCTGCCCTACTTGTAGACAAGAGCTTGGAGATATTAGGTGCTTAGCACTGGAGAAGGTGGCAGAGTCACTTGAATTGCCTTGCAAGTATTATTCCTTGGGGTGCCCTGAGATATTTCCATACTACAGTAAACTTAAGCATGAGGCTTTGTGCAACTTCAGACCCTATAGTTGCCCCTACGCTGGGTCGGAATGCTCTGCTGTTGGGGATATTCCTTTCCTTGTTGCCCATCTGAGGGACGATCATAAGGTGGACATGCACACTGGCTGCACGTTTAACCATCGCTATGTGAAATCCAATCCAAGGGAAGTGGAGAATGCCACTTGGATGCTTACTGTTTTTCATTGTTTCGGTCAATACTTCTGCCTTCACTTTGAAGCCTTTCAGCTTGGCATGGCCCCAGTCTACATGGCTTTCCTTCGATTCATGGGTGATGAGAATGAGGCTCGAACCTACAGCTACAGTCTCGAGGTTGGTGGATATGGGCGGAAACTGATATGGGAGGGTACACCACGAAGTATTAGAGATAGTCATCGAAAAGTCAGGGACAGCCACGATGGATTGATCATTCAGAGAAATATGGCTCTTTTCTTCTCTGGTGGGGATAGAAAGGAACTGAAGCTGAGAGTTACAGGAAGGATATGGAAGGAACAAAACCCAGATGCTGGGGTCTGCATACCGAACCTTTGTAGCTAA

Protein sequence

MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAPATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKVAESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Homology
BLAST of Spg002875 vs. NCBI nr
Match: XP_022141336.1 (E3 ubiquitin-protein ligase SINAT5-like [Momordica charantia])

HSP 1 Score: 707.2 bits (1824), Expect = 6.6e-200
Identity = 327/331 (98.79%), Postives = 330/331 (99.70%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNG+NNSA+ GPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGSNNSAITGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPY+CPYAGSECSAVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of Spg002875 vs. NCBI nr
Match: XP_023541988.1 (E3 ubiquitin-protein ligase SINAT5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 702.2 bits (1811), Expect = 2.1e-198
Identity = 325/331 (98.19%), Postives = 328/331 (99.09%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAP 60
           MDLDSIECVSSSDGLDED+IHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVN PTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDDIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNDPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           +TSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  STSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNP+EVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPKEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQN DAG CIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNADAGACIPNLCS 331

BLAST of Spg002875 vs. NCBI nr
Match: XP_022984623.1 (E3 ubiquitin-protein ligase SINAT5 isoform X1 [Cucurbita maxima])

HSP 1 Score: 702.2 bits (1811), Expect = 2.1e-198
Identity = 324/331 (97.89%), Postives = 330/331 (99.70%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELS++KPRNG+NNSA+ GPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSSTKPRNGSNNSAITGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPY+CPYAGSECSAVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVF+CFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of Spg002875 vs. NCBI nr
Match: XP_022945143.1 (E3 ubiquitin-protein ligase SINAT5-like [Cucurbita moschata] >XP_023541986.1 E3 ubiquitin-protein ligase SINAT5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 701.0 bits (1808), Expect = 4.7e-198
Identity = 324/331 (97.89%), Postives = 329/331 (99.40%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLH YSHSHHHPELSA+KPRNGNNNSAV GPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHSYSHSHHHPELSATKPRNGNNNSAVAGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPC+YYSLGCPEIFPYYSKLKHEALCNFRPY+CPYAGSECSAVGDIPFLV+HLRD
Sbjct: 121 AESLELPCRYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVSHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGL+IQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLMIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of Spg002875 vs. NCBI nr
Match: XP_022922771.1 (E3 ubiquitin-protein ligase SINAT5 isoform X1 [Cucurbita moschata] >XP_023553000.1 E3 ubiquitin-protein ligase SINAT5 isoform X1 [Cucurbita pepo subsp. pepo] >KAG7014703.1 E3 ubiquitin-protein ligase SINAT4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 700.7 bits (1807), Expect = 6.2e-198
Identity = 323/331 (97.58%), Postives = 330/331 (99.70%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELS++KPRNG+NNSA+ GPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSSTKPRNGSNNSAITGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPY+CPYAGSECSAVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMH+GCTFNHRYVKSNPREVENATWMLTVF+CFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of Spg002875 vs. ExPASy Swiss-Prot
Match: Q84JL3 (E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=1 SV=1)

HSP 1 Score: 571.6 bits (1472), Expect = 5.7e-162
Identity = 271/332 (81.63%), Postives = 286/332 (86.14%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAP 60
           MDLDS++C S+ D  D++EIH     +     HHH   + +     N N+A +G   T  
Sbjct: 1   MDLDSMDCTSTMDVTDDEEIHQDRHSYASVSKHHHTNNNTT-----NVNAAASGLLPT-- 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
            TSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCK RVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  TTSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPCK+ SLGCPEIFPYYSKLKHE +CNFRPYSCPYAGSECS  GDIPFLVAHLRD
Sbjct: 121 AESLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMH+GCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDE EAR Y+YSLEVGGYGRKLIWEGTPRS+RDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPD---AGVCIPNL 330
           GDRKELKLRVTGRIWKEQ       G CIPNL
Sbjct: 301 GDRKELKLRVTGRIWKEQQQSGEGGGACIPNL 325

BLAST of Spg002875 vs. ExPASy Swiss-Prot
Match: Q9STN8 (E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana OX=3702 GN=SINAT4 PE=1 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 2.6e-159
Identity = 270/336 (80.36%), Postives = 291/336 (86.61%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAV----NGPT 60
           M+ DS+ECVSS+           + +H   + H   + S++K   G   +AV     GPT
Sbjct: 1   METDSMECVSSTG----------NEIHQNGNGHQSYQFSSTKTHGGAAAAAVVTNIVGPT 60

Query: 61  ATAPATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLA 120
           ATAPATSV+ELLECPVCT SMYPPIHQCHNGHTLCSTCK RVHNRCPTCRQELGDIRCLA
Sbjct: 61  ATAPATSVYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIRCLA 120

Query: 121 LEKVAESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVA 180
           LEKVAESLELPCK+Y+LGCPEIFPYYSKLKHE+LCNFRPYSCPYAGSEC  VGDIPFLVA
Sbjct: 121 LEKVAESLELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPFLVA 180

Query: 181 HLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVY 240
           HLRDDHKVDMH G TFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGM PVY
Sbjct: 181 HLRDDHKVDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVY 240

Query: 241 MAFLRFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMAL 300
           MAFLRFMGDE +AR+YSYSLEVGG GRKL WEGTPRSIRDSHRKVRDS+DGLIIQRNMAL
Sbjct: 241 MAFLRFMGDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRNMAL 300

Query: 301 FFSGGDRKELKLRVTGRIWKEQ-NPDAGVCIPNLCS 332
           FFSGGDRKELKLRVTG+IWKEQ +PD+G+ IPNL S
Sbjct: 301 FFSGGDRKELKLRVTGKIWKEQHSPDSGLSIPNLSS 326

BLAST of Spg002875 vs. ExPASy Swiss-Prot
Match: Q8S3N1 (E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=1 SV=2)

HSP 1 Score: 552.4 bits (1422), Expect = 3.6e-156
Identity = 263/332 (79.22%), Postives = 285/332 (85.84%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAP 60
           M+ DSI+ V     +D+DEIH  H            + S++K + G            +P
Sbjct: 1   METDSIDSV-----IDDDEIHQKH------------QFSSTKSQGG-------ATVVISP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSV+ELLECPVCTNSMYPPIHQCHNGHTLCSTCK+RVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVYELLECPVCTNSMYPPIHQCHNGHTLCSTCKSRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPCKYY+LGC  IFPYYSKLKHE+ CNFRPYSCPYAGSEC+AVGDI FLVAHLRD
Sbjct: 121 AESLELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVF CFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDE++AR Y+YSLEVGG GRK  WEGTPRS+RDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKE-QNPDAGVCIPNLCS 332
           GD+KELKLRVTGRIWKE QNPD+GVCI ++CS
Sbjct: 301 GDKKELKLRVTGRIWKEQQNPDSGVCITSMCS 308

BLAST of Spg002875 vs. ExPASy Swiss-Prot
Match: Q10L91 (E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS1 PE=1 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 3.8e-134
Identity = 215/258 (83.33%), Postives = 234/258 (90.70%), Query Frame = 0

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ++SV ELLECPVC ++MYPPIHQC NGHTLCS CK RVHNRCPTCR ELG+IRCLALEKV
Sbjct: 44  SSSVRELLECPVCLSAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKV 103

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           A SLELPCKY + GC  I+PYY KLKHE+ C +RPYSCPYAGSEC+  GDIP+LV HL+D
Sbjct: 104 AASLELPCKYQNFGCVGIYPYYCKLKHESQCQYRPYSCPYAGSECTVAGDIPYLVNHLKD 163

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMH GCTFNHRYVKSNP EVENATWMLTVF CFGQYFCLHFEAFQLGMAPVY+AFL
Sbjct: 164 DHKVDMHNGCTFNHRYVKSNPHEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFL 223

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGD+ EA+ YSYSLEVGG GRK+IW+G PRSIRDSHRKVRDS+DGLIIQRNMALFFSG
Sbjct: 224 RFMGDDLEAKNYSYSLEVGGTGRKMIWQGVPRSIRDSHRKVRDSYDGLIIQRNMALFFSG 283

Query: 301 GDRKELKLRVTGRIWKEQ 319
           G+RKELKLRVTGRIWKEQ
Sbjct: 284 GERKELKLRVTGRIWKEQ 301

BLAST of Spg002875 vs. ExPASy Swiss-Prot
Match: Q9M2P4 (E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=1 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 4.0e-131
Identity = 219/287 (76.31%), Postives = 239/287 (83.28%), Query Frame = 0

Query: 37  ELSASKPRNGNN-----NSAVNGPTATAPATSVHELLECPVCTNSMYPPIHQCHNGHTLC 96
           E+  +K    NN      SA  G         V+ELLECPVCTN MYPPIHQC NGHTLC
Sbjct: 22  EVKTAKVEVNNNKPTKPGSAGIGKYGIHSNNGVYELLECPVCTNLMYPPIHQCPNGHTLC 81

Query: 97  STCKTRVHNRCPTCRQELGDIRCLALEKVAESLELPCKYYSLGCPEIFPYYSKLKHEALC 156
           S CK RV N CPTCR ELG+IRCLALEKVAESLE+PC+Y +LGC +IFPYYSKLKHE  C
Sbjct: 82  SNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCHDIFPYYSKLKHEQHC 141

Query: 157 NFRPYSCPYAGSECSAVGDIPFLVAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWML 216
            FRPY+CPYAGSECS  GDIP LV HL+DDHKVDMH GCTFNHRYVKSNP EVENATWML
Sbjct: 142 RFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWML 201

Query: 217 TVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDENEARTYSYSLEVGGYGRKLIWEGTP 276
           TVF+CFG+ FCLHFEAFQLGMAPVYMAFLRFMGDENEA+ +SYSLEVG +GRKL W+G P
Sbjct: 202 TVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHGRKLTWQGIP 261

Query: 277 RSIRDSHRKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 319
           RSIRDSHRKVRDS DGLII RN+AL+FSGGDR+ELKLRVTGRIWKE+
Sbjct: 262 RSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRVTGRIWKEE 308

BLAST of Spg002875 vs. ExPASy TrEMBL
Match: A0A6J1CIV8 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111011754 PE=3 SV=1)

HSP 1 Score: 707.2 bits (1824), Expect = 3.2e-200
Identity = 327/331 (98.79%), Postives = 330/331 (99.70%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNG+NNSA+ GPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGSNNSAITGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPY+CPYAGSECSAVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of Spg002875 vs. ExPASy TrEMBL
Match: A0A6J1JB27 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111482855 PE=3 SV=1)

HSP 1 Score: 702.2 bits (1811), Expect = 1.0e-198
Identity = 324/331 (97.89%), Postives = 330/331 (99.70%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELS++KPRNG+NNSA+ GPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSSTKPRNGSNNSAITGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPY+CPYAGSECSAVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVF+CFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of Spg002875 vs. ExPASy TrEMBL
Match: A0A6J1FZZ8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111449471 PE=3 SV=1)

HSP 1 Score: 701.0 bits (1808), Expect = 2.3e-198
Identity = 324/331 (97.89%), Postives = 329/331 (99.40%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLH YSHSHHHPELSA+KPRNGNNNSAV GPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHSYSHSHHHPELSATKPRNGNNNSAVAGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPC+YYSLGCPEIFPYYSKLKHEALCNFRPY+CPYAGSECSAVGDIPFLV+HLRD
Sbjct: 121 AESLELPCRYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVSHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGL+IQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLMIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of Spg002875 vs. ExPASy TrEMBL
Match: A0A6J1E513 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111430668 PE=3 SV=1)

HSP 1 Score: 700.7 bits (1807), Expect = 3.0e-198
Identity = 323/331 (97.58%), Postives = 330/331 (99.70%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAP 60
           MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELS++KPRNG+NNSA+ GPTATAP
Sbjct: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSSTKPRNGSNNSAITGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPY+CPYAGSECSAVGDIPFLVAHLRD
Sbjct: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMH+GCTFNHRYVKSNPREVENATWMLTVF+CFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFNCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of Spg002875 vs. ExPASy TrEMBL
Match: A0A6J1HVG5 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111467802 PE=3 SV=1)

HSP 1 Score: 699.5 bits (1804), Expect = 6.7e-198
Identity = 323/331 (97.58%), Postives = 328/331 (99.09%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAP 60
           MDLDSIECVSSSDG DEDEIHLHHTLH YSHSHHHPELSA+KPRNGNNNSAV GPTATAP
Sbjct: 1   MDLDSIECVSSSDGFDEDEIHLHHTLHSYSHSHHHPELSATKPRNGNNNSAVAGPTATAP 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPC+YYSLGCPEIFPYYSKLKHEALCNFRPY+CPYAGSECSAVGDIPFLV+HLRD
Sbjct: 121 AESLELPCRYYSLGCPEIFPYYSKLKHEALCNFRPYNCPYAGSECSAVGDIPFLVSHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGL+IQRNMALFFSG
Sbjct: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLMIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 332
           GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS
Sbjct: 301 GDRKELKLRVTGRIWKEQNPDAGVCIPNLCS 331

BLAST of Spg002875 vs. TAIR 10
Match: AT3G61790.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 571.6 bits (1472), Expect = 4.0e-163
Identity = 271/332 (81.63%), Postives = 286/332 (86.14%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAVNGPTATAP 60
           MDLDS++C S+ D  D++EIH     +     HHH   + +     N N+A +G   T  
Sbjct: 1   MDLDSMDCTSTMDVTDDEEIHQDRHSYASVSKHHHTNNNTT-----NVNAAASGLLPT-- 60

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
            TSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCK RVHNRCPTCRQELGDIRCLALEKV
Sbjct: 61  TTSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKV 120

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLELPCK+ SLGCPEIFPYYSKLKHE +CNFRPYSCPYAGSECS  GDIPFLVAHLRD
Sbjct: 121 AESLELPCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRD 180

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMH+GCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL
Sbjct: 181 DHKVDMHSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDE EAR Y+YSLEVGGYGRKLIWEGTPRS+RDSHRKVRDSHDGLIIQRNMALFFSG
Sbjct: 241 RFMGDETEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSG 300

Query: 301 GDRKELKLRVTGRIWKEQNPD---AGVCIPNL 330
           GDRKELKLRVTGRIWKEQ       G CIPNL
Sbjct: 301 GDRKELKLRVTGRIWKEQQQSGEGGGACIPNL 325

BLAST of Spg002875 vs. TAIR 10
Match: AT4G27880.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 562.8 bits (1449), Expect = 1.9e-160
Identity = 270/336 (80.36%), Postives = 291/336 (86.61%), Query Frame = 0

Query: 1   MDLDSIECVSSSDGLDEDEIHLHHTLHPYSHSHHHPELSASKPRNGNNNSAV----NGPT 60
           M+ DS+ECVSS+           + +H   + H   + S++K   G   +AV     GPT
Sbjct: 1   METDSMECVSSTG----------NEIHQNGNGHQSYQFSSTKTHGGAAAAAVVTNIVGPT 60

Query: 61  ATAPATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLA 120
           ATAPATSV+ELLECPVCT SMYPPIHQCHNGHTLCSTCK RVHNRCPTCRQELGDIRCLA
Sbjct: 61  ATAPATSVYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIRCLA 120

Query: 121 LEKVAESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVA 180
           LEKVAESLELPCK+Y+LGCPEIFPYYSKLKHE+LCNFRPYSCPYAGSEC  VGDIPFLVA
Sbjct: 121 LEKVAESLELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPFLVA 180

Query: 181 HLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVY 240
           HLRDDHKVDMH G TFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGM PVY
Sbjct: 181 HLRDDHKVDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVY 240

Query: 241 MAFLRFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMAL 300
           MAFLRFMGDE +AR+YSYSLEVGG GRKL WEGTPRSIRDSHRKVRDS+DGLIIQRNMAL
Sbjct: 241 MAFLRFMGDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRNMAL 300

