Spg002855 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg002855 vs. NCBI nr
Match: XP_023542232.1 (uncharacterized protein LOC111802192 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 786.2 bits (2029), Expect = 1.6e-223
Identity = 414/476 (86.97%), Postives = 438/476 (92.02%), Query Frame = 0

Query: 1   MALETWLIKVKNAVSNKFDVVRT--STTVNVKP--TSSSSKKSPNVGVLAFEIAGLMSKL 60
           MALETWL KVKNAVSNKFDVVRT  STT+N +P  T+SSSKKSPNVGVLAFEIA LMSKL
Sbjct: 1   MALETWLSKVKNAVSNKFDVVRTSSSTTLNTQPTTTASSSKKSPNVGVLAFEIAALMSKL 60

Query: 61  LHLWQSLSDNNIIRLRNESISLEGVHKIVSNDEAFLLGLACAEMTENLRLVAKSVSRLSS 120
           LHLWQSLSD +IIRLRNES+SLEGVHKIVSNDE FLLGLACAEMTENLRL+A SVSRL  
Sbjct: 61  LHLWQSLSDTHIIRLRNESLSLEGVHKIVSNDEPFLLGLACAEMTENLRLLANSVSRLCI 120

Query: 121 KCDDADLRSFERLFDDFADSGRDLHNWILSEKEMECRNKRVERLVTVTASLHREMDELSI 180
           KC+DADLRSF R FDDFADSGRDLHNWIL+EKEMECRNKRVERLVTVTA+LHREMDE+S+
Sbjct: 121 KCNDADLRSFHRAFDDFADSGRDLHNWILNEKEMECRNKRVERLVTVTATLHREMDEVSV 180

Query: 181 MESGLRKAVANLQSCQQENNINPPPLESSLKEQKIVDLQQKILWQRQEIKYLKEKSLWNR 240
           ME+GLRKAVANLQSCQQEN        SS  EQKI+DLQQ+I+WQRQE+KYLKEKSLWNR
Sbjct: 181 MEAGLRKAVANLQSCQQEN--------SSPLEQKIIDLQQRIVWQRQEVKYLKEKSLWNR 240

Query: 241 TFDTVMSILARSIFTTLARIKLVFGLAQFPSSLPRSLSASAAVHPLKTNLNDNGKDYCPV 300
           TFDTVMSILARS+FTTLARIK VFGL QFPSSLPRSLSASAAVHPLK NLNDNGK++ PV
Sbjct: 241 TFDTVMSILARSVFTTLARIKFVFGLGQFPSSLPRSLSASAAVHPLK-NLNDNGKEF-PV 300

Query: 301 MSKNNTFFEPNSKLLRPPPTTLGASGLGLHYANLIIVMDKMIKSPQLVGVDARDDLYSML 360
               N FFEPNSKLL+PP +TLGA+GL LHYANLIIVMDKMIKSPQLVGVDARDDLYSML
Sbjct: 301 --SKNVFFEPNSKLLKPPMSTLGATGLALHYANLIIVMDKMIKSPQLVGVDARDDLYSML 360

Query: 361 PNSVRWALRARLRGVGFTASDPSLAGEWREAMGRILGWLSPLAQNMIKWQSERSFEQQNY 420
           PNSVR ALRARLRGVGFTASDPSLAGEWREAMGRIL WLSP+AQNMIKWQSERSFEQQNY
Sbjct: 361 PNSVRSALRARLRGVGFTASDPSLAGEWREAMGRILEWLSPMAQNMIKWQSERSFEQQNY 420

Query: 421 MAPKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTAKALFACNNFNST 473
           MAPKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTAKALFACNNF +T
Sbjct: 421 MAPKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTAKALFACNNFIAT 464

BLAST of Spg002855 vs. NCBI nr
Match: XP_022968635.1 (uncharacterized protein LOC111467797 [Cucurbita maxima])

HSP 1 Score: 785.0 bits (2026), Expect = 3.6e-223
Identity = 413/475 (86.95%), Postives = 437/475 (92.00%), Query Frame = 0

Query: 1   MALETWLIKVKNAVSNKFDVVRT--STTVNVKP-TSSSSKKSPNVGVLAFEIAGLMSKLL 60
           MALETWL KVKNAVSNKFDVVRT  STT+N +P T++SSKKSPNVGVLAFEIA LMSKLL
Sbjct: 1   MALETWLSKVKNAVSNKFDVVRTSSSTTLNTQPTTTASSKKSPNVGVLAFEIAALMSKLL 60

Query: 61  HLWQSLSDNNIIRLRNESISLEGVHKIVSNDEAFLLGLACAEMTENLRLVAKSVSRLSSK 120
           HLWQSLSD +IIRLRNES+SLEGVHKIVSNDE FLLGLACAEMTENLRL+A SVSRL  K
Sbjct: 61  HLWQSLSDTHIIRLRNESLSLEGVHKIVSNDEPFLLGLACAEMTENLRLLANSVSRLCIK 120

Query: 121 CDDADLRSFERLFDDFADSGRDLHNWILSEKEMECRNKRVERLVTVTASLHREMDELSIM 180
           C+DADLRSF R FDDFADSGRDLHNWIL EKEMECRNKRVERLVTVTA+LH+EMDELS+M
Sbjct: 121 CNDADLRSFHRAFDDFADSGRDLHNWILKEKEMECRNKRVERLVTVTATLHKEMDELSVM 180

Query: 181 ESGLRKAVANLQSCQQENNINPPPLESSLKEQKIVDLQQKILWQRQEIKYLKEKSLWNRT 240
           E+GLRKAVANLQSCQQEN        SS  EQKI+DLQQ+I+WQRQE+KYLKEKSLWNRT
Sbjct: 181 EAGLRKAVANLQSCQQEN--------SSPLEQKIIDLQQRIVWQRQEVKYLKEKSLWNRT 240

Query: 241 FDTVMSILARSIFTTLARIKLVFGLAQFPSSLPRSLSASAAVHPLKTNLNDNGKDYCPVM 300
           FDTVMSILARS+FTTLARIK VFGL QFPSSLPRSLSASAAVHPLK NLNDNGK++ PV 
Sbjct: 241 FDTVMSILARSVFTTLARIKFVFGLGQFPSSLPRSLSASAAVHPLK-NLNDNGKEF-PV- 300

Query: 301 SKNNTFFEPNSKLLRPPPTTLGASGLGLHYANLIIVMDKMIKSPQLVGVDARDDLYSMLP 360
              N FFEPNSKLL+PP +TLGA+GL LHYANLIIVMDKMIKSPQLVGVDARDDLYSMLP
Sbjct: 301 -SKNAFFEPNSKLLKPPMSTLGATGLALHYANLIIVMDKMIKSPQLVGVDARDDLYSMLP 360

Query: 361 NSVRWALRARLRGVGFTASDPSLAGEWREAMGRILGWLSPLAQNMIKWQSERSFEQQNYM 420
           NSVR ALRARLRGVGFTASDPSLAGEWREAMGRIL WLSP+AQNMIKWQSERSFEQQNYM
Sbjct: 361 NSVRSALRARLRGVGFTASDPSLAGEWREAMGRILEWLSPMAQNMIKWQSERSFEQQNYM 420

Query: 421 APKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTAKALFACNNFNST 473
           APKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTAKALFACNNF +T
Sbjct: 421 APKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTAKALFACNNFIAT 463

BLAST of Spg002855 vs. NCBI nr
Match: XP_022945953.1 (uncharacterized protein LOC111450042 isoform X1 [Cucurbita moschata])

HSP 1 Score: 784.3 bits (2024), Expect = 6.1e-223
Identity = 412/472 (87.29%), Postives = 436/472 (92.37%), Query Frame = 0

Query: 1   MALETWLIKVKNAVSNKFDVVRTS--TTVNVKP-TSSSSKKSPNVGVLAFEIAGLMSKLL 60
           MALETWL KVKNAVSNKFDVVRTS  TT+N +P T++S+KKSPNVGVLAFEIA LMSKLL
Sbjct: 1   MALETWLSKVKNAVSNKFDVVRTSSLTTLNTQPTTTASTKKSPNVGVLAFEIAALMSKLL 60

Query: 61  HLWQSLSDNNIIRLRNESISLEGVHKIVSNDEAFLLGLACAEMTENLRLVAKSVSRLSSK 120
           HLWQSLSD +IIRLRNES+SLEGVHKIVSNDE FLLGLACAEMTENLRL+A SVSRL  K
Sbjct: 61  HLWQSLSDTHIIRLRNESLSLEGVHKIVSNDEPFLLGLACAEMTENLRLLANSVSRLCIK 120

Query: 121 CDDADLRSFERLFDDFADSGRDLHNWILSEKEMECRNKRVERLVTVTASLHREMDELSIM 180
           C+DADLRSF R FDDFADSGRDLHNWIL+EKEMECRNKRVERLVTVTA+LHREMDELS+M
Sbjct: 121 CNDADLRSFHRAFDDFADSGRDLHNWILNEKEMECRNKRVERLVTVTATLHREMDELSVM 180

Query: 181 ESGLRKAVANLQSCQQENNINPPPLESSLKEQKIVDLQQKILWQRQEIKYLKEKSLWNRT 240
           E+GLRKAVANLQSCQQEN        SS  EQKI+DLQQ+I+WQRQE+KYLKEKSLWNRT
Sbjct: 181 EAGLRKAVANLQSCQQEN--------SSPLEQKIIDLQQRIVWQRQEVKYLKEKSLWNRT 240

Query: 241 FDTVMSILARSIFTTLARIKLVFGLAQFPSSLPRSLSASAAVHPLKTNLNDNGKDYCPVM 300
           FDTVMSILARS+FTTLARIK VFGL QFPSSLPRSLSASAAVHPLK NLNDNGK++ PV 
Sbjct: 241 FDTVMSILARSVFTTLARIKFVFGLGQFPSSLPRSLSASAAVHPLK-NLNDNGKEF-PV- 300

Query: 301 SKNNTFFEPNSKLLRPPPTTLGASGLGLHYANLIIVMDKMIKSPQLVGVDARDDLYSMLP 360
              N FFEPNSKLL+PP +TLGA+GL LHYANLIIVMDKMIKSPQLVGVDARDDLYSMLP
Sbjct: 301 -SKNAFFEPNSKLLKPPMSTLGATGLALHYANLIIVMDKMIKSPQLVGVDARDDLYSMLP 360

Query: 361 NSVRWALRARLRGVGFTASDPSLAGEWREAMGRILGWLSPLAQNMIKWQSERSFEQQNYM 420
           NSVR ALRARLRGVGFTASDPSLAGEWREAMGRIL WLSP+AQNMIKWQSERSFEQQNYM
Sbjct: 361 NSVRSALRARLRGVGFTASDPSLAGEWREAMGRILEWLSPMAQNMIKWQSERSFEQQNYM 420

Query: 421 APKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTAKALFACNNF 470
           APKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTAKALFACNNF
Sbjct: 421 APKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTAKALFACNNF 460

BLAST of Spg002855 vs. NCBI nr
Match: KAG6574125.1 (Protein PSK SIMULATOR 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 783.1 bits (2021), Expect = 1.3e-222
Identity = 412/473 (87.10%), Postives = 435/473 (91.97%), Query Frame = 0

Query: 1   MALETWLIKVKNAVSNKFDVVRT--STTVNVKP--TSSSSKKSPNVGVLAFEIAGLMSKL 60
           MALETWL KVKNAVSNKFD VRT  STT+N +P  TS+S+KKSPNVGVLAFEIA LMSKL
Sbjct: 1   MALETWLSKVKNAVSNKFDGVRTSSSTTLNTQPTTTSASAKKSPNVGVLAFEIAALMSKL 60

Query: 61  LHLWQSLSDNNIIRLRNESISLEGVHKIVSNDEAFLLGLACAEMTENLRLVAKSVSRLSS 120
           LHLWQSLSD +IIRLRNES+SLEGVHKIVSNDE FLLGLACAEMTENLRL+A SVSRL  
Sbjct: 61  LHLWQSLSDTHIIRLRNESLSLEGVHKIVSNDEPFLLGLACAEMTENLRLLANSVSRLCI 120

Query: 121 KCDDADLRSFERLFDDFADSGRDLHNWILSEKEMECRNKRVERLVTVTASLHREMDELSI 180
           KC+DADLRSF R FDDFADSGRDLHNWIL+EKEMECRNKRVERLVTVTA+LHREMDELS+
Sbjct: 121 KCNDADLRSFHRAFDDFADSGRDLHNWILNEKEMECRNKRVERLVTVTATLHREMDELSV 180

Query: 181 MESGLRKAVANLQSCQQENNINPPPLESSLKEQKIVDLQQKILWQRQEIKYLKEKSLWNR 240
           ME+GLRKAVANLQSCQQEN        SS  EQKI+DLQQ+I+WQRQE+KYLKEKSLWNR
Sbjct: 181 MEAGLRKAVANLQSCQQEN--------SSALEQKIIDLQQRIVWQRQEVKYLKEKSLWNR 240

Query: 241 TFDTVMSILARSIFTTLARIKLVFGLAQFPSSLPRSLSASAAVHPLKTNLNDNGKDYCPV 300
           TFDTVMSILARS+FTTLARIK VFGL QFPSSLPRSLSASAAVHPLK NLNDNGK++ PV
Sbjct: 241 TFDTVMSILARSVFTTLARIKFVFGLGQFPSSLPRSLSASAAVHPLK-NLNDNGKEF-PV 300

Query: 301 MSKNNTFFEPNSKLLRPPPTTLGASGLGLHYANLIIVMDKMIKSPQLVGVDARDDLYSML 360
               N FFEPNSKLL+PP +TLGA+GL LHYANLIIVMDKMIKSPQLVGVDARDDLYSML
Sbjct: 301 --SKNAFFEPNSKLLKPPMSTLGATGLALHYANLIIVMDKMIKSPQLVGVDARDDLYSML 360

Query: 361 PNSVRWALRARLRGVGFTASDPSLAGEWREAMGRILGWLSPLAQNMIKWQSERSFEQQNY 420
           PNSVR ALRARLRGVGFTASDPSLAGEWREAMGRIL WLSP+AQNMIKWQSERSFEQQNY
Sbjct: 361 PNSVRSALRARLRGVGFTASDPSLAGEWREAMGRILEWLSPMAQNMIKWQSERSFEQQNY 420

Query: 421 MAPKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTAKALFACNNF 470
           MAPKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTAKALFACNNF
Sbjct: 421 MAPKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTAKALFACNNF 461

BLAST of Spg002855 vs. NCBI nr
Match: XP_038898963.1 (protein PSK SIMULATOR 1 [Benincasa hispida])

HSP 1 Score: 779.2 bits (2011), Expect = 1.9e-221
Identity = 414/474 (87.34%), Postives = 436/474 (91.98%), Query Frame = 0

Query: 1   MALETWLIKVKNAVSNKFDVVRT-STTVNVKPTSSSSKKSPNVGVLAFEIAGLMSKLLHL 60
           MALETWLIKVKNAVSNKFDVVRT STTVN KPT  SSKKSPNVGVLAFEIAGLMSKLLHL
Sbjct: 1   MALETWLIKVKNAVSNKFDVVRTSSTTVNFKPT--SSKKSPNVGVLAFEIAGLMSKLLHL 60

Query: 61  WQSLSDNNIIRLRNESISLEGVHKIVSNDEAFLLGLACAEMTENLRLVAKSVSRLSSKCD 120
           W SLSD+NIIRLRNESISLEGVHKIVSND+ FLL LACAE+TENLRL+A SVSRL  KCD
Sbjct: 61  WNSLSDDNIIRLRNESISLEGVHKIVSNDDDFLLALACAEITENLRLLANSVSRLCIKCD 120

