Spg001424 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg001424 vs. NCBI nr
Match: XP_022146229.1 (putative amidase C869.01 [Momordica charantia])

HSP 1 Score: 743.0 bits (1917), Expect = 1.6e-210
Identity = 401/510 (78.63%), Postives = 426/510 (83.53%), Query Frame = 0

Query: 1   METQSFSLSFSLFLILVAVSPFGSYSA-GTGFSIKEATLKDLQLAFKQNKLTSRQLVEFY 60
           MET   SLSFSLFLILVAVSPF SYS    GF I+EA LKDL LAFKQNKLTSRQLVEFY
Sbjct: 1   METPRLSLSFSLFLILVAVSPFWSYSVRPAGFPIEEAGLKDLHLAFKQNKLTSRQLVEFY 60

Query: 61  IKQIRRYNPLLKGVIEVNPDALYLADNADRERKAKAPGSRSLLHGIPVLVKDNIATKDKL 120
           IKQIRRYNP L+GVIEVNPDALYLAD ADRERKAKAPGS  LLHGIPVLVKDN+ATKDKL
Sbjct: 61  IKQIRRYNPRLRGVIEVNPDALYLADKADRERKAKAPGSLPLLHGIPVLVKDNMATKDKL 120

Query: 121 NTTAGSFALLGSVVPRDAGVVTRLRKAGAIILGKASLSEWAYFRSYGAPSGWSARAGHGR 180
           NTTAGS ALLGSVVPRDAG V RLRKAGAIILGKAS+SEWA FRS GAP+GW+ARAG GR
Sbjct: 121 NTTAGSLALLGSVVPRDAGAVMRLRKAGAIILGKASMSEWAGFRSNGAPTGWNARAGQGR 180

Query: 181 EPYTLGDPCGSSSGSAISVSANMAAVTLGTETDGSILCPSSQNSVVGIKPTIGLTSRAGV 240
           EPYTLG PCGSSSGSAISVSANMAAV LGTETDGSILCPSS NSVVGIKPT+GLTS AGV
Sbjct: 181 EPYTLGLPCGSSSGSAISVSANMAAVALGTETDGSILCPSSFNSVVGIKPTLGLTSTAGV 240

Query: 241 VPISPRQDTVGPICRTVSDAAYVLDVIVGTDALDDETYKASRYIPRGGYGQFLRANGLKG 300
           VPISPRQDTVGPICRTVSDAAYVLDVIVGTD LD+ TYKASRYIPRGGYGQFL+A+GL+G
Sbjct: 241 VPISPRQDTVGPICRTVSDAAYVLDVIVGTDPLDNSTYKASRYIPRGGYGQFLKADGLRG 300

Query: 301 KRLGIVEDLFSVTEPFLIQAFEEIFTILRKRGAILVNNLKIDNLGIIF-SAESGEDIALL 360
           KRLGIVED F V +P LI AFEEIFTIL KRGAILV+NLKIDNLG I+ + ESGE  ALL
Sbjct: 301 KRLGIVEDFFGVVDPSLIPAFEEIFTILSKRGAILVDNLKIDNLGAIYNNTESGEVAALL 360

Query: 361 NEFK-------------------------------EKIKEYRQDLFLQAEKTKGIGMEAV 420
           NEFK                               EKIKEY QDLFLQAE T G+G+  +
Sbjct: 361 NEFKVSLNAYLKELVSSPIRSLAEAIAFNQKHSSLEKIKEYGQDLFLQAEATNGMGINGL 420

Query: 421 LSKLAKLSKDGLEKTITENKLDAIVTPDSSISTLLAIGGFPGITVPAGYLPKGTPFGIHF 478
           LSKL KLSKDGLEKT+ +NKLDAI+TP + IS +LAIGGFPGITVPAGY P GTPFGI F
Sbjct: 421 LSKLGKLSKDGLEKTMIKNKLDAIMTPSAIISPILAIGGFPGITVPAGYSPSGTPFGIDF 480

BLAST of Spg001424 vs. NCBI nr
Match: XP_004147023.1 (probable amidase At4g34880 [Cucumis sativus] >KGN61907.1 hypothetical protein Csa_006678 [Cucumis sativus])

HSP 1 Score: 614.4 bits (1583), Expect = 8.4e-172
Identity = 334/512 (65.23%), Postives = 386/512 (75.39%), Query Frame = 0

Query: 1   METQSFSLSFSLFLILVAV-SPFGSYSAGTGFSIKEATLKDLQLAFKQNKLTSRQLVEFY 60
           M  QSF L  S+ L L+A+ S  GS S  T  S++EATLKDLQ AF QNKLTSRQLVEFY
Sbjct: 1   MAGQSFLLDISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRQLVEFY 60

Query: 61  IKQIRRYNPLLKGVIEVNPDALYLADNADRERKAKAPGSRSLLHGIPVLVKDNIATKDKL 120
           ++Q+RR NP+LKG+IEVNPDAL  A  AD ERK  +P S S LHGIPVLVKDNIATKDKL
Sbjct: 61  LEQVRRLNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 121 NTTAGSFALLGSVVPRDAGVVTRLRKAGAIILGKASLSEWAYFRSYGAPSGWSARAGHGR 180
           NTTAGSFALLGSVVPRDAGVVT+LR AGAII GKASLSEW+YFRS   PSGWSAR G G+
Sbjct: 121 NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNALPSGWSARGGQGK 180

Query: 181 EPYTLGDPCGSSSGSAISVSANMAAVTLGTETDGSILCPSSQNSVVGIKPTIGLTSRAGV 240
            PYT+G+PCGSSSGSAISV+ANM  V+LGTETDGSILCPS+ NSVVGIKPT+GLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 VPISPRQDTVGPICRTVSDAAYVLDVIVGTDALDDETYKASRYIPRGGYGQFLRANGLKG 300
           VPIS RQDTVGPICRTVSDAAYVL+ IVG D  D+ T +AS+YIP+GGYGQFLRA GLKG
Sbjct: 241 VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 301 KRLGIVEDL--FSVTEPFLIQAFEEIFTILRKRGAILVNNLKIDNLGIIFSAESGEDIAL 360
           KR+GIV +   F   + F  QA+E++   L+K GAILV+NL IDNL  IF   SGE IAL
Sbjct: 301 KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILVDNLMIDNLEQIFDGSSGEQIAL 360

Query: 361 LNEFKEKIK------------------------------EYRQDLFLQAEKTKGIG--ME 420
           L EFK  +                               EY Q+ FL+AE T GIG   +
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQEEFLKAEATNGIGDAEK 420

Query: 421 AVLSKLAKLSKDGLEKTITENKLDAIVTPDSSISTLLAIGGFPGITVPAGYLPKGTPFGI 478
           A L++LAKLSKDG E+ + +NKLDA+  P S IS + AIGGFPG++VPAGY P+G P+GI
Sbjct: 421 AALARLAKLSKDGFERLMIKNKLDAVAAPGSLISPVFAIGGFPGVSVPAGYDPQGNPYGI 480

BLAST of Spg001424 vs. NCBI nr
Match: XP_008457661.1 (PREDICTED: putative amidase C869.01 [Cucumis melo] >ADN34008.1 amidase [Cucumis melo subsp. melo] >KAA0045711.1 putative amidase [Cucumis melo var. makuwa] >TYJ99569.1 putative amidase [Cucumis melo var. makuwa])

HSP 1 Score: 610.9 bits (1574), Expect = 9.3e-171
Identity = 332/511 (64.97%), Postives = 385/511 (75.34%), Query Frame = 0