Query: 301 FFSGGDRKELKLRVTGRIWKEQ-NPDAGVCIPNLCS 332
           FFSGGDRKELKLRVTG+IWKEQ +PD+G+ IPNL S
Sbjct: 301 FFSGGDRKELKLRVTGKIWKEQHSPDSGLSIPNLSS 326

BLAST of Spg002875 vs. TAIR 10
Match: AT3G58040.1 (seven in absentia of Arabidopsis 2 )

HSP 1 Score: 469.2 bits (1206), Expect = 2.8e-132
Identity = 219/287 (76.31%), Postives = 239/287 (83.28%), Query Frame = 0

Query: 37  ELSASKPRNGNN-----NSAVNGPTATAPATSVHELLECPVCTNSMYPPIHQCHNGHTLC 96
           E+  +K    NN      SA  G         V+ELLECPVCTN MYPPIHQC NGHTLC
Sbjct: 22  EVKTAKVEVNNNKPTKPGSAGIGKYGIHSNNGVYELLECPVCTNLMYPPIHQCPNGHTLC 81

Query: 97  STCKTRVHNRCPTCRQELGDIRCLALEKVAESLELPCKYYSLGCPEIFPYYSKLKHEALC 156
           S CK RV N CPTCR ELG+IRCLALEKVAESLE+PC+Y +LGC +IFPYYSKLKHE  C
Sbjct: 82  SNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCHDIFPYYSKLKHEQHC 141

Query: 157 NFRPYSCPYAGSECSAVGDIPFLVAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWML 216
            FRPY+CPYAGSECS  GDIP LV HL+DDHKVDMH GCTFNHRYVKSNP EVENATWML
Sbjct: 142 RFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWML 201

Query: 217 TVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDENEARTYSYSLEVGGYGRKLIWEGTP 276
           TVF+CFG+ FCLHFEAFQLGMAPVYMAFLRFMGDENEA+ +SYSLEVG +GRKL W+G P
Sbjct: 202 TVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHGRKLTWQGIP 261

Query: 277 RSIRDSHRKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 319
           RSIRDSHRKVRDS DGLII RN+AL+FSGGDR+ELKLRVTGRIWKE+
Sbjct: 262 RSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRVTGRIWKEE 308

BLAST of Spg002875 vs. TAIR 10
Match: AT2G41980.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 464.2 bits (1193), Expect = 9.1e-131
Identity = 209/258 (81.01%), Postives = 229/258 (88.76%), Query Frame = 0

Query: 61  ATSVHELLECPVCTNSMYPPIHQCHNGHTLCSTCKTRVHNRCPTCRQELGDIRCLALEKV 120
           +  V+ELLECPVCTN MYPPIHQC NGHTLCS+CK RV N CPTCR ELG+IRCLALEKV
Sbjct: 48  SNGVYELLECPVCTNLMYPPIHQCPNGHTLCSSCKLRVQNTCPTCRYELGNIRCLALEKV 107

Query: 121 AESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFLVAHLRD 180
           AESLE+PC+Y +LGC +IFPYYSKLKHE  C FR YSCPYAGSECS  GDIP LV HL+D
Sbjct: 108 AESLEVPCRYQNLGCQDIFPYYSKLKHEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLKD 167

Query: 181 DHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 240
           DHKVDMH GCTFNHRYVKSNP EVENATWMLTVF+CFG+ FCLHFEAFQLGMAPVYMAFL
Sbjct: 168 DHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFL 227

Query: 241 RFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNMALFFSG 300
           RFMGDENEA+ +SYSLEVG + RKL W+G PRSIRDSHRKVRDS DGLII RN+AL+FSG
Sbjct: 228 RFMGDENEAKKFSYSLEVGAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSG 287

Query: 301 GDRKELKLRVTGRIWKEQ 319
            D++ELKLRVTGRIWKE+
Sbjct: 288 SDKEELKLRVTGRIWKEE 305

BLAST of Spg002875 vs. TAIR 10
Match: AT5G53360.1 (TRAF-like superfamily protein )

HSP 1 Score: 425.6 bits (1093), Expect = 3.6e-119
Identity = 195/218 (89.45%), Postives = 207/218 (94.95%), Query Frame = 0

Query: 115 LALEKVAESLELPCKYYSLGCPEIFPYYSKLKHEALCNFRPYSCPYAGSECSAVGDIPFL 174
           +ALEKVAESLELPCKYY+LGC  IFPYYSKLKHE+ CNFRPYSCPYAGSEC+AVGDI FL
Sbjct: 15  IALEKVAESLELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFL 74

Query: 175 VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAP 234
           VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVF CFGQYFCLHFEAFQLGMAP
Sbjct: 75  VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAP 134

Query: 235 VYMAFLRFMGDENEARTYSYSLEVGGYGRKLIWEGTPRSIRDSHRKVRDSHDGLIIQRNM 294
           VYMAFLRFMGDE++AR Y+YSLEVGG GRK  WEGTPRS+RDSHRKVRDSHDGLIIQRNM
Sbjct: 135 VYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNM 194

Query: 295 ALFFSGGDRKELKLRVTGRIWKE-QNPDAGVCIPNLCS 332
           ALFFSGGD+KELKLRVTGRIWKE QNPD+GVCI ++CS
Sbjct: 195 ALFFSGGDKKELKLRVTGRIWKEQQNPDSGVCITSMCS 232

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141336.16.6e-20098.79E3 ubiquitin-protein ligase SINAT5-like [Momordica charantia][more]
XP_023541988.12.1e-19898.19E3 ubiquitin-protein ligase SINAT5-like [Cucurbita pepo subsp. pepo][more]
XP_022984623.12.1e-19897.89E3 ubiquitin-protein ligase SINAT5 isoform X1 [Cucurbita maxima][more]
XP_022945143.14.7e-19897.89E3 ubiquitin-protein ligase SINAT5-like [Cucurbita moschata] >XP_023541986.1 E3 ... [more]
XP_022922771.16.2e-19897.58E3 ubiquitin-protein ligase SINAT5 isoform X1 [Cucurbita moschata] >XP_023553000... [more]
Match NameE-valueIdentityDescription
Q84JL35.7e-16281.63E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=... [more]
Q9STN82.6e-15980.36E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana OX=3702 GN=SINAT4 PE=... [more]
Q8S3N13.6e-15679.22E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=... [more]
Q10L913.8e-13483.33E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS... [more]
Q9M2P44.0e-13176.31E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1CIV83.2e-20098.79RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101175... [more]
A0A6J1JB271.0e-19897.89RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111482855 P... [more]
A0A6J1FZZ82.3e-19897.89RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111449471... [more]
A0A6J1E5133.0e-19897.58RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111430668... [more]
A0A6J1HVG56.7e-19897.58RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111467802 P... [more]
Match NameE-valueIdentityDescription
AT3G61790.14.0e-16381.63Protein with RING/U-box and TRAF-like domains [more]
AT4G27880.11.9e-16080.36Protein with RING/U-box and TRAF-like domains [more]
AT3G58040.12.8e-13276.31seven in absentia of Arabidopsis 2 [more]
AT2G41980.19.1e-13181.01Protein with RING/U-box and TRAF-like domains [more]
AT5G53360.13.6e-11989.45TRAF-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008974TRAF-likeGENE3D2.60.210.10Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain Acoord: 177..311
e-value: 5.9E-38
score: 131.6
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 29..122
e-value: 3.4E-7
score: 31.8
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 123..176
e-value: 3.5E-17
score: 64.4
IPR018121Seven-in-absentia protein, TRAF-like domainPFAMPF03145Sinacoord: 112..311
e-value: 6.9E-79
score: 264.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 28..58
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 41..58
NoneNo IPR availablePANTHERPTHR10315E3 UBIQUITIN PROTEIN LIGASE SIAHcoord: 1..323
NoneNo IPR availablePANTHERPTHR10315:SF127E3 UBIQUITIN-PROTEIN LIGASEcoord: 1..323
NoneNo IPR availableCDDcd16571RING-HC_SIAHscoord: 68..106
e-value: 1.57992E-14
score: 64.6134
NoneNo IPR availableCDDcd03829Sinacoord: 184..312
e-value: 7.59574E-68
score: 206.854
NoneNo IPR availableSUPERFAMILY49599TRAF domain-likecoord: 123..315
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 59..110
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 70..106
score: 9.464913
IPR013010Zinc finger, SIAH-typePROSITEPS51081ZF_SIAHcoord: 123..183
score: 14.108719

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg002875.1Spg002875.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007275 multicellular organism development
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016874 ligase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding