Spg001034 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg001034 vs. NCBI nr
Match: XP_038902256.1 (chloride channel protein CLC-e [Benincasa hispida])

HSP 1 Score: 1128.2 bits (2917), Expect = 0.0e+00
Identity = 583/637 (91.52%), Postives = 610/637 (95.76%), Query Frame = 0

Query: 60  VHEIRDFSWDGIPNRGASWLREMPVEDIWKRVILVPACGGFLVSFLNLLRDATDVQVDRP 119
           VHEIRDFSWDGIPNRGASWLREMP++DIWKRVILVPACGGFLVSFLNLLRDATDV++++P
Sbjct: 159 VHEIRDFSWDGIPNRGASWLREMPIQDIWKRVILVPACGGFLVSFLNLLRDATDVKLEQP 218

Query: 120 QDDGSSIKFGVPVSISNKFKAALQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGIS 179
           Q DG S KFGVPVSISNKFKAALQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGIS
Sbjct: 219 QGDGPSTKFGVPVSISNKFKAALQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGIS 278

Query: 180 TVFEKNSRTKLSLIAAGSAAGISSGCDSSIFSGFNAAVAGCFFAVESVLWPSPADSTLSL 239
           TVF+KNSRTKLSLIAAGSAAGIS        SGFNAAVAGCFFA+ESVLWPSPADST+SL
Sbjct: 279 TVFDKNSRTKLSLIAAGSAAGIS--------SGFNAAVAGCFFAIESVLWPSPADSTISL 338

Query: 240 TNTTSMVILSAVIASVVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGVLCGLVSLGFSK 299
           TNTTSMVILSAVIASVVSQVGLGVEPAFKVP YDFRSPSELPLYLLLGVLCGLVSL FSK
Sbjct: 339 TNTTSMVILSAVIASVVSQVGLGVEPAFKVPVYDFRSPSELPLYLLLGVLCGLVSLSFSK 398

Query: 300 CTSYMLGTVDKFHKDFSVPRALFPILGGFTTGLIALAYPEILYWGFENVDLLLESRPFVK 359
           CTSYML TVDKFHKDF +P+ALFPILGGFTTGLIALAYPEILYWGFENVDLLLESRPFVK
Sbjct: 399 CTSYMLATVDKFHKDFGMPKALFPILGGFTTGLIALAYPEILYWGFENVDLLLESRPFVK 458

Query: 360 NLSAELLAQLVVIKVFATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNSVL 419
            LSAELLAQLVVIK+ ATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIG+ALSEPN+V+
Sbjct: 459 PLSAELLAQLVVIKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGLALSEPNTVI 518

Query: 420 DFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWIT 479
           DFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWIT
Sbjct: 519 DFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWIT 578

Query: 480 SGQKRKRSSQNTKKLPIGKSLSTQQSTTYDSNENDQSSNYADDGKATFPNDLCEIESSLC 539
           SGQKRKRSSQ TKKLP G+SL++QQST YDSN N+QSSNYADDG+  +PNDLCEIESSLC
Sbjct: 579 SGQKRKRSSQQTKKLPPGRSLTSQQSTAYDSNSNNQSSNYADDGQTNYPNDLCEIESSLC 638

Query: 540 AYDSDSDVVELERTISVSEAMTTRYVTVLMGTFLVEAVNLMLAEKQSCALIVDEENTLIG 599
           AYDSDS+ VELER I VSEAMTT+Y+TVLMGTFLVEAVNLMLAEKQSCALIVDEENTLIG
Sbjct: 639 AYDSDSETVELERKIFVSEAMTTKYITVLMGTFLVEAVNLMLAEKQSCALIVDEENTLIG 698

Query: 600 ILALEDIQKLSKNAKSRSEQLKELVVSEICSLDGKICRVPWTATPSMDILTAKMIMKNLG 659
           ILALEDIQKLSKNAKSRSE+LKELVVSEICSLDGKIC+VPWTATPSMDILTAKMIMKNLG
Sbjct: 699 ILALEDIQKLSKNAKSRSEELKELVVSEICSLDGKICQVPWTATPSMDILTAKMIMKNLG 758

Query: 660 VNQVPVVKDQMGYLVGVLDWECIDLTCRILATRESLG 697
           V QVPVV+DQMGYLVGVLDWECIDLTCRILATRESLG
Sbjct: 759 VTQVPVVRDQMGYLVGVLDWECIDLTCRILATRESLG 787

BLAST of Spg001034 vs. NCBI nr
Match: XP_022140763.1 (chloride channel protein CLC-e [Momordica charantia])

HSP 1 Score: 1109.7 bits (2869), Expect = 0.0e+00
Identity = 576/637 (90.42%), Postives = 601/637 (94.35%), Query Frame = 0

Query: 60  VHEIRDFSWDGIPNRGASWLREMPVEDIWKRVILVPACGGFLVSFLNLLRDATDVQVDRP 119
           VHEIR F WDGIPNRGASWLREMPVEDIWKRVILVPACGGFLVSFLNLLRDATDVQVDRP
Sbjct: 170 VHEIRGFFWDGIPNRGASWLREMPVEDIWKRVILVPACGGFLVSFLNLLRDATDVQVDRP 229

Query: 120 QDDGSSIKFGVPVSISNKFKAALQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGIS 179
           +  GSS KFGVP+S SNKFKAALQPFLKA+AAS+TLGTGNSLGPEGPSVDIGTSVGKGIS
Sbjct: 230 EKGGSSAKFGVPLSTSNKFKAALQPFLKAIAASITLGTGNSLGPEGPSVDIGTSVGKGIS 289

Query: 180 TVFEKNSRTKLSLIAAGSAAGISSGCDSSIFSGFNAAVAGCFFAVESVLWPSPADSTLSL 239
           +VF+KNS+TKLSLIAAGSAAGIS        SGFNAAVAGCFFAVESVLWPSPADSTLSL
Sbjct: 290 SVFDKNSQTKLSLIAAGSAAGIS--------SGFNAAVAGCFFAVESVLWPSPADSTLSL 349

Query: 240 TNTTSMVILSAVIASVVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGVLCGLVSLGFSK 299
           TNTTSMVILSAVIASVVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGVLCGLVSL FSK
Sbjct: 350 TNTTSMVILSAVIASVVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGVLCGLVSLSFSK 409

Query: 300 CTSYMLGTVDKFHKDFSVPRALFPILGGFTTGLIALAYPEILYWGFENVDLLLESRPFVK 359
           CTSYML T+DKFHKDF  PRALFP+LGGFTTGLIALAYPEILYWGFENVDLLLESRPFVK
Sbjct: 410 CTSYMLATIDKFHKDFGAPRALFPVLGGFTTGLIALAYPEILYWGFENVDLLLESRPFVK 469

Query: 360 NLSAELLAQLVVIKVFATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNSVL 419
            LSA+LLAQLVVIK+ ATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIA+S+ N ++
Sbjct: 470 GLSADLLAQLVVIKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIAVSDANPII 529

Query: 420 DFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWIT 479
           D SIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWIT
Sbjct: 530 DISIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWIT 589

Query: 480 SGQKRKRSSQNTKKLPIGKSLSTQQSTTYDSNENDQSSNYADDGKATFPNDLCEIESSLC 539
           SGQKRKR+SQ TKKLP+ +SL T+QST YDSN NDQSSNYADDGKAT  NDLCEIESSLC
Sbjct: 590 SGQKRKRTSQQTKKLPLERSLGTRQSTAYDSNANDQSSNYADDGKATNQNDLCEIESSLC 649

Query: 540 AYDSDSDVVELERTISVSEAMTTRYVTVLMGTFLVEAVNLMLAEKQSCALIVDEENTLIG 599
           AYDSDSD+VELER ISVSEAMTTRYVTVLMGTFLVEAVNLMLAEKQSCALIVDEENTLIG
Sbjct: 650 AYDSDSDIVELERKISVSEAMTTRYVTVLMGTFLVEAVNLMLAEKQSCALIVDEENTLIG 709

Query: 600 ILALEDIQKLSKNAKSRSEQLKELVVSEICSLDGKICRVPWTATPSMDILTAKMIMKNLG 659
           ILALEDIQKLSKNAKSRSE+LKE  VSEICSLDG+ICRVPWTATPSMD+LTA+MIMK LG
Sbjct: 710 ILALEDIQKLSKNAKSRSERLKEAAVSEICSLDGEICRVPWTATPSMDVLTARMIMKKLG 769

Query: 660 VNQVPVVKDQMGYLVGVLDWECIDLTCRILATRESLG 697
           V QVPVVKDQMGYLVGVL+WE IDLTCRILATRESLG
Sbjct: 770 VTQVPVVKDQMGYLVGVLEWEYIDLTCRILATRESLG 798

BLAST of Spg001034 vs. NCBI nr
Match: XP_031743789.1 (chloride channel protein CLC-e isoform X2 [Cucumis sativus])

HSP 1 Score: 1098.2 bits (2839), Expect = 0.0e+00
Identity = 572/637 (89.80%), Postives = 596/637 (93.56%), Query Frame = 0

Query: 60  VHEIRDFSWDGIPNRGASWLREMPVEDIWKRVILVPACGGFLVSFLNLLRDATDVQVDRP 119
           VHEIRDFSWDGIPNRGASWLREMP+EDIWKRVILVPA GGFLVSFLNLLRDATDV+VD+P
Sbjct: 20  VHEIRDFSWDGIPNRGASWLREMPIEDIWKRVILVPASGGFLVSFLNLLRDATDVKVDQP 79

Query: 120 QDDGSSIKFGVPVSISNKFKAALQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGIS 179
           Q D  S KFGVP+SISNK KAALQPFLKA+AASVTLGTGNSLGPEGPSVDIGTSVGKGIS
Sbjct: 80  QGDDPSTKFGVPISISNKLKAALQPFLKAIAASVTLGTGNSLGPEGPSVDIGTSVGKGIS 139

Query: 180 TVFEKNSRTKLSLIAAGSAAGISSGCDSSIFSGFNAAVAGCFFAVESVLWPSPADSTLSL 239
           TVFE NSRTKLSLIAAGSAAGIS        SGFNAAVAGCFFA+ESVLWPSPADST SL
Sbjct: 140 TVFEMNSRTKLSLIAAGSAAGIS--------SGFNAAVAGCFFAIESVLWPSPADSTFSL 199

Query: 240 TNTTSMVILSAVIASVVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGVLCGLVSLGFSK 299
           TNTTSMVILSAVIASVVSQVGLGVEPAFKVP YDFRSPSELPLYLLLGVLCGLVSL FSK
Sbjct: 200 TNTTSMVILSAVIASVVSQVGLGVEPAFKVPVYDFRSPSELPLYLLLGVLCGLVSLSFSK 259

Query: 300 CTSYMLGTVDKFHKDFSVPRALFPILGGFTTGLIALAYPEILYWGFENVDLLLESRPFVK 359
           CTSY+L TVDKFHK+F  PRA+FPILGGFT GLIALAYPEILYWGFENVDLLLESRPFVK
Sbjct: 260 CTSYLLATVDKFHKEFGAPRAMFPILGGFTIGLIALAYPEILYWGFENVDLLLESRPFVK 319

Query: 360 NLSAELLAQLVVIKVFATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNSVL 419
            LSAELLAQLVVIK+ ATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNSVL
Sbjct: 320 PLSAELLAQLVVIKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNSVL 379

Query: 420 DFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWIT 479
           DFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWIT
Sbjct: 380 DFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWIT 439

Query: 480 SGQKRKRSSQNTKKLPIGKSLSTQQSTTYDSNENDQSSNYADDGKATFPNDLCEIESSLC 539
           SGQKRKRSSQ TKKL  GKS STQQST YDSN N+QSSNYA+DG+  +PNDLCEIESSLC
Sbjct: 440 SGQKRKRSSQQTKKLSPGKSPSTQQSTAYDSNANNQSSNYAEDGQTNYPNDLCEIESSLC 499

Query: 540 AYDSDSDVVELERTISVSEAMTTRYVTVLMGTFLVEAVNLMLAEKQSCALIVDEENTLIG 599
           AY+SDS+ VELER ISVSEAMTT+Y+T+LMGT LVEAVNLMLAEKQSCALIVDE N LIG
Sbjct: 500 AYESDSETVELERKISVSEAMTTKYITILMGTCLVEAVNLMLAEKQSCALIVDEGNILIG 559

Query: 600 ILALEDIQKLSKNAKSRSEQLKELVVSEICSLDGKICRVPWTATPSMDILTAKMIMKNLG 659
           IL LEDIQKLSKNAKSR+EQLK+ VVSEICSLD K+CRVPWTATPSMDILTAKMIMKNLG
Sbjct: 560 ILELEDIQKLSKNAKSRNEQLKDFVVSEICSLDEKMCRVPWTATPSMDILTAKMIMKNLG 619

Query: 660 VNQVPVVKDQMGYLVGVLDWECIDLTCRILATRESLG 697
           V QVPVV+DQMGY+VGVLDWECIDLTCRILATRESLG
Sbjct: 620 VTQVPVVRDQMGYVVGVLDWECIDLTCRILATRESLG 648

BLAST of Spg001034 vs. NCBI nr
Match: XP_031743788.1 (chloride channel protein CLC-e isoform X1 [Cucumis sativus])

HSP 1 Score: 1098.2 bits (2839), Expect = 0.0e+00
Identity = 572/637 (89.80%), Postives = 596/637 (93.56%), Query Frame = 0

Query: 60  VHEIRDFSWDGIPNRGASWLREMPVEDIWKRVILVPACGGFLVSFLNLLRDATDVQVDRP 119
           VHEIRDFSWDGIPNRGASWLREMP+EDIWKRVILVPA GGFLVSFLNLLRDATDV+VD+P
Sbjct: 173 VHEIRDFSWDGIPNRGASWLREMPIEDIWKRVILVPASGGFLVSFLNLLRDATDVKVDQP 232

Query: 120 QDDGSSIKFGVPVSISNKFKAALQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGIS 179
           Q D  S KFGVP+SISNK KAALQPFLKA+AASVTLGTGNSLGPEGPSVDIGTSVGKGIS
Sbjct: 233 QGDDPSTKFGVPISISNKLKAALQPFLKAIAASVTLGTGNSLGPEGPSVDIGTSVGKGIS 292

Query: 180 TVFEKNSRTKLSLIAAGSAAGISSGCDSSIFSGFNAAVAGCFFAVESVLWPSPADSTLSL 239
           TVFE NSRTKLSLIAAGSAAGIS        SGFNAAVAGCFFA+ESVLWPSPADST SL
Sbjct: 293 TVFEMNSRTKLSLIAAGSAAGIS--------SGFNAAVAGCFFAIESVLWPSPADSTFSL 352

Query: 240 TNTTSMVILSAVIASVVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGVLCGLVSLGFSK 299
           TNTTSMVILSAVIASVVSQVGLGVEPAFKVP YDFRSPSELPLYLLLGVLCGLVSL FSK
Sbjct: 353 TNTTSMVILSAVIASVVSQVGLGVEPAFKVPVYDFRSPSELPLYLLLGVLCGLVSLSFSK 412

Query: 300 CTSYMLGTVDKFHKDFSVPRALFPILGGFTTGLIALAYPEILYWGFENVDLLLESRPFVK 359
           CTSY+L TVDKFHK+F  PRA+FPILGGFT GLIALAYPEILYWGFENVDLLLESRPFVK
Sbjct: 413 CTSYLLATVDKFHKEFGAPRAMFPILGGFTIGLIALAYPEILYWGFENVDLLLESRPFVK 472

Query: 360 NLSAELLAQLVVIKVFATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNSVL 419
            LSAELLAQLVVIK+ ATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNSVL
Sbjct: 473 PLSAELLAQLVVIKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNSVL 532

Query: 420 DFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWIT 479
           DFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWIT
Sbjct: 533 DFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWIT 592

Query: 480 SGQKRKRSSQNTKKLPIGKSLSTQQSTTYDSNENDQSSNYADDGKATFPNDLCEIESSLC 539
           SGQKRKRSSQ TKKL  GKS STQQST YDSN N+QSSNYA+DG+  +PNDLCEIESSLC
Sbjct: 593 SGQKRKRSSQQTKKLSPGKSPSTQQSTAYDSNANNQSSNYAEDGQTNYPNDLCEIESSLC 652

Query: 540 AYDSDSDVVELERTISVSEAMTTRYVTVLMGTFLVEAVNLMLAEKQSCALIVDEENTLIG 599
           AY+SDS+ VELER ISVSEAMTT+Y+T+LMGT LVEAVNLMLAEKQSCALIVDE N LIG
Sbjct: 653 AYESDSETVELERKISVSEAMTTKYITILMGTCLVEAVNLMLAEKQSCALIVDEGNILIG 712

Query: 600 ILALEDIQKLSKNAKSRSEQLKELVVSEICSLDGKICRVPWTATPSMDILTAKMIMKNLG 659
           IL LEDIQKLSKNAKSR+EQLK+ VVSEICSLD K+CRVPWTATPSMDILTAKMIMKNLG
Sbjct: 713 ILELEDIQKLSKNAKSRNEQLKDFVVSEICSLDEKMCRVPWTATPSMDILTAKMIMKNLG 772

Query: 660 VNQVPVVKDQMGYLVGVLDWECIDLTCRILATRESLG 697
           V QVPVV+DQMGY+VGVLDWECIDLTCRILATRESLG
Sbjct: 773 VTQVPVVRDQMGYVVGVLDWECIDLTCRILATRESLG 801

BLAST of Spg001034 vs. NCBI nr
Match: XP_008448151.1 (PREDICTED: chloride channel protein CLC-e [Cucumis melo])

HSP 1 Score: 1091.3 bits (2821), Expect = 0.0e+00
Identity = 569/638 (89.18%), Postives = 595/638 (93.26%), Query Frame = 0

Query: 60  VHEIRDFSWDGIPNRGASWLREMPVEDIWKRVILVPACGGFLVSFLNLLRDATDVQVDRP 119
           VHEIR+FSWDGIPNRGASWLREMP+EDIWKRVILVPA GG LVSFLNLLRDATDV+VD+P
Sbjct: 167 VHEIRNFSWDGIPNRGASWLREMPIEDIWKRVILVPASGGALVSFLNLLRDATDVKVDQP 226

Query: 120 QD-DGSSIKFGVPVSISNKFKAALQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGI 179
           Q+ D  S KFGVP+SISNK K ALQPFLKA+AASVTLGTGNSLGPEGPSVDIGTSVGKGI
Sbjct: 227 QEGDDPSTKFGVPISISNKLKVALQPFLKAIAASVTLGTGNSLGPEGPSVDIGTSVGKGI 286

Query: 180 STVFEKNSRTKLSLIAAGSAAGISSGCDSSIFSGFNAAVAGCFFAVESVLWPSPADSTLS 239
           STVFEKNSRTKLSLIAAGSAAGIS        SGFNAAVAGCFFA+ESVLWPSPADST S
Sbjct: 287 STVFEKNSRTKLSLIAAGSAAGIS--------SGFNAAVAGCFFAIESVLWPSPADSTFS 346

Query: 240 LTNTTSMVILSAVIASVVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGVLCGLVSLGFS 299
           LTNTTSMVILSAVIASVVSQVGLG EPAFKVP YDFRSPSELPLYLLLGVLCGLVSL FS
Sbjct: 347 LTNTTSMVILSAVIASVVSQVGLGAEPAFKVPVYDFRSPSELPLYLLLGVLCGLVSLSFS 406

Query: 300 KCTSYMLGTVDKFHKDFSVPRALFPILGGFTTGLIALAYPEILYWGFENVDLLLESRPFV 359
           KCTSY+L TVDKFHKDF  PRA+FPILGGFTTGLIALAYPEILYWGFENVDLLLESRPFV
Sbjct: 407 KCTSYLLATVDKFHKDFGAPRAMFPILGGFTTGLIALAYPEILYWGFENVDLLLESRPFV 466

Query: 360 KNLSAELLAQLVVIKVFATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNSV 419
           K LSAELLAQLVVIK+ ATSLCRA GLVGGYYAPSLFIGAATGMAYGKFIG+ALSEPNSV
Sbjct: 467 KPLSAELLAQLVVIKILATSLCRACGLVGGYYAPSLFIGAATGMAYGKFIGLALSEPNSV 526

Query: 420 LDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWI 479
           LDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWI
Sbjct: 527 LDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWI 586

Query: 480 TSGQKRKRSSQNTKKLPIGKSLSTQQSTTYDSNENDQSSNYADDGKATFPNDLCEIESSL 539
           TSGQKRKRSSQ TKKL  GKS STQQST YDSN N+QSSNYA+DG+  +PNDLCEIESSL
Sbjct: 587 TSGQKRKRSSQQTKKLSSGKSPSTQQSTAYDSNANNQSSNYAEDGQTNYPNDLCEIESSL 646

Query: 540 CAYDSDSDVVELERTISVSEAMTTRYVTVLMGTFLVEAVNLMLAEKQSCALIVDEENTLI 599
           CAY+SDS+ VELER ISVSEAMTT+Y+T+LMGT LVEAVNLMLAEKQSC LIVDE N LI
Sbjct: 647 CAYESDSETVELERKISVSEAMTTKYITILMGTCLVEAVNLMLAEKQSCVLIVDEGNILI 706

Query: 600 GILALEDIQKLSKNAKSRSEQLKELVVSEICSLDGKICRVPWTATPSMDILTAKMIMKNL 659
           GIL LE+IQKLSKNAKSR EQLK++VVSEICSLDGK+CRVPWTATPSMDILTAKMIMKNL
Sbjct: 707 GILELENIQKLSKNAKSRGEQLKDVVVSEICSLDGKMCRVPWTATPSMDILTAKMIMKNL 766

Query: 660 GVNQVPVVKDQMGYLVGVLDWECIDLTCRILATRESLG 697
           GV QVPVV+DQMGYLVGVLDWECIDLTCRILATRESLG
Sbjct: 767 GVTQVPVVRDQMGYLVGVLDWECIDLTCRILATRESLG 796

BLAST of Spg001034 vs. ExPASy Swiss-Prot
Match: Q8GX93 (Chloride channel protein CLC-e OS=Arabidopsis thaliana OX=3702 GN=CLC-E PE=2 SV=2)

HSP 1 Score: 709.1 bits (1829), Expect = 4.8e-203
Identity = 393/638 (61.60%), Postives = 471/638 (73.82%), Query Frame = 0

Query: 60  VHEIRDFSWDGIPNRGASWLREMPVEDIWKRVILVPACGGFLVSFLNLLRDATDVQVDRP 119
           VH +RDFSWDGIP+RGASWLRE P+   W RVILVP  GG +VS LN LR++        
Sbjct: 98  VHLLRDFSWDGIPDRGASWLREAPIGSNWLRVILVPTIGGLVVSILNQLRESAGKSTG-- 157

Query: 120 QDDGSSIKFGVPVSISNKFKAALQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGIS 179
            D  SS+         ++ KA L+PFLK VAA VTLGTGNSLGPEGPSV+IG S+ KG++
Sbjct: 158 -DSHSSL---------DRVKAVLRPFLKTVAACVTLGTGNSLGPEGPSVEIGASIAKGVN 217

Query: 180 TVFEKNSRTKLSLIAAGSAAGISSGCDSSIFSGFNAAVAGCFFAVESVLWPSPA-DSTLS 239
           ++F K+ +T  SL+AAGSAAGIS        SGFNAAVAGCFFAVESVLWPS + DS+ S
Sbjct: 218 SLFNKSPQTGFSLLAAGSAAGIS--------SGFNAAVAGCFFAVESVLWPSSSTDSSTS 277

Query: 240 LTNTTSMVILSAVIASVVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGVLCGLVSLGFS 299
           L NTTSMVILSAV ASVVS++GLG EPAFKVP+YDFRSP ELPLYLLLG LCGLVSL  S
Sbjct: 278 LPNTTSMVILSAVTASVVSEIGLGSEPAFKVPDYDFRSPGELPLYLLLGALCGLVSLALS 337

Query: 300 KCTSYMLGTVDKFHKDFSVPRALFPILGGFTTGLIALAYPEILYWGFENVDLLLESRPFV 359
           +CTS M   VD  +KD  +P+A+FP++GG + G+IAL YPE+LYWGF+NVD+LLE RPFV
Sbjct: 338 RCTSSMTSAVDSLNKDAGIPKAVFPVMGGLSVGIIALVYPEVLYWGFQNVDILLEKRPFV 397

Query: 360 KNLSAELLAQLVVIKVFATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNSV 419
           K LSA+LL QLV +K+ AT+ CRASGLVGGYYAPSLFIG A GMAYGKFIG+AL++ N  
Sbjct: 398 KGLSADLLLQLVAVKIAATAWCRASGLVGGYYAPSLFIGGAAGMAYGKFIGLALAQ-NPD 457

Query: 420 LDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWI 479
            + SI EVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVG+SSWI
Sbjct: 458 FNLSILEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGMSSWI 517

Query: 480 TSGQKRKRSSQNTKKLPIGKSLSTQQSTTYDSNENDQSSNYADDGKATFPNDLCEIESSL 539
           TSGQ +++ ++ TK+    KS    QS T   + +D+SS           N+LCE+ESSL
Sbjct: 518 TSGQSKRQETRETKETRKRKSQEAVQSLT---SSDDESST----------NNLCEVESSL 577

Query: 540 CAYDSDSDVVELERTISVSEAMTTRYVTVLMGTFLVEAVNLMLAEKQSCALIVDEENTLI 599
           C  DS +   EL ++I VSEAM TR+ TV+M T L EA+  ML EKQSCALIVD +N  +
Sbjct: 578 CLDDSLNQSEELPKSIFVSEAMRTRFATVMMSTSLEEALTRMLIEKQSCALIVDPDNIFL 637

Query: 600 GILALEDIQKLSKNAKSRSEQLKELVVSEICSLDGKICRVPWTATPSMDILTAKMIMKNL 659
           GIL L DIQ+ SK  K  + + K++ V++ICS  G  C+VPWT TP MD+L A+ IM   
Sbjct: 638 GILTLSDIQEFSKARKEGNNRPKDIFVNDICSRSGGKCKVPWTVTPDMDLLAAQTIMNKH 697

Query: 660 GVNQVPVVKDQMG----YLVGVLDWECIDLTCRILATR 693
            ++ V VV   +     + VGVLD ECI LT R LATR
Sbjct: 698 ELSHVAVVSGSIDAPRIHPVGVLDRECITLTRRALATR 701

BLAST of Spg001034 vs. ExPASy Swiss-Prot
Match: Q8RXR2 (Chloride channel protein CLC-f OS=Arabidopsis thaliana OX=3702 GN=CLC-F PE=2 SV=2)

HSP 1 Score: 401.0 bits (1029), Expect = 2.8e-110
Identity = 267/646 (41.33%), Postives = 375/646 (58.05%), Query Frame = 0

Query: 28  QFLPQMAPPTLSLMDSKIVKWVAAAMAV--YWVFVHEIRDFSWDGIPNRGASWLREMPVE 87
           + L   APP  +L+    +  VAA + V  +   VH I +++W G PN GA+WLR   + 
Sbjct: 119 EVLGDSAPPEWALLLIGCLIGVAAGICVAGFNKGVHVIHEWAWAGTPNEGAAWLRLQRLA 178

Query: 88  DIWKRVILVPACGGFLVSFLNLLRDATDVQVDRPQDDGSSIKFGVPVSISNKFKAALQPF 147
           D W R++L+P  GG +V  ++ L +     +D+ +   SS + G+       F A + P 
Sbjct: 179 DTWHRILLIPVTGGVIVGMMHGLLEI----LDQIRQSNSSQRQGL------DFLAGIYPV 238

Query: 148 LKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGISTVFEKNSRTKLSLIAAGSAAGISSGC 207
           +KA+ A+VTLGTG SLGPEGPSVDIG S   G + + E N   +++L AAG+A+GI+   
Sbjct: 239 IKAIQAAVTLGTGCSLGPEGPSVDIGKSCANGFALMMENNRERRIALTAAGAASGIA--- 298

Query: 208 DSSIFSGFNAAVAGCFFAVESVLWPSPADSTLSLTNTTSMVILSAVIASVVSQVGLGVEP 267
                SGFNAAVAGCFFA+E+VL P  A++  S   TT+M+IL++VI+S VS   LG + 
Sbjct: 299 -----SGFNAAVAGCFFAIETVLRPLRAEN--SPPFTTAMIILASVISSTVSNALLGTQS 358

Query: 268 AFKVPEYDFRSPSELPLYLLLGVLCGLVSLGFSKCTSYMLGTVDKFHKDFSVPRALFPIL 327
           AF VP YD +S +ELPLYL+LG+LCG VS+ FS+  ++   + D     F +P  + P L
Sbjct: 359 AFTVPSYDLKSAAELPLYLILGMLCGAVSVVFSRLVTWFTKSFDFIKDKFGLPAIVCPAL 418

Query: 328 GGFTTGLIALAYPEILYWGFENVDLLLESRPFVKNLSAELLAQLVVIKVFATSLCRASGL 387
           GG   G+IAL YP ILYWGF NV+ +L +          LLAQL   KV AT+LC+ SGL
Sbjct: 419 GGLGAGIIALKYPGILYWGFTNVEEILHTGKSASAPGIWLLAQLAAAKVVATALCKGSGL 478

Query: 388 VGGYYAPSLFIGAATGMAYGKFIGIALSEPNSVLDFSIFEVASPQAYGLVGMAATLAGVC 447
           VGG YAPSL IGAA G  +G   G A    N  +  +   VA PQAY LVGMAATLA +C
Sbjct: 479 VGGLYAPSLMIGAAVGAVFG---GSAAEIINRAIPGNA-AVAQPQAYALVGMAATLASMC 538

Query: 448 QVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWITSGQKRKRSSQNTKKLPIGKSLSTQQS 507
            VPLT+VLLLFELT+DYRI+LPL+GAVG++ W+ S   + + S +++    G+  S+   
Sbjct: 539 SVPLTSVLLLFELTKDYRILLPLMGAVGLAIWVPSVANQGKESDSSEGRSTGRGYSSLSP 598

