Spg001276 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg001276
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionGlutamyl-tRNA(Gln) amidotransferase subunit A
Locationscaffold8: 38814153 .. 38817006 (+)
RNA-Seq ExpressionSpg001276
SyntenySpg001276
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTACTTTTTCTTGGTCCATAGAATGGAACCTAAAAGCTTATCTCCTTACCTTCACTTGCTTCTGATTTTGGTTGCAGTATCGGCAATTGAGTCTGGAAGCGTGGGAACTGGCTTCTCCATTGAAGAAGCCACGTTGAAAGATTTGCGATCGGCCTTCTTCAACCAAACAAATCTCACTTCTAGACGGCTCGTGGAGTTTTACATCGAGCAAATCCGTAAATACAATGCAAGTCTTGGAGGAGTGATTGAAGTGAATCCAGATGCACTAGACTTGGCCGACGCTGCCGATCGCAAGCGCAGGGCGAGCACGACGCCGAGCGCGCTGCCTCTGTTGCACGGCATTCCTGTGCTTGTCAAAGATAATATAGCAACAAAGGACAAGCTCAACACCACTGCGGGCTCGTTTGCTTTGTTGAGCTCCGTTGTGGCTCGTGATGCAGGGGTCGTGACCAAGTTGAGGAAAGCTGGAGCTATCATATTGGGAAAGGCCAGCTTGAGCCAGTGGTCTGGTTTTAGATCTTCCAACGCTCCTAGTGGTTGGAATGCTAGGAGTGGTCAAGGCCGTGTGAGTTTCCCTCCAATTTCTCCGATATTCATCTTACTTGTTTTATTATATTCCTTAAAATGATGACATAATCAATTAGTCATCTATACTTTAATTAAAAATTCAGATTGCAACATTTAACAAATTAGAGGAGCTAATTGATCAAATTAAAAGTTAAGGTCACGTTTAATAACCATTTGATTTTTTGTTTTTCGTTTTTGAAAATTATGCTTGTTTTCTCATAAATTTCCTACCATAACTTTCATCCTTGTTAAGGATCCATTTGAATTTCTAGCCAAATTCTAAAAACAAAAACAAATTTTTGAAAACTATTATTTTTTGTTTTCAAAATTGGACTTGGTTTTTGAAAACAATGAAGAAGATGGATACTAAAACAAAGAAACTCATAGGTGGAAGTTGATGTGTATAAGCTTAATTTTAAAAAACCAAATGGTTATCAAACGGGGCTTAAAGGGTGTGATTGTAACAAACCCTACAGTTCAAGAGTGATTTGTATAATTTTCCCTTGCTTTATTAACATTTGGATGACAGATACACAGTTGAATATGTTAATAAAATAATAAATGCGCACACAGGAACCGTATACATTGGGAGATCCTTGCGGTTCGAGCAGTGGCTCAGCAATATCAGTGTCAGCAAACATGGCTGCTGTCGCCATAGGAACTGAAACCGATGGCTCATTACTATGCCCTGCTGCTTATAACTCGGTGGTTGCAATCAAACCAACGGTTGGACTCACTAGTCGAGCTGGAGTTATCCCAATCTCAACCCGGCAAGATACTGTTGGGTAAACCTCTATCTCACTTAGCAGCCACATGTTTTTCTTCAATACTTCATTTAAATAATAATACATATAATTTGACTCTTGATGCATGAAACAGACCAATTGGCAGAACAGTGGCCGATGCTGCTTATGTTCTTGATGCCATTGTCGGATATGACGCCTTTGATCAGGCAACATATTGCCGTTCTCAATTCATTCCTAAAGATGGGTATGGCCAATTTCTTAAGGCCGATGGACTTCGAGGAAAGAGATTGGGAATAGTAGAAGATTTCTCTATCGGTATTCGTCCTTCTATAGTTCAAGCTTTTCAGGAAGCTTTACAAATATTAAGGTACCAAAAGGCAATTTGAATTTATCATCGGTGTATTCGATTTAGTCTCTCTTACATATATTTTTTTCAATCAGTTTAGTCCTTCATTGTTGGTGAATGTTAAAATTGACTGATGATAAACTTACCTGACAAAAAACTCAGTAAATTAGTGAACATTTTAAAAGAGAAAAAAAAATGCCATCGATGAGAAGTAAAATTTATAATCCTCCTATAATTTCCTATGAAATTTGGTGTTTTCTTCACTTGGATTGAACTTTTTCGTCAATACAATAATTATTATGTCATGTAAGTTTATCGTCAACCAGTTTTAACATTCACTACTAGTGAGAAACTGAATTAAAAAATATTCATACAAAAGAGGCTAAATCGAAACAAAACTTAAACATTAACAACTAAAACATATATATGACTAGTTTTAGCAATTTATCTAAATGTTACCTGCAGTAAAAGAGGTGCAATATTGGTGAGCAATTTGAAAATAAGAGACTTTAAAACCATAGCAAATCCTGGAGAAAGTGCGAAAGTAACTGCATTGTTGAACGAGTTCAGAGTATCTGTAAATGCATATCTAGAAGGGCTTGTTTCCTCTCCAGTTCAATCTTTATCAGGTGTTATAGAATTCAACAAGCAGCACGTACACCTAGTAAGCATTGCTTGTATTCAACTTCGGGTCTATTTGGAATGACTTTCTAAGAGTTTTTAAACTCTTAGAAAGTCATTCCAAACATACACTACAACTATATATATAGCAAAACTCACTCCGAACCATAGCGATTATTGAGATTTTGTTAACGATGAAACACAGGAGGAAATGGCAAAGTATGGTCAAGACTTCTTACTAGAAGCTAACAGGACGAATGGAATAGGACTGGGAGCAGCATTGTCAAAATTAAGGGCACTTTCAAGAGAAGGATTTGAGAAAACGATGACCGACAATAGGCTAGATGCAATAATAGCTCCAAGTGAAATAATTTGCTCATTTTTAGCAATTGGAGGTCTTCCGGGAATTACTGTTCCGGCAGGATATACGCCAACAGGAAAGCCGTTTGGGTTTTATTTTGGAGGGTTGAGAGGATACGAACCGAGGCTGATAGAGATTGCATATGACTTTGAACAAGCCACCAAAACGAGAAAGCCTCCTCCTTTGAGTAAACTGGCTCGCTCATCTCTGTAG

mRNA sequence

ATGGAACCTAAAAGCTTATCTCCTTACCTTCACTTGCTTCTGATTTTGGTTGCAGTATCGGCAATTGAGTCTGGAAGCGTGGGAACTGGCTTCTCCATTGAAGAAGCCACGTTGAAAGATTTGCGATCGGCCTTCTTCAACCAAACAAATCTCACTTCTAGACGGCTCGTGGAGTTTTACATCGAGCAAATCCGTAAATACAATGCAAGTCTTGGAGGAGTGATTGAAGTGAATCCAGATGCACTAGACTTGGCCGACGCTGCCGATCGCAAGCGCAGGGCGAGCACGACGCCGAGCGCGCTGCCTCTGTTGCACGGCATTCCTGTGCTTGTCAAAGATAATATAGCAACAAAGGACAAGCTCAACACCACTGCGGGCTCGTTTGCTTTGTTGAGCTCCGTTGTGGCTCGTGATGCAGGGGTCGTGACCAAGTTGAGGAAAGCTGGAGCTATCATATTGGGAAAGGCCAGCTTGAGCCAGTGGTCTGGTTTTAGATCTTCCAACGCTCCTAGTGGTTGGAATGCTAGGAGTGGTCAAGGCCGTGAACCGTATACATTGGGAGATCCTTGCGGTTCGAGCAGTGGCTCAGCAATATCAGTGTCAGCAAACATGGCTGCTGTCGCCATAGGAACTGAAACCGATGGCTCATTACTATGCCCTGCTGCTTATAACTCGGTGGTTGCAATCAAACCAACGGTTGGACTCACTAGTCGAGCTGGAGTTATCCCAATCTCAACCCGGCAAGATACTGTTGGACCAATTGGCAGAACAGTGGCCGATGCTGCTTATGTTCTTGATGCCATTGTCGGATATGACGCCTTTGATCAGGCAACATATTGCCGTTCTCAATTCATTCCTAAAGATGGGTATGGCCAATTTCTTAAGGCCGATGGACTTCGAGGAAAGAGATTGGGAATAGTAGAAGATTTCTCTATCGGTATTCGTCCTTCTATAGTTCAAGCTTTTCAGGAAGCTTTACAAATATTAAGTAAAAGAGGTGCAATATTGGTGAGCAATTTGAAAATAAGAGACTTTAAAACCATAGCAAATCCTGGAGAAAGTGCGAAAGTAACTGCATTGTTGAACGAGTTCAGAGTATCTGTAAATGCATATCTAGAAGGGCTTGTTTCCTCTCCAGTTCAATCTTTATCAGGTGTTATAGAATTCAACAAGCAGCACGTACACCTAGAGGAAATGGCAAAGTATGGTCAAGACTTCTTACTAGAAGCTAACAGGACGAATGGAATAGGACTGGGAGCAGCATTGTCAAAATTAAGGGCACTTTCAAGAGAAGGATTTGAGAAAACGATGACCGACAATAGGCTAGATGCAATAATAGCTCCAAGTGAAATAATTTGCTCATTTTTAGCAATTGGAGGTCTTCCGGGAATTACTGTTCCGGCAGGATATACGCCAACAGGAAAGCCGTTTGGGTTTTATTTTGGAGGGTTGAGAGGATACGAACCGAGGCTGATAGAGATTGCATATGACTTTGAACAAGCCACCAAAACGAGAAAGCCTCCTCCTTTGAGTAAACTGGCTCGCTCATCTCTGTAG

Coding sequence (CDS)

ATGGAACCTAAAAGCTTATCTCCTTACCTTCACTTGCTTCTGATTTTGGTTGCAGTATCGGCAATTGAGTCTGGAAGCGTGGGAACTGGCTTCTCCATTGAAGAAGCCACGTTGAAAGATTTGCGATCGGCCTTCTTCAACCAAACAAATCTCACTTCTAGACGGCTCGTGGAGTTTTACATCGAGCAAATCCGTAAATACAATGCAAGTCTTGGAGGAGTGATTGAAGTGAATCCAGATGCACTAGACTTGGCCGACGCTGCCGATCGCAAGCGCAGGGCGAGCACGACGCCGAGCGCGCTGCCTCTGTTGCACGGCATTCCTGTGCTTGTCAAAGATAATATAGCAACAAAGGACAAGCTCAACACCACTGCGGGCTCGTTTGCTTTGTTGAGCTCCGTTGTGGCTCGTGATGCAGGGGTCGTGACCAAGTTGAGGAAAGCTGGAGCTATCATATTGGGAAAGGCCAGCTTGAGCCAGTGGTCTGGTTTTAGATCTTCCAACGCTCCTAGTGGTTGGAATGCTAGGAGTGGTCAAGGCCGTGAACCGTATACATTGGGAGATCCTTGCGGTTCGAGCAGTGGCTCAGCAATATCAGTGTCAGCAAACATGGCTGCTGTCGCCATAGGAACTGAAACCGATGGCTCATTACTATGCCCTGCTGCTTATAACTCGGTGGTTGCAATCAAACCAACGGTTGGACTCACTAGTCGAGCTGGAGTTATCCCAATCTCAACCCGGCAAGATACTGTTGGACCAATTGGCAGAACAGTGGCCGATGCTGCTTATGTTCTTGATGCCATTGTCGGATATGACGCCTTTGATCAGGCAACATATTGCCGTTCTCAATTCATTCCTAAAGATGGGTATGGCCAATTTCTTAAGGCCGATGGACTTCGAGGAAAGAGATTGGGAATAGTAGAAGATTTCTCTATCGGTATTCGTCCTTCTATAGTTCAAGCTTTTCAGGAAGCTTTACAAATATTAAGTAAAAGAGGTGCAATATTGGTGAGCAATTTGAAAATAAGAGACTTTAAAACCATAGCAAATCCTGGAGAAAGTGCGAAAGTAACTGCATTGTTGAACGAGTTCAGAGTATCTGTAAATGCATATCTAGAAGGGCTTGTTTCCTCTCCAGTTCAATCTTTATCAGGTGTTATAGAATTCAACAAGCAGCACGTACACCTAGAGGAAATGGCAAAGTATGGTCAAGACTTCTTACTAGAAGCTAACAGGACGAATGGAATAGGACTGGGAGCAGCATTGTCAAAATTAAGGGCACTTTCAAGAGAAGGATTTGAGAAAACGATGACCGACAATAGGCTAGATGCAATAATAGCTCCAAGTGAAATAATTTGCTCATTTTTAGCAATTGGAGGTCTTCCGGGAATTACTGTTCCGGCAGGATATACGCCAACAGGAAAGCCGTTTGGGTTTTATTTTGGAGGGTTGAGAGGATACGAACCGAGGCTGATAGAGATTGCATATGACTTTGAACAAGCCACCAAAACGAGAAAGCCTCCTCCTTTGAGTAAACTGGCTCGCTCATCTCTGTAG

Protein sequence

MEPKSLSPYLHLLLILVAVSAIESGSVGTGFSIEEATLKDLRSAFFNQTNLTSRRLVEFYIEQIRKYNASLGGVIEVNPDALDLADAADRKRRASTTPSALPLLHGIPVLVKDNIATKDKLNTTAGSFALLSSVVARDAGVVTKLRKAGAIILGKASLSQWSGFRSSNAPSGWNARSGQGREPYTLGDPCGSSSGSAISVSANMAAVAIGTETDGSLLCPAAYNSVVAIKPTVGLTSRAGVIPISTRQDTVGPIGRTVADAAYVLDAIVGYDAFDQATYCRSQFIPKDGYGQFLKADGLRGKRLGIVEDFSIGIRPSIVQAFQEALQILSKRGAILVSNLKIRDFKTIANPGESAKVTALLNEFRVSVNAYLEGLVSSPVQSLSGVIEFNKQHVHLEEMAKYGQDFLLEANRTNGIGLGAALSKLRALSREGFEKTMTDNRLDAIIAPSEIICSFLAIGGLPGITVPAGYTPTGKPFGFYFGGLRGYEPRLIEIAYDFEQATKTRKPPPLSKLARSSL
Homology
BLAST of Spg001276 vs. NCBI nr
Match: XP_022146229.1 (putative amidase C869.01 [Momordica charantia])

HSP 1 Score: 665.2 bits (1715), Expect = 4.5e-187
Identity = 356/511 (69.67%), Postives = 411/511 (80.43%), Query Frame = 0

Query: 1   MEPKSLSPYLHLLLILVAVSAIESGSV-GTGFSIEEATLKDLRSAFFNQTNLTSRRLVEF 60
           ME   LS    L LILVAVS   S SV   GF IEEA LKDL  A F Q  LTSR+LVEF
Sbjct: 1   METPRLSLSFSLFLILVAVSPFWSYSVRPAGFPIEEAGLKDLHLA-FKQNKLTSRQLVEF 60

Query: 61  YIEQIRKYNASLGGVIEVNPDALDLADAADRKRRASTTPSALPLLHGIPVLVKDNIATKD 120
           YI+QIR+YN  L GVIEVNPDAL LAD ADR+R+A   P +LPLLHGIPVLVKDN+ATKD
Sbjct: 61  YIKQIRRYNPRLRGVIEVNPDALYLADKADRERKAK-APGSLPLLHGIPVLVKDNMATKD 120

Query: 121 KLNTTAGSFALLSSVVARDAGVVTKLRKAGAIILGKASLSQWSGFRSSNAPSGWNARSGQ 180
           KLNTTAGS ALL SVV RDAG V +LRKAGAIILGKAS+S+W+GFRS+ AP+GWNAR+GQ
Sbjct: 121 KLNTTAGSLALLGSVVPRDAGAVMRLRKAGAIILGKASMSEWAGFRSNGAPTGWNARAGQ 180

Query: 181 GREPYTLGDPCGSSSGSAISVSANMAAVAIGTETDGSLLCPAAYNSVVAIKPTVGLTSRA 240
           GREPYTLG PCGSSSGSAISVSANMAAVA+GTETDGS+LCP+++NSVV IKPT+GLTS A
Sbjct: 181 GREPYTLGLPCGSSSGSAISVSANMAAVALGTETDGSILCPSSFNSVVGIKPTLGLTSTA 240

Query: 241 GVIPISTRQDTVGPIGRTVADAAYVLDAIVGYDAFDQATYCRSQFIPKDGYGQFLKADGL 300
           GV+PIS RQDTVGPI RTV+DAAYVLD IVG D  D +TY  S++IP+ GYGQFLKADGL
Sbjct: 241 GVVPISPRQDTVGPICRTVSDAAYVLDVIVGTDPLDNSTYKASRYIPRGGYGQFLKADGL 300

Query: 301 RGKRLGIVEDFSIGIRPSIVQAFQEALQILSKRGAILVSNLKIRDFKTIANPGESAKVTA 360
           RGKRLGIVEDF   + PS++ AF+E   ILSKRGAILV NLKI +   I N  ES +V A
Sbjct: 301 RGKRLGIVEDFFGVVDPSLIPAFEEIFTILSKRGAILVDNLKIDNLGAIYNNTESGEVAA 360

Query: 361 LLNEFRVSVNAYLEGLVSSPVQSLSGVIEFNKQHVHLEEMAKYGQDFLLEANRTNGIGLG 420
           LLNEF+VS+NAYL+ LVSSP++SL+  I FN++H  LE++ +YGQD  L+A  TNG+G+ 
Sbjct: 361 LLNEFKVSLNAYLKELVSSPIRSLAEAIAFNQKHSSLEKIKEYGQDLFLQAEATNGMGIN 420

Query: 421 AALSKLRALSREGFEKTMTDNRLDAIIAPSEIICSFLAIGGLPGITVPAGYTPTGKPFGF 480
             LSKL  LS++G EKTM  N+LDAI+ PS II   LAIGG PGITVPAGY+P+G PFG 
Sbjct: 421 GLLSKLGKLSKDGLEKTMIKNKLDAIMTPSAIISPILAIGGFPGITVPAGYSPSGTPFGI 480

Query: 481 YFGGLRGYEPRLIEIAYDFEQATKTRKPPPL 511
            FGGL+GYEP+LIEIAY FEQATK R+ PPL
Sbjct: 481 DFGGLKGYEPKLIEIAYGFEQATKRRRKPPL 509

BLAST of Spg001276 vs. NCBI nr
Match: XP_008457659.1 (PREDICTED: putative amidase C869.01 isoform X1 [Cucumis melo] >KAA0045712.1 putative amidase isoform X1 [Cucumis melo var. makuwa] >TYJ99568.1 putative amidase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 587.0 bits (1512), Expect = 1.6e-163
Identity = 320/519 (61.66%), Postives = 391/519 (75.34%), Query Frame = 0

Query: 1   MEPKSLSPYLHLLLILVAVSAI---ESGSVGTGFSIEEATLKDLRSAFFNQTNLTSRRLV 60
           M  +S   Y+ +LL L+A+ ++    S S  T FSIEEATLKD + AF+ Q  LTSR+LV
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFY-QNKLTSRQLV 60

Query: 61  EFYIEQIRKYNASLGGVIEVNPDALDLADAADRKRRASTTPSALPLLHGIPVLVKDNIAT 120
           EFY+EQ+R+ N  L G+IEVNPDAL+ A  AD KR+ S+  S  P LHGIPVLVKDNIAT
Sbjct: 61  EFYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSP-LHGIPVLVKDNIAT 120

Query: 121 KDKLNTTAGSFALLSSVVARDAGVVTKLRKAGAIILGKASLSQWSGFRSSNAPSGWNARS 180
           KDKLNTTAGSFALL S+V RDAGVVTKLRKAGAII GKASLS+WSGFRS   P+GW+AR 
Sbjct: 121 KDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARG 180

Query: 181 GQGREPYTLGDPCGSSSGSAISVSANMAAVAIGTETDGSLLCPAAYNSVVAIKPTVGLTS 240
           GQG+ PYT+G+PCGSSSGSAISV+ANM  V++GTETDGS+LCP+  NSVV IKPTVGLTS
Sbjct: 181 GQGKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTS 240

Query: 241 RAGVIPISTRQDTVGPIGRTVADAAYVLDAIVGYDAFDQATYCRSQFIPKDGYGQFLKAD 300
           RAGV+PIS RQDTVGPI RTVADAAYVLDAI G D +D +T   S++IP+ GYGQFL+A+
Sbjct: 241 RAGVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAE 300

Query: 301 GLRGKRLGIVED-FSIGIRPSI-VQAFQEALQILSKRGAILVSNLKIRDFKTIANPGESA 360
           GL+GKR+GIV   +  G      + AF++  + L + GAILV NL I  F  I     S 
Sbjct: 301 GLKGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITG-SSSG 360

Query: 361 KVTALLNEFRVSVNAYLEGLVSSPVQSLSGVIEFNKQHVHLEEMAKYGQDFLLEANRTNG 420
           + TALL EF++S+NAYL+ LV+SP++SLS  IEFNK++  LE++ +YGQ+  LEA  T G
Sbjct: 361 EWTALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKG 420

Query: 421 IGLG--AALSKLRALSREGFEKTMTDNRLDAIIAPSEIICSFLAIGGLPGITVPAGYTPT 480
           IG    AAL++L  LS+EGFE+ M  N+LDAI AP  +I  FLAIGG PG++VPAGY P 
Sbjct: 421 IGGAEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQ 480

Query: 481 GKPFGFYFGGLRGYEPRLIEIAYDFEQATKTRKPPPLSK 513
           G PFG  FGGL+G++PRLIEIAY FE  T  RK P L +
Sbjct: 481 GLPFGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 516

BLAST of Spg001276 vs. NCBI nr
Match: XP_004147024.3 (probable amidase At4g34880 [Cucumis sativus])

HSP 1 Score: 585.5 bits (1508), Expect = 4.5e-163
Identity = 317/517 (61.32%), Postives = 390/517 (75.44%), Query Frame = 0

Query: 1   MEPKSLSPYLHLLLILVAV-SAIESGSVGTGFSIEEATLKDLRSAFFNQTNLTSRRLVEF 60
           M  +S   Y+ +LL L+A+ S+  S S  T FSIEEATLKDL+ AF+ Q  LTS +LVEF
Sbjct: 1   MAAQSFPIYISMLLGLLAILSSYGSCSFDTNFSIEEATLKDLQLAFY-QNKLTSTQLVEF 60

Query: 61  YIEQIRKYNASLGGVIEVNPDALDLADAADRKRRASTTPSALPLLHGIPVLVKDNIATKD 120
           Y+EQ+R++N  L G+IEVNPDAL+ A  AD +R+ S +P +L  LHGIPVLVKDNIATKD
Sbjct: 61  YLEQVRRFNPILNGIIEVNPDALNQASQADLERKRS-SPRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLSSVVARDAGVVTKLRKAGAIILGKASLSQWSGFRSSNAPSGWNARSGQ 180
           +LNTTAGSFALL S+V RDAGVVTKLRKAGAII GKASLS+WS FRS   P+GW+AR GQ
Sbjct: 121 QLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEQPNGWSARGGQ 180

Query: 181 GREPYTLGDPCGSSSGSAISVSANMAAVAIGTETDGSLLCPAAYNSVVAIKPTVGLTSRA 240
           G+ PYT+G+PCGSSSGSAISV+ANM  V++GTETDGS+LCP+  NSVV IKPTVGLTSRA
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVIPISTRQDTVGPIGRTVADAAYVLDAIVGYDAFDQATYCRSQFIPKDGYGQFLKADGL 300
           GV+PIS RQDTVGPI RTVADAAYVLDAI G D +D +T   S+++PK GYGQFLK DGL
Sbjct: 241 GVVPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYVPKGGYGQFLKEDGL 300

Query: 301 RGKRLGIVED-FSIGIRPSI-VQAFQEALQILSKRGAILVSNLKIRDFKTIANPGESAKV 360
           +GKR+GIV   +  G      + AF++  + L + GAILV NL I  F  I     S + 
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINSFHVITG-SSSGEW 360

Query: 361 TALLNEFRVSVNAYLEGLVSSPVQSLSGVIEFNKQHVHLEEMAKYGQDFLLEANRTNGIG 420
           TA+L EF++S+N YL+ LV+SP++SLS  IEFN+++  LE++ +YGQ+  LEA  T GIG
Sbjct: 361 TAVLAEFKISINVYLKQLVASPIRSLSDAIEFNRKNSKLEKLKEYGQELFLEAEATKGIG 420

Query: 421 LG--AALSKLRALSREGFEKTMTDNRLDAIIAPSEIICSFLAIGGLPGITVPAGYTPTGK 480
               AAL++L  LS+EGFE+ M  N+LDAI AP  +I  FLAIGG PG++VPAGY P G 
Sbjct: 421 GAEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISHFLAIGGFPGVSVPAGYNPQGL 480

Query: 481 PFGFYFGGLRGYEPRLIEIAYDFEQATKTRKPPPLSK 513
           PFG  FGGL+G+EPRLIEIAY FE  T  RK P L +
Sbjct: 481 PFGIGFGGLKGFEPRLIEIAYGFEHLTMGRKSPSLGR 514

BLAST of Spg001276 vs. NCBI nr
Match: ADN34007.1 (amidase [Cucumis melo subsp. melo])

HSP 1 Score: 583.6 bits (1503), Expect = 1.7e-162
Identity = 318/507 (62.72%), Postives = 384/507 (75.74%), Query Frame = 0

Query: 12  LLLILVAVSAIESGSVG--TGFSIEEATLKDLRSAFFNQTNLTSRRLVEFYIEQIRKYNA 71
           LL +L  +S   SGS    T FSIEEATLKD + AF+ Q  LTSR+LVEFY+EQ+R+ N 
Sbjct: 2   LLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFY-QNKLTSRQLVEFYLEQVRRLNP 61

Query: 72  SLGGVIEVNPDALDLADAADRKRRASTTPSALPLLHGIPVLVKDNIATKDKLNTTAGSFA 131
            L G+IEVNPDAL+ A  AD KR+ S+  S  P LHGIPVLVKDNIATKDKLNTTAGSFA
Sbjct: 62  ILKGIIEVNPDALNQASQADLKRKRSSLRSLSP-LHGIPVLVKDNIATKDKLNTTAGSFA 121

Query: 132 LLSSVVARDAGVVTKLRKAGAIILGKASLSQWSGFRSSNAPSGWNARSGQGREPYTLGDP 191
           LL S+V RDAGVVTKLRKAGAII GKASLS+WSGFRS   P+GW+AR GQG+ PYT+G+P
Sbjct: 122 LLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQGKNPYTMGEP 181

Query: 192 CGSSSGSAISVSANMAAVAIGTETDGSLLCPAAYNSVVAIKPTVGLTSRAGVIPISTRQD 251
           CGSSSGSAISV+ANM  V++GTETDGS+LCP+  NSVV IKPTVGLTSRAGV+PIS RQD
Sbjct: 182 CGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQD 241

Query: 252 TVGPIGRTVADAAYVLDAIVGYDAFDQATYCRSQFIPKDGYGQFLKADGLRGKRLGIVED 311
           TVGPI RTVADAAYVLDAI G D +D +T   S++IP+ GYGQFL+A+GL+GKR+GIV  
Sbjct: 242 TVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGLKGKRIGIVRK 301

Query: 312 -FSIGIRPSI-VQAFQEALQILSKRGAILVSNLKIRDFKTIANPGESAKVTALLNEFRVS 371
            +  G      + AF++  + L + GAILV NL I  F  I     S + TALL EF++S
Sbjct: 302 LYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITG-SSSGEWTALLAEFKIS 361

Query: 372 VNAYLEGLVSSPVQSLSGVIEFNKQHVHLEEMAKYGQDFLLEANRTNGIGLG--AALSKL 431
           +NAYL+ LV+SP++SLS  IEFNK++  LE++ +YGQ+  LEA  T GIG    AAL++L
Sbjct: 362 LNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGGAEKAALARL 421

Query: 432 RALSREGFEKTMTDNRLDAIIAPSEIICSFLAIGGLPGITVPAGYTPTGKPFGFYFGGLR 491
             LS+EGFE+ M  N+LDAI AP  +I  FLAIGG PG++VPAGY P G PFG  FGGL+
Sbjct: 422 AKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLPFGIGFGGLK 481

Query: 492 GYEPRLIEIAYDFEQATKTRKPPPLSK 513
           G++PRLIEIAY FE  T  RK P L +
Sbjct: 482 GFDPRLIEIAYGFEHLTMGRKSPSLGR 505

BLAST of Spg001276 vs. NCBI nr
Match: XP_008457661.1 (PREDICTED: putative amidase C869.01 [Cucumis melo] >ADN34008.1 amidase [Cucumis melo subsp. melo] >KAA0045711.1 putative amidase [Cucumis melo var. makuwa] >TYJ99569.1 putative amidase [Cucumis melo var. makuwa])

HSP 1 Score: 580.5 bits (1495), Expect = 1.5e-161
Identity = 320/518 (61.78%), Postives = 397/518 (76.64%), Query Frame = 0

Query: 1   MEPKSLSPYLHLLLILVAV-SAIESGSVGTGFSIEEATLKDLRSAFFNQTNLTSRRLVEF 60
           M  +S   Y+ +LL L+A+ S+  S S  T  SIEEATL DL+ AF+ Q  LTSR+LVEF
Sbjct: 1   MAGQSFLLYVSMLLGLMAILSSYGSCSFHTKLSIEEATLIDLQHAFY-QNKLTSRQLVEF 60

Query: 61  YIEQIRKYNASLGGVIEVNPDALDLADAADRKRRASTTPSALPLLHGIPVLVKDNIATKD 120
           Y+EQ+R++N  L G+IEVNPDALD A  AD +R+ S +P +L  LHGIPVLVKDNIATKD
Sbjct: 61  YLEQVRRFNPILKGIIEVNPDALDQASRADIERKKS-SPRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLSSVVARDAGVVTKLRKAGAIILGKASLSQWSGFRSSNAPSGWNARSGQ 180
           KLNTTAGSFALL S+V RDAGVVTKLR AGAII GKASLS+WS FRS+  PSGW+AR GQ
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRMAGAIIFGKASLSEWSYFRSNELPSGWSARGGQ 180

Query: 181 GREPYTLGDPCGSSSGSAISVSANMAAVAIGTETDGSLLCPAAYNSVVAIKPTVGLTSRA 240
           G+ PY LG+PCGSSSGSAISV+ANM  V++GTETDGS+LCP+  NSVV IKPTVGLTSRA
Sbjct: 181 GKNPYILGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVIPISTRQDTVGPIGRTVADAAYVLDAIVGYDAFDQATYCRSQFIPKDGYGQFLKADGL 300
           GVIPIS+RQD+VGPI RTV+DA YVLDAIVG D +D +T   S++IPK GYGQFL+A GL
Sbjct: 241 GVIPISSRQDSVGPICRTVSDATYVLDAIVGADRYDNSTIEASKYIPKGGYGQFLRAGGL 300

Query: 301 RGKRLGIVEDF-SIGIRPSIV-QAFQEALQILSKRGAILVSNLKIRDFKTIANPGESAKV 360
           +GKR+GIV +F   G   +   QA+++ L+ L K GAILV N  I + + I + G S + 
Sbjct: 301 KGKRIGIVREFYDFGHDETFYPQAYEKVLKTLKKGGAILVDNRTIDNLQLIFD-GSSGEQ 360

Query: 361 TALLNEFRVSVNAYLEGLVSSPVQSLSGVIEFNKQHVHLEEMAKYGQDFLLEANRTNGIG 420
            ALL EF++S+NAYL+ LV+SP++SLS  IEFNK++  LE + +YGQ+  L+A  TNGIG
Sbjct: 361 IALLAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLENL-EYGQELFLKAEATNGIG 420

Query: 421 LG--AALSKLRALSREGFEKTMTDNRLDAIIAPSEIICSFLAIGGLPGITVPAGYTPTGK 480
               AAL++L  LS++GFE+ M  N+LDAI AP  +I S  AIGG PG++VPAGY P G 
Sbjct: 421 DAEKAALARLAKLSKDGFERLMIKNKLDAIAAPGSLISSVFAIGGFPGVSVPAGYDPQGV 480

Query: 481 PFGFYFGGLRGYEPRLIEIAYDFEQATKTRKPPPLSKL 514
           P+G  FGGL+G+EPRLIEIAY FE  TK+RKPP ++ L
Sbjct: 481 PYGITFGGLKGFEPRLIEIAYGFEHLTKSRKPPSINGL 514

BLAST of Spg001276 vs. ExPASy Swiss-Prot
Match: A0A1P8B760 (Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=1)

HSP 1 Score: 501.9 bits (1291), Expect = 8.6e-141
Identity = 281/507 (55.42%), Postives = 363/507 (71.60%), Query Frame = 0

Query: 13  LLILVAVSAIESGSVGTG--------FSIEEATLKDLRSAFFNQTNLTSRRLVEFYIEQI 72
           LLIL+ +S     SVG+         FSI+EAT++D+R A FN+  LTS++LVE Y+E I
Sbjct: 12  LLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVA-FNEKRLTSKQLVELYLEAI 71

Query: 73  RKYNASLGGVIEVNPDALDLADAADRKRRASTTPSALPLLHGIPVLVKDNIATKDKLNTT 132
            K N  L  VIE NPDAL  A+ ADR+R    T + LP+LHG+PVL+KD+I+TKDKLNTT
Sbjct: 72  SKLNPILHAVIETNPDALIQAEIADRERDLKNT-TKLPILHGVPVLLKDSISTKDKLNTT 131

Query: 133 AGSFALLSSVVARDAGVVTKLRKAGAIILGKASLSQWSGFRSSNAPSGWNARSGQGREPY 192
           AGSFALL SVVARDAGVV +LR++GA+ILGKASLS+W+ FRS + P GW+AR  QG+ PY
Sbjct: 132 AGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSARGLQGKNPY 191

Query: 193 TL-GDPCGSSSGSAISVSANMAAVAIGTETDGSLLCPAAYNSVVAIKPTVGLTSRAGVIP 252
            L  +P GSSSGSAISV+AN+ AV++GTETDGS+L PA+ NSVV IKP+VGLTSRAGV+P
Sbjct: 192 VLSANPSGSSSGSAISVTANLVAVSLGTETDGSILSPASQNSVVGIKPSVGLTSRAGVVP 251

Query: 253 ISTRQDTVGPIGRTVADAAYVLDAIVGYDAFDQATYCRSQFIPKDGYGQFLKADGLRGKR 312
           IS RQD++GPI RTV+DA ++LDAIVGYD  D+AT   S+FIP+ GY QFL   GL+GKR
Sbjct: 252 ISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLTTSGLKGKR 311

Query: 313 LGIVEDFSIGIRPSIVQAFQEALQILSKRGAILVSNLKIRDFKTIANPGESAKVTALLNE 372
           LGIV   S              ++ L + GAI+++NL I + + I    +S +  ALL E
Sbjct: 312 LGIVMKHS--------SLLDHHIKTLRREGAIVINNLTIPNIEVIVGGTDSGEEIALLAE 371

Query: 373 FRVSVNAYLEGLVSSPVQSLSGVIEFNKQHVHLEEMAKYGQDFLLEANRTNGIG--LGAA 432
           F++S+NAYL+ LV SPV+SL+ VI +N++    E++ ++GQ+  L A  T+G+G     A
Sbjct: 372 FKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKEKTA 431

Query: 433 LSKLRALSREGFEKTMTDNRLDAIIAPSEIICSFLAIGGLPGITVPAGYTPTGKPFGFYF 492
           L K++ LSR G EK + +N+LDAI+     + S LAIGG PGI VPAGY   G P+G  F
Sbjct: 432 LQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYGISF 491

Query: 493 GGLRGYEPRLIEIAYDFEQATKTRKPP 509
           GGLR  EP+LIEIA+ FEQAT  RKPP
Sbjct: 492 GGLRFSEPKLIEIAFAFEQATLIRKPP 508

BLAST of Spg001276 vs. ExPASy Swiss-Prot
Match: Q9URY4 (Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.01 PE=3 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.2e-70
Identity = 185/510 (36.27%), Postives = 278/510 (54.51%), Query Frame = 0

Query: 32  SIEEATLKDLRSAFFNQTNLTSRRLVEFYIEQIRKYNASLGGVIEVNPDALDLADAADRK 91
           ++E+AT+  L++ +     LTS  +V  Y+++  + N  + G++++NPD L +A   D +
Sbjct: 65  TLEDATIDQLQN-YMENGILTSTDIVHCYLDRYLQVNPYVNGILQLNPDVLTIASELDDE 124

Query: 92  RRASTTPSALPLLHGIPVLVKDNIATKDKLNTTAGSFALLSSVVARDAGVVTKLRKAGAI 151
           R           LHGIP +VKDN ATKDK++TTAGS+ALL S+V RDA VV +LR+AGA+
Sbjct: 125 RANGIIRGP---LHGIPFIVKDNFATKDKMDTTAGSYALLGSIVPRDAYVVKQLREAGAV 184

Query: 152 ILGKASLSQWSGFRSSNAPSGWNARSGQGREPYTLG-DPCGSSSGSAISVSANMAAVAIG 211
           + G A+LS+W+  RS++   G++AR GQ R P+ L  +P GSSSGSAISV++NM A A+G
Sbjct: 185 LFGHATLSEWADMRSNDYSEGYSARGGQSRCPFNLTVNPGGSSSGSAISVASNMIAFALG 244

Query: 212 TETDGSLLCPAAYNSVVAIKPTVGLTSRAGVIPISTRQDTVGPIGRTVADAAYVLDAIVG 271
           TETDGS++ PA  N VV +KPTVGLTSR GVIP S  QDT GPI RTV DA YV  ++ G
Sbjct: 245 TETDGSIIDPAMRNGVVGLKPTVGLTSRYGVIPESEHQDTTGPIARTVRDAVYVFQSMWG 304

Query: 272 YDAFDQATYCRSQFIPKDG-YGQFL-KADGLRGKRLGIV-EDFSIGIRPSIVQAFQEALQ 331
            D  D  T  ++   P+DG Y +FL     L G R G+  +      +   +    E ++
Sbjct: 305 IDENDIYTLNQTGKTPEDGDYMKFLSNKTSLEGARFGLPWKRLWQNAKADEIDRLLEVVK 364

Query: 332 ILSKRGAILVSNLKIRDFKTIANPG--------ESAKVTALLNEFRVSVNAYLEGLVSSP 391
            + + GAI+ +N    +   I+N G          ++ T +  +F  ++ +YL  + ++ 
Sbjct: 365 QIEEAGAIVYNNTNFYNLDVISNDGWNWELGSVNESEYTVVKVDFYNNIKSYLSEVKNTE 424

Query: 392 VQSLSGVIEFNKQHVHLE-------EMAKYGQDFLLEANRTNGI---GLGAALSKLRALS 451
           + SL  ++E+N +++  E            GQD  L +    G+       A+  +R  S
Sbjct: 425 IHSLEDIVEYNNKYMGTEGGKPNIVPAFSSGQDGFLASLEWGGVKNETYWQAVEYVRRTS 484

Query: 452 R-EGFE--------KTMTDNRLDAIIAPS--EIICSFLAIGGLPGITVPAGYTPTGKPFG 509
           + EG +        KT     L+ ++ PS   I     A  G P IT+P G    G+PFG
Sbjct: 485 QDEGIDYALNYTDPKTNDSFILNGLLVPSGTSITYQQAAKAGYPMITLPIGVKTNGRPFG 544

BLAST of Spg001276 vs. ExPASy Swiss-Prot
Match: D4B3C8 (Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02965 PE=1 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 2.7e-65
Identity = 173/491 (35.23%), Postives = 259/491 (52.75%), Query Frame = 0

Query: 46  FNQTNLTSRRLVEFYIEQIRKYNASLGGVIEVNPDALDLADAADRKRRASTTPSALPLLH 105
           + Q N+    +V+ Y+ +I + N+++  V E+NPDAL +A   D +R+          LH
Sbjct: 98  YRQGNVLIIVVVQTYVARIAEVNSTVRAVTEINPDALTIAKQMDNERKMGKLRGP---LH 157

Query: 106 GIPVLVKDNIATKDKLNTTAGSFALLSSVVARDAGVVTKLRKAGAIILGKASLSQWSGFR 165
           G+P+++K+NI T DK+++TAGS+A+  +  + DA V TKLR+AG +I+GK+  SQW+ FR
Sbjct: 158 GLPIVIKNNIFTDDKMSSTAGSYAIFGARTSADATVATKLREAGLVIMGKSGASQWANFR 217

Query: 166 SSNAPSGWNARSGQGREPYTLG-DPCGSSSGSAISVSANMAAVAIGTETDGSLLCPAAYN 225
           S N+ +GW+A  GQ    Y    DP GSSSGS ++    +A   +GTET GS++ PA  +
Sbjct: 218 SLNSTNGWSAYGGQVTAAYIKNQDPSGSSSGSGVASDLGLAFATLGTETSGSIVSPADKS 277

Query: 226 SVVAIKPTVGLTSRAGVIPISTRQDTVGPIGRTVADAAYVLDAIVGYDAFDQATYCRSQF 285
           ++V +KPTVGLTSR  V+PIS RQDTVGP+ R+V DAAY+L  I G D+ D  T      
Sbjct: 278 NIVGLKPTVGLTSRRFVVPISERQDTVGPMARSVKDAAYLLQVIAGKDSNDNYT----SA 337

Query: 286 IPKDGYGQFLKA---DGLRGKRLGIVEDF--SIGIRPSIVQAFQEALQILSKRGAILVSN 345
           IP D    ++KA   + L+GKR+G+  +     G   ++V  F +AL ++ K GAI+V N
Sbjct: 338 IPFDTIPDYVKACDINALKGKRIGVPRNVIKIFGSPQTVVDQFNQALAVMKKAGAIIVEN 397

Query: 346 LKIRDFKTIAN---PGESAKVTALLNEFRVSVNAYLEGLVSSP--VQSLSGVIEFNKQHV 405
                F   A    P +     +L N     + A+ + L  +P  +  L  +  F  QH 
Sbjct: 398 TDFTSFAEFAQSPIPDDILYADSLTN-----LPAFFKQLKVNPHNITDLESLRRFT-QHH 457

Query: 406 HLEE-----MAKYGQDFLLEANRTNGIGLGAALSKLRALSREGFEKTMTDNRLDAIIAPS 465
            LEE      A++          T+          ++  +  G    +  ++LDA + P+
Sbjct: 458 RLEEYPSRDTARWDIALQKGIKNTDPKFWPMYQKNVKFGNEGGILGALRRHKLDAAVLPT 517

Query: 466 EIICSFLAIGGLPGITVPAGYTPTGK---------------PFGFYFGGLRGYEPRLIEI 506
           ++     A+ G P ITVP G  P G                P G  F G    E +LI +
Sbjct: 518 DLSPYIPALIGSPIITVPMGVYPNGTKVNHDRELVTSGPGIPIGIGFMGDLWSEEKLIGL 575

BLAST of Spg001276 vs. ExPASy Swiss-Prot
Match: B0JSX3 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=gatA PE=3 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 7.3e-39
Identity = 147/504 (29.17%), Postives = 238/504 (47.22%), Query Frame = 0

Query: 37  TLKDLRSAFFNQTNLTSRRLVEFYIEQIRKYNASLGGVIEVNPD-ALDLADAADRKRRAS 96
           +++ L     N+   T+  +   ++ +I+     +   + + PD AL  A   D K    
Sbjct: 3   SIRQLHQQLVNKEK-TAVEIATEFLARIQALEPQVKSFLHLTPDLALAQAQKVDEK---I 62

Query: 97  TTPSALPLLHGIPVLVKDNIATKDKLNTTAGSFALLSSVVARDAGVVTKLRKAGAIILGK 156
               +L LL GIP+ +KDN+ TK  + TT  S  L + V   ++ V  KLR  GA+I+GK
Sbjct: 63  ARGESLHLLAGIPIALKDNLCTKG-IPTTCASRILENFVPPYESTVTQKLRDLGAVIVGK 122

Query: 157 ASLSQWSGFRSSNAPSGWNARSGQGREPYTLGD-PCGSSSGSAISVSANMAAVAIGTETD 216
            +L +++   SS   SG++  +     P+ L   P GSS GSA +V+A    VA+G++T 
Sbjct: 123 TNLDEFA-MGSSTENSGYHVTA----NPWDLSRVPGGSSGGSAAAVAAQECVVALGSDTG 182

Query: 217 GSLLCPAAYNSVVAIKPTVGLTSRAGVIPISTRQDTVGPIGRTVADAAYVLDAIVGYDAF 276
           GS+  PA++  VV +KPT GL SR G++  ++  D +GP GRTV DAA +L AI GYD  
Sbjct: 183 GSIRQPASFCGVVGLKPTYGLVSRFGLVAYASSLDQIGPFGRTVEDAAILLQAIAGYDPQ 242

Query: 277 DQATYCRSQFIPKDGYGQFLKADGLRGKRLGIV-EDFSIGIRPSIVQAFQEALQILSKRG 336
           D      S  +P   Y QFLK   L+G ++G++ E F  G+   + +A  +AL  L   G
Sbjct: 243 DST----SLNLPIPDYSQFLKT-SLKGLKIGVIKETFGEGLDQVVAEAVNQALAQLKALG 302

Query: 337 AILVSNLKIRDFK------TIANPGESAKVTALLNEFRVSVNAYLEGLVSSPVQS-LSGV 396
           A  +  +    F+       I  P E++   A  +  +  +    + L+    ++   G 
Sbjct: 303 A-TIKEISCPRFRYGLPTYYIIAPSEASANLARYDGVKYGIREDADSLIDMYTKTRAKGF 362

Query: 397 IEFNKQHVHLEEMAKYGQDFLLEANRTNGIGLGAALSKLRALSREGFEKTMTDNRLDAII 456
               K+ + L         + L A   +   L A   K+R L +E F++      +D ++
Sbjct: 363 GAEVKRRIML-------GTYTLSAGYYDAYYLKA--QKVRTLIKEDFDRAF--QSVDVLV 422

Query: 457 APSEIICSFLA---------------------IGGLPGITVPAGYTPTGKPFGFYFGGLR 510
           +P+    +F A                     + GLPG+++P G+   G P G    G  
Sbjct: 423 SPTSPTTAFKAGEKTADPLSMYLSDLMTIPVNLAGLPGLSLPCGFDGQGLPIGLQLVGNV 479

BLAST of Spg001276 vs. ExPASy Swiss-Prot
Match: B0KBN4 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=gatA PE=3 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 3.6e-38
Identity = 146/507 (28.80%), Postives = 240/507 (47.34%), Query Frame = 0

Query: 37  TLKDLRSAFFNQTNLTSRRLVEFYIEQIRKYNASLGGVIEVNPD-ALDLADAADRKRRAS 96
           T+ +LR     +  +++  + + Y+E+I++    +  +I +  D AL  A  AD K +  
Sbjct: 7   TIHELRE-LLKKREVSALEVTKSYLERIKEVEPKIDALITITEDFALQRAKEADEKIKNG 66

Query: 97  TTPSALPLLHGIPVLVKDNIATKDKLNTTAGSFALLSSVVARDAGVVTKLRKAGAIILGK 156
              +    L GIPV++KDNI+T + + TT  S  L + +   +A VV KL + G IILGK
Sbjct: 67  EDTA----LTGIPVIIKDNIST-EGIKTTCSSKMLENYIPPYNATVVEKLLEEGVIILGK 126

Query: 157 ASLSQWSGFRSSNAPSGWNARSGQGREPYTLGD-PCGSSSGSAISVSANMAAVAIGTETD 216
           ++L +++   S+      N+     + P+ L   P GSS GSA +++A+ AA A+G++T 
Sbjct: 127 SNLDEFAMGSSTE-----NSAFKTTKNPWDLSRVPGGSSGGSAAAIAADEAAFALGSDTG 186

Query: 217 GSLLCPAAYNSVVAIKPTVGLTSRAGVIPISTRQDTVGPIGRTVADAAYVLDAIVGYDAF 276
           GS+  PA+   VV +KPT GL SR G++  ++  D +GP  + V D A VL+ I+G+D  
Sbjct: 187 GSIRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIGHDPK 246

Query: 277 DQATYCRSQFIPKDGYGQFLKADGLRGKRLGIVED-FSIGIRPSIVQAFQEALQILSKRG 336
           D      S  I K  Y  +LK D ++G R+G+ ++ F  GI   + +  QE++++L   G
Sbjct: 247 DST----SLKIDKPDYTSYLKED-IKGLRIGVAKEFFGEGIEEGVKETVQESIKVLQDLG 306

Query: 337 AILVSNLKIRDFKTIANPGESAKVTALLNEFRVSVNAYLEGLVSSPVQSLSGVIEFNKQH 396
           A ++ ++ I  +   A P      +A  +      +    G ++   + L  +       
Sbjct: 307 AEII-DISI-PYVEYALPAYYIIASAEASSNLARYDGIRYGHIAGKYEDLIDMY------ 366

Query: 397 VHLEEMAKYGQDFLLEANRTNGIGLGAALS-----------KLRALSREGFEKTMTDNRL 456
                M    + F  E  R   +G  A  S           K+R L +  FEK     + 
Sbjct: 367 -----MVTRSEGFGKEVKRRIMLGTYALSSGYYDAYYKKALKVRTLIKNDFEKAF--EKC 426

Query: 457 DAIIAPSEIICSF---------------------LAIGGLPGITVPAGYTPTGKPFGFYF 509
           D II P+    +F                     + I GLPGI++P G +  G P G   
Sbjct: 427 DVIIGPTSPTVAFKIGERANDPLAMYLADIYTVSVNIAGLPGISIPCGLS-DGLPVGLQI 481

BLAST of Spg001276 vs. ExPASy TrEMBL
Match: A0A6J1CXI8 (putative amidase C869.01 OS=Momordica charantia OX=3673 GN=LOC111015494 PE=4 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 2.2e-187
Identity = 356/511 (69.67%), Postives = 411/511 (80.43%), Query Frame = 0

Query: 1   MEPKSLSPYLHLLLILVAVSAIESGSV-GTGFSIEEATLKDLRSAFFNQTNLTSRRLVEF 60
           ME   LS    L LILVAVS   S SV   GF IEEA LKDL  A F Q  LTSR+LVEF
Sbjct: 1   METPRLSLSFSLFLILVAVSPFWSYSVRPAGFPIEEAGLKDLHLA-FKQNKLTSRQLVEF 60

Query: 61  YIEQIRKYNASLGGVIEVNPDALDLADAADRKRRASTTPSALPLLHGIPVLVKDNIATKD 120
           YI+QIR+YN  L GVIEVNPDAL LAD ADR+R+A   P +LPLLHGIPVLVKDN+ATKD
Sbjct: 61  YIKQIRRYNPRLRGVIEVNPDALYLADKADRERKAK-APGSLPLLHGIPVLVKDNMATKD 120

Query: 121 KLNTTAGSFALLSSVVARDAGVVTKLRKAGAIILGKASLSQWSGFRSSNAPSGWNARSGQ 180
           KLNTTAGS ALL SVV RDAG V +LRKAGAIILGKAS+S+W+GFRS+ AP+GWNAR+GQ
Sbjct: 121 KLNTTAGSLALLGSVVPRDAGAVMRLRKAGAIILGKASMSEWAGFRSNGAPTGWNARAGQ 180

Query: 181 GREPYTLGDPCGSSSGSAISVSANMAAVAIGTETDGSLLCPAAYNSVVAIKPTVGLTSRA 240
           GREPYTLG PCGSSSGSAISVSANMAAVA+GTETDGS+LCP+++NSVV IKPT+GLTS A
Sbjct: 181 GREPYTLGLPCGSSSGSAISVSANMAAVALGTETDGSILCPSSFNSVVGIKPTLGLTSTA 240

Query: 241 GVIPISTRQDTVGPIGRTVADAAYVLDAIVGYDAFDQATYCRSQFIPKDGYGQFLKADGL 300
           GV+PIS RQDTVGPI RTV+DAAYVLD IVG D  D +TY  S++IP+ GYGQFLKADGL
Sbjct: 241 GVVPISPRQDTVGPICRTVSDAAYVLDVIVGTDPLDNSTYKASRYIPRGGYGQFLKADGL 300

Query: 301 RGKRLGIVEDFSIGIRPSIVQAFQEALQILSKRGAILVSNLKIRDFKTIANPGESAKVTA 360
           RGKRLGIVEDF   + PS++ AF+E   ILSKRGAILV NLKI +   I N  ES +V A
Sbjct: 301 RGKRLGIVEDFFGVVDPSLIPAFEEIFTILSKRGAILVDNLKIDNLGAIYNNTESGEVAA 360

Query: 361 LLNEFRVSVNAYLEGLVSSPVQSLSGVIEFNKQHVHLEEMAKYGQDFLLEANRTNGIGLG 420
           LLNEF+VS+NAYL+ LVSSP++SL+  I FN++H  LE++ +YGQD  L+A  TNG+G+ 
Sbjct: 361 LLNEFKVSLNAYLKELVSSPIRSLAEAIAFNQKHSSLEKIKEYGQDLFLQAEATNGMGIN 420

Query: 421 AALSKLRALSREGFEKTMTDNRLDAIIAPSEIICSFLAIGGLPGITVPAGYTPTGKPFGF 480
             LSKL  LS++G EKTM  N+LDAI+ PS II   LAIGG PGITVPAGY+P+G PFG 
Sbjct: 421 GLLSKLGKLSKDGLEKTMIKNKLDAIMTPSAIISPILAIGGFPGITVPAGYSPSGTPFGI 480

Query: 481 YFGGLRGYEPRLIEIAYDFEQATKTRKPPPL 511
            FGGL+GYEP+LIEIAY FEQATK R+ PPL
Sbjct: 481 DFGGLKGYEPKLIEIAYGFEQATKRRRKPPL 509

BLAST of Spg001276 vs. ExPASy TrEMBL
Match: A0A5D3BN38 (Putative amidase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001380 PE=4 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 7.5e-164
Identity = 320/519 (61.66%), Postives = 391/519 (75.34%), Query Frame = 0

Query: 1   MEPKSLSPYLHLLLILVAVSAI---ESGSVGTGFSIEEATLKDLRSAFFNQTNLTSRRLV 60
           M  +S   Y+ +LL L+A+ ++    S S  T FSIEEATLKD + AF+ Q  LTSR+LV
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFY-QNKLTSRQLV 60

Query: 61  EFYIEQIRKYNASLGGVIEVNPDALDLADAADRKRRASTTPSALPLLHGIPVLVKDNIAT 120
           EFY+EQ+R+ N  L G+IEVNPDAL+ A  AD KR+ S+  S  P LHGIPVLVKDNIAT
Sbjct: 61  EFYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSP-LHGIPVLVKDNIAT 120

Query: 121 KDKLNTTAGSFALLSSVVARDAGVVTKLRKAGAIILGKASLSQWSGFRSSNAPSGWNARS 180
           KDKLNTTAGSFALL S+V RDAGVVTKLRKAGAII GKASLS+WSGFRS   P+GW+AR 
Sbjct: 121 KDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARG 180

Query: 181 GQGREPYTLGDPCGSSSGSAISVSANMAAVAIGTETDGSLLCPAAYNSVVAIKPTVGLTS 240
           GQG+ PYT+G+PCGSSSGSAISV+ANM  V++GTETDGS+LCP+  NSVV IKPTVGLTS
Sbjct: 181 GQGKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTS 240

Query: 241 RAGVIPISTRQDTVGPIGRTVADAAYVLDAIVGYDAFDQATYCRSQFIPKDGYGQFLKAD 300
           RAGV+PIS RQDTVGPI RTVADAAYVLDAI G D +D +T   S++IP+ GYGQFL+A+
Sbjct: 241 RAGVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAE 300

Query: 301 GLRGKRLGIVED-FSIGIRPSI-VQAFQEALQILSKRGAILVSNLKIRDFKTIANPGESA 360
           GL+GKR+GIV   +  G      + AF++  + L + GAILV NL I  F  I     S 
Sbjct: 301 GLKGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITG-SSSG 360

Query: 361 KVTALLNEFRVSVNAYLEGLVSSPVQSLSGVIEFNKQHVHLEEMAKYGQDFLLEANRTNG 420
           + TALL EF++S+NAYL+ LV+SP++SLS  IEFNK++  LE++ +YGQ+  LEA  T G
Sbjct: 361 EWTALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKG 420

Query: 421 IGLG--AALSKLRALSREGFEKTMTDNRLDAIIAPSEIICSFLAIGGLPGITVPAGYTPT 480
           IG    AAL++L  LS+EGFE+ M  N+LDAI AP  +I  FLAIGG PG++VPAGY P 
Sbjct: 421 IGGAEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQ 480

Query: 481 GKPFGFYFGGLRGYEPRLIEIAYDFEQATKTRKPPPLSK 513
           G PFG  FGGL+G++PRLIEIAY FE  T  RK P L +
Sbjct: 481 GLPFGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 516

BLAST of Spg001276 vs. ExPASy TrEMBL
Match: A0A1S3C7A8 (putative amidase C869.01 isoform X1 OS=Cucumis melo OX=3656 GN=LOC107990287 PE=4 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 7.5e-164
Identity = 320/519 (61.66%), Postives = 391/519 (75.34%), Query Frame = 0

Query: 1   MEPKSLSPYLHLLLILVAVSAI---ESGSVGTGFSIEEATLKDLRSAFFNQTNLTSRRLV 60
           M  +S   Y+ +LL L+A+ ++    S S  T FSIEEATLKD + AF+ Q  LTSR+LV
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFY-QNKLTSRQLV 60

Query: 61  EFYIEQIRKYNASLGGVIEVNPDALDLADAADRKRRASTTPSALPLLHGIPVLVKDNIAT 120
           EFY+EQ+R+ N  L G+IEVNPDAL+ A  AD KR+ S+  S  P LHGIPVLVKDNIAT
Sbjct: 61  EFYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSP-LHGIPVLVKDNIAT 120

Query: 121 KDKLNTTAGSFALLSSVVARDAGVVTKLRKAGAIILGKASLSQWSGFRSSNAPSGWNARS 180
           KDKLNTTAGSFALL S+V RDAGVVTKLRKAGAII GKASLS+WSGFRS   P+GW+AR 
Sbjct: 121 KDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARG 180

Query: 181 GQGREPYTLGDPCGSSSGSAISVSANMAAVAIGTETDGSLLCPAAYNSVVAIKPTVGLTS 240
           GQG+ PYT+G+PCGSSSGSAISV+ANM  V++GTETDGS+LCP+  NSVV IKPTVGLTS
Sbjct: 181 GQGKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTS 240

Query: 241 RAGVIPISTRQDTVGPIGRTVADAAYVLDAIVGYDAFDQATYCRSQFIPKDGYGQFLKAD 300
           RAGV+PIS RQDTVGPI RTVADAAYVLDAI G D +D +T   S++IP+ GYGQFL+A+
Sbjct: 241 RAGVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAE 300

Query: 301 GLRGKRLGIVED-FSIGIRPSI-VQAFQEALQILSKRGAILVSNLKIRDFKTIANPGESA 360
           GL+GKR+GIV   +  G      + AF++  + L + GAILV NL I  F  I     S 
Sbjct: 301 GLKGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITG-SSSG 360

Query: 361 KVTALLNEFRVSVNAYLEGLVSSPVQSLSGVIEFNKQHVHLEEMAKYGQDFLLEANRTNG 420
           + TALL EF++S+NAYL+ LV+SP++SLS  IEFNK++  LE++ +YGQ+  LEA  T G
Sbjct: 361 EWTALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKG 420

Query: 421 IGLG--AALSKLRALSREGFEKTMTDNRLDAIIAPSEIICSFLAIGGLPGITVPAGYTPT 480
           IG    AAL++L  LS+EGFE+ M  N+LDAI AP  +I  FLAIGG PG++VPAGY P 
Sbjct: 421 IGGAEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQ 480

Query: 481 GKPFGFYFGGLRGYEPRLIEIAYDFEQATKTRKPPPLSK 513
           G PFG  FGGL+G++PRLIEIAY FE  T  RK P L +
Sbjct: 481 GLPFGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 516

BLAST of Spg001276 vs. ExPASy TrEMBL
Match: A0A0A0LLK0 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264040 PE=4 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 6.4e-163
Identity = 316/517 (61.12%), Postives = 389/517 (75.24%), Query Frame = 0

Query: 1   MEPKSLSPYLHLLLILVAV-SAIESGSVGTGFSIEEATLKDLRSAFFNQTNLTSRRLVEF 60
           M  +S   Y+ +LL L+A+ S+  S S  T FSIEEATLKDL+ AF+ Q  LTS +LVEF
Sbjct: 1   MAAQSFPIYISMLLGLLAILSSYGSCSFDTNFSIEEATLKDLQLAFY-QNKLTSTQLVEF 60

Query: 61  YIEQIRKYNASLGGVIEVNPDALDLADAADRKRRASTTPSALPLLHGIPVLVKDNIATKD 120
           Y+EQ+R++N  L G+IEVNPDAL+ A  AD +R+ S +P +L  LHGIPV VKDNIATKD
Sbjct: 61  YLEQVRRFNPILNGIIEVNPDALNQASQADLERKRS-SPRSLSPLHGIPVFVKDNIATKD 120

Query: 121 KLNTTAGSFALLSSVVARDAGVVTKLRKAGAIILGKASLSQWSGFRSSNAPSGWNARSGQ 180
           +LNTTAGSFALL S+V RDAGVVTKLRKAGAII GKASLS+WS FRS   P+GW+AR GQ
Sbjct: 121 QLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEQPNGWSARGGQ 180

Query: 181 GREPYTLGDPCGSSSGSAISVSANMAAVAIGTETDGSLLCPAAYNSVVAIKPTVGLTSRA 240
           G+ PYT+G+PCGSSSGSAISV+ANM  V++GTETDGS+LCP+  NSVV IKPTVGLTSRA
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVIPISTRQDTVGPIGRTVADAAYVLDAIVGYDAFDQATYCRSQFIPKDGYGQFLKADGL 300
           GV+PIS RQDTVGPI RTVADAAYVLDAI G D +D +T   S+++PK GYGQFLK DGL
Sbjct: 241 GVVPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYVPKGGYGQFLKEDGL 300

Query: 301 RGKRLGIVED-FSIGIRPSI-VQAFQEALQILSKRGAILVSNLKIRDFKTIANPGESAKV 360
           +GKR+GIV   +  G      + AF++  + L + GAILV NL I  F  I     S + 
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINSFHVITG-SSSGEW 360

Query: 361 TALLNEFRVSVNAYLEGLVSSPVQSLSGVIEFNKQHVHLEEMAKYGQDFLLEANRTNGIG 420
           TA+L EF++S+N YL+ LV+SP++SLS  IEFN+++  LE++ +YGQ+  LEA  T GIG
Sbjct: 361 TAVLAEFKISINVYLKQLVASPIRSLSDAIEFNRKNSKLEKLKEYGQELFLEAEATKGIG 420

Query: 421 LG--AALSKLRALSREGFEKTMTDNRLDAIIAPSEIICSFLAIGGLPGITVPAGYTPTGK 480
               AAL++L  LS+EGFE+ M  N+LDAI AP  +I  FLAIGG PG++VPAGY P G 
Sbjct: 421 GAEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISHFLAIGGFPGVSVPAGYNPQGL 480

Query: 481 PFGFYFGGLRGYEPRLIEIAYDFEQATKTRKPPPLSK 513
           PFG  FGGL+G+EPRLIEIAY FE  T  RK P L +
Sbjct: 481 PFGIGFGGLKGFEPRLIEIAYGFEHLTMGRKSPSLGR 514

BLAST of Spg001276 vs. ExPASy TrEMBL
Match: E5GC08 (Amidase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 8.3e-163
Identity = 318/507 (62.72%), Postives = 384/507 (75.74%), Query Frame = 0

Query: 12  LLLILVAVSAIESGSVG--TGFSIEEATLKDLRSAFFNQTNLTSRRLVEFYIEQIRKYNA 71
           LL +L  +S   SGS    T FSIEEATLKD + AF+ Q  LTSR+LVEFY+EQ+R+ N 
Sbjct: 2   LLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFY-QNKLTSRQLVEFYLEQVRRLNP 61

Query: 72  SLGGVIEVNPDALDLADAADRKRRASTTPSALPLLHGIPVLVKDNIATKDKLNTTAGSFA 131
            L G+IEVNPDAL+ A  AD KR+ S+  S  P LHGIPVLVKDNIATKDKLNTTAGSFA
Sbjct: 62  ILKGIIEVNPDALNQASQADLKRKRSSLRSLSP-LHGIPVLVKDNIATKDKLNTTAGSFA 121

Query: 132 LLSSVVARDAGVVTKLRKAGAIILGKASLSQWSGFRSSNAPSGWNARSGQGREPYTLGDP 191
           LL S+V RDAGVVTKLRKAGAII GKASLS+WSGFRS   P+GW+AR GQG+ PYT+G+P
Sbjct: 122 LLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQGKNPYTMGEP 181

Query: 192 CGSSSGSAISVSANMAAVAIGTETDGSLLCPAAYNSVVAIKPTVGLTSRAGVIPISTRQD 251
           CGSSSGSAISV+ANM  V++GTETDGS+LCP+  NSVV IKPTVGLTSRAGV+PIS RQD
Sbjct: 182 CGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQD 241

Query: 252 TVGPIGRTVADAAYVLDAIVGYDAFDQATYCRSQFIPKDGYGQFLKADGLRGKRLGIVED 311
           TVGPI RTVADAAYVLDAI G D +D +T   S++IP+ GYGQFL+A+GL+GKR+GIV  
Sbjct: 242 TVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGLKGKRIGIVRK 301

Query: 312 -FSIGIRPSI-VQAFQEALQILSKRGAILVSNLKIRDFKTIANPGESAKVTALLNEFRVS 371
            +  G      + AF++  + L + GAILV NL I  F  I     S + TALL EF++S
Sbjct: 302 LYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITG-SSSGEWTALLAEFKIS 361

Query: 372 VNAYLEGLVSSPVQSLSGVIEFNKQHVHLEEMAKYGQDFLLEANRTNGIGLG--AALSKL 431
           +NAYL+ LV+SP++SLS  IEFNK++  LE++ +YGQ+  LEA  T GIG    AAL++L
Sbjct: 362 LNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGGAEKAALARL 421

Query: 432 RALSREGFEKTMTDNRLDAIIAPSEIICSFLAIGGLPGITVPAGYTPTGKPFGFYFGGLR 491
             LS+EGFE+ M  N+LDAI AP  +I  FLAIGG PG++VPAGY P G PFG  FGGL+
Sbjct: 422 AKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLPFGIGFGGLK 481

Query: 492 GYEPRLIEIAYDFEQATKTRKPPPLSK 513
           G++PRLIEIAY FE  T  RK P L +
Sbjct: 482 GFDPRLIEIAYGFEHLTMGRKSPSLGR 505

BLAST of Spg001276 vs. TAIR 10
Match: AT4G34880.1 (Amidase family protein )

HSP 1 Score: 429.1 bits (1102), Expect = 5.1e-120
Identity = 251/506 (49.60%), Postives = 325/506 (64.23%), Query Frame = 0

Query: 13  LLILVAVSAIESGSVGTG--------FSIEEATLKDLRSAFFNQTNLTSRRLVEFYIEQI 72
           LLIL+ +S     SVG+         FSI+EAT++D+R A FN+  LTS++LVE Y+E I
Sbjct: 12  LLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVA-FNEKRLTSKQLVELYLEAI 71

Query: 73  RKYNASLGGVIEVNPDALDLADAADRKRRASTTPSALPLLHGIPVLVKDNIATKDKLNTT 132
            K N  L  VIE NPDAL  A+ ADR+R    T + LP+LHG+PVL+KD+I+TKDKLNTT
Sbjct: 72  SKLNPILHAVIETNPDALIQAEIADRERDLKNT-TKLPILHGVPVLLKDSISTKDKLNTT 131

Query: 133 AGSFALLSSVVARDAGVVTKLRKAGAIILGKASLSQWSGFRSSNAPSGWNARSGQGREPY 192
           AGSFALL SVVARDAGVV +LR++GA+ILGKASLS+W+ FRS + P GW+A S       
Sbjct: 132 AGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSAPS------- 191

Query: 193 TLGDPCGSSSGSAISVSANMAAVAIGTETDGSLLCPAAYNSVVAIKPTVGLTSRAGVIPI 252
                                                  NSVV IKP+VGLTSRAGV+PI
Sbjct: 192 --------------------------------------QNSVVGIKPSVGLTSRAGVVPI 251

Query: 253 STRQDTVGPIGRTVADAAYVLDAIVGYDAFDQATYCRSQFIPKDGYGQFLKADGLRGKRL 312
           S RQD++GPI RTV+DA ++LDAIVGYD  D+AT   S+FIP+ GY QFL   GL+GKRL
Sbjct: 252 SLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLTTSGLKGKRL 311

Query: 313 GIVEDFSIGIRPSIVQAFQEALQILSKRGAILVSNLKIRDFKTIANPGESAKVTALLNEF 372
           GIV   S              ++ L + GAI+++NL I + + I    +S +  ALL EF
Sbjct: 312 GIVMKHS--------SLLDHHIKTLRREGAIVINNLTIPNIEVIVGGTDSGEEIALLAEF 371

Query: 373 RVSVNAYLEGLVSSPVQSLSGVIEFNKQHVHLEEMAKYGQDFLLEANRTNGIG--LGAAL 432
           ++S+NAYL+ LV SPV+SL+ VI +N++    E++ ++GQ+  L A  T+G+G     AL
Sbjct: 372 KMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKEKTAL 431

Query: 433 SKLRALSREGFEKTMTDNRLDAIIAPSEIICSFLAIGGLPGITVPAGYTPTGKPFGFYFG 492
            K++ LSR G EK + +N+LDAI+     + S LAIGG PGI VPAGY   G P+G  FG
Sbjct: 432 QKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYGISFG 462

Query: 493 GLRGYEPRLIEIAYDFEQATKTRKPP 509
           GLR  EP+LIEIA+ FEQAT  RKPP
Sbjct: 492 GLRFSEPKLIEIAFAFEQATLIRKPP 462

BLAST of Spg001276 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 127.5 bits (319), Expect = 3.1e-29
Identity = 157/567 (27.69%), Postives = 246/567 (43.39%), Query Frame = 0

Query: 3   PKSLS--PYLHLLLILVAVSAIESGSVGTGF--SIEEATLKDLRSAFFNQTNLTSRRLVE 62
           P+SLS  P     +    VSA  S ++ T      +   L   RS    +T  T+  + +
Sbjct: 8   PRSLSLLPLRRFQISKTIVSAASSKTIDTSVISPPQSQILTTRRSLLSGET--TAVEIAK 67

Query: 63  FYIEQIRKYNASLGGVIEVNPDALDLADAADRKRRASTTPSALPLLHGIPVLVKDNIATK 122
            Y+ +IR     L   + V+ +   L DA +  +R +      PL  G+ + VKDNI T+
Sbjct: 68  SYLSRIRLTEPQLKCFLHVSENV--LKDAQEIDQRIAKGEELGPLA-GVLIGVKDNICTQ 127

Query: 123 DKLNTTAGSFALLSSVVARDAGVVTKLRKAGAIILGKASLSQWSGFRSSNAPSGWNARSG 182
             + +TA S  L       DA  V K+++ G I++GK ++ ++ G  S+   S +   + 
Sbjct: 128 G-MPSTAASRILEHYRPPFDATAVKKIKELGGIVVGKTNMDEF-GMGSTTEASAFQVTA- 187

Query: 183 QGREPYTLGD-PCGSSSGSAISVSANMAAVAIGTETDGSLLCPAAYNSVVAIKPTVGLTS 242
               P+ L   P GSS GSA +V+A    V++G++T GS+  PA++  VV +KPT G  S
Sbjct: 188 ---NPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCGVVGLKPTYGRVS 247

Query: 243 RAGVIPISTRQDTVGPIGRTVADAAYVLDAIVGYDAFDQATYCRSQFIPKDGYGQFLKAD 302
           R G++  ++  D +G  G TVADA  +L AI GYD FD  +    Q +P +   QFL  D
Sbjct: 248 RFGLMAYASSLDVIGCFGSTVADAGMLLHAISGYDRFDSTS--SKQDVP-EFQSQFLSVD 307

Query: 303 -----GLRGKRLGIV-EDFSIGIRPSIVQAFQEALQILSKRGAILVSNLKIRDFKTIANP 362
                 L G ++GI+ E    G+   +  A QEA   L   G IL               
Sbjct: 308 HFESKPLNGVKVGIIRETLEDGVDSGVRSATQEAASHLEALGCILTE------------- 367

Query: 363 GESAKVTALLNEFRVSVNAYLEGLVSSPVQSLSGV--IEFNKQHVHLEEMAKY-----GQ 422
                    L  F + + AY     S    +LS    + +  Q V  EE+ K      G+
Sbjct: 368 -------VSLPSFSLGLPAYYVIASSESSSNLSRYDGVRYGNQ-VMAEELNKLYECSRGE 427

Query: 423 DFLLEANRTNGIGLGA-----------ALSKLRALSREGFEKTMTDNRLDAIIAPS---- 482
            F  E      +G  A              ++R L R+ F+  +  N  D +I+P+    
Sbjct: 428 GFGGEVKMRILMGTYALSAGYYDAYYKRAQQVRTLIRKDFKAALEQN--DILISPAAPSA 487

Query: 483 -----------------EIICSFLAIGGLPGITVPAGYT---PTGKPFGFYFGGLRGYEP 515
                            +I+   + + GLP + +P G     P+G P G    G    E 
Sbjct: 488 AYKIGEKKDDPLAMYAGDIMTVNVNLAGLPAMVLPCGLVEGGPSGLPVGLQMIGAAFDEE 537

BLAST of Spg001276 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 104.4 bits (259), Expect = 2.9e-22
Identity = 84/232 (36.21%), Postives = 116/232 (50.00%), Query Frame = 0

Query: 51  LTSRRLVEFYIEQIRKYNASLGGVIEVNPD-ALDLADAADRKRRASTTPSALPLLHGIPV 110
           +TS+ LV  Y++Q+++YN  L  V+    + A   A  AD      T    L  LHGIP 
Sbjct: 217 ITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEADDLLSQGT---YLGPLHGIPY 276

Query: 111 LVKDNIATKDKLNTTAGSFALLSSVVARDAGVVTKLRKAGAIILGKASLSQ------WSG 170
            +KD +A      TT GS +     +  +A V  +L+ +GA+++ K           W G
Sbjct: 277 GLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTGSMAYDDIWFG 336

Query: 171 FRSSNAPSGWNARSGQGREPYTLGDPCGSSSGSAISVSANMAAVAIGTETDGSLLCPAAY 230
            R+ N    WN       E ++     GSS+G A S SA M   AIG+ET GS+  PAA 
Sbjct: 337 GRTRNP---WNI------EEFS----TGSSAGPAASTSAGMVPFAIGSETAGSMTYPAAR 396

Query: 231 NSVVAIKPTVGLTSRAGVIPISTRQDTVGPIGRTVADAAYVLDAIVGYDAFD 276
             + A++PT G   R GV+ IS   D +GP  RT AD A +LDAI G D  D
Sbjct: 397 CGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDD 431

BLAST of Spg001276 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 94.0 bits (232), Expect = 3.9e-19
Identity = 81/232 (34.91%), Postives = 113/232 (48.71%), Query Frame = 0

Query: 51  LTSRRLVEFYIEQIRKYNASLGGVIEVNPD-ALDLADAADRKRRASTTPSALPLLHGIPV 110
           +TS+ LV  Y++Q+++YN  L  V+    + A   A  AD      T    L  LHGIP 
Sbjct: 217 ITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEADDLLSQGT---YLGPLHGIPY 276

Query: 111 LVKDNIATKDKLNTTAGSFALLSSVVARDAGVVTKLRKAGAIILGKASLSQ------WSG 170
            +KD +A      TT GS +     +  +A V  +L+ +GA+++ K           W G
Sbjct: 277 GLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTGSMAYDDIWFG 336

Query: 171 FRSSNAPSGWNARSGQGREPYTLGDPCGSSSGSAISVSANMAAVAIGTETDGSLLCPAAY 230
            R+ N    WN       E ++     GSS+G A S SA       G+ET GS+  PAA 
Sbjct: 337 GRTRNP---WNI------EEFS----TGSSAGPAASTSA-------GSETAGSMTYPAAR 396

Query: 231 NSVVAIKPTVGLTSRAGVIPISTRQDTVGPIGRTVADAAYVLDAIVGYDAFD 276
             + A++PT G   R GV+ IS   D +GP  RT AD A +LDAI G D  D
Sbjct: 397 CGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDD 424

BLAST of Spg001276 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 83.6 bits (205), Expect = 5.2e-16
Identity = 104/449 (23.16%), Postives = 188/449 (41.87%), Query Frame = 0

Query: 80  DALDLADAADRKRRASTTPSALPLLHGIPVLVKDNIATKDKLNTTAGSFALLSSVVARDA 139
           DA ++   A+   R     + + +L GI V +KD+I           ++      V +D+
Sbjct: 173 DANEVIKQAEASTRRFEQGNPISVLDGIFVTIKDDIDCLPHPTNGGTTWLHEDRSVEKDS 232

Query: 140 GVVTKLRKAGAIILGKASLSQWSGFRSSNAPSGWNARSGQGREPYTLGDPCGSSSGSAIS 199
            VV+KLR  GAI+LGKA++ +     + N  +    R+    + YT     GSSSGSA  
Sbjct: 233 AVVSKLRSCGAILLGKANMHELGMGTTGNNSNYGTTRNPHDPKRYT----GGSSSGSAAI 292

Query: 200 VSANMAAVAIGTETDGSLLCPAAYNSVVAIKPTVGLTSRAGVIPISTRQDTVGPIGRTVA 259
           V+A + + A+GT+  GS+  P+A   +  +K T G T   G +      + +GP+  ++ 
Sbjct: 293 VAAGLCSAALGTDGGGSVRIPSALCGITGLKTTYGRTDMTGSLCEGGTVEIIGPLASSLE 352

Query: 260 DAAYVLDAIVGYDAFDQ-----ATYCRSQFIPKDGYGQFLKADGLRGKRLGIVEDFSIGI 319
           DA  V  AI+G  + D+     +  C  + +  +G      ++ +   RLG    +   +
Sbjct: 353 DAFLVYAAILGSSSADRYNLKPSPPCFPKLLSHNG------SNAIGSLRLGKYTKWFNDV 412

Query: 320 RPS-IVQAFQEALQILSKRGAILVSNLKIRDFKTIANPGESAKVTALLNEFRVSVNAYLE 379
             S I    ++ L++LS      V  + + + + +     +A V ++ +    S+  Y E
Sbjct: 413 SSSDISDKCEDILKLLSNNHGCKVVEIVVPELEEM----RAAHVISIGSPTLSSLTPYCE 472

Query: 380 GLVSSPV-----------QSLSGVIEFNKQHVHLEEMAKYGQDFL-LEANRTNGIGLGAA 439
              +S +           +S S       Q +    M  +   F  ++   T   G+ A 
Sbjct: 473 AGKNSKLSYDTRTSFAIFRSFSASDYIAAQCLRRRLMEYHLNIFKDVDVIVTPTTGMTAP 532

Query: 440 LSKLRALSREGFEKTMTDNRLDAIIAPSEIICSFLAIGGLPGITVPAGYTPTGKPFGFYF 499
           +    AL        +T + +  ++A + +        G P I+VP GY   G P G   
Sbjct: 533 VIPPDALKNGETNIQVTTDLMRFVLAANLL--------GFPAISVPVGYDKEGLPIGLQI 592

Query: 500 GGLRGYEPRLIEIAYDFEQATKTRKPPPL 511
            G    E  ++ +A   E+     K P +
Sbjct: 593 MGRPWAEATVLGLAAAVEELAPVTKKPAI 599

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146229.14.5e-18769.67putative amidase C869.01 [Momordica charantia][more]
XP_008457659.11.6e-16361.66PREDICTED: putative amidase C869.01 isoform X1 [Cucumis melo] >KAA0045712.1 puta... [more]
XP_004147024.34.5e-16361.32probable amidase At4g34880 [Cucumis sativus][more]
ADN34007.11.7e-16262.72amidase [Cucumis melo subsp. melo][more]
XP_008457661.11.5e-16161.78PREDICTED: putative amidase C869.01 [Cucumis melo] >ADN34008.1 amidase [Cucumis ... [more]
Match NameE-valueIdentityDescription
A0A1P8B7608.6e-14155.42Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=... [more]
Q9URY41.2e-7036.27Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) ... [more]
D4B3C82.7e-6535.23Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS ... [more]
B0JSX37.3e-3929.17Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Microcystis aeruginosa (strain ... [more]
B0KBN43.6e-3828.80Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter pseudethanol... [more]
Match NameE-valueIdentityDescription
A0A6J1CXI82.2e-18769.67putative amidase C869.01 OS=Momordica charantia OX=3673 GN=LOC111015494 PE=4 SV=... [more]
A0A5D3BN387.5e-16461.66Putative amidase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3C7A87.5e-16461.66putative amidase C869.01 isoform X1 OS=Cucumis melo OX=3656 GN=LOC107990287 PE=4... [more]
A0A0A0LLK06.4e-16361.12Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264040 PE=... [more]
E5GC088.3e-16362.72Amidase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G34880.15.1e-12049.60Amidase family protein [more]
AT3G25660.13.1e-2927.69Amidase family protein [more]
AT5G07360.12.9e-2236.21Amidase family protein [more]
AT5G07360.23.9e-1934.91Amidase family protein [more]
AT5G64440.15.2e-1623.16fatty acid amide hydrolase [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036928Amidase signature (AS) superfamilyGENE3D3.90.1300.10Amidase signature (AS) domaincoord: 22..510
e-value: 1.9E-139
score: 467.9
IPR036928Amidase signature (AS) superfamilySUPERFAMILY75304Amidase signature (AS) enzymescoord: 36..509
IPR023631Amidase signature domainPFAMPF01425Amidasecoord: 55..450
e-value: 1.2E-67
score: 228.9
NoneNo IPR availablePANTHERPTHR42678AMIDASEcoord: 16..511
NoneNo IPR availablePANTHERPTHR42678:SF19C869.01-LIKE PROTEIN, PUTATIVE-RELATEDcoord: 16..511

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg001276.1Spg001276.1mRNA