Spg001147 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg001147
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionProtein kinase domain-containing protein
Locationscaffold8: 43716715 .. 43720043 (+)
RNA-Seq ExpressionSpg001147
SyntenySpg001147
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTCAAGAGAACTGTACAACAATCTAATGCAATCTCCTCTTCCCTTTTGCCCATTTTTCACGCTCCAAACTTCAAATCAAAGACCCACCGATTCTCAAATCTGCCCTACGCGCCACTCCGTTCCGATTTCCTCTTCTGTGAGCCCATTCTCCATGTAGTTGCAGTGAACGGACTTTGATACGGCGGAGCCATGGCGACGGCATTGGAGTGCTGGTCGAGTAGGGCTAGTACCGATGAGGATTTGGTGGAGCAGGTGCTGATGAGGACTCAGGATCGATCGGAAGGCTCCAAGCCGGAGAGTTCGTTTGCCGTTGGAGCGAAGGAGTCGTCGGCGATGCAGAAACGGTTGCAGAGATTGAGTCGGAATGTGTCCGAGGCGGTGGCGTCGATTAAAAACTCGTTGAATCTGGACTCTGCTCGCGATCCCTCGCCTACGAAGACCGAGGGGTCTAAGAAGGCTGTTTGGGGGAGTGTTGTACGGAATCTTACTCAGCTCTATCCTGGCAGTCAGTTGCCGGAAAAGCTCGTATCTAACATTCGCAAGCATTATGATTCGTTGCCTCTCAGGTTTGGACTCTGATTAGTAATGCTGTAAGTTCAAAGTTGTGTAAACTGGAGAGATGGATTGAAAAAGAAAATCACGAACTGATAGGAGCTAGTCTAGTAACTAAGTATTGTGATGGAAGATAGATCAGATATCTATTTGGTCAATGTTTGTGGTCAATGTTTGCTAATCATCTTATAGTAGAAGACTTAAACTAGGTTGAGTTTATCGATGTCACCTTGCGTTGTTCCCTGTTCTGACTGCTGTATATTCTTCTTTATGTTGTTTTGTTGGTATGAACAGTTATGCTCAGGCGGGGTTTGACATGAAAGATGTCTTTCTCCACATTAAATTGATAGAGCAGGCATCTGTTTATGATCACCCTGCTATCTTATTTCAAGAAGTGACTAATCATGACGTTCAGAAACCTACAATGAAGCTCACATTTGCTTGCAACTCTTCAATTTCATGGTCGACGATGTCTGGAGCCTTGGAGAGTGCTGACATTCGCTGTGAGAAGATACAGATTTTTGAGAAGAAAAAATTTACTCTTGGAGTCGTTCTTTTTGTGAACCTAGATGCCCAAGAGAAAGTCTTCAAATCCAAAGTTGAAAACGCTCTAAAATTGGCTATTAAGAAGCCAAAAACCAATACAGTGAAACTCCCATTTGGATTTTGTGGATGCCAAGAAGGTAACACTCGAGGGAAAGATTTGAGAGAAATCGAGGAGGACGCTGTTGAACAAAATTGCAGAAGTGGTTTTGAAAACTCGAATTTGAATGAAAATTTACAGATAGAAATGCCCTTATCTACTTCATCATTTGCTGTATTTGTCGATGAATGGCAAACGGTCCAATCTGGTGGGCATGAGCTGGGTAAATGGCTGCTGAGCTCTGAGAATCTAGAGCTCGTTGATCAGATTGGACCCAACTCGTTCAAGGGAGTCTACAAGGGCAAAAGAGTAGCTATAGAGAAGATTAAAGGGTGTGAAAAGGGAGTTTCTTACAAGTTTGAGCTTCGAAAAGACTTGTTGGAGCTGATGACATGTGGGCACAAGAACATTCTAATGTTCTATGGTGTCTGTATTGACGAAAATCATGGTTTGTGTGTTGTAACCAAACTAATGGAGGGCGGATCAGTCCATGAATTGATGCTGAAAAACAAAAGGCTTCAGACGAAAGAAATAACCAGGATTGCTATCGATGTTGTAGAAGGGATCAAATTCATGAATGACCATGGTGTTGCCTATCGAGATCTGAACACACAGAGAATTTTGTTAGATAAGAACGGCAATGCTTGTTTGGGTGACATGGGCATACTCACTGCATGCAAAAACTTGGGGGAGGCAATGGAGTACGAGACCGATGGCTATCGATGGCTAGCTCCCGAGGTTTGTTTCTATTACTCTGATTAGACTTGGTATTATCTAAAGCAAGTTTCAATTATAGTATGCATTGAAAAGAACCTGATATAGATCTCTCTGCTTTTAGCTGAACAACGCAATATCTTCTCGCTTTTTTGTGGTGTAATTAGTGTTTGGATTCATTACATTAAACCGCATGGGATGAGTCCTTTTTTCAATTAAAGAGACTTTATGTCACTTCCTCCATGATGGTCTAGTGTGTGTAGCATAGAGTCATGATCGAAGATGCTTATCAGTATTTTGTTTTTATTTTTCTAGAAACTCTCATATTTTTTAGACCATTAAAATTAGATTCCACTGCACCCATCTGTTTGGCCGCAACTTCGAGTTGTTGCCACTTTCTACTCACTGGTTCAATTTGTGGAGCCCGCCATCTTAAAGCTCCTTTAGAGAGAATCTGCTCTTTTGTTCCACGGAATTTGAAATTCCTGTAGATCTCATCTCATTACTATCATTTACTTGTCAGCCTCTTTATATATTTCCCAATTTGATTGGTAAGTATTTAAGTAATGACAGAAGGGTGTTATTAATTTGTATTTCTTGGCTCTATCTGGTTTGGCACAGATTATTGCTGGCGACCCGGAGAGTGTTAACGAGACATGGATGAGTAACGTATACAGCTTTGGAATGGTAATATGGGAGATGGTGACCGGTGAGGCAGCGTATAGTGCATATTCGCCAGTGCAGGCTGCAGTCGGTATAGCTGCTTGTGGTCTTAGACCTGATGTTCCAAAGGACTGCCCATCCATCCTGAAATCTCTGATGATCAAATGCTGGAACAATTGCCCAAATAAGCGCCCCCAGTTCTCTGAAATTTTATCAGTGCTGCTGGACAACAACAATAACAACCATAGATAAAGTAAGTATATTCCTCTTAATCTAAATGGATTCTAAGCCTACTCTTATGAAATTATTGTAAAGGGTTCCTTTTTCATGTAGAAGAAATGCTCTGCGTTCAATGTAGATGTGAGAAGTTTGTGTTTTTCAGATATTCATCCGATTACTTCTCATGGATTAAAAAACAAAAAACAAAAAAAAAAACTAATCTCCTTTCGATTCTGAACTATAGGAAAATCAACCCTCTTACCCAGTTGCCACCACCCAGAGGGGGGAAGGAGACAAAGAAAGATCTTTCTTTCCCCTTTCTATTTATTATTGTTATTATTATTTTTTCTACAATGGCTTTGACGTCATCATGTAAGACCATTTAGTTCTTTGAATGACAAGAAGTTCCAAGGCTGTATTTTTTAAGGTGGATAATGGATTCTTAAGAGAGATTATTTGATGTAAGGCCGAGACAGATTCTAACTAGCTAAGACAAGTTAATATAAATTTGATCTTTCTAATATATCATTAACATGA

mRNA sequence

ATGGCGACGGCATTGGAGTGCTGGTCGAGTAGGGCTAGTACCGATGAGGATTTGGTGGAGCAGGTGCTGATGAGGACTCAGGATCGATCGGAAGGCTCCAAGCCGGAGAGTTCGTTTGCCGTTGGAGCGAAGGAGTCGTCGGCGATGCAGAAACGGTTGCAGAGATTGAGTCGGAATGTGTCCGAGGCGGTGGCGTCGATTAAAAACTCGTTGAATCTGGACTCTGCTCGCGATCCCTCGCCTACGAAGACCGAGGGGTCTAAGAAGGCTGTTTGGGGGAGTGTTGTACGGAATCTTACTCAGCTCTATCCTGGCAGTCAGTTGCCGGAAAAGCTCGTATCTAACATTCGCAAGCATTATGATTCGTTGCCTCTCAGTTATGCTCAGGCGGGGTTTGACATGAAAGATGTCTTTCTCCACATTAAATTGATAGAGCAGGCATCTGTTTATGATCACCCTGCTATCTTATTTCAAGAAGTGACTAATCATGACGTTCAGAAACCTACAATGAAGCTCACATTTGCTTGCAACTCTTCAATTTCATGGTCGACGATGTCTGGAGCCTTGGAGAGTGCTGACATTCGCTGTGAGAAGATACAGATTTTTGAGAAGAAAAAATTTACTCTTGGAGTCGTTCTTTTTGTGAACCTAGATGCCCAAGAGAAAGTCTTCAAATCCAAAGTTGAAAACGCTCTAAAATTGGCTATTAAGAAGCCAAAAACCAATACAGTGAAACTCCCATTTGGATTTTGTGGATGCCAAGAAGGTAACACTCGAGGGAAAGATTTGAGAGAAATCGAGGAGGACGCTGTTGAACAAAATTGCAGAAGTGGTTTTGAAAACTCGAATTTGAATGAAAATTTACAGATAGAAATGCCCTTATCTACTTCATCATTTGCTGTATTTGTCGATGAATGGCAAACGGTCCAATCTGGTGGGCATGAGCTGGGTAAATGGCTGCTGAGCTCTGAGAATCTAGAGCTCGTTGATCAGATTGGACCCAACTCGTTCAAGGGAGTCTACAAGGGCAAAAGAGTAGCTATAGAGAAGATTAAAGGGTGTGAAAAGGGAGTTTCTTACAAGTTTGAGCTTCGAAAAGACTTGTTGGAGCTGATGACATGTGGGCACAAGAACATTCTAATGTTCTATGGTGTCTGTATTGACGAAAATCATGGTTTGTGTGTTGTAACCAAACTAATGGAGGGCGGATCAGTCCATGAATTGATGCTGAAAAACAAAAGGCTTCAGACGAAAGAAATAACCAGGATTGCTATCGATGTTGTAGAAGGGATCAAATTCATGAATGACCATGGTGTTGCCTATCGAGATCTGAACACACAGAGAATTTTGTTAGATAAGAACGGCAATGCTTGTTTGGGTGACATGGGCATACTCACTGCATGCAAAAACTTGGGGGAGGCAATGGAGTACGAGACCGATGGCTATCGATGGCTAGCTCCCGAGATTATTGCTGGCGACCCGGAGAGTGTTAACGAGACATGGATGAGTAACGTATACAGCTTTGGAATGGTAATATGGGAGATGGTGACCGGTGAGGCAGCGTATAGTGCATATTCGCCAGTGCAGGCTGCAGTCGGTATAGCTGCTTGTGGTCTTAGACCTGATGTTCCAAAGGACTGCCCATCCATCCTGAAATCTCTGATGATCAAATGCTGGAACAATTGCCCAAATAAGCGCCCCCAGTTCTCTGAAATTTTATCAGTGCTGCTGGACAACAACAATAACAACCATAGATAA

Coding sequence (CDS)

ATGGCGACGGCATTGGAGTGCTGGTCGAGTAGGGCTAGTACCGATGAGGATTTGGTGGAGCAGGTGCTGATGAGGACTCAGGATCGATCGGAAGGCTCCAAGCCGGAGAGTTCGTTTGCCGTTGGAGCGAAGGAGTCGTCGGCGATGCAGAAACGGTTGCAGAGATTGAGTCGGAATGTGTCCGAGGCGGTGGCGTCGATTAAAAACTCGTTGAATCTGGACTCTGCTCGCGATCCCTCGCCTACGAAGACCGAGGGGTCTAAGAAGGCTGTTTGGGGGAGTGTTGTACGGAATCTTACTCAGCTCTATCCTGGCAGTCAGTTGCCGGAAAAGCTCGTATCTAACATTCGCAAGCATTATGATTCGTTGCCTCTCAGTTATGCTCAGGCGGGGTTTGACATGAAAGATGTCTTTCTCCACATTAAATTGATAGAGCAGGCATCTGTTTATGATCACCCTGCTATCTTATTTCAAGAAGTGACTAATCATGACGTTCAGAAACCTACAATGAAGCTCACATTTGCTTGCAACTCTTCAATTTCATGGTCGACGATGTCTGGAGCCTTGGAGAGTGCTGACATTCGCTGTGAGAAGATACAGATTTTTGAGAAGAAAAAATTTACTCTTGGAGTCGTTCTTTTTGTGAACCTAGATGCCCAAGAGAAAGTCTTCAAATCCAAAGTTGAAAACGCTCTAAAATTGGCTATTAAGAAGCCAAAAACCAATACAGTGAAACTCCCATTTGGATTTTGTGGATGCCAAGAAGGTAACACTCGAGGGAAAGATTTGAGAGAAATCGAGGAGGACGCTGTTGAACAAAATTGCAGAAGTGGTTTTGAAAACTCGAATTTGAATGAAAATTTACAGATAGAAATGCCCTTATCTACTTCATCATTTGCTGTATTTGTCGATGAATGGCAAACGGTCCAATCTGGTGGGCATGAGCTGGGTAAATGGCTGCTGAGCTCTGAGAATCTAGAGCTCGTTGATCAGATTGGACCCAACTCGTTCAAGGGAGTCTACAAGGGCAAAAGAGTAGCTATAGAGAAGATTAAAGGGTGTGAAAAGGGAGTTTCTTACAAGTTTGAGCTTCGAAAAGACTTGTTGGAGCTGATGACATGTGGGCACAAGAACATTCTAATGTTCTATGGTGTCTGTATTGACGAAAATCATGGTTTGTGTGTTGTAACCAAACTAATGGAGGGCGGATCAGTCCATGAATTGATGCTGAAAAACAAAAGGCTTCAGACGAAAGAAATAACCAGGATTGCTATCGATGTTGTAGAAGGGATCAAATTCATGAATGACCATGGTGTTGCCTATCGAGATCTGAACACACAGAGAATTTTGTTAGATAAGAACGGCAATGCTTGTTTGGGTGACATGGGCATACTCACTGCATGCAAAAACTTGGGGGAGGCAATGGAGTACGAGACCGATGGCTATCGATGGCTAGCTCCCGAGATTATTGCTGGCGACCCGGAGAGTGTTAACGAGACATGGATGAGTAACGTATACAGCTTTGGAATGGTAATATGGGAGATGGTGACCGGTGAGGCAGCGTATAGTGCATATTCGCCAGTGCAGGCTGCAGTCGGTATAGCTGCTTGTGGTCTTAGACCTGATGTTCCAAAGGACTGCCCATCCATCCTGAAATCTCTGATGATCAAATGCTGGAACAATTGCCCAAATAAGCGCCCCCAGTTCTCTGAAATTTTATCAGTGCTGCTGGACAACAACAATAACAACCATAGATAA

Protein sequence

MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNVSEAVASIKNSLNLDSARDPSPTKTEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHYDSLPLSYAQAGFDMKDVFLHIKLIEQASVYDHPAILFQEVTNHDVQKPTMKLTFACNSSISWSTMSGALESADIRCEKIQIFEKKKFTLGVVLFVNLDAQEKVFKSKVENALKLAIKKPKTNTVKLPFGFCGCQEGNTRGKDLREIEEDAVEQNCRSGFENSNLNENLQIEMPLSTSSFAVFVDEWQTVQSGGHELGKWLLSSENLELVDQIGPNSFKGVYKGKRVAIEKIKGCEKGVSYKFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLQTKEITRIAIDVVEGIKFMNDHGVAYRDLNTQRILLDKNGNACLGDMGILTACKNLGEAMEYETDGYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAYSAYSPVQAAVGIAACGLRPDVPKDCPSILKSLMIKCWNNCPNKRPQFSEILSVLLDNNNNNHR
Homology
BLAST of Spg001147 vs. NCBI nr
Match: KAG7010490.1 (Serine/threonine-protein kinase STY46 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1122.8 bits (2903), Expect = 0.0e+00
Identity = 552/585 (94.36%), Postives = 571/585 (97.61%), Query Frame = 0

Query: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV 60
           MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESS AVG KESSAMQKRLQR SRNV
Sbjct: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSLAVGEKESSAMQKRLQRFSRNV 60

Query: 61  SEAVASIKNSLNLDSARDPSPTKTEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120
           SEAVAS+KNSLNLDS RDPSPTKTEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY
Sbjct: 61  SEAVASLKNSLNLDSVRDPSPTKTEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120

Query: 121 DSLPLSYAQAGFDMKDVFLHIKLIEQASVYDHPAILFQEVTNHDVQKPTMKLTFACNSSI 180
           DSLPLSYAQAGF+MKDVFLHIKLIEQASVYDHPAILFQEVTNHDVQKPT+KLTFACNSS+
Sbjct: 121 DSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNHDVQKPTIKLTFACNSSV 180

Query: 181 SWSTMSGALESADIRCEKIQIFEKKKFTLGVVLFVNLDAQEKVFKSKVENALKLAIKKPK 240
           SWS MSGALESA IRCEKIQIFEKKKF+LGV+LFVNLDAQEK+FKSKVENALKLAIKKPK
Sbjct: 181 SWSVMSGALESAGIRCEKIQIFEKKKFSLGVILFVNLDAQEKLFKSKVENALKLAIKKPK 240

Query: 241 TNTVKLPFGFCGCQEGNTRGKDLREIEEDAVEQNCRSGFENSNLNENLQIEMPLSTSSFA 300
           TNTVKLPFGFCGCQEGNT GKDLREIEEDAV+QNCRSGFENSNLNENLQIEMPLSTSSF 
Sbjct: 241 TNTVKLPFGFCGCQEGNTGGKDLREIEEDAVDQNCRSGFENSNLNENLQIEMPLSTSSFT 300

Query: 301 VFVDEWQTVQSGGHELGKWLLSSENLELVDQIGPNSFKGVYKGKRVAIEKIKGCEKGVSY 360
           V VDEWQTVQSGGHELGKWLLSSENLE  DQIGPNSFKGVYKG+RVAIEKIKGCEKGVSY
Sbjct: 301 VTVDEWQTVQSGGHELGKWLLSSENLEFTDQIGPNSFKGVYKGRRVAIEKIKGCEKGVSY 360

Query: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLQTKEI 420
           KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLQ KEI
Sbjct: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLQMKEI 420

Query: 421 TRIAIDVVEGIKFMNDHGVAYRDLNTQRILLDKNGNACLGDMGILTACKNLGEAMEYETD 480
           TRIA+DVVEGIKF+NDHGVAYRDLNTQRILLDKNGNACLGDMGILTAC+NLGEAMEYETD
Sbjct: 421 TRIAVDVVEGIKFINDHGVAYRDLNTQRILLDKNGNACLGDMGILTACRNLGEAMEYETD 480

Query: 481 GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAYSAYSPVQAAVGIAACGLR 540
           GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAY AYSPVQAAVGIAACGLR
Sbjct: 481 GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAYGAYSPVQAAVGIAACGLR 540

Query: 541 PDVPKDCPSILKSLMIKCWNNCPNKRPQFSEILSVLLDNNNNNHR 586
           PDVPKDCPS LK+LMI+CWNNCP+KRPQFSE+LS+LLD+NNNNH+
Sbjct: 541 PDVPKDCPSTLKALMIRCWNNCPSKRPQFSEVLSMLLDSNNNNHK 585

BLAST of Spg001147 vs. NCBI nr
Match: XP_022148438.1 (dual specificity protein kinase shkB isoform X1 [Momordica charantia])

HSP 1 Score: 1117.4 bits (2889), Expect = 0.0e+00
Identity = 552/585 (94.36%), Postives = 565/585 (96.58%), Query Frame = 0

Query: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV 60
           MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV
Sbjct: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV 60

Query: 61  SEAVASIKNSLNLDSARDPSPTKTEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120
           SEAVAS+KNSLNLDSARDPSPTK EGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY
Sbjct: 61  SEAVASLKNSLNLDSARDPSPTKIEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120

Query: 121 DSLPLSYAQAGFDMKDVFLHIKLIEQASVYDHPAILFQEVTNHDVQKPTMKLTFACNSSI 180
           DSLPLSYAQAGF+MKDVFLHIKLIEQASVYDHPAILFQEVTNHD QKPTMK TFACNSSI
Sbjct: 121 DSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNHDTQKPTMKFTFACNSSI 180

Query: 181 SWSTMSGALESADIRCEKIQIFEKKKFTLGVVLFVNLDAQEKVFKSKVENALKLAIKKPK 240
           SWS MSGALESA IRCEKIQIFEKK+FTLGVVLFVN DAQEK FKSK+E+ALKLAIKKPK
Sbjct: 181 SWSAMSGALESAGIRCEKIQIFEKKRFTLGVVLFVNQDAQEKFFKSKIESALKLAIKKPK 240

Query: 241 TNTVKLPFGFCGCQEGNTRGKDLREIEEDAVEQNCRSGFENSNLNENLQIEMPLSTSSFA 300
           TNTVKLPFGFCGCQEGN RGKDLREIEEDAVEQNCRSGF+NSNLNENLQIEMPLSTSSFA
Sbjct: 241 TNTVKLPFGFCGCQEGNARGKDLREIEEDAVEQNCRSGFDNSNLNENLQIEMPLSTSSFA 300

Query: 301 VFVDEWQTVQSGGHELGKWLLSSENLELVDQIGPNSFKGVYKGKRVAIEKIKGCEKGVSY 360
           V VDEWQTVQ GGHELGKWLLSSENLE  DQIGPNSFKGVYKGKRVAIEKIKGCEKG++Y
Sbjct: 301 VSVDEWQTVQCGGHELGKWLLSSENLEFTDQIGPNSFKGVYKGKRVAIEKIKGCEKGIAY 360

Query: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLQTKEI 420
           KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKR QTKEI
Sbjct: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRFQTKEI 420

Query: 421 TRIAIDVVEGIKFMNDHGVAYRDLNTQRILLDKNGNACLGDMGILTACKNLGEAMEYETD 480
           TRI IDV EGIKFMNDHGVAYRDLN QRILLDKNG+ACLGDMGILTACKNLGEAMEYETD
Sbjct: 421 TRIGIDVAEGIKFMNDHGVAYRDLNAQRILLDKNGSACLGDMGILTACKNLGEAMEYETD 480

Query: 481 GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAYSAYSPVQAAVGIAACGLR 540
           GYRWLAPEIIAGDPESVNETWMSN YSFGMVIWEMV+GEAAY AYSPVQAAVGIAACGLR
Sbjct: 481 GYRWLAPEIIAGDPESVNETWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLR 540

Query: 541 PDVPKDCPSILKSLMIKCWNNCPNKRPQFSEILSVLLDNNNNNHR 586
           PD+PKDCPS LKSLMIKCWN CP+KRPQFSEILSVLLD+NNNNHR
Sbjct: 541 PDLPKDCPSTLKSLMIKCWNTCPSKRPQFSEILSVLLDSNNNNHR 585

BLAST of Spg001147 vs. NCBI nr
Match: XP_022986818.1 (fibroblast growth factor receptor [Cucurbita maxima])

HSP 1 Score: 1116.3 bits (2886), Expect = 0.0e+00
Identity = 550/585 (94.02%), Postives = 567/585 (96.92%), Query Frame = 0

Query: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV 60
           M TALECWSSRASTDEDLVEQVLMRTQDRSEGSK ESS AVG KESSAMQKRLQR SRNV
Sbjct: 1   METALECWSSRASTDEDLVEQVLMRTQDRSEGSKSESSLAVGEKESSAMQKRLQRFSRNV 60

Query: 61  SEAVASIKNSLNLDSARDPSPTKTEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120
           SEAVAS+KNSLNLDS RDPSPTKTEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY
Sbjct: 61  SEAVASLKNSLNLDSVRDPSPTKTEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120

Query: 121 DSLPLSYAQAGFDMKDVFLHIKLIEQASVYDHPAILFQEVTNHDVQKPTMKLTFACNSSI 180
           DSLPLSYAQAGFDMKDVFLHIKLIEQASVYDHPAILFQEVTNHDVQKPT+KLTFACNSS+
Sbjct: 121 DSLPLSYAQAGFDMKDVFLHIKLIEQASVYDHPAILFQEVTNHDVQKPTIKLTFACNSSV 180

Query: 181 SWSTMSGALESADIRCEKIQIFEKKKFTLGVVLFVNLDAQEKVFKSKVENALKLAIKKPK 240
           SWS MSGALESA IRCEKIQIFEKKKF+LGV+ FVNLDAQEK+FKSKVENALKLAIKKPK
Sbjct: 181 SWSAMSGALESAGIRCEKIQIFEKKKFSLGVIFFVNLDAQEKLFKSKVENALKLAIKKPK 240

Query: 241 TNTVKLPFGFCGCQEGNTRGKDLREIEEDAVEQNCRSGFENSNLNENLQIEMPLSTSSFA 300
           TNTVKLPFGFCGCQEGNT GKDLREIEEDAV+QNCRSGFENSNLNENLQIEMPLSTSSF 
Sbjct: 241 TNTVKLPFGFCGCQEGNTGGKDLREIEEDAVDQNCRSGFENSNLNENLQIEMPLSTSSFT 300

Query: 301 VFVDEWQTVQSGGHELGKWLLSSENLELVDQIGPNSFKGVYKGKRVAIEKIKGCEKGVSY 360
           V VDEWQTVQSGGHELGKWLLSSENLE  DQIGPNSFKGVYKG+RVAIEKIKGCEKGVSY
Sbjct: 301 VTVDEWQTVQSGGHELGKWLLSSENLEFTDQIGPNSFKGVYKGRRVAIEKIKGCEKGVSY 360

Query: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLQTKEI 420
           KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLQ KEI
Sbjct: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLQMKEI 420

Query: 421 TRIAIDVVEGIKFMNDHGVAYRDLNTQRILLDKNGNACLGDMGILTACKNLGEAMEYETD 480
           TRIA+DVVEGIKF+NDHGVAYRDLNTQRILLDKNGNACLGDMGILTAC+NLGEAMEYETD
Sbjct: 421 TRIAVDVVEGIKFINDHGVAYRDLNTQRILLDKNGNACLGDMGILTACRNLGEAMEYETD 480

Query: 481 GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAYSAYSPVQAAVGIAACGLR 540
           GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAY AYSPVQAAVGIAACGLR
Sbjct: 481 GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAYGAYSPVQAAVGIAACGLR 540

Query: 541 PDVPKDCPSILKSLMIKCWNNCPNKRPQFSEILSVLLDNNNNNHR 586
           PDVPKDCPS LK+LM +CWNNCP+KRPQFSEILS+LLD+NNNNH+
Sbjct: 541 PDVPKDCPSTLKALMTRCWNNCPSKRPQFSEILSMLLDSNNNNHK 585

BLAST of Spg001147 vs. NCBI nr
Match: XP_023512978.1 (mitogen-activated protein kinase kinase kinase 13 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1115.9 bits (2885), Expect = 0.0e+00
Identity = 549/585 (93.85%), Postives = 569/585 (97.26%), Query Frame = 0

Query: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV 60
           MATALECWSSRASTDEDLVEQVLMRTQDRSEGSK ESS AVG KESSAMQKRLQR SRNV
Sbjct: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKTESSLAVGEKESSAMQKRLQRFSRNV 60

Query: 61  SEAVASIKNSLNLDSARDPSPTKTEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120
           SEAVAS+KNSLNLDS RDPSPTKT+GSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY
Sbjct: 61  SEAVASLKNSLNLDSVRDPSPTKTDGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120

Query: 121 DSLPLSYAQAGFDMKDVFLHIKLIEQASVYDHPAILFQEVTNHDVQKPTMKLTFACNSSI 180
           DSLPLSYAQAGF+MKDVFLHIKLIEQASVYDHPAILFQEVTNHDVQKPT+KLTFACNSS+
Sbjct: 121 DSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNHDVQKPTIKLTFACNSSV 180

Query: 181 SWSTMSGALESADIRCEKIQIFEKKKFTLGVVLFVNLDAQEKVFKSKVENALKLAIKKPK 240
           SWS MSGALESA IRCEKIQIFEKKKF+LGV+LFVNLDAQEK+FKSKVENALKLAIKKPK
Sbjct: 181 SWSAMSGALESAGIRCEKIQIFEKKKFSLGVILFVNLDAQEKLFKSKVENALKLAIKKPK 240

Query: 241 TNTVKLPFGFCGCQEGNTRGKDLREIEEDAVEQNCRSGFENSNLNENLQIEMPLSTSSFA 300
           TNTVKLPFGFCGCQEGNT GKDLREIEEDAV+QNCRSGFEN+NLNENLQIEMPLSTSSF 
Sbjct: 241 TNTVKLPFGFCGCQEGNTGGKDLREIEEDAVDQNCRSGFENTNLNENLQIEMPLSTSSFT 300

Query: 301 VFVDEWQTVQSGGHELGKWLLSSENLELVDQIGPNSFKGVYKGKRVAIEKIKGCEKGVSY 360
           V VDEWQTVQSGGHELGKWLLSSENLE  DQIGPNSFKGVYKG+RVAIEKIKGCEKGVSY
Sbjct: 301 VTVDEWQTVQSGGHELGKWLLSSENLEFTDQIGPNSFKGVYKGRRVAIEKIKGCEKGVSY 360

Query: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLQTKEI 420
           KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRL  KEI
Sbjct: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLHMKEI 420

Query: 421 TRIAIDVVEGIKFMNDHGVAYRDLNTQRILLDKNGNACLGDMGILTACKNLGEAMEYETD 480
           TRIA+DVVEGIKF+NDHGVAYRDLNTQRILLDKNGNACLGDMGILTAC+NLGEAMEYETD
Sbjct: 421 TRIAVDVVEGIKFINDHGVAYRDLNTQRILLDKNGNACLGDMGILTACRNLGEAMEYETD 480

Query: 481 GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAYSAYSPVQAAVGIAACGLR 540
           GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAY AYSPVQAAVGIAACGLR
Sbjct: 481 GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAYGAYSPVQAAVGIAACGLR 540

Query: 541 PDVPKDCPSILKSLMIKCWNNCPNKRPQFSEILSVLLDNNNNNHR 586
           PDVPKDCPS LK+LMI+CWNNCP+KRPQFSEILS+LLD+NNNNH+
Sbjct: 541 PDVPKDCPSTLKALMIRCWNNCPSKRPQFSEILSMLLDSNNNNHK 585

BLAST of Spg001147 vs. NCBI nr
Match: XP_038902013.1 (serine/threonine-protein kinase EDR1 [Benincasa hispida])

HSP 1 Score: 1114.4 bits (2881), Expect = 0.0e+00
Identity = 551/585 (94.19%), Postives = 567/585 (96.92%), Query Frame = 0

Query: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV 60
           MATALECWSSRASTDED+VEQVLMRTQDRSE SK ESSFAVG KESS M KRLQR SRNV
Sbjct: 1   MATALECWSSRASTDEDMVEQVLMRTQDRSEDSKAESSFAVGEKESSTMHKRLQRFSRNV 60

Query: 61  SEAVASIKNSLNLDSARDPSPTKTEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120
           SEAVAS+KNSLNLDS RDPSPT+TEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY
Sbjct: 61  SEAVASLKNSLNLDSVRDPSPTRTEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120

Query: 121 DSLPLSYAQAGFDMKDVFLHIKLIEQASVYDHPAILFQEVTNHDVQKPTMKLTFACNSSI 180
           DSLPLSYAQAGFDMKDVFLHIKLIEQASVYDHPAILFQEVTN DVQKP MKLTFACNSS+
Sbjct: 121 DSLPLSYAQAGFDMKDVFLHIKLIEQASVYDHPAILFQEVTNRDVQKPIMKLTFACNSSV 180

Query: 181 SWSTMSGALESADIRCEKIQIFEKKKFTLGVVLFVNLDAQEKVFKSKVENALKLAIKKPK 240
           SWS MSGALESA IRCEKIQIFEKKKFTLGV+LFVNLD QEK+FKSKVENALKLAIKKPK
Sbjct: 181 SWSAMSGALESAAIRCEKIQIFEKKKFTLGVILFVNLDVQEKLFKSKVENALKLAIKKPK 240

Query: 241 TNTVKLPFGFCGCQEGNTRGKDLREIEEDAVEQNCRSGFENSNLNENLQIEMPLSTSSFA 300
           T TVKLPFGFCGCQEGNT GKDLREIE+DAVEQNCRSG+ENSNL++NL+IEMPLSTSSFA
Sbjct: 241 TTTVKLPFGFCGCQEGNTGGKDLREIEDDAVEQNCRSGYENSNLSDNLRIEMPLSTSSFA 300

Query: 301 VFVDEWQTVQSGGHELGKWLLSSENLELVDQIGPNSFKGVYKGKRVAIEKIKGCEKGVSY 360
           V VDEWQTVQSGGHELGKWLLSSENLE VDQIGPNSFKGVYKG+RVAIEKIKGCEKGV+Y
Sbjct: 301 VTVDEWQTVQSGGHELGKWLLSSENLEFVDQIGPNSFKGVYKGRRVAIEKIKGCEKGVAY 360

Query: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLQTKEI 420
           KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLQTKEI
Sbjct: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLQTKEI 420

Query: 421 TRIAIDVVEGIKFMNDHGVAYRDLNTQRILLDKNGNACLGDMGILTACKNLGEAMEYETD 480
           TRIAIDVVEGIKFMNDHGVAYRDLNTQRILLDKNGNACLGDMGILTACKNLGEAMEYETD
Sbjct: 421 TRIAIDVVEGIKFMNDHGVAYRDLNTQRILLDKNGNACLGDMGILTACKNLGEAMEYETD 480

Query: 481 GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAYSAYSPVQAAVGIAACGLR 540
           GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAY AYSPVQAAVGIAACGLR
Sbjct: 481 GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAYGAYSPVQAAVGIAACGLR 540

Query: 541 PDVPKDCPSILKSLMIKCWNNCPNKRPQFSEILSVLLDNNNNNHR 586
           PDVPKDCPS LKSLMI+CWNNCP+KRPQFSEILSVLLD+NNNNHR
Sbjct: 541 PDVPKDCPSTLKSLMIRCWNNCPSKRPQFSEILSVLLDSNNNNHR 585

BLAST of Spg001147 vs. ExPASy Swiss-Prot
Match: Q8RWL6 (Serine/threonine-protein kinase STY17 OS=Arabidopsis thaliana OX=3702 GN=STY17 PE=1 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 1.1e-38
Identity = 84/264 (31.82%), Postives = 149/264 (56.44%), Query Frame = 0

Query: 318 KWLLSSENLELVDQIGPNS----FKGVYKGKRVAIEKIKGCEKGVSYKFELRKDLLELMT 377
           +W +  + L++  ++   S    F+G Y  + VAI+ +K          E  +++  +  
Sbjct: 284 EWEIDMKQLKIEKKVACGSYGELFRGTYCSQEVAIKILKPERVNAEMLREFSQEVYIMRK 343

Query: 378 CGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRL-QTKEITRIAIDVVEGIK 437
             HKN++ F G C   +  LC+VT+ M  GS+++ + K+K + + + + ++A+DV +G+ 
Sbjct: 344 VRHKNVVQFIGAC-TRSPNLCIVTEFMTRGSIYDFLHKHKGVFKIQSLLKVALDVSKGMN 403

Query: 438 FMNDHGVAYRDLNTQRILLDKNGNACLGDMGILTACKNLGEAMEYETDGYRWLAPEIIAG 497
           +++ + + +RDL T  +L+D++    + D G+       G  M  ET  YRW+APE+I  
Sbjct: 404 YLHQNNIIHRDLKTANLLMDEHEVVKVADFGVARVQTESG-VMTAETGTYRWMAPEVIEH 463

Query: 498 DPESVNETWMSNVYSFGMVIWEMVTGEAAYSAYSPVQAAVGIAACGLRPDVPKDCPSILK 557
            P        ++V+S+ +V+WE++TGE  YS  +P+QAAVG+   GLRP +PK+    L 
Sbjct: 464 KPYD----HRADVFSYAIVLWELLTGELPYSYLTPLQAAVGVVQKGLRPKIPKETHPKLT 523

Query: 558 SLMIKCWNNCPNKRPQFSEILSVL 577
            L+ KCW   P  RP F+EI+ +L
Sbjct: 524 ELLEKCWQQDPALRPNFAEIIEML 541

BLAST of Spg001147 vs. ExPASy Swiss-Prot
Match: O22558 (Serine/threonine-protein kinase STY8 OS=Arabidopsis thaliana OX=3702 GN=STY8 PE=1 SV=2)

HSP 1 Score: 161.4 bits (407), Expect = 3.1e-38
Identity = 84/266 (31.58%), Postives = 151/266 (56.77%), Query Frame = 0

Query: 318 KWLLSSENLELVDQIGPNSF----KGVYKGKRVAIEKIKGCEKGVSYKFELRKDLLELMT 377
           +W +    L++  ++   S+    +G Y  + VAI+ +K          E  +++  +  
Sbjct: 278 EWEIDVTQLKIEKKVASGSYGDLHRGTYCSQEVAIKFLKPDRVNNEMLREFSQEVFIMRK 337

Query: 378 CGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNK---RLQTKEITRIAIDVVEG 437
             HKN++ F G C   +  LC+VT+ M  GS+++ + K K   +LQT  + ++A+DV +G
Sbjct: 338 VRHKNVVQFLGAC-TRSPTLCIVTEFMARGSIYDFLHKQKCAFKLQT--LLKVALDVAKG 397

Query: 438 IKFMNDHGVAYRDLNTQRILLDKNGNACLGDMGILTACKNLGEAMEYETDGYRWLAPEII 497
           + +++ + + +RDL T  +L+D++G   + D G+       G  M  ET  YRW+APE+I
Sbjct: 398 MSYLHQNNIIHRDLKTANLLMDEHGLVKVADFGVARVQIESG-VMTAETGTYRWMAPEVI 457

Query: 498 AGDPESVNETWMSNVYSFGMVIWEMVTGEAAYSAYSPVQAAVGIAACGLRPDVPKDCPSI 557
              P +      ++V+S+ +V+WE++TG+  Y+  +P+QAAVG+   GLRP +PK     
Sbjct: 458 EHKPYN----HKADVFSYAIVLWELLTGDIPYAFLTPLQAAVGVVQKGLRPKIPKKTHPK 517

Query: 558 LKSLMIKCWNNCPNKRPQFSEILSVL 577
           +K L+ +CW+  P +RP F EI+ +L
Sbjct: 518 VKGLLERCWHQDPEQRPLFEEIIEML 535

BLAST of Spg001147 vs. ExPASy Swiss-Prot
Match: F4JTP5 (Serine/threonine-protein kinase STY46 OS=Arabidopsis thaliana OX=3702 GN=STY46 PE=1 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 4.1e-38
Identity = 85/263 (32.32%), Postives = 148/263 (56.27%), Query Frame = 0

Query: 319 WLLSSENLELVDQIGPNSFKGVYKG----KRVAIEKIKGCEKGVSYKFELRKDLLELMTC 378
           W ++ ++L+   +I   S+  +YKG    + VAI+ +K        + E  +++  +   
Sbjct: 283 WEINLKHLKFGHKIASGSYGDLYKGTYCSQEVAIKVLKPERLDSDLEKEFAQEVFIMRKV 342

Query: 379 GHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRL-QTKEITRIAIDVVEGIKF 438
            HKN++ F G C    H LC+VT+ M GGSV++ + K K + +   + ++AID+ +G+ +
Sbjct: 343 RHKNVVQFIGACTKPPH-LCIVTEFMPGGSVYDYLHKQKGVFKLPTLFKVAIDICKGMSY 402

Query: 439 MNDHGVAYRDLNTQRILLDKNGNACLGDMGILTACKNLGEAMEYETDGYRWLAPEIIAGD 498
           ++ + + +RDL    +L+D+N    + D G+       G  M  ET  YRW+APE+I   
Sbjct: 403 LHQNNIIHRDLKAANLLMDENEVVKVADFGVARVKAQTG-VMTAETGTYRWMAPEVIEHK 462

Query: 499 PESVNETWMSNVYSFGMVIWEMVTGEAAYSAYSPVQAAVGIAACGLRPDVPKDCPSILKS 558
           P        ++V+S+G+V+WE++TG+  Y   +P+QAAVG+   GLRP +PK+    L  
Sbjct: 463 PYD----HKADVFSYGIVLWELLTGKLPYEYMTPLQAAVGVVQKGLRPTIPKNTHPKLAE 522

Query: 559 LMIKCWNNCPNKRPQFSEILSVL 577
           L+ + W +   +RP FSEI+  L
Sbjct: 523 LLERLWEHDSTQRPDFSEIIEQL 539

BLAST of Spg001147 vs. ExPASy Swiss-Prot
Match: Q55GU0 (Probable serine/threonine-protein kinase DDB_G0267514 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0267514 PE=3 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 2.0e-32
Identity = 82/262 (31.30%), Postives = 145/262 (55.34%), Query Frame = 0

Query: 321 LSSENLELVDQIGPNSF----KGVYKGKRVAIEKIKGCEKGVSYKF-ELRKDLLELMTCG 380
           +S   L++  ++G  +F    KG+++G  VAI++IK  E   +    E RK+L  L    
Sbjct: 657 ISFSELKISSKLGEGTFGVVYKGLWRGSSVAIKQIKINEDVNNQVLEEFRKELTILSRLR 716

Query: 381 HKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNK-RLQTKEITRIAIDVVEGIKFM 440
           H NI++    C    + LC +T+ + GGS+++ +   K ++  +   ++AI + +G+ ++
Sbjct: 717 HPNIVLLMAACTAPPN-LCFITEYLPGGSLYDALHSKKIKMNMQLYKKLAIQIAQGMNYL 776

Query: 441 NDHGVAYRDLNTQRILLDKNGNACLGDMGILTACKNLGEAMEYETDGYRWLAPEIIAGDP 500
           +  GV +RD+ +  +LLD++ N  + D G L+  K+    M        W++PE++ G+ 
Sbjct: 777 HLSGVIHRDIKSLNLLLDEHMNVKICDFG-LSKLKSKSTEMTKSIGSPIWMSPELLMGE- 836

Query: 501 ESVNETWMSNVYSFGMVIWEMVTGEAAYSAYSPVQAAVGIAACGLRPDVPKDCPSILKSL 560
              + T   +VY+FG+++WE+ TGE  YS    VQ A+ +    LRP +P   P  L  L
Sbjct: 837 ---DYTEKVDVYAFGIILWELGTGELPYSGLDSVQLALAVTTKSLRPPIPNAWPYQLSHL 896

Query: 561 MIKCWNNCPNKRPQFSEILSVL 577
           +  CW+  P KRP F+EIL++L
Sbjct: 897 IQACWHQDPLKRPSFTEILNLL 912

BLAST of Spg001147 vs. ExPASy Swiss-Prot
Match: Q7TQP6 (Serine/threonine-protein kinase TNNI3K OS=Rattus norvegicus OX=10116 GN=Tnni3k PE=2 SV=3)

HSP 1 Score: 141.7 bits (356), Expect = 2.6e-32
Identity = 88/269 (32.71%), Postives = 146/269 (54.28%), Query Frame = 0

Query: 321 LSSENLELVDQIGPNSFKGVYKG----KRVAIEKIKG---CEKGVSYKFELRKDLLELMT 380
           L    +E  + IG  SF  VYKG    K VAI++ +    C K     F   +++  L  
Sbjct: 458 LQLSEIEFHEIIGSGSFGKVYKGRCRNKIVAIKRYRANTYCSKSDVDMF--CREVSILCQ 517

Query: 381 CGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKR---LQTKEITRIAIDVVEG 440
             H  ++ F G C+D+     +VT+ + GGS+  L+ + KR   LQ+K I  IA+DV +G
Sbjct: 518 LNHPCVVQFVGACLDDPSQFAIVTQYISGGSLFSLLHEQKRILDLQSKLI--IAVDVAKG 577

Query: 441 IKFMND--HGVAYRDLNTQRILLDKNGNACLGDMGILTACKNLGE-AMEYETDGYRWLAP 500
           +++++     + +RDLN+  ILL ++G+A + D G     ++L E  M  +    RW+AP
Sbjct: 578 MEYLHSLTQPIIHRDLNSHNILLYEDGHAVVADFGESRFLQSLDEDNMTKQPGNLRWMAP 637

Query: 501 EIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAYSAYSPVQAAVGIAACGLRPDVPKDC 560
           E+     +    T  ++V+S+ + +WE++TGE  ++   P  AA  +A   +RP +    
Sbjct: 638 EVFT---QCTRYTIKADVFSYSLCLWELLTGEIPFAHLKPAAAAADMAYHHIRPPIGYSI 697

Query: 561 PSILKSLMIKCWNNCPNKRPQFSEILSVL 577
           P  + SL+I+ WN CP  RP+FSE++S L
Sbjct: 698 PKPISSLLIRGWNACPEGRPEFSEVVSKL 719

BLAST of Spg001147 vs. ExPASy TrEMBL
Match: A0A6J1D436 (dual specificity protein kinase shkB isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017085 PE=4 SV=1)

HSP 1 Score: 1117.4 bits (2889), Expect = 0.0e+00
Identity = 552/585 (94.36%), Postives = 565/585 (96.58%), Query Frame = 0

Query: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV 60
           MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV
Sbjct: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV 60

Query: 61  SEAVASIKNSLNLDSARDPSPTKTEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120
           SEAVAS+KNSLNLDSARDPSPTK EGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY
Sbjct: 61  SEAVASLKNSLNLDSARDPSPTKIEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120

Query: 121 DSLPLSYAQAGFDMKDVFLHIKLIEQASVYDHPAILFQEVTNHDVQKPTMKLTFACNSSI 180
           DSLPLSYAQAGF+MKDVFLHIKLIEQASVYDHPAILFQEVTNHD QKPTMK TFACNSSI
Sbjct: 121 DSLPLSYAQAGFEMKDVFLHIKLIEQASVYDHPAILFQEVTNHDTQKPTMKFTFACNSSI 180

Query: 181 SWSTMSGALESADIRCEKIQIFEKKKFTLGVVLFVNLDAQEKVFKSKVENALKLAIKKPK 240
           SWS MSGALESA IRCEKIQIFEKK+FTLGVVLFVN DAQEK FKSK+E+ALKLAIKKPK
Sbjct: 181 SWSAMSGALESAGIRCEKIQIFEKKRFTLGVVLFVNQDAQEKFFKSKIESALKLAIKKPK 240

Query: 241 TNTVKLPFGFCGCQEGNTRGKDLREIEEDAVEQNCRSGFENSNLNENLQIEMPLSTSSFA 300
           TNTVKLPFGFCGCQEGN RGKDLREIEEDAVEQNCRSGF+NSNLNENLQIEMPLSTSSFA
Sbjct: 241 TNTVKLPFGFCGCQEGNARGKDLREIEEDAVEQNCRSGFDNSNLNENLQIEMPLSTSSFA 300

Query: 301 VFVDEWQTVQSGGHELGKWLLSSENLELVDQIGPNSFKGVYKGKRVAIEKIKGCEKGVSY 360
           V VDEWQTVQ GGHELGKWLLSSENLE  DQIGPNSFKGVYKGKRVAIEKIKGCEKG++Y
Sbjct: 301 VSVDEWQTVQCGGHELGKWLLSSENLEFTDQIGPNSFKGVYKGKRVAIEKIKGCEKGIAY 360

Query: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLQTKEI 420
           KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKR QTKEI
Sbjct: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRFQTKEI 420

Query: 421 TRIAIDVVEGIKFMNDHGVAYRDLNTQRILLDKNGNACLGDMGILTACKNLGEAMEYETD 480
           TRI IDV EGIKFMNDHGVAYRDLN QRILLDKNG+ACLGDMGILTACKNLGEAMEYETD
Sbjct: 421 TRIGIDVAEGIKFMNDHGVAYRDLNAQRILLDKNGSACLGDMGILTACKNLGEAMEYETD 480

Query: 481 GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAYSAYSPVQAAVGIAACGLR 540
           GYRWLAPEIIAGDPESVNETWMSN YSFGMVIWEMV+GEAAY AYSPVQAAVGIAACGLR
Sbjct: 481 GYRWLAPEIIAGDPESVNETWMSNAYSFGMVIWEMVSGEAAYGAYSPVQAAVGIAACGLR 540

Query: 541 PDVPKDCPSILKSLMIKCWNNCPNKRPQFSEILSVLLDNNNNNHR 586
           PD+PKDCPS LKSLMIKCWN CP+KRPQFSEILSVLLD+NNNNHR
Sbjct: 541 PDLPKDCPSTLKSLMIKCWNTCPSKRPQFSEILSVLLDSNNNNHR 585

BLAST of Spg001147 vs. ExPASy TrEMBL
Match: A0A6J1JHM8 (fibroblast growth factor receptor OS=Cucurbita maxima OX=3661 GN=LOC111484449 PE=4 SV=1)

HSP 1 Score: 1116.3 bits (2886), Expect = 0.0e+00
Identity = 550/585 (94.02%), Postives = 567/585 (96.92%), Query Frame = 0

Query: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV 60
           M TALECWSSRASTDEDLVEQVLMRTQDRSEGSK ESS AVG KESSAMQKRLQR SRNV
Sbjct: 1   METALECWSSRASTDEDLVEQVLMRTQDRSEGSKSESSLAVGEKESSAMQKRLQRFSRNV 60

Query: 61  SEAVASIKNSLNLDSARDPSPTKTEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120
           SEAVAS+KNSLNLDS RDPSPTKTEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY
Sbjct: 61  SEAVASLKNSLNLDSVRDPSPTKTEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120

Query: 121 DSLPLSYAQAGFDMKDVFLHIKLIEQASVYDHPAILFQEVTNHDVQKPTMKLTFACNSSI 180
           DSLPLSYAQAGFDMKDVFLHIKLIEQASVYDHPAILFQEVTNHDVQKPT+KLTFACNSS+
Sbjct: 121 DSLPLSYAQAGFDMKDVFLHIKLIEQASVYDHPAILFQEVTNHDVQKPTIKLTFACNSSV 180

Query: 181 SWSTMSGALESADIRCEKIQIFEKKKFTLGVVLFVNLDAQEKVFKSKVENALKLAIKKPK 240
           SWS MSGALESA IRCEKIQIFEKKKF+LGV+ FVNLDAQEK+FKSKVENALKLAIKKPK
Sbjct: 181 SWSAMSGALESAGIRCEKIQIFEKKKFSLGVIFFVNLDAQEKLFKSKVENALKLAIKKPK 240

Query: 241 TNTVKLPFGFCGCQEGNTRGKDLREIEEDAVEQNCRSGFENSNLNENLQIEMPLSTSSFA 300
           TNTVKLPFGFCGCQEGNT GKDLREIEEDAV+QNCRSGFENSNLNENLQIEMPLSTSSF 
Sbjct: 241 TNTVKLPFGFCGCQEGNTGGKDLREIEEDAVDQNCRSGFENSNLNENLQIEMPLSTSSFT 300

Query: 301 VFVDEWQTVQSGGHELGKWLLSSENLELVDQIGPNSFKGVYKGKRVAIEKIKGCEKGVSY 360
           V VDEWQTVQSGGHELGKWLLSSENLE  DQIGPNSFKGVYKG+RVAIEKIKGCEKGVSY
Sbjct: 301 VTVDEWQTVQSGGHELGKWLLSSENLEFTDQIGPNSFKGVYKGRRVAIEKIKGCEKGVSY 360

Query: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLQTKEI 420
           KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLQ KEI
Sbjct: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLQMKEI 420

Query: 421 TRIAIDVVEGIKFMNDHGVAYRDLNTQRILLDKNGNACLGDMGILTACKNLGEAMEYETD 480
           TRIA+DVVEGIKF+NDHGVAYRDLNTQRILLDKNGNACLGDMGILTAC+NLGEAMEYETD
Sbjct: 421 TRIAVDVVEGIKFINDHGVAYRDLNTQRILLDKNGNACLGDMGILTACRNLGEAMEYETD 480

Query: 481 GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAYSAYSPVQAAVGIAACGLR 540
           GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAY AYSPVQAAVGIAACGLR
Sbjct: 481 GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAYGAYSPVQAAVGIAACGLR 540

Query: 541 PDVPKDCPSILKSLMIKCWNNCPNKRPQFSEILSVLLDNNNNNHR 586
           PDVPKDCPS LK+LM +CWNNCP+KRPQFSEILS+LLD+NNNNH+
Sbjct: 541 PDVPKDCPSTLKALMTRCWNNCPSKRPQFSEILSMLLDSNNNNHK 585

BLAST of Spg001147 vs. ExPASy TrEMBL
Match: A0A6J1FY74 (mitogen-activated protein kinase kinase kinase 13 OS=Cucurbita moschata OX=3662 GN=LOC111449006 PE=4 SV=1)

HSP 1 Score: 1110.9 bits (2872), Expect = 0.0e+00
Identity = 547/585 (93.50%), Postives = 568/585 (97.09%), Query Frame = 0

Query: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV 60
           MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESS AVG KESSAMQKRLQR SRNV
Sbjct: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSLAVGEKESSAMQKRLQRFSRNV 60

Query: 61  SEAVASIKNSLNLDSARDPSPTKTEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120
           SEAVAS+KNSLNLDS RDPSPTKTEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY
Sbjct: 61  SEAVASLKNSLNLDSVRDPSPTKTEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120

Query: 121 DSLPLSYAQAGFDMKDVFLHIKLIEQASVYDHPAILFQEVTNHDVQKPTMKLTFACNSSI 180
           DSLPLSYAQAGF++KDVFLHIKLIEQASVYDHPAILFQEVTNHDVQKPT+KLTFACNS +
Sbjct: 121 DSLPLSYAQAGFEIKDVFLHIKLIEQASVYDHPAILFQEVTNHDVQKPTIKLTFACNSLV 180

Query: 181 SWSTMSGALESADIRCEKIQIFEKKKFTLGVVLFVNLDAQEKVFKSKVENALKLAIKKPK 240
           SWS MSGALESA IRCEKIQIFEKKKF+LGV+LFVNLDAQEK+FKSKVENALKLAIKKPK
Sbjct: 181 SWSAMSGALESAGIRCEKIQIFEKKKFSLGVILFVNLDAQEKLFKSKVENALKLAIKKPK 240

Query: 241 TNTVKLPFGFCGCQEGNTRGKDLREIEEDAVEQNCRSGFENSNLNENLQIEMPLSTSSFA 300
           TNTVKLPFGFCGCQEGNT GKDLREIEEDAV+QNCRSGFENSNLNENL IEMPLSTSSF 
Sbjct: 241 TNTVKLPFGFCGCQEGNTGGKDLREIEEDAVDQNCRSGFENSNLNENLLIEMPLSTSSFT 300

Query: 301 VFVDEWQTVQSGGHELGKWLLSSENLELVDQIGPNSFKGVYKGKRVAIEKIKGCEKGVSY 360
           V VDEWQTVQSGGHELGKWLLSSENLE  DQIGPNSFKGVYKG+RVAIEKIKGCEKGVSY
Sbjct: 301 VTVDEWQTVQSGGHELGKWLLSSENLEFTDQIGPNSFKGVYKGRRVAIEKIKGCEKGVSY 360

Query: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLQTKEI 420
           KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLQ KEI
Sbjct: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLQMKEI 420

Query: 421 TRIAIDVVEGIKFMNDHGVAYRDLNTQRILLDKNGNACLGDMGILTACKNLGEAMEYETD 480
           TRIA DVVEGIKF+NDHGVAYRDLNTQRILLDKNGNACLGDMGILTAC+NLGEAMEYETD
Sbjct: 421 TRIAADVVEGIKFINDHGVAYRDLNTQRILLDKNGNACLGDMGILTACRNLGEAMEYETD 480

Query: 481 GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAYSAYSPVQAAVGIAACGLR 540
           GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAY AYSPVQAAVGIAACGLR
Sbjct: 481 GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAYGAYSPVQAAVGIAACGLR 540

Query: 541 PDVPKDCPSILKSLMIKCWNNCPNKRPQFSEILSVLLDNNNNNHR 586
           PDVPKDCPS LK+LMI+CWNNCP+KRPQFSE+LS++LD+NNN+H+
Sbjct: 541 PDVPKDCPSNLKALMIRCWNNCPSKRPQFSEVLSMVLDSNNNSHK 585

BLAST of Spg001147 vs. ExPASy TrEMBL
Match: A0A0A0KC34 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G181580 PE=4 SV=1)

HSP 1 Score: 1093.6 bits (2827), Expect = 0.0e+00
Identity = 538/585 (91.97%), Postives = 561/585 (95.90%), Query Frame = 0

Query: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV 60
           MATALECWSSRASTDED+VEQVLMRTQDRSEGSKP+SSFAVG KESSAM +RLQR SRNV
Sbjct: 1   MATALECWSSRASTDEDMVEQVLMRTQDRSEGSKPDSSFAVGEKESSAMHRRLQRFSRNV 60

Query: 61  SEAVASIKNSLNLDSARDPSPTKTEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120
           SEAVAS+KNSLNLDS RDPSPT+TEGSKKAVWG+VVRNLTQLYPGSQLPEKLVSNIRKHY
Sbjct: 61  SEAVASLKNSLNLDSIRDPSPTRTEGSKKAVWGTVVRNLTQLYPGSQLPEKLVSNIRKHY 120

Query: 121 DSLPLSYAQAGFDMKDVFLHIKLIEQASVYDHPAILFQEVTNHDVQKPTMKLTFACNSSI 180
           DSLPLSYA AGFDMKDVFLHIKL+EQASVYDHPAILFQEVTN DVQKP +K TFACNSS+
Sbjct: 121 DSLPLSYALAGFDMKDVFLHIKLMEQASVYDHPAILFQEVTNRDVQKPIIKFTFACNSSV 180

Query: 181 SWSTMSGALESADIRCEKIQIFEKKKFTLGVVLFVNLDAQEKVFKSKVENALKLAIKKPK 240
           SWS MSGALE+A IRCEKIQIFEKKKFTLGV+LFVNLD QEK+FKSKVENALKLAIKKPK
Sbjct: 181 SWSAMSGALETAAIRCEKIQIFEKKKFTLGVILFVNLDLQEKLFKSKVENALKLAIKKPK 240

Query: 241 TNTVKLPFGFCGCQEGNTRGKDLREIEEDAVEQNCRSGFENSNLNENLQIEMPLSTSSFA 300
           T  VKLPFGFCGCQEGNT GKDLRE EED VE NCRSGFENSNL+ENLQIEMPL TSSFA
Sbjct: 241 TTAVKLPFGFCGCQEGNTGGKDLRETEEDGVEPNCRSGFENSNLSENLQIEMPLCTSSFA 300

Query: 301 VFVDEWQTVQSGGHELGKWLLSSENLELVDQIGPNSFKGVYKGKRVAIEKIKGCEKGVSY 360
           V VDEWQT+QSGG+ELGKWLLSSENLE VDQ+GPNSFKGVYKG+RVAIEKIKGCEKGVSY
Sbjct: 301 VTVDEWQTIQSGGNELGKWLLSSENLEFVDQMGPNSFKGVYKGRRVAIEKIKGCEKGVSY 360

Query: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLQTKEI 420
           KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNK+LQTKEI
Sbjct: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKKLQTKEI 420

Query: 421 TRIAIDVVEGIKFMNDHGVAYRDLNTQRILLDKNGNACLGDMGILTACKNLGEAMEYETD 480
           TRIAID+VEGIKFMNDHGVAYRDLNTQRILLDKNGNACLGDMGILTACKNLGEAMEYETD
Sbjct: 421 TRIAIDIVEGIKFMNDHGVAYRDLNTQRILLDKNGNACLGDMGILTACKNLGEAMEYETD 480

Query: 481 GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAYSAYSPVQAAVGIAACGLR 540
           GYRWLAPEIIAGDPESVNETWMSNVYS GMVIWEMVTGEAAY AYSPVQAAVGIAACGLR
Sbjct: 481 GYRWLAPEIIAGDPESVNETWMSNVYSLGMVIWEMVTGEAAYGAYSPVQAAVGIAACGLR 540

Query: 541 PDVPKDCPSILKSLMIKCWNNCPNKRPQFSEILSVLLDNNNNNHR 586
           PDVPKDC S LKSLMI+CWNNCP+KRPQFSEILS+LLD+NNNNHR
Sbjct: 541 PDVPKDCSSTLKSLMIRCWNNCPSKRPQFSEILSLLLDSNNNNHR 585

BLAST of Spg001147 vs. ExPASy TrEMBL
Match: A0A6J1L4L5 (serine/threonine-protein kinase EDR1-like OS=Cucurbita maxima OX=3661 GN=LOC111499850 PE=4 SV=1)

HSP 1 Score: 1090.9 bits (2820), Expect = 0.0e+00
Identity = 540/585 (92.31%), Postives = 559/585 (95.56%), Query Frame = 0

Query: 1   MATALECWSSRASTDEDLVEQVLMRTQDRSEGSKPESSFAVGAKESSAMQKRLQRLSRNV 60
           MATALECWSSRASTDED+VEQVLMRTQDRSEGSK ESSF VG KESSAMQKRLQRLSRNV
Sbjct: 1   MATALECWSSRASTDEDVVEQVLMRTQDRSEGSKAESSFGVGVKESSAMQKRLQRLSRNV 60

Query: 61  SEAVASIKNSLNLDSARDPSPTKTEGSKKAVWGSVVRNLTQLYPGSQLPEKLVSNIRKHY 120
           SEAVASIKNSLNLDS RDPSP++TEGSKK VWGSVVRNLT LYPGSQLPEKLVS+IRKHY
Sbjct: 61  SEAVASIKNSLNLDSVRDPSPSRTEGSKKEVWGSVVRNLTLLYPGSQLPEKLVSSIRKHY 120

Query: 121 DSLPLSYAQAGFDMKDVFLHIKLIEQASVYDHPAILFQEVTNHDVQKPTMKLTFACNSSI 180
           DSLP SYAQAGFDMKDVFLHIKLIEQASVY+HPAI FQEVT +DVQKPTMKLTFACNSSI
Sbjct: 121 DSLPFSYAQAGFDMKDVFLHIKLIEQASVYEHPAIFFQEVTYNDVQKPTMKLTFACNSSI 180

Query: 181 SWSTMSGALESADIRCEKIQIFEKKKFTLGVVLFVNLDAQEKVFKSKVENALKLAIKKPK 240
           SWS MSGALE +DIRCEKIQIFEKKKFTLGV+LF NLDAQE++FKSKVENALKLAIKKPK
Sbjct: 181 SWSAMSGALEGSDIRCEKIQIFEKKKFTLGVILFANLDAQEELFKSKVENALKLAIKKPK 240

Query: 241 TNTVKLPFGFCGCQEGNTRGKDLREIEEDAVEQNCRSGFENSNLNENLQIEMPLSTSSFA 300
           TNTVKLPFGFCG Q+GNTRGKDLREIEEDA+EQNCRSGFE  N NENLQIEMPLSTSSFA
Sbjct: 241 TNTVKLPFGFCGGQDGNTRGKDLREIEEDAIEQNCRSGFERLNSNENLQIEMPLSTSSFA 300

Query: 301 VFVDEWQTVQSGGHELGKWLLSSENLELVDQIGPNSFKGVYKGKRVAIEKIKGCEKGVSY 360
           V VDEWQTVQSGGHELGKWLLSSENLE  DQIGPNSFKGVYKGKRV IEKIKGCEKGVSY
Sbjct: 301 VSVDEWQTVQSGGHELGKWLLSSENLEFSDQIGPNSFKGVYKGKRVCIEKIKGCEKGVSY 360

Query: 361 KFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLQTKEI 420
           KFELRKDLLELMT GHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLQTKEI
Sbjct: 361 KFELRKDLLELMTYGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRLQTKEI 420

Query: 421 TRIAIDVVEGIKFMNDHGVAYRDLNTQRILLDKNGNACLGDMGILTACKNLGEAMEYETD 480
           TRIAIDV EG+KFMNDHG+AYRDLNTQRIL+DKNGNACLGDMGIL ACKNLGEAMEYETD
Sbjct: 421 TRIAIDVAEGMKFMNDHGIAYRDLNTQRILIDKNGNACLGDMGILIACKNLGEAMEYETD 480

Query: 481 GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAYSAYSPVQAAVGIAACGLR 540
           GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAY AYSPVQAAVGIAACGLR
Sbjct: 481 GYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAYGAYSPVQAAVGIAACGLR 540

Query: 541 PDVPKDCPSILKSLMIKCWNNCPNKRPQFSEILSVLLDNNNNNHR 586
           PDVPKDCP+ LKSLMI+CWNNCP+KRPQFSEILSVLLD+NNN HR
Sbjct: 541 PDVPKDCPTTLKSLMIRCWNNCPSKRPQFSEILSVLLDSNNNKHR 585

BLAST of Spg001147 vs. TAIR 10
Match: AT5G58520.1 (Protein kinase superfamily protein )

HSP 1 Score: 810.4 bits (2092), Expect = 9.1e-235
Identity = 401/601 (66.72%), Postives = 483/601 (80.37%), Query Frame = 0

Query: 1   MATALECWSSRA----STDEDLVEQVLMRTQDRSEG---SKPESSFAVGA------KESS 60
           MA ALECWSSRA      D DLV+QVLMRT DRSE    S PE+S  V        + SS
Sbjct: 1   MAAALECWSSRAGDGGDPDNDLVDQVLMRTHDRSESVITSLPETSLEVEGSTTVFDQSSS 60

Query: 61  AMQKRLQRLSRNVSEAVASIKNSLNLDSARD------PSPTKTE-----GSKKAVWGSVV 120
           AMQKR QRLSRNVSEA+ S+KN+LNLDSARD          K E     G +K VW +VV
Sbjct: 61  AMQKRFQRLSRNVSEAIVSLKNTLNLDSARDNQSFGGAMTPKAEVSGGGGGRKLVWATVV 120

Query: 121 RNLTQLYPGSQLPEKLVSNIRKHYDSLPLSYAQAGFDMKDVFLHIKLIEQASVYDHPAIL 180
           +NL ++YPGSQLPEKLVSN++KHYDSLP SY+QA FDMK+VFLH+KLIEQA+  D+P  +
Sbjct: 121 KNLAKMYPGSQLPEKLVSNLKKHYDSLPFSYSQADFDMKEVFLHVKLIEQAAGDDNPVFM 180

Query: 181 FQEVTNHDVQKPTMKLTFACNSSISWSTMSGALESADIRCEKIQIFEKKKFTLGVVLFVN 240
            QEV+  + +   ++LTFACNS +SWSTMSG L+SA I C+KIQIFEKK  TLGVVL ++
Sbjct: 181 IQEVSTEEPRGSVLRLTFACNSFLSWSTMSGVLDSASICCKKIQIFEKKGLTLGVVLLLD 240

Query: 241 LDAQEKVFKSKVENALKLAIKKPKTNTVKLPFGFCGCQEGNTRGKDLREIEEDAVEQNCR 300
              Q  +FK++VEN LK+A KKPK  +VKLPFG CGCQE N    +L  +EE++++ + R
Sbjct: 241 QSGQHSLFKTRVENTLKVATKKPKPTSVKLPFGLCGCQEQNGGVGELGGVEEESIQHSSR 300

Query: 301 SGFENSNLNENLQIEMPLSTSSFAVFVDEWQTVQSGGHELGKWLLSSENLELVDQIGPNS 360
            G E  NLN  +QI++PL +SSFAV VDEWQT+QSGG+E+GKWLL+S++ E  DQIGP S
Sbjct: 301 LGIE--NLNSTIQIQVPLPSSSFAVSVDEWQTIQSGGNEIGKWLLNSDSFEFGDQIGPTS 360

Query: 361 FKGVYKGKRVAIEKIKGCEKGVSYKFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVV 420
            KG+++GKRV IEK+KGC+KG SY+FELRKD LELM CGHK+IL FYGVCIDENHGLCVV
Sbjct: 361 LKGIFRGKRVGIEKLKGCDKGNSYEFELRKDYLELMACGHKSILQFYGVCIDENHGLCVV 420

Query: 421 TKLMEGGSVHELMLKNKRLQTKEITRIAIDVVEGIKFMNDHGVAYRDLNTQRILLDKNGN 480
           TKLMEGGS+HELMLKNK+LQTK+I RIAID+ EG+KF+NDHGVAYRDLNTQRILLDK+GN
Sbjct: 421 TKLMEGGSLHELMLKNKKLQTKQILRIAIDIAEGLKFVNDHGVAYRDLNTQRILLDKHGN 480

Query: 481 ACLGDMGILTACKNLGEAMEYETDGYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMV 540
           ACLG++GI+TACK+ GEA+EYETDGYRWLAPEIIAGDPE+  ETWMSN YSFGMV+WEMV
Sbjct: 481 ACLGNIGIVTACKSFGEAVEYETDGYRWLAPEIIAGDPENTTETWMSNAYSFGMVLWEMV 540

Query: 541 TGEAAYSAYSPVQAAVGIAACGLRPDVPKDCPSILKSLMIKCWNNCPNKRPQFSEILSVL 578
           TGEAAY++ SPVQAAVGIAACGLRP++PK+CP +L++LMI CWNN P+KRP FS I + L
Sbjct: 541 TGEAAYASCSPVQAAVGIAACGLRPEIPKECPQVLRTLMINCWNNSPSKRPNFSHIHNTL 599

BLAST of Spg001147 vs. TAIR 10
Match: AT5G07140.1 (Protein kinase superfamily protein )

HSP 1 Score: 766.5 bits (1978), Expect = 1.5e-221
Identity = 378/585 (64.62%), Postives = 465/585 (79.49%), Query Frame = 0

Query: 1   MATALECWSSR----ASTDEDLVEQVLMRTQDRSEG--SKPESSFAVGAKESSAMQKRLQ 60
           MA+ALECW++R       D+D V+QVLM ++DRSE   + P S      + SSAMQKR Q
Sbjct: 1   MASALECWTTRNAAGGCADDDFVDQVLMSSEDRSESLTAPPPSD-----QTSSAMQKRFQ 60

Query: 61  RLSRNVSEAVASIKNSLNLDSARDPSPTKTEGSKKAVWGSVVRNLTQLYPGSQLPEKLVS 120
           RL RNVS+A+AS+KNSLNLDS+RD     T G +K VW +VVRNL ++YPGSQLP+KLVS
Sbjct: 61  RLGRNVSDAIASLKNSLNLDSSRDNQNAATGGGRKLVWATVVRNLAKMYPGSQLPDKLVS 120

Query: 121 NIRKHYDSLPLSYAQAGFDMKDVFLHIKLIEQASVYDHPAILFQEVTNH--DVQKPTMKL 180
           N+RKHYDSLPLSY+Q GFDMKDVF+HIKLIEQA   D+P  + QEV +   D Q    KL
Sbjct: 121 NLRKHYDSLPLSYSQTGFDMKDVFVHIKLIEQAFGDDNPVFVIQEVCDEEADDQGSVFKL 180

Query: 181 TFACNSSISWSTMSGALESADIRCEKIQIFEKKKFTLGVVLFVNLDAQEKVFKSKVENAL 240
           TFAC  S+ WST+SG+L+ A I C+K+QIFEKK  TLGVVL +    QEK+FK KV NAL
Sbjct: 181 TFACTCSLPWSTISGSLDGALICCKKVQIFEKKGLTLGVVLLLVESGQEKMFKVKVVNAL 240

Query: 241 KLAIKKPKTNTVKLPFGFCGCQEGNTRGKDLREIEEDAVEQNCRSGFENSNLNENLQIEM 300
           + A++KPK+ +VKLPFG CGC+E N    +  +++ ++++Q  R   E  +LN  +Q++M
Sbjct: 241 RSAVRKPKSTSVKLPFGLCGCEEQNAGVGEFGDVDVESIDQCYR--HELDDLNTRIQLQM 300

Query: 301 PLSTSSFAVFVDEWQTVQSGGHELGKWLLSSENLELVDQIGPNSFKGVYKGKRVAIEKIK 360
           P  +SSF+V VDEWQT+QSGG ++ KWLL+S++LE   Q+GPNSFKGVY+G +VAIEK+K
Sbjct: 301 PPPSSSFSVSVDEWQTIQSGGDDIRKWLLNSDDLEFSGQLGPNSFKGVYRGTKVAIEKLK 360

Query: 361 GCEKGVSYKFELRKDLLELMTCGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKN 420
           GCEKG SY+F +RKD LELMTCGHK+IL FYGVCIDENHGLCVVTKLM+GGS+ EL+LK 
Sbjct: 361 GCEKGNSYEFAIRKDFLELMTCGHKSILQFYGVCIDENHGLCVVTKLMQGGSLRELVLKK 420

Query: 421 KRLQTKEITRIAIDVVEGIKFMNDHGVAYRDLNTQRILLDKNGNACLGDMGILTACKNLG 480
           K+LQTK I +IA+D+ EG+KF+NDHGVAYRDLNTQRILLDK  NACLGD+GI+TACK++ 
Sbjct: 421 KKLQTKLIFQIAVDIAEGMKFINDHGVAYRDLNTQRILLDKQCNACLGDLGIVTACKSVN 480

Query: 481 EAMEYETDGYRWLAPEIIAGDPESVNETWMSNVYSFGMVIWEMVTGEAAYSAYSPVQAAV 540
           EAMEYETDGYRWLAPEIIAGDPE   E+WMSN YSFGMV+WEMVTGE AY + SPVQAAV
Sbjct: 481 EAMEYETDGYRWLAPEIIAGDPEKTRESWMSNAYSFGMVLWEMVTGEEAYGSCSPVQAAV 540

Query: 541 GIAACGLRPDVPKDCPSILKSLMIKCWNNCPNKRPQFSEILSVLL 578
           GIAACGLRPD+PK+CP +LK LMIKCWN CP+ R  FS+I  +LL
Sbjct: 541 GIAACGLRPDIPKECPQVLKYLMIKCWNTCPSTRLNFSQIHCILL 578

BLAST of Spg001147 vs. TAIR 10
Match: AT4G35780.1 (ACT-like protein tyrosine kinase family protein )

HSP 1 Score: 162.9 bits (411), Expect = 7.6e-40
Identity = 84/264 (31.82%), Postives = 149/264 (56.44%), Query Frame = 0

Query: 318 KWLLSSENLELVDQIGPNS----FKGVYKGKRVAIEKIKGCEKGVSYKFELRKDLLELMT 377
           +W +  + L++  ++   S    F+G Y  + VAI+ +K          E  +++  +  
Sbjct: 284 EWEIDMKQLKIEKKVACGSYGELFRGTYCSQEVAIKILKPERVNAEMLREFSQEVYIMRK 343

Query: 378 CGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRL-QTKEITRIAIDVVEGIK 437
             HKN++ F G C   +  LC+VT+ M  GS+++ + K+K + + + + ++A+DV +G+ 
Sbjct: 344 VRHKNVVQFIGAC-TRSPNLCIVTEFMTRGSIYDFLHKHKGVFKIQSLLKVALDVSKGMN 403

Query: 438 FMNDHGVAYRDLNTQRILLDKNGNACLGDMGILTACKNLGEAMEYETDGYRWLAPEIIAG 497
           +++ + + +RDL T  +L+D++    + D G+       G  M  ET  YRW+APE+I  
Sbjct: 404 YLHQNNIIHRDLKTANLLMDEHEVVKVADFGVARVQTESG-VMTAETGTYRWMAPEVIEH 463

Query: 498 DPESVNETWMSNVYSFGMVIWEMVTGEAAYSAYSPVQAAVGIAACGLRPDVPKDCPSILK 557
            P        ++V+S+ +V+WE++TGE  YS  +P+QAAVG+   GLRP +PK+    L 
Sbjct: 464 KPYD----HRADVFSYAIVLWELLTGELPYSYLTPLQAAVGVVQKGLRPKIPKETHPKLT 523

Query: 558 SLMIKCWNNCPNKRPQFSEILSVL 577
            L+ KCW   P  RP F+EI+ +L
Sbjct: 524 ELLEKCWQQDPALRPNFAEIIEML 541

BLAST of Spg001147 vs. TAIR 10
Match: AT2G17700.1 (ACT-like protein tyrosine kinase family protein )

HSP 1 Score: 161.4 bits (407), Expect = 2.2e-39
Identity = 84/266 (31.58%), Postives = 151/266 (56.77%), Query Frame = 0

Query: 318 KWLLSSENLELVDQIGPNSF----KGVYKGKRVAIEKIKGCEKGVSYKFELRKDLLELMT 377
           +W +    L++  ++   S+    +G Y  + VAI+ +K          E  +++  +  
Sbjct: 278 EWEIDVTQLKIEKKVASGSYGDLHRGTYCSQEVAIKFLKPDRVNNEMLREFSQEVFIMRK 337

Query: 378 CGHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNK---RLQTKEITRIAIDVVEG 437
             HKN++ F G C   +  LC+VT+ M  GS+++ + K K   +LQT  + ++A+DV +G
Sbjct: 338 VRHKNVVQFLGAC-TRSPTLCIVTEFMARGSIYDFLHKQKCAFKLQT--LLKVALDVAKG 397

Query: 438 IKFMNDHGVAYRDLNTQRILLDKNGNACLGDMGILTACKNLGEAMEYETDGYRWLAPEII 497
           + +++ + + +RDL T  +L+D++G   + D G+       G  M  ET  YRW+APE+I
Sbjct: 398 MSYLHQNNIIHRDLKTANLLMDEHGLVKVADFGVARVQIESG-VMTAETGTYRWMAPEVI 457

Query: 498 AGDPESVNETWMSNVYSFGMVIWEMVTGEAAYSAYSPVQAAVGIAACGLRPDVPKDCPSI 557
              P +      ++V+S+ +V+WE++TG+  Y+  +P+QAAVG+   GLRP +PK     
Sbjct: 458 EHKPYN----HKADVFSYAIVLWELLTGDIPYAFLTPLQAAVGVVQKGLRPKIPKKTHPK 517

Query: 558 LKSLMIKCWNNCPNKRPQFSEILSVL 577
           +K L+ +CW+  P +RP F EI+ +L
Sbjct: 518 VKGLLERCWHQDPEQRPLFEEIIEML 535

BLAST of Spg001147 vs. TAIR 10
Match: AT4G38470.1 (ACT-like protein tyrosine kinase family protein )

HSP 1 Score: 161.0 bits (406), Expect = 2.9e-39
Identity = 85/263 (32.32%), Postives = 148/263 (56.27%), Query Frame = 0

Query: 319 WLLSSENLELVDQIGPNSFKGVYKG----KRVAIEKIKGCEKGVSYKFELRKDLLELMTC 378
           W ++ ++L+   +I   S+  +YKG    + VAI+ +K        + E  +++  +   
Sbjct: 283 WEINLKHLKFGHKIASGSYGDLYKGTYCSQEVAIKVLKPERLDSDLEKEFAQEVFIMRKV 342

Query: 379 GHKNILMFYGVCIDENHGLCVVTKLMEGGSVHELMLKNKRL-QTKEITRIAIDVVEGIKF 438
            HKN++ F G C    H LC+VT+ M GGSV++ + K K + +   + ++AID+ +G+ +
Sbjct: 343 RHKNVVQFIGACTKPPH-LCIVTEFMPGGSVYDYLHKQKGVFKLPTLFKVAIDICKGMSY 402

Query: 439 MNDHGVAYRDLNTQRILLDKNGNACLGDMGILTACKNLGEAMEYETDGYRWLAPEIIAGD 498
           ++ + + +RDL    +L+D+N    + D G+       G  M  ET  YRW+APE+I   
Sbjct: 403 LHQNNIIHRDLKAANLLMDENEVVKVADFGVARVKAQTG-VMTAETGTYRWMAPEVIEHK 462

Query: 499 PESVNETWMSNVYSFGMVIWEMVTGEAAYSAYSPVQAAVGIAACGLRPDVPKDCPSILKS 558
           P        ++V+S+G+V+WE++TG+  Y   +P+QAAVG+   GLRP +PK+    L  
Sbjct: 463 PYD----HKADVFSYGIVLWELLTGKLPYEYMTPLQAAVGVVQKGLRPTIPKNTHPKLAE 522

Query: 559 LMIKCWNNCPNKRPQFSEILSVL 577
           L+ + W +   +RP FSEI+  L
Sbjct: 523 LLERLWEHDSTQRPDFSEIIEQL 539

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7010490.10.0e+0094.36Serine/threonine-protein kinase STY46 [Cucurbita argyrosperma subsp. argyrosperm... [more]
XP_022148438.10.0e+0094.36dual specificity protein kinase shkB isoform X1 [Momordica charantia][more]
XP_022986818.10.0e+0094.02fibroblast growth factor receptor [Cucurbita maxima][more]
XP_023512978.10.0e+0093.85mitogen-activated protein kinase kinase kinase 13 [Cucurbita pepo subsp. pepo][more]
XP_038902013.10.0e+0094.19serine/threonine-protein kinase EDR1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q8RWL61.1e-3831.82Serine/threonine-protein kinase STY17 OS=Arabidopsis thaliana OX=3702 GN=STY17 P... [more]
O225583.1e-3831.58Serine/threonine-protein kinase STY8 OS=Arabidopsis thaliana OX=3702 GN=STY8 PE=... [more]
F4JTP54.1e-3832.32Serine/threonine-protein kinase STY46 OS=Arabidopsis thaliana OX=3702 GN=STY46 P... [more]
Q55GU02.0e-3231.30Probable serine/threonine-protein kinase DDB_G0267514 OS=Dictyostelium discoideu... [more]
Q7TQP62.6e-3232.71Serine/threonine-protein kinase TNNI3K OS=Rattus norvegicus OX=10116 GN=Tnni3k P... [more]
Match NameE-valueIdentityDescription
A0A6J1D4360.0e+0094.36dual specificity protein kinase shkB isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1JHM80.0e+0094.02fibroblast growth factor receptor OS=Cucurbita maxima OX=3661 GN=LOC111484449 PE... [more]
A0A6J1FY740.0e+0093.50mitogen-activated protein kinase kinase kinase 13 OS=Cucurbita moschata OX=3662 ... [more]
A0A0A0KC340.0e+0091.97Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G181... [more]
A0A6J1L4L50.0e+0092.31serine/threonine-protein kinase EDR1-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT5G58520.19.1e-23566.72Protein kinase superfamily protein [more]
AT5G07140.11.5e-22164.62Protein kinase superfamily protein [more]
AT4G35780.17.6e-4031.82ACT-like protein tyrosine kinase family protein [more]
AT2G17700.12.2e-3931.58ACT-like protein tyrosine kinase family protein [more]
AT4G38470.12.9e-3932.32ACT-like protein tyrosine kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPRINTSPR00109TYRKINASEcoord: 503..525
score: 32.42
coord: 397..410
score: 22.66
coord: 547..569
score: 43.06
coord: 433..451
score: 24.71
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 344..575
e-value: 7.3E-40
score: 136.9
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 319..582
e-value: 6.7E-52
score: 178.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 25..46
NoneNo IPR availablePANTHERPTHR23257:SF765PROTEIN KINASE SUPERFAMILY PROTEINcoord: 1..579
NoneNo IPR availablePANTHERPTHR23257SERINE-THREONINE PROTEIN KINASEcoord: 1..579
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 326..585
score: 26.887827
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 309..576

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg001147.1Spg001147.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004839 ubiquitin activating enzyme activity
molecular_function GO:0004672 protein kinase activity