Spg002461 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg002461
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionspeckle-type POZ protein-like A
Locationscaffold6: 2924452 .. 2926748 (+)
RNA-Seq ExpressionSpg002461
SyntenySpg002461
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AACATTGAGACATAATGATTATGGATGATGATAACATGTTGTACGAGGACGGTCCTGTAAAAGATGAACCACAACATGGTAAATAGTCTTTATAATTTTTATAAAACGTTTTATTATATTCTATGTCATATTGATTCCTCAATCTTCCATATACTCATTTTCTTCATTTTTTCGTCAAACACGATGTGAAAATTGTAGAGCAATATTGAAAGTGCACGAATCAAAATGAAATGAACATATACAAAGTTCAAGAACCCAAATGAGATTTGTCTGATATTTGTTATTTATTCGATAAACGCATGTTCGGGTTACGATTTTGTGTTACTAATGTTCACATTTTTTTGCATGCTTTTTTAATACAGAAATTACAAGATCAAGAAGGGTTGATCCACCAGCTGATTATACATTCCGAATTAATTCTTACTCTGTACTTTCAGAAATAGGGATGAAGAAATGTGAATCTGGAGACTTTGAAATTGATGGCTACAAGTGGTTTGGCAGCTAAACTTAATTTTAAATTGAAGTATCATAAACTAGGGTTAGGGTTTATAATTAATTGATTAATTAATTTGTTAATCTCTATTATATATTTCTCACTTAGGAAATTGATTCTATATCCAAATGGAGATGATAAAGTGGATGATCATATTTCTCTCTACTTGGCTATATCATCTAAAGATAATCTTCCTCTTGGCTGGGAAGTGAAGGTTATTTTCAGGTTTTTTATATTTGATCAAATCAAAGACAACTATTTAACAATACAAGGTAAATATATTCATTACTCTTTATTTCCTAATTTCTGTTTTTTTTTTAGGTTAAATTATAAAAAATGTCCCTGAACTATGGGGTTGGTTTCAATTATACTCATGAACTTTCAAAAGCTTCATTTTAGTCCTTGAAATTTGAAGTTTGTTTCAAACTAGTCCTTGAAGACTTTTTTCGTTAAATTCATGAACGAAAAATGACGTGTTTGCATATGTATCGTTGCTTTTTGTGAATAGATTTGAATATATACGTTAAAAAAAAACATATGCAAACACATCATTTTTTGTCAATGAATTTAATAGAAAATGTTTGCAAGGGTCATTTTGAAACAAACTTCAAACTTCAAGGTTTGAAAGTTCATGGATATAACTGTACCTAATCTTATATAGTTAAGAGGTATTTTTGATAATCTAACCTTTTTTAAATCAAATAACATTTCCCATAACCAAGTTTATAAAAAAATATTGCTACGAATGACGTCGAAACTCGAAAAAAATAGTTATCAAATAGGAATAAAGTTTGGTTTTGCAGATGGGAAAGTGAGGAAGTATTCTAAAATGAAGAGTGAACATGGATTCAGCCAGTTGGTTTCTCATAAAGCTTTCAATAAAGCCTCCAATGGCTTGCTTGTGGATAATTGCTGCACATTTGGAGTTGAAGTTTCTCTACTTAAACCCTCAAACAAAGGAGAGAGTTTAACCATTATTAAAGAGCCTCAGCAGTTTGAATCCTCTTATTGGAGCATCTATTCATTTTCTACTAAAACTGATGAATCCTATGTCTCAAATCCTTTCAATGTCAAAGGAAGACAATGGTTAGTAATATACCATATTTTTCTTTTTCAAAAAATAGAAAATAAAAAACAAAATCGTTATCAAATAGGTCTGCTTACGACTCGTTTGGCTTATTTTTACTTCAGGAGAATGAAAATATACCCGAACGGTTTTTCTTCGGGAAAGACCACACATCTCTCTCTTTACCTTAAGTTGGATAGTTCAGAGACCATTCCAGTGGGAAAGAAAATATATGCAAAATTCATTCTGAGTGTTTATAACAGGGTAGACAGGAAATATATTGACAAATCAGGTAAGTTTTTATTTGATAATTCTCTTGATTTTTTCAAAGTATTTTTCCATTTATTTTTAGAAAAGAAAGTATTTTCTAAATAATAGACCTTTTTTTTTTTTTTTTTGGGAATCTTATGTGGCATTGCATTTTGCAGCTCGAGAGCGTTGGTTTGATAACACAACCTATGGCAGTGGCTTTCCTGAGTTCTTGTCTAGAAAAGACCTCAGCAATCATTTGGATGGTGATGTTTTATATCTAAAAGCTAGAATTGTTGCTATGTCAACTGTGGAAGAGTTTTAAAAAATGATCCCTCAACCATACGTCGAGATGTTGCATTAACATATGATAGTAAATGTGTGTATATATCTATACATGATATAGAATGTATTCGTGTTTAAAAGTCAATCAATATAATGGACTTTCAACTCGGAATTATTTTTCACTTATCTTGAGTTGAGATAGCA

mRNA sequence

ATGATTATGGATGATGATAACATGTTGTACGAGGACGGTCCTGTAAAAGATGAACCACAACATGAAATTACAAGATCAAGAAGGGTTGATCCACCAGCTGATTATACATTCCGAATTAATTCTTACTCTGTACTTTCAGAAATAGGGATGAAGAAATGTGAATCTGGAGACTTTGAAATTGATGGCTACAAGTGGAAATTGATTCTATATCCAAATGGAGATGATAAAGTGGATGATCATATTTCTCTCTACTTGGCTATATCATCTAAAGATAATCTTCCTCTTGGCTGGGAAGTGAAGGTTATTTTCAGGTTTTTTATATTTGATCAAATCAAAGACAACTATTTAACAATACAAGATGGGAAAGTGAGGAAGTATTCTAAAATGAAGAGTGAACATGGATTCAGCCAGTTGGTTTCTCATAAAGCTTTCAATAAAGCCTCCAATGGCTTGCTTGTGGATAATTGCTGCACATTTGGAGTTGAAGTTTCTCTACTTAAACCCTCAAACAAAGGAGAGAGTTTAACCATTATTAAAGAGCCTCAGCAGTTTGAATCCTCTTATTGGAGCATCTATTCATTTTCTACTAAAACTGATGAATCCTATGTCTCAAATCCTTTCAATGTCAAAGGAAGACAATGGAGAATGAAAATATACCCGAACGGTTTTTCTTCGGGAAAGACCACACATCTCTCTCTTTACCTTAAGTTGGATAGTTCAGAGACCATTCCAGTGGGAAAGAAAATATATGCAAAATTCATTCTGAGTGTTTATAACAGGGTAGACAGGAAATATATTGACAAATCAGCTCGAGAGCGTTGGTTTGATAACACAACCTATGGCAGTGGCTTTCCTGAGTTCTTGTCTAGAAAAGACCTCAGCAATCATTTGGATGGTGATGTTTTATATCTAAAAGCTAGAATTGTTGCTATGTCAACTGTGGAAGAGTTTTAA

Coding sequence (CDS)

ATGATTATGGATGATGATAACATGTTGTACGAGGACGGTCCTGTAAAAGATGAACCACAACATGAAATTACAAGATCAAGAAGGGTTGATCCACCAGCTGATTATACATTCCGAATTAATTCTTACTCTGTACTTTCAGAAATAGGGATGAAGAAATGTGAATCTGGAGACTTTGAAATTGATGGCTACAAGTGGAAATTGATTCTATATCCAAATGGAGATGATAAAGTGGATGATCATATTTCTCTCTACTTGGCTATATCATCTAAAGATAATCTTCCTCTTGGCTGGGAAGTGAAGGTTATTTTCAGGTTTTTTATATTTGATCAAATCAAAGACAACTATTTAACAATACAAGATGGGAAAGTGAGGAAGTATTCTAAAATGAAGAGTGAACATGGATTCAGCCAGTTGGTTTCTCATAAAGCTTTCAATAAAGCCTCCAATGGCTTGCTTGTGGATAATTGCTGCACATTTGGAGTTGAAGTTTCTCTACTTAAACCCTCAAACAAAGGAGAGAGTTTAACCATTATTAAAGAGCCTCAGCAGTTTGAATCCTCTTATTGGAGCATCTATTCATTTTCTACTAAAACTGATGAATCCTATGTCTCAAATCCTTTCAATGTCAAAGGAAGACAATGGAGAATGAAAATATACCCGAACGGTTTTTCTTCGGGAAAGACCACACATCTCTCTCTTTACCTTAAGTTGGATAGTTCAGAGACCATTCCAGTGGGAAAGAAAATATATGCAAAATTCATTCTGAGTGTTTATAACAGGGTAGACAGGAAATATATTGACAAATCAGCTCGAGAGCGTTGGTTTGATAACACAACCTATGGCAGTGGCTTTCCTGAGTTCTTGTCTAGAAAAGACCTCAGCAATCATTTGGATGGTGATGTTTTATATCTAAAAGCTAGAATTGTTGCTATGTCAACTGTGGAAGAGTTTTAA

Protein sequence

MIMDDDNMLYEDGPVKDEPQHEITRSRRVDPPADYTFRINSYSVLSEIGMKKCESGDFEIDGYKWKLILYPNGDDKVDDHISLYLAISSKDNLPLGWEVKVIFRFFIFDQIKDNYLTIQDGKVRKYSKMKSEHGFSQLVSHKAFNKASNGLLVDNCCTFGVEVSLLKPSNKGESLTIIKEPQQFESSYWSIYSFSTKTDESYVSNPFNVKGRQWRMKIYPNGFSSGKTTHLSLYLKLDSSETIPVGKKIYAKFILSVYNRVDRKYIDKSARERWFDNTTYGSGFPEFLSRKDLSNHLDGDVLYLKARIVAMSTVEEF
Homology
BLAST of Spg002461 vs. NCBI nr
Match: XP_038902857.1 (MATH domain and coiled-coil domain-containing protein At3g58400-like [Benincasa hispida])

HSP 1 Score: 492.7 bits (1267), Expect = 2.4e-135
Identity = 242/314 (77.07%), Postives = 274/314 (87.26%), Query Frame = 0

Query: 5   DDNMLYEDGPVKDEPQHE-ITRSRRVDPPADYTFRINSYSVLSEIGMKKCESGDFEIDGY 64
           DDNM Y+D  + DE QHE ITRSRRVDPP+ Y F+I+SYSV SEI MKKCESGDFE+DGY
Sbjct: 2   DDNMSYDD-TINDEQQHEIITRSRRVDPPSHYAFKIDSYSVFSEIEMKKCESGDFEVDGY 61

Query: 65  KWKLILYPNGDDKVDDHISLYLAISSKDNLPLGWEVKVIFRFFIFDQIKDNYLTIQDGKV 124
           KWKLILYPNGD++V DHISLYLAIS+KDNLPLGWEV+VIFRFFIFDQI+DNYLTIQDGK+
Sbjct: 62  KWKLILYPNGDEEVQDHISLYLAISAKDNLPLGWEVRVIFRFFIFDQIRDNYLTIQDGKM 121

Query: 125 RKYSKMKSEHGFSQLVSHKAFNKASNGLLVDNCCTFGVEVSLLKPSNKGESLTIIKEPQQ 184
           R+YSKMKSEHGF+QL+SH  FNKASNG LVDN CTFGVE+S+LK SNKGE LTI KEPQQ
Sbjct: 122 RRYSKMKSEHGFTQLLSHNIFNKASNGFLVDNSCTFGVEISVLKASNKGEILTIYKEPQQ 181

Query: 185 FESSYWSIYSFSTKTDESYVSNPFNVKGRQWRMKIYPNGFSSGKTTHLSLYLKLDSSETI 244
            ++ +WSIYSFS K +E Y+S PFNVKGRQWRM IYPNG S GKT+H+SLYL+LDSSETI
Sbjct: 182 -DTFFWSIYSFSVKINEFYISEPFNVKGRQWRMVIYPNGHSRGKTSHISLYLRLDSSETI 241

Query: 245 PVGKKIYAKFILSVYNRVDRKYIDKSARERWFDNTTYGSGFPEFLSRKDLSNHLDGDVLY 304
           P+GKKIYAKF+L VYN V +KYIDKS +E WF  T+ G+GF EFLSRKD+S HL  DVLY
Sbjct: 242 PLGKKIYAKFLLGVYNFVAKKYIDKS-KEVWFAATSTGNGFHEFLSRKDMSPHLQNDVLY 301

Query: 305 LKARIVAMSTVEEF 318
           LKARIVAMS VEEF
Sbjct: 302 LKARIVAMSAVEEF 312

BLAST of Spg002461 vs. NCBI nr
Match: KAG6600198.1 (Ubiquitin carboxyl-terminal hydrolase 12, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 482.3 bits (1240), Expect = 3.3e-132
Identity = 234/314 (74.52%), Postives = 270/314 (85.99%), Query Frame = 0

Query: 5   DDNMLYEDGPVKDEPQHEITRSRRVDPPADYTFRINSYSVLSEIGMKKCESGDFEIDGYK 64
           DDNM Y+D P+K E Q EITR+RRV+PPA+YTF+INSYS LSEIGMKKCESG+F +DG+ 
Sbjct: 89  DDNMSYDD-PIKHEQQPEITRTRRVEPPANYTFQINSYSTLSEIGMKKCESGNFAVDGFN 148

Query: 65  WKLILYPNGDDKVDDHISLYLAISSKDNLPLGWEVKVIFRFFIFDQIKDNYLTIQDGKVR 124
           WKLILYPNGDD+V+DHISLYLAI+SKDNLPLGWEVKVIFRFF++D+IKDNYLT+QDGK+R
Sbjct: 149 WKLILYPNGDDEVEDHISLYLAITSKDNLPLGWEVKVIFRFFVYDRIKDNYLTVQDGKMR 208

Query: 125 KYSKMKSEHGFSQLVSHKAFNKASNGLLVDNCCTFGVEVSLLKPSNKGESLTIIKEPQQF 184
           KYSKMKSEHGF++ ++H AFN+ASNG LVDN CTFGVEVS+LK SNKGESLTI KEP Q 
Sbjct: 209 KYSKMKSEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEP-QL 268

Query: 185 ESSYWSIYSFSTKTDESYVSNPFNVKGRQWRMKIYPNGFSSGKTTHLSLYLKLDSSETIP 244
            S +WSI SFS KT+E Y+S PF VKGR+WR+ IYPNG SSGK +H+SLYLKLDSSET+P
Sbjct: 269 GSFFWSISSFSNKTNEFYLSEPFTVKGRKWRLTIYPNGLSSGKMSHISLYLKLDSSETVP 328

Query: 245 VGKKIYAKFILSVYNRVDRKYIDKSARERWFDNTTYGSGFPEFLSRKDLS-NHLDGDVLY 304
            GKKIYAKF LSVYN   +KY+D+S  E WFDNTTYG GF EFLSR DL  +HL  DV Y
Sbjct: 329 TGKKIYAKFFLSVYNFGAKKYVDQS-NEHWFDNTTYGYGFHEFLSRNDLQPHHLQNDVFY 388

Query: 305 LKARIVAMSTVEEF 318
           LKARI AMS VE+F
Sbjct: 389 LKARIAAMSVVEDF 399

BLAST of Spg002461 vs. NCBI nr
Match: XP_023529004.1 (speckle-type POZ protein-like A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 481.9 bits (1239), Expect = 4.3e-132
Identity = 235/313 (75.08%), Postives = 269/313 (85.94%), Query Frame = 0

Query: 6   DNMLYEDGPVKDEPQHEITRSRRVDPPADYTFRINSYSVLSEIGMKKCESGDFEIDGYKW 65
           DNM Y+D P+K E Q EITR+RRV+PPA+YTF+INSYS LSEIGMKKCESG+F +DGY W
Sbjct: 3   DNMSYDD-PIKREQQPEITRTRRVEPPANYTFQINSYSTLSEIGMKKCESGNFAVDGYNW 62

Query: 66  KLILYPNGDDKVDDHISLYLAISSKDNLPLGWEVKVIFRFFIFDQIKDNYLTIQDGKVRK 125
           KLILYPNGDD+V+DHISLYLAI SKDNLPLGWEVKVIFRFF++D+IKDNYLT+QDGK+RK
Sbjct: 63  KLILYPNGDDEVEDHISLYLAIISKDNLPLGWEVKVIFRFFVYDRIKDNYLTVQDGKMRK 122

Query: 126 YSKMKSEHGFSQLVSHKAFNKASNGLLVDNCCTFGVEVSLLKPSNKGESLTIIKEPQQFE 185
           YSKMKSEHGF++ ++H AFN+ASNG LVDN CTFGVEVS+LK SNKGESLTI KEP Q  
Sbjct: 123 YSKMKSEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEP-QLG 182

Query: 186 SSYWSIYSFSTKTDESYVSNPFNVKGRQWRMKIYPNGFSSGKTTHLSLYLKLDSSETIPV 245
           S +WSI SFS KT+E Y+S PF VKGR+WR+ IYPNG SSGKT+H+SLYLKLDSSET+P 
Sbjct: 183 SFFWSISSFSNKTNEFYLSEPFTVKGRKWRLMIYPNGLSSGKTSHISLYLKLDSSETVPT 242

Query: 246 GKKIYAKFILSVYNRVDRKYIDKSARERWFDNTTYGSGFPEFLSRKDLS-NHLDGDVLYL 305
           GKKIYAKF LSVYN   +KY+D+S  E WFDNT YG GF EFLSR DL  +HL  DV YL
Sbjct: 243 GKKIYAKFFLSVYNFGAKKYVDQS-NEYWFDNTAYGHGFHEFLSRNDLQPHHLQNDVFYL 302

Query: 306 KARIVAMSTVEEF 318
           KARIVAMS VE+F
Sbjct: 303 KARIVAMSVVEDF 312

BLAST of Spg002461 vs. NCBI nr
Match: XP_022989347.1 (uncharacterized protein LOC111486429 [Cucurbita maxima])

HSP 1 Score: 476.9 bits (1226), Expect = 1.4e-130
Identity = 234/313 (74.76%), Postives = 265/313 (84.66%), Query Frame = 0

Query: 6   DNMLYEDGPVKDEPQHEITRSRRVDPPADYTFRINSYSVLSEIGMKKCESGDFEIDGYKW 65
           DNM Y+D P+K E Q EITR+ RVDPPA+Y F+INSYS LSEIGMKKCESG F +DGY W
Sbjct: 3   DNMSYDD-PIKHEQQPEITRTTRVDPPANYIFQINSYSTLSEIGMKKCESGHFAVDGYNW 62

Query: 66  KLILYPNGDDKVDDHISLYLAISSKDNLPLGWEVKVIFRFFIFDQIKDNYLTIQDGKVRK 125
           KLIL+PNGDDKV+DHISLYLAI+SKDNLPLGWEVKVIFRFF++DQIKDNYLTIQDGK+RK
Sbjct: 63  KLILFPNGDDKVEDHISLYLAITSKDNLPLGWEVKVIFRFFVYDQIKDNYLTIQDGKMRK 122

Query: 126 YSKMKSEHGFSQLVSHKAFNKASNGLLVDNCCTFGVEVSLLKPSNKGESLTIIKEPQQFE 185
           YSKMK+EHGF++ ++H AFN+ASNG LVDN CTFGVEVS+LK SNKGESLTI KEP Q  
Sbjct: 123 YSKMKTEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEP-QLG 182

Query: 186 SSYWSIYSFSTKTDESYVSNPFNVKGRQWRMKIYPNGFSSGKTTHLSLYLKLDSSETIPV 245
           S +WSI SFS K +E Y S PF VKGR+WR+ IYPNGF SGKT+H+SLYLKLDSSETIP 
Sbjct: 183 SFFWSISSFSNKNNEFYFSEPFTVKGRKWRLMIYPNGFLSGKTSHISLYLKLDSSETIPT 242

Query: 246 GKKIYAKFILSVYNRVDRKYIDKSARERWFDNTTYGSGFPEFLSRKDLS-NHLDGDVLYL 305
           GKKIYAKF LSVYN   +KY+D+S  E WFDNT YG GF EFLSR DL  +HL  DV YL
Sbjct: 243 GKKIYAKFFLSVYNFGAKKYVDQS-NEYWFDNTAYGHGFHEFLSRNDLQPHHLQNDVFYL 302

Query: 306 KARIVAMSTVEEF 318
           KARIVAMS V++F
Sbjct: 303 KARIVAMSVVQDF 312

BLAST of Spg002461 vs. NCBI nr
Match: XP_022941740.1 (speckle-type POZ protein-like A [Cucurbita moschata])

HSP 1 Score: 472.6 bits (1215), Expect = 2.6e-129
Identity = 229/313 (73.16%), Postives = 267/313 (85.30%), Query Frame = 0

Query: 6   DNMLYEDGPVKDEPQHEITRSRRVDPPADYTFRINSYSVLSEIGMKKCESGDFEIDGYKW 65
           DNM Y+D P+K E Q EITR+RR +PPA+YTF+INSYS LSEIGMKKCESG+F +DG+ W
Sbjct: 3   DNMSYDD-PIKHEQQPEITRTRRAEPPANYTFQINSYSTLSEIGMKKCESGNFAVDGFNW 62

Query: 66  KLILYPNGDDKVDDHISLYLAISSKDNLPLGWEVKVIFRFFIFDQIKDNYLTIQDGKVRK 125
           KLILYPNGDD+V+DHISLYLAI+SKDNLPLGWEVKVIFRFF++D+IKDNYLT+QDGK+RK
Sbjct: 63  KLILYPNGDDEVEDHISLYLAITSKDNLPLGWEVKVIFRFFVYDRIKDNYLTVQDGKMRK 122

Query: 126 YSKMKSEHGFSQLVSHKAFNKASNGLLVDNCCTFGVEVSLLKPSNKGESLTIIKEPQQFE 185
           YSKMKSEHGF++ ++H AFN+ASNG LVDN CTFGVEVS+LK SNKGESLTI KEP Q  
Sbjct: 123 YSKMKSEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEP-QLG 182

Query: 186 SSYWSIYSFSTKTDESYVSNPFNVKGRQWRMKIYPNGFSSGKTTHLSLYLKLDSSETIPV 245
           S +WSI SFS KT+E Y+S PF VKGR+WR+ IYPNG SSGK +H+SLYLKLDSSET+P 
Sbjct: 183 SFFWSISSFSNKTNEFYLSEPFTVKGRKWRLMIYPNGLSSGKMSHISLYLKLDSSETVPT 242

Query: 246 GKKIYAKFILSVYNRVDRKYIDKSARERWFDNTTYGSGFPEFLSRKDL-SNHLDGDVLYL 305
           GKKIYAKF LSVY+   ++Y+D+S  E WFDNTTYG GF EFLSR DL  +HL  D  YL
Sbjct: 243 GKKIYAKFFLSVYDFGAKRYVDQS-NEHWFDNTTYGYGFHEFLSRNDLRPHHLQNDGFYL 302

Query: 306 KARIVAMSTVEEF 318
           KARI AMS VE+F
Sbjct: 303 KARIAAMSVVEDF 312

BLAST of Spg002461 vs. ExPASy Swiss-Prot
Match: Q9M2J1 (MATH domain and coiled-coil domain-containing protein At3g58250 OS=Arabidopsis thaliana OX=3702 GN=At3g58250 PE=4 SV=2)

HSP 1 Score: 70.5 bits (171), Expect = 3.9e-11
Identity = 37/122 (30.33%), Postives = 62/122 (50.82%), Query Frame = 0

Query: 46  SEIGMKKCESGDFEIDGYKWKLILYPNGDDKVDDHISLYLAISSKDNLPLGWEVKVIFRF 105
           S +  +K  S  F IDG +W+L+ +P G+D   DH+SLYL ++  ++LP GW     F F
Sbjct: 19  SSLQSEKIYSDQFVIDGCRWRLLAFPKGNDTKSDHLSLYLDVAESESLPCGWRRHAQFSF 78

Query: 106 FIFDQIKDNYLTIQDGKVRKYSKMKSEHGFSQLVSHKAFNKASNGLLVDNCCTFGVEVSL 165
            I + I +   + +   +  + +  S+ GF+ LV         +G LV       VE+ +
Sbjct: 79  TIVNHIPEK-CSQRKETIHWFCEKVSDWGFTNLVPLIELKAEDSGFLVKGELKIVVEIEV 138

Query: 166 LK 168
           L+
Sbjct: 139 LE 139

BLAST of Spg002461 vs. ExPASy Swiss-Prot
Match: Q9M2H9 (MATH domain and coiled-coil domain-containing protein At3g58370 OS=Arabidopsis thaliana OX=3702 GN=At3g58370 PE=4 SV=3)

HSP 1 Score: 70.1 bits (170), Expect = 5.1e-11
Identity = 38/131 (29.01%), Postives = 69/131 (52.67%), Query Frame = 0

Query: 180 EPQQFESSY-WSIYSFSTKTDESYVSNPFNVKGRQWRMKIYPNGFSSGKTTHLSLYLKLD 239
           E ++ ++ + W I +FST   +   S+PF + G +W +  YP G     +  LSLYL +D
Sbjct: 2   ESEEVDNKFTWVIKNFSTLQYDKIYSDPFVIGGCKWHLLAYPKGNKFNNS--LSLYLVVD 61

Query: 240 SSETIPVGKKIYAKFILSVYNRVDRKYIDKSARERWFDNTTYGSGFPEFLSRKDLSNHLD 299
            +  +P G + YA+F L++ N++  K   +  ++ WF+    GSGF   +   +L     
Sbjct: 62  DARALPCGWRRYAQFSLTIINQLTDKLSQRGEKQNWFNQRNLGSGFTSMIPLPNLHAKNA 121

Query: 300 GDVLYLKARIV 310
           G ++  + +IV
Sbjct: 122 GYLVNGEVKIV 130

BLAST of Spg002461 vs. ExPASy Swiss-Prot
Match: Q84WU2 (Ubiquitin C-terminal hydrolase 13 OS=Arabidopsis thaliana OX=3702 GN=UBP13 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.1e-10
Identity = 37/139 (26.62%), Postives = 75/139 (53.96%), Query Frame = 0

Query: 30  DPPA-DYTFRINSYSVLSEIGMKKCESGDFEIDGYKWKLILYPNGDDKVDDHISLYLAIS 89
           DPP+  +T+ I    + + +  +K  S  F + GYKW+++++P G++   DH+S+YL ++
Sbjct: 50  DPPSLKFTWTI---PMFTRLNTRKHYSDVFVVGGYKWRILIFPKGNNV--DHLSMYLDVA 109

Query: 90  SKDNLPLGWEVKVIFRFFIFDQIKDNYLTIQDGKVRKYSKMKSEHGFSQLVSHKAFNKAS 149
              NLP GW     F   + +Q+ + Y +I+     +++  +S+ GF+  +      + +
Sbjct: 110 DAANLPYGWSRYSQFSLAVVNQVNNRY-SIRKETQHQFNARESDWGFTSFMPLSELYEPT 169

Query: 150 NGLLVDNCCTFGVEVSLLK 168
            G LV++      EV++ K
Sbjct: 170 RGYLVNDTVLIEAEVAVRK 182

BLAST of Spg002461 vs. ExPASy Swiss-Prot
Match: Q9FPT1 (Ubiquitin C-terminal hydrolase 12 OS=Arabidopsis thaliana OX=3702 GN=UBP12 PE=1 SV=2)

HSP 1 Score: 68.6 bits (166), Expect = 1.5e-10
Identity = 47/177 (26.55%), Postives = 85/177 (48.02%), Query Frame = 0

Query: 4   DDDNMLYEDGPVKDEPQH--EITRSRRV----------DPPA-DYTFRINSYSVLSEIGM 63
           +D+ ML  +  + D P    E+T+              DPP   +T+ I ++   S    
Sbjct: 13  EDEEMLVPNSDLVDGPAQPMEVTQPETAASTVENQPAEDPPTLKFTWTIPNF---SRQNT 72

Query: 64  KKCESGDFEIDGYKWKLILYPNGDDKVDDHISLYLAISSKDNLPLGWEVKVIFRFFIFDQ 123
           +K  S  F + GYKW+++++P G++   DH+S+YL +S   +LP GW     F   + +Q
Sbjct: 73  RKHYSDVFVVGGYKWRILIFPKGNNV--DHLSMYLDVSDAASLPYGWSRYAQFSLAVVNQ 132

Query: 124 IKDNYLTIQDGKVRKYSKMKSEHGFSQLVSHKAFNKASNGLLVDNCCTFGVEVSLLK 168
           I   Y T++     +++  +S+ GF+  +        S G LV++      EV++ K
Sbjct: 133 IHTRY-TVRKETQHQFNARESDWGFTSFMPLSELYDPSRGYLVNDTVLVEAEVAVRK 183

BLAST of Spg002461 vs. ExPASy Swiss-Prot
Match: Q9M2I9 (MATH domain and coiled-coil domain-containing protein At3g58270 OS=Arabidopsis thaliana OX=3702 GN=At3g58270 PE=2 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 3.3e-10
Identity = 37/121 (30.58%), Postives = 64/121 (52.89%), Query Frame = 0

Query: 51  KKCESGDFEIDGYKWKLILYPNGDDKVDDHISLYLAISSKDNLPLGWEVKVIFRFFIFDQ 110
           +K  S +F +DG KW+L+ +P G+    + +SLYLA++  + LP GW     F F + +Q
Sbjct: 22  RKNYSDEFFVDGCKWRLLAFPKGNGV--EKLSLYLAVAGSEFLPDGWRRHAYFHFSVVNQ 81

Query: 111 IKDNYLTIQDGKVRKYSKMKSEHGFSQLVSHKAFNKASNGLLVDNCCTFGVEVSLLKPSN 170
           + D     ++ K   +    S+ GF+ ++S K  +    G LV+      V+VS+L+   
Sbjct: 82  LSDELSQARETK-NWFDASTSDWGFTSMLSLKKLHDKDGGFLVNGELKIVVDVSVLEVIG 139

Query: 171 K 172
           K
Sbjct: 142 K 139

BLAST of Spg002461 vs. ExPASy TrEMBL
Match: A0A6J1JFK1 (uncharacterized protein LOC111486429 OS=Cucurbita maxima OX=3661 GN=LOC111486429 PE=4 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 6.7e-131
Identity = 234/313 (74.76%), Postives = 265/313 (84.66%), Query Frame = 0

Query: 6   DNMLYEDGPVKDEPQHEITRSRRVDPPADYTFRINSYSVLSEIGMKKCESGDFEIDGYKW 65
           DNM Y+D P+K E Q EITR+ RVDPPA+Y F+INSYS LSEIGMKKCESG F +DGY W
Sbjct: 3   DNMSYDD-PIKHEQQPEITRTTRVDPPANYIFQINSYSTLSEIGMKKCESGHFAVDGYNW 62

Query: 66  KLILYPNGDDKVDDHISLYLAISSKDNLPLGWEVKVIFRFFIFDQIKDNYLTIQDGKVRK 125
           KLIL+PNGDDKV+DHISLYLAI+SKDNLPLGWEVKVIFRFF++DQIKDNYLTIQDGK+RK
Sbjct: 63  KLILFPNGDDKVEDHISLYLAITSKDNLPLGWEVKVIFRFFVYDQIKDNYLTIQDGKMRK 122

Query: 126 YSKMKSEHGFSQLVSHKAFNKASNGLLVDNCCTFGVEVSLLKPSNKGESLTIIKEPQQFE 185
           YSKMK+EHGF++ ++H AFN+ASNG LVDN CTFGVEVS+LK SNKGESLTI KEP Q  
Sbjct: 123 YSKMKTEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEP-QLG 182

Query: 186 SSYWSIYSFSTKTDESYVSNPFNVKGRQWRMKIYPNGFSSGKTTHLSLYLKLDSSETIPV 245
           S +WSI SFS K +E Y S PF VKGR+WR+ IYPNGF SGKT+H+SLYLKLDSSETIP 
Sbjct: 183 SFFWSISSFSNKNNEFYFSEPFTVKGRKWRLMIYPNGFLSGKTSHISLYLKLDSSETIPT 242

Query: 246 GKKIYAKFILSVYNRVDRKYIDKSARERWFDNTTYGSGFPEFLSRKDLS-NHLDGDVLYL 305
           GKKIYAKF LSVYN   +KY+D+S  E WFDNT YG GF EFLSR DL  +HL  DV YL
Sbjct: 243 GKKIYAKFFLSVYNFGAKKYVDQS-NEYWFDNTAYGHGFHEFLSRNDLQPHHLQNDVFYL 302

Query: 306 KARIVAMSTVEEF 318
           KARIVAMS V++F
Sbjct: 303 KARIVAMSVVQDF 312

BLAST of Spg002461 vs. ExPASy TrEMBL
Match: A0A6J1FUL4 (speckle-type POZ protein-like A OS=Cucurbita moschata OX=3662 GN=LOC111447016 PE=4 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 1.3e-129
Identity = 229/313 (73.16%), Postives = 267/313 (85.30%), Query Frame = 0

Query: 6   DNMLYEDGPVKDEPQHEITRSRRVDPPADYTFRINSYSVLSEIGMKKCESGDFEIDGYKW 65
           DNM Y+D P+K E Q EITR+RR +PPA+YTF+INSYS LSEIGMKKCESG+F +DG+ W
Sbjct: 3   DNMSYDD-PIKHEQQPEITRTRRAEPPANYTFQINSYSTLSEIGMKKCESGNFAVDGFNW 62

Query: 66  KLILYPNGDDKVDDHISLYLAISSKDNLPLGWEVKVIFRFFIFDQIKDNYLTIQDGKVRK 125
           KLILYPNGDD+V+DHISLYLAI+SKDNLPLGWEVKVIFRFF++D+IKDNYLT+QDGK+RK
Sbjct: 63  KLILYPNGDDEVEDHISLYLAITSKDNLPLGWEVKVIFRFFVYDRIKDNYLTVQDGKMRK 122

Query: 126 YSKMKSEHGFSQLVSHKAFNKASNGLLVDNCCTFGVEVSLLKPSNKGESLTIIKEPQQFE 185
           YSKMKSEHGF++ ++H AFN+ASNG LVDN CTFGVEVS+LK SNKGESLTI KEP Q  
Sbjct: 123 YSKMKSEHGFTKFLAHNAFNQASNGFLVDNGCTFGVEVSVLKASNKGESLTIFKEP-QLG 182

Query: 186 SSYWSIYSFSTKTDESYVSNPFNVKGRQWRMKIYPNGFSSGKTTHLSLYLKLDSSETIPV 245
           S +WSI SFS KT+E Y+S PF VKGR+WR+ IYPNG SSGK +H+SLYLKLDSSET+P 
Sbjct: 183 SFFWSISSFSNKTNEFYLSEPFTVKGRKWRLMIYPNGLSSGKMSHISLYLKLDSSETVPT 242

Query: 246 GKKIYAKFILSVYNRVDRKYIDKSARERWFDNTTYGSGFPEFLSRKDL-SNHLDGDVLYL 305
           GKKIYAKF LSVY+   ++Y+D+S  E WFDNTTYG GF EFLSR DL  +HL  D  YL
Sbjct: 243 GKKIYAKFFLSVYDFGAKRYVDQS-NEHWFDNTTYGYGFHEFLSRNDLRPHHLQNDGFYL 302

Query: 306 KARIVAMSTVEEF 318
           KARI AMS VE+F
Sbjct: 303 KARIAAMSVVEDF 312

BLAST of Spg002461 vs. ExPASy TrEMBL
Match: A0A5A7TNH7 (Ubiquitin carboxyl-terminal hydrolase 12-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00430 PE=4 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 5.3e-128
Identity = 227/316 (71.84%), Postives = 270/316 (85.44%), Query Frame = 0

Query: 5   DDNMLYEDGPVKDEPQHEIT-RSRRVDPPADYTFRINSYSVLSEIGMKKCESGDFEIDGY 64
           DDNM Y++  +KDE QHEIT +SRRVDPP+ Y F+I+SYSVLSEI MKKCESGDFE+DGY
Sbjct: 2   DDNMSYDES-IKDERQHEITIKSRRVDPPSHYAFQIDSYSVLSEIEMKKCESGDFEVDGY 61

Query: 65  KWKLILYPNGDDKVDDHISLYLAISSKD--NLPLGWEVKVIFRFFIFDQIKDNYLTIQDG 124
            WKLILYPNG+ +V+DHISL+LAIS+ D  NLPLGWEV+VIFRFFIFDQI+DNYLTIQDG
Sbjct: 62  MWKLILYPNGNGEVEDHISLFLAISTNDNNNLPLGWEVRVIFRFFIFDQIRDNYLTIQDG 121

Query: 125 KVRKYSKMKSEHGFSQLVSHKAFNKASNGLLVDNCCTFGVEVSLLKPSNKGESLTIIKEP 184
           K+RKYSKMKSEHGF+ L+SH  F+KASNG LV+N CTFGVEVS+LK SNKGE LTI +EP
Sbjct: 122 KMRKYSKMKSEHGFTHLISHNVFSKASNGFLVNNSCTFGVEVSILKASNKGERLTIFREP 181

Query: 185 QQFESSYWSIYSFSTKTDESYVSNPFNVKGRQWRMKIYPNGFSSGKTTHLSLYLKLDSSE 244
           QQ ++ +W++YSFS KT++SY+S PFNVKGR+WRM+IYPNG S GKT+H+SLYLKLD+SE
Sbjct: 182 QQ-DTYFWTLYSFSAKTNQSYISEPFNVKGRKWRMEIYPNGNSLGKTSHISLYLKLDNSE 241

Query: 245 TIPVGKKIYAKFILSVYNRVDRKYIDKSARERWFDNTTYGSGFPEFLSRKDLSNHLDGDV 304
           TIP+GKKIYAKF+L VYN   +KYIDKS  E WF    +G GF EFLSR+++S H   D 
Sbjct: 242 TIPLGKKIYAKFLLGVYNFSAKKYIDKS-YEHWFKTPGHGDGFDEFLSRREISTHSQNDA 301

Query: 305 LYLKARIVAMSTVEEF 318
            YLKARIVAMSTV+EF
Sbjct: 302 FYLKARIVAMSTVDEF 314

BLAST of Spg002461 vs. ExPASy TrEMBL
Match: A0A1S3BYG6 (uncharacterized protein LOC103494460 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494460 PE=4 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 5.3e-128
Identity = 227/316 (71.84%), Postives = 270/316 (85.44%), Query Frame = 0

Query: 5   DDNMLYEDGPVKDEPQHEIT-RSRRVDPPADYTFRINSYSVLSEIGMKKCESGDFEIDGY 64
           DDNM Y++  +KDE QHEIT +SRRVDPP+ Y F+I+SYSVLSEI MKKCESGDFE+DGY
Sbjct: 2   DDNMSYDES-IKDERQHEITIKSRRVDPPSHYAFQIDSYSVLSEIEMKKCESGDFEVDGY 61

Query: 65  KWKLILYPNGDDKVDDHISLYLAISSKD--NLPLGWEVKVIFRFFIFDQIKDNYLTIQDG 124
            WKLILYPNG+ +V+DHISL+LAIS+ D  NLPLGWEV+VIFRFFIFDQI+DNYLTIQDG
Sbjct: 62  MWKLILYPNGNGEVEDHISLFLAISTNDNNNLPLGWEVRVIFRFFIFDQIRDNYLTIQDG 121

Query: 125 KVRKYSKMKSEHGFSQLVSHKAFNKASNGLLVDNCCTFGVEVSLLKPSNKGESLTIIKEP 184
           K+RKYSKMKSEHGF+ L+SH  F+KASNG LV+N CTFGVEVS+LK SNKGE LTI +EP
Sbjct: 122 KMRKYSKMKSEHGFTHLISHNVFSKASNGFLVNNSCTFGVEVSILKASNKGERLTIFREP 181

Query: 185 QQFESSYWSIYSFSTKTDESYVSNPFNVKGRQWRMKIYPNGFSSGKTTHLSLYLKLDSSE 244
           QQ ++ +W++YSFS KT++SY+S PFNVKGR+WRM+IYPNG S GKT+H+SLYLKLD+SE
Sbjct: 182 QQ-DTYFWTLYSFSAKTNQSYISEPFNVKGRKWRMEIYPNGNSLGKTSHISLYLKLDNSE 241

Query: 245 TIPVGKKIYAKFILSVYNRVDRKYIDKSARERWFDNTTYGSGFPEFLSRKDLSNHLDGDV 304
           TIP+GKKIYAKF+L VYN   +KYIDKS  E WF    +G GF EFLSR+++S H   D 
Sbjct: 242 TIPLGKKIYAKFLLGVYNFSAKKYIDKS-YEHWFKTPGHGDGFDEFLSRREISTHSQNDA 301

Query: 305 LYLKARIVAMSTVEEF 318
            YLKARIVAMSTV+EF
Sbjct: 302 FYLKARIVAMSTVDEF 314

BLAST of Spg002461 vs. ExPASy TrEMBL
Match: A0A0A0KUE1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G023030 PE=4 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 1.6e-127
Identity = 225/315 (71.43%), Postives = 267/315 (84.76%), Query Frame = 0

Query: 5   DDNMLYEDGPVKDEPQHEIT-RSRRVDPPADYTFRINSYSVLSEIGMKKCESGDFEIDGY 64
           DDNM Y+D    DE QHEIT +SRRVDPP+ Y F+I+SYSVLS+I MKKCESGDFE+DGY
Sbjct: 2   DDNMSYDDTTKIDERQHEITIKSRRVDPPSHYAFQIDSYSVLSQIEMKKCESGDFEVDGY 61

Query: 65  KWKLILYPNGDDKVDDHISLYLAISSKD-NLPLGWEVKVIFRFFIFDQIKDNYLTIQDGK 124
           KWKLILYPNG+++V+DHISL+LA+S+ D NLPLGWE++VIFRFFIFDQI+DNYLTIQDGK
Sbjct: 62  KWKLILYPNGNEEVEDHISLFLAVSTNDNNLPLGWELRVIFRFFIFDQIRDNYLTIQDGK 121

Query: 125 VRKYSKMKSEHGFSQLVSHKAFNKASNGLLVDNCCTFGVEVSLLKPSNKGESLTIIKEPQ 184
           +RKYSKMKSEHGF+ L+SH  FNKAS+G LV NCCTFGVEVS+LK SNKGE LTI+KEPQ
Sbjct: 122 MRKYSKMKSEHGFTHLISHNVFNKASSGFLVSNCCTFGVEVSILKASNKGERLTILKEPQ 181

Query: 185 QFESSYWSIYSFSTKTDESYVSNPFNVKGRQWRMKIYPNGFSSGKTTHLSLYLKLDSSET 244
           Q ++ +W++YSFS      Y+S PFNVKGR+WRM++YP+G S GKT+H+SLYLKLDSSET
Sbjct: 182 Q-DTYFWTLYSFSALKQPFYISEPFNVKGRKWRMEVYPHGNSLGKTSHISLYLKLDSSET 241

Query: 245 IPVGKKIYAKFILSVYNRVDRKYIDKSARERWFDNTTYGSGFPEFLSRKDLSNHLDGDVL 304
           IP+GKKIYAKFIL VYN   +KYIDKS  E W+    +G+GF EFLSRK++S H   D  
Sbjct: 242 IPLGKKIYAKFILGVYNFSAKKYIDKS-YEHWYKTPGHGNGFDEFLSRKEISTHSQNDAF 301

Query: 305 YLKARIVAMSTVEEF 318
           YLKARIVAMSTVEEF
Sbjct: 302 YLKARIVAMSTVEEF 314

BLAST of Spg002461 vs. TAIR 10
Match: AT3G17380.1 (TRAF-like family protein )

HSP 1 Score: 200.3 bits (508), Expect = 2.3e-51
Identity = 102/272 (37.50%), Postives = 162/272 (59.56%), Query Frame = 0

Query: 22  EITRSRRVDPPADYTFRINSYSVLSEIGMKKCESGDFEIDGYKWKLILYPNGD--DKVDD 81
           EI +S    PP  Y  +I S+S+L++  +++ E+  FE  GYKWKL+LYPNG+      D
Sbjct: 9   EIIKSISDAPPTHYMVKIESFSLLTKHAIERYETESFEAGGYKWKLVLYPNGNKSKNTKD 68

Query: 82  HISLYLAISSKDNLPLGWEVKVIFRFFIFDQIKDNYLTIQDGKVRKYSKMKSEHGFSQLV 141
           H+S+YL+++   +L  GWEV  +FR ++ DQ KDNYL +Q G  R++  +K E GF + +
Sbjct: 69  HVSVYLSLADSSSLSPGWEVYAVFRLYLLDQNKDNYLILQ-GNERRFHSVKREWGFDKFI 128

Query: 142 SHKAFNKASNGLLVDNCCTFGVEVSLLKP--SNKGESLTIIKEPQQFESSYWSIYSFSTK 201
               F+ ASNG L+++ C FG +V + K   S +GE L++IK+        W I +FS  
Sbjct: 129 PTGTFSDASNGYLMEDTCMFGADVFVSKERRSGRGECLSMIKDATS-SKHVWKIENFSKL 188

Query: 202 TDESYVSNPFNVKGRQWRMKIYPNGFSSGKTTHLSLYLKLDSSETIPVGKKIYAKFILSV 261
             ESY SN F    R+W+++ YP G   G  THLS+YL L   ETI  G KI+ +F + +
Sbjct: 189 DKESYDSNAFFAGDRKWKIEFYPTGTKQGTGTHLSIYLTLVDPETISDGTKIFVEFTIRI 248

Query: 262 YNRVDRKYIDKSARERWFDNTTYGSGFPEFLS 290
           ++++  ++I      +WF  ++   G+ +++S
Sbjct: 249 FDQLQGRHIAGKV-TKWFSRSSSEHGWVKYVS 277

BLAST of Spg002461 vs. TAIR 10
Match: AT5G26280.1 (TRAF-like family protein )

HSP 1 Score: 160.6 bits (405), Expect = 2.1e-39
Identity = 99/299 (33.11%), Postives = 152/299 (50.84%), Query Frame = 0

Query: 23  ITRSRRVDPPADYTFRINSYSVLSEIGMKKCESGDFEIDGYKWKLILYPNG--DDKVDDH 82
           +TR  R + P+     I S+SV+   G +  ES  FE  GYKW+L+LY NG  +D  +DH
Sbjct: 50  VTRELREERPSSKIVTITSFSVIKGRG-EPYESSVFEAAGYKWRLVLYVNGNKNDGGNDH 109

Query: 83  ISLYLAISSKDNLPLGWEVKVIFRFFIFDQIKDNYLTIQDGKVRKYSKMKSEHGFSQLVS 142
           ISLY  I   ++LPLGWEV V  + F+ +     YLT+ DG V++Y+  K E GF QL+ 
Sbjct: 110 ISLYARIEETNSLPLGWEVNVDLKLFVHNGKLHKYLTVTDGLVKRYNNAKKEWGFGQLIP 169

Query: 143 HKAFNKASNGLLVDNCCTFGVEVSLLKPSNKGESLTIIKEPQQFESSYWSIYSFSTKTDE 202
              F  A+ G L  +  +FG E+ ++KP+ + E +T I  P       W I  FST  D+
Sbjct: 170 RSTFYNANEGYLDQDTGSFGAEIFIVKPAQQQEKVTFISNPPN-NVFTWKILRFSTLEDK 229

Query: 203 SYVSNPFNVKGRQWRMKIYPNGFSSGKTTHLSLYLKLDSSETIPVGKKIYAKFILSVYNR 262
            Y S+ F V+ R WR+   P G   G+   L ++L     +   V    +    L + N+
Sbjct: 230 FYYSDDFLVEDRYWRLGFNPKGDGGGRPHALPIFLFAQGHKANAVATNTWGAVNLRLKNQ 289

Query: 263 --VDRKYIDKSARERWFD-NTTYGSGFPEFLSRKDLSN----HLDGDVLYLKARIVAMS 313
              + + I  +A   W+   + YG G    +   DL++    +L  D +  +A +V +S
Sbjct: 290 RSTNHRQIYSAA---WYPIGSGYGVGVNNIILLADLNDASKGYLVNDAIIFEAEMVKVS 343

BLAST of Spg002461 vs. TAIR 10
Match: AT5G26260.1 (TRAF-like family protein )

HSP 1 Score: 156.8 bits (395), Expect = 3.0e-38
Identity = 92/295 (31.19%), Postives = 147/295 (49.83%), Query Frame = 0

Query: 24  TRSRRVDPPADYTFRINSYSVLSEIGMKKCESGDFEIDGYKWKLILYPNGDDK--VDDHI 83
           TR  R + P+     I S+SV+ + G +  ES  FE  GYKW+L+LY  G+ K  +++HI
Sbjct: 52  TRELRDERPSSKIVTITSFSVIKDRG-EPYESSIFEAAGYKWRLVLYVKGNPKGGINNHI 111

Query: 84  SLYLAISSKDNLPLGWEVKVIFRFFIFDQIKDNYLTIQDGKVRKYSKMKSEHGFSQLVSH 143
           SLY  I   + LP GWEV V  + F+ ++    YL++ DG V++Y+  K E GF+QL+S 
Sbjct: 112 SLYARIEETETLPRGWEVNVDLKLFVHNRKLKKYLSVTDGTVKRYNDAKKEWGFTQLISL 171

Query: 144 KAFNKASNGLLVDNCCTFGVEVSLLKPSNKGESLTIIKEPQQFESSYWSIYSFSTKTDES 203
             F  A+ G LV +  +FG E+ ++ P+ K E +T I  P       W I  FST  D+ 
Sbjct: 172 PTFYNANEGYLVQDTASFGAEIFIVNPTEKQEKVTFISNPPD-NVFTWKILRFSTLEDKF 231

Query: 204 YVSNPFNVKGRQWRMKIYPNGFSSGKTTHLSLYLKLDSSETIPVGKKIYAKFILSVYNRV 263
           Y S+ F V  R WR+   P G   G+   L ++L     +   V    +    L + N+ 
Sbjct: 232 YYSDDFLVGDRYWRLGFNPKGSGGGRPHALPIFLYAQGHKANAVVTNTWGAVNLRLKNQR 291

Query: 264 DRKYIDKSARERWFDNTTYGSGFPEFLSRKDLSN----HLDGDVLYLKARIVAMS 313
              +    +   +   + YG G    +   +L +    ++  D +  +A +V +S
Sbjct: 292 SSNHKQLYSAAWYPIRSDYGVGVNNIILMSELKDASKGYMVNDAIIFEAEMVKVS 344

BLAST of Spg002461 vs. TAIR 10
Match: AT3G20370.1 (TRAF-like family protein )

HSP 1 Score: 152.1 bits (383), Expect = 7.3e-37
Identity = 100/299 (33.44%), Postives = 162/299 (54.18%), Query Frame = 0

Query: 31  PPADYTFRINSY-SVLSEIGMKKCESGDFEIDGYKWKLILYPNGD--DKVDDHISLYLAI 90
           PP+ Y+ ++ S+ ++L     +K  S  F + GY W L+++PNG+  D    ++SLY+AI
Sbjct: 84  PPSSYSLKMESFNTLLKSTYTEKYVSRPFSVGGYNWTLVVFPNGNKKDSGSGYLSLYVAI 143

Query: 91  SSKDNLPLG-WEVKVIFRFFIFDQIKDNYLTIQDGKVRKYSKMKSEHGFSQLVSHKAFNK 150
              DN  LG  E+    RF+IF++ +  Y TIQD  V K+S  K+  GFSQ++    F  
Sbjct: 144 ---DNSTLGQQEIYADLRFYIFNKNERKYFTIQDTDVWKFSVFKTMWGFSQVLPIDTFKD 203

Query: 151 ASNGLLVD-NCCTFGVEVSLLKPSNKGESLTIIKEPQQFESSYWSIYSFSTKTDESYVSN 210
            + G L D + C FGV+V++     K E L  + E        W+I  FST    SY+S 
Sbjct: 204 PTKGYLYDGDHCEFGVDVTMPSLYEKSE-LFSVTENFLNPRFTWTIRGFSTLLKNSYLSE 263

Query: 211 PFNVKGRQWRMKIYPNGFSSGKTTHLSLYLKLDSSETIPVGKKIYAKFILSVYNRVDRKY 270
            F++ GR W ++I P+G  +G+   LS+YL L+ +E     +KIY +  L   N+++   
Sbjct: 264 VFSIGGRSWNIQINPSGLGTGEGKALSMYLGLNVNEIFRPYEKIYVRAKLRALNQLNLSN 323

Query: 271 IDKSARERWFDNTTYGS---GFPEFLSRKDLSNHLDG----DVLYLKARIVAMSTVEEF 318
           I++   + W++   YG    GFPEF+    L++   G    DVL ++  + A+S+ + F
Sbjct: 324 IEREL-DIWYNGPGYGEYSWGFPEFIYFPYLTDSSKGFVKNDVLMVQVEMEAISSTKYF 377

BLAST of Spg002461 vs. TAIR 10
Match: AT2G15710.1 (TRAF-like family protein )

HSP 1 Score: 144.8 bits (364), Expect = 1.2e-34
Identity = 92/281 (32.74%), Postives = 150/281 (53.38%), Query Frame = 0

Query: 23  ITRSRRVDPPADYTFRINSYSVLSEIGMKKCESGDFEIDGYKWKLILYPNGD--DKVDDH 82
           I  + R  PP+ Y+ +IN    LS++   K ES  F   GY W+L++YP G+  DK    
Sbjct: 89  IVNNLREHPPSSYSLKINK---LSQLTFDKYESHRFLSGGYNWRLVIYPKGNEKDKGSGF 148

Query: 83  ISLYLAI-SSKDNLPLGWEVKVIFRFFIFDQIKDNYLTIQDGKVRKYSKMKSEHGFSQLV 142
           IS+Y+   ++K +     EV     FF++++ ++ Y TIQD +V++++ +++  G SQ++
Sbjct: 149 ISMYVEFDNTKVSSTSPMEVFAYIIFFVYNKKENKYFTIQDVEVKRFNALRTVWGLSQVL 208

Query: 143 SHKAFNKASNGLLVD-NCCTFGVEVSLLKPSNKGESLTIIKEPQQFESSYWSIYSFSTKT 202
           S + FN   NG   +   C FGV+V +  P  K E ++  ++    + S WS+  FS   
Sbjct: 209 SLETFNDLENGYTFEGEQCEFGVDVMVASPITKWEVVSFDEKLDILKFS-WSVKDFSVLK 268

Query: 203 DESYVSNPFNVKGRQWRMKIYPNGFSSGKTTHLSLYLKLDSSETIPVGKKIYAKFILSVY 262
           +E YVS  F++ GR W +++YP G        LS++L+L  SET+ V +KIY    L V 
Sbjct: 269 EEFYVSERFSMGGRLWDLQMYPKGDPRRDKKWLSIFLRLSGSETLTVDEKIYVIAHLRVL 328

Query: 263 NRVDRKYIDKSARERWFDNTTYGSGFPEFLSRKDL-SNHLD 299
           + +            WF +   G G+ EFLS   L  ++LD
Sbjct: 329 DPLG----------NWFRDRNKGWGYLEFLSFSKLRKSYLD 355

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038902857.12.4e-13577.07MATH domain and coiled-coil domain-containing protein At3g58400-like [Benincasa ... [more]
KAG6600198.13.3e-13274.52Ubiquitin carboxyl-terminal hydrolase 12, partial [Cucurbita argyrosperma subsp.... [more]
XP_023529004.14.3e-13275.08speckle-type POZ protein-like A [Cucurbita pepo subsp. pepo][more]
XP_022989347.11.4e-13074.76uncharacterized protein LOC111486429 [Cucurbita maxima][more]
XP_022941740.12.6e-12973.16speckle-type POZ protein-like A [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9M2J13.9e-1130.33MATH domain and coiled-coil domain-containing protein At3g58250 OS=Arabidopsis t... [more]
Q9M2H95.1e-1129.01MATH domain and coiled-coil domain-containing protein At3g58370 OS=Arabidopsis t... [more]
Q84WU21.1e-1026.62Ubiquitin C-terminal hydrolase 13 OS=Arabidopsis thaliana OX=3702 GN=UBP13 PE=1 ... [more]
Q9FPT11.5e-1026.55Ubiquitin C-terminal hydrolase 12 OS=Arabidopsis thaliana OX=3702 GN=UBP12 PE=1 ... [more]
Q9M2I93.3e-1030.58MATH domain and coiled-coil domain-containing protein At3g58270 OS=Arabidopsis t... [more]
Match NameE-valueIdentityDescription
A0A6J1JFK16.7e-13174.76uncharacterized protein LOC111486429 OS=Cucurbita maxima OX=3661 GN=LOC111486429... [more]
A0A6J1FUL41.3e-12973.16speckle-type POZ protein-like A OS=Cucurbita moschata OX=3662 GN=LOC111447016 PE... [more]
A0A5A7TNH75.3e-12871.84Ubiquitin carboxyl-terminal hydrolase 12-like isoform X2 OS=Cucumis melo var. ma... [more]
A0A1S3BYG65.3e-12871.84uncharacterized protein LOC103494460 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A0A0KUE11.6e-12771.43Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G023030 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G17380.12.3e-5137.50TRAF-like family protein [more]
AT5G26280.12.1e-3933.11TRAF-like family protein [more]
AT5G26260.13.0e-3831.19TRAF-like family protein [more]
AT3G20370.17.3e-3733.44TRAF-like family protein [more]
AT2G15710.11.2e-3432.74TRAF-like family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002083MATH/TRAF domainSMARTSM00061math_3coord: 37..144
e-value: 1.9E-4
score: 30.8
coord: 189..293
e-value: 9.2E-13
score: 58.4
IPR002083MATH/TRAF domainPFAMPF00917MATHcoord: 43..164
e-value: 2.2E-9
score: 37.4
coord: 193..308
e-value: 4.1E-12
score: 46.3
IPR002083MATH/TRAF domainPROSITEPS50144MATHcoord: 32..163
score: 23.689407
IPR002083MATH/TRAF domainPROSITEPS50144MATHcoord: 184..308
score: 24.916744
IPR002083MATH/TRAF domainCDDcd00121MATHcoord: 35..163
e-value: 1.86456E-24
score: 93.983
IPR002083MATH/TRAF domainCDDcd00121MATHcoord: 189..308
e-value: 3.26252E-25
score: 96.2942
IPR008974TRAF-likeGENE3D2.60.210.10Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain Acoord: 176..314
e-value: 1.7E-26
score: 94.8
coord: 17..171
e-value: 7.3E-37
score: 128.5
NoneNo IPR availablePANTHERPTHR46162:SF40UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 12-LIKEcoord: 26..315
NoneNo IPR availablePANTHERPTHR46162TRAF-LIKE FAMILY PROTEINcoord: 26..315
NoneNo IPR availableSUPERFAMILY49599TRAF domain-likecoord: 33..169
NoneNo IPR availableSUPERFAMILY49599TRAF domain-likecoord: 188..313

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg002461.1Spg002461.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
molecular_function GO:0016301 kinase activity
molecular_function GO:0005515 protein binding