Spg002643 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg002643
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 like
Location	scaffold6: 1107793 .. 1109445 (-)
RNA-Seq Expression	Spg002643
Synteny	Spg002643

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGGGAAGAGAGAGAGAGAGTCCTCTTCATTCTCCCAAAGCGCCTGGCTTGTCACGACTGAGAGAGTGATCAAGAGAGAGAGAGAGACAGAGGAGTTTTCCGACGATGTTTCTGCGAGTGATCGGACGGCCATTACTGGCCAGGGTGAAGCAAACGACGGGGATCGTCGGTCTGGATGTCGTCCCCAACGCCCGAGAGGTCTTGATCGGCCTTTACAATAAAACCCTAAAGGAGATCCAGGCCGTTCCCGAGGACGAAGGATACCGTAAGGCGGTCGAGAGCTTCACACGCCACCGCTTGAAGGTCTGCCAGGAGGAAGAGGACTGGGAGCAAATTGAGAAGCGTCTTGGCTGTGGTCAGGTCGAGGAGCTCATCGAGGAGGCTCAGGATGAGCTCAAGCTCATTGGGAAAATGATCGGTATGTATATAAATTTATGTAGCCAAAGTTCTATCTTTACGTATTTGTGAAACTTTGATGTACAGTCGAGTTCCATTACATTCTTTCCATGTCTGTTTCGGTTTAGGAGGGGAATGTATGAATTGGAGATTCAAGTTAGCAGGATTTTCGAGTTATATGATTTTTATATTCTTGCATTGCGTGGAAATGATGAAAACCGTACTTTTCCTGATCAAATTTCTAAGGAAAAATCGTGACTGCGAGTTTTAGAAATGCTGGTTATTATGAGTCTGATATCGTTCATGGGAACTCGTAGGGAAAAGAACGGAGGTTCACGATTTCTCTAGTACTGAGTGTCAAAGAATGGCATTTGCATCTTGTAGCACTGGAAAAAGCTGGGTTGAGAGTAGGAATTAGACTGTAATGATCCTTCTGCCGTTGTTAGCTTCTTACCTCGTTGAATAAAATGACGAGCCTCATGGCCTAATAGCAATCCCTGATTTGTTTTCGTCTCTTAAACCGATTCGTGTCTCTGAACTTTTGAAATTGATGTTCTATTCTAACTGAGGTGGATGAAAGTTACATATTTCGACATTAGTTTAACATATCAAGCTGAAGCATCATGAAGGAGGATATATATGTATGTATTTGGTCAAGTAATAGGCTTGACTTATTACTTGACTCTTCTGGTCTTGTTGCATTGGCGCATGCTCATTATTTCAAAGATGGAACTTTATAAAATTTTGTCAGTCATCGCAATCTTTTTGTGGTTTTGCTAGTGATTTGTTATCTGAATTATGCAGAGTGGGATCCCTGGGGTGTTCCTGATGACTACGAATGTGAAGTGATCGAGAACGATGCTCCGGTACCCAAGCACATTCCTCTGCATCGACCAGGCCCTCTCCCTGAGGAGTTTTACAAAACGCTGGAGGCCATTAGTGGTGGTAGTGCACAAAAAGTAGAGACGGCCGAGAAAGCATCGGTGACAGAGTGACCCTTTCTTAACCATTTGGAGGATTCGAGTTTCAGAATCGAAAGCCGTTCTTTGTTTTTGGCCTTGATGTTATCTCATTATGTTTGTTCTCCAATAGAGTGCCTGTATGCAGGAATGATTGTTCACAAAGTCGTGTACTTCAATAATCTTTACCCAATTCTGGAATTACATAAATTCATCTTTTCATTACCTTGTTTAAATCTTACTCTGCGCATCAATCCAGTCATGAATGGATTTTGAGACTGAGCAATTTTAATCCTTTC

mRNA sequence

ATGTTTCTGCGAGTGATCGGACGGCCATTACTGGCCAGGGTGAAGCAAACGACGGGGATCGTCGGTCTGGATGTCGTCCCCAACGCCCGAGAGGTCTTGATCGGCCTTTACAATAAAACCCTAAAGGAGATCCAGGCCGTTCCCGAGGACGAAGGATACCGTAAGGCGGTCGAGAGCTTCACACGCCACCGCTTGAAGGTCTGCCAGGAGGAAGAGGACTGGGAGCAAATTGAGAAGCGTCTTGGCTGTGGTCAGGTCGAGGAGCTCATCGAGGAGGCTCAGGATGAGCTCAAGCTCATTGGGAAAATGATCGGGAAAAGAACGGAGGTTCACGATTTCTCTAGTACTGAGTGTCAAAGAATGGCATTTGCATCTTGTAGCACTGGAAAAAGCTGGGTTGAGAAGTGGGATCCCTGGGGTGTTCCTGATGACTACGAATGTGAAGTGATCGAGAACGATGCTCCGGTACCCAAGCACATTCCTCTGCATCGACCAGGCCCTCTCCCTGAGGAGTTTTACAAAACGCTGGAGGCCATTAGTGGTGGTAGTGCACAAAAAGTAGAGACGGCCGAGAAAGCATCGGTGACAGAGTGA

Coding sequence (CDS)

ATGTTTCTGCGAGTGATCGGACGGCCATTACTGGCCAGGGTGAAGCAAACGACGGGGATCGTCGGTCTGGATGTCGTCCCCAACGCCCGAGAGGTCTTGATCGGCCTTTACAATAAAACCCTAAAGGAGATCCAGGCCGTTCCCGAGGACGAAGGATACCGTAAGGCGGTCGAGAGCTTCACACGCCACCGCTTGAAGGTCTGCCAGGAGGAAGAGGACTGGGAGCAAATTGAGAAGCGTCTTGGCTGTGGTCAGGTCGAGGAGCTCATCGAGGAGGCTCAGGATGAGCTCAAGCTCATTGGGAAAATGATCGGGAAAAGAACGGAGGTTCACGATTTCTCTAGTACTGAGTGTCAAAGAATGGCATTTGCATCTTGTAGCACTGGAAAAAGCTGGGTTGAGAAGTGGGATCCCTGGGGTGTTCCTGATGACTACGAATGTGAAGTGATCGAGAACGATGCTCCGGTACCCAAGCACATTCCTCTGCATCGACCAGGCCCTCTCCCTGAGGAGTTTTACAAAACGCTGGAGGCCATTAGTGGTGGTAGTGCACAAAAAGTAGAGACGGCCGAGAAAGCATCGGTGACAGAGTGA

Protein sequence

MFLRVIGRPLLARVKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESFTRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLIGKMIGKRTEVHDFSSTECQRMAFASCSTGKSWVEKWDPWGVPDDYECEVIENDAPVPKHIPLHRPGPLPEEFYKTLEAISGGSAQKVETAEKASVTE

Homology

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BLAST of Spg002643 vs. NCBI nr
Match: XP_022945797.1 (probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial [Cucurbita moschata] >XP_023542504.1 probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 302.0 bits (772), Expect = 3.8e-78
Identity = 160/199 (80.40%), Postives = 165/199 (82.91%), Query Frame = 0


Query: 1   MFLRVIGRPLLARVKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESF 60
           MFLRVIGRPLLA+VKQTTGIVGLDVVPNAREVLIGLY KTL+EIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRVIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYGKTLQEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLIGKMIGKRTEVHDFSSTECQR 120
           TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLI KMI                
Sbjct: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLIAKMI---------------- 120

Query: 121 MAFASCSTGKSWVEKWDPWGVPDDYECEVIENDAPVPKHIPLHRPGPLPEEFYKTLEAI- 180
                         +WDPWGVPDDYECEVIENDAPVPKH+PLHRPGPLPEEFYKTLEAI 
Sbjct: 121 --------------EWDPWGVPDDYECEVIENDAPVPKHVPLHRPGPLPEEFYKTLEAIS 169

Query: 181 SGGSAQKVETAEKAS-VTE 198
           SGGSAQKVETA+KAS VTE
Sbjct: 181 SGGSAQKVETADKASQVTE 169

[Close]

BLAST of Spg002643 vs. NCBI nr
Match: XP_004140748.1 (probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial [Cucumis sativus] >KGN57450.2 hypothetical protein Csa_010484 [Cucumis sativus])

HSP 1 Score: 301.2 bits (770), Expect = 6.5e-78
Identity = 158/198 (79.80%), Postives = 162/198 (81.82%), Query Frame = 0


Query: 1   MFLRVIGRPLLARVKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESF 60
           MFLR IGRPLLA+VKQTTGIVGLDVVPNAREVLIGLY+KTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLIGKMIGKRTEVHDFSSTECQR 120
           TRHRLKVCQEEEDWE IEKRLGCGQVEELIEEAQDELKLIGKMI                
Sbjct: 61  TRHRLKVCQEEEDWENIEKRLGCGQVEELIEEAQDELKLIGKMI---------------- 120

Query: 121 MAFASCSTGKSWVEKWDPWGVPDDYECEVIENDAPVPKHIPLHRPGPLPEEFYKTLEAIS 180
                         +WDPWGVPDDYECEVIENDAPVPKHIPLHRPGPLPEEFYKTLEAIS
Sbjct: 121 --------------EWDPWGVPDDYECEVIENDAPVPKHIPLHRPGPLPEEFYKTLEAIS 168

Query: 181 GGSAQKVETAEKAS-VTE 198
           G S +KVET EKAS VTE
Sbjct: 181 GDSTKKVETPEKASQVTE 168

[Close]

BLAST of Spg002643 vs. NCBI nr
Match: KAG6574208.1 (putative NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013272.1 putative NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 300.4 bits (768), Expect = 1.1e-77
Identity = 159/199 (79.90%), Postives = 164/199 (82.41%), Query Frame = 0


Query: 1   MFLRVIGRPLLARVKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESF 60
           MFLRVIGRPLLA+VKQTTGIVGLDVVPNAREVLIGLY KTL+EIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRVIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYGKTLQEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLIGKMIGKRTEVHDFSSTECQR 120
           TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLI KMI                
Sbjct: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLIAKMI---------------- 120

Query: 121 MAFASCSTGKSWVEKWDPWGVPDDYECEVIENDAPVPKHIPLHRPGPLPEEFYKTLEAI- 180
                         +WDPWGVPDDYECEVIENDAPVPKH+PLHRPGPLPEEFYKTLEAI 
Sbjct: 121 --------------EWDPWGVPDDYECEVIENDAPVPKHVPLHRPGPLPEEFYKTLEAIS 169

Query: 181 SGGSAQKVETAEKAS-VTE 198
           SGGSAQKVET +KAS VTE
Sbjct: 181 SGGSAQKVETTDKASQVTE 169

[Close]

BLAST of Spg002643 vs. NCBI nr
Match: XP_022141236.1 (probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial [Momordica charantia])

HSP 1 Score: 300.1 bits (767), Expect = 1.4e-77
Identity = 157/198 (79.29%), Postives = 164/198 (82.83%), Query Frame = 0


Query: 1   MFLRVIGRPLLARVKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESF 60
           MFLRVIGRPLLA+VKQTTGIVGL+VVPNAREVLIGLY+KTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRVIGRPLLAKVKQTTGIVGLEVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLIGKMIGKRTEVHDFSSTECQR 120
           TRHRLKVCQEE+DWE+IEKRLGCGQVEELIEEAQDELKLI KMI                
Sbjct: 61  TRHRLKVCQEEDDWEKIEKRLGCGQVEELIEEAQDELKLIAKMI---------------- 120

Query: 121 MAFASCSTGKSWVEKWDPWGVPDDYECEVIENDAPVPKHIPLHRPGPLPEEFYKTLEAIS 180
                         +WDPWGVPDDYECEVIENDA VPKHIPLHRPGPLPEEFYKTLEAI 
Sbjct: 121 --------------EWDPWGVPDDYECEVIENDASVPKHIPLHRPGPLPEEFYKTLEAIG 168

Query: 181 GGSAQKVETAEKAS-VTE 198
           GGS+QKVETAEKAS VTE
Sbjct: 181 GGSSQKVETAEKASQVTE 168

[Close]

BLAST of Spg002643 vs. NCBI nr
Match: XP_022968495.1 (probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial [Cucurbita maxima])

HSP 1 Score: 299.3 bits (765), Expect = 2.5e-77
Identity = 159/200 (79.50%), Postives = 164/200 (82.00%), Query Frame = 0


Query: 1   MFLRVIGRPLLARVKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESF 60
           MFLRVIGRPLLA+VKQTTGIVGLDVVPNAREVLIGLY KTL+EIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRVIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYGKTLQEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLIGKMIGKRTEVHDFSSTECQR 120
           TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLI KMI                
Sbjct: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLIAKMI---------------- 120

Query: 121 MAFASCSTGKSWVEKWDPWGVPDDYECEVIENDAPVPKHIPLHRPGPLPEEFYKTLEAIS 180
                         +WDPWGVPDDYECEVIENDAPVPKH+PLHRPGPLPEEFYKTLEAIS
Sbjct: 121 --------------EWDPWGVPDDYECEVIENDAPVPKHVPLHRPGPLPEEFYKTLEAIS 170

Query: 181 --GGSAQKVETAEKAS-VTE 198
             G SAQKVETA+KAS VTE
Sbjct: 181 SGGSSAQKVETADKASQVTE 170

[Close]

BLAST of Spg002643 vs. ExPASy Swiss-Prot
Match: Q9FLX7 (Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g52840 PE=1 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 4.4e-69
Identity = 133/190 (70.00%), Postives = 144/190 (75.79%), Query Frame = 0


Query: 1   MFLRVIGRPLLARVKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESF 60
           MFLR IGRPLLA+VKQTTGIVGLDVVPNAR VLI LY+KTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNARAVLIDLYSKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLIGKMIGKRTEVHDFSSTECQR 120
           TR RL VC+EEEDWE IEKRLGCGQVEELIEEA+DEL LIGKMI                
Sbjct: 61  TRQRLNVCKEEEDWEMIEKRLGCGQVEELIEEARDELTLIGKMI---------------- 120

Query: 121 MAFASCSTGKSWVEKWDPWGVPDDYECEVIENDAPVPKHIPLHRPGPLPEEFYKTLEAIS 180
                         +WDPWGVPDDYECEVIENDAP+PKH+P HRPGPLPE+FYKTLE + 
Sbjct: 121 --------------EWDPWGVPDDYECEVIENDAPIPKHVPQHRPGPLPEQFYKTLEGLI 160

Query: 181 GGSAQKVETA 191
             S  ++  A
Sbjct: 181 AESKTEIPAA 160

[Close]

BLAST of Spg002643 vs. ExPASy Swiss-Prot
Match: Q559Z4 (Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Dictyostelium discoideum OX=44689 GN=ndufa5 PE=3 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 5.6e-16
Identity = 41/87 (47.13%), Postives = 63/87 (72.41%), Query Frame = 0


Query: 14  VKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESFTRHRLKVCQEEED 73
           +K++TGI GL V PNAR +L GLYN+ LK+++ +P   GYRK+ E  T+ RL V + E D
Sbjct: 6   IKKSTGITGLKVEPNARHILAGLYNQLLKKLEILPPTAGYRKSAELETKFRLGVIENETD 65

Query: 74  WEQIEKRLGCGQVEELIEEAQDELKLI 101
             +IE ++  GQ+EELI +A+++LK++
Sbjct: 66  IVKIENKIYAGQIEELIVQAKNDLKVV 92

[Close]

BLAST of Spg002643 vs. ExPASy Swiss-Prot
Match: Q18359 (Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Caenorhabditis elegans OX=6239 GN=C33A12.1 PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.4e-11
Identity = 33/84 (39.29%), Postives = 55/84 (65.48%), Query Frame = 0


Query: 15  KQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESFTRHRLKVCQEEEDW 74
           K+TTGI GL V  +    L  +Y + L+ ++ +P D  YRK  E+  + RL + Q E D 
Sbjct: 40  KKTTGITGLFVNEHPHRALTVVYGRILRALEQIPRDAAYRKYTEAVVKQRLALVQAENDI 99

Query: 75  EQIEKRLGCGQVEELIEEAQDELK 99
           +++E+++G GQ+EE+IE+A+ EL+
Sbjct: 100 KKLEEKIGMGQIEEVIEQAEYELE 123

[Close]

BLAST of Spg002643 vs. ExPASy Swiss-Prot
Match: P23935 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Bos taurus OX=9913 GN=NDUFA5 PE=1 SV=3)

HSP 1 Score: 69.7 bits (169), Expect = 4.2e-11
Identity = 38/91 (41.76%), Postives = 57/91 (62.64%), Query Frame = 0


Query: 14  VKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESFTRHRLKVCQEEED 73
           +K+TTG+VGL V     E L  LY K L  +  +P++  YRK  E  T  +L + + E D
Sbjct: 5   LKKTTGLVGLAVCETPHERLKILYTKILDVLGHIPKNAAYRKYTEQITNEKLSMVKAEPD 64

Query: 74  WEQIEKRLGCGQVEELIEEAQDELKLIGKMI 105
            +++E+RL  GQ+EE+I +A++EL L  KMI
Sbjct: 65  VKKLEERLQGGQIEEVILQAENELSLARKMI 95

[Close]

BLAST of Spg002643 vs. ExPASy Swiss-Prot
Match: Q0MQA1 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Gorilla gorilla gorilla OX=9595 GN=NDUFA5 PE=3 SV=3)

HSP 1 Score: 65.1 bits (157), Expect = 1.0e-09
Identity = 36/90 (40.00%), Postives = 55/90 (61.11%), Query Frame = 0


Query: 14  VKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESFTRHRLKVCQEEED 73
           +K+TTG+VGL V     E L  LY K L  ++ +P++  YRK  E  T  +L + + E D
Sbjct: 5   LKKTTGLVGLAVCNTPHERLRILYTKILDVLEEIPKNAAYRKYTEQITNEKLAMVKAEPD 64

Query: 74  WEQIEKRLGCGQVEELIEEAQDELKLIGKM 104
            +++E +L  GQ+EE+I +A+ EL L  KM
Sbjct: 65  VKKLEDQLQGGQLEEVILQAEHELNLARKM 94

[Close]

BLAST of Spg002643 vs. ExPASy TrEMBL
Match: A0A6J1G1X4 (probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111449938 PE=3 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 1.8e-78
Identity = 160/199 (80.40%), Postives = 165/199 (82.91%), Query Frame = 0


Query: 1   MFLRVIGRPLLARVKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESF 60
           MFLRVIGRPLLA+VKQTTGIVGLDVVPNAREVLIGLY KTL+EIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRVIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYGKTLQEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLIGKMIGKRTEVHDFSSTECQR 120
           TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLI KMI                
Sbjct: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLIAKMI---------------- 120

Query: 121 MAFASCSTGKSWVEKWDPWGVPDDYECEVIENDAPVPKHIPLHRPGPLPEEFYKTLEAI- 180
                         +WDPWGVPDDYECEVIENDAPVPKH+PLHRPGPLPEEFYKTLEAI 
Sbjct: 121 --------------EWDPWGVPDDYECEVIENDAPVPKHVPLHRPGPLPEEFYKTLEAIS 169

Query: 181 SGGSAQKVETAEKAS-VTE 198
           SGGSAQKVETA+KAS VTE
Sbjct: 181 SGGSAQKVETADKASQVTE 169

[Close]

BLAST of Spg002643 vs. ExPASy TrEMBL
Match: A0A6J1CII6 (probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111011683 PE=3 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 7.0e-78
Identity = 157/198 (79.29%), Postives = 164/198 (82.83%), Query Frame = 0


Query: 1   MFLRVIGRPLLARVKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESF 60
           MFLRVIGRPLLA+VKQTTGIVGL+VVPNAREVLIGLY+KTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRVIGRPLLAKVKQTTGIVGLEVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLIGKMIGKRTEVHDFSSTECQR 120
           TRHRLKVCQEE+DWE+IEKRLGCGQVEELIEEAQDELKLI KMI                
Sbjct: 61  TRHRLKVCQEEDDWEKIEKRLGCGQVEELIEEAQDELKLIAKMI---------------- 120

Query: 121 MAFASCSTGKSWVEKWDPWGVPDDYECEVIENDAPVPKHIPLHRPGPLPEEFYKTLEAIS 180
                         +WDPWGVPDDYECEVIENDA VPKHIPLHRPGPLPEEFYKTLEAI 
Sbjct: 121 --------------EWDPWGVPDDYECEVIENDASVPKHIPLHRPGPLPEEFYKTLEAIG 168

Query: 181 GGSAQKVETAEKAS-VTE 198
           GGS+QKVETAEKAS VTE
Sbjct: 181 GGSSQKVETAEKASQVTE 168

[Close]

BLAST of Spg002643 vs. ExPASy TrEMBL
Match: A0A6J1HXB9 (probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111467715 PE=3 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 1.2e-77
Identity = 159/200 (79.50%), Postives = 164/200 (82.00%), Query Frame = 0


Query: 1   MFLRVIGRPLLARVKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESF 60
           MFLRVIGRPLLA+VKQTTGIVGLDVVPNAREVLIGLY KTL+EIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRVIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYGKTLQEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLIGKMIGKRTEVHDFSSTECQR 120
           TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLI KMI                
Sbjct: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLIAKMI---------------- 120

Query: 121 MAFASCSTGKSWVEKWDPWGVPDDYECEVIENDAPVPKHIPLHRPGPLPEEFYKTLEAIS 180
                         +WDPWGVPDDYECEVIENDAPVPKH+PLHRPGPLPEEFYKTLEAIS
Sbjct: 121 --------------EWDPWGVPDDYECEVIENDAPVPKHVPLHRPGPLPEEFYKTLEAIS 170

Query: 181 --GGSAQKVETAEKAS-VTE 198
             G SAQKVETA+KAS VTE
Sbjct: 181 SGGSSAQKVETADKASQVTE 170

[Close]

BLAST of Spg002643 vs. ExPASy TrEMBL
Match: A0A1S3AZ47 (probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103484126 PE=3 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 1.6e-77
Identity = 156/198 (78.79%), Postives = 161/198 (81.31%), Query Frame = 0


Query: 1   MFLRVIGRPLLARVKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESF 60
           MFLR IGRPLLA+VKQTTGIVGLDVVPNAREVLIGLY+KTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLIGKMIGKRTEVHDFSSTECQR 120
           TRHRLKVCQEEEDWE IEKRLGCGQVEELIEEAQDELKLI KMI                
Sbjct: 61  TRHRLKVCQEEEDWENIEKRLGCGQVEELIEEAQDELKLIAKMI---------------- 120

Query: 121 MAFASCSTGKSWVEKWDPWGVPDDYECEVIENDAPVPKHIPLHRPGPLPEEFYKTLEAIS 180
                         +WDPWGVPDDYECEVIENDAPVPKH+PLHRPGPLPEEFYKTLEAIS
Sbjct: 121 --------------EWDPWGVPDDYECEVIENDAPVPKHVPLHRPGPLPEEFYKTLEAIS 168

Query: 181 GGSAQKVETAEKAS-VTE 198
           G S +KVET EKAS VTE
Sbjct: 181 GDSTRKVETTEKASQVTE 168

[Close]

BLAST of Spg002643 vs. ExPASy TrEMBL
Match: A0A5D3DH67 (Putative NADH dehydrogenase (Ubiquinone) 1 alpha subcomplex subunit 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G001080 PE=3 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 2.0e-77
Identity = 153/194 (78.87%), Postives = 158/194 (81.44%), Query Frame = 0


Query: 1   MFLRVIGRPLLARVKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESF 60
           MFLR IGRPLLA+VKQTTGIVGLDVVPNAREVLIGLY+KTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNAREVLIGLYSKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLIGKMIGKRTEVHDFSSTECQR 120
           TRHRLKVCQEEEDWE IEKRLGCGQVEELIEEAQDELKLI KMI                
Sbjct: 61  TRHRLKVCQEEEDWENIEKRLGCGQVEELIEEAQDELKLIAKMI---------------- 120

Query: 121 MAFASCSTGKSWVEKWDPWGVPDDYECEVIENDAPVPKHIPLHRPGPLPEEFYKTLEAIS 180
                         +WDPWGVPDDYECEVIENDAPVPKH+PLHRPGPLPEEFYKTLEAIS
Sbjct: 121 --------------EWDPWGVPDDYECEVIENDAPVPKHVPLHRPGPLPEEFYKTLEAIS 164

Query: 181 GGSAQKVETAEKAS 195
           G S +KVET EKAS
Sbjct: 181 GDSTRKVETTEKAS 164

[Close]

BLAST of Spg002643 vs. TAIR 10
Match: AT5G52840.1 (NADH-ubiquinone oxidoreductase-related )

HSP 1 Score: 262.3 bits (669), Expect = 3.1e-70
Identity = 133/190 (70.00%), Postives = 144/190 (75.79%), Query Frame = 0


Query: 1   MFLRVIGRPLLARVKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESF 60
           MFLR IGRPLLA+VKQTTGIVGLDVVPNAR VLI LY+KTLKEIQAVPEDEGYRKAVESF
Sbjct: 1   MFLRAIGRPLLAKVKQTTGIVGLDVVPNARAVLIDLYSKTLKEIQAVPEDEGYRKAVESF 60

Query: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLIGKMIGKRTEVHDFSSTECQR 120
           TR RL VC+EEEDWE IEKRLGCGQVEELIEEA+DEL LIGKMI                
Sbjct: 61  TRQRLNVCKEEEDWEMIEKRLGCGQVEELIEEARDELTLIGKMI---------------- 120

Query: 121 MAFASCSTGKSWVEKWDPWGVPDDYECEVIENDAPVPKHIPLHRPGPLPEEFYKTLEAIS 180
                         +WDPWGVPDDYECEVIENDAP+PKH+P HRPGPLPE+FYKTLE + 
Sbjct: 121 --------------EWDPWGVPDDYECEVIENDAPIPKHVPQHRPGPLPEQFYKTLEGLI 160

Query: 181 GGSAQKVETA 191
             S  ++  A
Sbjct: 181 AESKTEIPAA 160

[Close]

BLAST of Spg002643 vs. TAIR 10
Match: AT4G28005.1 (BEST Arabidopsis thaliana protein match is: NADH-ubiquinone oxidoreductase-related (TAIR:AT5G52840.1); Has 37 Blast hits to 37 proteins in 15 species: Archae - 0; Bacteria - 0; Metazoa - 2; Fungi - 0; Plants - 33; Viruses - 0; Other Eukaryotes - 2 (source: NCBI BLink). )

HSP 1 Score: 104.8 bits (260), Expect = 8.3e-23
Identity = 75/194 (38.66%), Postives = 89/194 (45.88%), Query Frame = 0


Query: 1   MFLRVIGRPLLARVKQTTGIVGLDVVPNAREVLIGLYNKTLKEIQAVPEDEGYRKAVESF 60
           MF RVIGRPLLA+VKQTTGIV                                       
Sbjct: 1   MFQRVIGRPLLAKVKQTTGIV--------------------------------------- 60

Query: 61  TRHRLKVCQEEEDWEQIEKRLGCGQVEELIEEAQDELKLIGKMIGKRTEVHDFSSTECQR 120
                +VC EEEDW  IEKRL CG+VEELIEEA DEL L+GKMIG    +H         
Sbjct: 61  -----EVCPEEEDWGVIEKRLACGKVEELIEEASDELTLVGKMIG---SLHPNLIL---- 109

Query: 121 MAFASCSTGKSWVEKWDPWGVPDDYECEVIENDAPVPKHIPLHRPGPLPEEFYKTLEAIS 180
                                            AP+PKH+P HRPGPLPEEFY+TL+A++
Sbjct: 121 ---------------------------------APIPKHVPHHRPGPLPEEFYRTLQAVN 109

Query: 181 GGSAQKVETAEKAS 195
             S  ++ TA  +S
Sbjct: 181 SESKTEI-TATSSS 109

The following BLAST results are available for this feature:

BLAST of Spg002643 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022945797.1	3.8e-78	80.40	probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochond...	[more]
XP_004140748.1	6.5e-78	79.80	probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochond...	[more]
KAG6574208.1	1.1e-77	79.90	putative NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochond...	[more]
XP_022141236.1	1.4e-77	79.29	probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochond...	[more]
XP_022968495.1	2.5e-77	79.50	probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochond...	[more]

BLAST of Spg002643 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FLX7	4.4e-69	70.00	Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochond...	[more]
Q559Z4	5.6e-16	47.13	Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Dictyos...	[more]
Q18359	2.4e-11	39.29	Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Caenorh...	[more]
P23935	4.2e-11	41.76	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Bos taurus OX=99...	[more]
Q0MQA1	1.0e-09	40.00	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 OS=Gorilla gorilla ...	[more]

BLAST of Spg002643 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G1X4	1.8e-78	80.40	probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochond...	[more]
A0A6J1CII6	7.0e-78	79.29	probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochond...	[more]
A0A6J1HXB9	1.2e-77	79.50	probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochond...	[more]
A0A1S3AZ47	1.6e-77	78.79	probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochond...	[more]
A0A5D3DH67	2.0e-77	78.87	Putative NADH dehydrogenase (Ubiquinone) 1 alpha subcomplex subunit 5 OS=Cucumis...	[more]

BLAST of Spg002643 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G52840.1	3.1e-70	70.00	NADH-ubiquinone oxidoreductase-related	[more]
AT4G28005.1	8.3e-23	38.66	BEST Arabidopsis thaliana protein match is: NADH-ubiquinone oxidoreductase-relat...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006806	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5	PFAM	PF04716	ETC_C1_NDUFA5	coord: 22..88 e-value: 7.6E-28 score: 96.1
IPR006806	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5	PANTHER	PTHR12653	NADH-UBIQUINONE OXIDOREDUCTASE 13 KD-B SUBUNIT	coord: 1..105
IPR006806	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5	PANTHER	PTHR12653	NADH-UBIQUINONE OXIDOREDUCTASE 13 KD-B SUBUNIT	coord: 135..193
None	No IPR available	PANTHER	PTHR12653:SF1	BNAA02G10640D PROTEIN	coord: 135..193
None	No IPR available	PANTHER	PTHR12653:SF1	BNAA02G10640D PROTEIN	coord: 1..105

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg002643.1	Spg002643.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022904	respiratory electron transport chain
cellular_component	GO:0005743	mitochondrial inner membrane
cellular_component	GO:0045271	respiratory chain complex I