Spg001648 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg001648
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	RNase H domain-containing protein
Location	scaffold10: 348960 .. 350026 (+)
RNA-Seq Expression	Spg001648
Synteny	Spg001648

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCGCCGGGACTCAACTCACCCGCCTTATCGTCATCCCTGACCCTGTGGCGGATGTTGTTGGACTTTATCCAAAATTACTCCATATTGAGCCGACCCACCCCTCCAGAATATATTAGGCTGGGACTTTAGAATCTGAATTCATTTGAATGTTGATGTCTGTGTTTGGTGTGTTGTATTTAGTCTGGATTTAATTTGATGGAGTGCAAGATTGGATTTGGGTTGGAATCGTATTGGTTGTGTTTTTTTTTTTTTTTTGTAAATATACTCACTATTTTTCCGCTTATTTAAGACGGAGTTGTTTCCCTTCGGAGGACGTATTACTCTATTCAAATTATTTGTGTTCGCTCTTTCTTTTCTCTAAATTTTGTTGGCAAATATGGATGTTCAGAAACAACATATACCACAATAACCAGATTGGAGAAGAGCATTGAAGACTACCTATCGGAATTTGGCAAAGCAGGAAGAACGAACTTGATTTCCCAGCGTCAACCCGACAGTGACCATTCCTGCAGCGGCGGCTTCAAGGACGAACTTGAACCCCCAGCTTCTATGGTTGTCGATCCCTACCAGCATATGGAAGCTTAGCTGCGACGCGTCCTGGAACGAGGATAAGCAATGTGGCGGACTTGGATGGGTCCTTCGACGTTGGGACGAAGAACTCATGGTCGCTGGGTACCGGTACATCAAGCGTCCATGGCGAGTCAGCTGGCTTGAAACGATTGCAATAATGGAAGGCCTTCAATCGATTTCGTCATCTTCCCCACCGGTGCGTGTTGAAACGGATTCTCTACAAGTGGCGCGGCTACTCAATGACAAAGACAGGGATGAAACAAAGTTGAGGGTGTTTGTTTTGGAAGCAAAAGCCCTAACCTCTTCTAAGAATATTGAATCTATAGCTCATGTGACTGGAATTCATAACGTTGTGGCCCACAACTTGGCTATAAAGGCTAGTGAAAGTGAAGCATCACATAGTTGGTTTGGAAATTTTCCATCTTGGGTTCACCCTCCTAATGAGGTGGATATTGGACATGTAAATCATGCCTATGGGGTTCCTGTCCCATAG

mRNA sequence

ATGGGCGCCGGGACTCAACTCACCCGCCTTATCGTCATCCCTGACCCTGTGGCGGATTCTGGATTTAATTTGATGGAGTGCAAGATTGGATTTGGGTTGGAATCGTATTGCGTCAACCCGACAGTGACCATTCCTGCAGCGGCGGCTTCAAGGACGAACTTGAACCCCCAGCTTCTATGGTTGTCGATCCCTACCAGCATATGGAAGCTTAGCTGCGACGCGTCCTGGAACGAGGATAAGCAATGTGGCGGACTTGGATGGGTCCTTCGACGTTGGGACGAAGAACTCATGGTCGCTGGGTACCGGTACATCAAGCGTCCATGGCGAGTCAGCTGGCTTGAAACGATTGCAATAATGGAAGGCCTTCAATCGATTTCGTCATCTTCCCCACCGGTGCGTGTTGAAACGGATTCTCTACAAGTGGCGCGGCTACTCAATGACAAAGACAGGGATGAAACAAAGTTGAGGGTGTTTGTTTTGGAAGCAAAAGCCCTAACCTCTTCTAAGAATATTGAATCTATAGCTCATGTGACTGGAATTCATAACGTTGTGGCCCACAACTTGGCTATAAAGGCTAGTGAAAGTGAAGCATCACATAGTTGGTTTGGAAATTTTCCATCTTGGGTTCACCCTCCTAATGAGGTGGATATTGGACATGTAAATCATGCCTATGGGGTTCCTGTCCCATAG

Coding sequence (CDS)

ATGGGCGCCGGGACTCAACTCACCCGCCTTATCGTCATCCCTGACCCTGTGGCGGATTCTGGATTTAATTTGATGGAGTGCAAGATTGGATTTGGGTTGGAATCGTATTGCGTCAACCCGACAGTGACCATTCCTGCAGCGGCGGCTTCAAGGACGAACTTGAACCCCCAGCTTCTATGGTTGTCGATCCCTACCAGCATATGGAAGCTTAGCTGCGACGCGTCCTGGAACGAGGATAAGCAATGTGGCGGACTTGGATGGGTCCTTCGACGTTGGGACGAAGAACTCATGGTCGCTGGGTACCGGTACATCAAGCGTCCATGGCGAGTCAGCTGGCTTGAAACGATTGCAATAATGGAAGGCCTTCAATCGATTTCGTCATCTTCCCCACCGGTGCGTGTTGAAACGGATTCTCTACAAGTGGCGCGGCTACTCAATGACAAAGACAGGGATGAAACAAAGTTGAGGGTGTTTGTTTTGGAAGCAAAAGCCCTAACCTCTTCTAAGAATATTGAATCTATAGCTCATGTGACTGGAATTCATAACGTTGTGGCCCACAACTTGGCTATAAAGGCTAGTGAAAGTGAAGCATCACATAGTTGGTTTGGAAATTTTCCATCTTGGGTTCACCCTCCTAATGAGGTGGATATTGGACATGTAAATCATGCCTATGGGGTTCCTGTCCCATAG

Protein sequence

MGAGTQLTRLIVIPDPVADSGFNLMECKIGFGLESYCVNPTVTIPAAAASRTNLNPQLLWLSIPTSIWKLSCDASWNEDKQCGGLGWVLRRWDEELMVAGYRYIKRPWRVSWLETIAIMEGLQSISSSSPPVRVETDSLQVARLLNDKDRDETKLRVFVLEAKALTSSKNIESIAHVTGIHNVVAHNLAIKASESEASHSWFGNFPSWVHPPNEVDIGHVNHAYGVPVP

Homology

[Close]

BLAST of Spg001648 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 110.9 bits (276), Expect = 1.4e-20
Identity = 62/158 (39.24%), Postives = 90/158 (56.96%), Query Frame = 0


Query: 54  LNPQLLWLSIPTSIWKLSCDASWNEDKQCGGLGWVLRRWDEELMVAGYRYIKRPWRVSWL 113
           LN +L W   P  IW L+ DASW++    GG+GW++R WD ++++AG R+++    V  L
Sbjct: 65  LNNKLKWEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLL 124

Query: 114 ETIAIMEGLQSISSSS--PPVRVETDSLQVARLLNDKDRDETKLRVFVLEAKALTSSKNI 173
           E  AI+EGL+++++     P+ +ETDS +V  LLN K  D TK    V E   L  S  I
Sbjct: 125 EASAILEGLRNLTNLGVLRPLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEI 184

Query: 174 ESIAHVTGIHNVVAHNLAIKASESEASHSWFGNFPSWV 210
            + A V    N  AH+LA +AS    S  W   FP+W+
Sbjct: 185 LAFAKVGRETNGCAHSLAQRASVLRESMIWVDCFPNWL 222

[Close]

BLAST of Spg001648 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 87.0 bits (214), Expect = 2.2e-13
Identity = 46/138 (33.33%), Postives = 80/138 (57.97%), Query Frame = 0


Query: 60  WLSIPTSIWKLSCDASWNEDKQCGGLGWVLRRWDEELMVAGYRYIKRPWRVSWLETIAIM 119
           W    ++ WKL+ +A+W  D   GG+GW+LR    E++ A  R I+    +++LE +AI 
Sbjct: 79  WKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAIC 138

Query: 120 EGLQSI-SSSSPPVRVETDSLQVARLLNDKDRDETKLRVFVLEAKALTSSKNIESIAHVT 179
           EGL++I      P+ +E+DSL+   LL+ + +D+T++   + E   +     I S+ H++
Sbjct: 139 EGLRAIRQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHIS 198

Query: 180 GIHNVVAHNLAIKASESE 197
              N VAH LA +A E++
Sbjct: 199 REANKVAHGLARRAMEND 216

[Close]

BLAST of Spg001648 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 85.9 bits (211), Expect = 5.0e-13
Identity = 47/146 (32.19%), Postives = 81/146 (55.48%), Query Frame = 0


Query: 60  WLSIPTSIWKLSCDASWNEDKQCGGLGWVLRRWDEELMVAGYRYIKRPWRVSWLETIAIM 119
           W    ++ WKL+ DA+W  D   GG+GW+LR    E++ A  R I+    +++LE +AI 
Sbjct: 79  WKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAIC 138

Query: 120 EGLQSI---------SSSSPPVRVETDSLQVARLLNDKDRDETKLRVFVLEAKALTSSKN 179
           EGL++I              P+ +E+DSL+   LL+ + +D+T++   + E   +     
Sbjct: 139 EGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMK 198

Query: 180 IESIAHVTGIHNVVAHNLAIKASESE 197
           I S+ H++   N VAH+LA +A E++
Sbjct: 199 IVSMRHISREANKVAHDLARRAMEND 224

[Close]

BLAST of Spg001648 vs. NCBI nr
Match: XP_022143317.1 (uncharacterized protein LOC111013216 [Momordica charantia])

HSP 1 Score: 81.3 bits (199), Expect = 1.2e-11
Identity = 42/105 (40.00%), Postives = 68/105 (64.76%), Query Frame = 0


Query: 51  RTNLNP-QLLWLSIPTSIWKLSCDASWNEDKQCGGLGWVLRRWDEELMVAGYRYIKRPWR 110
           R NLN   + W + PT+ WKL+ DASW+E+++ GG+GW+L     E+++AG   I+    
Sbjct: 95  RENLNMLGVRWSAPPTNCWKLNTDASWSEEREVGGIGWILCDCRGEIVLAGNCKIREKKE 154

Query: 111 VSWLETIAIMEGLQSIS-SSSPPVRVETDSLQVARLLNDKDRDET 154
           ++ LE + I+ GLQ I+  S  P+ +E+DS++V RL+  +D D T
Sbjct: 155 INALELMTIIRGLQFINMQSRSPIYLESDSVEVIRLMKKEDVDLT 199

[Close]

BLAST of Spg001648 vs. NCBI nr
Match: XP_022148737.1 (uncharacterized protein LOC111017329 [Momordica charantia])

HSP 1 Score: 73.9 bits (180), Expect = 2.0e-09
Identity = 40/132 (30.30%), Postives = 75/132 (56.82%), Query Frame = 0


Query: 64  PTSIWKLSCDASWNEDKQCGGLGWVLRRWDEELMVAGYRYIKRPWRVSWLETIAIMEGLQ 123
           P + WKL+ DA+W+ ++  GGLGW++R    E+  AG + I     +++LE +AI  G++
Sbjct: 9   PDNFWKLNTDAAWHANQGVGGLGWIIRNEKREIKKAGGQTITDARDITYLELMAIWLGIE 68

Query: 124 S-ISSSSPPVRVETDSLQVARLLNDKDRDETKLRVFVLEAKALTSSKNIESIAHVTGIHN 183
           + +SSSS  + +E++SL+   L+    ++ T++   V + K       ++   HV    N
Sbjct: 69  AVVSSSSVSLIIESESLEAIHLIQGIQQNVTEILWLVQDIKNFGERARLKRFQHVQRDGN 128

Query: 184 VVAHNLAIKASE 195
            VA  +A +A++
Sbjct: 129 SVAQEIANRAAD 140

[Close]

BLAST of Spg001648 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 7.0e-21
Identity = 62/158 (39.24%), Postives = 90/158 (56.96%), Query Frame = 0


Query: 54  LNPQLLWLSIPTSIWKLSCDASWNEDKQCGGLGWVLRRWDEELMVAGYRYIKRPWRVSWL 113
           LN +L W   P  IW L+ DASW++    GG+GW++R WD ++++AG R+++    V  L
Sbjct: 65  LNNKLKWEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLL 124

Query: 114 ETIAIMEGLQSISSSS--PPVRVETDSLQVARLLNDKDRDETKLRVFVLEAKALTSSKNI 173
           E  AI+EGL+++++     P+ +ETDS +V  LLN K  D TK    V E   L  S  I
Sbjct: 125 EASAILEGLRNLTNLGVLRPLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEI 184

Query: 174 ESIAHVTGIHNVVAHNLAIKASESEASHSWFGNFPSWV 210
            + A V    N  AH+LA +AS    S  W   FP+W+
Sbjct: 185 LAFAKVGRETNGCAHSLAQRASVLRESMIWVDCFPNWL 222

[Close]

BLAST of Spg001648 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 1.1e-13
Identity = 46/138 (33.33%), Postives = 80/138 (57.97%), Query Frame = 0


Query: 60  WLSIPTSIWKLSCDASWNEDKQCGGLGWVLRRWDEELMVAGYRYIKRPWRVSWLETIAIM 119
           W    ++ WKL+ +A+W  D   GG+GW+LR    E++ A  R I+    +++LE +AI 
Sbjct: 79  WKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAIC 138

Query: 120 EGLQSI-SSSSPPVRVETDSLQVARLLNDKDRDETKLRVFVLEAKALTSSKNIESIAHVT 179
           EGL++I      P+ +E+DSL+   LL+ + +D+T++   + E   +     I S+ H++
Sbjct: 139 EGLRAIRQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHIS 198

Query: 180 GIHNVVAHNLAIKASESE 197
              N VAH LA +A E++
Sbjct: 199 REANKVAHGLARRAMEND 216

[Close]

BLAST of Spg001648 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 2.4e-13
Identity = 47/146 (32.19%), Postives = 81/146 (55.48%), Query Frame = 0


Query: 60  WLSIPTSIWKLSCDASWNEDKQCGGLGWVLRRWDEELMVAGYRYIKRPWRVSWLETIAIM 119
           W    ++ WKL+ DA+W  D   GG+GW+LR    E++ A  R I+    +++LE +AI 
Sbjct: 79  WKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAIC 138

Query: 120 EGLQSI---------SSSSPPVRVETDSLQVARLLNDKDRDETKLRVFVLEAKALTSSKN 179
           EGL++I              P+ +E+DSL+   LL+ + +D+T++   + E   +     
Sbjct: 139 EGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMK 198

Query: 180 IESIAHVTGIHNVVAHNLAIKASESE 197
           I S+ H++   N VAH+LA +A E++
Sbjct: 199 IVSMRHISREANKVAHDLARRAMEND 224

[Close]

BLAST of Spg001648 vs. ExPASy TrEMBL
Match: A0A6J1CQG0 (uncharacterized protein LOC111013216 OS=Momordica charantia OX=3673 GN=LOC111013216 PE=4 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 5.9e-12
Identity = 42/105 (40.00%), Postives = 68/105 (64.76%), Query Frame = 0


Query: 51  RTNLNP-QLLWLSIPTSIWKLSCDASWNEDKQCGGLGWVLRRWDEELMVAGYRYIKRPWR 110
           R NLN   + W + PT+ WKL+ DASW+E+++ GG+GW+L     E+++AG   I+    
Sbjct: 95  RENLNMLGVRWSAPPTNCWKLNTDASWSEEREVGGIGWILCDCRGEIVLAGNCKIREKKE 154

Query: 111 VSWLETIAIMEGLQSIS-SSSPPVRVETDSLQVARLLNDKDRDET 154
           ++ LE + I+ GLQ I+  S  P+ +E+DS++V RL+  +D D T
Sbjct: 155 INALELMTIIRGLQFINMQSRSPIYLESDSVEVIRLMKKEDVDLT 199

[Close]

BLAST of Spg001648 vs. ExPASy TrEMBL
Match: A0A6J1D5W1 (uncharacterized protein LOC111017329 OS=Momordica charantia OX=3673 GN=LOC111017329 PE=4 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 9.5e-10
Identity = 40/132 (30.30%), Postives = 75/132 (56.82%), Query Frame = 0


Query: 64  PTSIWKLSCDASWNEDKQCGGLGWVLRRWDEELMVAGYRYIKRPWRVSWLETIAIMEGLQ 123
           P + WKL+ DA+W+ ++  GGLGW++R    E+  AG + I     +++LE +AI  G++
Sbjct: 9   PDNFWKLNTDAAWHANQGVGGLGWIIRNEKREIKKAGGQTITDARDITYLELMAIWLGIE 68

Query: 124 S-ISSSSPPVRVETDSLQVARLLNDKDRDETKLRVFVLEAKALTSSKNIESIAHVTGIHN 183
           + +SSSS  + +E++SL+   L+    ++ T++   V + K       ++   HV    N
Sbjct: 69  AVVSSSSVSLIIESESLEAIHLIQGIQQNVTEILWLVQDIKNFGERARLKRFQHVQRDGN 128

Query: 184 VVAHNLAIKASE 195
            VA  +A +A++
Sbjct: 129 SVAQEIANRAAD 140

The following BLAST results are available for this feature:

BLAST of Spg001648 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022155262.1	1.4e-20	39.24	uncharacterized protein LOC111022403 [Momordica charantia]	[more]
XP_022143535.1	2.2e-13	33.33	uncharacterized protein LOC111013412 [Momordica charantia]	[more]
XP_022156777.1	5.0e-13	32.19	uncharacterized protein LOC111023608 [Momordica charantia]	[more]
XP_022143317.1	1.2e-11	40.00	uncharacterized protein LOC111013216 [Momordica charantia]	[more]
XP_022148737.1	2.0e-09	30.30	uncharacterized protein LOC111017329 [Momordica charantia]	[more]

BLAST of Spg001648 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Spg001648 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DNV9	7.0e-21	39.24	uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A6J1CP26	1.1e-13	33.33	uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A6J1DSV1	2.4e-13	32.19	uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6J1CQG0	5.9e-12	40.00	uncharacterized protein LOC111013216 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A6J1D5W1	9.5e-10	30.30	uncharacterized protein LOC111017329 OS=Momordica charantia OX=3673 GN=LOC111017...	[more]

BLAST of Spg001648 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 67..194 e-value: 1.2E-9 score: 40.3
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 72..191 e-value: 3.5E-13 score: 49.5
None	No IPR available	PANTHER	PTHR33033:SF67	SUBFAMILY NOT NAMED	coord: 57..210
None	No IPR available	PANTHER	PTHR33033	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 57..210
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 70..189 e-value: 2.40476E-12 score: 60.0204
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 67..194

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg001648.1	Spg001648.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity