Spg000942 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg000942
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionN-acyl-L-amino-acid amidohydrolase
Locationscaffold8: 39923929 .. 39927054 (-)
RNA-Seq ExpressionSpg000942
SyntenySpg000942
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTCTGATCATTCGAATCTCTCCTGAACTTTGTTAGTCTTGGAAATTGTGAGGAATAAGCACCACTAAACCATGAATTTGTGAGTCTCTCTGTACTTTCTTCAGTTGCAGTCTACTAAGCACCAACTGAACCATGAATTTCAAATCTATTATAACCCTTTTTTTGTTTCTCTCAATTTCCACTGCTGAAGAAGACCCATCTTCTTCCTCTTCAATCATATCAAGGTTCCAACAATACCTTCAAATTAACACCGTTCAACCCAATCCCCAGTACTATGAAGCTGCCGATTTCATCATCTCTCAGGCTAAATCGCTTTCCCTTGAATCCCAAACAATCGAGTTCGTCGAGGGCAAGCCTCTAATCCTCCTCAAATGGCCTGGTTCGAACCCCGATCTTCCTTCCATCCTTCTCAATTCCCACACCGATGTTGTTCCAGCTGAGCATCACAAATGGACGCACCCTCCTCTTGGAGCTCATATCGATTCCCATGGCAATATCTATGCCAGAGGGTCTCAGGATATGAAGTGCGTTGGGATGCAGTACCTCGAGGCGATTCGGCGTTTGAAGGCATCTGGGTTCCAGCCTGTGCGCTCTGTCTACCTTTCTTTTGTCCCGGATGAAGAAATTGGTGGCCATGGTGGTGCTGAGAAGTTTGCTGAATCTGATGAGTTCAAGAAATTGAATGTCGCCATTGTGCTCGATGAAGGTTTTGAATGAAACCCTTTTGCTGATTTTCTGATGGGTGTGTTCTTTTTTATCCTTGTTCTTCAAATGTTTAGACTAATGGAGTTTCGAATCATCAGGGTTACCTTCTTCGGGTGAGAATTACAGGGTGTTTTATGGGGAGAGGAGCCCTTGGTGGTTGGTGATCAAGGCAGTGGGGGCGCCAGGCCATGGAGCTAAGCTTTATGATAACACTGCCTTGGAGAATCTGTTCAAAAGTATTGAGAGTGTGAGAAGATTCAGAGCTTCACAGTTTGATTTGTTGAAGGCTGGTTTGAAGGCTGAAGGAGAGGTGGTCTCTGTCAACATGGTTTTCTTAAAATCTGGCACTCCATCTCCTACTGTGAGTTGATCTTTGAAACTTGTTTCTCCATGAATGCTTGAAGTTGTAATTCTTCTGAACGTTTAATCCGGAGTAGTAAACTGTATATAGATGAAACTAGAGAAGACTGTATTTACACGAACTTACTTGAACAGGATCTGTTGTATAGGTTGGACAACTGTGTCCTTGAGTATTTGTTTAATCACTTCATCTAGCAAATTAGCTAGAATGTATTTAGTATTGGTCTATTCTTTGTTTGAGTTTCAGGTGGAACATCAAAATCAATGGTTTATATTTAGACAGTTGAAATAATAGCAAATTGGGTGCTTATATCAATCAAGAATGTTGAGTTGAATTCTTGATGTTAGTGTCATTATGCTTTGTCAGGAGAGACTATTTTTGTTGAAATTCTGCGTTATATGGTACAATGGGTGTGATTAGCTCTGCCATGTTAAAGAATCTTTAGGCTGATCAAACTCTGTTGCTAATATAACCTGATGGGTACTCTGTTATATCTACCAGGGCTTTGTCATGAATCTGCAGCCATCTGAAGCCGAGGCTGGCTTTGACGTTCGCCTCCCACCAACTGCGAATCCAGAGTCTCTTGAGAGACGAATTGCAGAGGAATGGGCACCTGTTTCTCGTAATATGACATTCGAGGTAGCAATTTTGGAGTCTTTAGATTCTGACCTAAGAAGCATGGGATACAGTCACAGCATGTTATTTCCTAAAAGATGAAGACACAAATAAGGGTCATGGGATGAACAAATGTACTTTGAATATATCTAATTTTTGCGTCATAAATTAGTTCGTCTATGATTGAAGGGATGAAATATGTTACAACTTACAACAAGTATAGAACATGTGTCTAACAAGTGTTGGAGTGATAAATGTCATATAGATGAAACTAAAATGTCCGTGCTTGGAAGTTTCTAGCCAACTCAGAAAATTGGATACCGGATAAAATTTTAGGGTACCATCACTTATACTGACTATGCTGATTACTGAATTCCAGAATTCAATCTTTTTAACCATTTAAATTCTTCCTCTCACATCTCTCCTTGTGCTTGATCGTTGCTCAGTTTAAGCAGAAGGAGTCTGTTCTCGACAAGTTTGGAAAACCAGCTCTCACTGCTATTGACAGCTCTAACCCATGGTGGAATCTTCTTGAAGAAGCTGTAAGAAATGCTAATGGGAAACTTGGAAACCCAGAAATCTTTCCCGCTTCAACCGATGCCCGGTACTTTCGAAATCAAGGCCTCCCTGCAATTGGCTTCTCTCCAATGGCTAACACTCCTATTCTTCTTCATGACCATAATGAGGTGAGAGTCTTATCAGTTATATCTACTAATCTGGTTGTATGATATGGTGTCTTGTTCAGGTAGAAACTTTTAGGTTAGAAATAGCATAAATTTTGGCCTTAGTTTGCCTTTGCCAACCTTCAACCTATGTCGAAATCTTGATTGATCATATTCATTTTGAACATCTGCCTAGTAGTAAATTGATAATGAGCAGTTGAGAACTCTAGAAGCAATCGTAATTGCTTTACATGCTGCGGTACGATCTCGCTAAACCGCTCCATTTTAGCGAGATTTTCATTTTTTGTTTCTGGTCTAGATGGTTGTTTTTCTGGCTGACATTCAACCACATTGTTATCCAAACCATTAATATCTTCACCTGTTTCAGTTTTTGAACCAAGCCGAGTATTTGAAAGGCATCGAGGTGTACGAATCAATAATCAAGGTGTATGCTTCTTACGACGGTCAGAAACCGACCAAAAGCTTCAAAGATGAGTTATAAGAAGGCAGCAAAAGCGGTGTTATCCATCCAGCATTCGACGGCGTCGTTCGAAATTGCTGTATAGTTTTGCAGCATGGGATTGGAAGGGAAGGAATCTGATTCTGTTCCATAAATAAGGTCGATCCTTGTAACTTGGTGATAATCTTCTGGACCTTTACATCATTGAACTTTGGTTGTGGTATAAATTTCAGGAAACAGAATTTGGTATCAAATAAAGTAAAAACAGAATTTGCTTTTGAATAAATTCTCGAGTTACTGACGAATTGACAATTATGAACAA

mRNA sequence

ATGAATTTCAAATCTATTATAACCCTTTTTTTGTTTCTCTCAATTTCCACTGCTGAAGAAGACCCATCTTCTTCCTCTTCAATCATATCAAGGTTCCAACAATACCTTCAAATTAACACCGTTCAACCCAATCCCCAGTACTATGAAGCTGCCGATTTCATCATCTCTCAGGCTAAATCGCTTTCCCTTGAATCCCAAACAATCGAGTTCGTCGAGGGCAAGCCTCTAATCCTCCTCAAATGGCCTGGTTCGAACCCCGATCTTCCTTCCATCCTTCTCAATTCCCACACCGATGTTGTTCCAGCTGAGCATCACAAATGGACGCACCCTCCTCTTGGAGCTCATATCGATTCCCATGGCAATATCTATGCCAGAGGGTCTCAGGATATGAAGTGCGTTGGGATGCAGTACCTCGAGGCGATTCGGCGTTTGAAGGCATCTGGGTTCCAGCCTGTGCGCTCTGTCTACCTTTCTTTTGTCCCGGATGAAGAAATTGGTGGCCATGGTGGTGCTGAGAAGTTTGCTGAATCTGATGAGTTCAAGAAATTGAATGTCGCCATTGTGCTCGATGAAGGGTTACCTTCTTCGGGTGAGAATTACAGGGTGTTTTATGGGGAGAGGAGCCCTTGGTGGTTGGTGATCAAGGCAGTGGGGGCGCCAGGCCATGGAGCTAAGCTTTATGATAACACTGCCTTGGAGAATCTGTTCAAAAGTATTGAGAGTGTGAGAAGATTCAGAGCTTCACAGTTTGATTTGTTGAAGGCTGGTTTGAAGGCTGAAGGAGAGGTGGTCTCTGTCAACATGGTTTTCTTAAAATCTGGCACTCCATCTCCTACTGGCTTTGTCATGAATCTGCAGCCATCTGAAGCCGAGGCTGGCTTTGACGTTCGCCTCCCACCAACTGCGAATCCAGAGTCTCTTGAGAGACGAATTGCAGAGGAATGGGCACCTGTTTCTCGTAATATGACATTCGAGTTTAAGCAGAAGGAGTCTGTTCTCGACAAGTTTGGAAAACCAGCTCTCACTGCTATTGACAGCTCTAACCCATGGTGGAATCTTCTTGAAGAAGCTGTAAGAAATGCTAATGGGAAACTTGGAAACCCAGAAATCTTTCCCGCTTCAACCGATGCCCGGTACTTTCGAAATCAAGGCCTCCCTGCAATTGGCTTCTCTCCAATGGCTAACACTCCTATTCTTCTTCATGACCATAATGAGTTTTTGAACCAAGCCGAGTATTTGAAAGGCATCGAGGTGTACGAATCAATAATCAAGGTGTATGCTTCTTACGACGGTCAGAAACCGACCAAAAGCTTCAAAGATGAGTTATAA

Coding sequence (CDS)

ATGAATTTCAAATCTATTATAACCCTTTTTTTGTTTCTCTCAATTTCCACTGCTGAAGAAGACCCATCTTCTTCCTCTTCAATCATATCAAGGTTCCAACAATACCTTCAAATTAACACCGTTCAACCCAATCCCCAGTACTATGAAGCTGCCGATTTCATCATCTCTCAGGCTAAATCGCTTTCCCTTGAATCCCAAACAATCGAGTTCGTCGAGGGCAAGCCTCTAATCCTCCTCAAATGGCCTGGTTCGAACCCCGATCTTCCTTCCATCCTTCTCAATTCCCACACCGATGTTGTTCCAGCTGAGCATCACAAATGGACGCACCCTCCTCTTGGAGCTCATATCGATTCCCATGGCAATATCTATGCCAGAGGGTCTCAGGATATGAAGTGCGTTGGGATGCAGTACCTCGAGGCGATTCGGCGTTTGAAGGCATCTGGGTTCCAGCCTGTGCGCTCTGTCTACCTTTCTTTTGTCCCGGATGAAGAAATTGGTGGCCATGGTGGTGCTGAGAAGTTTGCTGAATCTGATGAGTTCAAGAAATTGAATGTCGCCATTGTGCTCGATGAAGGGTTACCTTCTTCGGGTGAGAATTACAGGGTGTTTTATGGGGAGAGGAGCCCTTGGTGGTTGGTGATCAAGGCAGTGGGGGCGCCAGGCCATGGAGCTAAGCTTTATGATAACACTGCCTTGGAGAATCTGTTCAAAAGTATTGAGAGTGTGAGAAGATTCAGAGCTTCACAGTTTGATTTGTTGAAGGCTGGTTTGAAGGCTGAAGGAGAGGTGGTCTCTGTCAACATGGTTTTCTTAAAATCTGGCACTCCATCTCCTACTGGCTTTGTCATGAATCTGCAGCCATCTGAAGCCGAGGCTGGCTTTGACGTTCGCCTCCCACCAACTGCGAATCCAGAGTCTCTTGAGAGACGAATTGCAGAGGAATGGGCACCTGTTTCTCGTAATATGACATTCGAGTTTAAGCAGAAGGAGTCTGTTCTCGACAAGTTTGGAAAACCAGCTCTCACTGCTATTGACAGCTCTAACCCATGGTGGAATCTTCTTGAAGAAGCTGTAAGAAATGCTAATGGGAAACTTGGAAACCCAGAAATCTTTCCCGCTTCAACCGATGCCCGGTACTTTCGAAATCAAGGCCTCCCTGCAATTGGCTTCTCTCCAATGGCTAACACTCCTATTCTTCTTCATGACCATAATGAGTTTTTGAACCAAGCCGAGTATTTGAAAGGCATCGAGGTGTACGAATCAATAATCAAGGTGTATGCTTCTTACGACGGTCAGAAACCGACCAAAAGCTTCAAAGATGAGTTATAA

Protein sequence

MNFKSIITLFLFLSISTAEEDPSSSSSIISRFQQYLQINTVQPNPQYYEAADFIISQAKSLSLESQTIEFVEGKPLILLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPVRSVYLSFVPDEEIGGHGGAEKFAESDEFKKLNVAIVLDEGLPSSGENYRVFYGERSPWWLVIKAVGAPGHGAKLYDNTALENLFKSIESVRRFRASQFDLLKAGLKAEGEVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGFDVRLPPTANPESLERRIAEEWAPVSRNMTFEFKQKESVLDKFGKPALTAIDSSNPWWNLLEEAVRNANGKLGNPEIFPASTDARYFRNQGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVYESIIKVYASYDGQKPTKSFKDEL
Homology
BLAST of Spg000942 vs. NCBI nr
Match: XP_038876663.1 (aminoacylase-1 [Benincasa hispida])

HSP 1 Score: 841.3 bits (2172), Expect = 3.9e-240
Identity = 407/438 (92.92%), Postives = 422/438 (96.35%), Query Frame = 0

Query: 5   SIITLFLFLSISTAEEDPSSSSSIISRFQQYLQINTVQPNPQYYEAADFIISQAKSLSLE 64
           S ITLFL LS+ST+EEDPSS+S+IISRFQQYLQINTVQP+PQYYEAADFIISQAK+LSLE
Sbjct: 11  SFITLFLLLSVSTSEEDPSSTSAIISRFQQYLQINTVQPSPQYYEAADFIISQAKALSLE 70

Query: 65  SQTIEFVEGKPLILLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNIYA 124
           SQTIEFVEGKPLILLKWPG NPDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNIYA
Sbjct: 71  SQTIEFVEGKPLILLKWPGLNPDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNIYA 130

Query: 125 RGSQDMKCVGMQYLEAIRRLKASGFQPVRSVYLSFVPDEEIGGHGGAEKFAESDEFKKLN 184
           RGSQDMKCVGMQYLEAIRRLKASGF+P+RSVYLSFVPDEEIGGHGGAEKFAESD F KLN
Sbjct: 131 RGSQDMKCVGMQYLEAIRRLKASGFEPLRSVYLSFVPDEEIGGHGGAEKFAESDVFNKLN 190

Query: 185 VAIVLDEGLPSSGENYRVFYGERSPWWLVIKAVGAPGHGAKLYDNTALENLFKSIESVRR 244
           VAIVLDEGLPS GENYRVFYGE+SPWWLVIKAVGAPGHGAKLYDN ALENLFKSIES+RR
Sbjct: 191 VAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGAKLYDNAALENLFKSIESIRR 250

Query: 245 FRASQFDLLKAGLKAEGEVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGFDVRLPPTANP 304
           FRASQFDL+KAGLK EG+VVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGFDVRLPPTANP
Sbjct: 251 FRASQFDLIKAGLKTEGDVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGFDVRLPPTANP 310

Query: 305 ESLERRIAEEWAPVSRNMTFEFKQKESVLDKFGKPALTAIDSSNPWWNLLEEAVRNANGK 364
           ESLERRIAEEWAP SRNMTFEFKQKESV +KFGKPALTAID SNPWWNLLEEAVRNANGK
Sbjct: 311 ESLERRIAEEWAPASRNMTFEFKQKESVYNKFGKPALTAIDKSNPWWNLLEEAVRNANGK 370

Query: 365 LGNPEIFPASTDARYFRNQGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVYESIIK 424
           L NPEIFPASTDARYFRN GLPAIGFSPM NTPILLHDHNEFLNQAEYLKGIEVY+SIIK
Sbjct: 371 LANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLKGIEVYKSIIK 430

Query: 425 VYASYDGQKPTKSFKDEL 443
            YASYDG KPT+SFKDEL
Sbjct: 431 AYASYDGDKPTESFKDEL 448

BLAST of Spg000942 vs. NCBI nr
Match: KAA0047810.1 (aminoacylase-1 [Cucumis melo var. makuwa])

HSP 1 Score: 839.7 bits (2168), Expect = 1.1e-239
Identity = 410/448 (91.52%), Postives = 424/448 (94.64%), Query Frame = 0

Query: 1   MNFK------SIITLFLFLSISTAEEDPSSSSSIISRFQQYLQINTVQPNPQYYEAADFI 60
           MNFK      SIITLF FLSIST+E+DPSS S+I+SRFQQYLQINTVQP+PQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFI 60

Query: 61  ISQAKSLSLESQTIEFVEGKPLILLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGA 120
           ISQAKSLSLES TIEFVEGKPLI+LKWPGSN DLPSILLNSHTDVVPAEHHKWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPVRSVYLSFVPDEEIGGHGGAEKF 180
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQP+RSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 181 AESDEFKKLNVAIVLDEGLPSSGENYRVFYGERSPWWLVIKAVGAPGHGAKLYDNTALEN 240
           AESDEFKKLNVAIVLDEGLPS GENYRVFYGE+SPWWLVIKAVGAPGHGA+LYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 241 LFKSIESVRRFRASQFDLLKAGLKAEGEVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF 300
           LFKSIE+VRRFRASQFDL+KAGLK EG+VVSVNMVFLKSG PSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 301 DVRLPPTANPESLERRIAEEWAPVSRNMTFEFKQKESVLDKFGKPALTAIDSSNPWWNLL 360
           DVRLPPT NPESLERRIAEEWAP SRNMTFEFKQKES+ DKFGKPALTAID SNPWWNLL
Sbjct: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360

Query: 361 EEAVRNANGKLGNPEIFPASTDARYFRNQGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 420
           EEAVRNANGKL NPEIFPASTDARYFRN GLPAIGFSPM NTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 421 GIEVYESIIKVYASYDGQKPTKSFKDEL 443
           GIEVYESIIK YASYDG K  +SFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 448

BLAST of Spg000942 vs. NCBI nr
Match: XP_008448158.1 (PREDICTED: aminoacylase-1 [Cucumis melo] >TYK01049.1 aminoacylase-1 [Cucumis melo var. makuwa])

HSP 1 Score: 838.2 bits (2164), Expect = 3.3e-239
Identity = 410/448 (91.52%), Postives = 424/448 (94.64%), Query Frame = 0

Query: 1   MNFK------SIITLFLFLSISTAEEDPSSSSSIISRFQQYLQINTVQPNPQYYEAADFI 60
           MNFK      SIITLF FLSIST+E++PSS S+IISRFQQYLQINTVQP+PQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQNPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 61  ISQAKSLSLESQTIEFVEGKPLILLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGA 120
           ISQAKSLSLES TIEFVEGKPLI+LKWPGSN DLPSILLNSHTDVVPAEHHKWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPVRSVYLSFVPDEEIGGHGGAEKF 180
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQP+RSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 181 AESDEFKKLNVAIVLDEGLPSSGENYRVFYGERSPWWLVIKAVGAPGHGAKLYDNTALEN 240
           AESDEFKKLNVAIVLDEGLPS GENYRVFYGE+SPWWLVIKAVGAPGHGA+LYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 241 LFKSIESVRRFRASQFDLLKAGLKAEGEVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF 300
           LFKSIE+VRRFRASQFDL+KAGLK EG+VVSVNMVFLKSG PSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 301 DVRLPPTANPESLERRIAEEWAPVSRNMTFEFKQKESVLDKFGKPALTAIDSSNPWWNLL 360
           DVRLPPT NPESLERRIAEEWAP SRNMTFEFKQKES+ DKFGKPALTAID SNPWWNLL
Sbjct: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360

Query: 361 EEAVRNANGKLGNPEIFPASTDARYFRNQGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 420
           EEAVRNANGKL NPEIFPASTDARYFRN GLPAIGFSPM NTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 421 GIEVYESIIKVYASYDGQKPTKSFKDEL 443
           GIEVYESIIK YASYDG K  +SFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 448

BLAST of Spg000942 vs. NCBI nr
Match: XP_004139978.1 (aminoacylase-1 [Cucumis sativus] >KGN46643.1 hypothetical protein Csa_005514 [Cucumis sativus])

HSP 1 Score: 833.6 bits (2152), Expect = 8.2e-238
Identity = 407/448 (90.85%), Postives = 424/448 (94.64%), Query Frame = 0

Query: 1   MNFK------SIITLFLFLSISTAEEDPSSSSSIISRFQQYLQINTVQPNPQYYEAADFI 60
           MNFK      SIITLF FLSIST+E+DPSS S+IISRFQQYLQINTVQP+PQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 61  ISQAKSLSLESQTIEFVEGKPLILLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGA 120
           ISQAKSLSLES TIEFVEGKPL++LKWPGSNP+LPSILLNSHTDVVPAEH KWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLVILKWPGSNPELPSILLNSHTDVVPAEHKKWTHPPLGA 120

Query: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPVRSVYLSFVPDEEIGGHGGAEKF 180
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQP+RSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 181 AESDEFKKLNVAIVLDEGLPSSGENYRVFYGERSPWWLVIKAVGAPGHGAKLYDNTALEN 240
           AESDEFKKLNVAIVLDEGLPS GENYRVFYGE+SPWWLVIKAVGAPGHGA+LYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 241 LFKSIESVRRFRASQFDLLKAGLKAEGEVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF 300
           LFKSIE+VRRFRASQFDL+KAGLK EG+VVSVNMVFLKSG PSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 301 DVRLPPTANPESLERRIAEEWAPVSRNMTFEFKQKESVLDKFGKPALTAIDSSNPWWNLL 360
           DVR+PPT NPESLERRIAEEWAP SRNMTFEFKQKES+ +KFGKPALTAID SNP WNLL
Sbjct: 301 DVRVPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPRWNLL 360

Query: 361 EEAVRNANGKLGNPEIFPASTDARYFRNQGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 420
           EEAVRNANGKL NPEIFPASTDARYFRN GLPAIGFSPM NTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 421 GIEVYESIIKVYASYDGQKPTKSFKDEL 443
           GIEVYESIIK YASYDG KP +SFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKPMESFKDEL 448

BLAST of Spg000942 vs. NCBI nr
Match: XP_023532856.1 (aminoacylase-1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 831.2 bits (2146), Expect = 4.0e-237
Identity = 412/449 (91.76%), Postives = 423/449 (94.21%), Query Frame = 0

Query: 1   MNFK-------SIITLFLFLSISTAEEDPSSSSSIISRFQQYLQINTVQPNPQYYEAADF 60
           MNFK       SI TLFLFLSIS AEEDP SSSSI+SRFQ+YLQINTVQPNPQYYEAADF
Sbjct: 1   MNFKSLNHLLLSITTLFLFLSISAAEEDPLSSSSIVSRFQRYLQINTVQPNPQYYEAADF 60

Query: 61  IISQAKSLSLESQTIEFVEGKPLILLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLG 120
           IISQAKSLSLE  T+EFV GKPL+LLKWPGSNP+LPSILLNSHTDVVP EHHKWTHPPLG
Sbjct: 61  IISQAKSLSLEYHTVEFVVGKPLVLLKWPGSNPNLPSILLNSHTDVVPFEHHKWTHPPLG 120

Query: 121 AHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPVRSVYLSFVPDEEIGGHGGAEK 180
           AHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGF+PVRSVYLSFVPDEEIGGHGGAEK
Sbjct: 121 AHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFEPVRSVYLSFVPDEEIGGHGGAEK 180

Query: 181 FAESDEFKKLNVAIVLDEGLPSSGENYRVFYGERSPWWLVIKAVGAPGHGAKLYDNTALE 240
           FAESDEFKKLNVAIVLDEGLPS GENYR FYGERSPWWLVIKAVGAPGHGAKLYDN ALE
Sbjct: 181 FAESDEFKKLNVAIVLDEGLPSPGENYRAFYGERSPWWLVIKAVGAPGHGAKLYDNAALE 240

Query: 241 NLFKSIESVRRFRASQFDLLKAGLKAEGEVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAG 300
           NLFKSIESVRRFRASQFDLLKAGLKAEGEVVSVNMV LK+GTPSPTGFVMNLQPSEAEAG
Sbjct: 241 NLFKSIESVRRFRASQFDLLKAGLKAEGEVVSVNMVSLKAGTPSPTGFVMNLQPSEAEAG 300

Query: 301 FDVRLPPTANPESLERRIAEEWAPVSRNMTFEFKQKESVLDKFGKPALTAIDSSNPWWNL 360
           FDVRLPPTA+PESLERRIAEEWAPVSRNMTFEFKQKES+ DKFGKPALTAIDSSNPWWNL
Sbjct: 301 FDVRLPPTADPESLERRIAEEWAPVSRNMTFEFKQKESIYDKFGKPALTAIDSSNPWWNL 360

Query: 361 LEEAVRNANGKLGNPEIFPASTDARYFRNQGLPAIGFSPMANTPILLHDHNEFLNQAEYL 420
           LEEAVRNANGKLGNPEIFPASTDARYFRNQGLPAIGFSPMANTP LLHDHNEFLNQAEYL
Sbjct: 361 LEEAVRNANGKLGNPEIFPASTDARYFRNQGLPAIGFSPMANTPSLLHDHNEFLNQAEYL 420

Query: 421 KGIEVYESIIKVYASYDGQKPTKSFKDEL 443
           KGIEVYESIIK YASY G+    +FKDEL
Sbjct: 421 KGIEVYESIIKSYASYVGR---DNFKDEL 446

BLAST of Spg000942 vs. ExPASy Swiss-Prot
Match: Q99JW2 (Aminoacylase-1 OS=Mus musculus OX=10090 GN=Acy1 PE=1 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 7.5e-101
Identity = 187/411 (45.50%), Postives = 253/411 (61.56%), Query Frame = 0

Query: 20  EDPSSSSSIISRFQQYLQINTVQPNPQYYEAADFIISQAKSLSLESQTIEFVEGKPLILL 79
           +DP S    ++ F+QYL+I TVQPNP Y  A  F+  +A+ L L  Q IE V G  + +L
Sbjct: 4   KDPESEHPSVTLFRQYLRICTVQPNPDYGGAITFLEERARQLGLSCQKIEVVPGFVITVL 63

Query: 80  KWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLE 139
            WPG+NP LPSILLNSHTDVVP     W H P  A  DS G IYARGSQDMK V +QYLE
Sbjct: 64  TWPGTNPSLPSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGSQDMKSVSIQYLE 123

Query: 140 AIRRLKASGFQPVRSVYLSFVPDEEIGGHGGAEKFAESDEFKKLNVAIVLDEGLPSSGEN 199
           A+RRLK+ G +  R+++++FVPDEE+GGH G E F +  EF+ L     LDEGL +  + 
Sbjct: 124 AVRRLKSEGHRFPRTIHMTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDA 183

Query: 200 YRVFYGERSPWWLVIKAVGAPGHGAKLYDNTALENLFKSIESVRRFRASQFDLLKAG--L 259
           + VFY ERSPWW+ + + G PGH ++  ++TA E L K I S+  FR  +   L+A   L
Sbjct: 184 FTVFYSERSPWWVRVTSTGKPGHASRFIEDTAAEKLHKVISSILAFREKERQRLQANPHL 243

Query: 260 KAEGEVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGFDVRLPPTANPESLERRIAEEWAP 319
           K EG V SVN+  L+ G         N+ P+   A FD R+ P  + ++ E+++      
Sbjct: 244 K-EGAVTSVNLTKLEGGV------AYNVVPATMSASFDFRVAPDVDMKAFEKQLQRWCQE 303

Query: 320 VSRNMTFEFKQKESVLDKFGKPALTAIDSSNPWWNLLEEAVRNANGKLGNPEIFPASTDA 379
               +TFEF Q      KF +P +T  D S+PWW     A +  N  L  PEIFPA+TD+
Sbjct: 304 AGEGVTFEFAQ------KFTEPRMTPTDDSDPWWAAFSGACKAMNLTL-EPEIFPAATDS 363

Query: 380 RYFRNQGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVYESIIKVYAS 429
           RY R  G+PA+GFSPM  TP+LLHDHNE L++  +L+G+++Y  ++   AS
Sbjct: 364 RYIRAVGIPALGFSPMNRTPVLLHDHNERLHEDIFLRGVDIYTGLLSALAS 400

BLAST of Spg000942 vs. ExPASy Swiss-Prot
Match: Q6AYS7 (Aminoacylase-1A OS=Rattus norvegicus OX=10116 GN=Acy1a PE=1 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 2.7e-98
Identity = 182/409 (44.50%), Postives = 250/409 (61.12%), Query Frame = 0

Query: 22  PSSSSSIISRFQQYLQINTVQPNPQYYEAADFIISQAKSLSLESQTIEFVEGKPLILLKW 81
           P S    ++ F+QYL+I TVQPNP Y  A  F+  +A+ L L  Q IE   G  + +L W
Sbjct: 6   PESEHPSVTLFRQYLRICTVQPNPDYGSAVTFLEERARQLGLSCQKIEVAPGYVITVLTW 65

Query: 82  PGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAI 141
           PG+NP L SILLNSHTDVVP     W H P  A  DS G IYARG+QDMK V +QYLEA+
Sbjct: 66  PGTNPLLHSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGAQDMKSVSIQYLEAV 125

Query: 142 RRLKASGFQPVRSVYLSFVPDEEIGGHGGAEKFAESDEFKKLNVAIVLDEGLPSSGENYR 201
           RRLK+ G +  R+++++FVPDEE+GGH G E F +  EF+ L     LDEGL +  + + 
Sbjct: 126 RRLKSEGHRFPRTIHMTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDAFT 185

Query: 202 VFYGERSPWWLVIKAVGAPGHGAKLYDNTALENLFKSIESVRRFRASQFDLLKAG--LKA 261
           VFY ERSPWW+ + + G PGH ++  ++TA E L K + S+  FR  +   L+A   LK 
Sbjct: 186 VFYSERSPWWIRVTSTGKPGHASRFIEDTAAEKLHKVVNSILAFREKERQRLQANPHLK- 245

Query: 262 EGEVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGFDVRLPPTANPESLERRIAEEWAPVS 321
           EG V SVN+  L+ G         N+ P+   A FD R+ P  + ++ E+++        
Sbjct: 246 EGAVTSVNLTKLEGGV------AYNVVPATMSACFDFRVAPDVDMKAFEKQLQSWCQEAG 305

Query: 322 RNMTFEFKQKESVLDKFGKPALTAIDSSNPWWNLLEEAVRNANGKLGNPEIFPASTDARY 381
             +TFEF Q      KF +P +T  D ++PWW     A +  N  L  PEIFPA+TD+RY
Sbjct: 306 EGVTFEFAQ------KFTEPRMTPTDDTDPWWAAFSGACKEMNLTL-EPEIFPAATDSRY 365

Query: 382 FRNQGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVYESIIKVYAS 429
            R  G+PA+GFSPM  TP+LLHDHNE L++A +L+G+++Y  ++   AS
Sbjct: 366 IRAVGIPALGFSPMNRTPVLLHDHNERLHEAVFLRGVDIYTRLVAALAS 400

BLAST of Spg000942 vs. ExPASy Swiss-Prot
Match: Q6PTT0 (Aminoacylase-1B OS=Rattus norvegicus OX=10116 GN=Acy1b PE=1 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 8.5e-97
Identity = 179/412 (43.45%), Postives = 247/412 (59.95%), Query Frame = 0

Query: 19  EEDPSSSSSIISRFQQYLQINTVQPNPQYYEAADFIISQAKSLSLESQTIEFVEGKPLIL 78
           EE PS     ++ F+QYL+I TVQP P Y  A  F    A+ L L  Q +E   G  + +
Sbjct: 8   EEHPS-----VTLFRQYLRIRTVQPKPDYGAAVAFFEETARQLGLGCQKVEVAPGYVVTV 67

Query: 79  LKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYL 138
           L WPG+NP L SILLNSHTDVVP     W+H P  A  DS G IY RG+QDMKCV +QYL
Sbjct: 68  LTWPGTNPTLSSILLNSHTDVVPVFKEHWSHDPFEAFKDSEGYIYTRGAQDMKCVSIQYL 127

Query: 139 EAIRRLKASGFQPVRSVYLSFVPDEEIGGHGGAEKFAESDEFKKLNVAIVLDEGLPSSGE 198
           EA++RLK  G +  R+++++FVPDEE+GGH G E F +  EF  L     LDEGL +  +
Sbjct: 128 EAVKRLKVEGHRFPRTIHMTFVPDEEVGGHQGMELFVQRHEFHALRAGFALDEGLANPTD 187

Query: 199 NYRVFYGERSPWWLVIKAVGAPGHGAKLYDNTALENLFKSIESVRRFRASQFDLLKAG-- 258
            + VFY ERSPWW+ + + G PGH ++  ++TA E L K + S+  FR  ++  L++   
Sbjct: 188 AFTVFYSERSPWWVRVTSTGRPGHASRFMEDTAAEKLHKVVNSILAFREKEWQRLQSNPH 247

Query: 259 LKAEGEVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGFDVRLPPTANPESLERRIAEEWA 318
           LK EG V SVN+  L+ G         N+ P+   A FD R+ P  + ++ E ++     
Sbjct: 248 LK-EGSVTSVNLTKLEGGV------AYNVVPATMSACFDFRVAPDVDMKAFEEQLQSWCQ 307

Query: 319 PVSRNMTFEFKQKESVLDKFGKPALTAIDSSNPWWNLLEEAVRNANGKLGNPEIFPASTD 378
                +TFEF Q      KF +P +T  D ++PWW     A +     L  PEIFPA+TD
Sbjct: 308 EAGEGVTFEFAQ------KFTEPRMTPTDDTDPWWAAFSGACKEMTLTL-EPEIFPAATD 367

Query: 379 ARYFRNQGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVYESIIKVYAS 429
           +RY R  G+PA+GFSPM  TP+LLHDHNE L++A +L+G+++Y  ++   AS
Sbjct: 368 SRYIRAVGIPALGFSPMNRTPVLLHDHNERLHEAVFLRGVDIYTRLVAALAS 400

BLAST of Spg000942 vs. ExPASy Swiss-Prot
Match: Q55DP8 (Aminoacylase-1 OS=Dictyostelium discoideum OX=44689 GN=acy1 PE=2 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 7.2e-96
Identity = 178/402 (44.28%), Postives = 255/402 (63.43%), Query Frame = 0

Query: 29  ISRFQQYLQINTVQPNPQYYEAADFIISQAKSLSLESQTIEFVEGKPLILLKWPGSNPDL 88
           ++ F+++L+I T  P P Y  +  F++ +AK  ++  +      G P++L+K  G  P+L
Sbjct: 10  VTVFREFLKIRTDHPTPDYESSTKFLVEKAKEYNIPYEVYR-ETGTPIVLMKIEGLEPNL 69

Query: 89  PSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASG 148
            ++LLNSH DVVPA H  W   P  A  D  GNI+ RG+QDMKCV MQ+LE  RR+  SG
Sbjct: 70  KTVLLNSHVDVVPAVHDSWKVDPFSAWKDESGNIFGRGTQDMKCVCMQFLEVARRIVQSG 129

Query: 149 FQPVRSVYLSFVPDEEIGGHG-GAEKFAESDEFKKLNVAIVLDEGLPSSGENYRVFYGER 208
            +  R+++LSFVPDEEIGG G G EKF  +++F++LN+ + LDEGL S   ++ VFYGER
Sbjct: 130 QKLKRTLHLSFVPDEEIGGSGKGMEKFVYTEKFRQLNIGLCLDEGLASPTNDFTVFYGER 189

Query: 209 SPWWLVIKAVGAPGHGAKLYDNTALENLFKSIESVRRFRASQFDLLKAGL----KAEGEV 268
           +PWW+ I AVG  GHG++  + TA+E L ++I  +  FR  QF+ L  G     K  G+V
Sbjct: 190 APWWVHITAVGNAGHGSRFIEGTAIEKLMRTINKMLAFRQEQFESLHHGQHECGKKLGDV 249

Query: 269 VSVNMVFLKSGTP--SPTGFVMNLQPSEAEAGFDVRLPPTANPESLERRIAEEWAPVSRN 328
            S+N+  LK+G P      F  N+ P++AEAGFD+R+PPT N +    +I +EW      
Sbjct: 250 TSLNLTVLKAGIPIDHSNNFSYNVIPTQAEAGFDIRIPPTVNLDQFLDQI-KEWT-AEEG 309

Query: 329 MTFEFKQKESVLDKFGKPALTAIDSSNPWWNLLEEAVRNANGKLGNPEIFPASTDARYFR 388
           ++F+F           K  +T +DS N WW   +E+ +  +  L   EIFPA+TD+R+ R
Sbjct: 310 LSFKF------ASYIPKNEMTKLDSDNKWWENFKESCKKMDINLVT-EIFPAATDSRFIR 369

Query: 389 NQGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVYESII 424
           N G+PA GFSP+ NTPILLHDHNEFLN+  YL+GI+++  II
Sbjct: 370 NLGIPAFGFSPINNTPILLHDHNEFLNEKVYLRGIDIFMGII 401

BLAST of Spg000942 vs. ExPASy Swiss-Prot
Match: Q03154 (Aminoacylase-1 OS=Homo sapiens OX=9606 GN=ACY1 PE=1 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 2.7e-95
Identity = 178/412 (43.20%), Postives = 245/412 (59.47%), Query Frame = 0

Query: 19  EEDPSSSSSIISRFQQYLQINTVQPNPQYYEAADFIISQAKSLSLESQTIEFVEGKPLIL 78
           EE PS     ++ F+QYL+I TVQP P Y  A  F    A+ L L  Q +E   G  + +
Sbjct: 8   EEHPS-----VTLFRQYLRIRTVQPKPDYGAAVAFFEETARQLGLGCQKVEVAPGYVVTV 67

Query: 79  LKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNIYARGSQDMKCVGMQYL 138
           L WPG+NP L SILLNSHTDVVP     W+H P  A  DS G IYARG+QDMKCV +QYL
Sbjct: 68  LTWPGTNPTLSSILLNSHTDVVPVFKEHWSHDPFEAFKDSEGYIYARGAQDMKCVSIQYL 127

Query: 139 EAIRRLKASGFQPVRSVYLSFVPDEEIGGHGGAEKFAESDEFKKLNVAIVLDEGLPSSGE 198
           EA+RRLK  G +  R+++++FVPDEE+GGH G E F +  EF  L     LDEG+ +  +
Sbjct: 128 EAVRRLKVEGHRFPRTIHMTFVPDEEVGGHQGMELFVQRPEFHALRAGFALDEGIANPTD 187

Query: 199 NYRVFYGERSPWWLVIKAVGAPGHGAKLYDNTALENLFKSIESVRRFRASQFDLLKAG-- 258
            + VFY ERSPWW+ + + G PGH ++  ++TA E L K + S+  FR  ++  L++   
Sbjct: 188 AFTVFYSERSPWWVRVTSTGRPGHASRFMEDTAAEKLHKVVNSILAFREKEWQRLQSNPH 247

Query: 259 LKAEGEVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGFDVRLPPTANPESLERRIAEEWA 318
           LK EG V SVN+  L+ G         N+ P+   A FD R+ P  + ++ E ++     
Sbjct: 248 LK-EGSVTSVNLTKLEGGV------AYNVIPATMSASFDFRVAPDVDFKAFEEQLQSWCQ 307

Query: 319 PVSRNMTFEFKQKESVLDKFGKPALTAIDSSNPWWNLLEEAVRNANGKLGNPEIFPASTD 378
                +T EF Q      K+  P +T  D SNPWW       ++ N  L  PEI PA+TD
Sbjct: 308 AAGEGVTLEFAQ------KWMHPQVTPTDDSNPWWAAFSRVCKDMNLTL-EPEIMPAATD 367

Query: 379 ARYFRNQGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVYESIIKVYAS 429
            RY R  G+PA+GFSPM  TP+LLHDH+E L++A +L+G+++Y  ++   AS
Sbjct: 368 NRYIRAVGVPALGFSPMNRTPVLLHDHDERLHEAVFLRGVDIYTRLLPALAS 400

BLAST of Spg000942 vs. ExPASy TrEMBL
Match: A0A5A7TXA6 (N-acyl-L-amino-acid amidohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold133G00610 PE=3 SV=1)

HSP 1 Score: 839.7 bits (2168), Expect = 5.5e-240
Identity = 410/448 (91.52%), Postives = 424/448 (94.64%), Query Frame = 0

Query: 1   MNFK------SIITLFLFLSISTAEEDPSSSSSIISRFQQYLQINTVQPNPQYYEAADFI 60
           MNFK      SIITLF FLSIST+E+DPSS S+I+SRFQQYLQINTVQP+PQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFI 60

Query: 61  ISQAKSLSLESQTIEFVEGKPLILLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGA 120
           ISQAKSLSLES TIEFVEGKPLI+LKWPGSN DLPSILLNSHTDVVPAEHHKWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPVRSVYLSFVPDEEIGGHGGAEKF 180
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQP+RSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 181 AESDEFKKLNVAIVLDEGLPSSGENYRVFYGERSPWWLVIKAVGAPGHGAKLYDNTALEN 240
           AESDEFKKLNVAIVLDEGLPS GENYRVFYGE+SPWWLVIKAVGAPGHGA+LYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 241 LFKSIESVRRFRASQFDLLKAGLKAEGEVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF 300
           LFKSIE+VRRFRASQFDL+KAGLK EG+VVSVNMVFLKSG PSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 301 DVRLPPTANPESLERRIAEEWAPVSRNMTFEFKQKESVLDKFGKPALTAIDSSNPWWNLL 360
           DVRLPPT NPESLERRIAEEWAP SRNMTFEFKQKES+ DKFGKPALTAID SNPWWNLL
Sbjct: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360

Query: 361 EEAVRNANGKLGNPEIFPASTDARYFRNQGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 420
           EEAVRNANGKL NPEIFPASTDARYFRN GLPAIGFSPM NTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 421 GIEVYESIIKVYASYDGQKPTKSFKDEL 443
           GIEVYESIIK YASYDG K  +SFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 448

BLAST of Spg000942 vs. ExPASy TrEMBL
Match: A0A5D3BSI0 (N-acyl-L-amino-acid amidohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold264G00590 PE=3 SV=1)

HSP 1 Score: 838.2 bits (2164), Expect = 1.6e-239
Identity = 410/448 (91.52%), Postives = 424/448 (94.64%), Query Frame = 0

Query: 1   MNFK------SIITLFLFLSISTAEEDPSSSSSIISRFQQYLQINTVQPNPQYYEAADFI 60
           MNFK      SIITLF FLSIST+E++PSS S+IISRFQQYLQINTVQP+PQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQNPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 61  ISQAKSLSLESQTIEFVEGKPLILLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGA 120
           ISQAKSLSLES TIEFVEGKPLI+LKWPGSN DLPSILLNSHTDVVPAEHHKWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPVRSVYLSFVPDEEIGGHGGAEKF 180
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQP+RSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 181 AESDEFKKLNVAIVLDEGLPSSGENYRVFYGERSPWWLVIKAVGAPGHGAKLYDNTALEN 240
           AESDEFKKLNVAIVLDEGLPS GENYRVFYGE+SPWWLVIKAVGAPGHGA+LYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 241 LFKSIESVRRFRASQFDLLKAGLKAEGEVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF 300
           LFKSIE+VRRFRASQFDL+KAGLK EG+VVSVNMVFLKSG PSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 301 DVRLPPTANPESLERRIAEEWAPVSRNMTFEFKQKESVLDKFGKPALTAIDSSNPWWNLL 360
           DVRLPPT NPESLERRIAEEWAP SRNMTFEFKQKES+ DKFGKPALTAID SNPWWNLL
Sbjct: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360

Query: 361 EEAVRNANGKLGNPEIFPASTDARYFRNQGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 420
           EEAVRNANGKL NPEIFPASTDARYFRN GLPAIGFSPM NTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 421 GIEVYESIIKVYASYDGQKPTKSFKDEL 443
           GIEVYESIIK YASYDG K  +SFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 448

BLAST of Spg000942 vs. ExPASy TrEMBL
Match: A0A1S3BJ13 (N-acyl-L-amino-acid amidohydrolase OS=Cucumis melo OX=3656 GN=LOC103490443 PE=3 SV=1)

HSP 1 Score: 838.2 bits (2164), Expect = 1.6e-239
Identity = 410/448 (91.52%), Postives = 424/448 (94.64%), Query Frame = 0

Query: 1   MNFK------SIITLFLFLSISTAEEDPSSSSSIISRFQQYLQINTVQPNPQYYEAADFI 60
           MNFK      SIITLF FLSIST+E++PSS S+IISRFQQYLQINTVQP+PQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQNPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 61  ISQAKSLSLESQTIEFVEGKPLILLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGA 120
           ISQAKSLSLES TIEFVEGKPLI+LKWPGSN DLPSILLNSHTDVVPAEHHKWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPVRSVYLSFVPDEEIGGHGGAEKF 180
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQP+RSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 181 AESDEFKKLNVAIVLDEGLPSSGENYRVFYGERSPWWLVIKAVGAPGHGAKLYDNTALEN 240
           AESDEFKKLNVAIVLDEGLPS GENYRVFYGE+SPWWLVIKAVGAPGHGA+LYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 241 LFKSIESVRRFRASQFDLLKAGLKAEGEVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF 300
           LFKSIE+VRRFRASQFDL+KAGLK EG+VVSVNMVFLKSG PSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 301 DVRLPPTANPESLERRIAEEWAPVSRNMTFEFKQKESVLDKFGKPALTAIDSSNPWWNLL 360
           DVRLPPT NPESLERRIAEEWAP SRNMTFEFKQKES+ DKFGKPALTAID SNPWWNLL
Sbjct: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360

Query: 361 EEAVRNANGKLGNPEIFPASTDARYFRNQGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 420
           EEAVRNANGKL NPEIFPASTDARYFRN GLPAIGFSPM NTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 421 GIEVYESIIKVYASYDGQKPTKSFKDEL 443
           GIEVYESIIK YASYDG K  +SFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKRMESFKDEL 448

BLAST of Spg000942 vs. ExPASy TrEMBL
Match: A0A0A0KAV8 (N-acyl-L-amino-acid amidohydrolase OS=Cucumis sativus OX=3659 GN=Csa_6G117740 PE=3 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 3.9e-238
Identity = 407/448 (90.85%), Postives = 424/448 (94.64%), Query Frame = 0

Query: 1   MNFK------SIITLFLFLSISTAEEDPSSSSSIISRFQQYLQINTVQPNPQYYEAADFI 60
           MNFK      SIITLF FLSIST+E+DPSS S+IISRFQQYLQINTVQP+PQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 61  ISQAKSLSLESQTIEFVEGKPLILLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGA 120
           ISQAKSLSLES TIEFVEGKPL++LKWPGSNP+LPSILLNSHTDVVPAEH KWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLVILKWPGSNPELPSILLNSHTDVVPAEHKKWTHPPLGA 120

Query: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPVRSVYLSFVPDEEIGGHGGAEKF 180
           HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQP+RSVYLSFVPDEEIGGHGGAEKF
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 181 AESDEFKKLNVAIVLDEGLPSSGENYRVFYGERSPWWLVIKAVGAPGHGAKLYDNTALEN 240
           AESDEFKKLNVAIVLDEGLPS GENYRVFYGE+SPWWLVIKAVGAPGHGA+LYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 241 LFKSIESVRRFRASQFDLLKAGLKAEGEVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF 300
           LFKSIE+VRRFRASQFDL+KAGLK EG+VVSVNMVFLKSG PSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 301 DVRLPPTANPESLERRIAEEWAPVSRNMTFEFKQKESVLDKFGKPALTAIDSSNPWWNLL 360
           DVR+PPT NPESLERRIAEEWAP SRNMTFEFKQKES+ +KFGKPALTAID SNP WNLL
Sbjct: 301 DVRVPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPRWNLL 360

Query: 361 EEAVRNANGKLGNPEIFPASTDARYFRNQGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 420
           EEAVRNANGKL NPEIFPASTDARYFRN GLPAIGFSPM NTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 421 GIEVYESIIKVYASYDGQKPTKSFKDEL 443
           GIEVYESIIK YASYDG KP +SFKDEL
Sbjct: 421 GIEVYESIIKAYASYDGDKPMESFKDEL 448

BLAST of Spg000942 vs. ExPASy TrEMBL
Match: A0A6J1G695 (N-acyl-L-amino-acid amidohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111451263 PE=3 SV=1)

HSP 1 Score: 828.9 bits (2140), Expect = 9.7e-237
Identity = 410/449 (91.31%), Postives = 422/449 (93.99%), Query Frame = 0

Query: 1   MNFK-------SIITLFLFLSISTAEEDPSSSSSIISRFQQYLQINTVQPNPQYYEAADF 60
           MNFK       SI TLFLFLSISTAEEDP SSSSI+SRFQ+YLQINTVQPNPQYYEAADF
Sbjct: 1   MNFKSLNLLLLSITTLFLFLSISTAEEDPLSSSSIVSRFQRYLQINTVQPNPQYYEAADF 60

Query: 61  IISQAKSLSLESQTIEFVEGKPLILLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLG 120
           +ISQAKSLSLE  T+EFV GKPL+LLKWPGSNP+LPSILLNSHTDVVP EHHKWTHPPLG
Sbjct: 61  LISQAKSLSLEYHTVEFVVGKPLVLLKWPGSNPNLPSILLNSHTDVVPFEHHKWTHPPLG 120

Query: 121 AHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPVRSVYLSFVPDEEIGGHGGAEK 180
           AHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGF+PVRSVYLSFVPDEEIGGHGGAEK
Sbjct: 121 AHIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFEPVRSVYLSFVPDEEIGGHGGAEK 180

Query: 181 FAESDEFKKLNVAIVLDEGLPSSGENYRVFYGERSPWWLVIKAVGAPGHGAKLYDNTALE 240
           FAESDEFKKLNVAIVLDEGLPS GENYR FYGERSPWWLVIKAVGAPGHGAKLYDN ALE
Sbjct: 181 FAESDEFKKLNVAIVLDEGLPSPGENYRAFYGERSPWWLVIKAVGAPGHGAKLYDNAALE 240

Query: 241 NLFKSIESVRRFRASQFDLLKAGLKAEGEVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAG 300
           NLFKSIESVRRFRASQFDLLKAGLKAEGEVVSVNMV LK+GTPSPTGFVMNLQPSEAEAG
Sbjct: 241 NLFKSIESVRRFRASQFDLLKAGLKAEGEVVSVNMVSLKAGTPSPTGFVMNLQPSEAEAG 300

Query: 301 FDVRLPPTANPESLERRIAEEWAPVSRNMTFEFKQKESVLDKFGKPALTAIDSSNPWWNL 360
           FDVRLPPTA+PESLERRI EEWAPVSRNMTFEFKQKES+ DKFGKPALTAIDSSNPWWNL
Sbjct: 301 FDVRLPPTADPESLERRITEEWAPVSRNMTFEFKQKESIYDKFGKPALTAIDSSNPWWNL 360

Query: 361 LEEAVRNANGKLGNPEIFPASTDARYFRNQGLPAIGFSPMANTPILLHDHNEFLNQAEYL 420
           LEEAV NANGKLGNPEIFPASTDARYFRNQGLPAIGFSPMANTP LLHDHNEFLNQAEYL
Sbjct: 361 LEEAVSNANGKLGNPEIFPASTDARYFRNQGLPAIGFSPMANTPSLLHDHNEFLNQAEYL 420

Query: 421 KGIEVYESIIKVYASYDGQKPTKSFKDEL 443
           KGIEVYESIIK YASY G+    +FKDEL
Sbjct: 421 KGIEVYESIIKFYASYVGR---DNFKDEL 446

BLAST of Spg000942 vs. TAIR 10
Match: AT4G38220.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 656.0 bits (1691), Expect = 2.2e-188
Identity = 320/442 (72.40%), Postives = 369/442 (83.48%), Query Frame = 0

Query: 1   MNFKSIITLFLFLSISTAEEDPSSSSSIISRFQQYLQINTVQPNPQYYEAADFIISQAKS 60
           M+   ++ + + L +S    D     +I+SRFQ+YL+INTVQPNP+YY+A DFIISQAK 
Sbjct: 1   MSLLRLLLVVVVLHLSAVAGD----DAIVSRFQEYLRINTVQPNPEYYKAVDFIISQAKP 60

Query: 61  LSLESQTIEFVEGKPLILLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSHG 120
           LSLESQTIEFV+GKPL+LLKW GS+P LP+ LLNSHTDVVP E  KWTH PL AH+D HG
Sbjct: 61  LSLESQTIEFVKGKPLLLLKWVGSDPTLPAFLLNSHTDVVPFEDSKWTHHPLQAHMDHHG 120

Query: 121 NIYARGSQDMKCVGMQYLEAIRRLKASGFQPVRSVYLSFVPDEEIGGHGGAEKFAESDEF 180
           +IYARGSQDMKCVGMQYLEAIR+L+ASGF+P+RSVYLSFVPDEEIGGH GAEKFAES  F
Sbjct: 121 DIYARGSQDMKCVGMQYLEAIRKLQASGFKPLRSVYLSFVPDEEIGGHDGAEKFAESQLF 180

Query: 181 KKLNVAIVLDEGLPSSGENYRVFYGERSPWWLVIKAVGAPGHGAKLYDNTALENLFKSIE 240
           K LN+AIVLDEGLPS  E+YRVFYGERSPWWLVIKA G PGHGAKLYDN+A+ENL KSIE
Sbjct: 181 KSLNIAIVLDEGLPSPTESYRVFYGERSPWWLVIKAKGPPGHGAKLYDNSAMENLLKSIE 240

Query: 241 SVRRFRASQFDLLKAGLKAEGEVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGFDVRLPP 300
           S+RRFRASQFDLLKAG  AEG+VVSVNM FLK+GTPSPTGFVMNLQPSEAEAGFD+R+PP
Sbjct: 241 SIRRFRASQFDLLKAGGIAEGDVVSVNMAFLKAGTPSPTGFVMNLQPSEAEAGFDIRVPP 300

Query: 301 TANPESLERRIAEEWAPVSRNMTFEFKQKESVLDKFGKPALTAIDSSNPWWNLLEEAVRN 360
           + + E+LERR+ EEWAP +RNM+FEFKQK +     GK  LTA D SNPWW LLE AV+ 
Sbjct: 301 SVDAEALERRLVEEWAPAARNMSFEFKQKLT-----GKQFLTAADDSNPWWGLLENAVKE 360

Query: 361 ANGKLGNPEIFPASTDARYFRNQGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVYE 420
           A G+   PEIFPASTDARYFR  G+PA GFSP++NTP LLHDHNE+L +AEYLKGIEVY 
Sbjct: 361 AGGRTSKPEIFPASTDARYFRKAGVPAFGFSPISNTPSLLHDHNEYLGKAEYLKGIEVYV 420

Query: 421 SIIKVYASYDGQKPTKSFKDEL 443
           SIIK YASY+ +  +   +DEL
Sbjct: 421 SIIKAYASYESKSGS---RDEL 430

BLAST of Spg000942 vs. TAIR 10
Match: AT4G38220.2 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 651.0 bits (1678), Expect = 7.0e-187
Identity = 318/442 (71.95%), Postives = 367/442 (83.03%), Query Frame = 0

Query: 1   MNFKSIITLFLFLSISTAEEDPSSSSSIISRFQQYLQINTVQPNPQYYEAADFIISQAKS 60
           M+   ++ + + L +S    D     +I+SRFQ+YL+INTVQPNP+YY+A DFIISQAK 
Sbjct: 1   MSLLRLLLVVVVLHLSAVAGD----DAIVSRFQEYLRINTVQPNPEYYKAVDFIISQAKP 60

Query: 61  LSLESQTIEFVEGKPLILLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSHG 120
           LSLESQTIEFV+GKPL+LLKW GS+P LP+ LLNSHTDVVP E  KWTH PL AH+D HG
Sbjct: 61  LSLESQTIEFVKGKPLLLLKWVGSDPTLPAFLLNSHTDVVPFEDSKWTHHPLQAHMDHHG 120

Query: 121 NIYARGSQDMKCVGMQYLEAIRRLKASGFQPVRSVYLSFVPDEEIGGHGGAEKFAESDEF 180
           +IYARGSQDMKCVGMQYLEAIR+L+ASGF+P+RSVYLSFVPDEEIGGH GAEKFAES  F
Sbjct: 121 DIYARGSQDMKCVGMQYLEAIRKLQASGFKPLRSVYLSFVPDEEIGGHDGAEKFAESQLF 180

Query: 181 KKLNVAIVLDEGLPSSGENYRVFYGERSPWWLVIKAVGAPGHGAKLYDNTALENLFKSIE 240
           K LN+AIVLDEGLPS  E+YRVFYGERSPWWLVIKA G PGHGAKLYDN+A+ENL KSIE
Sbjct: 181 KSLNIAIVLDEGLPSPTESYRVFYGERSPWWLVIKAKGPPGHGAKLYDNSAMENLLKSIE 240

Query: 241 SVRRFRASQFDLLKAGLKAEGEVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGFDVRLPP 300
           S+RRFRASQFDLLKAG  AEG+VVSVNM FLK+GTPSPTGFVMNLQPSEAEAGFD+R+PP
Sbjct: 241 SIRRFRASQFDLLKAGGIAEGDVVSVNMAFLKAGTPSPTGFVMNLQPSEAEAGFDIRVPP 300

Query: 301 TANPESLERRIAEEWAPVSRNMTFEFKQKESVLDKFGKPALTAIDSSNPWWNLLEEAVRN 360
           + + E+LERR+ EEWAP +RNM+FE  Q +  L   GK  LTA D SNPWW LLE AV+ 
Sbjct: 301 SVDAEALERRLVEEWAPAARNMSFELGQFKQKLT--GKQFLTAADDSNPWWGLLENAVKE 360

Query: 361 ANGKLGNPEIFPASTDARYFRNQGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVYE 420
           A G+   PEIFPASTDARYFR  G+PA GFSP++NTP LLHDHNE+L +AEYLKGIEVY 
Sbjct: 361 AGGRTSKPEIFPASTDARYFRKAGVPAFGFSPISNTPSLLHDHNEYLGKAEYLKGIEVYV 420

Query: 421 SIIKVYASYDGQKPTKSFKDEL 443
           SIIK YASY+ +  +   +DEL
Sbjct: 421 SIIKAYASYESKSGS---RDEL 433

BLAST of Spg000942 vs. TAIR 10
Match: AT1G44820.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 531.9 bits (1369), Expect = 4.7e-151
Identity = 239/425 (56.24%), Postives = 317/425 (74.59%), Query Frame = 0

Query: 8   TLFLFLSISTAEEDPSSSSSIISRFQQYLQINTVQPNPQYYEAADFIISQAKSLSLESQT 67
           TL +F  I + +   S   + I+RFQQYL+ NT  PNP Y     F+I+QA+S+ L ++T
Sbjct: 9   TLIIFSIIFSLQSSSSEEDTPITRFQQYLRFNTAHPNPNYTAPISFLINQAQSIGLTTKT 68

Query: 68  IEFVEGKPLILLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNIYARGS 127
           IEF+ GKP++L+ W GSNP+LPSIL NSH D VPAE  KWT+PP  AH    G+IYARG+
Sbjct: 69  IEFISGKPILLITWLGSNPNLPSILFNSHLDSVPAESEKWTYPPFSAHKTIDGHIYARGA 128

Query: 128 QDMKCVGMQYLEAIRRLKASGFQPVRSVYLSFVPDEEIGGHGGAEKFAESDEFKKLNVAI 187
           QD KC+G+QYLE+IR LK+ GF P+R++++S+VP+EEIGG  G  KFA S EFK LN+  
Sbjct: 129 QDDKCIGVQYLESIRNLKSRGFSPLRTIHISYVPEEEIGGFDGMMKFAASSEFKDLNLGF 188

Query: 188 VLDEGLPSSGENYRVFYGERSPWWLVIKAVGAPGHGAKLYDNTALENLFKSIESVRRFRA 247
            +DEG  + G+ +RVFY +R PW  VIKA G PGHGAKLYDN+A+ENL KS+E + RFR 
Sbjct: 189 AMDEGQANPGDEFRVFYADRVPWHFVIKAEGIPGHGAKLYDNSAMENLMKSVELISRFRE 248

Query: 248 SQFDLLKAGLKAEGEVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGFDVRLPPTANPESL 307
           SQFD +KAG  A  EV+SVN V+LK+GTP+ TGFVMN+QPSEAEAG+D+RLPP A+P+ +
Sbjct: 249 SQFDFVKAGKAAYSEVISVNPVYLKAGTPTTTGFVMNMQPSEAEAGYDLRLPPMADPDVM 308

Query: 308 ERRIAEEWAPVSRNMTFEFKQKESVLDKFGKPALTAIDSSNPWWNLLEEAVRNANGKLGN 367
           ++RIAEEWAP  RNMT+  ++K  + D  G+P +T ++ SNPWW++ ++AV    GKL  
Sbjct: 309 KKRIAEEWAPSIRNMTYSIQEKGKLRDHLGRPIMTPVNDSNPWWSIFKQAVEAMGGKLAK 368

Query: 368 PEIFPASTDARYFRNQGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVYESIIKVYA 427
           PEI  ++TD+R+ R  G+P  GFSPM NTPILLHDHNEFL    ++KGIEVYES+I   +
Sbjct: 369 PEILASTTDSRFIRTLGIPTFGFSPMTNTPILLHDHNEFLKDTVFMKGIEVYESVISALS 428

Query: 428 SYDGQ 433
           S++G+
Sbjct: 429 SFEGE 433

BLAST of Spg000942 vs. TAIR 10
Match: AT1G44180.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 519.2 bits (1336), Expect = 3.2e-147
Identity = 241/425 (56.71%), Postives = 311/425 (73.18%), Query Frame = 0

Query: 8   TLFLFLSIST--AEEDPSSSSSIISRFQQYLQINTVQPNPQYYEAADFIISQAKSLSLES 67
           TLFLF  I +  +        + I+RFQQYL+ NT  PNP Y     F++ QA+S+ L S
Sbjct: 9   TLFLFSFIFSLQSHSKEEEEDTPITRFQQYLRFNTAHPNPNYTAPISFLLDQARSIGLTS 68

Query: 68  QTIEFVEGKPLILLKWPGSNPDLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSHGNIYAR 127
           +TIEFV GKP++LL W GSN +L SIL NSH D VPAE  KW HPP  AH    G+IYAR
Sbjct: 69  RTIEFVPGKPVLLLTWLGSNLNLSSILFNSHLDSVPAESEKWIHPPFSAHRTIDGHIYAR 128

Query: 128 GSQDMKCVGMQYLEAIRRLKASGFQPVRSVYLSFVPDEEIGGHGGAEKFAESDEFKKLNV 187
           G+QD KC+G+QYLEAIR LK+  F P+R++++S+VP+EEIGG GG  KFA S EFK LN+
Sbjct: 129 GAQDDKCIGVQYLEAIRNLKSRSFSPLRTIHISYVPEEEIGGFGGMMKFAASSEFKDLNL 188

Query: 188 AIVLDEGLPSSGENYRVFYGERSPWWLVIKAVGAPGHGAKLYDNTALENLFKSIESVRRF 247
             V+DEG  S G+ +RVFY ER+PW L I+A G PGHGAKLYDN+A+ENL KS+E + RF
Sbjct: 189 GFVMDEGQASPGDEFRVFYAERTPWHLAIRANGMPGHGAKLYDNSAMENLMKSVELISRF 248

Query: 248 RASQFDLLKAGLKAEGEVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGFDVRLPPTANPE 307
           R SQFD +KAG  A  EV+SVN V+LK+GTPS TGFVMN+QPSEAE G+D+RLPP A+P 
Sbjct: 249 RESQFDFVKAGKAANSEVISVNPVYLKAGTPSTTGFVMNMQPSEAEVGYDLRLPPMADPV 308

Query: 308 SLERRIAEEWAPVSRNMTFEFKQKESVLDKFGKPALTAIDSSNPWWNLLEEAVRNANGKL 367
           +L++RIAEEWAP  RNMT+  KQ+  + D  G+P +T  + +NPWW++ ++AV    GKL
Sbjct: 309 ALKKRIAEEWAPSIRNMTYTLKQQGKLTDHLGRPIMTTTNDTNPWWSIFKQAVEATGGKL 368

Query: 368 GNPEIFPASTDARYFRNQGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVYESIIKV 427
             PEI  ++TD+R+ R  G+P +GFSPM NTPIL+HDHNEFL    ++KGI VYES+I  
Sbjct: 369 AKPEILISTTDSRFIRTLGIPVLGFSPMINTPILVHDHNEFLKDTVFMKGIGVYESVISA 428

Query: 428 YASYD 431
            +S++
Sbjct: 429 LSSFE 433

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038876663.13.9e-24092.92aminoacylase-1 [Benincasa hispida][more]
KAA0047810.11.1e-23991.52aminoacylase-1 [Cucumis melo var. makuwa][more]
XP_008448158.13.3e-23991.52PREDICTED: aminoacylase-1 [Cucumis melo] >TYK01049.1 aminoacylase-1 [Cucumis mel... [more]
XP_004139978.18.2e-23890.85aminoacylase-1 [Cucumis sativus] >KGN46643.1 hypothetical protein Csa_005514 [Cu... [more]
XP_023532856.14.0e-23791.76aminoacylase-1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q99JW27.5e-10145.50Aminoacylase-1 OS=Mus musculus OX=10090 GN=Acy1 PE=1 SV=1[more]
Q6AYS72.7e-9844.50Aminoacylase-1A OS=Rattus norvegicus OX=10116 GN=Acy1a PE=1 SV=1[more]
Q6PTT08.5e-9743.45Aminoacylase-1B OS=Rattus norvegicus OX=10116 GN=Acy1b PE=1 SV=1[more]
Q55DP87.2e-9644.28Aminoacylase-1 OS=Dictyostelium discoideum OX=44689 GN=acy1 PE=2 SV=1[more]
Q031542.7e-9543.20Aminoacylase-1 OS=Homo sapiens OX=9606 GN=ACY1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7TXA65.5e-24091.52N-acyl-L-amino-acid amidohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A5D3BSI01.6e-23991.52N-acyl-L-amino-acid amidohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BJ131.6e-23991.52N-acyl-L-amino-acid amidohydrolase OS=Cucumis melo OX=3656 GN=LOC103490443 PE=3 ... [more]
A0A0A0KAV83.9e-23890.85N-acyl-L-amino-acid amidohydrolase OS=Cucumis sativus OX=3659 GN=Csa_6G117740 PE... [more]
A0A6J1G6959.7e-23791.31N-acyl-L-amino-acid amidohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111451263... [more]
Match NameE-valueIdentityDescription
AT4G38220.12.2e-18872.40Peptidase M20/M25/M40 family protein [more]
AT4G38220.27.0e-18771.95Peptidase M20/M25/M40 family protein [more]
AT1G44820.14.7e-15156.24Peptidase M20/M25/M40 family protein [more]
AT1G44180.13.2e-14756.71Peptidase M20/M25/M40 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.150.900coord: 348..428
e-value: 8.4E-30
score: 104.4
NoneNo IPR availablePIRSFPIRSF036696ACY-1coord: 18..431
e-value: 5.5E-177
score: 586.2
NoneNo IPR availableGENE3D3.30.70.360coord: 211..320
e-value: 3.2E-8
score: 35.4
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 19..210
e-value: 1.8E-59
score: 202.6
NoneNo IPR availablePANTHERPTHR45892:SF4BNAA06G40880D PROTEINcoord: 6..433
NoneNo IPR availablePANTHERPTHR45892AMINOACYLASE-1coord: 6..433
NoneNo IPR availableCDDcd05646M20_AcylaseI_likecoord: 31..427
e-value: 0.0
score: 662.045
NoneNo IPR availableSUPERFAMILY53187Zn-dependent exopeptidasescoord: 26..426
IPR002933Peptidase M20PFAMPF01546Peptidase_M20coord: 92..424
e-value: 2.3E-37
score: 128.7
IPR010159N-acyl-L-amino-acid amidohydrolaseTIGRFAMTIGR01880TIGR01880coord: 24..428
e-value: 2.6E-188
score: 623.6
IPR001261ArgE/DapE/ACY1/CPG2/YscS, conserved sitePROSITEPS00758ARGE_DAPE_CPG2_1coord: 91..100
IPR001261ArgE/DapE/ACY1/CPG2/YscS, conserved sitePROSITEPS00759ARGE_DAPE_CPG2_2coord: 127..166

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg000942.1Spg000942.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006520 cellular amino acid metabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004046 aminoacylase activity
molecular_function GO:0016787 hydrolase activity