Query: 508 TTYDSNENDQSSNYADDGKATFPNDLCEIESSLCAYDSDSDVVELERTISVSEAMTTRYV 567
           +   +    + ++ AD        +L  IE+    ++S  D   +   + V   M+  YV
Sbjct: 599 SERKTEGVWRHTDNADS------LELTVIENP--DHNSFLDEETILEDLKVMRVMSKNYV 658

Query: 568 TVLMGTFLVEAVNLMLAEKQSCALIVDEENTLIGILALEDIQKLSKNAKSRSEQLKELVV 627
            V  GT L EA N++    Q+C ++VD+++ L GIL   DI++   N  S         V
Sbjct: 659 KVSSGTTLREARNILKESHQNCIMVVDDDDFLAGILTHGDIRRYLSNNASTILDENTCPV 718

Query: 628 SEIC----SLDGKICRVPWTATPSMDILTAKMIMKNLGVNQVPVVK 668
           S +C    S  G+  R   T  P   +  AK +M+  GV Q+PVVK
Sbjct: 719 SSVCTKKISYRGQE-RGLLTCYPDATVGVAKELMEARGVKQLPVVK 731

BLAST of Spg001034 vs. ExPASy Swiss-Prot
Match: A5F0D5 (H(+)/Cl(-) exchange transporter ClcA OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395) OX=345073 GN=clcA PE=3 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 3.7e-30
Identity = 136/458 (29.69%), Postives = 208/458 (45.41%), Query Frame = 0

Query: 28  QFLPQMAPPTLSLMDSKIVKWVAAAMAVYW-VFVHEIRDFSWDGIPNRGASWLREMPVED 87
           QFL +   P   L  S +V  +A  +  Y+   VH + +   D + +   S+L       
Sbjct: 22  QFLSKDKTPFSVLFLSLLVGILAGLVGTYFEQAVHLVSETRTDWLKSEIGSFL------P 81

Query: 88  IWKRVILVPACGGFLVSFLNLLRDATDVQVDRPQDDGSSIKFGVPVSISNKFKAALQPF- 147
           +W    L+ A   F+  FL        V    P+  GS    G+P  I       ++P  
Sbjct: 82  LWLAAFLISAFLAFIGYFL--------VHRFAPEAAGS----GIP-EIEGAMD-GMRPVR 141

Query: 148 ------LKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGISTVFE-KNSRTKLSLIAAGSA 207
                 +K       LG+G  LG EGP+V +G +VG+ IS +F  KN  T+ SL+AAG+A
Sbjct: 142 WWRVLPVKFFGGMGALGSGMVLGREGPTVQMGGAVGRMISDIFRVKNEDTRHSLLAAGAA 201

Query: 208 AGISSGCDSSIFSGFNAAVAGCFFAVESVLWPSPADSTLSLTNTTSMVILSAVIASVVSQ 267
            G++        + FNA +AG  F +E  + P    + +S+      VI+SAV A++V +
Sbjct: 202 GGLA--------AAFNAPLAGIMFVIEE-MRPQFRYTLISV----RAVIISAVAANIVFR 261

Query: 268 VGLGVEPAFKVPEYDFRSPSELPLYLLLGVLCGLVSLGFSKCTSYMLGTVDKFHKDFSVP 327
           V  G +    +P+YD    S L L+LLLG L G+  + F+   +       KFH++    
Sbjct: 262 VINGQDAVITMPQYDAPELSTLGLFLLLGALFGVFGVLFNYLITLAQDLFVKFHRNDRKR 321

Query: 328 RALFPILGGFTTGLIALAYPEILYWGFENVDLLLESRPFVKNLSAELLAQLVVIKVFATS 387
             L   + G   GL+ L  PE+   G   +  +           A +L  L V ++F T 
Sbjct: 322 YLLTGSMIGGCFGLLLLYVPELTGGGISLIPTITNG-----GYGAGILLLLFVGRIFTTL 381

Query: 388 LCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNSVLDFSIFEVASPQAYGLVGMA 447
           LC  SG  GG +AP L +G   G A+G    +   E N            P  + + GM 
Sbjct: 382 LCFGSGAPGGIFAPMLALGTLFGYAFGLIAKVWFPELN----------IEPGMFAIAGMG 431

Query: 448 ATLAGVCQVPLTAVLLLFELTQDYRIVLPL----LGAV 473
           A  A   + P+T +LL+ E+T +Y ++LPL    LGAV
Sbjct: 442 ALFAATVRAPITGILLVIEMTNNYHLILPLIITSLGAV 431

BLAST of Spg001034 vs. ExPASy Swiss-Prot
Match: Q9KM62 (H(+)/Cl(-) exchange transporter ClcA OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) OX=243277 GN=clcA PE=3 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 6.4e-30
Identity = 136/458 (29.69%), Postives = 208/458 (45.41%), Query Frame = 0

Query: 28  QFLPQMAPPTLSLMDSKIVKWVAAAMAVYW-VFVHEIRDFSWDGIPNRGASWLREMPVED 87
           QFL +   P   L  S +V  +A  +  Y+   VH + +   D + +   S+L       
Sbjct: 22  QFLSKDKTPFSVLFLSLLVGILAGLVGTYFEQAVHLVSETRTDWLKSEIGSFL------P 81

Query: 88  IWKRVILVPACGGFLVSFLNLLRDATDVQVDRPQDDGSSIKFGVPVSISNKFKAALQPF- 147
           +W    L+ A   F+  FL        V    P+  GS    G+P  I       ++P  
Sbjct: 82  LWLAAFLISAFLAFIGYFL--------VHRFAPEAAGS----GIP-EIEGAMD-GMRPVR 141

Query: 148 ------LKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGISTVFE-KNSRTKLSLIAAGSA 207
                 +K       LG+G  LG EGP+V +G +VG+ IS +F  KN  T+ SL+AAG+A
Sbjct: 142 WWRVLPVKFFGGMGALGSGMVLGREGPTVQMGGAVGRMISDIFRVKNEDTRHSLLAAGAA 201

Query: 208 AGISSGCDSSIFSGFNAAVAGCFFAVESVLWPSPADSTLSLTNTTSMVILSAVIASVVSQ 267
            G++        + FNA +AG  F +E  + P    + +S+      VI+SAV A++V +
Sbjct: 202 GGLA--------AAFNAPLAGIMFVIEE-MRPQFRYTLISV----RAVIISAVAANIVFR 261

Query: 268 VGLGVEPAFKVPEYDFRSPSELPLYLLLGVLCGLVSLGFSKCTSYMLGTVDKFHKDFSVP 327
           V  G +    +P+YD    S L L+LLLG L G+  + F+   +       KFH++    
Sbjct: 262 VINGQDAVITMPQYDAPELSTLGLFLLLGALFGVFGVLFNYLITLAQDLFVKFHRNDRKR 321

Query: 328 RALFPILGGFTTGLIALAYPEILYWGFENVDLLLESRPFVKNLSAELLAQLVVIKVFATS 387
             L   + G   GL+ L  PE+   G   +  +           A +L  L V ++F T 
Sbjct: 322 YLLTGSMIGGCFGLLLLYVPELTGGGISLIPTITNG-----GYGAGILLLLFVGRIFTTL 381

Query: 388 LCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNSVLDFSIFEVASPQAYGLVGMA 447
           LC  SG  GG +AP L +G   G A+G    +   E N            P  + + GM 
Sbjct: 382 LCFGSGAPGGIFAPMLALGTLFGYAFGLIAKMWFPELN----------IEPGMFAIAGMG 431

Query: 448 ATLAGVCQVPLTAVLLLFELTQDYRIVLPL----LGAV 473
           A  A   + P+T +LL+ E+T +Y ++LPL    LGAV
Sbjct: 442 ALFAATVRAPITGILLVIEMTNNYHLILPLIITSLGAV 431

BLAST of Spg001034 vs. ExPASy Swiss-Prot
Match: C3LVE3 (H(+)/Cl(-) exchange transporter ClcA OS=Vibrio cholerae serotype O1 (strain M66-2) OX=579112 GN=clcA PE=3 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 6.4e-30
Identity = 136/458 (29.69%), Postives = 208/458 (45.41%), Query Frame = 0

Query: 28  QFLPQMAPPTLSLMDSKIVKWVAAAMAVYW-VFVHEIRDFSWDGIPNRGASWLREMPVED 87
           QFL +   P   L  S +V  +A  +  Y+   VH + +   D + +   S+L       
Sbjct: 22  QFLSKDKTPFSVLFLSLLVGILAGLVGTYFEQAVHLVSETRTDWLKSEIGSFL------P 81

Query: 88  IWKRVILVPACGGFLVSFLNLLRDATDVQVDRPQDDGSSIKFGVPVSISNKFKAALQPF- 147
           +W    L+ A   F+  FL        V    P+  GS    G+P  I       ++P  
Sbjct: 82  LWLAAFLISAFLAFIGYFL--------VHRFAPEAAGS----GIP-EIEGAMD-GMRPVR 141

Query: 148 ------LKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGISTVFE-KNSRTKLSLIAAGSA 207
                 +K       LG+G  LG EGP+V +G +VG+ IS +F  KN  T+ SL+AAG+A
Sbjct: 142 WWRVLPVKFFGGMGALGSGMVLGREGPTVQMGGAVGRMISDIFRVKNEDTRHSLLAAGAA 201

Query: 208 AGISSGCDSSIFSGFNAAVAGCFFAVESVLWPSPADSTLSLTNTTSMVILSAVIASVVSQ 267
            G++        + FNA +AG  F +E  + P    + +S+      VI+SAV A++V +
Sbjct: 202 GGLA--------AAFNAPLAGIMFVIEE-MRPQFRYTLISV----RAVIISAVAANIVFR 261

Query: 268 VGLGVEPAFKVPEYDFRSPSELPLYLLLGVLCGLVSLGFSKCTSYMLGTVDKFHKDFSVP 327
           V  G +    +P+YD    S L L+LLLG L G+  + F+   +       KFH++    
Sbjct: 262 VINGQDAVITMPQYDAPELSTLGLFLLLGALFGVFGVLFNYLITLAQDLFVKFHRNDRKR 321

Query: 328 RALFPILGGFTTGLIALAYPEILYWGFENVDLLLESRPFVKNLSAELLAQLVVIKVFATS 387
             L   + G   GL+ L  PE+   G   +  +           A +L  L V ++F T 
Sbjct: 322 YLLTGSMIGGCFGLLLLYVPELTGGGISLIPTITNG-----GYGAGILLLLFVGRIFTTL 381

Query: 388 LCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNSVLDFSIFEVASPQAYGLVGMA 447
           LC  SG  GG +AP L +G   G A+G    +   E N            P  + + GM 
Sbjct: 382 LCFGSGAPGGIFAPMLALGTLFGYAFGLIAKMWFPELN----------IEPGMFAIAGMG 431

Query: 448 ATLAGVCQVPLTAVLLLFELTQDYRIVLPL----LGAV 473
           A  A   + P+T +LL+ E+T +Y ++LPL    LGAV
Sbjct: 442 ALFAATVRAPITGILLVIEMTNNYHLILPLIITSLGAV 431

BLAST of Spg001034 vs. ExPASy TrEMBL
Match: A0A6J1CIR4 (chloride channel protein CLC-e OS=Momordica charantia OX=3673 GN=LOC111011346 PE=3 SV=1)

HSP 1 Score: 1109.7 bits (2869), Expect = 0.0e+00
Identity = 576/637 (90.42%), Postives = 601/637 (94.35%), Query Frame = 0

Query: 60  VHEIRDFSWDGIPNRGASWLREMPVEDIWKRVILVPACGGFLVSFLNLLRDATDVQVDRP 119
           VHEIR F WDGIPNRGASWLREMPVEDIWKRVILVPACGGFLVSFLNLLRDATDVQVDRP
Sbjct: 170 VHEIRGFFWDGIPNRGASWLREMPVEDIWKRVILVPACGGFLVSFLNLLRDATDVQVDRP 229

Query: 120 QDDGSSIKFGVPVSISNKFKAALQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGIS 179
           +  GSS KFGVP+S SNKFKAALQPFLKA+AAS+TLGTGNSLGPEGPSVDIGTSVGKGIS
Sbjct: 230 EKGGSSAKFGVPLSTSNKFKAALQPFLKAIAASITLGTGNSLGPEGPSVDIGTSVGKGIS 289

Query: 180 TVFEKNSRTKLSLIAAGSAAGISSGCDSSIFSGFNAAVAGCFFAVESVLWPSPADSTLSL 239
           +VF+KNS+TKLSLIAAGSAAGIS        SGFNAAVAGCFFAVESVLWPSPADSTLSL
Sbjct: 290 SVFDKNSQTKLSLIAAGSAAGIS--------SGFNAAVAGCFFAVESVLWPSPADSTLSL 349

Query: 240 TNTTSMVILSAVIASVVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGVLCGLVSLGFSK 299
           TNTTSMVILSAVIASVVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGVLCGLVSL FSK
Sbjct: 350 TNTTSMVILSAVIASVVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGVLCGLVSLSFSK 409

Query: 300 CTSYMLGTVDKFHKDFSVPRALFPILGGFTTGLIALAYPEILYWGFENVDLLLESRPFVK 359
           CTSYML T+DKFHKDF  PRALFP+LGGFTTGLIALAYPEILYWGFENVDLLLESRPFVK
Sbjct: 410 CTSYMLATIDKFHKDFGAPRALFPVLGGFTTGLIALAYPEILYWGFENVDLLLESRPFVK 469

Query: 360 NLSAELLAQLVVIKVFATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNSVL 419
            LSA+LLAQLVVIK+ ATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIA+S+ N ++
Sbjct: 470 GLSADLLAQLVVIKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIAVSDANPII 529

Query: 420 DFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWIT 479
           D SIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWIT
Sbjct: 530 DISIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWIT 589

Query: 480 SGQKRKRSSQNTKKLPIGKSLSTQQSTTYDSNENDQSSNYADDGKATFPNDLCEIESSLC 539
           SGQKRKR+SQ TKKLP+ +SL T+QST YDSN NDQSSNYADDGKAT  NDLCEIESSLC
Sbjct: 590 SGQKRKRTSQQTKKLPLERSLGTRQSTAYDSNANDQSSNYADDGKATNQNDLCEIESSLC 649

Query: 540 AYDSDSDVVELERTISVSEAMTTRYVTVLMGTFLVEAVNLMLAEKQSCALIVDEENTLIG 599
           AYDSDSD+VELER ISVSEAMTTRYVTVLMGTFLVEAVNLMLAEKQSCALIVDEENTLIG
Sbjct: 650 AYDSDSDIVELERKISVSEAMTTRYVTVLMGTFLVEAVNLMLAEKQSCALIVDEENTLIG 709

Query: 600 ILALEDIQKLSKNAKSRSEQLKELVVSEICSLDGKICRVPWTATPSMDILTAKMIMKNLG 659
           ILALEDIQKLSKNAKSRSE+LKE  VSEICSLDG+ICRVPWTATPSMD+LTA+MIMK LG
Sbjct: 710 ILALEDIQKLSKNAKSRSERLKEAAVSEICSLDGEICRVPWTATPSMDVLTARMIMKKLG 769

Query: 660 VNQVPVVKDQMGYLVGVLDWECIDLTCRILATRESLG 697
           V QVPVVKDQMGYLVGVL+WE IDLTCRILATRESLG
Sbjct: 770 VTQVPVVKDQMGYLVGVLEWEYIDLTCRILATRESLG 798

BLAST of Spg001034 vs. ExPASy TrEMBL
Match: A0A0A0KAV3 (Chloride channel protein OS=Cucumis sativus OX=3659 GN=Csa_6G116660 PE=3 SV=1)

HSP 1 Score: 1098.2 bits (2839), Expect = 0.0e+00
Identity = 572/637 (89.80%), Postives = 596/637 (93.56%), Query Frame = 0

Query: 60  VHEIRDFSWDGIPNRGASWLREMPVEDIWKRVILVPACGGFLVSFLNLLRDATDVQVDRP 119
           VHEIRDFSWDGIPNRGASWLREMP+EDIWKRVILVPA GGFLVSFLNLLRDATDV+VD+P
Sbjct: 324 VHEIRDFSWDGIPNRGASWLREMPIEDIWKRVILVPASGGFLVSFLNLLRDATDVKVDQP 383

Query: 120 QDDGSSIKFGVPVSISNKFKAALQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGIS 179
           Q D  S KFGVP+SISNK KAALQPFLKA+AASVTLGTGNSLGPEGPSVDIGTSVGKGIS
Sbjct: 384 QGDDPSTKFGVPISISNKLKAALQPFLKAIAASVTLGTGNSLGPEGPSVDIGTSVGKGIS 443

Query: 180 TVFEKNSRTKLSLIAAGSAAGISSGCDSSIFSGFNAAVAGCFFAVESVLWPSPADSTLSL 239
           TVFE NSRTKLSLIAAGSAAGIS        SGFNAAVAGCFFA+ESVLWPSPADST SL
Sbjct: 444 TVFEMNSRTKLSLIAAGSAAGIS--------SGFNAAVAGCFFAIESVLWPSPADSTFSL 503

Query: 240 TNTTSMVILSAVIASVVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGVLCGLVSLGFSK 299
           TNTTSMVILSAVIASVVSQVGLGVEPAFKVP YDFRSPSELPLYLLLGVLCGLVSL FSK
Sbjct: 504 TNTTSMVILSAVIASVVSQVGLGVEPAFKVPVYDFRSPSELPLYLLLGVLCGLVSLSFSK 563

Query: 300 CTSYMLGTVDKFHKDFSVPRALFPILGGFTTGLIALAYPEILYWGFENVDLLLESRPFVK 359
           CTSY+L TVDKFHK+F  PRA+FPILGGFT GLIALAYPEILYWGFENVDLLLESRPFVK
Sbjct: 564 CTSYLLATVDKFHKEFGAPRAMFPILGGFTIGLIALAYPEILYWGFENVDLLLESRPFVK 623

Query: 360 NLSAELLAQLVVIKVFATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNSVL 419
            LSAELLAQLVVIK+ ATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNSVL
Sbjct: 624 PLSAELLAQLVVIKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNSVL 683

Query: 420 DFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWIT 479
           DFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWIT
Sbjct: 684 DFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWIT 743

Query: 480 SGQKRKRSSQNTKKLPIGKSLSTQQSTTYDSNENDQSSNYADDGKATFPNDLCEIESSLC 539
           SGQKRKRSSQ TKKL  GKS STQQST YDSN N+QSSNYA+DG+  +PNDLCEIESSLC
Sbjct: 744 SGQKRKRSSQQTKKLSPGKSPSTQQSTAYDSNANNQSSNYAEDGQTNYPNDLCEIESSLC 803

Query: 540 AYDSDSDVVELERTISVSEAMTTRYVTVLMGTFLVEAVNLMLAEKQSCALIVDEENTLIG 599
           AY+SDS+ VELER ISVSEAMTT+Y+T+LMGT LVEAVNLMLAEKQSCALIVDE N LIG
Sbjct: 804 AYESDSETVELERKISVSEAMTTKYITILMGTCLVEAVNLMLAEKQSCALIVDEGNILIG 863

Query: 600 ILALEDIQKLSKNAKSRSEQLKELVVSEICSLDGKICRVPWTATPSMDILTAKMIMKNLG 659
           IL LEDIQKLSKNAKSR+EQLK+ VVSEICSLD K+CRVPWTATPSMDILTAKMIMKNLG
Sbjct: 864 ILELEDIQKLSKNAKSRNEQLKDFVVSEICSLDEKMCRVPWTATPSMDILTAKMIMKNLG 923

Query: 660 VNQVPVVKDQMGYLVGVLDWECIDLTCRILATRESLG 697
           V QVPVV+DQMGY+VGVLDWECIDLTCRILATRESLG
Sbjct: 924 VTQVPVVRDQMGYVVGVLDWECIDLTCRILATRESLG 952

BLAST of Spg001034 vs. ExPASy TrEMBL
Match: A0A1S3BJ09 (Chloride channel protein OS=Cucumis melo OX=3656 GN=LOC103490437 PE=3 SV=1)

HSP 1 Score: 1091.3 bits (2821), Expect = 0.0e+00
Identity = 569/638 (89.18%), Postives = 595/638 (93.26%), Query Frame = 0

Query: 60  VHEIRDFSWDGIPNRGASWLREMPVEDIWKRVILVPACGGFLVSFLNLLRDATDVQVDRP 119
           VHEIR+FSWDGIPNRGASWLREMP+EDIWKRVILVPA GG LVSFLNLLRDATDV+VD+P
Sbjct: 167 VHEIRNFSWDGIPNRGASWLREMPIEDIWKRVILVPASGGALVSFLNLLRDATDVKVDQP 226

Query: 120 QD-DGSSIKFGVPVSISNKFKAALQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGI 179
           Q+ D  S KFGVP+SISNK K ALQPFLKA+AASVTLGTGNSLGPEGPSVDIGTSVGKGI
Sbjct: 227 QEGDDPSTKFGVPISISNKLKVALQPFLKAIAASVTLGTGNSLGPEGPSVDIGTSVGKGI 286

Query: 180 STVFEKNSRTKLSLIAAGSAAGISSGCDSSIFSGFNAAVAGCFFAVESVLWPSPADSTLS 239
           STVFEKNSRTKLSLIAAGSAAGIS        SGFNAAVAGCFFA+ESVLWPSPADST S
Sbjct: 287 STVFEKNSRTKLSLIAAGSAAGIS--------SGFNAAVAGCFFAIESVLWPSPADSTFS 346

Query: 240 LTNTTSMVILSAVIASVVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGVLCGLVSLGFS 299
           LTNTTSMVILSAVIASVVSQVGLG EPAFKVP YDFRSPSELPLYLLLGVLCGLVSL FS
Sbjct: 347 LTNTTSMVILSAVIASVVSQVGLGAEPAFKVPVYDFRSPSELPLYLLLGVLCGLVSLSFS 406

Query: 300 KCTSYMLGTVDKFHKDFSVPRALFPILGGFTTGLIALAYPEILYWGFENVDLLLESRPFV 359
           KCTSY+L TVDKFHKDF  PRA+FPILGGFTTGLIALAYPEILYWGFENVDLLLESRPFV
Sbjct: 407 KCTSYLLATVDKFHKDFGAPRAMFPILGGFTTGLIALAYPEILYWGFENVDLLLESRPFV 466

Query: 360 KNLSAELLAQLVVIKVFATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNSV 419
           K LSAELLAQLVVIK+ ATSLCRA GLVGGYYAPSLFIGAATGMAYGKFIG+ALSEPNSV
Sbjct: 467 KPLSAELLAQLVVIKILATSLCRACGLVGGYYAPSLFIGAATGMAYGKFIGLALSEPNSV 526

Query: 420 LDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWI 479
           LDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWI
Sbjct: 527 LDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWI 586

Query: 480 TSGQKRKRSSQNTKKLPIGKSLSTQQSTTYDSNENDQSSNYADDGKATFPNDLCEIESSL 539
           TSGQKRKRSSQ TKKL  GKS STQQST YDSN N+QSSNYA+DG+  +PNDLCEIESSL
Sbjct: 587 TSGQKRKRSSQQTKKLSSGKSPSTQQSTAYDSNANNQSSNYAEDGQTNYPNDLCEIESSL 646

Query: 540 CAYDSDSDVVELERTISVSEAMTTRYVTVLMGTFLVEAVNLMLAEKQSCALIVDEENTLI 599
           CAY+SDS+ VELER ISVSEAMTT+Y+T+LMGT LVEAVNLMLAEKQSC LIVDE N LI
Sbjct: 647 CAYESDSETVELERKISVSEAMTTKYITILMGTCLVEAVNLMLAEKQSCVLIVDEGNILI 706

Query: 600 GILALEDIQKLSKNAKSRSEQLKELVVSEICSLDGKICRVPWTATPSMDILTAKMIMKNL 659
           GIL LE+IQKLSKNAKSR EQLK++VVSEICSLDGK+CRVPWTATPSMDILTAKMIMKNL
Sbjct: 707 GILELENIQKLSKNAKSRGEQLKDVVVSEICSLDGKMCRVPWTATPSMDILTAKMIMKNL 766

Query: 660 GVNQVPVVKDQMGYLVGVLDWECIDLTCRILATRESLG 697
           GV QVPVV+DQMGYLVGVLDWECIDLTCRILATRESLG
Sbjct: 767 GVTQVPVVRDQMGYLVGVLDWECIDLTCRILATRESLG 796

BLAST of Spg001034 vs. ExPASy TrEMBL
Match: A0A5D3BPS9 (Chloride channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold264G00820 PE=3 SV=1)

HSP 1 Score: 1072.4 bits (2772), Expect = 7.8e-310
Identity = 559/630 (88.73%), Postives = 586/630 (93.02%), Query Frame = 0

Query: 58  VFVHEIRDFSWDGIPNRGASWLREMPVEDIWKRVILVPACGGFLVSFLNLLRDATDVQVD 117
           + VHEIR+FSWDGIPNRGASWLREMP+EDIWKRVILVPA GG LVSFLNLLRDATDV+VD
Sbjct: 105 ISVHEIRNFSWDGIPNRGASWLREMPIEDIWKRVILVPASGGALVSFLNLLRDATDVKVD 164

Query: 118 RPQD-DGSSIKFGVPVSISNKFKAALQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGK 177
           +PQ+ D  S KFGVP+SISNK K ALQPFLKA+AASVTLGTGNSLGPEGPSVDIGTSVGK
Sbjct: 165 QPQEGDDPSTKFGVPISISNKLKVALQPFLKAIAASVTLGTGNSLGPEGPSVDIGTSVGK 224

Query: 178 GISTVFEKNSRTKLSLIAAGSAAGISSGCDSSIFSGFNAAVAGCFFAVESVLWPSPADST 237
           GISTVFEKNSRTKLSLIAAGSAAGIS        SGFNAAVAGCFFA+ESVLWPSPADST
Sbjct: 225 GISTVFEKNSRTKLSLIAAGSAAGIS--------SGFNAAVAGCFFAIESVLWPSPADST 284

Query: 238 LSLTNTTSMVILSAVIASVVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGVLCGLVSLG 297
            SLTNTTSMVILSAVIASVVSQVGLG EPAFKVP YDFRSPSELPLYLLLGVLCGLVSL 
Sbjct: 285 FSLTNTTSMVILSAVIASVVSQVGLGAEPAFKVPVYDFRSPSELPLYLLLGVLCGLVSLS 344

Query: 298 FSKCTSYMLGTVDKFHKDFSVPRALFPILGGFTTGLIALAYPEILYWGFENVDLLLESRP 357
           FSKCTSY+L TVDKFHKDF  PRA+FPILGGFTTGLIALAYPEILYWGFENVDLLLESRP
Sbjct: 345 FSKCTSYLLATVDKFHKDFGAPRAMFPILGGFTTGLIALAYPEILYWGFENVDLLLESRP 404

Query: 358 FVKNLSAELLAQLVVIKVFATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPN 417
           FVK LSAELLAQLVVIK+ ATSLCRA GLVGGYYAPSLFIGAATGMAYGKFIG+ALSEPN
Sbjct: 405 FVKPLSAELLAQLVVIKILATSLCRACGLVGGYYAPSLFIGAATGMAYGKFIGLALSEPN 464

Query: 418 SVLDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSS 477
           SVLDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSS
Sbjct: 465 SVLDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSS 524

Query: 478 WITSGQKRKRSSQNTKKLPIGKSLSTQQSTTYDSNENDQSSNYADDGKATFPNDLCEIES 537
           WITSGQKRKRSSQ TKKL  GKS STQQST YDSN N+QSSNYA+DG+  +PNDLCEIES
Sbjct: 525 WITSGQKRKRSSQQTKKLSSGKSPSTQQSTAYDSNANNQSSNYAEDGQTNYPNDLCEIES 584

Query: 538 SLCAYDSDSDVVELERTISVSEAMTTRYVTVLMGTFLVEAVNLMLAEKQSCALIVDEENT 597
           SLCAY+SDS+ VELER ISVSEAMTT+Y+T+LMGT LVEAVNLMLAEKQSC LIVDE N 
Sbjct: 585 SLCAYESDSETVELERKISVSEAMTTKYITILMGTCLVEAVNLMLAEKQSCVLIVDEGNI 644

Query: 598 LIGILALEDIQKLSKNAKSRSEQLKELVVSEICSLDGKICRVPWTATPSMDILTAKMIMK 657
           LIGIL LE+IQKLSKNAKSR EQLK++VVSEICSLDGK+CRVPWTATPSMDILTAKMIMK
Sbjct: 645 LIGILELENIQKLSKNAKSRGEQLKDVVVSEICSLDGKMCRVPWTATPSMDILTAKMIMK 704

Query: 658 NLGVNQVPVVKDQMGYLVGVLDWECIDLTC 687
           NLGV QVPVV+DQMGYLVGVLDWECIDLTC
Sbjct: 705 NLGVTQVPVVRDQMGYLVGVLDWECIDLTC 726

BLAST of Spg001034 vs. ExPASy TrEMBL
Match: A0A6J1I747 (Chloride channel protein OS=Cucurbita maxima OX=3661 GN=LOC111469757 PE=3 SV=1)

HSP 1 Score: 1071.6 bits (2770), Expect = 1.3e-309
Identity = 562/637 (88.23%), Postives = 590/637 (92.62%), Query Frame = 0

Query: 60  VHEIRDFSWDGIPNRGASWLREMPVEDIWKRVILVPACGGFLVSFLNLLRDATDVQVDRP 119
           VHE+RDF WDGIPNRGASWLREMPVE+ WKRVILVPACGGFLVSFLNLLR+AT       
Sbjct: 150 VHELRDFFWDGIPNRGASWLREMPVEETWKRVILVPACGGFLVSFLNLLREAT------- 209

Query: 120 QDDGSSIKFGVPVSISNKFKAALQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGIS 179
            +   S K GVPVSISN+F+AALQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKG+S
Sbjct: 210 -NSDPSTKLGVPVSISNQFRAALQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGVS 269

Query: 180 TVFEKNSRTKLSLIAAGSAAGISSGCDSSIFSGFNAAVAGCFFAVESVLWPSPADSTLSL 239
           TVF++NSRTKLSLIAAGSAAGIS        SGFNAAVAGCFFAVESVLWPSPADST SL
Sbjct: 270 TVFDRNSRTKLSLIAAGSAAGIS--------SGFNAAVAGCFFAVESVLWPSPADSTFSL 329

Query: 240 TNTTSMVILSAVIASVVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGVLCGLVSLGFSK 299
           TNTTSMVILSAVIAS VSQVGLGVEPAFKVPEYDFRSPSELPLYLLLG LCGLVSL FSK
Sbjct: 330 TNTTSMVILSAVIASAVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGALCGLVSLSFSK 389

Query: 300 CTSYMLGTVDKFHKDFSVPRALFPILGGFTTGLIALAYPEILYWGFENVDLLLESRPFVK 359
           CTSYML TVDK HKDF V RALFPILGGF+TGLIA+AYPEILYWGFENVDLLLESRPFVK
Sbjct: 390 CTSYMLATVDKVHKDFGVSRALFPILGGFSTGLIAMAYPEILYWGFENVDLLLESRPFVK 449

Query: 360 NLSAELLAQLVVIKVFATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNSVL 419
            LSAELLAQLVV+K+ ATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPN+V+
Sbjct: 450 PLSAELLAQLVVVKILATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNTVI 509

Query: 420 DFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWIT 479
           DFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSW+T
Sbjct: 510 DFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWLT 569

Query: 480 SGQKRKRSSQNTKKLPIGKSLSTQQSTTYDSNENDQSSNYADDGKATFPNDLCEIESSLC 539
           SGQKRKRSSQ TKKLP+GK LSTQQSTTYDSN NDQSSNY DDGK T+PNDLCEIESSLC
Sbjct: 570 SGQKRKRSSQKTKKLPLGKILSTQQSTTYDSNANDQSSNYTDDGKETYPNDLCEIESSLC 629

Query: 540 AYDSDSDVVELERTISVSEAMTTRYVTVLMGTFLVEAVNLMLAEKQSCALIVDEENTLIG 599
           AYDSDS++VELER I VSEAMTTRYVTV M TFL+EAV+LMLAEKQSCALIVDEENTLIG
Sbjct: 630 AYDSDSEIVELERKICVSEAMTTRYVTVFMDTFLIEAVDLMLAEKQSCALIVDEENTLIG 689

Query: 600 ILALEDIQKLSKNAKSRSEQLKELVVSEICSLDGKICRVPWTATPSMDILTAKMIMKNLG 659
           ILALEDIQKLSKN  SR+EQLK LVVSEICSL+G+IC+VPWTATPSMDILTAKM+MKNLG
Sbjct: 690 ILALEDIQKLSKNVISRTEQLKGLVVSEICSLEGEICQVPWTATPSMDILTAKMVMKNLG 749

Query: 660 VNQVPVVKDQMGYLVGVLDWECIDLTCRILATRESLG 697
           V+QVPVVKDQMGYLVGVLD ECIDLT RILATRESLG
Sbjct: 750 VSQVPVVKDQMGYLVGVLDLECIDLTRRILATRESLG 770

BLAST of Spg001034 vs. TAIR 10
Match: AT4G35440.1 (chloride channel E )

HSP 1 Score: 709.1 bits (1829), Expect = 3.4e-204
Identity = 393/638 (61.60%), Postives = 471/638 (73.82%), Query Frame = 0

Query: 60  VHEIRDFSWDGIPNRGASWLREMPVEDIWKRVILVPACGGFLVSFLNLLRDATDVQVDRP 119
           VH +RDFSWDGIP+RGASWLRE P+   W RVILVP  GG +VS LN LR++        
Sbjct: 98  VHLLRDFSWDGIPDRGASWLREAPIGSNWLRVILVPTIGGLVVSILNQLRESAGKSTG-- 157

Query: 120 QDDGSSIKFGVPVSISNKFKAALQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGIS 179
            D  SS+         ++ KA L+PFLK VAA VTLGTGNSLGPEGPSV+IG S+ KG++
Sbjct: 158 -DSHSSL---------DRVKAVLRPFLKTVAACVTLGTGNSLGPEGPSVEIGASIAKGVN 217

Query: 180 TVFEKNSRTKLSLIAAGSAAGISSGCDSSIFSGFNAAVAGCFFAVESVLWPSPA-DSTLS 239
           ++F K+ +T  SL+AAGSAAGIS        SGFNAAVAGCFFAVESVLWPS + DS+ S
Sbjct: 218 SLFNKSPQTGFSLLAAGSAAGIS--------SGFNAAVAGCFFAVESVLWPSSSTDSSTS 277

Query: 240 LTNTTSMVILSAVIASVVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGVLCGLVSLGFS 299
           L NTTSMVILSAV ASVVS++GLG EPAFKVP+YDFRSP ELPLYLLLG LCGLVSL  S
Sbjct: 278 LPNTTSMVILSAVTASVVSEIGLGSEPAFKVPDYDFRSPGELPLYLLLGALCGLVSLALS 337

Query: 300 KCTSYMLGTVDKFHKDFSVPRALFPILGGFTTGLIALAYPEILYWGFENVDLLLESRPFV 359
           +CTS M   VD  +KD  +P+A+FP++GG + G+IAL YPE+LYWGF+NVD+LLE RPFV
Sbjct: 338 RCTSSMTSAVDSLNKDAGIPKAVFPVMGGLSVGIIALVYPEVLYWGFQNVDILLEKRPFV 397

Query: 360 KNLSAELLAQLVVIKVFATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNSV 419
           K LSA+LL QLV +K+ AT+ CRASGLVGGYYAPSLFIG A GMAYGKFIG+AL++ N  
Sbjct: 398 KGLSADLLLQLVAVKIAATAWCRASGLVGGYYAPSLFIGGAAGMAYGKFIGLALAQ-NPD 457

Query: 420 LDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWI 479
            + SI EVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVG+SSWI
Sbjct: 458 FNLSILEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGMSSWI 517

Query: 480 TSGQKRKRSSQNTKKLPIGKSLSTQQSTTYDSNENDQSSNYADDGKATFPNDLCEIESSL 539
           TSGQ +++ ++ TK+    KS    QS T   + +D+SS           N+LCE+ESSL
Sbjct: 518 TSGQSKRQETRETKETRKRKSQEAVQSLT---SSDDESST----------NNLCEVESSL 577

Query: 540 CAYDSDSDVVELERTISVSEAMTTRYVTVLMGTFLVEAVNLMLAEKQSCALIVDEENTLI 599
           C  DS +   EL ++I VSEAM TR+ TV+M T L EA+  ML EKQSCALIVD +N  +
Sbjct: 578 CLDDSLNQSEELPKSIFVSEAMRTRFATVMMSTSLEEALTRMLIEKQSCALIVDPDNIFL 637

Query: 600 GILALEDIQKLSKNAKSRSEQLKELVVSEICSLDGKICRVPWTATPSMDILTAKMIMKNL 659
           GIL L DIQ+ SK  K  + + K++ V++ICS  G  C+VPWT TP MD+L A+ IM   
Sbjct: 638 GILTLSDIQEFSKARKEGNNRPKDIFVNDICSRSGGKCKVPWTVTPDMDLLAAQTIMNKH 697

Query: 660 GVNQVPVVKDQMG----YLVGVLDWECIDLTCRILATR 693
            ++ V VV   +     + VGVLD ECI LT R LATR
Sbjct: 698 ELSHVAVVSGSIDAPRIHPVGVLDRECITLTRRALATR 701

BLAST of Spg001034 vs. TAIR 10
Match: AT4G35440.2 (chloride channel E )

HSP 1 Score: 702.2 bits (1811), Expect = 4.2e-202
Identity = 389/633 (61.45%), Postives = 467/633 (73.78%), Query Frame = 0

Query: 60  VHEIRDFSWDGIPNRGASWLREMPVEDIWKRVILVPACGGFLVSFLNLLRDATDVQVDRP 119
           VH +RDFSWDGIP+RGASWLRE P+   W RVILVP  GG +VS LN LR++        
Sbjct: 98  VHLLRDFSWDGIPDRGASWLREAPIGSNWLRVILVPTIGGLVVSILNQLRESAGKSTG-- 157

Query: 120 QDDGSSIKFGVPVSISNKFKAALQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGIS 179
            D  SS+         ++ KA L+PFLK VAA VTLGTGNSLGPEGPSV+IG S+ KG++
Sbjct: 158 -DSHSSL---------DRVKAVLRPFLKTVAACVTLGTGNSLGPEGPSVEIGASIAKGVN 217

Query: 180 TVFEKNSRTKLSLIAAGSAAGISSGCDSSIFSGFNAAVAGCFFAVESVLWPSPA-DSTLS 239
           ++F K+ +T  SL+AAGSAAGIS        SGFNAAVAGCFFAVESVLWPS + DS+ S
Sbjct: 218 SLFNKSPQTGFSLLAAGSAAGIS--------SGFNAAVAGCFFAVESVLWPSSSTDSSTS 277

Query: 240 LTNTTSMVILSAVIASVVSQVGLGVEPAFKVPEYDFRSPSELPLYLLLGVLCGLVSLGFS 299
           L NTTSMVILSAV ASVVS++GLG EPAFKVP+YDFRSP ELPLYLLLG LCGLVSL  S
Sbjct: 278 LPNTTSMVILSAVTASVVSEIGLGSEPAFKVPDYDFRSPGELPLYLLLGALCGLVSLALS 337

Query: 300 KCTSYMLGTVDKFHKDFSVPRALFPILGGFTTGLIALAYPEILYWGFENVDLLLESRPFV 359
           +CTS M   VD  +KD  +P+A+FP++GG + G+IAL YPE+LYWGF+NVD+LLE RPFV
Sbjct: 338 RCTSSMTSAVDSLNKDAGIPKAVFPVMGGLSVGIIALVYPEVLYWGFQNVDILLEKRPFV 397

Query: 360 KNLSAELLAQLVVIKVFATSLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNSV 419
           K LSA+LL QLV +K+ AT+ CRASGLVGGYYAPSLFIG A GMAYGKFIG+AL++ N  
Sbjct: 398 KGLSADLLLQLVAVKIAATAWCRASGLVGGYYAPSLFIGGAAGMAYGKFIGLALAQ-NPD 457

Query: 420 LDFSIFEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWI 479
            + SI EVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVG+SSWI
Sbjct: 458 FNLSILEVASPQAYGLVGMAATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGMSSWI 517

Query: 480 TSGQKRKRSSQNTKKLPIGKSLSTQQSTTYDSNENDQSSNYADDGKATFPNDLCEIESSL 539
           TSGQ +++ ++ TK+    KS    QS T   + +D+SS           N+LCE+ESSL
Sbjct: 518 TSGQSKRQETRETKETRKRKSQEAVQSLT---SSDDESST----------NNLCEVESSL 577

Query: 540 CAYDSDSDVVELERTISVSEAMTTRYVTVLMGTFLVEAVNLMLAEKQSCALIVDEENTLI 599
           C  DS +   EL ++I VSEAM TR+ TV+M T L EA+  ML EKQSCALIVD +N  +
Sbjct: 578 CLDDSLNQSEELPKSIFVSEAMRTRFATVMMSTSLEEALTRMLIEKQSCALIVDPDNIFL 637

Query: 600 GILALEDIQKLSKNAKSRSEQLKELVVSEICSLDGKICRVPWTATPSMDILTAKMIMKNL 659
           GIL L DIQ+ SK  K  + + K++ V++ICS  G  C+VPWT TP MD+L A+ IM   
Sbjct: 638 GILTLSDIQEFSKARKEGNNRPKDIFVNDICSRSGGKCKVPWTVTPDMDLLAAQTIMNKH 696

Query: 660 GVNQVPVVKDQMG----YLVGVLDWECIDLTCR 688
            ++ V VV   +     + VGVLD ECI LT R
Sbjct: 698 ELSHVAVVSGSIDAPRIHPVGVLDRECITLTRR 696

BLAST of Spg001034 vs. TAIR 10
Match: AT1G55620.2 (chloride channel F )

HSP 1 Score: 401.0 bits (1029), Expect = 2.0e-111
Identity = 267/646 (41.33%), Postives = 375/646 (58.05%), Query Frame = 0

Query: 28  QFLPQMAPPTLSLMDSKIVKWVAAAMAV--YWVFVHEIRDFSWDGIPNRGASWLREMPVE 87
           + L   APP  +L+    +  VAA + V  +   VH I +++W G PN GA+WLR   + 
Sbjct: 119 EVLGDSAPPEWALLLIGCLIGVAAGICVAGFNKGVHVIHEWAWAGTPNEGAAWLRLQRLA 178

Query: 88  DIWKRVILVPACGGFLVSFLNLLRDATDVQVDRPQDDGSSIKFGVPVSISNKFKAALQPF 147
           D W R++L+P  GG +V  ++ L +     +D+ +   SS + G+       F A + P 
Sbjct: 179 DTWHRILLIPVTGGVIVGMMHGLLEI----LDQIRQSNSSQRQGL------DFLAGIYPV 238

Query: 148 LKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGISTVFEKNSRTKLSLIAAGSAAGISSGC 207
           +KA+ A+VTLGTG SLGPEGPSVDIG S   G + + E N   +++L AAG+A+GI+   
Sbjct: 239 IKAIQAAVTLGTGCSLGPEGPSVDIGKSCANGFALMMENNRERRIALTAAGAASGIA--- 298

Query: 208 DSSIFSGFNAAVAGCFFAVESVLWPSPADSTLSLTNTTSMVILSAVIASVVSQVGLGVEP 267
                SGFNAAVAGCFFA+E+VL P  A++  S   TT+M+IL++VI+S VS   LG + 
Sbjct: 299 -----SGFNAAVAGCFFAIETVLRPLRAEN--SPPFTTAMIILASVISSTVSNALLGTQS 358

Query: 268 AFKVPEYDFRSPSELPLYLLLGVLCGLVSLGFSKCTSYMLGTVDKFHKDFSVPRALFPIL 327
           AF VP YD +S +ELPLYL+LG+LCG VS+ FS+  ++   + D     F +P  + P L
Sbjct: 359 AFTVPSYDLKSAAELPLYLILGMLCGAVSVVFSRLVTWFTKSFDFIKDKFGLPAIVCPAL 418

Query: 328 GGFTTGLIALAYPEILYWGFENVDLLLESRPFVKNLSAELLAQLVVIKVFATSLCRASGL 387
           GG   G+IAL YP ILYWGF NV+ +L +          LLAQL   KV AT+LC+ SGL
Sbjct: 419 GGLGAGIIALKYPGILYWGFTNVEEILHTGKSASAPGIWLLAQLAAAKVVATALCKGSGL 478

Query: 388 VGGYYAPSLFIGAATGMAYGKFIGIALSEPNSVLDFSIFEVASPQAYGLVGMAATLAGVC 447
           VGG YAPSL IGAA G  +G   G A    N  +  +   VA PQAY LVGMAATLA +C
Sbjct: 479 VGGLYAPSLMIGAAVGAVFG---GSAAEIINRAIPGNA-AVAQPQAYALVGMAATLASMC 538

Query: 448 QVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWITSGQKRKRSSQNTKKLPIGKSLSTQQS 507
            VPLT+VLLLFELT+DYRI+LPL+GAVG++ W+ S   + + S +++    G+  S+   
Sbjct: 539 SVPLTSVLLLFELTKDYRILLPLMGAVGLAIWVPSVANQGKESDSSEGRSTGRGYSSLSP 598

Query: 508 TTYDSNENDQSSNYADDGKATFPNDLCEIESSLCAYDSDSDVVELERTISVSEAMTTRYV 567
           +   +    + ++ AD        +L  IE+    ++S  D   +   + V   M+  YV
Sbjct: 599 SERKTEGVWRHTDNADS------LELTVIENP--DHNSFLDEETILEDLKVMRVMSKNYV 658

Query: 568 TVLMGTFLVEAVNLMLAEKQSCALIVDEENTLIGILALEDIQKLSKNAKSRSEQLKELVV 627
            V  GT L EA N++    Q+C ++VD+++ L GIL   DI++   N  S         V
Sbjct: 659 KVSSGTTLREARNILKESHQNCIMVVDDDDFLAGILTHGDIRRYLSNNASTILDENTCPV 718

Query: 628 SEIC----SLDGKICRVPWTATPSMDILTAKMIMKNLGVNQVPVVK 668
           S +C    S  G+  R   T  P   +  AK +M+  GV Q+PVVK
Sbjct: 719 SSVCTKKISYRGQE-RGLLTCYPDATVGVAKELMEARGVKQLPVVK 731

BLAST of Spg001034 vs. TAIR 10
Match: AT1G55620.1 (chloride channel F )

HSP 1 Score: 351.3 bits (900), Expect = 1.8e-96
Identity = 233/534 (43.63%), Postives = 318/534 (59.55%), Query Frame = 0

Query: 138 FKAALQPFLKAVAASVTLGTGNSLGPEGPSVDIGTSVGKGISTVFEKNSRTKLSLIAAGS 197
           F A + P +KA+ A+VTLGTG SLGPEGPSVDIG S   G + + E N   +++L AAG+
Sbjct: 25  FLAGIYPVIKAIQAAVTLGTGCSLGPEGPSVDIGKSCANGFALMMENNRERRIALTAAGA 84

Query: 198 AAGISSGCDSSIFSGFNAAVAGCFFAVESVLWPSPADSTLSLTNTTSMVILSAVIASVVS 257
           A+GI+        SGFNAAVAGCFFA+E+VL P  A++  S   TT+M+IL++VI+S VS
Sbjct: 85  ASGIA--------SGFNAAVAGCFFAIETVLRPLRAEN--SPPFTTAMIILASVISSTVS 144

Query: 258 QVGLGVEPAFKVPEYDFRSPSELPLYLLLGVLCGLVSLGFSKCTSYMLGTVDKFHKDFSV 317
              LG + AF VP YD +S +ELPLYL+LG+LCG VS+ FS+  ++   + D     F +
Sbjct: 145 NALLGTQSAFTVPSYDLKSAAELPLYLILGMLCGAVSVVFSRLVTWFTKSFDFIKDKFGL 204

Query: 318 PRALFPILGGFTTGLIALAYPEILYWGFENVDLLLESRPFVKNLSAELLAQLVVIKVFAT 377
           P  + P LGG   G+IAL YP ILYWGF NV+ +L +          LLAQL   KV AT
Sbjct: 205 PAIVCPALGGLGAGIIALKYPGILYWGFTNVEEILHTGKSASAPGIWLLAQLAAAKVVAT 264

Query: 378 SLCRASGLVGGYYAPSLFIGAATGMAYGKFIGIALSEPNSVLDFSIFEVASPQAYGLVGM 437
           +LC+ SGLVGG YAPSL IGAA G  +G   G A    N  +  +   VA PQAY LVGM
Sbjct: 265 ALCKGSGLVGGLYAPSLMIGAAVGAVFG---GSAAEIINRAIPGNA-AVAQPQAYALVGM 324

Query: 438 AATLAGVCQVPLTAVLLLFELTQDYRIVLPLLGAVGVSSWITSGQKRKRSSQNTKKLPIG 497
           AATLA +C VPLT+VLLLFELT+DYRI+LPL+GAVG++ W+ S   + + S +++    G
Sbjct: 325 AATLASMCSVPLTSVLLLFELTKDYRILLPLMGAVGLAIWVPSVANQGKESDSSEGRSTG 384

Query: 498 KSLSTQQSTTYDSNENDQSSNYADDGKATFPNDLCEIESSLCAYDSDSDVVELERTISVS 557
           +  S+   +   +    + ++ AD        +L  IE+    ++S  D   +   + V 
Sbjct: 385 RGYSSLSPSERKTEGVWRHTDNADS------LELTVIENP--DHNSFLDEETILEDLKVM 444

Query: 558 EAMTTRYVTVLMGTFLVEAVNLMLAEKQSCALIVDEENTLIGILALEDIQKLSKNAKSRS 617
             M+  YV V  GT L EA N++    Q+C ++VD+++ L GIL   DI++   N  S  
Sbjct: 445 RVMSKNYVKVSSGTTLREARNILKESHQNCIMVVDDDDFLAGILTHGDIRRYLSNNASTI 504

Query: 618 EQLKELVVSEIC----SLDGKICRVPWTATPSMDILTAKMIMKNLGVNQVPVVK 668
                  VS +C    S  G+  R   T  P   +  AK +M+  GV Q+PVVK
Sbjct: 505 LDENTCPVSSVCTKKISYRGQE-RGLLTCYPDATVGVAKELMEARGVKQLPVVK 535