Spg002116 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg002116
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Asparagine--tRNA ligase
Location	scaffold2: 27387616 .. 27391116 (+)
RNA-Seq Expression	Spg002116
Synteny	Spg002116

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTGCAGTGGGCGAGAAGATACACCGGGAACGGACCAATGGCAGAGCATTCCGCGCCACCCGCCGACCAACTCGCCGCCGTCGCCTTGAACGACGATGCTTCCACTTACAAATCTCTCAAAGCTGAATTCTCCGATCGCGTCCCGATCCGTTCAATCGTCTGCCGGCCTGACGGCGGCGCTGGACTCGCCGGCCAGAAGGTTCGCGTCGGCGGCTGGGTCAAAACTGGAAGGAAGGCCGACAAGGACGCTTTTGCCTTTCTCGAGTTGAACGATGGCTCTTGCCCTCAGAATCTCCAAGTGATCATCGAAGCGGCTGTGGCGGATCTTGGCCAGATTGTTCCGACTGGAACCTGCGTTGTTGTTGACGGCGTTTTCAAACTTCCTCCGCCGGATAAGCACCAGAAAGTCGAGCTTCGGGCTGAGGCTGTCGTCCATGTCGGTCCCGTTGATCCTGCGCGGTATCCTTTGCCGAAGACGAAACTCACTCTCGAGTTTCTCAGAGATTTCGTTCATCTCCGCCCTAGAACCAACACGGTATAAGAACGAATCCGTCTCTCGAATTTGAATTCATTAATTGGATCAAATCTGTTTATTTCCGCATTTCTGCATTTCATTTCTTATATTGTTCTGTAACTTAGCATTTAAATGGTGTTGTTCTTCTCTGATGCTGATAGAAAGGTCCAATTTATAGAACAAATACATTTCTATTGATGAGTTTGTGTTTTTTCTACGATTACAGATATCTGCGGTTGCTAGAATTCGAAATGCACTTGCATATGCCACTCATACATTTTTTAACAAACATGGATTTCTGTATATTCACACGCCAATTGTCACCACAAGCGATTGTGAGGGTGCGGGTGAAATGTTTCAAGTTACCACTCTATTGAGTGAGGCTGAAAGATTAGAGAAGGAGTTGATTAAAAACCCACCTCCATCAGAAGCAGAAGTAGATGAAGCAAGGAAAATTGTCAAGGAGAAAGGAGAAGCTGTTGCAAAGCTGAAGGCTTCTAAGGCGAGCAAGGAGGAAATTGGTGCCTCTGTTTTGGAACTGAAGAAAGCAAAGGAGAATGTTGCTCGGTTGGAAGAGAGAGCAAAGCTTAAACCAGGCATCCCAGAGAAAGATGGGAAAGTTGATTATGGACGGGATTTCTTTGCTCGGCAAGCGTTTTTGACTGTTTCTGGCCAGCTGCAAGTTGAAACTTATGCCTGTGCTCTTAGCAGTGTATACACATTTGGACCGACCTTCCGTGCCGAAAACTCTCACACTTCAAGACATCTTGCAGAGTTTTGGATGGTTGAGCCTGAAATTGCATTTGCAGATTTGCAGGTTTGTTACTATGCTGAAATTGTATTCTAAGTTGTATCTTTTTCTGCGTACTCAATTGAAATTGAATTCATGGCTCTGTCTCAGAAGAGTAGTCATGTTTAACCATCTCAAGACACAATACCTTTGACGCTTATTTTATAGTTGAATTTTTATGTTCTAGGATGAGCTTATCTTGCTTTAGTCTTGCAATTATCTTCTATAAGAAAATCACTCTGGGTTGGCCTAGTAGTTAATAAGGACCAATGTGAATAATAAAGTGCTTAGAGGGAATGAATTCAAGCTATGGTGGCTACTTACTTGGGATTTAATATCATCGAGTTTCCTTAGTGTTAGTCAAGTTGCACTCATGGATGTAAAAAGAAAAGGTCTTCTATAAGATATTAGGTATGGTCCTTGTAAGATATATTTTCCGCAAGAACTGTAACTTAAGTTGGCTGTATTCTCTATGCTACTAAGCAATATGGCAAACAACCATATTCAAAAGAGAGCTATGTTTGGAAATTCTTTTAGGAATAAGAAAAAACAATGGAACTCTGCAATATAGGGTTTAGTTCTCTTTTGTTGATCGGATGCTCTTCTTCTAGAGTTTTAATTGATTTAGCCATATGTAACATCTTTTTCTTTAATTGGTTGAAGGATGATATGAATTGTGCGGAAGCATATGTGAGATTTCTGTGTCAGTGGTTACTTGACAACTGCTTAGATGACATGAAATTTATGGCTGACAAGTACGACCGAAGTTGTATTGATCGTCTCAAAATGGTCTCTTCAACCCCTTTCGTAAGAACTTCCTATACGGAAGCTGTTGAAATACTTGAGGAAGCCGTTAAGAAGGGTAAGCAGTTTGAGAACAAAGTGGAATGGGGAATTGATTTGGCTTCTGAGCATGAAAGGTTCGTGAACTCCCATCCTTTTCATTTTCATTCCACTTGAACAATGTTTTTTAATCTCATTTTTTTTTCTGCTTTGCTTGCTCAGATACTTGACTGAGGTGGTATTTAAAAAGCCTGTCATTGTCTACAACTATCCAAAGGGAATCAAAGCATTTTATATGAGGCTCAATGATGACTCAAAGACTGTAGCTGCAATGGATGTTCTTGTACCTAAGGTAGTTTATTTTGTTCCAATGTTTCTTCAATAACTGTGATTCATTTCACCATTTTGACGCTTTTGTTTGTGAAAATCGAAATCTTTCCTCGTATTGAAGGTTGGGGAGTTGATTGGTGGAAGTCAGAGGGAGGAGCGATACGAGGTTATTCGTGACAGGTAGAAGAACAATCCATAGTTTTCTTGTTGGTTCTCATTTCTTTTGTGAGTCTGAATTCTCATTTCCTGTGCAGGATCAAGGAGATGGGTCTGCCACTTGAACCATACGAATGGTATCTTGATCTGAGACGATACGGGACAGTCAAGCACAGCGGTTTTGGTCTCGGGTTTGAGCGAATGCTTCTTTTTGCCACTGGCATTGACAACATCAGAGATGTTATCCCGTTCCCCAGATATCCAGGACGAGCAGACCTATGATATTCACAATACACAGATATGTGACAAACACAAGTTCTTAGGTTTCTTTACTATTCCGACAAAATCAAACATCTTTAAACGAGGATATTTAATTGTTTGTATTATATTGCCTTTCTTTTATTGTTGGGTTTTTATTTATTCTCGTAAATTTTGGATTGGTGGGTTTGTTTTTCTATAGCATAATATTGCATTTGAAATGACTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGAAGAGACTTTGATGGGTTTAGTGTCTTTGATAATTTGGTAGGTTCTAATTTGTGTATAGAAGAGAAGGTTGTACCAAGAATGTAAATTGAATATTTGAATATTTGACCTCTTCGTGAAAGATATGTCTTATCTAGGCAATAGGTCCAACTTTAGAAATTATATATTTGGTTGTTTTTTTTTTTTTTGTTTTTTTTTTGAGAAGTATTTGGGTTCTTTTTAATGCCTTTTTAGTTGCTTATTATAAAGAAATTATCATAGTTTATAAATTTGACGAAGAAAATAGAACATTTTGAAGGTGGGTTGGAAATTCTTTTTCTTTTTCATAGTCCTTGTAAATAACCTCACAGGAACAACTTTCCATAATTTTTGTTTCAGATTCTGACTGCTATTATATCATGAATTTAG

mRNA sequence

ATGGCAGAGCATTCCGCGCCACCCGCCGACCAACTCGCCGCCGTCGCCTTGAACGACGATGCTTCCACTTACAAATCTCTCAAAGCTGAATTCTCCGATCGCGTCCCGATCCGTTCAATCGTCTGCCGGCCTGACGGCGGCGCTGGACTCGCCGGCCAGAAGGTTCGCGTCGGCGGCTGGGTCAAAACTGGAAGGAAGGCCGACAAGGACGCTTTTGCCTTTCTCGAGTTGAACGATGGCTCTTGCCCTCAGAATCTCCAAGTGATCATCGAAGCGGCTGTGGCGGATCTTGGCCAGATTGTTCCGACTGGAACCTGCGTTGTTGTTGACGGCGTTTTCAAACTTCCTCCGCCGGATAAGCACCAGAAAGTCGAGCTTCGGGCTGAGGCTGTCGTCCATGTCGGTCCCGTTGATCCTGCGCGGTATCCTTTGCCGAAGACGAAACTCACTCTCGAGTTTCTCAGAGATTTCGTTCATCTCCGCCCTAGAACCAACACGATATCTGCGGTTGCTAGAATTCGAAATGCACTTGCATATGCCACTCATACATTTTTTAACAAACATGGATTTCTGTATATTCACACGCCAATTGTCACCACAAGCGATTGTGAGGGTGCGGGTGAAATGTTTCAAGTTACCACTCTATTGAGTGAGGCTGAAAGATTAGAGAAGGAGTTGATTAAAAACCCACCTCCATCAGAAGCAGAAGTAGATGAAGCAAGGAAAATTGTCAAGGAGAAAGGAGAAGCTGTTGCAAAGCTGAAGGCTTCTAAGGCGAGCAAGGAGGAAATTGGTGCCTCTGTTTTGGAACTGAAGAAAGCAAAGGAGAATGTTGCTCGGTTGGAAGAGAGAGCAAAGCTTAAACCAGGCATCCCAGAGAAAGATGGGAAAGTTGATTATGGACGGGATTTCTTTGCTCGGCAAGCGTTTTTGACTGTTTCTGGCCAGCTGCAAGTTGAAACTTATGCCTGTGCTCTTAGCAGTGTATACACATTTGGACCGACCTTCCGTGCCGAAAACTCTCACACTTCAAGACATCTTGCAGAGTTTTGGATGGTTGAGCCTGAAATTGCATTTGCAGATTTGCAGGATGATATGAATTGTGCGGAAGCATATGTGAGATTTCTGTGTCAGTGGTTACTTGACAACTGCTTAGATGACATGAAATTTATGGCTGACAAGTACGACCGAAGTTGTATTGATCGTCTCAAAATGGTCTCTTCAACCCCTTTCGTAAGAACTTCCTATACGGAAGCTGTTGAAATACTTGAGGAAGCCGTTAAGAAGGGTAAGCAGTTTGAGAACAAAGTGGAATGGGGAATTGATTTGGCTTCTGAGCATGAAAGATACTTGACTGAGGTGGTATTTAAAAAGCCTGTCATTGTCTACAACTATCCAAAGGGAATCAAAGCATTTTATATGAGGCTCAATGATGACTCAAAGACTGTAGCTGCAATGGATGTTCTTGTACCTAAGGTTGGGGAGTTGATTGGTGGAAGTCAGAGGGAGGAGCGATACGAGGTTATTCGTGACAGGATCAAGGAGATGGGTCTGCCACTTGAACCATACGAATGGTATCTTGATCTGAGACGATACGGGACAGTCAAGCACAGCGGTTTTGGTCTCGGGTTTGAGCGAATGCTTCTTTTTGCCACTGGCATTGACAACATCAGAGATGTTATCCCGTTCCCCAGATATCCAGGACGAGCAGACCTATGA

Coding sequence (CDS)

ATGGCAGAGCATTCCGCGCCACCCGCCGACCAACTCGCCGCCGTCGCCTTGAACGACGATGCTTCCACTTACAAATCTCTCAAAGCTGAATTCTCCGATCGCGTCCCGATCCGTTCAATCGTCTGCCGGCCTGACGGCGGCGCTGGACTCGCCGGCCAGAAGGTTCGCGTCGGCGGCTGGGTCAAAACTGGAAGGAAGGCCGACAAGGACGCTTTTGCCTTTCTCGAGTTGAACGATGGCTCTTGCCCTCAGAATCTCCAAGTGATCATCGAAGCGGCTGTGGCGGATCTTGGCCAGATTGTTCCGACTGGAACCTGCGTTGTTGTTGACGGCGTTTTCAAACTTCCTCCGCCGGATAAGCACCAGAAAGTCGAGCTTCGGGCTGAGGCTGTCGTCCATGTCGGTCCCGTTGATCCTGCGCGGTATCCTTTGCCGAAGACGAAACTCACTCTCGAGTTTCTCAGAGATTTCGTTCATCTCCGCCCTAGAACCAACACGATATCTGCGGTTGCTAGAATTCGAAATGCACTTGCATATGCCACTCATACATTTTTTAACAAACATGGATTTCTGTATATTCACACGCCAATTGTCACCACAAGCGATTGTGAGGGTGCGGGTGAAATGTTTCAAGTTACCACTCTATTGAGTGAGGCTGAAAGATTAGAGAAGGAGTTGATTAAAAACCCACCTCCATCAGAAGCAGAAGTAGATGAAGCAAGGAAAATTGTCAAGGAGAAAGGAGAAGCTGTTGCAAAGCTGAAGGCTTCTAAGGCGAGCAAGGAGGAAATTGGTGCCTCTGTTTTGGAACTGAAGAAAGCAAAGGAGAATGTTGCTCGGTTGGAAGAGAGAGCAAAGCTTAAACCAGGCATCCCAGAGAAAGATGGGAAAGTTGATTATGGACGGGATTTCTTTGCTCGGCAAGCGTTTTTGACTGTTTCTGGCCAGCTGCAAGTTGAAACTTATGCCTGTGCTCTTAGCAGTGTATACACATTTGGACCGACCTTCCGTGCCGAAAACTCTCACACTTCAAGACATCTTGCAGAGTTTTGGATGGTTGAGCCTGAAATTGCATTTGCAGATTTGCAGGATGATATGAATTGTGCGGAAGCATATGTGAGATTTCTGTGTCAGTGGTTACTTGACAACTGCTTAGATGACATGAAATTTATGGCTGACAAGTACGACCGAAGTTGTATTGATCGTCTCAAAATGGTCTCTTCAACCCCTTTCGTAAGAACTTCCTATACGGAAGCTGTTGAAATACTTGAGGAAGCCGTTAAGAAGGGTAAGCAGTTTGAGAACAAAGTGGAATGGGGAATTGATTTGGCTTCTGAGCATGAAAGATACTTGACTGAGGTGGTATTTAAAAAGCCTGTCATTGTCTACAACTATCCAAAGGGAATCAAAGCATTTTATATGAGGCTCAATGATGACTCAAAGACTGTAGCTGCAATGGATGTTCTTGTACCTAAGGTTGGGGAGTTGATTGGTGGAAGTCAGAGGGAGGAGCGATACGAGGTTATTCGTGACAGGATCAAGGAGATGGGTCTGCCACTTGAACCATACGAATGGTATCTTGATCTGAGACGATACGGGACAGTCAAGCACAGCGGTTTTGGTCTCGGGTTTGAGCGAATGCTTCTTTTTGCCACTGGCATTGACAACATCAGAGATGTTATCCCGTTCCCCAGATATCCAGGACGAGCAGACCTATGA

Protein sequence

MAEHSAPPADQLAAVALNDDASTYKSLKAEFSDRVPIRSIVCRPDGGAGLAGQKVRVGGWVKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDKHQKVELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEARKIVKEKGEAVAKLKASKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDYGRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRSCIDRLKMVSSTPFVRTSYTEAVEILEEAVKKGKQFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL

Homology

[Close]

BLAST of Spg002116 vs. NCBI nr
Match: XP_023531470.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1119.4 bits (2894), Expect = 0.0e+00
Identity = 555/572 (97.03%), Postives = 568/572 (99.30%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVALNDDASTYKSLKAEFSDRVPIRSIVCRPDGGAGLAGQKVRVGGW 60
           MAEHSAPP DQL+AVALNDDASTYKSLKAEFSDRVPIR+IVCRPDGG+GLAG KVRVGGW
Sbjct: 1   MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120

Query: 121 HQKVELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQK+ELRAEAVVHVGPVDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVAKLKASKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           RKIVKEKGEAV+ LKASKASK+EIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RKIVKEKGEAVSNLKASKASKQEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRSCIDRLKMVSSTPFVRTSYTEAV 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDR+CIDRLK+VSSTPFVRTSYTEA+
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420

Query: 421 EILEEAVKKGKQFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEA+KKGK+FENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREER+EVIRDRIKE+GLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Spg002116 vs. NCBI nr
Match: KAG6588323.1 (Asparagine--tRNA ligase, cytoplasmic 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020885.1 Asparagine--tRNA ligase, cytoplasmic 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1118.2 bits (2891), Expect = 0.0e+00
Identity = 554/572 (96.85%), Postives = 567/572 (99.13%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVALNDDASTYKSLKAEFSDRVPIRSIVCRPDGGAGLAGQKVRVGGW 60
           MAEHSAPP DQL+AVALNDD STYKSLKAEFSDRVPIR+IVCRPDGG+GLAG KVRVGGW
Sbjct: 1   MAEHSAPPIDQLSAVALNDDVSTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120

Query: 121 HQKVELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQK+ELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVAKLKASKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           RKIVKEKGEAV+ LKASKASK+EIGASV ELKKAKENVARLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRSCIDRLKMVSSTPFVRTSYTEAV 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDR+CIDRLK+VSSTPFVRTSYTEA+
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420

Query: 421 EILEEAVKKGKQFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEA+KKGK+FENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREER+EVIRDRIKE+GLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Spg002116 vs. NCBI nr
Match: XP_022932543.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita moschata])

HSP 1 Score: 1117.1 bits (2888), Expect = 0.0e+00
Identity = 554/572 (96.85%), Postives = 567/572 (99.13%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVALNDDASTYKSLKAEFSDRVPIRSIVCRPDGGAGLAGQKVRVGGW 60
           MAEHSAPP DQL+AVALNDDASTYKSLKAEFSDRVPIR+IVCRPDGG+GLAG KVRVGGW
Sbjct: 1   MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120

Query: 121 HQKVELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQK+ELRAEAVVHVGPVDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVAKLKASKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           RKIVKEKGEAV+ LKASKASK+EIGASV ELKKAKENVARLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRSCIDRLKMVSSTPFVRTSYTEAV 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDR+CIDRLK+VSSTPFVRTSYTEA+
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420

Query: 421 EILEEAVKKGKQFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEA+KKGK+FENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREER+EVIRDRIKE+GLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Spg002116 vs. NCBI nr
Match: XP_022965912.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita maxima])

HSP 1 Score: 1114.4 bits (2881), Expect = 0.0e+00
Identity = 553/572 (96.68%), Postives = 567/572 (99.13%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVALNDDASTYKSLKAEFSDRVPIRSIVCRPDGGAGLAGQKVRVGGW 60
           MAEHSAPP DQL+AV LNDDASTYKSLKAEFSDRVPIR+IVCRPDGG+GLAGQKVRVGGW
Sbjct: 1   MAEHSAPPIDQLSAVTLNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120

Query: 121 HQKVELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQK+ELRAEAVVHVG VDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKIELRAEAVVHVGLVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVAKLKASKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           RKIVKEKGEAV+ LKASKASK+EIGASVLELK+AKENVARLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RKIVKEKGEAVSNLKASKASKQEIGASVLELKQAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRSCIDRLKMVSSTPFVRTSYTEAV 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDR+CIDRLK+VSSTPFVRTSYTEA+
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420

Query: 421 EILEEAVKKGKQFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEA+KKGK+FENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREER+EVIRDRIKE+GLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Spg002116 vs. NCBI nr
Match: XP_022961000.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita moschata] >KAG7023917.1 Asparagine--tRNA ligase, cytoplasmic 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1105.5 bits (2858), Expect = 0.0e+00
Identity = 550/572 (96.15%), Postives = 564/572 (98.60%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVALNDDASTYKSLKAEFSDRVPIRSIVCRPDGGAGLAGQKVRVGGW 60
           MAEHSAPPADQLAAV LNDDA TY SLKAEFSDRVPIRSIV RPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEA++ADLGQIVPTGTCVVVDGVFKLPPP K
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRA+AVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL+KNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVAKLKASKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           R+IVKEKGEAVAKLKASKASKEEIGASV+ELKKAKENV++LEERAKLKPGIPEKDGKVDY
Sbjct: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
            +DFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRSCIDRLKMVSSTPFVRTSYTEAV 420
           DLQDDMNCAE+YVRFLCQWLLDNCLDDM+FMADKYDR+CIDRLK+VSSTPFVRTSYTEAV
Sbjct: 361 DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420

Query: 421 EILEEAVKKGKQFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAVKKGK FENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKE+ LPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Spg002116 vs. ExPASy Swiss-Prot
Match: Q9SW96 (Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 PE=1 SV=1)

HSP 1 Score: 862.4 bits (2227), Expect = 2.8e-249
Identity = 418/574 (72.82%), Postives = 496/574 (86.41%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVALNDDASTYKSLKAEFSDRVPIRSIVCRPDGGAGLAGQKVRVGGW 60
           MA+   PPA QLAAV+L +D ST +  +A+FS+RV IR+I+ RPDGGAGLAGQ VR+GGW
Sbjct: 1   MADEIVPPATQLAAVSLENDGSTVQ--RAQFSNRVLIRTILDRPDGGAGLAGQTVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VK+GR   K  F+FL +NDGSCP NLQV+++ ++ D+  +V TGTCV VDGV K+PP  K
Sbjct: 61  VKSGRDQGKRTFSFLAVNDGSCPANLQVMVDPSLYDVSNLVATGTCVTVDGVLKVPPKGK 120

Query: 121 --HQKVELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALA 180
              Q++EL    V+ VG VD ++YPLPKTKLTLE LRD +HLR RTN+ISAVARIRNALA
Sbjct: 121 GTQQQIELNVVKVIDVGTVDASKYPLPKTKLTLETLRDVLHLRSRTNSISAVARIRNALA 180

Query: 181 YATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVD 240
           +ATH+FF +H FLYIHTPI+TTSDCEGAGEMFQ TTL++  ERLE++LI NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQATTLINYTERLEQDLIDNPPPTEADVE 240

Query: 241 EARKIVKEKGEAVAKLKASKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKV 300
            AR IV E+G  VA+LKA+KASKE I A+V ELK AKE  A ++ER++L+PG+P+KDG +
Sbjct: 241 AARLIVIERGNVVAELKAAKASKEAITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNI 300

Query: 301 DYGRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360
           DY +DFF RQAFLTVSGQLQVETYACALS+VYTFGPTFRAENSHTSRHLAEFWMVEPEIA
Sbjct: 301 DYSKDFFGRQAFLTVSGQLQVETYACALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRSCIDRLKMVSSTPFVRTSYTE 420
           FADL+DDMNCAEAYV+++C WLL+ C  DM+ MA  +D  CIDRLK+V+STPF R +YT+
Sbjct: 361 FADLEDDMNCAEAYVKYMCNWLLEKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTK 420

Query: 421 AVEILEEAVKKGKQFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLND 480
           A+E+LEEAV KGK+F+N VEWGIDLASEHERYLTEV+F+KP+IVYNYPKGIKAFYMRLND
Sbjct: 421 AIELLEEAVAKGKEFDNNVEWGIDLASEHERYLTEVLFQKPLIVYNYPKGIKAFYMRLND 480

Query: 481 DSKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHS 540
           D KTVAAMDVLVPKVGELIGGSQREERY+VI+ RI+EMGLP+EPYEWYLDLRRYGTVKH 
Sbjct: 481 DEKTVAAMDVLVPKVGELIGGSQREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHC 540

Query: 541 GFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GFGLGFERM+LFATG+DNIRDVIPFPRYPG+ADL
Sbjct: 541 GFGLGFERMILFATGLDNIRDVIPFPRYPGKADL 572

[Close]

BLAST of Spg002116 vs. ExPASy Swiss-Prot
Match: Q9SSK1 (Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 PE=1 SV=1)

HSP 1 Score: 841.3 bits (2172), Expect = 6.6e-243
Identity = 420/576 (72.92%), Postives = 485/576 (84.20%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVALNDDASTYKSLKAEFSDRVPIRSIVCRPDGGAGLAGQKVRVGGW 60
           M +   PPA+QLAA  L +D ST +  KA+FSDRV IRSI+    GGA LAGQKVR+GGW
Sbjct: 1   MGDEIVPPANQLAADNLENDGSTVQ--KAQFSDRVLIRSIL---GGGAKLAGQKVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGR+  K  FAFLE+NDGSCP NLQV++++++ DL ++V TGTCV VDGV K+PP  K
Sbjct: 61  VKTGRQQGKGTFAFLEVNDGSCPANLQVMVDSSLYDLSRLVATGTCVTVDGVLKIPPEGK 120

Query: 121 --HQKVELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALA 180
              Q +EL  E V+ VG VDP  YPLPKTKLT EFLRD +HLR RTN ISAVARIRNALA
Sbjct: 121 GLKQSIELSVETVIAVGTVDPTTYPLPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALA 180

Query: 181 YATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVD 240
           +ATH+FF +H FLYIHTPI+TTSDCEGAGEMFQVTTL++  ER+E++LI NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQVTTLINHTERVEQDLIDNPPPTEADVE 240

Query: 241 EARKIVKEKGEAVAKLKASKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKV 300
            AR IVKE+GEAVA+LK +KASKEEI ASV +L  AK ++A +EER +LKPG+P+ DGK+
Sbjct: 241 AARLIVKERGEAVAQLKVAKASKEEITASVAQLSVAKASLAHVEERLRLKPGLPKNDGKI 300

Query: 301 DYGRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360
           DY  DFF RQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA
Sbjct: 301 DYSNDFFGRQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRSCIDRLKMVSSTPFVRTSYTE 420
           FAD+ DDMNCAEAYV+++C+WL+D C DDM+ M    D  C  RL MV+   F R +YTE
Sbjct: 361 FADIHDDMNCAEAYVKYMCKWLMDKCGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTE 420

Query: 421 AVEILEEAVKKGK-QFENKVEWGIDLASEHERYLTEVVF-KKPVIVYNYPKGIKAFYMRL 480
           A+E LE+AV +GK  F+NKVEWGIDLASEHERYLTEV F +KP+IVYNYPKGIKAFYMRL
Sbjct: 421 AIERLEKAVAQGKVVFDNKVEWGIDLASEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRL 480

Query: 481 NDDSKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVK 540
           NDD KTVAAMDVLVPKVGELIGGSQREERY+VI+ RI+EMGLP+EPYEWYLDLRRYGTVK
Sbjct: 481 NDDEKTVAAMDVLVPKVGELIGGSQREERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVK 540

Query: 541 HSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           H GFGLGFERM+ FATGIDNIRDVIPFPRYPG+ADL
Sbjct: 541 HCGFGLGFERMIQFATGIDNIRDVIPFPRYPGKADL 571

[Close]

BLAST of Spg002116 vs. ExPASy Swiss-Prot
Match: Q6MEC9 (Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=asnS PE=3 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 2.3e-142
Identity = 272/541 (50.28%), Postives = 339/541 (62.66%), Query Frame = 0


Query: 34  RVPIRSIVCRPDGGAGLAGQKVRVGGWVKTGRKADKDAFAFLELNDGSCPQNLQVIIEAA 93
           R  I+S+         L G  + + GWV+T R  ++  F F+E+NDGS   N Q+I    
Sbjct: 2   RTKIKSLRGTTPEVRALIGHDITLKGWVRTVR--NQKTFTFIEINDGSTLSNFQIIATPD 61

Query: 94  VADLGQIV---PTGTCVVVDGVFKLPPPDKHQKVELRAEAVVHVGPVDPARYPLPKTKLT 153
           +A   Q++    TG  V   G   +  P K Q +E++A A+  +G  DP  Y L K + T
Sbjct: 62  IAGYDQLINQLSTGVSVSAIGTI-VESPGKEQNLEMQATAITIIGKCDPEVYLLQKKRHT 121

Query: 154 LEFLRDFVHLRPRTNTISAVARIRNALAYATHTFFNKHGFLYIHTPIVTTSDCEGAGEMF 213
            EFLR   HLRPRTNTI AV R+RNALA+ATH FF K GFLYIHTPI+T SDCEGAG+MF
Sbjct: 122 FEFLRSIAHLRPRTNTIGAVTRVRNALAFATHQFFQKRGFLYIHTPIITGSDCEGAGKMF 181

Query: 214 QVTTLLSEAERLEKELIKNPPPSEAEVDEARKIVKEKGEAVAKLKASKASKEEIGASVLE 273
           QVTTL                                                       
Sbjct: 182 QVTTL------------------------------------------------------- 241

Query: 274 LKKAKENVARLEERAKLKPGIPEKDGKVDYGRDFFARQAFLTVSGQLQVETYACALSSVY 333
               + N AR  E            G+VDY +DFF +  +LTVSGQL  E YACALS VY
Sbjct: 242 ---DQNNPARTPE------------GRVDYTQDFFGKPTYLTVSGQLNGEIYACALSDVY 301

Query: 334 TFGPTFRAENSHTSRHLAEFWMVEPEIAFADLQDDMNCAEAYVRFLCQWLLDNCLDDMKF 393
           TFGPTFRAENS+TSRHLAEFWM+EPE+AFADL D+M+CAE Y++++ +++LDNC +DM+F
Sbjct: 302 TFGPTFRAENSNTSRHLAEFWMIEPEMAFADLNDNMDCAEDYLKYILKYVLDNCQEDMEF 361

Query: 394 MADKYDRSCIDRLKMVSSTPFVRTSYTEAVEILEEAVKKGKQFENKVEWGIDLASEHERY 453
                    I RL+ V +T F R SYT AV ILE+A KK   FE  V+WG+DL SEHER+
Sbjct: 362 FNKHVATDLISRLEHVINTSFERASYTYAVRILEKADKK---FEYPVKWGLDLQSEHERF 421

Query: 454 LTEVVFKKPVIVYNYPKGIKAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQREERYEVIR 513
           L E  F KPVI+ +YPK IKAFYMR N+D+KTVAAMDVLVPKVGE+IGGSQREER  V+ 
Sbjct: 422 LAEEFFGKPVILTDYPKDIKAFYMRTNEDNKTVAAMDVLVPKVGEIIGGSQREERLSVLE 466

Query: 514 DRIKEMGLPLEPYEWYLDLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRA 572
            ++KE  LP E Y WYL+LR++G+V HSGFG GFER++ F TG++NIRDVIPFPR+PG+A
Sbjct: 482 SKLKEFNLPAEEYWWYLELRKFGSVPHSGFGAGFERLVQFTTGMENIRDVIPFPRHPGKA 466

[Close]

BLAST of Spg002116 vs. ExPASy Swiss-Prot
Match: Q7MVE5 (Asparagine--tRNA ligase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83) OX=242619 GN=asnS PE=3 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 2.1e-140
Identity = 259/525 (49.33%), Postives = 328/525 (62.48%), Query Frame = 0


Query: 49  GLAGQKVRVGGWVKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQI--VPTGTC 108
           GL GQ++ + GWV+T  K    A  F+ LNDGS   N+Q++ + A  D  Q+  + TG C
Sbjct: 17  GLVGQEINIKGWVRT--KRGNKAVNFIALNDGSTIHNMQIVADLASFDAAQMSQITTGAC 76

Query: 109 VVVDGVFKLPPPDKHQKVELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNT 168
           + V G   +      Q VE++A A+   G  DPA YPL K   TLEFLR+  HLRPRTNT
Sbjct: 77  IGVIGTL-VESQGAGQSVEVQASAIEIYGTADPATYPLQKKGHTLEFLREIAHLRPRTNT 136

Query: 169 ISAVARIRNALAYATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL 228
             A+ RIR+ +A A HTFF+  G+ Y H P++T SDCEGAG+MFQVTTL           
Sbjct: 137 FGAIYRIRHHMAIAIHTFFHNKGYYYFHAPLITASDCEGAGQMFQVTTL----------- 196

Query: 229 IKNPPPSEAEVDEARKIVKEKGEAVAKLKASKASKEEIGASVLELKKAKENVARLEERAK 288
             NP                                             +++ R EE   
Sbjct: 197 --NP---------------------------------------------DSLPRTEE--- 256

Query: 289 LKPGIPEKDGKVDYGRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRH 348
                    G+VDY +DFF R   LTVSGQL+ E  A AL  +YTFGPTFRAENS+T RH
Sbjct: 257 ---------GQVDYRKDFFGRHTSLTVSGQLEGEMAAMALGGIYTFGPTFRAENSNTPRH 316

Query: 349 LAEFWMVEPEIAFADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRSCIDRLKMV 408
           LAEFWM+EPE+AF +++D+M+ AE ++++  QW LDNC+DD+ F+++ +D+  IDRLK V
Sbjct: 317 LAEFWMIEPEVAFLEIEDNMDLAEEFIKYCVQWALDNCMDDISFLSEHFDKELIDRLKFV 376

Query: 409 SSTPFVRTSYTEAVEILEEAVKKGKQFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYP 468
              PFVR +YTE + ILEEAVK G +FE  + WG DLASEHERYL EV FK PVI+ +YP
Sbjct: 377 LEKPFVRLAYTEGIRILEEAVKNGVKFEFPIYWGADLASEHERYLVEVHFKTPVIMTDYP 436

Query: 469 KGIKAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWY 528
           K IK+FYM+LNDD KTV  MDVL PK+GE+IGGS+RE  Y+ +  R  EMG+P +   WY
Sbjct: 437 KEIKSFYMKLNDDGKTVRGMDVLFPKIGEIIGGSEREADYDKLVARANEMGVPEKDIWWY 468

Query: 529 LDLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRAD 572
           LD RRYGT  HSGFGLGFER+LLF TG+ NIRDVIPFPR P  A+
Sbjct: 497 LDSRRYGTAPHSGFGLGFERLLLFVTGMSNIRDVIPFPRTPNNAE 468

[Close]

BLAST of Spg002116 vs. ExPASy Swiss-Prot
Match: B2RK42 (Asparagine--tRNA ligase OS=Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / CIP 103683 / JCM 12257 / NCTC 11834 / 2561) OX=431947 GN=asnS PE=3 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 6.2e-140
Identity = 258/525 (49.14%), Postives = 327/525 (62.29%), Query Frame = 0


Query: 49  GLAGQKVRVGGWVKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQI--VPTGTC 108
           GL GQ++ + GWV+T  K    A  F+ LNDGS   N+Q++ + A  D  Q+  + TG C
Sbjct: 17  GLVGQEINIKGWVRT--KRGNKAVNFIALNDGSTIHNMQIVADLASFDAAQMSQITTGAC 76

Query: 109 VVVDGVFKLPPPDKHQKVELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNT 168
           + V G   +      Q VE++A A+   G  DPA YPL K   TLEFLR+  HLRPRTNT
Sbjct: 77  IGVIGTL-VESQGAGQSVEVQASAIEIYGTADPATYPLQKKGHTLEFLREIAHLRPRTNT 136

Query: 169 ISAVARIRNALAYATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL 228
              + RIR+ +A A HTFF+  G+ Y H P++T SDCEGAG+MFQVTTL           
Sbjct: 137 FGVIYRIRHHMAIAIHTFFHNKGYYYFHAPLITASDCEGAGQMFQVTTL----------- 196

Query: 229 IKNPPPSEAEVDEARKIVKEKGEAVAKLKASKASKEEIGASVLELKKAKENVARLEERAK 288
             NP                                             +++ R EE   
Sbjct: 197 --NP---------------------------------------------DSLPRTEE--- 256

Query: 289 LKPGIPEKDGKVDYGRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRH 348
                    G+VDY +DFF R   LTVSGQL+ E  A AL  +YTFGPTFRAENS+T RH
Sbjct: 257 ---------GQVDYRKDFFGRHTSLTVSGQLEGEMAAMALGGIYTFGPTFRAENSNTPRH 316

Query: 349 LAEFWMVEPEIAFADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRSCIDRLKMV 408
           LAEFWM+EPE+AF +++D+M+ AE ++++  QW LDNC+DD+ F+++ +D+  IDRLK V
Sbjct: 317 LAEFWMIEPEVAFLEIEDNMDLAEEFIKYCVQWALDNCMDDISFLSEHFDKELIDRLKFV 376

Query: 409 SSTPFVRTSYTEAVEILEEAVKKGKQFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYP 468
              PFVR +YTE + ILEEAVK G +FE  + WG DLASEHERYL EV FK PVI+ +YP
Sbjct: 377 LEKPFVRLAYTEGIRILEEAVKNGVKFEFPIYWGADLASEHERYLVEVHFKTPVIMTDYP 436

Query: 469 KGIKAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWY 528
           K IK+FYM+LNDD KTV  MDVL PK+GE+IGGS+RE  Y+ +  R  EMG+P +   WY
Sbjct: 437 KEIKSFYMKLNDDGKTVRGMDVLFPKIGEIIGGSEREADYDKLVARANEMGVPEKDIWWY 468

Query: 529 LDLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRAD 572
           LD RRYGT  HSGFGLGFER+LLF TG+ NIRDVIPFPR P  A+
Sbjct: 497 LDSRRYGTAPHSGFGLGFERLLLFVTGMSNIRDVIPFPRTPNNAE 468

[Close]

BLAST of Spg002116 vs. ExPASy TrEMBL
Match: A0A6J1F212 (Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111439035 PE=3 SV=1)

HSP 1 Score: 1117.1 bits (2888), Expect = 0.0e+00
Identity = 554/572 (96.85%), Postives = 567/572 (99.13%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVALNDDASTYKSLKAEFSDRVPIRSIVCRPDGGAGLAGQKVRVGGW 60
           MAEHSAPP DQL+AVALNDDASTYKSLKAEFSDRVPIR+IVCRPDGG+GLAG KVRVGGW
Sbjct: 1   MAEHSAPPIDQLSAVALNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGHKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120

Query: 121 HQKVELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQK+ELRAEAVVHVGPVDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKIELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVAKLKASKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           RKIVKEKGEAV+ LKASKASK+EIGASV ELKKAKENVARLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RKIVKEKGEAVSNLKASKASKQEIGASVSELKKAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRSCIDRLKMVSSTPFVRTSYTEAV 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDR+CIDRLK+VSSTPFVRTSYTEA+
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420

Query: 421 EILEEAVKKGKQFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEA+KKGK+FENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREER+EVIRDRIKE+GLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Spg002116 vs. ExPASy TrEMBL
Match: A0A6J1HLL2 (Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111465653 PE=3 SV=1)

HSP 1 Score: 1114.4 bits (2881), Expect = 0.0e+00
Identity = 553/572 (96.68%), Postives = 567/572 (99.13%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVALNDDASTYKSLKAEFSDRVPIRSIVCRPDGGAGLAGQKVRVGGW 60
           MAEHSAPP DQL+AV LNDDASTYKSLKAEFSDRVPIR+IVCRPDGG+GLAGQKVRVGGW
Sbjct: 1   MAEHSAPPIDQLSAVTLNDDASTYKSLKAEFSDRVPIRTIVCRPDGGSGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120

Query: 121 HQKVELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQK+ELRAEAVVHVG VDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKIELRAEAVVHVGLVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVAKLKASKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           RKIVKEKGEAV+ LKASKASK+EIGASVLELK+AKENVARLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RKIVKEKGEAVSNLKASKASKQEIGASVLELKQAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRSCIDRLKMVSSTPFVRTSYTEAV 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDR+CIDRLK+VSSTPFVRTSYTEA+
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKLVSSTPFVRTSYTEAI 420

Query: 421 EILEEAVKKGKQFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEA+KKGK+FENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAIKKGKEFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREER+EVIRDRIKE+GLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERFEVIRDRIKEVGLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Spg002116 vs. ExPASy TrEMBL
Match: A0A6J1HAP9 (Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111461633 PE=3 SV=1)

HSP 1 Score: 1105.5 bits (2858), Expect = 0.0e+00
Identity = 550/572 (96.15%), Postives = 564/572 (98.60%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVALNDDASTYKSLKAEFSDRVPIRSIVCRPDGGAGLAGQKVRVGGW 60
           MAEHSAPPADQLAAV LNDDA TY SLKAEFSDRVPIRSIV RPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEA++ADLGQIVPTGTCVVVDGVFKLPPP K
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRA+AVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL+KNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVAKLKASKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           R+IVKEKGEAVAKLKASKASKEEIGASV+ELKKAKENV++LEERAKLKPGIPEKDGKVDY
Sbjct: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
            +DFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRSCIDRLKMVSSTPFVRTSYTEAV 420
           DLQDDMNCAE+YVRFLCQWLLDNCLDDM+FMADKYDR+CIDRLK+VSSTPFVRTSYTEAV
Sbjct: 361 DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420

Query: 421 EILEEAVKKGKQFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAVKKGK FENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKE+ LPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Spg002116 vs. ExPASy TrEMBL
Match: A0A6J1JKK1 (Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111485413 PE=3 SV=1)

HSP 1 Score: 1103.6 bits (2853), Expect = 0.0e+00
Identity = 550/572 (96.15%), Postives = 563/572 (98.43%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVALNDDASTYKSLKAEFSDRVPIRSIVCRPDGGAGLAGQKVRVGGW 60
           MAEHSAPPADQLAAV LNDDA TY SLKAEFSDRVPIRSIV RPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEA++ADLGQIVPTGTCVVVDGVFKLPPP K
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRA+AVVHVG VDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL+KNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240

Query: 241 RKIVKEKGEAVAKLKASKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           R+IVKEKGEAVAKLKASKASKEEIGASV+ELKKAKENV++LEERAKLKPGIPEKDGKVDY
Sbjct: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
            +DFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRSCIDRLKMVSSTPFVRTSYTEAV 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDM+FMADKYDR+CIDRLK+VSSTPFVRTSYTEAV
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420

Query: 421 EILEEAVKKGKQFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAVKKGK FENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKE+ LPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Spg002116 vs. ExPASy TrEMBL
Match: A0A6J1DMU4 (Asparagine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111022537 PE=3 SV=1)

HSP 1 Score: 1097.8 bits (2838), Expect = 0.0e+00
Identity = 550/572 (96.15%), Postives = 559/572 (97.73%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVALNDDASTYKSLKAEFSDRVPIRSIVCRPDGGAGLAGQKVRVGGW 60
           MAE+SAPPADQLAA  LNDDA TYK+LKAEFSDRVPIRSIV RPDGGAGLAGQKVRVGGW
Sbjct: 1   MAENSAPPADQLAAATLNDDAPTYKALKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPP K
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
            QKVELRAEAVVHVGPVDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 QQKVELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAE+LEKELIKNPPPSEA+V+ A
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAEKLEKELIKNPPPSEADVEAA 240

Query: 241 RKIVKEKGEAVAKLKASKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300
           RKIVKEKGE VAKLKA+KASKEEIGASVLELKKAKENVARLEER+KLKPGIPEKDGKVDY
Sbjct: 241 RKIVKEKGEDVAKLKAAKASKEEIGASVLELKKAKENVARLEERSKLKPGIPEKDGKVDY 300

Query: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRSCIDRLKMVSSTPFVRTSYTEAV 420
           DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDR+CIDRLKMVSSTPFVR SYTEAV
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKMVSSTPFVRISYTEAV 420

Query: 421 EILEEAVKKGKQFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAVKKGK FENKVEWGIDLASEHERYLTEV FKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVEFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGEL+GGSQREERYEVIRDRIKEM LPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELVGGSQREERYEVIRDRIKEMDLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Spg002116 vs. TAIR 10
Match: AT5G56680.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 862.4 bits (2227), Expect = 2.0e-250
Identity = 418/574 (72.82%), Postives = 496/574 (86.41%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVALNDDASTYKSLKAEFSDRVPIRSIVCRPDGGAGLAGQKVRVGGW 60
           MA+   PPA QLAAV+L +D ST +  +A+FS+RV IR+I+ RPDGGAGLAGQ VR+GGW
Sbjct: 1   MADEIVPPATQLAAVSLENDGSTVQ--RAQFSNRVLIRTILDRPDGGAGLAGQTVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VK+GR   K  F+FL +NDGSCP NLQV+++ ++ D+  +V TGTCV VDGV K+PP  K
Sbjct: 61  VKSGRDQGKRTFSFLAVNDGSCPANLQVMVDPSLYDVSNLVATGTCVTVDGVLKVPPKGK 120

Query: 121 --HQKVELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALA 180
              Q++EL    V+ VG VD ++YPLPKTKLTLE LRD +HLR RTN+ISAVARIRNALA
Sbjct: 121 GTQQQIELNVVKVIDVGTVDASKYPLPKTKLTLETLRDVLHLRSRTNSISAVARIRNALA 180

Query: 181 YATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVD 240
           +ATH+FF +H FLYIHTPI+TTSDCEGAGEMFQ TTL++  ERLE++LI NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQATTLINYTERLEQDLIDNPPPTEADVE 240

Query: 241 EARKIVKEKGEAVAKLKASKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKV 300
            AR IV E+G  VA+LKA+KASKE I A+V ELK AKE  A ++ER++L+PG+P+KDG +
Sbjct: 241 AARLIVIERGNVVAELKAAKASKEAITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNI 300

Query: 301 DYGRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360
           DY +DFF RQAFLTVSGQLQVETYACALS+VYTFGPTFRAENSHTSRHLAEFWMVEPEIA
Sbjct: 301 DYSKDFFGRQAFLTVSGQLQVETYACALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRSCIDRLKMVSSTPFVRTSYTE 420
           FADL+DDMNCAEAYV+++C WLL+ C  DM+ MA  +D  CIDRLK+V+STPF R +YT+
Sbjct: 361 FADLEDDMNCAEAYVKYMCNWLLEKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTK 420

Query: 421 AVEILEEAVKKGKQFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLND 480
           A+E+LEEAV KGK+F+N VEWGIDLASEHERYLTEV+F+KP+IVYNYPKGIKAFYMRLND
Sbjct: 421 AIELLEEAVAKGKEFDNNVEWGIDLASEHERYLTEVLFQKPLIVYNYPKGIKAFYMRLND 480

Query: 481 DSKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHS 540
           D KTVAAMDVLVPKVGELIGGSQREERY+VI+ RI+EMGLP+EPYEWYLDLRRYGTVKH 
Sbjct: 481 DEKTVAAMDVLVPKVGELIGGSQREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHC 540

Query: 541 GFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GFGLGFERM+LFATG+DNIRDVIPFPRYPG+ADL
Sbjct: 541 GFGLGFERMILFATGLDNIRDVIPFPRYPGKADL 572

[Close]

BLAST of Spg002116 vs. TAIR 10
Match: AT1G70980.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 841.3 bits (2172), Expect = 4.7e-244
Identity = 420/576 (72.92%), Postives = 485/576 (84.20%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVALNDDASTYKSLKAEFSDRVPIRSIVCRPDGGAGLAGQKVRVGGW 60
           M +   PPA+QLAA  L +D ST +  KA+FSDRV IRSI+    GGA LAGQKVR+GGW
Sbjct: 1   MGDEIVPPANQLAADNLENDGSTVQ--KAQFSDRVLIRSIL---GGGAKLAGQKVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPDK 120
           VKTGR+  K  FAFLE+NDGSCP NLQV++++++ DL ++V TGTCV VDGV K+PP  K
Sbjct: 61  VKTGRQQGKGTFAFLEVNDGSCPANLQVMVDSSLYDLSRLVATGTCVTVDGVLKIPPEGK 120

Query: 121 --HQKVELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALA 180
              Q +EL  E V+ VG VDP  YPLPKTKLT EFLRD +HLR RTN ISAVARIRNALA
Sbjct: 121 GLKQSIELSVETVIAVGTVDPTTYPLPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALA 180

Query: 181 YATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVD 240
           +ATH+FF +H FLYIHTPI+TTSDCEGAGEMFQVTTL++  ER+E++LI NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQVTTLINHTERVEQDLIDNPPPTEADVE 240

Query: 241 EARKIVKEKGEAVAKLKASKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKV 300
            AR IVKE+GEAVA+LK +KASKEEI ASV +L  AK ++A +EER +LKPG+P+ DGK+
Sbjct: 241 AARLIVKERGEAVAQLKVAKASKEEITASVAQLSVAKASLAHVEERLRLKPGLPKNDGKI 300

Query: 301 DYGRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360
           DY  DFF RQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA
Sbjct: 301 DYSNDFFGRQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRSCIDRLKMVSSTPFVRTSYTE 420
           FAD+ DDMNCAEAYV+++C+WL+D C DDM+ M    D  C  RL MV+   F R +YTE
Sbjct: 361 FADIHDDMNCAEAYVKYMCKWLMDKCGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTE 420

Query: 421 AVEILEEAVKKGK-QFENKVEWGIDLASEHERYLTEVVF-KKPVIVYNYPKGIKAFYMRL 480
           A+E LE+AV +GK  F+NKVEWGIDLASEHERYLTEV F +KP+IVYNYPKGIKAFYMRL
Sbjct: 421 AIERLEKAVAQGKVVFDNKVEWGIDLASEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRL 480

Query: 481 NDDSKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVK 540
           NDD KTVAAMDVLVPKVGELIGGSQREERY+VI+ RI+EMGLP+EPYEWYLDLRRYGTVK
Sbjct: 481 NDDEKTVAAMDVLVPKVGELIGGSQREERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVK 540

Query: 541 HSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           H GFGLGFERM+ FATGIDNIRDVIPFPRYPG+ADL
Sbjct: 541 HCGFGLGFERMIQFATGIDNIRDVIPFPRYPGKADL 571

[Close]

BLAST of Spg002116 vs. TAIR 10
Match: AT4G17300.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 488.8 bits (1257), Expect = 5.9e-138
Identity = 266/553 (48.10%), Postives = 344/553 (62.21%), Query Frame = 0


Query: 24  YKSLKAEFSDRVPIRSIVCRPDGGAGLAGQKVRVGGWVKTGRKADKDAFAFLELNDGSCP 83
           + S   EF  ++ I  +    D G    GQ + + GWV+T R   + +  F+E+NDGSC 
Sbjct: 81  FGSKVGEFRKKLRIAEVKGGADEGLSRVGQSLNIMGWVRTLR--SQSSVTFIEINDGSCL 140

Query: 84  QNLQVIIEAAVADLGQI----VPTGTCVVVDGVFKLPPPDKHQKVELRAEAVVHVGPVDP 143
            NLQ ++ +      Q+    + TG  V V G        K QKVEL+ E ++ VG  D 
Sbjct: 141 SNLQCVMTSDAEGYDQVESGSILTGASVSVQGTIVASQGTK-QKVELKVEKIIVVGECD- 200

Query: 144 ARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYATHTFFNKHGFLYIHTPIVT 203
           + YP+ K +++ EFLR   HLRPRTNT  AVAR+RN LAYATH FF + GF+++ +PI+T
Sbjct: 201 SSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNTLAYATHKFFQESGFVWVASPIIT 260

Query: 204 TSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEARKIVKEKGEAVAKLKASKA 263
            SDCEGAGE F VTTL+                                          +
Sbjct: 261 ASDCEGAGEQFCVTTLI-----------------------------------------PS 320

Query: 264 SKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDYGRDFFARQAFLTVSGQLQV 323
           S E    S+  + K                    K G +D+ +DFF + AFLTVSGQL  
Sbjct: 321 SHENTDTSIDAIPKT-------------------KGGLIDWSQDFFGKPAFLTVSGQLNG 380

Query: 324 ETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLQDDMNCAEAYVRFLCQW 383
           ETYA ALS VYTFGPTFRAENS+TSRHLAEFWM+EPE+AFADL DDM CA AY++++ ++
Sbjct: 381 ETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMACATAYLQYVVKY 440

Query: 384 LLDNCLDDMKFMADKYDRSCIDRLKMVSSTPFVRTSYTEAVEILEEAVKKGKQFENKVEW 443
           +LDNC +DM+F     ++  I RL  V+   F++  YT+A+EIL   +K  K+F+  V+W
Sbjct: 441 VLDNCKEDMEFFDTWIEKGIIRRLSDVAEKEFLQLGYTDAIEIL---LKANKKFDFPVKW 500

Query: 444 GIDLASEHERYLTEVVF-KKPVIVYNYPKGIKAFYMRLNDDSKTVAAMDVLVPKVGELIG 503
           G+DL SEHERY+TE  F  +PVI+ +YPK IKAFYMR NDD KTVAAMD+LVP++GELIG
Sbjct: 501 GLDLQSEHERYITEEAFGGRPVIIRDYPKEIKAFYMRENDDGKTVAAMDMLVPRIGELIG 560

Query: 504 GSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHSGFGLGFERMLLFATGIDNIR 563
           GSQREER EV+  R+ E+ L  E Y WYLDLRRYG+V H+GFGLGFER++ F TGIDNIR
Sbjct: 561 GSQREERLEVLEARLDELKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQFVTGIDNIR 566

Query: 564 DVIPFPRYPGRAD 572
           DVIPFPR P  A+
Sbjct: 621 DVIPFPRTPASAE 566

[Close]

BLAST of Spg002116 vs. TAIR 10
Match: AT3G07420.1 (asparaginyl-tRNA synthetase 2 )

HSP 1 Score: 478.4 bits (1230), Expect = 8.0e-135
Identity = 260/633 (41.07%), Postives = 381/633 (60.19%), Query Frame = 0


Query: 18  NDDASTYKSLKAEFSDRVPIRSIVCRPDGGAGLAGQKVRVGGWVKTGRKADKDA------ 77
           ++D S      +++S RV +++++ R D GAGLAG++V +GGWVK+ R   K++      
Sbjct: 13  DNDLSPKPITLSKYSKRVELKTLLDRSDRGAGLAGKRVVIGGWVKSARAVKKNSPPPPLP 72

Query: 78  ------------------------------------------------------------ 137
                                                                       
Sbjct: 73  VVAAPSPSSGGDQAHTTANIRCTEIIQSKMNIFKRFFDVLSGGGKTYPIFDKTELAGQKA 132

Query: 138 -------FAFLELNDGSCPQNLQVIIEAAVADL--GQIVPTGTCVVVDGVFKLP-PPDKH 197
                  F FL ++DGS   +LQV++++A++ +   Q++  GTC+V +GV +LP      
Sbjct: 133 VPPPEYVFYFL-ISDGSSISSLQVVVDSALSTVPATQLMALGTCIVAEGVLRLPLAASAK 192

Query: 198 QKVELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYAT 257
             +EL AE ++HVG VDP +YPL K +L L  LRDF H RPRT T+ +V R+ +AL  A+
Sbjct: 193 HVIELEAEKLLHVGTVDPEKYPLSKKQLPLHMLRDFSHFRPRTTTVGSVTRVHSALTLAS 252

Query: 258 HTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAE---VD 317
           HTF   HGF Y+  P++TT+   G GEMF+VTTLL + +  E+   K PP  E +   +D
Sbjct: 253 HTFLQYHGFQYVQVPVITTT--TGFGEMFRVTTLLGKTDDKEE---KKPPVQEKDGFSID 312

Query: 318 EARKIVKEKGEAVAKLKASKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKV 377
             + ++KEK   +  LK S +++E + A+V +LKK  +  +++E + K K G   K  K+
Sbjct: 313 TVKAVIKEKTRLIDHLKRSDSNRETVVAAVHDLKKTNDLASQIEMKQKSKTGTLVKPEKL 372

Query: 378 DYGRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 437
           D+ +DFF R  +LT SG+  +E+YA AL  VYTFGP F A+    +RHLAE W VE E+A
Sbjct: 373 DFSKDFFGRDTYLTASGRFHLESYASALGKVYTFGPRFIADKIDNARHLAEKWNVETEMA 432

Query: 438 FADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRSCIDRLKMVSSTPFVRTSYTE 497
           FA+L D M+CA+ Y +FLC+++L+N  +DMKF++ + D++   RL+  +S+  +R SYTE
Sbjct: 433 FAELDDAMDCADEYFKFLCKYVLENRDEDMKFISKRVDKTITTRLEATASSSLLRFSYTE 492

Query: 498 AVEILEEAVKKGKQFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLND 557
            + +L++A     +FE K EWG+ L +EH  YLT+ ++K PVIV+ YPK IK FY+RLND
Sbjct: 493 VISLLQKATT--TKFETKPEWGVALTTEHLSYLTDEIYKGPVIVHTYPKAIKQFYVRLND 552

Query: 558 DSKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHS 572
           D KTVAA D++VPKVG +I GSQ EER+E++  RI E G   E +EWYLDLRR+GTVKHS
Sbjct: 553 DKKTVAAFDLVVPKVGVVITGSQNEERFEILDARIGESGFTREKFEWYLDLRRHGTVKHS 612

[Close]

BLAST of Spg002116 vs. TAIR 10
Match: AT4G31180.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 118.2 bits (295), Expect = 2.1e-26
Identity = 90/291 (30.93%), Postives = 145/291 (49.83%), Query Frame = 0


Query: 286 KLKPGIPEKDGKVDYGRDFFARQAFLTVSGQLQVETYACA-LSSVYTFGPTFRAENSHTS 345
           KL  G  E  G   +  ++  + A L  S QL  +   C  L  V+  GP FRAE+S T 
Sbjct: 279 KLMAGSSE-GGSAVFRLEYKGQPACLAQSPQLHKQMAICGDLRRVFEVGPVFRAEDSFTH 338

Query: 346 RHLAEFWMVEPEIAF-ADLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRSCIDRL 405
           RHL EF  ++ E+       + M+  +    F+   L + C  +++ +  +Y     + L
Sbjct: 339 RHLCEFVGLDVEMEIRKHYSEIMDLVDELFVFIFTSLNERCKKELQAVGKQYP---FEPL 398

Query: 406 KMVSSTPFVRTSYTEAVEILEEAVKKGKQFENKVEWGIDLASEHERYLTEVVFKKP---- 465
           K +  T  +R ++ E V++L+EA         +V+   DL +E ER L ++V +K     
Sbjct: 399 KFLPKT--LRLTFEEGVQMLKEA-------GVEVDPLGDLNTESERKLGQLVLEKYNTEF 458

Query: 466 VIVYNYPKGIKAFY-MRLNDDSKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGL 525
            I++ YPK ++ FY M   D+     + DV + +  E+I G+QR    EV+  R  E G+
Sbjct: 459 YILHRYPKAVRPFYTMTCADNPLYSNSFDVFI-RGEEIISGAQRVHIPEVLEQRAGECGI 518

Query: 526 PLEPYEWYLDLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGR 570
            ++    Y+D  RYG   H GFG+G ER+++    ++NIR    FPR P R
Sbjct: 519 DVKTISTYIDSFRYGAPLHGGFGVGLERVVMLFCALNNIRKTSLFPRDPQR 555

The following BLAST results are available for this feature:

BLAST of Spg002116 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023531470.1	0.0e+00	97.03	asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita pepo subsp. pepo]	[more]
KAG6588323.1	0.0e+00	96.85	Asparagine--tRNA ligase, cytoplasmic 1, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_022932543.1	0.0e+00	96.85	asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita moschata]	[more]
XP_022965912.1	0.0e+00	96.68	asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita maxima]	[more]
XP_022961000.1	0.0e+00	96.15	asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita moschata] >KAG7023917.1 A...	[more]

BLAST of Spg002116 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SW96	2.8e-249	72.82	Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 ...	[more]
Q9SSK1	6.6e-243	72.92	Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 ...	[more]
Q6MEC9	2.3e-142	50.28	Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 G...	[more]
Q7MVE5	2.1e-140	49.33	Asparagine--tRNA ligase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83) ...	[more]
B2RK42	6.2e-140	49.14	Asparagine--tRNA ligase OS=Porphyromonas gingivalis (strain ATCC 33277 / DSM 207...	[more]

BLAST of Spg002116 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F212	0.0e+00	96.85	Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111439035 PE=3 SV=1	[more]
A0A6J1HLL2	0.0e+00	96.68	Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111465653 PE=3 SV=1	[more]
A0A6J1HAP9	0.0e+00	96.15	Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111461633 PE=3 SV=1	[more]
A0A6J1JKK1	0.0e+00	96.15	Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111485413 PE=3 SV=1	[more]
A0A6J1DMU4	0.0e+00	96.15	Asparagine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111022537 PE=3 SV=1	[more]

BLAST of Spg002116 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G56680.1	2.0e-250	72.82	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT1G70980.1	4.7e-244	72.92	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT4G17300.1	5.9e-138	48.10	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT3G07420.1	8.0e-135	41.07	asparaginyl-tRNA synthetase 2	[more]
AT4G31180.1	2.1e-26	30.93	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 264..284
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 143..571 e-value: 5.1E-108 score: 363.4
None	No IPR available	GENE3D	2.40.50.140		coord: 34..138 e-value: 1.0E-9 score: 40.1
None	No IPR available	PANTHER	PTHR22594	ASPARTYL/LYSYL-TRNA SYNTHETASE	coord: 1..572
None	No IPR available	PANTHER	PTHR22594:SF52	BNAC03G13340D PROTEIN	coord: 1..572
None	No IPR available	CDD	cd00776	AsxRS_core	coord: 148..568 e-value: 2.61548E-143 score: 415.041
None	No IPR available	CDD	cd04318	EcAsnRS_like_N	coord: 54..135 e-value: 4.05107E-25 score: 96.8656
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 151..567
IPR002312	Aspartyl/Asparaginyl-tRNA synthetase, class IIb	PRINTS	PR01042	TRNASYNTHASP	coord: 327..340 score: 43.83 coord: 487..503 score: 44.79 coord: 311..323 score: 40.73 coord: 527..541 score: 47.27
IPR004364	Aminoacyl-tRNA synthetase, class II (D/K/N)	PFAM	PF00152	tRNA-synt_2	coord: 152..214 e-value: 3.5E-12 score: 46.0
IPR004364	Aminoacyl-tRNA synthetase, class II (D/K/N)	PFAM	PF00152	tRNA-synt_2	coord: 303..565 e-value: 4.7E-63 score: 213.2
IPR004522	Asparagine-tRNA ligase	TIGRFAM	TIGR00457	TIGR00457	coord: 51..571 e-value: 1.3E-167 score: 556.1
IPR004522	Asparagine-tRNA ligase	HAMAP	MF_00534	Asn_tRNA_synth	coord: 37..572 score: 42.14632
IPR006195	Aminoacyl-tRNA synthetase, class II	PROSITE	PS50862	AA_TRNA_LIGASE_II	coord: 329..562 score: 13.63026
IPR000738	WHEP-TRS domain	PROSITE	PS51185	WHEP_TRS_2	coord: 236..292 score: 10.258863
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 47..135

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg002116.1	Spg002116.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006421	asparaginyl-tRNA aminoacylation
biological_process	GO:0006418	tRNA aminoacylation for protein translation
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0004816	asparagine-tRNA ligase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding