Homology
BLAST of Spg002423 vs. NCBI nr
Match:
XP_022945852.1 (cytochrome P450 714C2-like [Cucurbita moschata])
HSP 1 Score: 886.7 bits (2290), Expect = 1.0e-253
Identity = 433/512 (84.57%), Postives = 468/512 (91.41%), Query Frame = 0
Query: 34 VSIMVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAINGGSF 93
V +MVLFVKVV+SWW+LP+LAHQKLKRNGF GP PSFPLGNIAEMK+ KA+AAING +
Sbjct: 14 VLVMVLFVKVVVSWWVLPVLAHQKLKRNGFRGPPPSFPLGNIAEMKRIRKASAAINGDAH 73
Query: 94 ASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGW 153
+ S+ VSHDIHS VFPHFAQWQASYGKKF+YWLGTEPFLYIADP F+KKISEAVM KGW
Sbjct: 74 SFPSSKVSHDIHSTVFPHFAQWQASYGKKFIYWLGTEPFLYIADPHFMKKISEAVMAKGW 133
Query: 154 GKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKW 213
GKPAVFRNDR PMFGDGLVMTEGDDW+RHRHILTPAFNPANLKAMTKFMVESTT+MLNKW
Sbjct: 134 GKPAVFRNDRRPMFGDGLVMTEGDDWVRHRHILTPAFNPANLKAMTKFMVESTTKMLNKW 193
Query: 214 SSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGV 273
SSLVNSA EIDVEREITATAGDIIA+ASFGI N GGRDAF+ LRALQFTLFQNSTYVGV
Sbjct: 194 SSLVNSAGGEIDVEREITATAGDIIAEASFGICNDGGRDAFDYLRALQFTLFQNSTYVGV 253
Query: 274 PFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGGG 333
PFS LF CP RTL+AKRLGA IDRLFLS++S+RK S++ SSSDLLSRLIE +++ GG
Sbjct: 254 PFSSLFFCPGRTLSAKRLGAAIDRLFLSLVSKRKLSQD--SSSDLLSRLIEGEKSTGDGG 313
Query: 334 KMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEIDF 393
KM LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEWQ+ILREEIKEVIGDKEIDF
Sbjct: 314 KMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDILREEIKEVIGDKEIDF 373
Query: 394 AMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDPTL 453
MLSGL+KMG VF+EVLRLYPSAPNIQRQAKGDI VG +TIPKGTNIWID+V+MH DP L
Sbjct: 374 NMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIDVGSMTIPKGTNIWIDIVAMHRDPDL 433
Query: 454 WGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRFS 513
WG+D NEFKPERF +DSI GGCNHKMGYLPFGFGGRMCIG+NLSSMEYKIVLTLILSRFS
Sbjct: 434 WGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKNLSSMEYKIVLTLILSRFS 493
Query: 514 LSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 546
LSLSP+Y+HSPA LLSLRPAHGLPLIV PL P
Sbjct: 494 LSLSPNYAHSPATLLSLRPAHGLPLIVTPLHP 523
BLAST of Spg002423 vs. NCBI nr
Match:
KAG6574181.1 (Cytochrome P450 714C2, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 886.3 bits (2289), Expect = 1.3e-253
Identity = 433/512 (84.57%), Postives = 468/512 (91.41%), Query Frame = 0
Query: 34 VSIMVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAINGGSF 93
V +MVLFVKVV+SWW+LP+LAHQKLKRNGF GP PSFPLGNIAEMK+ KA+AAING +
Sbjct: 14 VLVMVLFVKVVVSWWVLPVLAHQKLKRNGFRGPPPSFPLGNIAEMKRIRKASAAINGDAH 73
Query: 94 ASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGW 153
+ S+ VSHDIHS VFPHFAQWQASYGKKF+YWLGTEPFLYIADP F+KKISEAVM KGW
Sbjct: 74 SFPSSKVSHDIHSTVFPHFAQWQASYGKKFIYWLGTEPFLYIADPHFMKKISEAVMAKGW 133
Query: 154 GKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKW 213
GKPAVFRNDR PMFGDGLVMTEGDDW+RHRHILTPAFNPANLKAMTKFMVESTT+MLNKW
Sbjct: 134 GKPAVFRNDRRPMFGDGLVMTEGDDWVRHRHILTPAFNPANLKAMTKFMVESTTKMLNKW 193
Query: 214 SSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGV 273
SSLVNSA EIDVEREITATAGDIIA+ASFGI N GGRDAF+ LRALQFTLFQNSTYVGV
Sbjct: 194 SSLVNSAGGEIDVEREITATAGDIIAKASFGICNDGGRDAFDYLRALQFTLFQNSTYVGV 253
Query: 274 PFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGGG 333
PFS LF CP RTL+AKRLGA IDRLFLS++S+RK S++ SSSDLLSRLIE +++ GG
Sbjct: 254 PFSSLFFCPGRTLSAKRLGAAIDRLFLSLVSKRKLSQD--SSSDLLSRLIEGEKSTGDGG 313
Query: 334 KMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEIDF 393
KM LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEWQ+ILREEIKEVIGDKEIDF
Sbjct: 314 KMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDILREEIKEVIGDKEIDF 373
Query: 394 AMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDPTL 453
MLSGL+KMG VF+EVLRLYPSAPNIQRQAKGDI VG +TIPKGTNIWID+V+MH DP L
Sbjct: 374 NMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIDVGSMTIPKGTNIWIDIVAMHRDPDL 433
Query: 454 WGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRFS 513
WG+D NEFKPERF +DSI GGCNHKMGYLPFGFGGRMCIG+NLSSMEYKIVLTLILSRFS
Sbjct: 434 WGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKNLSSMEYKIVLTLILSRFS 493
Query: 514 LSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 546
LSLSP+Y+HSPA LLSLRPAHGLPLIV PL P
Sbjct: 494 LSLSPNYAHSPATLLSLRPAHGLPLIVTPLHP 523
BLAST of Spg002423 vs. NCBI nr
Match:
XP_022968014.1 (cytochrome P450 714C2-like [Cucurbita maxima])
HSP 1 Score: 881.3 bits (2276), Expect = 4.2e-252
Identity = 434/512 (84.77%), Postives = 467/512 (91.21%), Query Frame = 0
Query: 34 VSIMVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAINGGSF 93
V IMVLFVKVV+SWW+LP+LAHQKLKRNGF GP P+FPLGNIAEMK+ KA+AAING S
Sbjct: 14 VLIMVLFVKVVVSWWVLPVLAHQKLKRNGFRGPPPTFPLGNIAEMKRIRKASAAINGDSH 73
Query: 94 ASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGW 153
+ S+ VSHDIHS VFPHFAQWQASYGKKFVYWLGTEPFLYIADP F+K+IS AVM KGW
Sbjct: 74 SFPSSKVSHDIHSTVFPHFAQWQASYGKKFVYWLGTEPFLYIADPHFMKQISVAVMAKGW 133
Query: 154 GKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKW 213
GKPAVFRNDR PMFGDGLVMTEGD W+RHRHILTPAFNPANLKAMTKFMVESTT+MLN+W
Sbjct: 134 GKPAVFRNDRRPMFGDGLVMTEGDVWVRHRHILTPAFNPANLKAMTKFMVESTTKMLNEW 193
Query: 214 SSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGV 273
SSLVNSA EIDVEREITATAGDIIA+ASFGI NGGGRDAF+ LRALQFTLFQNSTYVGV
Sbjct: 194 SSLVNSASGEIDVEREITATAGDIIAKASFGICNGGGRDAFDYLRALQFTLFQNSTYVGV 253
Query: 274 PFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGGG 333
PFS LF CP RTLAAKRLGA IDRLFLS++SERK S+ +SSSDLLSRLIE Q++ G
Sbjct: 254 PFSSLFFCPGRTLAAKRLGAAIDRLFLSLVSERKLSQ--NSSSDLLSRLIEGQKSTGDVG 313
Query: 334 KMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEIDF 393
KM LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEWQ+ILREEIKEVIGDKEIDF
Sbjct: 314 KMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDILREEIKEVIGDKEIDF 373
Query: 394 AMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDPTL 453
MLSGL+KMG VF+EVLRLYPSAPNIQRQAKGDI+VG +TIPKGTNIWID+V+MH DP L
Sbjct: 374 NMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIEVGSMTIPKGTNIWIDIVAMHRDPDL 433
Query: 454 WGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRFS 513
WG+D NEFKPERF +DSI GGCNHKMGYLPFGFGGRMCIG++LSSMEYKIVLTLILSRFS
Sbjct: 434 WGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKSLSSMEYKIVLTLILSRFS 493
Query: 514 LSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 546
LSLSP+YSHSPA LLSLRPAHGLPLIV PL P
Sbjct: 494 LSLSPNYSHSPATLLSLRPAHGLPLIVTPLHP 523
BLAST of Spg002423 vs. NCBI nr
Match:
XP_023542056.1 (cytochrome P450 714C2-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 878.6 bits (2269), Expect = 2.7e-251
Identity = 431/512 (84.18%), Postives = 465/512 (90.82%), Query Frame = 0
Query: 34 VSIMVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAINGGSF 93
V +MVLF+KVV+SWW+LP+LA QKLKRNGF GP PSFPLGNIAEMK+ KA+AAING
Sbjct: 14 VLVMVLFLKVVVSWWVLPVLALQKLKRNGFRGPPPSFPLGNIAEMKRIRKASAAINGDPH 73
Query: 94 ASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGW 153
+ S+ VSHDIHS VFPHFAQWQASYGKKF+YWLGTEPFLYIADP F+KKISEAVM KGW
Sbjct: 74 SFPSSKVSHDIHSTVFPHFAQWQASYGKKFIYWLGTEPFLYIADPHFMKKISEAVMAKGW 133
Query: 154 GKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKW 213
GKPAVFRNDR PMFGDGLVMTEGDDW+RHRHILTPAFNPANLKAMTKFMVESTT+MLNKW
Sbjct: 134 GKPAVFRNDRRPMFGDGLVMTEGDDWVRHRHILTPAFNPANLKAMTKFMVESTTKMLNKW 193
Query: 214 SSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGV 273
SSLVNSA EIDVER ITATAGDIIA+ASFGI N GGRDAF+ LRALQFTLFQNSTYVGV
Sbjct: 194 SSLVNSAGGEIDVERGITATAGDIIAKASFGICNDGGRDAFDYLRALQFTLFQNSTYVGV 253
Query: 274 PFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGGG 333
PFS LF CP RTL+AKRLGA IDRLFLS++S+RK S++ SSSDLLSRLIE +++ GG
Sbjct: 254 PFSSLFFCPGRTLSAKRLGAAIDRLFLSLVSKRKLSQD--SSSDLLSRLIEGEKSTGDGG 313
Query: 334 KMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEIDF 393
KM LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEWQ+ILREEIKEVIGDKEIDF
Sbjct: 314 KMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDILREEIKEVIGDKEIDF 373
Query: 394 AMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDPTL 453
MLSGL+KMG VF+EVLRLYPSAPNIQRQAKGDI VG +TIPKGTNIWID+V+MH DP L
Sbjct: 374 NMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIDVGSMTIPKGTNIWIDIVAMHRDPDL 433
Query: 454 WGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRFS 513
WG+D NEFKPERF +DSI GGCNHKMGYLPFGFGGRMCIG+NLSSMEYKIVLTLILSRFS
Sbjct: 434 WGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKNLSSMEYKIVLTLILSRFS 493
Query: 514 LSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 546
LSLSP+YSHSPA LLSLRPAHGLPLIV PL P
Sbjct: 494 LSLSPNYSHSPATLLSLRPAHGLPLIVTPLHP 523
BLAST of Spg002423 vs. NCBI nr
Match:
XP_022141037.1 (cytokinin hydroxylase [Momordica charantia])
HSP 1 Score: 875.9 bits (2262), Expect = 1.8e-250
Identity = 435/523 (83.17%), Postives = 475/523 (90.82%), Query Frame = 0
Query: 33 RVSIMVLFVKVVISWWILPILAHQKLKRNGFE-GPSPSFPLGNIAEMKKTMK------AA 92
RVS++VL V+VV SWWILP+LAH+KLKRNGF GP P FPLGNI E+K TMK AA
Sbjct: 13 RVSLVVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAA 72
Query: 93 AAINGGSFA-SSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKI 152
AAINGG+ A SSS+N+SHDIHS VFP+FAQWQ SYGKKFVYWLGTEPFLYIA+PQFVK +
Sbjct: 73 AAINGGAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNM 132
Query: 153 SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVE 212
SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMT+ MV
Sbjct: 133 SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRLMVG 192
Query: 213 STTQMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTL 272
STT+ML++W+ ++S PE+DVEREITATAGDIIA+ASFGI + GRDAFN LR LQFTL
Sbjct: 193 STTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQFTL 252
Query: 273 FQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIE 332
F+NS+YVGVPFS +FL P RTL AKRLG+EIDRLFLS+ISERKQS + +S+SDLLSRLIE
Sbjct: 253 FRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPD-ASASDLLSRLIE 312
Query: 333 SQRNGS--GGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEI 392
SQRN S GK LTTRELVDECKTFFFGGHETTALAISWT+LLLATRPEWQ ILREEI
Sbjct: 313 SQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQTILREEI 372
Query: 393 KEVIGDKEIDFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWI 452
KEVIGD+EIDFAMLS L+KMGWVFNEVLRLYPSAPNIQRQAKGDIKVGD TIPKGTNIWI
Sbjct: 373 KEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTIPKGTNIWI 432
Query: 453 DVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYK 512
D+V+MHHDP+LWG+DVNEFKPERFEKDSIHGGC HKMGYLPFGFGGRMCIGRNLSSMEYK
Sbjct: 433 DIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGRNLSSMEYK 492
Query: 513 IVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 546
IVLTLI+SRFSLS+SPSYSHSPAILLSLRPAHGLPLI+KPL P
Sbjct: 493 IVLTLIISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPLHP 534
BLAST of Spg002423 vs. ExPASy Swiss-Prot
Match:
Q9ZW95 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)
HSP 1 Score: 330.1 bits (845), Expect = 4.7e-89