Query: 1   METQSFSLSFSLFLILVAV-SPFGSYSAGTGFSIKEATLKDLQLAFKQNKLTSRQLVEFY 60
           M  QSF L  S+ L L+A+ S +GS S  T  SI+EATL DLQ AF QNKLTSRQLVEFY
Sbjct: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60

Query: 61  IKQIRRYNPLLKGVIEVNPDALYLADNADRERKAKAPGSRSLLHGIPVLVKDNIATKDKL 120
           ++Q+RR+NP+LKG+IEVNPDAL  A  AD ERK  +P S S LHGIPVLVKDNIATKDKL
Sbjct: 61  LEQVRRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 121 NTTAGSFALLGSVVPRDAGVVTRLRKAGAIILGKASLSEWAYFRSYGAPSGWSARAGHGR 180
           NTTAGSFALLGS+VPRDAGVVT+LR AGAII GKASLSEW+YFRS   PSGWSAR G G+
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180

Query: 181 EPYTLGDPCGSSSGSAISVSANMAAVTLGTETDGSILCPSSQNSVVGIKPTIGLTSRAGV 240
            PY LG+PCGSSSGSAISV+ANM  V+LGTETDGSILCPS+ NSVVGIKPT+GLTSRAGV
Sbjct: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 VPISPRQDTVGPICRTVSDAAYVLDVIVGTDALDDETYKASRYIPRGGYGQFLRANGLKG 300
           +PIS RQD+VGPICRTVSDA YVLD IVG D  D+ T +AS+YIP+GGYGQFLRA GLKG
Sbjct: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 301 KRLGIVEDL--FSVTEPFLIQAFEEIFTILRKRGAILVNNLKIDNLGIIFSAESGEDIAL 360
           KR+GIV +   F   E F  QA+E++   L+K GAILV+N  IDNL +IF   SGE IAL
Sbjct: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360

Query: 361 LNEFKEKIK------------------------------EYRQDLFLQAEKTKGIG--ME 420
           L EFK  +                               EY Q+LFL+AE T GIG   +
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQELFLKAEATNGIGDAEK 420

Query: 421 AVLSKLAKLSKDGLEKTITENKLDAIVTPDSSISTLLAIGGFPGITVPAGYLPKGTPFGI 477
           A L++LAKLSKDG E+ + +NKLDAI  P S IS++ AIGGFPG++VPAGY P+G P+GI
Sbjct: 421 AALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGVSVPAGYDPQGVPYGI 480

BLAST of Spg001424 vs. NCBI nr
Match: XP_038900912.1 (LOW QUALITY PROTEIN: probable amidase At4g34880 [Benincasa hispida])

HSP 1 Score: 610.5 bits (1573), Expect = 1.2e-170
Identity = 327/513 (63.74%), Postives = 388/513 (75.63%), Query Frame = 0

Query: 1   METQSFSLSFSLFLILVAV-SPFGSYSAGTGFSIKEATLKDLQLAFKQNKLTSRQLVEFY 60
           M  QSF L  S+ L L+A+ S +GS S  T  SI+EAT+KDLQLAF QNKL SRQLV+FY
Sbjct: 6   MAAQSFWLYISMLLSLLAILSSYGSCSFDTHLSIEEATVKDLQLAFYQNKLISRQLVKFY 65

Query: 61  IKQIRRYNPLLKGVIEVNPDALYLADNADRERKAKAPGSRSLLHGIPVLVKDNIATKDKL 120
           +  +RR+NP+LKG+IEVNPDAL  A  AD ERK  +P S S LHGIPVLVKDNIAT+DKL
Sbjct: 66  LNXVRRFNPILKGIIEVNPDALDQASQADLERKRNSPSSLSPLHGIPVLVKDNIATRDKL 125

Query: 121 NTTAGSFALLGSVVPRDAGVVTRLRKAGAIILGKASLSEWAYFRSYGAPSGWSARAGHGR 180
           NTTAGSFALLGS+VPRDAGVVT+LRKAGAII GKASLS+W+YFRSY  PSGWSAR G G+
Sbjct: 126 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSKWSYFRSYXIPSGWSARGGQGK 185

Query: 181 EPYTLGDPCGSSSGSAISVSANMAAVTLGTETDGSILCPSSQNSVVGIKPTIGLTSRAGV 240
            PYT+G+PCGSS GSAIS++ANM AV+LGTETDGSILCPS  NSVVGIKPT+GLTSRAGV
Sbjct: 186 NPYTMGEPCGSSGGSAISIAANMVAVSLGTETDGSILCPSILNSVVGIKPTVGLTSRAGV 245

Query: 241 VPISPRQDTVGPICRTVSDAAYVLDVIVGTDALDDETYKASRYIPRGGYGQFLRANGLKG 300
           +PIS RQDTVGPICRTVSD AYVL  IVG D  D+ T +AS+YIPRGGY QFLRA+GLKG
Sbjct: 246 IPISLRQDTVGPICRTVSDDAYVLGAIVGADKNDNSTIEASKYIPRGGYAQFLRADGLKG 305

Query: 301 KRLGIVEDLFSV--TEPFLIQAFEEIFTILRKRGAILVNNLKIDNLGIIFSAESGEDIAL 360
           KR+GIV   F     + F  +A+E++F  LR+ GA+ V+NL ID+L +I  + SGE+ AL
Sbjct: 306 KRIGIVRKFFDFGHDDAFYPRAYEKVFKTLRQGGAVFVDNLTIDSLQVIMGSSSGEETAL 365

Query: 361 LNEFK-------------------------------EKIKEYRQDLFLQAEKTKGIG--M 420
           L EFK                               EK++EY Q+LF++AE TKGIG   
Sbjct: 366 LAEFKISLNAYLKQLVASPIRSLSDAIEFNEKNSKLEKLREYGQNLFIEAEATKGIGDAE 425

Query: 421 EAVLSKLAKLSKDGLEKTITENKLDAIVTPDSSISTLLAIGGFPGITVPAGYLPKGTPFG 478
           +A L++LAKLSKDG E+ + +NKLDAI  P   IS++LAIGGFPGI+VPAGY  +G PFG
Sbjct: 426 KAALARLAKLSKDGFERVMLKNKLDAIAAPGWEISSVLAIGGFPGISVPAGYNSQGVPFG 485

BLAST of Spg001424 vs. NCBI nr
Match: XP_004147024.3 (probable amidase At4g34880 [Cucumis sativus])

HSP 1 Score: 607.1 bits (1564), Expect = 1.3e-169
Identity = 328/512 (64.06%), Postives = 388/512 (75.78%), Query Frame = 0

Query: 1   METQSFSLSFSLFLILVAV-SPFGSYSAGTGFSIKEATLKDLQLAFKQNKLTSRQLVEFY 60
           M  QSF +  S+ L L+A+ S +GS S  T FSI+EATLKDLQLAF QNKLTS QLVEFY
Sbjct: 1   MAAQSFPIYISMLLGLLAILSSYGSCSFDTNFSIEEATLKDLQLAFYQNKLTSTQLVEFY 60

Query: 61  IKQIRRYNPLLKGVIEVNPDALYLADNADRERKAKAPGSRSLLHGIPVLVKDNIATKDKL 120
           ++Q+RR+NP+L G+IEVNPDAL  A  AD ERK  +P S S LHGIPVLVKDNIATKD+L
Sbjct: 61  LEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVLVKDNIATKDQL 120

Query: 121 NTTAGSFALLGSVVPRDAGVVTRLRKAGAIILGKASLSEWAYFRSYGAPSGWSARAGHGR 180
           NTTAGSFALLGS+VPRDAGVVT+LRKAGAII GKASLSEW+ FRSY  P+GWSAR G G+
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEQPNGWSARGGQGK 180

Query: 181 EPYTLGDPCGSSSGSAISVSANMAAVTLGTETDGSILCPSSQNSVVGIKPTIGLTSRAGV 240
            PYT+G+PCGSSSGSAISV+ANM  V+LGTETDGSILCPS+ NSVVGIKPT+GLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 VPISPRQDTVGPICRTVSDAAYVLDVIVGTDALDDETYKASRYIPRGGYGQFLRANGLKG 300
           VPIS RQDTVGPICRTV+DAAYVLD I G D  D+ T +AS+Y+P+GGYGQFL+ +GLKG
Sbjct: 241 VPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYVPKGGYGQFLKEDGLKG 300

Query: 301 KRLGIVEDL--FSVTEPFLIQAFEEIFTILRKRGAILVNNLKIDNLGIIFSAESGEDIAL 360
           KR+GIV  L  F   + F I AFE++F  L++ GAILV+NL I++  +I  + SGE  A+
Sbjct: 301 KRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINSFHVITGSSSGEWTAV 360

Query: 361 LNEFK-------------------------------EKIKEYRQDLFLQAEKTKGIG--M 420
           L EFK                               EK+KEY Q+LFL+AE TKGIG   
Sbjct: 361 LAEFKISINVYLKQLVASPIRSLSDAIEFNRKNSKLEKLKEYGQELFLEAEATKGIGGAE 420

Query: 421 EAVLSKLAKLSKDGLEKTITENKLDAIVTPDSSISTLLAIGGFPGITVPAGYLPKGTPFG 477
           +A L++LAKLSK+G E+ + +NKLDAI  P   IS  LAIGGFPG++VPAGY P+G PFG
Sbjct: 421 KAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISHFLAIGGFPGVSVPAGYNPQGLPFG 480

BLAST of Spg001424 vs. ExPASy Swiss-Prot
Match: A0A1P8B760 (Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 1.9e-142
Identity = 287/508 (56.50%), Postives = 358/508 (70.47%), Query Frame = 0

Query: 10  FSLFLILVAVSPFGSYSAGTG--------FSIKEATLKDLQLAFKQNKLTSRQLVEFYIK 69
           FS  LIL+ +S     S G+         FSI+EAT++D+++AF + +LTS+QLVE Y++
Sbjct: 9   FSSLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLE 68

Query: 70  QIRRYNPLLKGVIEVNPDALYLADNADRERKAKAPGSRSLLHGIPVLVKDNIATKDKLNT 129
            I + NP+L  VIE NPDAL  A+ ADRER  K      +LHG+PVL+KD+I+TKDKLNT
Sbjct: 69  AISKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDKLNT 128

Query: 130 TAGSFALLGSVVPRDAGVVTRLRKAGAIILGKASLSEWAYFRSYGAPSGWSARAGHGREP 189
           TAGSFALLGSVV RDAGVV RLR++GA+ILGKASLSEWA+FRS+  P GWSAR   G+ P
Sbjct: 129 TAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSARGLQGKNP 188

Query: 190 YTL-GDPCGSSSGSAISVSANMAAVTLGTETDGSILCPSSQNSVVGIKPTIGLTSRAGVV 249
           Y L  +P GSSSGSAISV+AN+ AV+LGTETDGSIL P+SQNSVVGIKP++GLTSRAGVV
Sbjct: 189 YVLSANPSGSSSGSAISVTANLVAVSLGTETDGSILSPASQNSVVGIKPSVGLTSRAGVV 248

Query: 250 PISPRQDTVGPICRTVSDAAYVLDVIVGTDALDDETYKASRYIPRGGYGQFLRANGLKGK 309
           PIS RQD++GPICRTVSDA ++LD IVG D LD+ T  AS +IP GGY QFL  +GLKGK
Sbjct: 249 PISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLTTSGLKGK 308

Query: 310 RLGIVEDLFSVTEPFLIQAFEEIFTILRKRGAILVNNLKIDNLGIIF-SAESGEDIALLN 369
           RLGIV    S+ +  +          LR+ GAI++NNL I N+ +I    +SGE+IALL 
Sbjct: 309 RLGIVMKHSSLLDHHI--------KTLRREGAIVINNLTIPNIEVIVGGTDSGEEIALLA 368

Query: 370 EFK-------------------------------EKIKEYRQDLFLQAEKTKGIG--MEA 429
           EFK                               EK+KE+ Q++FL AE T G+G   + 
Sbjct: 369 EFKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKEKT 428

Query: 430 VLSKLAKLSKDGLEKTITENKLDAIVTPDSSISTLLAIGGFPGITVPAGYLPKGTPFGIH 475
            L K+ +LS++G+EK I ENKLDAIVT  S +S++LAIGG+PGI VPAGY   G P+GI 
Sbjct: 429 ALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYGIS 488

BLAST of Spg001424 vs. ExPASy Swiss-Prot
Match: D4B3C8 (Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02965 PE=1 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 1.4e-68
Identity = 181/491 (36.86%), Postives = 259/491 (52.75%), Query Frame = 0

Query: 41  LQLAFKQNKLTSRQLVEFYIKQIRRYNPLLKGVIEVNPDALYLADNADRERKAKAPGSRS 100
           LQ  ++Q  +    +V+ Y+ +I   N  ++ V E+NPDAL +A   D ERK      R 
Sbjct: 94  LQTPYRQGNVLIIVVVQTYVARIAEVNSTVRAVTEINPDALTIAKQMDNERKMGK--LRG 153

Query: 101 LLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTRLRKAGAIILGKASLSEWA 160
            LHG+P+++K+NI T DK+++TAGS+A+ G+    DA V T+LR+AG +I+GK+  S+WA
Sbjct: 154 PLHGLPIVIKNNIFTDDKMSSTAGSYAIFGARTSADATVATKLREAGLVIMGKSGASQWA 213

Query: 161 YFRSYGAPSGWSARAGHGREPYTLG-DPCGSSSGSAISVSANMAAVTLGTETDGSILCPS 220
            FRS  + +GWSA  G     Y    DP GSSSGS ++    +A  TLGTET GSI+ P+
Sbjct: 214 NFRSLNSTNGWSAYGGQVTAAYIKNQDPSGSSSGSGVASDLGLAFATLGTETSGSIVSPA 273

Query: 221 SQNSVVGIKPTIGLTSRAGVVPISPRQDTVGPICRTVSDAAYVLDVIVGTDALDDETYKA 280
            ++++VG+KPT+GLTSR  VVPIS RQDTVGP+ R+V DAAY+L VI G D+ D+ T  A
Sbjct: 274 DKSNIVGLKPTVGLTSRRFVVPISERQDTVGPMARSVKDAAYLLQVIAGKDSNDNYT-SA 333

Query: 281 SRYIPRGGYGQFLRANGLKGKRLGIVEDLFSV--TEPFLIQAFEEIFTILRKRGAILVNN 340
             +     Y +    N LKGKR+G+  ++  +  +   ++  F +   +++K GAI+V N
Sbjct: 334 IPFDTIPDYVKACDINALKGKRIGVPRNVIKIFGSPQTVVDQFNQALAVMKKAGAIIVEN 393

Query: 341 L---------------------KIDNLGIIF-----SAESGEDIALLNEFKE--KIKEY- 400
                                  + NL   F     +  +  D+  L  F +  +++EY 
Sbjct: 394 TDFTSFAEFAQSPIPDDILYADSLTNLPAFFKQLKVNPHNITDLESLRRFTQHHRLEEYP 453

Query: 401 -----RQDLFLQ-AEKTKGIGMEAVLSKLAKLSKD-GLEKTITENKLDAIVTPDSSISTL 460
                R D+ LQ   K        +  K  K   + G+   +  +KLDA V P      +
Sbjct: 454 SRDTARWDIALQKGIKNTDPKFWPMYQKNVKFGNEGGILGALRRHKLDAAVLPTDLSPYI 513

Query: 461 LAIGGFPGITVPAGYLPKGT---------------PFGIHFGGLKGFEPKLIEIAYDFEQ 478
            A+ G P ITVP G  P GT               P GI F G    E KLI +AY FEQ
Sbjct: 514 PALIGSPIITVPMGVYPNGTKVNHDRELVTSGPGIPIGIGFMGDLWSEEKLIGLAYAFEQ 573

BLAST of Spg001424 vs. ExPASy Swiss-Prot
Match: Q9URY4 (Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.01 PE=3 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 4.5e-67
Identity = 159/355 (44.79%), Postives = 215/355 (60.56%), Query Frame = 0

Query: 32  SIKEATLKDLQLAFKQNKLTSRQLVEFYIKQIRRYNPLLKGVIEVNPDALYLADNADRER 91
           ++++AT+  LQ   +   LTS  +V  Y+ +  + NP + G++++NPD L +A   D ER
Sbjct: 65  TLEDATIDQLQNYMENGILTSTDIVHCYLDRYLQVNPYVNGILQLNPDVLTIASELDDER 124

Query: 92  KAKAPG-SRSLLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTRLRKAGAII 151
              A G  R  LHGIP +VKDN ATKDK++TTAGS+ALLGS+VPRDA VV +LR+AGA++
Sbjct: 125 ---ANGIIRGPLHGIPFIVKDNFATKDKMDTTAGSYALLGSIVPRDAYVVKQLREAGAVL 184

Query: 152 LGKASLSEWAYFRSYGAPSGWSARAGHGREPYTLG-DPCGSSSGSAISVSANMAAVTLGT 211
            G A+LSEWA  RS     G+SAR G  R P+ L  +P GSSSGSAISV++NM A  LGT
Sbjct: 185 FGHATLSEWADMRSNDYSEGYSARGGQSRCPFNLTVNPGGSSSGSAISVASNMIAFALGT 244

Query: 212 ETDGSILCPSSQNSVVGIKPTIGLTSRAGVVPISPRQDTVGPICRTVSDAAYVLDVIVGT 271
           ETDGSI+ P+ +N VVG+KPT+GLTSR GV+P S  QDT GPI RTV DA YV   + G 
Sbjct: 245 ETDGSIIDPAMRNGVVGLKPTVGLTSRYGVIPESEHQDTTGPIARTVRDAVYVFQSMWGI 304

Query: 272 DALDDETYKASRYIPR-GGYGQFL-RANGLKGKRLGIV-EDLFSVTEPFLIQAFEEIFTI 331
           D  D  T   +   P  G Y +FL     L+G R G+  + L+   +   I    E+   
Sbjct: 305 DENDIYTLNQTGKTPEDGDYMKFLSNKTSLEGARFGLPWKRLWQNAKADEIDRLLEVVKQ 364

Query: 332 LRKRGAILVNNLKIDNLGIIFSAESGEDIALLNE---------FKEKIKEYRQDL 373
           + + GAI+ NN    NL +I +     ++  +NE         F   IK Y  ++
Sbjct: 365 IEEAGAIVYNNTNFYNLDVISNDGWNWELGSVNESEYTVVKVDFYNNIKSYLSEV 416

BLAST of Spg001424 vs. ExPASy Swiss-Prot
Match: B0K3S3 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter sp. (strain X514) OX=399726 GN=gatA PE=3 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 3.6e-40
Identity = 150/492 (30.49%), Postives = 241/492 (48.98%), Query Frame = 0

Query: 37  TLKDLQLAFKQNKLTSRQLVEFYIKQIRRYNPLLKGVIEVNPD-ALYLADNADRERKAKA 96
           T+ +L+   K+ ++++ ++ + Y+++I+   P +  ++ +  D AL  A  AD + K   
Sbjct: 7   TIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALVTITEDFALQKAKEADEKIK--- 66

Query: 97  PGSRSLLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTRLRKAGAIILGKAS 156
            G  + L GIPV++KDNI+T + + TT  S  L   + P +A VV +L + G IILGK++
Sbjct: 67  KGEDTALTGIPVIIKDNIST-EGIKTTCSSKMLENYIPPYNATVVEKLLEEGVIILGKSN 126

Query: 157 LSEWAYFRSYGAPSGWSARAGHGREPYTLGD-PCGSSSGSAISVSANMAAVTLGTETDGS 216
           L E+A        S  ++     + P+ L   P GSS GSA +++A+ AA  LG++T GS
Sbjct: 127 LDEFAM-----GSSTENSAFKTTKNPWDLSRVPGGSSGGSAAAIAADEAAFALGSDTGGS 186

Query: 217 ILCPSSQNSVVGIKPTIGLTSRAGVVPISPRQDTVGPICRTVSDAAYVLDVIVGTDALDD 276
           I  P+S   VVG+KPT GL SR G+V  +   D +GP  + V+D A VL+ I+G D  D 
Sbjct: 187 IRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIGHDPKDS 246

Query: 277 ETYKASRYIPRGGYGQFLRANGLKGKRLGIVEDLFSV-TEPFLIQAFEEIFTILRKRGAI 336
            + K    I +  Y  +L+ + +KG R+G+ ++ F    E  + +  +E   +L+  GA 
Sbjct: 247 TSLK----IDKPDYTSYLKED-IKGLRIGVAKEFFGEGIEEGVKETVQESIKVLQDLGAE 306

Query: 337 LVN------NLKIDNLGIIFSAESGEDIALLNEFK-EKIKEYRQDL--FLQAEKTKGIGM 396
           +++         +    II SAE+  ++A  +  +   I E  +DL       +++G G 
Sbjct: 307 IIDISIPYVEYALPAYYIIASAEASSNLARYDGIRYGHIAEKYEDLIDMYMVTRSEGFGK 366

Query: 397 EA---------VLS------------KLAKLSKDGLEKTITENKLDAIVTPDS------- 456
           E           LS            K+  L K+  EK     K D I+ P S       
Sbjct: 367 EVKRRIMLGTYALSSGYYDAYYKKALKVRTLIKNDFEKAF--EKCDVIIGPTSPTVAFKI 426

Query: 457 ------SISTLLA--------IGGFPGITVPAGYLPKGTPFGIHFGGLKGFEPKLIEIAY 475
                  ++  LA        I G PGI++P G L  G P G+   G    E K++ +AY
Sbjct: 427 GERANDPLAMYLADIYTVSVNIAGLPGISIPCG-LSDGLPVGLQIIGRHFDEGKILNVAY 481

BLAST of Spg001424 vs. ExPASy Swiss-Prot
Match: B0KBN4 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=gatA PE=3 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 1.0e-39
Identity = 150/492 (30.49%), Postives = 244/492 (49.59%), Query Frame = 0

Query: 37  TLKDLQLAFKQNKLTSRQLVEFYIKQIRRYNPLLKGVIEVNPD-ALYLADNADRERKAKA 96
           T+ +L+   K+ ++++ ++ + Y+++I+   P +  +I +  D AL  A  AD + K   
Sbjct: 7   TIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALITITEDFALQRAKEADEKIK--- 66

Query: 97  PGSRSLLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTRLRKAGAIILGKAS 156
            G  + L GIPV++KDNI+T + + TT  S  L   + P +A VV +L + G IILGK++
Sbjct: 67  NGEDTALTGIPVIIKDNIST-EGIKTTCSSKMLENYIPPYNATVVEKLLEEGVIILGKSN 126

Query: 157 LSEWAYFRSYGAPSGWSARAGHGREPYTLGD-PCGSSSGSAISVSANMAAVTLGTETDGS 216
           L E+A        S  ++     + P+ L   P GSS GSA +++A+ AA  LG++T GS
Sbjct: 127 LDEFAM-----GSSTENSAFKTTKNPWDLSRVPGGSSGGSAAAIAADEAAFALGSDTGGS 186

Query: 217 ILCPSSQNSVVGIKPTIGLTSRAGVVPISPRQDTVGPICRTVSDAAYVLDVIVGTDALDD 276
           I  P+S   VVG+KPT GL SR G+V  +   D +GP  + V+D A VL+ I+G D  D 
Sbjct: 187 IRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIGHDPKDS 246

Query: 277 ETYKASRYIPRGGYGQFLRANGLKGKRLGIVEDLFSV-TEPFLIQAFEEIFTILRKRGAI 336
            + K    I +  Y  +L+ + +KG R+G+ ++ F    E  + +  +E   +L+  GA 
Sbjct: 247 TSLK----IDKPDYTSYLKED-IKGLRIGVAKEFFGEGIEEGVKETVQESIKVLQDLGAE 306

Query: 337 LVN------NLKIDNLGIIFSAESGEDIA---------LLNEFKEKIKEY---RQDLFLQ 396
           +++         +    II SAE+  ++A         +  ++++ I  Y   R + F +
Sbjct: 307 IIDISIPYVEYALPAYYIIASAEASSNLARYDGIRYGHIAGKYEDLIDMYMVTRSEGFGK 366

Query: 397 AEKTK-GIGMEAVLS-----------KLAKLSKDGLEKTITENKLDAIVTPDS------- 456
             K +  +G  A+ S           K+  L K+  EK     K D I+ P S       
Sbjct: 367 EVKRRIMLGTYALSSGYYDAYYKKALKVRTLIKNDFEKAF--EKCDVIIGPTSPTVAFKI 426

Query: 457 ------SISTLLA--------IGGFPGITVPAGYLPKGTPFGIHFGGLKGFEPKLIEIAY 475
                  ++  LA        I G PGI++P G L  G P G+   G    E K++ +AY
Sbjct: 427 GERANDPLAMYLADIYTVSVNIAGLPGISIPCG-LSDGLPVGLQIIGRHFDEGKILNVAY 481

BLAST of Spg001424 vs. ExPASy TrEMBL
Match: A0A6J1CXI8 (putative amidase C869.01 OS=Momordica charantia OX=3673 GN=LOC111015494 PE=4 SV=1)

HSP 1 Score: 743.0 bits (1917), Expect = 7.6e-211
Identity = 401/510 (78.63%), Postives = 426/510 (83.53%), Query Frame = 0

Query: 1   METQSFSLSFSLFLILVAVSPFGSYSA-GTGFSIKEATLKDLQLAFKQNKLTSRQLVEFY 60
           MET   SLSFSLFLILVAVSPF SYS    GF I+EA LKDL LAFKQNKLTSRQLVEFY
Sbjct: 1   METPRLSLSFSLFLILVAVSPFWSYSVRPAGFPIEEAGLKDLHLAFKQNKLTSRQLVEFY 60

Query: 61  IKQIRRYNPLLKGVIEVNPDALYLADNADRERKAKAPGSRSLLHGIPVLVKDNIATKDKL 120
           IKQIRRYNP L+GVIEVNPDALYLAD ADRERKAKAPGS  LLHGIPVLVKDN+ATKDKL
Sbjct: 61  IKQIRRYNPRLRGVIEVNPDALYLADKADRERKAKAPGSLPLLHGIPVLVKDNMATKDKL 120

Query: 121 NTTAGSFALLGSVVPRDAGVVTRLRKAGAIILGKASLSEWAYFRSYGAPSGWSARAGHGR 180
           NTTAGS ALLGSVVPRDAG V RLRKAGAIILGKAS+SEWA FRS GAP+GW+ARAG GR
Sbjct: 121 NTTAGSLALLGSVVPRDAGAVMRLRKAGAIILGKASMSEWAGFRSNGAPTGWNARAGQGR 180

Query: 181 EPYTLGDPCGSSSGSAISVSANMAAVTLGTETDGSILCPSSQNSVVGIKPTIGLTSRAGV 240
           EPYTLG PCGSSSGSAISVSANMAAV LGTETDGSILCPSS NSVVGIKPT+GLTS AGV
Sbjct: 181 EPYTLGLPCGSSSGSAISVSANMAAVALGTETDGSILCPSSFNSVVGIKPTLGLTSTAGV 240

Query: 241 VPISPRQDTVGPICRTVSDAAYVLDVIVGTDALDDETYKASRYIPRGGYGQFLRANGLKG 300
           VPISPRQDTVGPICRTVSDAAYVLDVIVGTD LD+ TYKASRYIPRGGYGQFL+A+GL+G
Sbjct: 241 VPISPRQDTVGPICRTVSDAAYVLDVIVGTDPLDNSTYKASRYIPRGGYGQFLKADGLRG 300

Query: 301 KRLGIVEDLFSVTEPFLIQAFEEIFTILRKRGAILVNNLKIDNLGIIF-SAESGEDIALL 360
           KRLGIVED F V +P LI AFEEIFTIL KRGAILV+NLKIDNLG I+ + ESGE  ALL
Sbjct: 301 KRLGIVEDFFGVVDPSLIPAFEEIFTILSKRGAILVDNLKIDNLGAIYNNTESGEVAALL 360

Query: 361 NEFK-------------------------------EKIKEYRQDLFLQAEKTKGIGMEAV 420
           NEFK                               EKIKEY QDLFLQAE T G+G+  +
Sbjct: 361 NEFKVSLNAYLKELVSSPIRSLAEAIAFNQKHSSLEKIKEYGQDLFLQAEATNGMGINGL 420

Query: 421 LSKLAKLSKDGLEKTITENKLDAIVTPDSSISTLLAIGGFPGITVPAGYLPKGTPFGIHF 478
           LSKL KLSKDGLEKT+ +NKLDAI+TP + IS +LAIGGFPGITVPAGY P GTPFGI F
Sbjct: 421 LSKLGKLSKDGLEKTMIKNKLDAIMTPSAIISPILAIGGFPGITVPAGYSPSGTPFGIDF 480

BLAST of Spg001424 vs. ExPASy TrEMBL
Match: A0A0A0LM97 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264020 PE=4 SV=1)

HSP 1 Score: 614.4 bits (1583), Expect = 4.1e-172
Identity = 334/512 (65.23%), Postives = 386/512 (75.39%), Query Frame = 0

Query: 1   METQSFSLSFSLFLILVAV-SPFGSYSAGTGFSIKEATLKDLQLAFKQNKLTSRQLVEFY 60
           M  QSF L  S+ L L+A+ S  GS S  T  S++EATLKDLQ AF QNKLTSRQLVEFY
Sbjct: 1   MAGQSFLLDISMLLGLMAILSSCGSCSFHTELSLEEATLKDLQRAFYQNKLTSRQLVEFY 60

Query: 61  IKQIRRYNPLLKGVIEVNPDALYLADNADRERKAKAPGSRSLLHGIPVLVKDNIATKDKL 120
           ++Q+RR NP+LKG+IEVNPDAL  A  AD ERK  +P S S LHGIPVLVKDNIATKDKL
Sbjct: 61  LEQVRRLNPILKGIIEVNPDALDQASRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 121 NTTAGSFALLGSVVPRDAGVVTRLRKAGAIILGKASLSEWAYFRSYGAPSGWSARAGHGR 180
           NTTAGSFALLGSVVPRDAGVVT+LR AGAII GKASLSEW+YFRS   PSGWSAR G G+
Sbjct: 121 NTTAGSFALLGSVVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNALPSGWSARGGQGK 180

Query: 181 EPYTLGDPCGSSSGSAISVSANMAAVTLGTETDGSILCPSSQNSVVGIKPTIGLTSRAGV 240
            PYT+G+PCGSSSGSAISV+ANM  V+LGTETDGSILCPS+ NSVVGIKPT+GLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 VPISPRQDTVGPICRTVSDAAYVLDVIVGTDALDDETYKASRYIPRGGYGQFLRANGLKG 300
           VPIS RQDTVGPICRTVSDAAYVL+ IVG D  D+ T +AS+YIP+GGYGQFLRA GLKG
Sbjct: 241 VPISSRQDTVGPICRTVSDAAYVLEAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 301 KRLGIVEDL--FSVTEPFLIQAFEEIFTILRKRGAILVNNLKIDNLGIIFSAESGEDIAL 360
           KR+GIV +   F   + F  QA+E++   L+K GAILV+NL IDNL  IF   SGE IAL
Sbjct: 301 KRIGIVREFYDFGPDDTFYTQAYEKVVKTLKKGGAILVDNLMIDNLEQIFDGSSGEQIAL 360

Query: 361 LNEFKEKIK------------------------------EYRQDLFLQAEKTKGIG--ME 420
           L EFK  +                               EY Q+ FL+AE T GIG   +
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQEEFLKAEATNGIGDAEK 420

Query: 421 AVLSKLAKLSKDGLEKTITENKLDAIVTPDSSISTLLAIGGFPGITVPAGYLPKGTPFGI 478
           A L++LAKLSKDG E+ + +NKLDA+  P S IS + AIGGFPG++VPAGY P+G P+GI
Sbjct: 421 AALARLAKLSKDGFERLMIKNKLDAVAAPGSLISPVFAIGGFPGVSVPAGYDPQGNPYGI 480

BLAST of Spg001424 vs. ExPASy TrEMBL
Match: A0A5D3BKT1 (Putative amidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001390 PE=4 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 4.5e-171
Identity = 332/511 (64.97%), Postives = 385/511 (75.34%), Query Frame = 0

Query: 1   METQSFSLSFSLFLILVAV-SPFGSYSAGTGFSIKEATLKDLQLAFKQNKLTSRQLVEFY 60
           M  QSF L  S+ L L+A+ S +GS S  T  SI+EATL DLQ AF QNKLTSRQLVEFY
Sbjct: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60

Query: 61  IKQIRRYNPLLKGVIEVNPDALYLADNADRERKAKAPGSRSLLHGIPVLVKDNIATKDKL 120
           ++Q+RR+NP+LKG+IEVNPDAL  A  AD ERK  +P S S LHGIPVLVKDNIATKDKL
Sbjct: 61  LEQVRRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 121 NTTAGSFALLGSVVPRDAGVVTRLRKAGAIILGKASLSEWAYFRSYGAPSGWSARAGHGR 180
           NTTAGSFALLGS+VPRDAGVVT+LR AGAII GKASLSEW+YFRS   PSGWSAR G G+
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180

Query: 181 EPYTLGDPCGSSSGSAISVSANMAAVTLGTETDGSILCPSSQNSVVGIKPTIGLTSRAGV 240
            PY LG+PCGSSSGSAISV+ANM  V+LGTETDGSILCPS+ NSVVGIKPT+GLTSRAGV
Sbjct: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 VPISPRQDTVGPICRTVSDAAYVLDVIVGTDALDDETYKASRYIPRGGYGQFLRANGLKG 300
           +PIS RQD+VGPICRTVSDA YVLD IVG D  D+ T +AS+YIP+GGYGQFLRA GLKG
Sbjct: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 301 KRLGIVEDL--FSVTEPFLIQAFEEIFTILRKRGAILVNNLKIDNLGIIFSAESGEDIAL 360
           KR+GIV +   F   E F  QA+E++   L+K GAILV+N  IDNL +IF   SGE IAL
Sbjct: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360

Query: 361 LNEFKEKIK------------------------------EYRQDLFLQAEKTKGIG--ME 420
           L EFK  +                               EY Q+LFL+AE T GIG   +
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQELFLKAEATNGIGDAEK 420

Query: 421 AVLSKLAKLSKDGLEKTITENKLDAIVTPDSSISTLLAIGGFPGITVPAGYLPKGTPFGI 477
           A L++LAKLSKDG E+ + +NKLDAI  P S IS++ AIGGFPG++VPAGY P+G P+GI
Sbjct: 421 AALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGVSVPAGYDPQGVPYGI 480

BLAST of Spg001424 vs. ExPASy TrEMBL
Match: E5GC09 (Amidase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 4.5e-171
Identity = 332/511 (64.97%), Postives = 385/511 (75.34%), Query Frame = 0

Query: 1   METQSFSLSFSLFLILVAV-SPFGSYSAGTGFSIKEATLKDLQLAFKQNKLTSRQLVEFY 60
           M  QSF L  S+ L L+A+ S +GS S  T  SI+EATL DLQ AF QNKLTSRQLVEFY
Sbjct: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60

Query: 61  IKQIRRYNPLLKGVIEVNPDALYLADNADRERKAKAPGSRSLLHGIPVLVKDNIATKDKL 120
           ++Q+RR+NP+LKG+IEVNPDAL  A  AD ERK  +P S S LHGIPVLVKDNIATKDKL
Sbjct: 61  LEQVRRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 121 NTTAGSFALLGSVVPRDAGVVTRLRKAGAIILGKASLSEWAYFRSYGAPSGWSARAGHGR 180
           NTTAGSFALLGS+VPRDAGVVT+LR AGAII GKASLSEW+YFRS   PSGWSAR G G+
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180

Query: 181 EPYTLGDPCGSSSGSAISVSANMAAVTLGTETDGSILCPSSQNSVVGIKPTIGLTSRAGV 240
            PY LG+PCGSSSGSAISV+ANM  V+LGTETDGSILCPS+ NSVVGIKPT+GLTSRAGV
Sbjct: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 VPISPRQDTVGPICRTVSDAAYVLDVIVGTDALDDETYKASRYIPRGGYGQFLRANGLKG 300
           +PIS RQD+VGPICRTVSDA YVLD IVG D  D+ T +AS+YIP+GGYGQFLRA GLKG
Sbjct: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 301 KRLGIVEDL--FSVTEPFLIQAFEEIFTILRKRGAILVNNLKIDNLGIIFSAESGEDIAL 360
           KR+GIV +   F   E F  QA+E++   L+K GAILV+N  IDNL +IF   SGE IAL
Sbjct: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360

Query: 361 LNEFKEKIK------------------------------EYRQDLFLQAEKTKGIG--ME 420
           L EFK  +                               EY Q+LFL+AE T GIG   +
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQELFLKAEATNGIGDAEK 420

Query: 421 AVLSKLAKLSKDGLEKTITENKLDAIVTPDSSISTLLAIGGFPGITVPAGYLPKGTPFGI 477
           A L++LAKLSKDG E+ + +NKLDAI  P S IS++ AIGGFPG++VPAGY P+G P+GI
Sbjct: 421 AALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGVSVPAGYDPQGVPYGI 480

BLAST of Spg001424 vs. ExPASy TrEMBL
Match: A0A1S3C5K8 (putative amidase C869.01 OS=Cucumis melo OX=3656 GN=LOC103497307 PE=4 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 4.5e-171
Identity = 332/511 (64.97%), Postives = 385/511 (75.34%), Query Frame = 0

Query: 1   METQSFSLSFSLFLILVAV-SPFGSYSAGTGFSIKEATLKDLQLAFKQNKLTSRQLVEFY 60
           M  QSF L  S+ L L+A+ S +GS S  T  SI+EATL DLQ AF QNKLTSRQLVEFY
Sbjct: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFYQNKLTSRQLVEFY 60

Query: 61  IKQIRRYNPLLKGVIEVNPDALYLADNADRERKAKAPGSRSLLHGIPVLVKDNIATKDKL 120
           ++Q+RR+NP+LKG+IEVNPDAL  A  AD ERK  +P S S LHGIPVLVKDNIATKDKL
Sbjct: 61  LEQVRRFNPILKGIIEVNPDALDQASRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKL 120

Query: 121 NTTAGSFALLGSVVPRDAGVVTRLRKAGAIILGKASLSEWAYFRSYGAPSGWSARAGHGR 180
           NTTAGSFALLGS+VPRDAGVVT+LR AGAII GKASLSEW+YFRS   PSGWSAR G G+
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGK 180

Query: 181 EPYTLGDPCGSSSGSAISVSANMAAVTLGTETDGSILCPSSQNSVVGIKPTIGLTSRAGV 240
            PY LG+PCGSSSGSAISV+ANM  V+LGTETDGSILCPS+ NSVVGIKPT+GLTSRAGV
Sbjct: 181 NPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 VPISPRQDTVGPICRTVSDAAYVLDVIVGTDALDDETYKASRYIPRGGYGQFLRANGLKG 300
           +PIS RQD+VGPICRTVSDA YVLD IVG D  D+ T +AS+YIP+GGYGQFLRA GLKG
Sbjct: 241 IPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKG 300

Query: 301 KRLGIVEDL--FSVTEPFLIQAFEEIFTILRKRGAILVNNLKIDNLGIIFSAESGEDIAL 360
           KR+GIV +   F   E F  QA+E++   L+K GAILV+N  IDNL +IF   SGE IAL
Sbjct: 301 KRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIAL 360

Query: 361 LNEFKEKIK------------------------------EYRQDLFLQAEKTKGIG--ME 420
           L EFK  +                               EY Q+LFL+AE T GIG   +
Sbjct: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENLEYGQELFLKAEATNGIGDAEK 420

Query: 421 AVLSKLAKLSKDGLEKTITENKLDAIVTPDSSISTLLAIGGFPGITVPAGYLPKGTPFGI 477
           A L++LAKLSKDG E+ + +NKLDAI  P S IS++ AIGGFPG++VPAGY P+G P+GI
Sbjct: 421 AALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGVSVPAGYDPQGVPYGI 480

BLAST of Spg001424 vs. TAIR 10
Match: AT4G34880.1 (Amidase family protein )

HSP 1 Score: 434.1 bits (1115), Expect = 1.4e-121
Identity = 256/507 (50.49%), Postives = 321/507 (63.31%), Query Frame = 0

Query: 10  FSLFLILVAVSPFGSYSAGTG--------FSIKEATLKDLQLAFKQNKLTSRQLVEFYIK 69
           FS  LIL+ +S     S G+         FSI+EAT++D+++AF + +LTS+QLVE Y++
Sbjct: 9   FSSLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLE 68

Query: 70  QIRRYNPLLKGVIEVNPDALYLADNADRERKAKAPGSRSLLHGIPVLVKDNIATKDKLNT 129
            I + NP+L  VIE NPDAL  A+ ADRER  K      +LHG+PVL+KD+I+TKDKLNT
Sbjct: 69  AISKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDKLNT 128

Query: 130 TAGSFALLGSVVPRDAGVVTRLRKAGAIILGKASLSEWAYFRSYGAPSGWSARAGHGREP 189
           TAGSFALLGSVV RDAGVV RLR++GA+ILGKASLSEWA+FRS+  P GWSA        
Sbjct: 129 TAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSA-------- 188

Query: 190 YTLGDPCGSSSGSAISVSANMAAVTLGTETDGSILCPSSQNSVVGIKPTIGLTSRAGVVP 249
                                                 SQNSVVGIKP++GLTSRAGVVP
Sbjct: 189 -------------------------------------PSQNSVVGIKPSVGLTSRAGVVP 248

Query: 250 ISPRQDTVGPICRTVSDAAYVLDVIVGTDALDDETYKASRYIPRGGYGQFLRANGLKGKR 309
           IS RQD++GPICRTVSDA ++LD IVG D LD+ T  AS +IP GGY QFL  +GLKGKR
Sbjct: 249 ISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLTTSGLKGKR 308

Query: 310 LGIVEDLFSVTEPFLIQAFEEIFTILRKRGAILVNNLKIDNLGIIF-SAESGEDIALLNE 369
           LGIV    S+ +  +          LR+ GAI++NNL I N+ +I    +SGE+IALL E
Sbjct: 309 LGIVMKHSSLLDHHI--------KTLRREGAIVINNLTIPNIEVIVGGTDSGEEIALLAE 368

Query: 370 FK-------------------------------EKIKEYRQDLFLQAEKTKGIG--MEAV 429
           FK                               EK+KE+ Q++FL AE T G+G   +  
Sbjct: 369 FKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKEKTA 428

Query: 430 LSKLAKLSKDGLEKTITENKLDAIVTPDSSISTLLAIGGFPGITVPAGYLPKGTPFGIHF 475
           L K+ +LS++G+EK I ENKLDAIVT  S +S++LAIGG+PGI VPAGY   G P+GI F
Sbjct: 429 LQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYGISF 462

BLAST of Spg001424 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 115.5 bits (288), Expect = 1.1e-25
Identity = 79/233 (33.91%), Postives = 119/233 (51.07%), Query Frame = 0

Query: 46  KQNKLTSRQLVEFYIKQIRRYNPLLKGVIEVNPDALYL----ADNADRERKAKAPGSRSL 105
           K  ++TS++LV  Y+KQ++RYN +L+ V+    +  Y     AD+   +     P     
Sbjct: 213 KTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEADDLLSQGTYLGP----- 272

Query: 106 LHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTRLRKAGAIILGKASLSEWAY 165
           LHGIP  +KD +A      TT GS +     +  +A V  RL+ +GA+++ K      AY
Sbjct: 273 LHGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTGSMAY 332

Query: 166 FRSYGAPSGWSARAGHGREPYTLGD-PCGSSSGSAISVSANMAAVTLGTETDGSILCPSS 225
                    W    G  R P+ + +   GSS+G A S SA M    +G+ET GS+  P++
Sbjct: 333 ------DDIWF--GGRTRNPWNIEEFSTGSSAGPAASTSAGMVPFAIGSETAGSMTYPAA 392

Query: 226 QNSVVGIKPTIGLTSRAGVVPISPRQDTVGPICRTVSDAAYVLDVIVGTDALD 274
           +  +  ++PT G   R GV+ IS   D +GP CRT +D A +LD I G D  D
Sbjct: 393 RCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDD 431

BLAST of Spg001424 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 113.6 bits (283), Expect = 4.3e-25
Identity = 137/486 (28.19%), Postives = 216/486 (44.44%), Query Frame = 0

Query: 51  TSRQLVEFYIKQIRRYNPLLKGVIEVNPDALYLADNADRERKAKAPGSRSLLHGIPVLVK 110
           T+ ++ + Y+ +IR   P LK  + V+ + L  A   D +R AK       L G+ + VK
Sbjct: 59  TAVEIAKSYLSRIRLTEPQLKCFLHVSENVLKDAQEID-QRIAKGE-ELGPLAGVLIGVK 118

Query: 111 DNIATKDKLNTTAGSFALLGSVVPRDAGVVTRLRKAGAIILGKASLSEWAYFRSYGAPSG 170
           DNI T+  + +TA S  L     P DA  V ++++ G I++GK ++ E      +G  S 
Sbjct: 119 DNICTQG-MPSTAASRILEHYRPPFDATAVKKIKELGGIVVGKTNMDE------FGMGST 178

Query: 171 WSARAGH-GREPYTLGD-PCGSSSGSAISVSANMAAVTLGTETDGSILCPSSQNSVVGIK 230
             A A      P+ L   P GSS GSA +V+A    V+LG++T GS+  P+S   VVG+K
Sbjct: 179 TEASAFQVTANPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCGVVGLK 238

Query: 231 PTIGLTSRAGVVPISPRQDTVGPICRTVSDAAYVLDVIVGTDALDDETYKASRYIPRGGY 290
           PT G  SR G++  +   D +G    TV+DA  +L  I G D  D  + K    +P    
Sbjct: 239 PTYGRVSRFGLMAYASSLDVIGCFGSTVADAGMLLHAISGYDRFDSTSSKQD--VPE-FQ 298

Query: 291 GQFL-----RANGLKGKRLGIV-EDLFSVTEPFLIQAFEEIFTILRKRGAILVN-NLKID 350
            QFL      +  L G ++GI+ E L    +  +  A +E  + L   G IL   +L   
Sbjct: 299 SQFLSVDHFESKPLNGVKVGIIRETLEDGVDSGVRSATQEAASHLEALGCILTEVSLPSF 358

Query: 351 NLG-----IIFSAESGEDIALLNEFKEKIKEYRQDLFLQAEKTKGIGM------------ 410
           +LG     +I S+ES  +++  +  +   +   ++L    E ++G G             
Sbjct: 359 SLGLPAYYVIASSESSSNLSRYDGVRYGNQVMAEELNKLYECSRGEGFGGEVKMRILMGT 418

Query: 411 ---------------EAVLSKLAKLSKDGLEKT--------------ITENKLDAIVTPD 470
                          + V + + K  K  LE+               I E K D +    
Sbjct: 419 YALSAGYYDAYYKRAQQVRTLIRKDFKAALEQNDILISPAAPSAAYKIGEKKDDPLAMYA 478

Query: 471 SSISTL-LAIGGFPGITVPAGYL---PKGTPFGIHFGGLKGFEPKLIEIAYDFEQATKRR 477
             I T+ + + G P + +P G +   P G P G+   G    E KL+++ + FEQ  K  
Sbjct: 479 GDIMTVNVNLAGLPAMVLPCGLVEGGPSGLPVGLQMIGAAFDEEKLLKVGHIFEQTLKGS 532

BLAST of Spg001424 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 107.1 bits (266), Expect = 4.1e-23
Identity = 78/233 (33.48%), Postives = 117/233 (50.21%), Query Frame = 0

Query: 46  KQNKLTSRQLVEFYIKQIRRYNPLLKGVIEVNPDALYL----ADNADRERKAKAPGSRSL 105
           K  ++TS++LV  Y+KQ++RYN +L+ V+    +  Y     AD+   +     P     
Sbjct: 213 KTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEADDLLSQGTYLGP----- 272

Query: 106 LHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTRLRKAGAIILGKASLSEWAY 165
           LHGIP  +KD +A      TT GS +     +  +A V  RL+ +GA+++ K      AY
Sbjct: 273 LHGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTGSMAY 332

Query: 166 FRSYGAPSGWSARAGHGREPYTLGD-PCGSSSGSAISVSANMAAVTLGTETDGSILCPSS 225
                    W    G  R P+ + +   GSS+G A S SA       G+ET GS+  P++
Sbjct: 333 ------DDIWF--GGRTRNPWNIEEFSTGSSAGPAASTSA-------GSETAGSMTYPAA 392

Query: 226 QNSVVGIKPTIGLTSRAGVVPISPRQDTVGPICRTVSDAAYVLDVIVGTDALD 274
           +  +  ++PT G   R GV+ IS   D +GP CRT +D A +LD I G D  D
Sbjct: 393 RCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDD 424

BLAST of Spg001424 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 90.5 bits (223), Expect = 3.9e-18
Identity = 119/506 (23.52%), Postives = 198/506 (39.13%), Query Frame = 0

Query: 21  PFGSYSAGTGFSIKEATLKDLQLAFKQNKLTSRQLVEFYIKQIRRY---NPLLKGVIEVN 80
           P  S  A    S +   ++D   A++    T  Q+ +  I  I  +    P    +I  +
Sbjct: 114 PSRSLHADPVSSFRYWKIRDYAYAYRSKLTTPLQVAKRIISIIEEFGYDKPPTPFLIRFD 173

Query: 81  PDALYLADNADRERKAKAPGSRSLLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDA 140
            + +     A   R+ +     S+L GI V +KD+I           ++      V +D+
Sbjct: 174 ANEVIKQAEAS-TRRFEQGNPISVLDGIFVTIKDDIDCLPHPTNGGTTWLHEDRSVEKDS 233

Query: 141 GVVTRLRKAGAIILGKASLSEWAYFRSYGAPSGWSARAGHGREPYTLGDPCGSSSGSAIS 200
            VV++LR  GAI+LGKA++ E     +    +  + R  H  + YT     GSSSGSA  
Sbjct: 234 AVVSKLRSCGAILLGKANMHELGMGTTGNNSNYGTTRNPHDPKRYT----GGSSSGSAAI 293

Query: 201 VSANMAAVTLGTETDGSILCPSSQNSVVGIKPTIGLTSRAGVVPISPRQDTVGPICRTVS 260
           V+A + +  LGT+  GS+  PS+   + G+K T G T   G +      + +GP+  ++ 
Sbjct: 294 VAAGLCSAALGTDGGGSVRIPSALCGITGLKTTYGRTDMTGSLCEGGTVEIIGPLASSLE 353

Query: 261 DAAYVLDVIVGTDALDDETYKAS-----RYIPRGGYGQFLRANGLKGKRLGIVEDLFS-V 320
           DA  V   I+G+ + D    K S     + +   G      +N +   RLG     F+ V
Sbjct: 354 DAFLVYAAILGSSSADRYNLKPSPPCFPKLLSHNG------SNAIGSLRLGKYTKWFNDV 413

Query: 321 TEPFLIQAFEEIFTILRKRGAILVNNLKIDNLG--------------------------- 380
           +   +    E+I  +L       V  + +  L                            
Sbjct: 414 SSSDISDKCEDILKLLSNNHGCKVVEIVVPELEEMRAAHVISIGSPTLSSLTPYCEAGKN 473

Query: 381 -----------IIFSAESGEDIALLNEFKEKIKEYRQDLFLQAE--KTKGIGMEAVLSKL 440
                       IF + S  D       + ++ EY  ++F   +   T   GM A +   
Sbjct: 474 SKLSYDTRTSFAIFRSFSASDYIAAQCLRRRLMEYHLNIFKDVDVIVTPTTGMTAPV--- 533

Query: 441 AKLSKDGLEKTITENKLDAIVTPDSSISTLLA-IGGFPGITVPAGYLPKGTPFGIHFGGL 477
             +  D L+   T  +    VT D     L A + GFP I+VP GY  +G P G+   G 
Sbjct: 534 --IPPDALKNGETNIQ----VTTDLMRFVLAANLLGFPAISVPVGYDKEGLPIGLQIMGR 593