Query: 121 DADLRSFERLFDDFADSGRDLHNWILSEKEMECRNKRVERLVTVTASLHREMDELSIMES 180
             DLRSF RLF+DFADSGRDLHNWILSEKEMECRNKR+ERLVTVT++LHREMDELSIME+
Sbjct: 121 HPDLRSFHRLFNDFADSGRDLHNWILSEKEMECRNKRIERLVTVTSTLHREMDELSIMET 180

Query: 181 GLRKAVANLQSCQQENNINPPPLESSLKEQKIVDLQQKILWQRQEIKYLKEKSLWNRTFD 240
           GLRKAVA+LQSCQQ+ N      + SLKEQKI+DLQQKILWQRQE+KYLKEKSLWNRTFD
Sbjct: 181 GLRKAVASLQSCQQDQN------QISLKEQKILDLQQKILWQRQEVKYLKEKSLWNRTFD 240

Query: 241 TVMSILARSIFTTLARIKLVFGLA-QFPSSLPRSLSASAAVHPLKTNLNDNGKDYCPVMS 300
           TVMSILARSIFTTLARIKLVFGLA QFPSSLPRSLSASAAVHPLK NLNDN KD  P+  
Sbjct: 241 TVMSILARSIFTTLARIKLVFGLASQFPSSLPRSLSASAAVHPLK-NLNDNAKDSDPI-- 300

Query: 301 KNNTFFEPNSKLLRPPPTTLGASGLGLHYANLIIVMDKMIKSPQLVGVDARDDLYSMLPN 360
             N FFE NSKLL+PPPTTLGA+GL LHYANLIIVMDKMIKSPQLVGVDARDDLYSMLPN
Sbjct: 301 TKNAFFESNSKLLKPPPTTLGAAGLALHYANLIIVMDKMIKSPQLVGVDARDDLYSMLPN 360

Query: 361 SVRWALRARLRGVGFTASDPSLAGEWREAMGRILGWLSPLAQNMIKWQSERSFEQQNYMA 420
           S+R +LRARLRGVGFTASDPSLAGEWREAM RILGW+SPLAQNMIKWQSERSFEQQNY+A
Sbjct: 361 SIRSSLRARLRGVGFTASDPSLAGEWREAMERILGWMSPLAQNMIKWQSERSFEQQNYLA 420

Query: 421 PKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTAKALFACNNFNST 473
            KTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTAKALFA NNF +T
Sbjct: 421 LKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTAKALFASNNFITT 463

BLAST of Spg002855 vs. ExPASy Swiss-Prot
Match: P0DO24 (Protein PSK SIMULATOR 3 OS=Arabidopsis thaliana OX=3702 GN=PSI3 PE=1 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 2.5e-30
Identity = 115/428 (26.87%), Postives = 198/428 (46.26%), Query Frame = 0

Query: 32  TSSSSKKSPNVGVLAFEIAGLMSKLLHLWQSLSDNNIIRLRNESISLEGVHKIVSNDEAF 91
           TS  + K   +G+LAFE+A  + K  +L +SLS  NI  L+   +  EGV  +VSND   
Sbjct: 139 TSGVATKGNELGILAFEVANTIVKSSNLIESLSKRNIEHLKGTILYSEGVQNLVSNDFDE 198

Query: 92  LLGLACAEMTENLRLVAKSVSRLSSKCDDADLRSFERLFDDFADSGRDLHNWILSEKEME 151
           LL L  A+  + L++ +  V R  ++  D    + +R FD  +   ++L      +++  
Sbjct: 199 LLRLVAADKRQELQVFSGEVVRFGNRSKDFQWHNLQRYFDRIS---KELTPQRQLKEDAV 258

Query: 152 CRNKRVERLVTVTASLHREMDELSIMESGLRKAVANLQSCQQENNINPPPLESSLKEQKI 211
               ++  LV  TA L++E+  L  +E    +     +  ++EN+ N      S K   +
Sbjct: 259 LVVDQLMVLVQYTAELYQELQVLYRLEKDYEQ-----KRREEENSAN------SSKGDGL 318

Query: 212 VDLQQKILWQRQEIKYLKEKSLWNRTFDTVMSILARSIFTTLARIKLVFGLAQFPSSLPR 271
             L+ ++  QR+ +K LK+KSLW+R F+ VM  L   +   L  I  +FG          
Sbjct: 319 AILKTELKAQRKVVKSLKKKSLWSRGFEEVMEKLVDIVHFLLLEIHNIFG---------- 378

Query: 272 SLSASAAVHPLKTNLNDNGKDYCPVMSKNNTFFEPNSKLLRPPPTTLGASGLGLHYANLI 331
                             G D            +P+ K        LG +GL LHYAN+I
Sbjct: 379 ------------------GAD-----------DQPSKKGAAEYDKRLGPAGLALHYANII 438

Query: 332 IVMDKMIKSPQLVGVDARDDLYSMLPNSVRWALRARLRGVGFTASDPSLAGEWREAMGRI 391
           + +D ++     +  +ARD LY  LP  ++ ALR++++   F         + ++ M R 
Sbjct: 439 VQIDTLVARASSITSNARDSLYQSLPPGIKLALRSKIK--SFNVDKELSVTQIKDEMERT 498

Query: 392 LGWLSPLAQNMIK------WQSERSFEQQNYMAPKT--NVMLLQTLYFANKDKTEAAITE 451
           L WL P+A N  K      W  E +    ++ +  +  +++ ++TLY A+K+KTE  I  
Sbjct: 499 LHWLVPVAGNTTKAHHGFGWVGEWANTGTDFTSKPSGGDILRIETLYHASKEKTEIYILG 511

BLAST of Spg002855 vs. ExPASy Swiss-Prot
Match: Q9XID5 (Protein PSK SIMULATOR 1 OS=Arabidopsis thaliana OX=3702 GN=PSI1 PE=1 SV=2)

HSP 1 Score: 133.3 bits (334), Expect = 7.4e-30
Identity = 115/442 (26.02%), Postives = 196/442 (44.34%), Query Frame = 0

Query: 32  TSSSSKKSPNVGVLAFEIAGLMSKLLHLWQSLSDNNIIRLRNESISLEGVHKIVSNDEAF 91
           +S+++ K   + +L+FE+A  + K  +L  SLS ++I  L+   +  EGV  ++S D   
Sbjct: 145 SSATTVKGNKISILSFEVANTIVKGANLMHSLSKDSITHLKEVVLPSEGVQNLISKDMDE 204

Query: 92  LLGLACAEMTENLRLVAKSVSRLSSKCDDADLRSFERLFDDFADSGRDLHNWILSEKEME 151
           LL +A A+  E LR+ +  V R  ++C D    + +R FD     G +       ++E E
Sbjct: 205 LLRIAAADKREELRIFSGEVVRFGNRCKDPQYHNLDRFFDRL---GSEFTPQKHLKQEAE 264

Query: 152 CRNKRVERLVTVTASLHREMDELSIMESGLRKAVANLQSCQQENNINPPPLESSLKEQKI 211
               ++   V  TA L+ E+  L   E   ++ +      Q+E   NP   +  + +   
Sbjct: 265 TIMHQMMSFVHFTADLYHELHALDRFEQDYQRKI------QEEE--NPSTAQRGVGDTLA 324

Query: 212 VDLQQKILWQRQEIKYLKEKSLWNRTFDTVMSILARSIFTTLARIKLVFGLAQFPSSLPR 271
           + L+ ++  Q++ ++ LK+KSLW+R  + VM  L   +      I   FG A        
Sbjct: 325 I-LRTELKSQKKHVRNLKKKSLWSRILEEVMEKLVDVVHFLHLEIHEAFGGA-------- 384

Query: 272 SLSASAAVHPLKTNLNDNGKDYCPVMSKNNTFFEPNSKLLRPP--PTTLGASGLGLHYAN 331
                                            +P+     PP     LG++GL LHYAN
Sbjct: 385 ---------------------------------DPDKPANDPPINHKKLGSAGLALHYAN 444

Query: 332 LIIVMDKMIKSPQLVGVDARDDLYSMLPNSVRWALRARLRGVGFTASDPSLAGEWREAMG 391
           +I  +D ++     +    RD LY  LP S++ ALR+R++   F   +     + +  M 
Sbjct: 445 IITQIDTLVSRSSTMPASTRDALYQGLPPSIKSALRSRIQ--SFQVKEELTVPQIKAEME 504

Query: 392 RILGWLSPLAQNMIK------WQSE--RSFEQQNYMAPKTNVMLLQTLYFANKDKTEAAI 451
           + L WL P+A N  K      W  E   S  + N       ++ + TL+ A+K+KTEA I
Sbjct: 505 KTLQWLVPVATNTTKAHHGFGWVGEWASSGSEANQRPAGQTILRIDTLHHADKEKTEAYI 531

Query: 452 TELLVGLNYIWRFEREMTAKAL 464
            +L+V L+++    R  T   L
Sbjct: 565 LDLVVWLHHLVTQVRATTGYGL 531

BLAST of Spg002855 vs. ExPASy Swiss-Prot
Match: Q9SA91 (Protein PSK SIMULATOR 2 OS=Arabidopsis thaliana OX=3702 GN=PSI2 PE=2 SV=2)

HSP 1 Score: 123.6 bits (309), Expect = 5.8e-27
Identity = 114/435 (26.21%), Postives = 193/435 (44.37%), Query Frame = 0

Query: 35  SSKKSPNVGVLAFEIAGLMSKLLHLWQSLSDNNIIRLRNESISLEGVHKIVSNDEAFLLG 94
           +S +   V +LAFE+A  ++K   L QSLS+ N+  ++ + +  E V K+VS D   L  
Sbjct: 127 TSSRGGKVTILAFEVANTIAKGAALLQSLSEENLKFMKKDMLHSEEVKKLVSTDTTELQI 186

Query: 95  LACAEMTENLRLVAKSVSRLSSKCDDADLRSFERLFDDFADSGRDLHNWILSEKEMECRN 154
           LA ++  E L L +  V R  + C D    + +R F    D+    H   L + + E R 
Sbjct: 187 LAASDKREELDLFSGEVIRFGNMCKDLQWHNLDRYFMKL-DTENSQHK--LLKDDAEARM 246

Query: 155 KRVERLVTVTASLHREMDELSIMESGLRKAVANLQSCQQENNINPPPLESSLKEQKIVDL 214
           + +  L  +T+ L+ E+  L   E   R+ +A ++S           L    + + IV L
Sbjct: 247 QELVTLARITSELYHELQALDRFEQDYRRKLAEVES-----------LNLPRRGEGIVIL 306

Query: 215 QQKILWQRQEIKYLKEKSLWNRTFDTVMSILARSIFTTLARIKLVFGLAQFPSSLPRSLS 274
           Q ++  Q++ +K L++KSLW++    ++  L   +      I  VFG             
Sbjct: 307 QNELKQQKKLVKSLQKKSLWSQNLAEIIEKLVDVVSYIRQTIVEVFG------------- 366

Query: 275 ASAAVHPLKTNLNDNGKDYCPVMSKNNTFFEPNSKLLRPPPTTLGASGLGLHYANLIIVM 334
                + L+ N  + G++                         LG +GL LHYANLI  +
Sbjct: 367 ----NNGLRDNEGEQGRE------------------------RLGEAGLSLHYANLIQQI 426

Query: 335 DKMIKSPQLVGVDARDDLYSMLPNSVRWALRARLRGVGFTASDPSLAGEWREAMGRILGW 394
           D +   P  +  + RD LY+ LP +V+ ALR RL+ +     +     E +  M + L W
Sbjct: 427 DNIASRPSSLPSNVRDTLYNALPATVKTALRPRLQTL--DQEEELSVPEIKAEMEKSLQW 486

Query: 395 LSPLAQNMIK------WQSERSFEQQNYMAPK------TNVMLLQTLYFANKDKTEAAIT 454
           L P A+N  K      W  E +  +  +   K       N   LQTL+ A+K   ++ + 
Sbjct: 487 LVPFAENTTKAHQGFGWVGEWANSRIEFGKGKGKGENNGNPTRLQTLHHADKPIVDSYVL 504

Query: 455 ELLVGLNYIWRFERE 458
           EL+V L+ + +  ++
Sbjct: 547 ELVVWLHRLMKSSKK 504

BLAST of Spg002855 vs. ExPASy TrEMBL
Match: A0A6J1HXR5 (uncharacterized protein LOC111467797 OS=Cucurbita maxima OX=3661 GN=LOC111467797 PE=4 SV=1)

HSP 1 Score: 785.0 bits (2026), Expect = 1.7e-223
Identity = 413/475 (86.95%), Postives = 437/475 (92.00%), Query Frame = 0

Query: 1   MALETWLIKVKNAVSNKFDVVRT--STTVNVKP-TSSSSKKSPNVGVLAFEIAGLMSKLL 60
           MALETWL KVKNAVSNKFDVVRT  STT+N +P T++SSKKSPNVGVLAFEIA LMSKLL
Sbjct: 1   MALETWLSKVKNAVSNKFDVVRTSSSTTLNTQPTTTASSKKSPNVGVLAFEIAALMSKLL 60

Query: 61  HLWQSLSDNNIIRLRNESISLEGVHKIVSNDEAFLLGLACAEMTENLRLVAKSVSRLSSK 120
           HLWQSLSD +IIRLRNES+SLEGVHKIVSNDE FLLGLACAEMTENLRL+A SVSRL  K
Sbjct: 61  HLWQSLSDTHIIRLRNESLSLEGVHKIVSNDEPFLLGLACAEMTENLRLLANSVSRLCIK 120

Query: 121 CDDADLRSFERLFDDFADSGRDLHNWILSEKEMECRNKRVERLVTVTASLHREMDELSIM 180
           C+DADLRSF R FDDFADSGRDLHNWIL EKEMECRNKRVERLVTVTA+LH+EMDELS+M
Sbjct: 121 CNDADLRSFHRAFDDFADSGRDLHNWILKEKEMECRNKRVERLVTVTATLHKEMDELSVM 180

Query: 181 ESGLRKAVANLQSCQQENNINPPPLESSLKEQKIVDLQQKILWQRQEIKYLKEKSLWNRT 240
           E+GLRKAVANLQSCQQEN        SS  EQKI+DLQQ+I+WQRQE+KYLKEKSLWNRT
Sbjct: 181 EAGLRKAVANLQSCQQEN--------SSPLEQKIIDLQQRIVWQRQEVKYLKEKSLWNRT 240

Query: 241 FDTVMSILARSIFTTLARIKLVFGLAQFPSSLPRSLSASAAVHPLKTNLNDNGKDYCPVM 300
           FDTVMSILARS+FTTLARIK VFGL QFPSSLPRSLSASAAVHPLK NLNDNGK++ PV 
Sbjct: 241 FDTVMSILARSVFTTLARIKFVFGLGQFPSSLPRSLSASAAVHPLK-NLNDNGKEF-PV- 300

Query: 301 SKNNTFFEPNSKLLRPPPTTLGASGLGLHYANLIIVMDKMIKSPQLVGVDARDDLYSMLP 360
              N FFEPNSKLL+PP +TLGA+GL LHYANLIIVMDKMIKSPQLVGVDARDDLYSMLP
Sbjct: 301 -SKNAFFEPNSKLLKPPMSTLGATGLALHYANLIIVMDKMIKSPQLVGVDARDDLYSMLP 360

Query: 361 NSVRWALRARLRGVGFTASDPSLAGEWREAMGRILGWLSPLAQNMIKWQSERSFEQQNYM 420
           NSVR ALRARLRGVGFTASDPSLAGEWREAMGRIL WLSP+AQNMIKWQSERSFEQQNYM
Sbjct: 361 NSVRSALRARLRGVGFTASDPSLAGEWREAMGRILEWLSPMAQNMIKWQSERSFEQQNYM 420

Query: 421 APKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTAKALFACNNFNST 473
           APKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTAKALFACNNF +T
Sbjct: 421 APKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTAKALFACNNFIAT 463

BLAST of Spg002855 vs. ExPASy TrEMBL
Match: A0A6J1G2G2 (uncharacterized protein LOC111450042 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450042 PE=4 SV=1)

HSP 1 Score: 784.3 bits (2024), Expect = 2.9e-223
Identity = 412/472 (87.29%), Postives = 436/472 (92.37%), Query Frame = 0

Query: 1   MALETWLIKVKNAVSNKFDVVRTS--TTVNVKP-TSSSSKKSPNVGVLAFEIAGLMSKLL 60
           MALETWL KVKNAVSNKFDVVRTS  TT+N +P T++S+KKSPNVGVLAFEIA LMSKLL
Sbjct: 1   MALETWLSKVKNAVSNKFDVVRTSSLTTLNTQPTTTASTKKSPNVGVLAFEIAALMSKLL 60

Query: 61  HLWQSLSDNNIIRLRNESISLEGVHKIVSNDEAFLLGLACAEMTENLRLVAKSVSRLSSK 120
           HLWQSLSD +IIRLRNES+SLEGVHKIVSNDE FLLGLACAEMTENLRL+A SVSRL  K
Sbjct: 61  HLWQSLSDTHIIRLRNESLSLEGVHKIVSNDEPFLLGLACAEMTENLRLLANSVSRLCIK 120

Query: 121 CDDADLRSFERLFDDFADSGRDLHNWILSEKEMECRNKRVERLVTVTASLHREMDELSIM 180
           C+DADLRSF R FDDFADSGRDLHNWIL+EKEMECRNKRVERLVTVTA+LHREMDELS+M
Sbjct: 121 CNDADLRSFHRAFDDFADSGRDLHNWILNEKEMECRNKRVERLVTVTATLHREMDELSVM 180

Query: 181 ESGLRKAVANLQSCQQENNINPPPLESSLKEQKIVDLQQKILWQRQEIKYLKEKSLWNRT 240
           E+GLRKAVANLQSCQQEN        SS  EQKI+DLQQ+I+WQRQE+KYLKEKSLWNRT
Sbjct: 181 EAGLRKAVANLQSCQQEN--------SSPLEQKIIDLQQRIVWQRQEVKYLKEKSLWNRT 240

Query: 241 FDTVMSILARSIFTTLARIKLVFGLAQFPSSLPRSLSASAAVHPLKTNLNDNGKDYCPVM 300
           FDTVMSILARS+FTTLARIK VFGL QFPSSLPRSLSASAAVHPLK NLNDNGK++ PV 
Sbjct: 241 FDTVMSILARSVFTTLARIKFVFGLGQFPSSLPRSLSASAAVHPLK-NLNDNGKEF-PV- 300

Query: 301 SKNNTFFEPNSKLLRPPPTTLGASGLGLHYANLIIVMDKMIKSPQLVGVDARDDLYSMLP 360
              N FFEPNSKLL+PP +TLGA+GL LHYANLIIVMDKMIKSPQLVGVDARDDLYSMLP
Sbjct: 301 -SKNAFFEPNSKLLKPPMSTLGATGLALHYANLIIVMDKMIKSPQLVGVDARDDLYSMLP 360

Query: 361 NSVRWALRARLRGVGFTASDPSLAGEWREAMGRILGWLSPLAQNMIKWQSERSFEQQNYM 420
           NSVR ALRARLRGVGFTASDPSLAGEWREAMGRIL WLSP+AQNMIKWQSERSFEQQNYM
Sbjct: 361 NSVRSALRARLRGVGFTASDPSLAGEWREAMGRILEWLSPMAQNMIKWQSERSFEQQNYM 420

Query: 421 APKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTAKALFACNNF 470
           APKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTAKALFACNNF
Sbjct: 421 APKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTAKALFACNNF 460

BLAST of Spg002855 vs. ExPASy TrEMBL
Match: A0A6J1CJ19 (uncharacterized protein LOC111011445 OS=Momordica charantia OX=3673 GN=LOC111011445 PE=4 SV=1)

HSP 1 Score: 778.9 bits (2010), Expect = 1.2e-221
Identity = 410/477 (85.95%), Postives = 439/477 (92.03%), Query Frame = 0

Query: 1   MALETWLIKVKNAVSNKFDVVRTSTT---VNVKPTSSSS-KKSPNVGVLAFEIAGLMSKL 60
           MALET L+KVKNAVSN+FD VRTS+T    N+KPTSSSS KKS NVGVLAFEIAGLMSKL
Sbjct: 1   MALETLLVKVKNAVSNRFDAVRTSSTAAAANIKPTSSSSKKKSANVGVLAFEIAGLMSKL 60

Query: 61  LHLWQSLSDNNIIRLRNESISLEGVHKIVSNDEAFLLGLACAEMTENLRLVAKSVSRLSS 120
           LHLWQSLSD NI+RLRNES+SLEGVHKIVSND+AFLLGLACAE+T+NLRL+A SVSR +S
Sbjct: 61  LHLWQSLSDANIVRLRNESVSLEGVHKIVSNDDAFLLGLACAEITQNLRLLANSVSRFTS 120

Query: 121 KCDDADLRSFERLFDDFADSGRDLHNWILSEKEMECRNKRVERLVTVTASLHREMDELSI 180
           KCDDA LRSF+R F DFADSGRDLHNW LSEKEMECRNKRVERLVTVTA+LHREMDEL+I
Sbjct: 121 KCDDATLRSFDRFFHDFADSGRDLHNWTLSEKEMECRNKRVERLVTVTATLHREMDELTI 180

Query: 181 MESGLRKAVANLQSCQQENNINPPPLESSLKEQKIVDLQQKILWQRQEIKYLKEKSLWNR 240
           ME+GLRKAVANLQSCQQE + N P LE+SLKEQKI+DLQ KILWQRQE+KYLKEKSLWNR
Sbjct: 181 METGLRKAVANLQSCQQEIS-NNPSLETSLKEQKILDLQHKILWQRQEVKYLKEKSLWNR 240

Query: 241 TFDTVMSILARSIFTTLARIKLVFGLAQFPSSLPRSLSASAAVHPLKTNLNDNGKDYCPV 300
           TFDT +SILARSIFTTLARIKLVFGLAQFPSSLPR LSASAAVHPLK NL + GKD+   
Sbjct: 241 TFDTAVSILARSIFTTLARIKLVFGLAQFPSSLPRCLSASAAVHPLK-NLTETGKDF--- 300

Query: 301 MSKNNTFFEPNSKLLRPPPTTLGASGLGLHYANLIIVMDKMIKSPQLVGVDARDDLYSML 360
           +   N FFE NSKLL+PPP+TLGA+GL LHYANLIIVMDKMIKSPQLVGVDARDDLYSML
Sbjct: 301 LVTKNAFFESNSKLLKPPPSTLGAAGLALHYANLIIVMDKMIKSPQLVGVDARDDLYSML 360

Query: 361 PNSVRWALRARLRGVGFTASDPSLAGEWREAMGRILGWLSPLAQNMIKWQSERSFEQQNY 420
           PNSVR ALR RLRGVGFTASDPSLAGEWREA+GRILGWLSPLAQNM+KWQSERSFEQQNY
Sbjct: 361 PNSVRSALRGRLRGVGFTASDPSLAGEWREALGRILGWLSPLAQNMMKWQSERSFEQQNY 420

Query: 421 -MAPKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTAKALFACNNFNST 473
            MAPKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTAKALFACNNF S+
Sbjct: 421 NMAPKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTAKALFACNNFISS 472

BLAST of Spg002855 vs. ExPASy TrEMBL
Match: A0A5D3E450 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1044G00020 PE=4 SV=1)

HSP 1 Score: 778.5 bits (2009), Expect = 1.6e-221
Identity = 412/472 (87.29%), Postives = 434/472 (91.95%), Query Frame = 0

Query: 1   MALETWLIKVKNAVSNKFDVVR-TSTTVNVKPTSSSSKKSPNVGVLAFEIAGLMSKLLHL 60
           MALETWLIKVKNAVSNKFDVVR +STT N KPT  SSKKSPNV VL+FEIAGLMSKLLHL
Sbjct: 1   MALETWLIKVKNAVSNKFDVVRASSTTPNFKPT--SSKKSPNVAVLSFEIAGLMSKLLHL 60

Query: 61  WQSLSDNNIIRLRNESISLEGVHKIVSNDEAFLLGLACAEMTENLRLVAKSVSRLSSKCD 120
           W SLSD+NI RLRN+SISLEGVHKIVSND+ FLL LACAE+TENLRL+A SVS L  KCD
Sbjct: 61  WNSLSDHNITRLRNQSISLEGVHKIVSNDDDFLLALACAEITENLRLLANSVSPLCIKCD 120

Query: 121 DADLRSFERLFDDFADSGRDLHNWILSEKEMECRNKRVERLVTVTASLHREMDELSIMES 180
             DLRSF RLF +FADSGRDLHNWILSEKEMECRNKR+ERLVT+TA+LHREMDELSIME+
Sbjct: 121 HPDLRSFHRLFLEFADSGRDLHNWILSEKEMECRNKRIERLVTMTANLHREMDELSIMET 180

Query: 181 GLRKAVANLQSCQQE-NNINPPPLESSLKEQKIVDLQQKILWQRQEIKYLKEKSLWNRTF 240
           GLRKAVANLQ CQQE NN + PPLE SLKEQKI+DLQQKILWQRQE+KYLKEKSLWN+TF
Sbjct: 181 GLRKAVANLQLCQQEQNNSSTPPLEISLKEQKILDLQQKILWQRQEVKYLKEKSLWNKTF 240

Query: 241 DTVMSILARSIFTTLARIKLVFGLA-QFPSSLPRSLSASAAVHPLKTNLNDNGKDYCPVM 300
           DTV+SILARSIFTTLARIKLVFGLA QFPSSLPRSLSASAAVHPLK NLNDN KD  P  
Sbjct: 241 DTVISILARSIFTTLARIKLVFGLAHQFPSSLPRSLSASAAVHPLK-NLNDNAKDSDPTT 300

Query: 301 SKNNTFFEPNSKLLRPPPTTLGASGLGLHYANLIIVMDKMIKSPQLVGVDARDDLYSMLP 360
           +KN  FFE N KLL+PPPTTLGA+GL LHYANLIIVMDKMIKSPQLVGVDARDDLYSMLP
Sbjct: 301 TKNG-FFESNLKLLKPPPTTLGAAGLALHYANLIIVMDKMIKSPQLVGVDARDDLYSMLP 360

Query: 361 NSVRWALRARLRGVGFTASDPSLAGEWREAMGRILGWLSPLAQNMIKWQSERSFEQQNYM 420
           NSVR +LRARLRGVGFTASD SLAGEWREAMGRILGW+SPLAQNMIKWQSERSFEQQNYM
Sbjct: 361 NSVRTSLRARLRGVGFTASDASLAGEWREAMGRILGWMSPLAQNMIKWQSERSFEQQNYM 420

Query: 421 APKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTAKALFACNNF 470
           APKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTA ALFACNNF
Sbjct: 421 APKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTANALFACNNF 468

BLAST of Spg002855 vs. ExPASy TrEMBL
Match: A0A1S3AYM2 (uncharacterized protein LOC103484007 OS=Cucumis melo OX=3656 GN=LOC103484007 PE=4 SV=1)

HSP 1 Score: 776.9 bits (2005), Expect = 4.7e-221
Identity = 411/472 (87.08%), Postives = 433/472 (91.74%), Query Frame = 0

Query: 1   MALETWLIKVKNAVSNKFDVVR-TSTTVNVKPTSSSSKKSPNVGVLAFEIAGLMSKLLHL 60
           MALETWLIKVKNAVSNKFDVVR +STT N KPT  SSKKSPNV VL+FEIAGLMSKLLHL
Sbjct: 1   MALETWLIKVKNAVSNKFDVVRASSTTPNFKPT--SSKKSPNVAVLSFEIAGLMSKLLHL 60

Query: 61  WQSLSDNNIIRLRNESISLEGVHKIVSNDEAFLLGLACAEMTENLRLVAKSVSRLSSKCD 120
           W SLSD+NI RLRN+SISLEGVHKIVSND+ FLL LACAE+TENLRL+A SVS L  KCD
Sbjct: 61  WNSLSDHNITRLRNQSISLEGVHKIVSNDDDFLLALACAEITENLRLLANSVSPLCIKCD 120

Query: 121 DADLRSFERLFDDFADSGRDLHNWILSEKEMECRNKRVERLVTVTASLHREMDELSIMES 180
             DLRSF RLF +FADSGRDLHNWILSEKEMECRNKR+ERLVT+TA+LHREMDELSIME+
Sbjct: 121 HPDLRSFHRLFLEFADSGRDLHNWILSEKEMECRNKRIERLVTMTANLHREMDELSIMET 180

Query: 181 GLRKAVANLQSCQQE-NNINPPPLESSLKEQKIVDLQQKILWQRQEIKYLKEKSLWNRTF 240
           GLRKAV NLQ CQQE NN + PPLE SLKEQKI+DLQQKILWQRQE+KYLKEKSLWN+TF
Sbjct: 181 GLRKAVTNLQLCQQEQNNSSTPPLEISLKEQKILDLQQKILWQRQEVKYLKEKSLWNKTF 240

Query: 241 DTVMSILARSIFTTLARIKLVFGLA-QFPSSLPRSLSASAAVHPLKTNLNDNGKDYCPVM 300
           DTV+SILARSIFTTLARIKLVFGLA QFPSSLPRSLSASAAVHPLK NLNDN KD  P  
Sbjct: 241 DTVISILARSIFTTLARIKLVFGLAHQFPSSLPRSLSASAAVHPLK-NLNDNAKDSDPTT 300

Query: 301 SKNNTFFEPNSKLLRPPPTTLGASGLGLHYANLIIVMDKMIKSPQLVGVDARDDLYSMLP 360
           +KN  FFE N KLL+PPPTTLGA+GL LHYANLIIVMDKMIKSPQLVGVDARDDLYSMLP
Sbjct: 301 TKNG-FFESNLKLLKPPPTTLGAAGLALHYANLIIVMDKMIKSPQLVGVDARDDLYSMLP 360

Query: 361 NSVRWALRARLRGVGFTASDPSLAGEWREAMGRILGWLSPLAQNMIKWQSERSFEQQNYM 420
           NSVR +LRARLRGVGFTASD SLAGEWREAMGRILGW+SPLAQNMIKWQSERSFEQQNYM
Sbjct: 361 NSVRTSLRARLRGVGFTASDASLAGEWREAMGRILGWMSPLAQNMIKWQSERSFEQQNYM 420

Query: 421 APKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTAKALFACNNF 470
           APKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTA ALFACNNF
Sbjct: 421 APKTNVMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTANALFACNNF 468

BLAST of Spg002855 vs. TAIR 10
Match: AT5G51670.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 478.4 bits (1230), Expect = 6.6e-135
Identity = 271/480 (56.46%), Postives = 351/480 (73.12%), Query Frame = 0

Query: 1   MALETWLIKVKNAVSNKFDVVRTSTTVNVKPTSSSSKKSPNVGVLAFEIAGLMSKLLHLW 60
           MALET+LIK+KNA+S+K    R   +     T++SS     VGVL+FE+A +M+KLLHL 
Sbjct: 1   MALETFLIKLKNAISSKPTSRRPHRSSPPISTTTSS-----VGVLSFEVARVMTKLLHLT 60

Query: 61  QSLSDNNIIRLRNESISLEGVHKIVSNDEAFLLGLACAEMTENLRLVAKSVSRLSSKCDD 120
            SL+D+N++  R+ S+SLEG+ KIV+ DE F L L CAE+ ++L   A SVSRLS++C  
Sbjct: 61  HSLTDSNLLTPRDHSLSLEGLTKIVNGDETFHLSLVCAELADSLAHAANSVSRLSNRCTT 120

Query: 121 ADLRSFERLFDDFADSGRDLHNWILSEKEMECRNKRVERLVTVTASLHREMDELSIMESG 180
           A LRSF RLF +FAD GRD H W+++ K+ E +NK++ER V+VT +L+REM+E++I+E+ 
Sbjct: 121 ASLRSFHRLFHEFADMGRDPHGWVMNCKDTEAKNKKIERYVSVTTALYREMEEMAILENS 180

Query: 181 LRKAVANLQSCQQENNINPPPLESSLKEQKIVDLQQKILWQRQEIKYLKEKSLWNRTFDT 240
           LRK    +    +E        E+     K++DLQ KI  Q+Q +KYLK++SLWN++FDT
Sbjct: 181 LRKQSLQIGIEFEEEE----DYENKKDVMKVIDLQNKIERQKQHVKYLKDRSLWNKSFDT 240

Query: 241 VMSILARSIFTTLARIKLVFGLA--------QFPSSLPRSLSASAA----VHPLKTNLND 300
           V+ ILARS+FT LAR+K VF  A           SSLPRSLS+S++    VHP   + ND
Sbjct: 241 VVLILARSVFTALARLKSVFSSAAATGYMVPTVVSSLPRSLSSSSSSMNLVHP---SPND 300

Query: 301 NGKDYCPVMSKNNTFFEPNSKLLRPPPTTLGASGLGLHYANLIIVMDKMIKSPQLVGVDA 360
             +D     + ++ F E +S+LL+PP TTLG +G+ LHYANLI+VM+KMIK PQLVG+DA
Sbjct: 301 EERD---KTTTSSAFLEESSRLLKPPETTLGGAGVALHYANLIVVMEKMIKQPQLVGLDA 360

Query: 361 RDDLYSMLPNSVRWALRARLRGVGFTASDPSLAGEWREAMGRILGWLSPLAQNMIKWQSE 420
           RDDLYSMLP SVR +LR+RL+GVGFTA+D  LA EW+ A+GRIL WL PLAQNMI+WQSE
Sbjct: 361 RDDLYSMLPASVRSSLRSRLKGVGFTATDGGLATEWKAALGRILRWLLPLAQNMIRWQSE 420

Query: 421 RSFEQQNYMAPKTN----VMLLQTLYFANKDKTEAAITELLVGLNYIWRFEREMTAKALF 465
           RSFEQQ +MA  TN    VML+QTL FA+K KTEAAITELLVGLNYIWRFEREMTAKALF
Sbjct: 421 RSFEQQ-HMATATNSQNRVMLVQTLVFADKVKTEAAITELLVGLNYIWRFEREMTAKALF 464

BLAST of Spg002855 vs. TAIR 10
Match: AT3G23160.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 307.0 bits (785), Expect = 2.6e-83
Identity = 195/523 (37.28%), Postives = 291/523 (55.64%), Query Frame = 0

Query: 1   MALETWLIKVKNAVSN--KFDVVRTSTTVNVKPTSSSSKKSPNVGVLAFEIAGLMSKLLH 60
           M  E W++K++N VS+  K  ++  S++    P   S  K   +G+L+FE+A +MSK +H
Sbjct: 1   MVSEAWIVKMRNQVSSNLKHALLLESSSTKKTPKPVSLPKQ-TIGILSFEVANVMSKTIH 60

Query: 61  LWQSLSDNNIIRLRNESISLEGVHKIVSNDEAFLLGLACAEMTENLRLVAKSVSRLSSKC 120
           L +SLSD  I +L+ E    EGV K+VS+DE  LL L+ +E  ++L  VA  VSRL  KC
Sbjct: 61  LHRSLSDTEISKLKAEVFHSEGVRKLVSSDENHLLDLSVSEKLDDLSRVASVVSRLGKKC 120

Query: 121 DDADLRSFERLFDDFADSGRDLHNWILSEKEMECRNKRVERLVTVTASLHREMDELSIME 180
           ++  L+ FE +++D  +   D        K+ME   K++ER V  T SL+ EM+ ++ +E
Sbjct: 121 NEPALQGFEHVYEDIVNGAIDFRKLGFLVKDMESMVKKMERFVNATCSLYCEMEVMNELE 180

Query: 181 SGLRKAVANLQSCQQENNINPPPLESSLKEQKIVDLQQKILWQRQEIKYLKEKSLWNRTF 240
               +A+  LQ  QQ              ++ +   +QK++WQRQ++K L++ SLWN+T+
Sbjct: 181 ----QAIVKLQRSQQH-------------QESVKAFEQKLMWQRQDVKSLRDGSLWNQTY 240

Query: 241 DTVMSILARSIFTTLARIKLVFG----LAQFPSSLPRSLS---ASAAVH----------- 300
           D V+ +LAR++ T   RI+ VFG      +   +L R  S   AS AV+           
Sbjct: 241 DKVVEMLARTVCTIYGRIETVFGGLGLRGKKDVTLKRDRSKNEASKAVNSRSVAGFKDSR 300

Query: 301 ---------------PLKTNL----------------------NDNGKDYCPV----MSK 360
                          P  TN                        D G+   P+    M +
Sbjct: 301 RSEADEFTRAGDFNFPCGTNPGRMFMECLAMNRTIGDDDDDDDEDGGRITFPLSTARMIR 360

Query: 361 NNTFFEPNSKLLRPPPTTLGASGLGLHYANLIIVMDKMIKSPQLVGVDARDDLYSMLPNS 420
           +N F   +        +T+G S L LHYAN++IV++K++K P L+G +ARDDLY MLP S
Sbjct: 361 SNKFGFKSRLTQHASASTIGGSALSLHYANVVIVVEKLLKYPHLIGEEARDDLYQMLPTS 420

Query: 421 VRWALRARLRGV--GFTASDPSLAGEWREAMGRILGWLSPLAQNMIKWQSERSFEQQNYM 461
           ++  L+A LR      +  D  LA +W+E +  IL WL+PLA NMI+WQSER+FEQQN +
Sbjct: 421 LKTTLKASLRSYLKNISIYDAPLAHDWKETIDGILSWLAPLAHNMIRWQSERNFEQQNQI 480

BLAST of Spg002855 vs. TAIR 10
Match: AT5G04550.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 300.8 bits (769), Expect = 1.9e-81
Identity = 204/580 (35.17%), Postives = 283/580 (48.79%), Query Frame = 0

Query: 42  VGVLAFEIAGLMSKLLHLWQSLSDNNIIRLRNESISLEGVHKIVSNDEAFLLGLACAEMT 101
           +GVLAFE+A L+SKL+HLWQSLSD N+ RLR+E     G+ K+VS D+ F++ L   EM 
Sbjct: 26  LGVLAFEVASLLSKLVHLWQSLSDKNVARLRDEITHSTGIKKLVSEDDDFIVRLIRDEMM 85

Query: 102 ENLRLVAKSVSRLSSKCDDADLRSFERLFDDFADSGRDLHNWILSEKEMECRNKRVERLV 161
           EN+  VAK+V+RL+ KC+D  L+ FE  F D   +G D + W    K+M+ + K++ER +
Sbjct: 86  ENVENVAKAVARLARKCNDPKLKCFENCFSDMMKTGADPYGWQFGWKKMDKKAKKMERFI 145

Query: 162 TVTASLHREMDELSIMESGLRKAVANLQSCQQENNINPPPLESSLKEQKIVDLQQKILWQ 221
           +  ASL++E + L+ +E   ++  +N               ES+     +++ Q+K+ W+
Sbjct: 146 SSNASLYQETEILADLEQTFKRMKSN---------------ESA--TDNLLEYQKKVTWK 205

Query: 222 RQEIKYLKEKSLWNRTFDTVMSILARSIFTTLARIKLVFGLA------------------ 281
           R E+K L++ SLWNRT+D  + +L RS+FT L+R K VFG++                  
Sbjct: 206 RHEVKNLRDVSLWNRTYDYTVILLVRSVFTILSRTKHVFGISYRVEASDVSSADSDFIGR 265

Query: 282 --------------QFPSSLPR------------------------------SLSASA-- 341
                            S LPR                              SLSA +  
Sbjct: 266 SHSVSTILTPVSHKSESSGLPRFASGPLGRFTGPASGSAATRSTKMSDFLSGSLSAESPK 325

Query: 342 ------------------------------------------------------------ 401
                                                                       
Sbjct: 326 SGPLVAEKHKRFKFYSGQLGKITSKSGPLIGMGKNNKKMGQTPERPSISSVKKQLKANRL 385

Query: 402 -AVHPLK---------TNLNDNGKDYCPVMSKNNTFFEPNS--------------KLLRP 461
             V P K         T L+   ++     S  +   E NS              KL   
Sbjct: 386 TQVGPFKGCMVSHDGITPLSTRARNGARNSSAEHHILEDNSNSVHVENLTLPSRPKLSDA 445

Query: 462 PPTTLGASGLGLHYANLIIVMDKMIKSPQLVGVDARDDLYSMLPNSVRWALRARLRGV-- 469
            P TLG + L LHYAN+IIV+++ + SP L+G DARDDLY+MLP SVR +LR RL+    
Sbjct: 446 APNTLGTACLALHYANVIIVIERFVASPHLIGDDARDDLYNMLPASVRTSLRERLKPYSK 505

BLAST of Spg002855 vs. TAIR 10
Match: AT5G08660.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 134.8 bits (338), Expect = 1.8e-31
Identity = 115/428 (26.87%), Postives = 198/428 (46.26%), Query Frame = 0

Query: 32  TSSSSKKSPNVGVLAFEIAGLMSKLLHLWQSLSDNNIIRLRNESISLEGVHKIVSNDEAF 91
           TS  + K   +G+LAFE+A  + K  +L +SLS  NI  L+   +  EGV  +VSND   
Sbjct: 139 TSGVATKGNELGILAFEVANTIVKSSNLIESLSKRNIEHLKGTILYSEGVQNLVSNDFDE 198

Query: 92  LLGLACAEMTENLRLVAKSVSRLSSKCDDADLRSFERLFDDFADSGRDLHNWILSEKEME 151
           LL L  A+  + L++ +  V R  ++  D    + +R FD  +   ++L      +++  
Sbjct: 199 LLRLVAADKRQELQVFSGEVVRFGNRSKDFQWHNLQRYFDRIS---KELTPQRQLKEDAV 258

Query: 152 CRNKRVERLVTVTASLHREMDELSIMESGLRKAVANLQSCQQENNINPPPLESSLKEQKI 211
               ++  LV  TA L++E+  L  +E    +     +  ++EN+ N      S K   +
Sbjct: 259 LVVDQLMVLVQYTAELYQELQVLYRLEKDYEQ-----KRREEENSAN------SSKGDGL 318

Query: 212 VDLQQKILWQRQEIKYLKEKSLWNRTFDTVMSILARSIFTTLARIKLVFGLAQFPSSLPR 271
             L+ ++  QR+ +K LK+KSLW+R F+ VM  L   +   L  I  +FG          
Sbjct: 319 AILKTELKAQRKVVKSLKKKSLWSRGFEEVMEKLVDIVHFLLLEIHNIFG---------- 378

Query: 272 SLSASAAVHPLKTNLNDNGKDYCPVMSKNNTFFEPNSKLLRPPPTTLGASGLGLHYANLI 331
                             G D            +P+ K        LG +GL LHYAN+I
Sbjct: 379 ------------------GAD-----------DQPSKKGAAEYDKRLGPAGLALHYANII 438

Query: 332 IVMDKMIKSPQLVGVDARDDLYSMLPNSVRWALRARLRGVGFTASDPSLAGEWREAMGRI 391
           + +D ++     +  +ARD LY  LP  ++ ALR++++   F         + ++ M R 
Sbjct: 439 VQIDTLVARASSITSNARDSLYQSLPPGIKLALRSKIK--SFNVDKELSVTQIKDEMERT 498

Query: 392 LGWLSPLAQNMIK------WQSERSFEQQNYMAPKT--NVMLLQTLYFANKDKTEAAITE 451
           L WL P+A N  K      W  E +    ++ +  +  +++ ++TLY A+K+KTE  I  
Sbjct: 499 LHWLVPVAGNTTKAHHGFGWVGEWANTGTDFTSKPSGGDILRIETLYHASKEKTEIYILG 511

BLAST of Spg002855 vs. TAIR 10
Match: AT1G34320.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 133.3 bits (334), Expect = 5.2e-31
Identity = 115/442 (26.02%), Postives = 196/442 (44.34%), Query Frame = 0

Query: 32  TSSSSKKSPNVGVLAFEIAGLMSKLLHLWQSLSDNNIIRLRNESISLEGVHKIVSNDEAF 91
           +S+++ K   + +L+FE+A  + K  +L  SLS ++I  L+   +  EGV  ++S D   
Sbjct: 145 SSATTVKGNKISILSFEVANTIVKGANLMHSLSKDSITHLKEVVLPSEGVQNLISKDMDE 204

Query: 92  LLGLACAEMTENLRLVAKSVSRLSSKCDDADLRSFERLFDDFADSGRDLHNWILSEKEME 151
           LL +A A+  E LR+ +  V R  ++C D    + +R FD     G +       ++E E
Sbjct: 205 LLRIAAADKREELRIFSGEVVRFGNRCKDPQYHNLDRFFDRL---GSEFTPQKHLKQEAE 264

Query: 152 CRNKRVERLVTVTASLHREMDELSIMESGLRKAVANLQSCQQENNINPPPLESSLKEQKI 211
               ++   V  TA L+ E+  L   E   ++ +      Q+E   NP   +  + +   
Sbjct: 265 TIMHQMMSFVHFTADLYHELHALDRFEQDYQRKI------QEEE--NPSTAQRGVGDTLA 324

Query: 212 VDLQQKILWQRQEIKYLKEKSLWNRTFDTVMSILARSIFTTLARIKLVFGLAQFPSSLPR 271
           + L+ ++  Q++ ++ LK+KSLW+R  + VM  L   +      I   FG A        
Sbjct: 325 I-LRTELKSQKKHVRNLKKKSLWSRILEEVMEKLVDVVHFLHLEIHEAFGGA-------- 384

Query: 272 SLSASAAVHPLKTNLNDNGKDYCPVMSKNNTFFEPNSKLLRPP--PTTLGASGLGLHYAN 331
                                            +P+     PP     LG++GL LHYAN
Sbjct: 385 ---------------------------------DPDKPANDPPINHKKLGSAGLALHYAN 444

Query: 332 LIIVMDKMIKSPQLVGVDARDDLYSMLPNSVRWALRARLRGVGFTASDPSLAGEWREAMG 391
           +I  +D ++     +    RD LY  LP S++ ALR+R++   F   +     + +  M 
Sbjct: 445 IITQIDTLVSRSSTMPASTRDALYQGLPPSIKSALRSRIQ--SFQVKEELTVPQIKAEME 504

Query: 392 RILGWLSPLAQNMIK------WQSE--RSFEQQNYMAPKTNVMLLQTLYFANKDKTEAAI 451
           + L WL P+A N  K      W  E   S  + N       ++ + TL+ A+K+KTEA I
Sbjct: 505 KTLQWLVPVATNTTKAHHGFGWVGEWASSGSEANQRPAGQTILRIDTLHHADKEKTEAYI 531

Query: 452 TELLVGLNYIWRFEREMTAKAL 464
            +L+V L+++    R  T   L
Sbjct: 565 LDLVVWLHHLVTQVRATTGYGL 531