Identity = 185/518 (35.71%), Postives = 283/518 (54.63%), Query Frame = 0
Query: 34 VSIMVLFVKV----VISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAIN 93
V +M L ++V + +++ P + ++R G GP P GNI ++ K + +A
Sbjct: 12 VIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDISKMLSHSA--- 71
Query: 94 GGSFASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKI---SE 153
++ +++ H+I + PH+ W YGK+F+ W GTEP L + + + +K++
Sbjct: 72 ----SNDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKELLTKHN 131
Query: 154 AVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVEST 213
V GK W + + + G GL+M G+ W RH+ PAF LK K MVE T
Sbjct: 132 PVTGKSW----LQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVECT 191
Query: 214 TQMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQ 273
M + L E+++ E+ DII++ FG S G++ F+ L LQ Q
Sbjct: 192 KMMAER---LRKEVGEEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQ 251
Query: 274 NSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQ 333
+ ++ P S FL K L E++RL + +I RK S E SS L+
Sbjct: 252 ATRHLCFPGS-RFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLL 311
Query: 334 RNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVI 393
N K L + ++DECKTFFF GHETT+L ++WTL+LLA P WQ+ +R+E+++V
Sbjct: 312 LNQMDSNKNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQVC 371
Query: 394 GDKEI-DFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVV 453
G + LS L + V NE LRLYP A + R A DIK+GD+ IPKG +IWI V+
Sbjct: 372 GQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIPVL 431
Query: 454 SMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVL 513
++HH LWGED NEF PERF S H ++PF G R CIG+ + ME KI+L
Sbjct: 432 AIHHSNELWGEDANEFNPERFTTRSF-ASSRH---FMPFAAGPRNCIGQTFAMMEAKIIL 491
Query: 514 TLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPL 544
+++S+FS ++S +Y H+P ++L+++P +G+ L++KPL
Sbjct: 492 AMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPL 510
BLAST of Spg002423 vs. ExPASy Swiss-Prot
Match:
Q2QYH7 (Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 SV=1)
HSP 1 Score: 329.3 bits (843), Expect = 8.1e-89
Identity = 182/511 (35.62%), Postives = 277/511 (54.21%), Query Frame = 0
Query: 37 MVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAINGGSFASS 96
++LF V I W+ P QKL+ G GP PSF GNI EM++ + A + + A S
Sbjct: 20 ILLFSYVYIILWLRPERLRQKLRSQGVRGPKPSFLFGNIPEMRRIQQLAKSAHEQE-AGS 79
Query: 97 SNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGWGKP 156
++ S + + +FP+F W YG ++Y G+ L + DP VK+++ GKP
Sbjct: 80 TDMFSSNYVATLFPYFLHWSRVYGSIYLYSTGSIQVLNVTDPNMVKELANC-KSLDLGKP 139
Query: 157 AVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKWSSL 216
+ +R + G G++ + GD W+ R ++ P +K M M+E+ MLN W +
Sbjct: 140 CYLQKERGALLGMGILTSNGDLWVHQRKVIAPELFMERVKGMVNLMMEAAMSMLNSWKNE 199
Query: 217 V--NSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGVP 276
V EI V+ + + D+I++A FG S G++ F +R LQ + + S +GVP
Sbjct: 200 VEDRGGSAEIVVDEFLRTFSADVISRACFGSSFSEGKEIFIKIRQLQKAMAKQSMLIGVP 259
Query: 277 FSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGGGK 336
S +L L + I L L++ + + S + DLL +I+ GS G
Sbjct: 260 GS-RYLPTRSNRGIWNLDSSIRTLILNISKKYEHDSSTSVNKDLLHSIIQ----GSKDGP 319
Query: 337 MRLTTRE--LVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEID 396
T E +VD CK +F GHETT+ +W L+LLA+ EWQ+ R E ++ + +D
Sbjct: 320 FASCTPEDFIVDNCKNIYFAGHETTSTTAAWCLMLLASHHEWQSRARVESLDICQGRPLD 379
Query: 397 FAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDPT 456
F +L L+K+ V E LRLYP A + R+A D+K+G + IPKGTNIWI + H DP+
Sbjct: 380 FDILRKLKKLTMVIQETLRLYPPASFVAREALNDMKLGGIDIPKGTNIWIPIAMAHRDPS 439
Query: 457 LWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRF 516
+WG ++F P+RF + I G C Y+PFG G R C G+NL+ +E K+VL+L+LS+F
Sbjct: 440 VWGPSADKFDPDRF-ANGIAGACKPPHMYMPFGVGVRTCAGQNLAMVELKVVLSLLLSKF 499
Query: 517 SLSLSPSYSHSPAILLSLRPAHGLPLIVKPL 544
LSP+Y H PA L++ P G+PLI + L
Sbjct: 500 EFKLSPNYVHCPAFRLTIEPGKGVPLIFREL 522
BLAST of Spg002423 vs. ExPASy Swiss-Prot
Match:
Q9FF18 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)
HSP 1 Score: 327.4 bits (838), Expect = 3.1e-88
Identity = 184/527 (34.91%), Postives = 284/527 (53.89%), Query Frame = 0
Query: 35 SIMVLFVKVVI--------SWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAA 94
S++V+FV ++ +W+ P + +++ G GP P GNI E+ +
Sbjct: 8 SLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEI-------S 67
Query: 95 AINGGSFASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKI-- 154
A+ S + +++ HDI + PH+ W YGK+F+ W GT+P L + + + +K++
Sbjct: 68 AMVSQSASKDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLM 127
Query: 155 -SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMV 214
V G+ W + +N G GL+M G DW RH+ PAF LK + MV
Sbjct: 128 KHNGVSGRSWLQQQGTKN----FIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMV 187
Query: 215 ESTTQMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFT 274
E T++++ + V E+++ E+ DII++ FG S G++ FN L LQ
Sbjct: 188 ECTSKLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRR 247
Query: 275 LFQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSS----DLL 334
Q + ++ P S FL K L E++RL + +I R+ E SS DLL
Sbjct: 248 CAQATRHLCFPGS-RFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLL 307
Query: 335 SRLIESQRNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILR 394
L+ + ++DECKTFFF GHETTAL ++WT +LLA P WQ +R
Sbjct: 308 GLLLNEMDIDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVR 367
Query: 395 EEIKEVIGDKEI-DFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGT 454
EE++EV G + LS L + V NE LRLYP A + R A D+K+GD+TIPKG
Sbjct: 368 EEVREVFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGL 427
Query: 455 NIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMG--YLPFGFGGRMCIGRNL 514
+IWI V+++HH LWG+D N+F PERF GG G ++PF G R CIG+
Sbjct: 428 SIWIPVLAIHHSEELWGKDANQFNPERF------GGRPFASGRHFIPFAAGPRNCIGQQF 487
Query: 515 SSMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPL 544
+ ME KI+L ++S+F+ ++S +Y H+P ++L+++P +G+ +I+KPL
Sbjct: 488 ALMEAKIILATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516
BLAST of Spg002423 vs. ExPASy Swiss-Prot
Match:
B6SSW8 (Cytochrome P450 714B3 OS=Zea mays OX=4577 GN=CYP714B3 PE=2 SV=1)
HSP 1 Score: 310.5 bits (794), Expect = 3.9e-83
Identity = 176/504 (34.92%), Postives = 269/504 (53.37%), Query Frame = 0
Query: 48 WILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAINGGSFASSS--NNVSHDIH 107
W++P K + GP PSFP GN+A+M++ AA A + S S + HD
Sbjct: 32 WLVPQKVLAKFEDQKIGGPRPSFPYGNLADMREAAAAAKAARASARRSGSGGGGIVHDYR 91
Query: 108 SIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGWGKPAVFRNDREP 167
V P++ +W+ YG F Y +G FL+++ P V+ I+ V GK + + EP
Sbjct: 92 PAVLPYYEKWRKEYGPIFTYSMGNVVFLHVSRPDVVRDINLCV-SLDLGKSSYLKATHEP 151
Query: 168 MFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKWSSLV--NSAQPE 227
+FG G++ + G+ W+ R I+ P F +K M MV+S +L W V N +
Sbjct: 152 LFGGGILKSNGEAWLHQRKIIAPEFFLDKVKGMVDLMVDSAQPLLMSWEERVDRNGGITD 211
Query: 228 IDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGVPFSGLFLCPA 287
I ++ +I A + D+I++ FG S G++ F +R LQ + + V +GL P+
Sbjct: 212 IKIDDDIRAYSADVISRTCFGSSYIKGKEIFMKIRELQQAV--SKPNVLAEMTGLRFFPS 271
Query: 288 -RTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGGGKMRLTTREL 347
R A L ++ +L L ++ +E+ +LLS ++ S G + +
Sbjct: 272 MRNKQAWELHKQVRKLILEIV------KESGEDRNLLSAILHSASTSRVG--IAEAENFI 331
Query: 348 VDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEIDFAMLSGLRKM 407
VD CK+ +F GHE+TA+ +W L+LL PEWQN +REE+ EV + +D L ++ +
Sbjct: 332 VDNCKSIYFAGHESTAVTAAWCLMLLGLHPEWQNRVREEVHEVCRGQPVDSRSLQKMKNL 391
Query: 408 GWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDPTLWGEDVNEFK 467
V E LRLYP+ + RQA ++K+G V IPKG NI+I V +MH DP LWG DV EF
Sbjct: 392 TMVIQETLRLYPAGAFVSRQALQELKLGGVHIPKGVNIYIPVSTMHLDPELWGPDVKEFN 451
Query: 468 PERFE--KDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRFSLSLSPSY 527
PERF + +H YLPFG G R C+G+ + E KI+++LI+S+F L LSP Y
Sbjct: 452 PERFSDVRPQLH-------SYLPFGAGARTCLGQGFAMAELKILISLIVSKFVLKLSPHY 511
Query: 528 SHSPAILLSLRPAHGLPLIVKPLQ 545
HSP + L + P G+ L + +Q
Sbjct: 512 QHSPTLKLIVEPELGVDLTLTKVQ 517
BLAST of Spg002423 vs. ExPASy Swiss-Prot
Match:
Q7XHW5 (Cytochrome P450 714B1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714B1 PE=1 SV=2)
HSP 1 Score: 307.0 bits (785), Expect = 4.3e-82
Identity = 170/511 (33.27%), Postives = 273/511 (53.42%), Query Frame = 0
Query: 48 WILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAINGGSFASSSNNVSHDIHSI 107
W+ P L+R G GP+PSFP GN+A+M+ AAA ++ HD
Sbjct: 27 WLAPERLRAHLRRQGIGGPTPSFPYGNLADMRSHAAAAAGGKATGEGRQEGDIVHDYRQA 86
Query: 108 VFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGWGKPAVFRNDREPMF 167
VFP + W+ YG F Y +G FL+++ P V+++S V GK + + +P+F
Sbjct: 87 VFPFYENWRKQYGPVFTYSVGNMVFLHVSRPDIVRELSLCV-SLDLGKSSYMKATHQPLF 146
Query: 168 GDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKWSSLVNSA---QPEI 227
G+G++ + G+ W R ++ P F P +K M MV+S +++ W ++ + ++
Sbjct: 147 GEGILKSNGNAWAHQRKLIAPEFFPDKVKGMVDLMVDSAQVLVSSWEDRIDRSGGNALDL 206
Query: 228 DVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGVPFSGL-FLCP- 287
++ +I A + D+I++ FG S G+ F+ +R LQ T+ + +GL FL P
Sbjct: 207 MIDDDIRAYSADVISRTCFGSSYVKGKQIFDMIRELQKTVSTKKQNLLAEMTGLSFLFPK 266
Query: 288 ARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGGGKMRLTTRE- 347
A AA RL + L L ++ E + + +LLS ++ S R G GGG E
Sbjct: 267 ASGRAAWRLNGRVRALILDLVGENGEED----GGNLLSAMLRSARGGGGGGGEVAAAAED 326
Query: 348 -LVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVI---GDKEIDFAMLS 407
+VD CK +F G+E+TA+ +W L+LLA PEWQ+ +R+E++ G + DF L
Sbjct: 327 FVVDNCKNIYFAGYESTAVTAAWCLMLLALHPEWQDRVRDEVQAACCGGGGRSPDFPALQ 386
Query: 408 GLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDPTLWGED 467
++ + V E LRLYP+ + RQA ++ +G V +P+G NI++ V ++H D LWG
Sbjct: 387 KMKNLTMVIQETLRLYPAGAVVSRQALRELSLGGVRVPRGVNIYVPVSTLHLDAELWGGG 446
Query: 468 VN--EFKPERF--EKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRFS 527
EF P RF + +H YLPFG G R C+G+ + E K++L+L+L RF
Sbjct: 447 AGAAEFDPARFADARPPLH-------AYLPFGAGARTCLGQTFAMAELKVLLSLVLCRFE 506
Query: 528 LSLSPSYSHSPAILLSLRPAHGLPLIVKPLQ 545
++LSP Y HSPA L + HG+ L++K ++
Sbjct: 507 VALSPEYVHSPAHKLIVEAEHGVRLVLKKVR 525
BLAST of Spg002423 vs. ExPASy TrEMBL
Match:
A0A6J1G224 (cytochrome P450 714C2-like OS=Cucurbita moschata OX=3662 GN=LOC111449972 PE=3 SV=1)
HSP 1 Score: 886.7 bits (2290), Expect = 4.8e-254
Identity = 433/512 (84.57%), Postives = 468/512 (91.41%), Query Frame = 0
Query: 34 VSIMVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAINGGSF 93
V +MVLFVKVV+SWW+LP+LAHQKLKRNGF GP PSFPLGNIAEMK+ KA+AAING +
Sbjct: 14 VLVMVLFVKVVVSWWVLPVLAHQKLKRNGFRGPPPSFPLGNIAEMKRIRKASAAINGDAH 73
Query: 94 ASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGW 153
+ S+ VSHDIHS VFPHFAQWQASYGKKF+YWLGTEPFLYIADP F+KKISEAVM KGW
Sbjct: 74 SFPSSKVSHDIHSTVFPHFAQWQASYGKKFIYWLGTEPFLYIADPHFMKKISEAVMAKGW 133
Query: 154 GKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKW 213
GKPAVFRNDR PMFGDGLVMTEGDDW+RHRHILTPAFNPANLKAMTKFMVESTT+MLNKW
Sbjct: 134 GKPAVFRNDRRPMFGDGLVMTEGDDWVRHRHILTPAFNPANLKAMTKFMVESTTKMLNKW 193
Query: 214 SSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGV 273
SSLVNSA EIDVEREITATAGDIIA+ASFGI N GGRDAF+ LRALQFTLFQNSTYVGV
Sbjct: 194 SSLVNSAGGEIDVEREITATAGDIIAEASFGICNDGGRDAFDYLRALQFTLFQNSTYVGV 253
Query: 274 PFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGGG 333
PFS LF CP RTL+AKRLGA IDRLFLS++S+RK S++ SSSDLLSRLIE +++ GG
Sbjct: 254 PFSSLFFCPGRTLSAKRLGAAIDRLFLSLVSKRKLSQD--SSSDLLSRLIEGEKSTGDGG 313
Query: 334 KMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEIDF 393
KM LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEWQ+ILREEIKEVIGDKEIDF
Sbjct: 314 KMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDILREEIKEVIGDKEIDF 373
Query: 394 AMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDPTL 453
MLSGL+KMG VF+EVLRLYPSAPNIQRQAKGDI VG +TIPKGTNIWID+V+MH DP L
Sbjct: 374 NMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIDVGSMTIPKGTNIWIDIVAMHRDPDL 433
Query: 454 WGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRFS 513
WG+D NEFKPERF +DSI GGCNHKMGYLPFGFGGRMCIG+NLSSMEYKIVLTLILSRFS
Sbjct: 434 WGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKNLSSMEYKIVLTLILSRFS 493
Query: 514 LSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 546
LSLSP+Y+HSPA LLSLRPAHGLPLIV PL P
Sbjct: 494 LSLSPNYAHSPATLLSLRPAHGLPLIVTPLHP 523
BLAST of Spg002423 vs. ExPASy TrEMBL
Match:
A0A6J1HYD5 (cytochrome P450 714C2-like OS=Cucurbita maxima OX=3661 GN=LOC111467387 PE=3 SV=1)
HSP 1 Score: 881.3 bits (2276), Expect = 2.0e-252
Identity = 434/512 (84.77%), Postives = 467/512 (91.21%), Query Frame = 0
Query: 34 VSIMVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAINGGSF 93
V IMVLFVKVV+SWW+LP+LAHQKLKRNGF GP P+FPLGNIAEMK+ KA+AAING S
Sbjct: 14 VLIMVLFVKVVVSWWVLPVLAHQKLKRNGFRGPPPTFPLGNIAEMKRIRKASAAINGDSH 73
Query: 94 ASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGW 153
+ S+ VSHDIHS VFPHFAQWQASYGKKFVYWLGTEPFLYIADP F+K+IS AVM KGW
Sbjct: 74 SFPSSKVSHDIHSTVFPHFAQWQASYGKKFVYWLGTEPFLYIADPHFMKQISVAVMAKGW 133
Query: 154 GKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKW 213
GKPAVFRNDR PMFGDGLVMTEGD W+RHRHILTPAFNPANLKAMTKFMVESTT+MLN+W
Sbjct: 134 GKPAVFRNDRRPMFGDGLVMTEGDVWVRHRHILTPAFNPANLKAMTKFMVESTTKMLNEW 193
Query: 214 SSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGV 273
SSLVNSA EIDVEREITATAGDIIA+ASFGI NGGGRDAF+ LRALQFTLFQNSTYVGV
Sbjct: 194 SSLVNSASGEIDVEREITATAGDIIAKASFGICNGGGRDAFDYLRALQFTLFQNSTYVGV 253
Query: 274 PFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGGG 333
PFS LF CP RTLAAKRLGA IDRLFLS++SERK S+ +SSSDLLSRLIE Q++ G
Sbjct: 254 PFSSLFFCPGRTLAAKRLGAAIDRLFLSLVSERKLSQ--NSSSDLLSRLIEGQKSTGDVG 313
Query: 334 KMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEIDF 393
KM LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEWQ+ILREEIKEVIGDKEIDF
Sbjct: 314 KMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDILREEIKEVIGDKEIDF 373
Query: 394 AMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDPTL 453
MLSGL+KMG VF+EVLRLYPSAPNIQRQAKGDI+VG +TIPKGTNIWID+V+MH DP L
Sbjct: 374 NMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIEVGSMTIPKGTNIWIDIVAMHRDPDL 433
Query: 454 WGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRFS 513
WG+D NEFKPERF +DSI GGCNHKMGYLPFGFGGRMCIG++LSSMEYKIVLTLILSRFS
Sbjct: 434 WGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKSLSSMEYKIVLTLILSRFS 493
Query: 514 LSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 546
LSLSP+YSHSPA LLSLRPAHGLPLIV PL P
Sbjct: 494 LSLSPNYSHSPATLLSLRPAHGLPLIVTPLHP 523
BLAST of Spg002423 vs. ExPASy TrEMBL
Match:
A0A6J1CIQ8 (cytokinin hydroxylase OS=Momordica charantia OX=3673 GN=LOC111011538 PE=3 SV=1)
HSP 1 Score: 875.9 bits (2262), Expect = 8.5e-251
Identity = 435/523 (83.17%), Postives = 475/523 (90.82%), Query Frame = 0
Query: 33 RVSIMVLFVKVVISWWILPILAHQKLKRNGFE-GPSPSFPLGNIAEMKKTMK------AA 92
RVS++VL V+VV SWWILP+LAH+KLKRNGF GP P FPLGNI E+K TMK AA
Sbjct: 13 RVSLVVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAAA 72
Query: 93 AAINGGSFA-SSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKI 152
AAINGG+ A SSS+N+SHDIHS VFP+FAQWQ SYGKKFVYWLGTEPFLYIA+PQFVK +
Sbjct: 73 AAINGGAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKNM 132
Query: 153 SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVE 212
SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMT+ MV
Sbjct: 133 SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRLMVG 192
Query: 213 STTQMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTL 272
STT+ML++W+ ++S PE+DVEREITATAGDIIA+ASFGI + GRDAFN LR LQFTL
Sbjct: 193 STTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQFTL 252
Query: 273 FQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIE 332
F+NS+YVGVPFS +FL P RTL AKRLG+EIDRLFLS+ISERKQS + +S+SDLLSRLIE
Sbjct: 253 FRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPD-ASASDLLSRLIE 312
Query: 333 SQRNGS--GGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEI 392
SQRN S GK LTTRELVDECKTFFFGGHETTALAISWT+LLLATRPEWQ ILREEI
Sbjct: 313 SQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQTILREEI 372
Query: 393 KEVIGDKEIDFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWI 452
KEVIGD+EIDFAMLS L+KMGWVFNEVLRLYPSAPNIQRQAKGDIKVGD TIPKGTNIWI
Sbjct: 373 KEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTIPKGTNIWI 432
Query: 453 DVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYK 512
D+V+MHHDP+LWG+DVNEFKPERFEKDSIHGGC HKMGYLPFGFGGRMCIGRNLSSMEYK
Sbjct: 433 DIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGRNLSSMEYK 492
Query: 513 IVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 546
IVLTLI+SRFSLS+SPSYSHSPAILLSLRPAHGLPLI+KPL P
Sbjct: 493 IVLTLIISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPLHP 534
BLAST of Spg002423 vs. ExPASy TrEMBL
Match:
A0A1S3AXX2 (cytokinin hydroxylase OS=Cucumis melo OX=3656 GN=LOC103484084 PE=3 SV=1)
HSP 1 Score: 852.4 bits (2201), Expect = 1.0e-243
Identity = 422/514 (82.10%), Postives = 458/514 (89.11%), Query Frame = 0
Query: 33 RVSIMVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAINGGS 92
RVSIMVLF KVVIS WILPILAH+KLKRNGFEGPSPSFPLGNI EMKK MKAAAA +
Sbjct: 16 RVSIMVLFGKVVISCWILPILAHRKLKRNGFEGPSPSFPLGNITEMKKIMKAAAAATAVT 75
Query: 93 FASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKG 152
SSSNN+SHDIHS +FPHFAQWQ SYGKKFVYWLGTEPFLYIADPQ VKKISEAV+GK
Sbjct: 76 NGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAVLGKS 135
Query: 153 WGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNK 212
WGKPAVFRNDREPMFGDGLVMTEGD+WIRHRHILTPAFNP NLKAMTKFMVESTT+ML +
Sbjct: 136 WGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKAMTKFMVESTTKMLEE 195
Query: 213 WSSLVNSAQPEIDVEREITATAGDIIAQASFGISN-GGGRDAFNSLRALQFTLFQNSTYV 272
W SLV S Q EI+VER+ITATAG+IIA+ASFGISN GGG +A N+LRALQ TLF+NS+YV
Sbjct: 196 WRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNNLRALQLTLFKNSSYV 255
Query: 273 GVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSG 332
GVPFS LFL PARTLAAKRLGA+IDRLFLS+IS RK S S SDLLSRLI S+ G
Sbjct: 256 GVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYSDLLSRLIGSEYTGGE 315
Query: 333 GGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEI 392
L+ RE+VDECKTFFFGGHETTALAISWTLLLLAT P+WQ ILREEIKEV+GDK+I
Sbjct: 316 A----LSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILREEIKEVVGDKDI 375
Query: 393 DFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDP 452
DF+MLS L+KMGWV+NEVLRLYPSAPNIQRQAKGDI +GD+ IPKGTNIWIDVV+MHHDP
Sbjct: 376 DFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNIWIDVVAMHHDP 435
Query: 453 TLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSR 512
TLWG+DVN+F P RF+ D+IHGGC HKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILS
Sbjct: 436 TLWGDDVNDFNPSRFQHDTIHGGCYHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILST 495
Query: 513 FSLSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 546
FSLSLSPSYSHSPA LLSLRPAHG+PLI+ PLQP
Sbjct: 496 FSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP 525
BLAST of Spg002423 vs. ExPASy TrEMBL
Match:
A0A5D3DI76 (Cytokinin hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G00640 PE=3 SV=1)
HSP 1 Score: 776.2 bits (2003), Expect = 9.2e-221
Identity = 382/469 (81.45%), Postives = 417/469 (88.91%), Query Frame = 0
Query: 78 MKKTMKAAAAINGGSFASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIAD 137
MKK MKAAAA + SSSNN+SHDIHS +FPHFAQWQ SYGKKFVYWLGTEPFLYIAD
Sbjct: 1 MKKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIAD 60
Query: 138 PQFVKKISEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKA 197
PQ VKKISEAV+GK WGKPAVFRNDREPMFGDGLVMTEGD+WIRHRHILTPAFNP NLKA
Sbjct: 61 PQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKA 120
Query: 198 MTKFMVESTTQMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISN-GGGRDAFNS 257
MTKFMVESTT+ML +W SLV S Q EI+VER+ITATAG+IIA+ASFGISN GGG +A N+
Sbjct: 121 MTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNN 180
Query: 258 LRALQFTLFQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSS 317
LRALQ TLF+NS+YVGVPFS LFL PARTLAAKRLGA+IDRLFLS+IS RK S S S
Sbjct: 181 LRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYS 240
Query: 318 DLLSRLIESQRNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQN 377
DLLSRLI S+ G L+ RE+VDECKTFFFGGHETTALAISWTLLLLAT P+WQ
Sbjct: 241 DLLSRLIGSEYTGGEA----LSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQR 300
Query: 378 ILREEIKEVIGDKEIDFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPK 437
ILREEIKEV+GDK+IDF+MLS L+KMGWV+NEVLRLYPSAPNIQRQAKGDI +GD+ IPK
Sbjct: 301 ILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPK 360
Query: 438 GTNIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNL 497
GTNIWIDVV+MHHDPTLWG+DVN+F P RF+ D+IHGGCNHKMGYLPFGFGGRMCIGRNL
Sbjct: 361 GTNIWIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNL 420
Query: 498 SSMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 546
SSMEYKIVLTLILS FSLSLSPSYSHSPA LLSLRPAHG+PLI+ PLQP
Sbjct: 421 SSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP 465
BLAST of Spg002423 vs. TAIR 10
Match:
AT5G52400.1 (cytochrome P450, family 715, subfamily A, polypeptide 1 )
HSP 1 Score: 634.8 bits (1636), Expect = 6.4e-182
Identity = 303/512 (59.18%), Postives = 385/512 (75.20%), Query Frame = 0
Query: 34 VSIMVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAINGGSF 93
V I ++F+K+ + WI P+ A +KL+ NGF GP+PSFP GN+ +MKK A+ ++
Sbjct: 19 VVICLMFLKLFLRCWIWPVRAQKKLRGNGFVGPAPSFPFGNLNDMKKLKMASVVVDN--- 78
Query: 94 ASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGW 153
+ SS ++HDIHSI PHFA+WQ YGK FVYWLG EPF+Y+ADP+F+ +S+ V+GK W
Sbjct: 79 SKSSTIINHDIHSIALPHFARWQQEYGKVFVYWLGIEPFVYVADPEFLSVMSKGVLGKSW 138
Query: 154 GKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKW 213
GKP VF+ DREPMFG GLVM EGDDW RHRHI+TPAF P NLK MT MVES + ML++W
Sbjct: 139 GKPNVFKKDREPMFGTGLVMVEGDDWTRHRHIITPAFAPLNLKVMTNMMVESVSNMLDRW 198
Query: 214 SSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGV 273
+NS PE D+E EI TAG+IIA+ SFG++ G +LRA+QF LF ++ YVGV
Sbjct: 199 GIQINSGNPEFDMESEIIGTAGEIIAKTSFGVTGENGTQVLKNLRAVQFALFNSNRYVGV 258
Query: 274 PFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQS--EENSSSSDLLSRLIESQRNGSG 333
PFS + L +T+ AK LG EID L LS I++RK S E + DLL L+++ + G+
Sbjct: 259 PFSNI-LSYKQTVKAKGLGHEIDGLLLSFINKRKISLAEGDDQGHDLLGMLLKADQKGN- 318
Query: 334 GGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEI 393
T +ELVDECKTFFF GHETTALA++WT +LLA PEWQ+ +REEI+EVIGD +I
Sbjct: 319 -----FTAKELVDECKTFFFAGHETTALALTWTFMLLAIHPEWQDTIREEIREVIGDSKI 378
Query: 394 DFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDP 453
++ L+GL+KM WV NEVLRLYP APN QRQA+ DI+V IP GTNIWIDVV+MHHD
Sbjct: 379 EYNKLAGLKKMSWVMNEVLRLYPPAPNAQRQARNDIEVNGRVIPNGTNIWIDVVAMHHDV 438
Query: 454 TLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSR 513
LWG+DVNEFKPERF+ ++HGGC +KMGY+PFGFGGRMCIGRNL++MEYKIVL+L+LSR
Sbjct: 439 ELWGDDVNEFKPERFD-GNLHGGCKNKMGYMPFGFGGRMCIGRNLTTMEYKIVLSLVLSR 498
Query: 514 FSLSLSPSYSHSPAILLSLRPAHGLPLIVKPL 544
F +S+SP Y HSP +LSLRP +GLPLI++PL
Sbjct: 499 FEISVSPGYRHSPTYMLSLRPGYGLPLIIRPL 519
BLAST of Spg002423 vs. TAIR 10
Match:
AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )
HSP 1 Score: 330.1 bits (845), Expect = 3.4e-90
Identity = 185/518 (35.71%), Postives = 283/518 (54.63%), Query Frame = 0
Query: 34 VSIMVLFVKV----VISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAIN 93
V +M L ++V + +++ P + ++R G GP P GNI ++ K + +A
Sbjct: 12 VIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDISKMLSHSA--- 71
Query: 94 GGSFASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKI---SE 153
++ +++ H+I + PH+ W YGK+F+ W GTEP L + + + +K++
Sbjct: 72 ----SNDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKELLTKHN 131
Query: 154 AVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVEST 213
V GK W + + + G GL+M G+ W RH+ PAF LK K MVE T
Sbjct: 132 PVTGKSW----LQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVECT 191
Query: 214 TQMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQ 273
M + L E+++ E+ DII++ FG S G++ F+ L LQ Q
Sbjct: 192 KMMAER---LRKEVGEEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQ 251
Query: 274 NSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQ 333
+ ++ P S FL K L E++RL + +I RK S E SS L+
Sbjct: 252 ATRHLCFPGS-RFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLL 311
Query: 334 RNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVI 393
N K L + ++DECKTFFF GHETT+L ++WTL+LLA P WQ+ +R+E+++V
Sbjct: 312 LNQMDSNKNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQVC 371
Query: 394 GDKEI-DFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVV 453
G + LS L + V NE LRLYP A + R A DIK+GD+ IPKG +IWI V+
Sbjct: 372 GQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIPVL 431
Query: 454 SMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVL 513
++HH LWGED NEF PERF S H ++PF G R CIG+ + ME KI+L
Sbjct: 432 AIHHSNELWGEDANEFNPERFTTRSF-ASSRH---FMPFAAGPRNCIGQTFAMMEAKIIL 491
Query: 514 TLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPL 544
+++S+FS ++S +Y H+P ++L+++P +G+ L++KPL
Sbjct: 492 AMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPL 510
BLAST of Spg002423 vs. TAIR 10
Match:
AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )
HSP 1 Score: 327.4 bits (838), Expect = 2.2e-89
Identity = 184/527 (34.91%), Postives = 284/527 (53.89%), Query Frame = 0
Query: 35 SIMVLFVKVVI--------SWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAA 94
S++V+FV ++ +W+ P + +++ G GP P GNI E+ +
Sbjct: 8 SLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEI-------S 67
Query: 95 AINGGSFASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKI-- 154
A+ S + +++ HDI + PH+ W YGK+F+ W GT+P L + + + +K++
Sbjct: 68 AMVSQSASKDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLM 127
Query: 155 -SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMV 214
V G+ W + +N G GL+M G DW RH+ PAF LK + MV
Sbjct: 128 KHNGVSGRSWLQQQGTKN----FIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMV 187
Query: 215 ESTTQMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFT 274
E T++++ + V E+++ E+ DII++ FG S G++ FN L LQ
Sbjct: 188 ECTSKLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRR 247
Query: 275 LFQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSS----DLL 334
Q + ++ P S FL K L E++RL + +I R+ E SS DLL
Sbjct: 248 CAQATRHLCFPGS-RFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLL 307
Query: 335 SRLIESQRNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILR 394
L+ + ++DECKTFFF GHETTAL ++WT +LLA P WQ +R
Sbjct: 308 GLLLNEMDIDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVR 367
Query: 395 EEIKEVIGDKEI-DFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGT 454
EE++EV G + LS L + V NE LRLYP A + R A D+K+GD+TIPKG
Sbjct: 368 EEVREVFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGL 427
Query: 455 NIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMG--YLPFGFGGRMCIGRNL 514
+IWI V+++HH LWG+D N+F PERF GG G ++PF G R CIG+
Sbjct: 428 SIWIPVLAIHHSEELWGKDANQFNPERF------GGRPFASGRHFIPFAAGPRNCIGQQF 487
Query: 515 SSMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPL 544
+ ME KI+L ++S+F+ ++S +Y H+P ++L+++P +G+ +I+KPL
Sbjct: 488 ALMEAKIILATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516
BLAST of Spg002423 vs. TAIR 10
Match:
AT5G24900.1 (cytochrome P450, family 714, subfamily A, polypeptide 2 )
HSP 1 Score: 306.6 bits (784), Expect = 4.0e-83
Identity = 172/515 (33.40%), Postives = 273/515 (53.01%), Query Frame = 0
Query: 39 LFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAINGGSFASSSN 98
++ + V+ W + + LK G +GP PS GN++EM++ A S N
Sbjct: 27 VYGRAVVEQWRM----RRSLKLQGVKGPPPSIFNGNVSEMQRIQSEA------KHCSGDN 86
Query: 99 NVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGWGKPAV 158
+SHD S +FPHF W+ YG+ + Y G + LYI P+ VK++S+ G+
Sbjct: 87 IISHDYSSSLFPHFDHWRKQYGRIYTYSTGLKQHLYINHPEMVKELSQ-TNTLNLGRITH 146
Query: 159 FRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKWSSLVN 218
P+ G+G++ + G W R I+ F +K M MVES MLNKW +V
Sbjct: 147 ITKRLNPILGNGIITSNGPHWAHQRRIIAYEFTHDKIKGMVGLMVESAMPMLNKWEEMVK 206
Query: 219 SAQP---EIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGVPF 278
+I V+ ++ + D+IA+A FG S G+ F+ +R L + + S V F
Sbjct: 207 RGGEMGCDIRVDEDLKDVSADVIAKACFGSSFSKGKAIFSMIRDLLTAITKRS--VLFRF 266
Query: 279 SGLFLCPARTLAAKRLG-AEIDRLFLSV-------ISERKQSEENSSSSDLLSRLIESQR 338
+G +K+ G +ID L + + + ER+ +++ DL+ ++E
Sbjct: 267 NGF---TDMVFGSKKHGDVDIDALEMELESSIWETVKEREIECKDTHKKDLMQLILEGAM 326
Query: 339 NGSGGGKMRLTT--RELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEV 398
G + R +VD CK+ +F GH++TA+++SW L+LLA P WQ +R+EI
Sbjct: 327 RSCDGNLWDKSAYRRFVVDNCKSIYFAGHDSTAVSVSWCLMLLALNPSWQVKIRDEILSS 386
Query: 399 IGDKEIDFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVV 458
+ D + L+ + V E +RLYP AP + R+A DI++GD+ +PKG IW +
Sbjct: 387 CKNGIPDAESIPNLKTVTMVIQETMRLYPPAPIVGREASKDIRLGDLVVPKGVCIWTLIP 446
Query: 459 SMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVL 518
++H DP +WG D N+FKPERF + I C + Y+PFG G R C+G+N ME K+++
Sbjct: 447 ALHRDPEIWGPDANDFKPERF-SEGISKACKYPQSYIPFGLGPRTCVGKNFGMMEVKVLV 506
Query: 519 TLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIV 541
+LI+S+FS +LSP+Y HSP+ L + P HG+ + V
Sbjct: 507 SLIVSKFSFTLSPTYQHSPSHKLLVEPQHGVVIRV 524
BLAST of Spg002423 vs. TAIR 10
Match:
AT5G24910.1 (cytochrome P450, family 714, subfamily A, polypeptide 1 )
HSP 1 Score: 305.1 bits (780), Expect = 1.2e-82
Identity = 167/511 (32.68%), Postives = 272/511 (53.23%), Query Frame = 0
Query: 39 LFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKKTMKAAAAINGGSFASSSN 98
++ KV+ W + +KL G +GP PS GN+ EM+K + ++ S N
Sbjct: 27 VYGKVMAEQWRM----RRKLTMQGVKGPPPSLFRGNVPEMQKIQ--SQIMSNSKHYSGDN 86
Query: 99 NVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGWGKPAV 158
++HD S +FP+ W+ YG+ + Y G + LY+ P+ VK++++A GK +
Sbjct: 87 IIAHDYTSSLFPYLDHWRKQYGRVYTYSTGVKQHLYMNHPELVKELNQA-NTLNLGKVSY 146
Query: 159 FRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKWSSLVN 218
+ + G G++ + G W R I+ P F +K M +VES ML+KW ++
Sbjct: 147 VTKRLKSILGRGVITSNGPHWAHQRRIIAPEFFLDKVKGMVGLVVESAMPMLSKWEEMMK 206
Query: 219 SAQP---EIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGV-P 278
+I V+ ++ A + D+I++A FG S G++ F+ LR LQ + N+ +
Sbjct: 207 REGEMVCDIIVDEDLRAASADVISRACFGSSFSKGKEIFSKLRCLQKAITHNNILFSLNG 266
Query: 279 FSGLFLCPARTLAAK--RLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGG 338
F+ + + K L I+ L + ER++ DL+ ++E R+ G
Sbjct: 267 FTDVVFGTKKHGNGKIDELERHIESLIWETVKERERECVGDHKKDLMQLILEGARSSCDG 326
Query: 339 G---KMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDK 398
K + +VD CK+ +F GHET+A+A+SW L+LLA P WQ +R+E+ +
Sbjct: 327 NLEDKTQSYKSFVVDNCKSIYFAGHETSAVAVSWCLMLLALNPSWQTRIRDEVFLHCKNG 386
Query: 399 EIDFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHH 458
D +S L+ + V E LRLYP A + R+A D K+G++ +PKG IW + ++H
Sbjct: 387 IPDADSISNLKTVTMVIQETLRLYPPAAFVSREALEDTKLGNLVVPKGVCIWTLIPTLHR 446
Query: 459 DPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLIL 518
DP +WG D NEF PERF + + C H ++PFG G R+C+G+N ME K++++LI+
Sbjct: 447 DPEIWGADANEFNPERF-SEGVSKACKHPQSFVPFGLGTRLCLGKNFGMMELKVLVSLIV 506
Query: 519 SRFSLSLSPSYSHSPAILLSLRPAHGLPLIV 541
SRFS +LSP+Y HSP + + P HG+ + V
Sbjct: 507 SRFSFTLSPTYQHSPVFRMLVEPQHGVVIRV 529
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q9ZW95 | 4.7e-89 | 35.71 | Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1 | [more] |
Q2QYH7 | 8.1e-89 | 35.62 | Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 ... | [more] |
Q9FF18 | 3.1e-88 | 34.91 | Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1 | [more] |
B6SSW8 | 3.9e-83 | 34.92 | Cytochrome P450 714B3 OS=Zea mays OX=4577 GN=CYP714B3 PE=2 SV=1 | [more] |
Q7XHW5 | 4.3e-82 | 33.27 | Cytochrome P450 714B1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714B1 PE=1 ... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1G224 | 4.8e-254 | 84.57 | cytochrome P450 714C2-like OS=Cucurbita moschata OX=3662 GN=LOC111449972 PE=3 SV... | [more] |
A0A6J1HYD5 | 2.0e-252 | 84.77 | cytochrome P450 714C2-like OS=Cucurbita maxima OX=3661 GN=LOC111467387 PE=3 SV=1 | [more] |
A0A6J1CIQ8 | 8.5e-251 | 83.17 | cytokinin hydroxylase OS=Momordica charantia OX=3673 GN=LOC111011538 PE=3 SV=1 | [more] |
A0A1S3AXX2 | 1.0e-243 | 82.10 | cytokinin hydroxylase OS=Cucumis melo OX=3656 GN=LOC103484084 PE=3 SV=1 | [more] |
A0A5D3DI76 | 9.2e-221 | 81.45 | Cytokinin hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold38... | [more] |
Match Name | E-value | Identity | Description | |
AT5G52400.1 | 6.4e-182 | 59.18 | cytochrome P450, family 715, subfamily A, polypeptide 1 | [more] |
AT1G67110.1 | 3.4e-90 | 35.71 | cytochrome P450, family 735, subfamily A, polypeptide 2 | [more] |
AT5G38450.1 | 2.2e-89 | 34.91 | cytochrome P450, family 735, subfamily A, polypeptide 1 | [more] |
AT5G24900.1 | 4.0e-83 | 33.40 | cytochrome P450, family 714, subfamily A, polypeptide 2 | [more] |
AT5G24910.1 | 1.2e-82 | 32.68 | cytochrome P450, family 714, subfamily A, polypeptide 1 | [more] |