Spg002265 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg002265 vs. NCBI nr
Match: XP_038883313.1 (aspartic proteinase nepenthesin-1 [Benincasa hispida])

HSP 1 Score: 772.7 bits (1994), Expect = 1.8e-219
Identity = 393/459 (85.62%), Postives = 420/459 (91.50%), Query Frame = 0

Query: 1   MTNSLRSFRYPIAMV-VLVTTLFTGTSSALSSTRSRRALQQP-ESPSDGFRVRLNHVDHV 60
           MT SL S+R  I +V VL+TTLF  T +      SRRALQ+  E PS+GF+V+LNHVDHV
Sbjct: 1   MTVSLHSYRCLILLVIVLITTLFIDTLAL-----SRRALQKSNELPSNGFKVKLNHVDHV 60

Query: 61  KNLTRFERLQRGVMRGRNRLQRLNAMVLAANAAVGDQVQAPVVAGNGEFLMKLAIGSPPR 120
           KNLTRFERL+RGV RG+NRL RLNAMVLAANAAVG+QVQAPVVAGNGEFLMKLAIG+PP+
Sbjct: 61  KNLTRFERLRRGVARGKNRLHRLNAMVLAANAAVGEQVQAPVVAGNGEFLMKLAIGTPPK 120

Query: 121 SFSAIMDTGSDLIWTQCKPCQQCFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTCDGD 180
           SFSAIMDTGSDLIWTQCKPCQQCFDQ+TPIFDP+ SSSFSK+SCSSELCG LPTSTC  D
Sbjct: 121 SFSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKASSSFSKISCSSELCGPLPTSTCSSD 180

Query: 181 SCEYFYTYGDFSSTHGVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGLVGL 240
            CEY YTYGD SST GVLA ETFTFGDSSDDQVSI+G+GFGCGDDNEGDGFSQGAGLVGL
Sbjct: 181 GCEYLYTYGDSSSTQGVLALETFTFGDSSDDQVSITGLGFGCGDDNEGDGFSQGAGLVGL 240

Query: 241 GRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANVKPKTSQEEMKTTPLIRNPSQP 300
           GRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLAN+KPKT+++EMKTTPLIRNPSQP
Sbjct: 241 GRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANIKPKTTKDEMKTTPLIRNPSQP 300

Query: 301 SFYYLSLQGISVGGTQLSMPKATFELRDDGSGGLIIDSGTTITYIEKSAFNLLKKEFIAQ 360
           SFYYLSLQGISVG TQLS+PK TFEL DDGSGG+IIDSGTTITYIE +AF LLK EFIAQ
Sbjct: 301 SFYYLSLQGISVGSTQLSIPKTTFELHDDGSGGVIIDSGTTITYIENTAFTLLKNEFIAQ 360

Query: 361 MKLPVHDSGTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLA 420
           M LPV DSGTGGLDLCFNLPAEATQVEVPKLTFHF+GADLELPGENYMIGDSR GLICLA
Sbjct: 361 MNLPVDDSGTGGLDLCFNLPAEATQVEVPKLTFHFEGADLELPGENYMIGDSRTGLICLA 420

Query: 421 IGSSSGMSIFGNLQQQNFMVVHDLQEETISFLPTQCDSI 458
           IGSS GMSIFGNLQQQNFMVVHDLQEET+SFLPTQCDSI
Sbjct: 421 IGSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 454

BLAST of Spg002265 vs. NCBI nr
Match: XP_023543561.1 (aspartic proteinase nepenthesin-1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 772.3 bits (1993), Expect = 2.3e-219
Identity = 387/451 (85.81%), Postives = 411/451 (91.13%), Query Frame = 0

Query: 7   SFRYPIAMVVLVTTLFTGTSSALSSTRSRRALQQPESPSDGFRVRLNHVDHVKNLTRFER 66
           S RY I  VVL  T+    +SA SS+ SRRAL+QP+ PSDGFRV LNHVDHVKNLTRFE+
Sbjct: 4   SLRYLIVSVVLSITMLFIHTSASSSSHSRRALRQPKLPSDGFRVSLNHVDHVKNLTRFEQ 63

Query: 67  LQRGVMRGRNRLQRLNAMVLAANAAVGDQVQAPVVAGNGEFLMKLAIGSPPRSFSAIMDT 126
           LQRGV RG+ RL RLNAM+LAAN  VG +VQAPVVAGNGEFLMKLAIGSPPRSFSAIMDT
Sbjct: 64  LQRGVARGKTRLHRLNAMMLAANVGVGGRVQAPVVAGNGEFLMKLAIGSPPRSFSAIMDT 123

Query: 127 GSDLIWTQCKPCQQCFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTCDGDSCEYFYTY 186
           GSDLIWTQCKPCQQCFDQ+TPIFDP+ESSSFSK+SCSSELC ALPTSTC  D CEYFYTY
Sbjct: 124 GSDLIWTQCKPCQQCFDQATPIFDPKESSSFSKISCSSELCDALPTSTCSSDECEYFYTY 183

Query: 187 GDFSSTHGVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGLVGLGRGPLSLV 246
           GD+SSTHGVLAAETFTFGDSS DQVSI G+GFGCGDDNEGDGFSQG GLVGLGRGPLSLV
Sbjct: 184 GDYSSTHGVLAAETFTFGDSSQDQVSIPGLGFGCGDDNEGDGFSQGEGLVGLGRGPLSLV 243

Query: 247 SQLKEQKFAYCLTAIDDSKPSSLLLGSLANVKPKTSQEEMKTTPLIRNPSQPSFYYLSLQ 306
           SQLKEQKF+YCLTAIDD+KPSSLLLGSLANVKPK S+ E+KTTPLIRNPSQPSFYYLSLQ
Sbjct: 244 SQLKEQKFSYCLTAIDDTKPSSLLLGSLANVKPKASEGEIKTTPLIRNPSQPSFYYLSLQ 303

Query: 307 GISVGGTQLSMPKATFELRDDGSGGLIIDSGTTITYIEKSAFNLLKKEFIAQMKLPVHDS 366
           GISVGGTQL +PKATFEL DDGSGG+IIDSGTTITYIEK+AF LLKKEF++QMKLPV DS
Sbjct: 304 GISVGGTQLPIPKATFELHDDGSGGVIIDSGTTITYIEKNAFTLLKKEFVSQMKLPVDDS 363

Query: 367 GTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSSGMS 426
           GT GLDLCFNLP E TQVEVPKLTFHFKGADLELPGENYMIGDSRA LICLAIGSSSGMS
Sbjct: 364 GTSGLDLCFNLPPETTQVEVPKLTFHFKGADLELPGENYMIGDSRAELICLAIGSSSGMS 423

Query: 427 IFGNLQQQNFMVVHDLQEETISFLPTQCDSI 458
           IFGNLQQQN MVVHDLQEET+SFLPTQC  I
Sbjct: 424 IFGNLQQQNIMVVHDLQEETVSFLPTQCSDI 454

BLAST of Spg002265 vs. NCBI nr
Match: XP_022928703.1 (aspartic proteinase nepenthesin-1 [Cucurbita moschata])

HSP 1 Score: 770.8 bits (1989), Expect = 6.7e-219
Identity = 387/451 (85.81%), Postives = 410/451 (90.91%), Query Frame = 0

Query: 7   SFRYPIAMVVLVTTLFTGTSSALSSTRSRRALQQPESPSDGFRVRLNHVDHVKNLTRFER 66
           S RY I  VVL  T+    +SA SS+ SRRAL QP+ PSDGFRV LNHVDHVKNLTRFER
Sbjct: 4   SLRYLIISVVLSITMLFIHTSASSSSLSRRALWQPKLPSDGFRVSLNHVDHVKNLTRFER 63

Query: 67  LQRGVMRGRNRLQRLNAMVLAANAAVGDQVQAPVVAGNGEFLMKLAIGSPPRSFSAIMDT 126
           LQRGV RG+ RL RLNAMVLAAN  VG +VQAPVVAGNGEFLMKLAIGSPPRSFSAIMDT
Sbjct: 64  LQRGVARGKTRLHRLNAMVLAANVGVGGRVQAPVVAGNGEFLMKLAIGSPPRSFSAIMDT 123

Query: 127 GSDLIWTQCKPCQQCFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTCDGDSCEYFYTY 186
           GSDLIWTQCKPCQQCFDQ+TPIFDP+ESSSFSK+SCSSELC ALPTSTC  D CEYFYTY
Sbjct: 124 GSDLIWTQCKPCQQCFDQATPIFDPKESSSFSKISCSSELCDALPTSTCSSDECEYFYTY 183

Query: 187 GDFSSTHGVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGLVGLGRGPLSLV 246
           GD+SSTHGVLAAETFTFGDSS DQVSI G+GFGCGDDNEGDGFSQG GLVGLGRGPLSLV
Sbjct: 184 GDYSSTHGVLAAETFTFGDSSQDQVSIPGLGFGCGDDNEGDGFSQGEGLVGLGRGPLSLV 243

Query: 247 SQLKEQKFAYCLTAIDDSKPSSLLLGSLANVKPKTSQEEMKTTPLIRNPSQPSFYYLSLQ 306
           SQLKEQKF+YCLTAIDD+KPSSLL+GSLANVKPK S+ E+KTTPLIRNPSQPSFYYLSLQ
Sbjct: 244 SQLKEQKFSYCLTAIDDTKPSSLLMGSLANVKPKASEGEIKTTPLIRNPSQPSFYYLSLQ 303

Query: 307 GISVGGTQLSMPKATFELRDDGSGGLIIDSGTTITYIEKSAFNLLKKEFIAQMKLPVHDS 366
           GISVGGTQL +PKATFEL DDGSGG+IIDSGTTITYIEK+AF LLKKEF++QMKLPV DS
Sbjct: 304 GISVGGTQLPIPKATFELHDDGSGGVIIDSGTTITYIEKNAFTLLKKEFVSQMKLPVDDS 363

Query: 367 GTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSSGMS 426
           GT GLDLCFNLP E TQVEVPKLTFHFKGADLELPGENYMIGDS+A LICLAIGSSSGMS
Sbjct: 364 GTSGLDLCFNLPPETTQVEVPKLTFHFKGADLELPGENYMIGDSKAELICLAIGSSSGMS 423

Query: 427 IFGNLQQQNFMVVHDLQEETISFLPTQCDSI 458
           IFGNLQQQN MVVHDLQEET+SFLPTQC  I
Sbjct: 424 IFGNLQQQNIMVVHDLQEETVSFLPTQCSEI 454

BLAST of Spg002265 vs. NCBI nr
Match: XP_022154910.1 (aspartic proteinase nepenthesin-1 [Momordica charantia])

HSP 1 Score: 769.6 bits (1986), Expect = 1.5e-218
Identity = 390/460 (84.78%), Postives = 419/460 (91.09%), Query Frame = 0

Query: 1   MTNSLRSFRYPIAMVVLVTTLFTGTSSALSSTRSRRALQQPESPSDGFRVRLNHVD-HVK 60
           M +SL S RYPI +V ++ TLF    +  SST SRRALQQ +  ++GFR+RL+HVD HVK
Sbjct: 1   MLDSLCSVRYPIVLVAVLATLFFIDLTVSSSTLSRRALQQQKLLNNGFRMRLHHVDHHVK 60

Query: 61  NLTRFERLQRGVMRGRNRLQRLNAMVLAA--NAAVGDQVQAPVVAGNGEFLMKLAIGSPP 120
           NLTRFERLQRG  RGRNRLQRLNAMVLAA   AAVGDQVQAPVVAGNGEFLMKLAIGSPP
Sbjct: 61  NLTRFERLQRGAARGRNRLQRLNAMVLAAAGGAAVGDQVQAPVVAGNGEFLMKLAIGSPP 120

Query: 121 RSFSAIMDTGSDLIWTQCKPCQQCFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTCDG 180
           +SFSAIMDTGSDLIWTQCKPCQQCFDQSTPIFDP+ESSSFSKLSCSSELCGALPTS C  
Sbjct: 121 KSFSAIMDTGSDLIWTQCKPCQQCFDQSTPIFDPKESSSFSKLSCSSELCGALPTSACSN 180

Query: 181 DSCEYFYTYGDFSSTHGVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGLVG 240
           D CEY YTYGD+SSTHG+L AETFTFGD  +DQVSIS +GFGCGDDNEGDGFSQGAGLVG
Sbjct: 181 DGCEYLYTYGDYSSTHGLLGAETFTFGDPGEDQVSISEIGFGCGDDNEGDGFSQGAGLVG 240

Query: 241 LGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANVKPKTSQEEMKTTPLIRNPSQ 300
           LGRGPLSLVSQLKEQKFAYCLT IDDSKPSSLL+GSLANVKPK SQ+E+KTTPLIRNPSQ
Sbjct: 241 LGRGPLSLVSQLKEQKFAYCLTPIDDSKPSSLLMGSLANVKPKKSQDEIKTTPLIRNPSQ 300

Query: 301 PSFYYLSLQGISVGGTQLSMPKATFELRDDGSGGLIIDSGTTITYIEKSAFNLLKKEFIA 360
           PSFYYLSL+GISVGG+ L++P+ TFELRDDGSGG+IIDSGTTITYI+KSAF LLKKEFIA
Sbjct: 301 PSFYYLSLEGISVGGSHLAIPQPTFELRDDGSGGVIIDSGTTITYIDKSAFTLLKKEFIA 360

Query: 361 QMKLPVHDSGTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICL 420
           QMKLPV DSGTGGLDLCF LP+EATQVEVPKLTFHFK ADLELPGENYMIGDS AGL+CL
Sbjct: 361 QMKLPVDDSGTGGLDLCFKLPSEATQVEVPKLTFHFKDADLELPGENYMIGDSAAGLLCL 420

Query: 421 AIGSSSGMSIFGNLQQQNFMVVHDLQEETISFLPTQCDSI 458
           AIGSSSGMSIFGNLQQQNFMVVHDLQEETISF+PTQCD I
Sbjct: 421 AIGSSSGMSIFGNLQQQNFMVVHDLQEETISFVPTQCDGI 460

BLAST of Spg002265 vs. NCBI nr
Match: KAG7033562.1 (Aspartic proteinase nepenthesin-1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 769.2 bits (1985), Expect = 2.0e-218
Identity = 387/451 (85.81%), Postives = 409/451 (90.69%), Query Frame = 0

Query: 7   SFRYPIAMVVLVTTLFTGTSSALSSTRSRRALQQPESPSDGFRVRLNHVDHVKNLTRFER 66
           S RY I  VVL  T+    +SA SS+ SRRAL QP+ PSDGFRV LNHVDHVKNLTRFER
Sbjct: 4   SLRYLIISVVLSITMLFIHTSASSSSLSRRALWQPKLPSDGFRVSLNHVDHVKNLTRFER 63

Query: 67  LQRGVMRGRNRLQRLNAMVLAANAAVGDQVQAPVVAGNGEFLMKLAIGSPPRSFSAIMDT 126
           LQRGV RG+ RL RLNAM+LAAN  VG +VQAPVVAGNGEFLMKLAIGSPPRSFSAIMDT
Sbjct: 64  LQRGVARGKTRLHRLNAMMLAANVGVGGRVQAPVVAGNGEFLMKLAIGSPPRSFSAIMDT 123

Query: 127 GSDLIWTQCKPCQQCFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTCDGDSCEYFYTY 186
           GSDLIWTQCKPCQQCFDQ+TPIFDP+ESSSFSK+SCSSELC ALPTSTC  D CEYFYTY
Sbjct: 124 GSDLIWTQCKPCQQCFDQATPIFDPKESSSFSKISCSSELCDALPTSTCSSDECEYFYTY 183

Query: 187 GDFSSTHGVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGLVGLGRGPLSLV 246
           GD+SSTHGVLAAETFTFGDSS DQVSI G+GFGCGDDNEGDGFSQG GLVGLGRGPLSLV
Sbjct: 184 GDYSSTHGVLAAETFTFGDSSQDQVSIPGLGFGCGDDNEGDGFSQGEGLVGLGRGPLSLV 243

Query: 247 SQLKEQKFAYCLTAIDDSKPSSLLLGSLANVKPKTSQEEMKTTPLIRNPSQPSFYYLSLQ 306
           SQLKEQKF+YCLTAIDD+KPSSLLLGSLANVKPK S+ E+KTTPLI NPSQPSFYYLSLQ
Sbjct: 244 SQLKEQKFSYCLTAIDDTKPSSLLLGSLANVKPKASEGEIKTTPLISNPSQPSFYYLSLQ 303

Query: 307 GISVGGTQLSMPKATFELRDDGSGGLIIDSGTTITYIEKSAFNLLKKEFIAQMKLPVHDS 366
           GISVGGTQL +PKATFEL DDGSGG+IIDSGTTITYIEK+AF LLKKEF++QMKLPV DS
Sbjct: 304 GISVGGTQLPIPKATFELHDDGSGGVIIDSGTTITYIEKNAFTLLKKEFVSQMKLPVDDS 363

Query: 367 GTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSSGMS 426
           GT GLDLCFNLP E TQVEVPKLTFHFKGADLELPGENYMIGDSRA LICLAIGSSSGMS
Sbjct: 364 GTSGLDLCFNLPPETTQVEVPKLTFHFKGADLELPGENYMIGDSRAELICLAIGSSSGMS 423

Query: 427 IFGNLQQQNFMVVHDLQEETISFLPTQCDSI 458
           IFGNLQQQN MVVHDLQEET+SFLPTQC  I
Sbjct: 424 IFGNLQQQNIMVVHDLQEETVSFLPTQCSDI 454

BLAST of Spg002265 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 6.9e-118
Identity = 233/457 (50.98%), Postives = 306/457 (66.96%), Query Frame = 0

Query: 1   MTNSLRSFRYPIAMVVLVTTLFTGTSSALSSTRSRRAL-QQPESPSDGFRVRLNHVDHVK 60
           M +SL SF   +++V +          A + + SR AL  + E+   GF++ L HVD  K
Sbjct: 1   MASSLYSFLLALSIVYIFV--------APTHSTSRTALNHRHEAKVTGFQIMLEHVDSGK 60

Query: 61  NLTRFERLQRGVMRGRNRLQRLNAMVLAANAAVGDQVQAPVVAGNGEFLMKLAIGSPPRS 120
           NLT+F+ L+R + RG  RLQRL AM+   N   G  V+  V AG+GE+LM L+IG+P + 
Sbjct: 61  NLTKFQLLERAIERGSRRLQRLEAML---NGPSG--VETSVYAGDGEYLMNLSIGTPAQP 120

Query: 121 FSAIMDTGSDLIWTQCKPCQQCFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTCDGDS 180
           FSAIMDTGSDLIWTQC+PC QCF+QSTPIF+P+ SSSFS L CSS+LC AL + TC  + 
Sbjct: 121 FSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQLCQALSSPTCSNNF 180

Query: 181 CEYFYTYGDFSSTHGVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGLVGLG 240
           C+Y Y YGD S T G +  ET TFG      VSI  + FGCG++N+G G   GAGLVG+G
Sbjct: 181 CQYTYGYGDGSETQGSMGTETLTFG-----SVSIPNITFGCGENNQGFGQGNGAGLVGMG 240

Query: 241 RGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANVKPKTSQEEMKTTPLIRNPSQPS 300
           RGPLSL SQL   KF+YC+T I  S PS+LLLGSLAN     S      T LI++   P+
Sbjct: 241 RGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGS----PNTTLIQSSQIPT 300

Query: 301 FYYLSLQGISVGGTQLSMPKATFELR-DDGSGGLIIDSGTTITYIEKSAFNLLKKEFIAQ 360
           FYY++L G+SVG T+L +  + F L  ++G+GG+IIDSGTT+TY   +A+  +++EFI+Q
Sbjct: 301 FYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQ 360

Query: 361 MKLPVHDSGTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLA 420
           + LPV +  + G DLCF  P++ + +++P    HF G DLELP ENY I  S  GLICLA
Sbjct: 361 INLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGGDLELPSENYFISPSN-GLICLA 420

Query: 421 IGSSS-GMSIFGNLQQQNFMVVHDLQEETISFLPTQC 455
           +GSSS GMSIFGN+QQQN +VV+D     +SF   QC
Sbjct: 421 MGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

BLAST of Spg002265 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 2.7e-114
Identity = 214/447 (47.87%), Postives = 300/447 (67.11%), Query Frame = 0

Query: 11  PIAMVVLVTTLFTGTSSALSSTRSRRALQQPES-PSDGFRVRLNHVDHVKNLTRFERLQR 70
           P+  VVL   + +   +  SST     L   +  P  G RV L  VD  KNLT++E ++R
Sbjct: 4   PLYSVVLGLAIVSAIVAPTSSTSRGTLLHHGQKRPQPGLRVDLEQVDSGKNLTKYELIKR 63

Query: 71  GVMRGRNRLQRLNAMVLAANAAVGDQVQAPVVAGNGEFLMKLAIGSPPRSFSAIMDTGSD 130
            + RG  R++ +NAM+ +++      ++ PV AG+GE+LM +AIG+P  SFSAIMDTGSD
Sbjct: 64  AIKRGERRMRSINAMLQSSSG-----IETPVYAGDGEYLMNVAIGTPDSSFSAIMDTGSD 123

Query: 131 LIWTQCKPCQQCFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTCDGDSCEYFYTYGDF 190
           LIWTQC+PC QCF Q TPIF+P++SSSFS L C S+ C  LP+ TC+ + C+Y Y YGD 
Sbjct: 124 LIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQDLPSETCNNNECQYTYGYGDG 183

Query: 191 SSTHGVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGLVGLGRGPLSLVSQL 250
           S+T G +A ETFTF  S     S+  + FGCG+DN+G G   GAGL+G+G GPLSL SQL
Sbjct: 184 STTQGYMATETFTFETS-----SVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQL 243

Query: 251 KEQKFAYCLTAIDDSKPSSLLLGSLANVKPKTSQEEMKTTPLIRNPSQPSFYYLSLQGIS 310
              +F+YC+T+   S PS+L LGS A+  P+ S     +T LI +   P++YY++LQGI+
Sbjct: 244 GVGQFSYCMTSYGSSSPSTLALGSAASGVPEGS----PSTTLIHSSLNPTYYYITLQGIT 303

Query: 311 VGGTQLSMPKATFELRDDGSGGLIIDSGTTITYIEKSAFNLLKKEFIAQMKLPVHDSGTG 370
           VGG  L +P +TF+L+DDG+GG+IIDSGTT+TY+ + A+N + + F  Q+ LP  D  + 
Sbjct: 304 VGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVDESSS 363

Query: 371 GLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSS--GMSI 430
           GL  CF  P++ + V+VP+++  F G  L L  +N +I  +  G+ICLA+GSSS  G+SI
Sbjct: 364 GLSTCFQQPSDGSTVQVPEISMQFDGGVLNLGEQNILISPAE-GVICLAMGSSSQLGISI 423

Query: 431 FGNLQQQNFMVVHDLQEETISFLPTQC 455
           FGN+QQQ   V++DLQ   +SF+PTQC
Sbjct: 424 FGNIQQQETQVLYDLQNLAVSFVPTQC 435

BLAST of Spg002265 vs. ExPASy Swiss-Prot
Match: Q7XV21 (Aspartyl protease 37 OS=Oryza sativa subsp. japonica OX=39947 GN=AP37 PE=3 SV=2)

HSP 1 Score: 263.8 bits (673), Expect = 3.5e-69
Identity = 163/457 (35.67%), Postives = 239/457 (52.30%), Query Frame = 0

Query: 37  ALQQPESPSDGFRVRLNHVD----HVKNLTRFERLQRGVMRGRNRLQRLN-AMVLAANAA 96
           AL    +P   FR+ L  VD       NLT  E L+R + R R RL  +  A   AA+A 
Sbjct: 14  ALPASCAPPRSFRLELASVDASAADAANLTEHELLRRAIQRSRYRLAGIGMARGEAASAR 73

Query: 97  VGDQVQAPVVAGNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCKPCQQCFDQSTPIFDP 156
                + P++   GE+L+KL IG+PP  F+A +DT SDLIWTQC+PC  C+ Q  P+F+P
Sbjct: 74  KAVVAETPIMPAGGEYLVKLGIGTPPYKFTAAIDTASDLIWTQCQPCTGCYHQVDPMFNP 133

Query: 157 EESSSFSKLSCSSELCGALPTSTC---DGDSCEYFYTYGDFSSTHGVLAAETFTFGDSSD 216
             SS+++ L CSS+ C  L    C   D +SC+Y YTY   ++T G LA +    G+   
Sbjct: 134 RVSSTYAALPCSSDTCDELDVHRCGHDDDESCQYTYTYSGNATTEGTLAVDKLVIGED-- 193

Query: 217 DQVSISGVGFGCGDDNEGDG-FSQGAGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPS 276
              +  GV FGC   + G     Q +G+VGLGRGPLSLVSQL  ++FAYCL       P 
Sbjct: 194 ---AFRGVAFGCSTSSTGGAPPPQASGVVGLGRGPLSLVSQLSVRRFAYCLPPPASRIPG 253

Query: 277 SLLLGSLANVKPKTSQEEMKTTPLIRNPSQPSFYYLSLQGISVGGTQLSMPKAT------ 336
            L+LG+ A+     +       P+ R+P  PS+YYL+L G+ +G   +S+P  T      
Sbjct: 254 KLVLGADADAARNATNR--IAVPMRRDPRYPSYYYLNLDGLLIGDRAMSLPPTTTTTATA 313

Query: 337 -----------------FELRDDGSGGLIIDSGTTITYIEKSAFNLLKKEFIAQMKLPVH 396
                              + D    G+IID  +TIT++E S ++ L  +   +++LP  
Sbjct: 314 TATAPAPAPTPSPNATAVAVGDANRYGMIIDIASTITFLEASLYDELVNDLEVEIRLPRG 373

Query: 397 DSGTGGLDLCFNLP--AEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIG-- 456
              + GLDLCF LP      +V VP +   F G  L L        D  +G++CL +G  
Sbjct: 374 TGSSLGLDLCFILPDGVAFDRVYVPAVALAFDGRWLRLDKARLFAEDRESGMMCLMVGRA 433

Query: 457 SSSGMSIFGNLQQQNFMVVHDLQEETISFLPTQCDSI 458
            +  +SI GN QQQN  V+++L+   ++F+ + C ++
Sbjct: 434 EAGSVSILGNFQQQNMQVLYNLRRGRVTFVQSPCGAL 463

BLAST of Spg002265 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 1.7e-68
Identity = 144/368 (39.13%), Postives = 211/368 (57.34%), Query Frame = 0

Query: 94  DQVQAPVVA----GNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCKPCQQCFDQSTPIF 153
           + +  PVV+    G+GE+  ++ +G+P +    ++DTGSD+ W QC+PC  C+ QS P+F
Sbjct: 145 EDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVF 204

Query: 154 DPEESSSFSKLSCSSELCGALPTSTCDGDSCEYFYTYGDFSSTHGVLAAETFTFGDSSDD 213
           +P  SS++  L+CS+  C  L TS C  + C Y  +YGD S T G LA +T TFG+S   
Sbjct: 205 NPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSG-- 264

Query: 214 QVSISGVGFGCGDDNEGDGFSQGAGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSL 273
              I+ V  GCG DNEG  F+  AGL+GLG G LS+ +Q+K   F+YCL   D  K SSL
Sbjct: 265 --KINNVALGCGHDNEG-LFTGAAGLLGLGGGVLSITNQMKATSFSYCLVDRDSGKSSSL 324

Query: 274 LLGSLANVKPKTSQEEMKTTPLIRNPSQPSFYYLSLQGISVGGTQLSMPKATFELRDDGS 333
              S+             T PL+RN    +FYY+ L G SVGG ++ +P A F++   GS
Sbjct: 325 DFNSVQLGGGDA------TAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGS 384

Query: 334 GGLIIDSGTTITYIEKSAFNLLKKEFI-AQMKLPVHDSGTGGLDLCFNLPAEATQVEVPK 393
           GG+I+D GT +T ++  A+N L+  F+   + L    S     D C++  + +T V+VP 
Sbjct: 385 GGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLST-VKVPT 444

Query: 394 LTFHFKGA-DLELPGENYMIGDSRAGLICLAIG-SSSGMSIFGNLQQQNFMVVHDLQEET 453
           + FHF G   L+LP +NY+I    +G  C A   +SS +SI GN+QQQ   + +DL +  
Sbjct: 445 VAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNV 500

Query: 454 ISFLPTQC 455
           I     +C
Sbjct: 505 IGLSGNKC 500

BLAST of Spg002265 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 4.7e-66
Identity = 161/430 (37.44%), Postives = 233/430 (54.19%), Query Frame = 0

Query: 45  SDGFRVRLNHVDHV-KNLTRFE----RLQRGVMRGRNRLQRLNAMVLAAN-------AAV 104
           S    + L+H+D +  N T  E    RLQR   R ++ +  L A +   N          
Sbjct: 69  SSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKS-IATLAAQIPGRNVTHAPRPGGF 128

Query: 105 GDQVQAPVVAGNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCKPCQQCFDQSTPIFDPE 164
              V + +  G+GE+  +L +G+P R    ++DTGSD++W QC PC++C+ QS PIFDP 
Sbjct: 129 SSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPR 188

Query: 165 ESSSFSKLSCSSELCGALPTSTCD--GDSCEYFYTYGDFSSTHGVLAAETFTFGDSSDDQ 224
           +S +++ + CSS  C  L ++ C+    +C Y  +YGD S T G  + ET TF  +    
Sbjct: 189 KSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRN---- 248

Query: 225 VSISGVGFGCGDDNEGDGFSQGAGLVGLGRGPLSLVSQLK---EQKFAYCLT-AIDDSKP 284
             + GV  GCG DNEG  F   AGL+GLG+G LS   Q      QKF+YCL      SKP
Sbjct: 249 -RVKGVALGCGHDNEG-LFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKP 308

Query: 285 SSLLLGSLANVKPKTSQEEMKTTPLIRNPSQPSFYYLSLQGISVGGTQL-SMPKATFELR 344
           SS++ G+ A           + TPL+ NP   +FYY+ L GISVGGT++  +  + F+L 
Sbjct: 309 SSVVFGNAA------VSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLD 368

Query: 345 DDGSGGLIIDSGTTITYIEKSAFNLLKKEFIAQMKLPVHDSGTGGLDLCFNLPAEATQVE 404
             G+GG+IIDSGT++T + + A+  ++  F    K           D CF+L +   +V+
Sbjct: 369 QIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDL-SNMNEVK 428

Query: 405 VPKLTFHFKGADLELPGENYMIGDSRAGLICLAI-GSSSGMSIFGNLQQQNFMVVHDLQE 455
           VP +  HF+GAD+ LP  NY+I     G  C A  G+  G+SI GN+QQQ F VV+DL  
Sbjct: 429 VPTVVLHFRGADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLAS 484

BLAST of Spg002265 vs. ExPASy TrEMBL
Match: A0A6J1EPU6 (aspartic proteinase nepenthesin-1 OS=Cucurbita moschata OX=3662 GN=LOC111435534 PE=3 SV=1)

HSP 1 Score: 770.8 bits (1989), Expect = 3.2e-219
Identity = 387/451 (85.81%), Postives = 410/451 (90.91%), Query Frame = 0

Query: 7   SFRYPIAMVVLVTTLFTGTSSALSSTRSRRALQQPESPSDGFRVRLNHVDHVKNLTRFER 66
           S RY I  VVL  T+    +SA SS+ SRRAL QP+ PSDGFRV LNHVDHVKNLTRFER
Sbjct: 4   SLRYLIISVVLSITMLFIHTSASSSSLSRRALWQPKLPSDGFRVSLNHVDHVKNLTRFER 63

Query: 67  LQRGVMRGRNRLQRLNAMVLAANAAVGDQVQAPVVAGNGEFLMKLAIGSPPRSFSAIMDT 126
           LQRGV RG+ RL RLNAMVLAAN  VG +VQAPVVAGNGEFLMKLAIGSPPRSFSAIMDT
Sbjct: 64  LQRGVARGKTRLHRLNAMVLAANVGVGGRVQAPVVAGNGEFLMKLAIGSPPRSFSAIMDT 123

Query: 127 GSDLIWTQCKPCQQCFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTCDGDSCEYFYTY 186
           GSDLIWTQCKPCQQCFDQ+TPIFDP+ESSSFSK+SCSSELC ALPTSTC  D CEYFYTY
Sbjct: 124 GSDLIWTQCKPCQQCFDQATPIFDPKESSSFSKISCSSELCDALPTSTCSSDECEYFYTY 183

Query: 187 GDFSSTHGVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGLVGLGRGPLSLV 246
           GD+SSTHGVLAAETFTFGDSS DQVSI G+GFGCGDDNEGDGFSQG GLVGLGRGPLSLV
Sbjct: 184 GDYSSTHGVLAAETFTFGDSSQDQVSIPGLGFGCGDDNEGDGFSQGEGLVGLGRGPLSLV 243

Query: 247 SQLKEQKFAYCLTAIDDSKPSSLLLGSLANVKPKTSQEEMKTTPLIRNPSQPSFYYLSLQ 306
           SQLKEQKF+YCLTAIDD+KPSSLL+GSLANVKPK S+ E+KTTPLIRNPSQPSFYYLSLQ
Sbjct: 244 SQLKEQKFSYCLTAIDDTKPSSLLMGSLANVKPKASEGEIKTTPLIRNPSQPSFYYLSLQ 303

Query: 307 GISVGGTQLSMPKATFELRDDGSGGLIIDSGTTITYIEKSAFNLLKKEFIAQMKLPVHDS 366
           GISVGGTQL +PKATFEL DDGSGG+IIDSGTTITYIEK+AF LLKKEF++QMKLPV DS
Sbjct: 304 GISVGGTQLPIPKATFELHDDGSGGVIIDSGTTITYIEKNAFTLLKKEFVSQMKLPVDDS 363

Query: 367 GTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSSGMS 426
           GT GLDLCFNLP E TQVEVPKLTFHFKGADLELPGENYMIGDS+A LICLAIGSSSGMS
Sbjct: 364 GTSGLDLCFNLPPETTQVEVPKLTFHFKGADLELPGENYMIGDSKAELICLAIGSSSGMS 423

Query: 427 IFGNLQQQNFMVVHDLQEETISFLPTQCDSI 458
           IFGNLQQQN MVVHDLQEET+SFLPTQC  I
Sbjct: 424 IFGNLQQQNIMVVHDLQEETVSFLPTQCSEI 454

BLAST of Spg002265 vs. ExPASy TrEMBL
Match: A0A6J1DKZ2 (aspartic proteinase nepenthesin-1 OS=Momordica charantia OX=3673 GN=LOC111022058 PE=3 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 7.2e-219
Identity = 390/460 (84.78%), Postives = 419/460 (91.09%), Query Frame = 0

Query: 1   MTNSLRSFRYPIAMVVLVTTLFTGTSSALSSTRSRRALQQPESPSDGFRVRLNHVD-HVK 60
           M +SL S RYPI +V ++ TLF    +  SST SRRALQQ +  ++GFR+RL+HVD HVK
Sbjct: 1   MLDSLCSVRYPIVLVAVLATLFFIDLTVSSSTLSRRALQQQKLLNNGFRMRLHHVDHHVK 60

Query: 61  NLTRFERLQRGVMRGRNRLQRLNAMVLAA--NAAVGDQVQAPVVAGNGEFLMKLAIGSPP 120
           NLTRFERLQRG  RGRNRLQRLNAMVLAA   AAVGDQVQAPVVAGNGEFLMKLAIGSPP
Sbjct: 61  NLTRFERLQRGAARGRNRLQRLNAMVLAAAGGAAVGDQVQAPVVAGNGEFLMKLAIGSPP 120

Query: 121 RSFSAIMDTGSDLIWTQCKPCQQCFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTCDG 180
           +SFSAIMDTGSDLIWTQCKPCQQCFDQSTPIFDP+ESSSFSKLSCSSELCGALPTS C  
Sbjct: 121 KSFSAIMDTGSDLIWTQCKPCQQCFDQSTPIFDPKESSSFSKLSCSSELCGALPTSACSN 180

Query: 181 DSCEYFYTYGDFSSTHGVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGLVG 240
           D CEY YTYGD+SSTHG+L AETFTFGD  +DQVSIS +GFGCGDDNEGDGFSQGAGLVG
Sbjct: 181 DGCEYLYTYGDYSSTHGLLGAETFTFGDPGEDQVSISEIGFGCGDDNEGDGFSQGAGLVG 240

Query: 241 LGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANVKPKTSQEEMKTTPLIRNPSQ 300
           LGRGPLSLVSQLKEQKFAYCLT IDDSKPSSLL+GSLANVKPK SQ+E+KTTPLIRNPSQ
Sbjct: 241 LGRGPLSLVSQLKEQKFAYCLTPIDDSKPSSLLMGSLANVKPKKSQDEIKTTPLIRNPSQ 300

Query: 301 PSFYYLSLQGISVGGTQLSMPKATFELRDDGSGGLIIDSGTTITYIEKSAFNLLKKEFIA 360
           PSFYYLSL+GISVGG+ L++P+ TFELRDDGSGG+IIDSGTTITYI+KSAF LLKKEFIA
Sbjct: 301 PSFYYLSLEGISVGGSHLAIPQPTFELRDDGSGGVIIDSGTTITYIDKSAFTLLKKEFIA 360

Query: 361 QMKLPVHDSGTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICL 420
           QMKLPV DSGTGGLDLCF LP+EATQVEVPKLTFHFK ADLELPGENYMIGDS AGL+CL
Sbjct: 361 QMKLPVDDSGTGGLDLCFKLPSEATQVEVPKLTFHFKDADLELPGENYMIGDSAAGLLCL 420

Query: 421 AIGSSSGMSIFGNLQQQNFMVVHDLQEETISFLPTQCDSI 458
           AIGSSSGMSIFGNLQQQNFMVVHDLQEETISF+PTQCD I
Sbjct: 421 AIGSSSGMSIFGNLQQQNFMVVHDLQEETISFVPTQCDGI 460

BLAST of Spg002265 vs. ExPASy TrEMBL
Match: A0A0A0KYT9 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G554680 PE=3 SV=1)

HSP 1 Score: 763.5 bits (1970), Expect = 5.2e-217
Identity = 387/458 (84.50%), Postives = 416/458 (90.83%), Query Frame = 0

Query: 4   SLRSFRY--PIAMVVLVTTLFTGTSSALSSTRSRRALQQPES-PSDGFRVRLNHVDHVKN 63
           SLRSF Y   + +++L+TTLF  T  A SS+ SRRALQ+P   PS GFRVRL HVDHVKN
Sbjct: 5   SLRSFGYLHRLLLIILITTLFINT-LAFSSSLSRRALQKPNKLPSHGFRVRLKHVDHVKN 64

Query: 64  LTRFERLQRGVMRGRNRLQRLNAMVL-AANAAVGDQVQAPVVAGNGEFLMKLAIGSPPRS 123
           LTRFERL+RGV RG+NRL RLNAMVL AANA VGDQV+APVVAGNGEFLMKLAIGSPPRS
Sbjct: 65  LTRFERLRRGVARGKNRLHRLNAMVLAAANATVGDQVKAPVVAGNGEFLMKLAIGSPPRS 124

Query: 124 FSAIMDTGSDLIWTQCKPCQQCFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTCDGDS 183
           FSAIMDTGSDLIWTQCKPCQQCFDQSTPIFDP++SSSF K+SCSSELCGALPTSTC  D 
Sbjct: 125 FSAIMDTGSDLIWTQCKPCQQCFDQSTPIFDPKQSSSFYKISCSSELCGALPTSTCSSDG 184

Query: 184 CEYFYTYGDFSSTHGVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGLVGLG 243
           CEY YTYGD SST GVLA ETFTFGDS++DQ+SI G+GFGCG+DN GDGFSQGAGLVGLG
Sbjct: 185 CEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLVGLG 244

Query: 244 RGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANVKPKTSQEEMKTTPLIRNPSQPS 303
           RGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLAN+ PKTS++EMKTTPLI+NPSQPS
Sbjct: 245 RGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKTTPLIKNPSQPS 304

Query: 304 FYYLSLQGISVGGTQLSMPKATFELRDDGSGGLIIDSGTTITYIEKSAFNLLKKEFIAQM 363
           FYYLSLQGISVGGTQLS+PK+TFEL DDGSGG+IIDSGTTITY+E SAF  LK EFIAQM
Sbjct: 305 FYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYVENSAFTSLKNEFIAQM 364

Query: 364 KLPVHDSGTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAI 423
            LPV DSGTGGLDLCFNLPA   QVEVPKLTFHFKGADLELPGENYMIGDS+AGL+CLAI
Sbjct: 365 NLPVDDSGTGGLDLCFNLPAGTNQVEVPKLTFHFKGADLELPGENYMIGDSKAGLLCLAI 424

Query: 424 GSSSGMSIFGNLQQQNFMVVHDLQEETISFLPTQCDSI 458
           GSS GMSIFGNLQQQNFMVVHDLQEET+SFLPTQCDSI
Sbjct: 425 GSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

BLAST of Spg002265 vs. ExPASy TrEMBL
Match: A0A6J1HXS0 (aspartic proteinase nepenthesin-1 OS=Cucurbita maxima OX=3661 GN=LOC111467205 PE=3 SV=1)

HSP 1 Score: 762.3 bits (1967), Expect = 1.2e-216
Identity = 382/451 (84.70%), Postives = 407/451 (90.24%), Query Frame = 0

Query: 7   SFRYPIAMVVLVTTLFTGTSSALSSTRSRRALQQPESPSDGFRVRLNHVDHVKNLTRFER 66
           S RY I  VVL  T+    +SA SS+ SRRAL+QP+ PSDGFRV LNHVDHVKNLTRFER
Sbjct: 4   SLRYLIVSVVLSITMLFIHTSASSSSLSRRALRQPKLPSDGFRVSLNHVDHVKNLTRFER 63

Query: 67  LQRGVMRGRNRLQRLNAMVLAANAAVGDQVQAPVVAGNGEFLMKLAIGSPPRSFSAIMDT 126
           LQRGV RG+ RL RLNAM+LAAN  VG +VQAPVVAGNGEFLMKLAIGSPPRSFSAIMDT
Sbjct: 64  LQRGVARGKTRLHRLNAMMLAANIGVGGRVQAPVVAGNGEFLMKLAIGSPPRSFSAIMDT 123

Query: 127 GSDLIWTQCKPCQQCFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTCDGDSCEYFYTY 186
           GSDLIWTQCKPCQQCFDQ+TPIFDP+ESSSFSK+SCSSELC ALPTSTC  D CEYFYTY
Sbjct: 124 GSDLIWTQCKPCQQCFDQATPIFDPKESSSFSKISCSSELCDALPTSTCSSDECEYFYTY 183

Query: 187 GDFSSTHGVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGLVGLGRGPLSLV 246
           GD+SSTHGVLAAETFTFGDSS DQVSI G+GFGCGDDNEGDGFSQG GLVGLGRGPLSLV
Sbjct: 184 GDYSSTHGVLAAETFTFGDSSQDQVSIPGLGFGCGDDNEGDGFSQGEGLVGLGRGPLSLV 243

Query: 247 SQLKEQKFAYCLTAIDDSKPSSLLLGSLANVKPKTSQEEMKTTPLIRNPSQPSFYYLSLQ 306
           SQLKEQKF+YCLTAIDD+KPSSLLLGSLANVKPK S  E+KTTPLIRNPSQPSFYYLSLQ
Sbjct: 244 SQLKEQKFSYCLTAIDDTKPSSLLLGSLANVKPKASDGEIKTTPLIRNPSQPSFYYLSLQ 303

Query: 307 GISVGGTQLSMPKATFELRDDGSGGLIIDSGTTITYIEKSAFNLLKKEFIAQMKLPVHDS 366
           GISVGGTQL +PK TFEL DDGSGG+IIDSGTTITYIEK+AF LLKKEF++QMKLPV DS
Sbjct: 304 GISVGGTQLPIPKNTFELHDDGSGGVIIDSGTTITYIEKNAFTLLKKEFVSQMKLPVDDS 363

Query: 367 GTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSSGMS 426
           GT GLDLCFNLP +  QVEVPKLTFHFKGADLELPGENYMIGDS+A LICL IGSS+GMS
Sbjct: 364 GTSGLDLCFNLPPKTNQVEVPKLTFHFKGADLELPGENYMIGDSKAELICLTIGSSNGMS 423

Query: 427 IFGNLQQQNFMVVHDLQEETISFLPTQCDSI 458
           IFGNLQQQN MVVHDLQEET+SFLPTQC  I
Sbjct: 424 IFGNLQQQNIMVVHDLQEETVSFLPTQCSDI 454

BLAST of Spg002265 vs. ExPASy TrEMBL
Match: A0A5A7TD10 (Aspartic proteinase nepenthesin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold128G001020 PE=3 SV=1)

HSP 1 Score: 757.3 bits (1954), Expect = 3.7e-215
Identity = 384/458 (83.84%), Postives = 415/458 (90.61%), Query Frame = 0

Query: 4   SLRSFRY--PIAMVVLVTTLFTGTSSALSSTRSRRALQQPES-PSDGFRVRLNHVDHVKN 63
           SLRSF Y   + ++V +TTLF  T  A SS+ S RALQ+P   PS GFRVRL HVDHVKN
Sbjct: 5   SLRSFGYLQLLLLIVFITTLFINT-LAFSSSLSTRALQKPNKLPSHGFRVRLKHVDHVKN 64

Query: 64  LTRFERLQRGVMRGRNRLQRLNAMVL-AANAAVGDQVQAPVVAGNGEFLMKLAIGSPPRS 123
           LTRFERL+RGV RG+NRL RLNAMVL AANA+VGDQV+APVVAGNGEFLMKLAIGSPPRS
Sbjct: 65  LTRFERLRRGVARGKNRLHRLNAMVLAAANASVGDQVKAPVVAGNGEFLMKLAIGSPPRS 124

Query: 124 FSAIMDTGSDLIWTQCKPCQQCFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTCDGDS 183
           FSAIMDTGSDLIWTQCKPCQQCFDQ+TPIFDP++SSSFSK+SC SELCGALPTSTC  D 
Sbjct: 125 FSAIMDTGSDLIWTQCKPCQQCFDQATPIFDPKQSSSFSKISCRSELCGALPTSTCSSDG 184

Query: 184 CEYFYTYGDFSSTHGVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGLVGLG 243
           CEY YTYGD SST GVLA ETFTFGDS++DQ+SI G+GFGCG+DN GDGFSQGAGLVGLG
Sbjct: 185 CEYLYTYGDSSSTQGVLAFETFTFGDSTEDQISIPGLGFGCGNDNNGDGFSQGAGLVGLG 244

Query: 244 RGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANVKPKTSQEEMKTTPLIRNPSQPS 303
           RGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLAN+ PKTS++EMK TPLI+NPSQPS
Sbjct: 245 RGPLSLVSQLKEQKFAYCLTAIDDSKPSSLLLGSLANITPKTSKDEMKATPLIKNPSQPS 304

Query: 304 FYYLSLQGISVGGTQLSMPKATFELRDDGSGGLIIDSGTTITYIEKSAFNLLKKEFIAQM 363
           FYYLSLQGISVGGTQLS+PK+TFEL DDGSGG+IIDSGTTITYIE +AF+ LK EFIAQM
Sbjct: 305 FYYLSLQGISVGGTQLSIPKSTFELHDDGSGGVIIDSGTTITYIESTAFSSLKNEFIAQM 364

Query: 364 KLPVHDSGTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAI 423
            LPV DSGTGGLDLCFNLPA  TQVEVPKLTFHFKGADLELPGENYMIGDS+ GL+CLAI
Sbjct: 365 NLPVDDSGTGGLDLCFNLPAGTTQVEVPKLTFHFKGADLELPGENYMIGDSKTGLLCLAI 424

Query: 424 GSSSGMSIFGNLQQQNFMVVHDLQEETISFLPTQCDSI 458
           GSS GMSIFGNLQQQNFMVVHDLQEET+SFLPTQCDSI
Sbjct: 425 GSSRGMSIFGNLQQQNFMVVHDLQEETLSFLPTQCDSI 461

BLAST of Spg002265 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 544.3 bits (1401), Expect = 9.5e-155
Identity = 281/468 (60.04%), Postives = 358/468 (76.50%), Query Frame = 0

Query: 2   TNSLRSFRYPIAMVVLVTTLFTGTSSALSSTRSRRALQQPES-PSDGFRVRLNHVDHVKN 61
           ++S  S  +P  ++     LF+   S  SS RS      P++ P  GFR+ L HVD  KN
Sbjct: 3   SSSSSSLLFPFFLI-----LFSCLISVSSSRRSLIDRTLPKNLPRSGFRLSLRHVDSGKN 62

Query: 62  LTRFERLQRGVMRGRNRLQRLNAMVLAANAAVGD---QVQAPVVAGNGEFLMKLAIGSPP 121
           LT+ +++QRG+ RG +RL RL A+ + A A+  D    ++AP   G+GEFLM+L+IG+P 
Sbjct: 63  LTKIQKIQRGINRGFHRLNRLGAVAVLAVASKPDDTNNIKAPTHGGSGEFLMELSIGNPA 122

Query: 122 RSFSAIMDTGSDLIWTQCKPCQQCFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTC-- 181
             +SAI+DTGSDLIWTQCKPC +CFDQ TPIFDPE+SSS+SK+ CSS LC ALP S C  
Sbjct: 123 VKYSAIVDTGSDLIWTQCKPCTECFDQPTPIFDPEKSSSYSKVGCSSGLCNALPRSNCNE 182

Query: 182 DGDSCEYFYTYGDFSSTHGVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGL 241
           D D+CEY YTYGD+SST G+LA ETFTF    +D+ SISG+GFGCG +NEGDGFSQG+GL
Sbjct: 183 DKDACEYLYTYGDYSSTRGLLATETFTF----EDENSISGIGFGCGVENEGDGFSQGSGL 242

Query: 242 VGLGRGPLSLVSQLKEQKFAYCLTAIDDSK-PSSLLLGSLAN-VKPKTSQ----EEMKTT 301
           VGLGRGPLSL+SQLKE KF+YCLT+I+DS+  SSL +GSLA+ +  KT      E  KT 
Sbjct: 243 VGLGRGPLSLISQLKETKFSYCLTSIEDSEASSSLFIGSLASGIVNKTGASLDGEVTKTM 302

Query: 302 PLIRNPSQPSFYYLSLQGISVGGTQLSMPKATFELRDDGSGGLIIDSGTTITYIEKSAFN 361
            L+RNP QPSFYYL LQGI+VG  +LS+ K+TFEL +DG+GG+IIDSGTTITY+E++AF 
Sbjct: 303 SLLRNPDQPSFYYLELQGITVGAKRLSVEKSTFELAEDGTGGMIIDSGTTITYLEETAFK 362

Query: 362 LLKKEFIAQMKLPVHDSGTGGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGD 421
           +LK+EF ++M LPV DSG+ GLDLCF LP  A  + VPK+ FHFKGADLELPGENYM+ D
Sbjct: 363 VLKEEFTSRMSLPVDDSGSTGLDLCFKLPDAAKNIAVPKMIFHFKGADLELPGENYMVAD 422

Query: 422 SRAGLICLAIGSSSGMSIFGNLQQQNFMVVHDLQEETISFLPTQCDSI 458
           S  G++CLA+GSS+GMSIFGN+QQQNF V+HDL++ET+SF+PT+C  +
Sbjct: 423 SSTGVLCLAMGSSNGMSIFGNVQQQNFNVLHDLEKETVSFVPTECGKL 461

BLAST of Spg002265 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 265.0 bits (676), Expect = 1.1e-70
Identity = 168/451 (37.25%), Postives = 236/451 (52.33%), Query Frame = 0

Query: 30  SSTRSRRALQQPESPSDGFRVRLN---------HVDHVKNLTRFERLQRGVMRGRNRLQR 89
           SS R  +  +Q  S S  F ++L+         H D+ K+LT   RL R   R ++ + R
Sbjct: 49  SSFRLNQQEEQTHSASSSFSLQLHSRVSVRGTEHSDY-KSLT-LARLNRDTARVKSLITR 108

Query: 90  LNAMVLAANAA-----------VGDQVQAPVVA----GNGEFLMKLAIGSPPRSFSAIMD 149
           L+  +   + A               ++AP+++    G+GE+  ++ IG P R    ++D
Sbjct: 109 LDLAINNISKADLKPISTMYTTEEQDIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLD 168

Query: 150 TGSDLIWTQCKPCQQCFDQSTPIFDPEESSSFSKLSCSSELCGALPTSTCDGDSCEYFYT 209
           TGSD+ W QC PC  C+ Q+ PIF+P  SSS+  LSC +  C AL  S C   +C Y  +
Sbjct: 169 TGSDVNWLQCTPCADCYHQTEPIFEPSSSSSYEPLSCDTPQCNALEVSECRNATCLYEVS 228

Query: 210 YGDFSSTHGVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGLVGLGRGPLSL 269
           YGD S T G  A ET T G +      +  V  GCG  NEG  F   AGL+GLG G L+L
Sbjct: 229 YGDGSYTVGDFATETLTIGST-----LVQNVAVGCGHSNEG-LFVGAAGLLGLGGGLLAL 288

Query: 270 VSQLKEQKFAYCLTAIDDSKPSSLLLGSLANVKPKTSQEEMKTTPLIRNPSQPSFYYLSL 329
            SQL    F+YCL   D    S++  G+       +   +    PL+RN    +FYYL L
Sbjct: 289 PSQLNTTSFSYCLVDRDSDSASTVDFGT-------SLSPDAVVAPLLRNHQLDTFYYLGL 348

Query: 330 QGISVGGTQLSMPKATFELRDDGSGGLIIDSGTTITYIEKSAFNLLKKEFIAQMKLPVHD 389
            GISVGG  L +P+++FE+ + GSGG+IIDSGT +T ++   +N L+  F+         
Sbjct: 349 TGISVGGELLQIPQSSFEMDESGSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKA 408

Query: 390 SGTGGLDLCFNLPAEATQVEVPKLTFHFKGAD-LELPGENYMIGDSRAGLICLAIG-SSS 449
           +G    D C+NL A+ T VEVP + FHF G   L LP +NYMI     G  CLA   ++S
Sbjct: 409 AGVAMFDTCYNLSAKTT-VEVPTVAFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTAS 468

Query: 450 GMSIFGNLQQQNFMVVHDLQEETISFLPTQC 455
            ++I GN+QQQ   V  DL    I F   +C
Sbjct: 469 SLAIIGNVQQQGTRVTFDLANSLIGFSSNKC 483

BLAST of Spg002265 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 261.5 bits (667), Expect = 1.2e-69
Identity = 144/368 (39.13%), Postives = 211/368 (57.34%), Query Frame = 0

Query: 94  DQVQAPVVA----GNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCKPCQQCFDQSTPIF 153
           + +  PVV+    G+GE+  ++ +G+P +    ++DTGSD+ W QC+PC  C+ QS P+F
Sbjct: 145 EDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVF 204

Query: 154 DPEESSSFSKLSCSSELCGALPTSTCDGDSCEYFYTYGDFSSTHGVLAAETFTFGDSSDD 213
           +P  SS++  L+CS+  C  L TS C  + C Y  +YGD S T G LA +T TFG+S   
Sbjct: 205 NPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSFTVGELATDTVTFGNSG-- 264

Query: 214 QVSISGVGFGCGDDNEGDGFSQGAGLVGLGRGPLSLVSQLKEQKFAYCLTAIDDSKPSSL 273
              I+ V  GCG DNEG  F+  AGL+GLG G LS+ +Q+K   F+YCL   D  K SSL
Sbjct: 265 --KINNVALGCGHDNEG-LFTGAAGLLGLGGGVLSITNQMKATSFSYCLVDRDSGKSSSL 324

Query: 274 LLGSLANVKPKTSQEEMKTTPLIRNPSQPSFYYLSLQGISVGGTQLSMPKATFELRDDGS 333
              S+             T PL+RN    +FYY+ L G SVGG ++ +P A F++   GS
Sbjct: 325 DFNSVQLGGGDA------TAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGS 384

Query: 334 GGLIIDSGTTITYIEKSAFNLLKKEFI-AQMKLPVHDSGTGGLDLCFNLPAEATQVEVPK 393
           GG+I+D GT +T ++  A+N L+  F+   + L    S     D C++  + +T V+VP 
Sbjct: 385 GGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLST-VKVPT 444

Query: 394 LTFHFKGA-DLELPGENYMIGDSRAGLICLAIG-SSSGMSIFGNLQQQNFMVVHDLQEET 453
           + FHF G   L+LP +NY+I    +G  C A   +SS +SI GN+QQQ   + +DL +  
Sbjct: 445 VAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNV 500

Query: 454 ISFLPTQC 455
           I     +C
Sbjct: 505 IGLSGNKC 500

BLAST of Spg002265 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 253.4 bits (646), Expect = 3.4e-67
Identity = 161/430 (37.44%), Postives = 233/430 (54.19%), Query Frame = 0

Query: 45  SDGFRVRLNHVDHV-KNLTRFE----RLQRGVMRGRNRLQRLNAMVLAAN-------AAV 104
           S    + L+H+D +  N T  E    RLQR   R ++ +  L A +   N          
Sbjct: 69  SSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKS-IATLAAQIPGRNVTHAPRPGGF 128

Query: 105 GDQVQAPVVAGNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCKPCQQCFDQSTPIFDPE 164
              V + +  G+GE+  +L +G+P R    ++DTGSD++W QC PC++C+ QS PIFDP 
Sbjct: 129 SSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPR 188

Query: 165 ESSSFSKLSCSSELCGALPTSTCD--GDSCEYFYTYGDFSSTHGVLAAETFTFGDSSDDQ 224
           +S +++ + CSS  C  L ++ C+    +C Y  +YGD S T G  + ET TF  +    
Sbjct: 189 KSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRN---- 248

Query: 225 VSISGVGFGCGDDNEGDGFSQGAGLVGLGRGPLSLVSQLK---EQKFAYCLT-AIDDSKP 284
             + GV  GCG DNEG  F   AGL+GLG+G LS   Q      QKF+YCL      SKP
Sbjct: 249 -RVKGVALGCGHDNEG-LFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKP 308

Query: 285 SSLLLGSLANVKPKTSQEEMKTTPLIRNPSQPSFYYLSLQGISVGGTQL-SMPKATFELR 344
           SS++ G+ A           + TPL+ NP   +FYY+ L GISVGGT++  +  + F+L 
Sbjct: 309 SSVVFGNAA------VSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLD 368

Query: 345 DDGSGGLIIDSGTTITYIEKSAFNLLKKEFIAQMKLPVHDSGTGGLDLCFNLPAEATQVE 404
             G+GG+IIDSGT++T + + A+  ++  F    K           D CF+L +   +V+
Sbjct: 369 QIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDL-SNMNEVK 428

Query: 405 VPKLTFHFKGADLELPGENYMIGDSRAGLICLAI-GSSSGMSIFGNLQQQNFMVVHDLQE 455
           VP +  HF+GAD+ LP  NY+I     G  C A  G+  G+SI GN+QQQ F VV+DL  
Sbjct: 429 VPTVVLHFRGADVSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLAS 484

BLAST of Spg002265 vs. TAIR 10
Match: AT5G33340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 250.4 bits (638), Expect = 2.8e-66
Identity = 157/446 (35.20%), Postives = 235/446 (52.69%), Query Frame = 0

Query: 18  VTTLFTGTSSALSSTRSRRALQQPESPSDGFRVRLNHVDHVKNLTRFERLQRGVMRGRNR 77
           + +LF+    +L    S         P  GF   L H D  K+   +  ++    R RN 
Sbjct: 1   MASLFSSVLLSLCLLSSLFLSNANAKPKLGFTADLIHRDSPKS-PFYNPMETSSQRLRNA 60

Query: 78  LQR-LNAMVLAANAAVGDQVQAPVVAGNGEFLMKLAIGSPPRSFSAIMDTGSDLIWTQCK 137
           + R +N +          Q Q  + + +GE+LM ++IG+PP    AI DTGSDL+WTQC 
Sbjct: 61  IHRSVNRVFHFTEKDNTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCA 120

Query: 138 PCQQCFDQSTPIFDPEESSSFSKLSCSSELCGAL---PTSTCDGDSCEYFYTYGDFSSTH 197
           PC  C+ Q  P+FDP+ SS++  +SCSS  C AL    + + + ++C Y  +YGD S T 
Sbjct: 121 PCDDCYTQVDPLFDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTK 180

Query: 198 GVLAAETFTFGDSSDDQVSISGVGFGCGDDNEGDGFSQGAGLVGLGRGPLSLVSQLKEQ- 257
           G +A +T T G S    + +  +  GCG +N G    +G+G+VGLG GP+SL+ QL +  
Sbjct: 181 GNIAVDTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSI 240

Query: 258 --KFAYCLTAIDDSK--PSSLLLGSLANVKPKTSQEEMKTTPLIRNPSQPSFYYLSLQGI 317
             KF+YCL  +   K   S +  G+ A V    S   + +TPLI   SQ +FYYL+L+ I
Sbjct: 241 DGKFSYCLVPLTSKKDQTSKINFGTNAIV----SGSGVVSTPLIAKASQETFYYLTLKSI 300

Query: 318 SVGGTQLSMPKATFELRDDGSGGLIIDSGTTITYIEKSAFNLLKKEFIAQMKLPVHDSGT 377
           SVG  Q+    +  E      G +IIDSGTT+T +    ++ L+    + +         
Sbjct: 301 SVGSKQIQYSGSDSE---SSEGNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQ 360

Query: 378 GGLDLCFNLPAEATQVEVPKLTFHFKGADLELPGENYMIGDSRAGLICLAIGSSSGMSIF 437
            GL LC++   +   ++VP +T HF GAD++L   N  +  S   L+C A   S   SI+
Sbjct: 361 SGLSLCYSATGD---LKVPVITMHFDGADVKLDSSNAFVQVSE-DLVCFAFRGSPSFSIY 420

Query: 438 GNLQQQNFMVVHDLQEETISFLPTQC 455
           GN+ Q NF+V +D   +T+SF PT C
Sbjct: 421 GNVAQMNFLVGYDTVSKTVSFKPTDC 434