Spg000941 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg000941
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Location	scaffold8: 36871054 .. 36874202 (-)
RNA-Seq Expression	Spg000941
Synteny	Spg000941

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CATTCTTCTACGTCAACGTCGATTCCTCTCTGTTGCTCCACCACCATCTCCCATGGCGATTTCTCCGCAGATCCTCCGCCTTCCCCATCGCATAGACTTCGTTTCACCGAAGACCCTCTCCGGCGCCGGAGCTCTCCAGTTCCCGAAACCCCTTTCCCTCCGGTGCACCGCCGATTCTTCGTCTTCTTCTCCGGAACCAGTCGATCCCGATTTCGATGCGAAGCTGTTCTGGAAGAACTTTACCAGAGGTGAAAACTATAATCGGAAAGGGTTTGGGCACAAGGAGGAGACTATGGAGCTCATGAGCAGCGAGTTCACGAGTGAGTTTTACGAATATTTTGTAATGATTTTAGTGATGTTTTATTGACTGTTGTACTGATTTTTGTGTTGAATGGCTCAGGTGAGGTTGTCAATACTCTGAAAGAAAATGGGAATGTGTTCACTTGGGGGAATGTTACTGTGAAATTGGCTGGAGCTCATGGCTTTTGCTGGGGAGTTGAGAGAGCTGTGCAGATTGCTTATGAGGCCAGGAAACAGTATCCGAGTGAAGAGATTTGGATTACCAATGAGATTATCCACAACCCAACTGTTAACAAGGTTCTTATCAGTTTAAATTTTCTTGCTTTCACTGTTTCATTAGTTAGACAATGACCACAAAAGTTCTAGCACATACAAATTCTCCAGATATTCACGAGTTCGGCTTGAGCTTCAAAAATGTTGAGTTGCTGTTAACTTAACTATTTTATTTAGTGACTTTAAGAGTTACTGACATGGTTATGAAAGAATGATTCATGCTGTAGTAGCACTAGAAATATTTCTGTGGTTAAGAACTTCTCCTACTACCAAGTTTAGATGATCTTTACATACTCTTTTCTTTTTGTGTTGTTTGGTCTGTTCTGCTTTTATGGTAGCCTACTACAGGGTGTAGTGTATGAAATATCATTTTGTAACTCTGAAATATTCTTTTGGTACAGCGTTTGGAGGAGATGGGAGTGCATAACATTCCTCTTGAGGAAGGAAAGAAACGATTCGATGTCGTTAATCCTGGCGATGTTGTGATTTTGCCTGCCTTTGGTGCTGCAGTTGAAGAAATGCTTGTTTTGAAGGATAAAAATGTTCAGATTGTTGACACAACTTGCCCATGGGTTTCCAAGGTACCTCCCTCCTCAGAAGGCAGTGGCCTTCCGTGAATGTTTTTTCTTTTGGTCAATGTTCCACTTATTACCATTATTGAAGGAGAAAAGGCACAATGCAGTTATTGATGCTAAGATTACACCACCTTCTTTCATGCAGGTTTGGAACGCTGTTGACAAGCACAAAAAAGGAGAATACACTTCAGTAATTCATGGTAAGTATCGTCATGAAGAAACCATAGCAACTGCTTCTTTCGCTGGAAAGTACATCATTGTAAAAGACATGGCAGAGGTACGCCCTCGTTAGTTTTGCCCTTTCCTTGACATGAAAACTTTTGGCGCATTGAGTAAGCATTTGTTAAAGTAAAATTCTGGACCTATTTCAGGCAACATATGTCTGCGACTACATTCTTGGTGCTGGATCAATTTCAACTAAAGAAGGATTTTTGAAGGTACAACATTTTAGCTATTTACTTAACCGAACTTTCTGATCTCATATGACATCATTCATGATACGAGTCAAGTTTCTGGCTTGTTTATAGAAATTCAAGTCAGCTGTTTCGAAAGGATTCGATCCAGATAATGACCTCATAAAAGTTGGCATTGCCAATCAGACCACAATGCTCAAAGGGGAAACAGAAGAGATTGGTTAGTTTTTGGAACCACTCATGCTATGTCAAACATCTTTCTGTGACAAATTTGCATTCTGTGGATTATTGATTCTTATGATTCTGATTACAGGAAAATTGATAGAGAAAACTATGATGCGGAAATATGGAGTTGAAAATATCAATGAGCACTTCATTAGTTTCAATACGATCTGTAATGCTACCCAAGTAAGTACTTCGTTCTTCTTATACAGAAGTAGTACATATTAAAGGGGTTGTGTAAAGCCTTATGTTAGTTATAAATGAGTAAGATGTCTTTGTGAGTAAGGACAACATGCCCATTGATGTTAGGTCTTTTGGGTTGGATTCAAAAGTAAATTTCATATAGACTTGGACTTTGAAGAGACTATCGTAATGTTTTAGAGTTATGGGAGGTCTGTTGTCGTCTATACCTACAGATATATATGTGTGAAAGTAGATATAAAACAAATGAAAGGAAAAAAACTTTCTTGTAGTGATTTACAGAATTGTGTGCTTACCCGAGAGAAAGATTCTTGTGATGGTGATTGGAGGTGTTGTTTTCCTTAGGAGCGCCAAGATGCAATGTACCAGCTGGTGAAGGAAGATCTGGATCTCATGCTAGTTGTTGGAGGATGGAACTCAAGCAATACCTCTCATCTTCAAGAGATTGCTGAGGCTCGAGGAATCCCGTCGTATTGGGTCGACAGCGAGCAACGTATAGGGCCAGGAAACTCAATATCATACAAGCTCATGGTAAGTCTTAACTCAGAGTGTTGATCCTTACTCGTCATCTGTTTCTACTTTCCTACTCATGTGTGCTCGTTTGATCTCACTTTAGCACGGGGAGCTGGCCGAGAAAGAAAACTGGCTACCTGATGGCCATATCACCATTGGAGTGACCTCCGGTGCCTCTACACCAGATAAGGTAAGTTACAAGTTACTGTCCAGCCTTGGAATGTTCTGTCTGTGACTGTAAATTGGTACCCTGCCATTTTTCTTGAGATTAACTTTTGTCAAATGGGTTAAAAATTATAGGTTGTTGAAAATGTTCTTAAGAAGGTTTTTGACATCAAAGGGGAAGGAACTGCGCAGCCAGCTTGAATAGCTTGGATAATAGCAAACTACAGAGTATAATTTTACTGAACTGTTTTCTTTTTGTTTTCTTTTCCTTGTTTGGACTTGTTATATGGTATGGATTAGTAGGATAATTTGCTAGATTATGTGTAATATATAAAAGATGGTGACAGAATAAATAGACTTGAGACATTATCATATAGAATAATCTTTGCAAATACTTGTCGAAGGAATATCGTAGAAAGGGAAAGTCGATAGATGTAGACCCAAAATTTCTTAAATTAATAATCTAAGGGTATGTTTGGTTTAAAAG

mRNA sequence

ATGGCGATTTCTCCGCAGATCCTCCGCCTTCCCCATCGCATAGACTTCGTTTCACCGAAGACCCTCTCCGGCGCCGGAGCTCTCCAGTTCCCGAAACCCCTTTCCCTCCGGTGCACCGCCGATTCTTCGTCTTCTTCTCCGGAACCAGTCGATCCCGATTTCGATGCGAAGCTGTTCTGGAAGAACTTTACCAGAGGTGAAAACTATAATCGGAAAGGGTTTGGGCACAAGGAGGAGACTATGGAGCTCATGAGCAGCGAGTTCACGAGTGAGGTTGTCAATACTCTGAAAGAAAATGGGAATGTGTTCACTTGGGGGAATGTTACTGTGAAATTGGCTGGAGCTCATGGCTTTTGCTGGGGAGTTGAGAGAGCTGTGCAGATTGCTTATGAGGCCAGGAAACAGTATCCGAGTGAAGAGATTTGGATTACCAATGAGATTATCCACAACCCAACTGTTAACAAGCGTTTGGAGGAGATGGGAGTGCATAACATTCCTCTTGAGGAAGGAAAGAAACGATTCGATGTCGTTAATCCTGGCGATGTTGTGATTTTGCCTGCCTTTGGTGCTGCAGTTGAAGAAATGCTTGTTTTGAAGGATAAAAATGTTCAGATTGTTGACACAACTTGCCCATGGGTTTCCAAGGAGAAAAGGCACAATGCAGTTATTGATGCTAAGATTACACCACCTTCTTTCATGCAGGTTTGGAACGCTGTTGACAAGCACAAAAAAGGAGAATACACTTCAGTAATTCATGGTAAGTATCGTCATGAAGAAACCATAGCAACTGCTTCTTTCGCTGGAAAGTACATCATTGTAAAAGACATGGCAGAGGCAACATATGTCTGCGACTACATTCTTGGTGCTGGATCAATTTCAACTAAAGAAGGATTTTTGAAGAAATTCAAGTCAGCTGTTTCGAAAGGATTCGATCCAGATAATGACCTCATAAAAGTTGGCATTGCCAATCAGACCACAATGCTCAAAGGGGAAACAGAAGAGATTGGAAAATTGATAGAGAAAACTATGATGCGGAAATATGGAGTTGAAAATATCAATGAGCACTTCATTAGTTTCAATACGATCTGTAATGCTACCCAAGAGCGCCAAGATGCAATGTACCAGCTGGTGAAGGAAGATCTGGATCTCATGCTAGTTGTTGGAGGATGGAACTCAAGCAATACCTCTCATCTTCAAGAGATTGCTGAGGCTCGAGGAATCCCGTCGTATTGGGTCGACAGCGAGCAACGTATAGGGCCAGGAAACTCAATATCATACAAGCTCATGCACGGGGAGCTGGCCGAGAAAGAAAACTGGCTACCTGATGGCCATATCACCATTGGAGTGACCTCCGGTGCCTCTACACCAGATAAGGTTGTTGAAAATGTTCTTAAGAAGGTTTTTGACATCAAAGGGGAAGGAACTGCGCAGCCAGCTTGA

Coding sequence (CDS)

ATGGCGATTTCTCCGCAGATCCTCCGCCTTCCCCATCGCATAGACTTCGTTTCACCGAAGACCCTCTCCGGCGCCGGAGCTCTCCAGTTCCCGAAACCCCTTTCCCTCCGGTGCACCGCCGATTCTTCGTCTTCTTCTCCGGAACCAGTCGATCCCGATTTCGATGCGAAGCTGTTCTGGAAGAACTTTACCAGAGGTGAAAACTATAATCGGAAAGGGTTTGGGCACAAGGAGGAGACTATGGAGCTCATGAGCAGCGAGTTCACGAGTGAGGTTGTCAATACTCTGAAAGAAAATGGGAATGTGTTCACTTGGGGGAATGTTACTGTGAAATTGGCTGGAGCTCATGGCTTTTGCTGGGGAGTTGAGAGAGCTGTGCAGATTGCTTATGAGGCCAGGAAACAGTATCCGAGTGAAGAGATTTGGATTACCAATGAGATTATCCACAACCCAACTGTTAACAAGCGTTTGGAGGAGATGGGAGTGCATAACATTCCTCTTGAGGAAGGAAAGAAACGATTCGATGTCGTTAATCCTGGCGATGTTGTGATTTTGCCTGCCTTTGGTGCTGCAGTTGAAGAAATGCTTGTTTTGAAGGATAAAAATGTTCAGATTGTTGACACAACTTGCCCATGGGTTTCCAAGGAGAAAAGGCACAATGCAGTTATTGATGCTAAGATTACACCACCTTCTTTCATGCAGGTTTGGAACGCTGTTGACAAGCACAAAAAAGGAGAATACACTTCAGTAATTCATGGTAAGTATCGTCATGAAGAAACCATAGCAACTGCTTCTTTCGCTGGAAAGTACATCATTGTAAAAGACATGGCAGAGGCAACATATGTCTGCGACTACATTCTTGGTGCTGGATCAATTTCAACTAAAGAAGGATTTTTGAAGAAATTCAAGTCAGCTGTTTCGAAAGGATTCGATCCAGATAATGACCTCATAAAAGTTGGCATTGCCAATCAGACCACAATGCTCAAAGGGGAAACAGAAGAGATTGGAAAATTGATAGAGAAAACTATGATGCGGAAATATGGAGTTGAAAATATCAATGAGCACTTCATTAGTTTCAATACGATCTGTAATGCTACCCAAGAGCGCCAAGATGCAATGTACCAGCTGGTGAAGGAAGATCTGGATCTCATGCTAGTTGTTGGAGGATGGAACTCAAGCAATACCTCTCATCTTCAAGAGATTGCTGAGGCTCGAGGAATCCCGTCGTATTGGGTCGACAGCGAGCAACGTATAGGGCCAGGAAACTCAATATCATACAAGCTCATGCACGGGGAGCTGGCCGAGAAAGAAAACTGGCTACCTGATGGCCATATCACCATTGGAGTGACCTCCGGTGCCTCTACACCAGATAAGGTTGTTGAAAATGTTCTTAAGAAGGTTTTTGACATCAAAGGGGAAGGAACTGCGCAGCCAGCTTGA

Protein sequence

MAISPQILRLPHRIDFVSPKTLSGAGALQFPKPLSLRCTADSSSSSPEPVDPDFDAKLFWKNFTRGENYNRKGFGHKEETMELMSSEFTSEVVNTLKENGNVFTWGNVTVKLAGAHGFCWGVERAVQIAYEARKQYPSEEIWITNEIIHNPTVNKRLEEMGVHNIPLEEGKKRFDVVNPGDVVILPAFGAAVEEMLVLKDKNVQIVDTTCPWVSKEKRHNAVIDAKITPPSFMQVWNAVDKHKKGEYTSVIHGKYRHEETIATASFAGKYIIVKDMAEATYVCDYILGAGSISTKEGFLKKFKSAVSKGFDPDNDLIKVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINEHFISFNTICNATQERQDAMYQLVKEDLDLMLVVGGWNSSNTSHLQEIAEARGIPSYWVDSEQRIGPGNSISYKLMHGELAEKENWLPDGHITIGVTSGASTPDKVVENVLKKVFDIKGEGTAQPA

Homology

[Close]

BLAST of Spg000941 vs. NCBI nr
Match: XP_023512039.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 813.9 bits (2101), Expect = 7.2e-232
Identity = 405/480 (84.38%), Postives = 432/480 (90.00%), Query Frame = 0


Query: 1   MAISPQILRLPHRIDFVSPKTLSGAGALQFPKPLSLRCTADSSSSSPEPVDPDFDAKLFW 60
           MAIS QI RLP R+D VS K+ SGAGAL+F KPL +RCTADSSS     V+PDFDAKLFW
Sbjct: 1   MAISLQIPRLPRRVDSVSSKSFSGAGALRFRKPLLVRCTADSSS-----VNPDFDAKLFW 60

Query: 61  KNFTRGENYNRKGFGHKEETMELMSSEFTSEVVNTLKENGNVFTWGNVTVKLAGAHGFCW 120
           KNF+RGENYNRKGFG+KEE ++LMSSEFTSEVVNTL ENGN FTWGNVTVKLAGA+GFCW
Sbjct: 61  KNFSRGENYNRKGFGYKEEALKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCW 120

Query: 121 GVERAVQIAYEARKQYPSEEIWITNEIIHNPTVNKRLEEMGVHNIPLEEGKKRFDVVNPG 180
           GVERAVQIAYEARKQ+PSE IWITNEIIHNPTVNKRLEEMGV+NIPLEEGKKRFDVVNPG
Sbjct: 121 GVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPG 180

Query: 181 DVVILPAFGAAVEEMLVLKDKNVQIVDTTCPWVSKEKRHNAVIDAKITPPSFMQVWNAVD 240
           DVVILPAFGAAV+EMLVL DK V+IVDTTCPWVSK                   VWNAVD
Sbjct: 181 DVVILPAFGAAVDEMLVLNDKKVKIVDTTCPWVSK-------------------VWNAVD 240

Query: 241 KHKKGEYTSVIHGKYRHEETIATASFAGKYIIVKDMAEATYVCDYILGA---GSISTKEG 300
           KHKKGEYTS+IHGKYRHEET+ATASFAGKYIIVKD+ EATYVCDYILG    G ISTKE 
Sbjct: 241 KHKKGEYTSIIHGKYRHEETVATASFAGKYIIVKDIVEATYVCDYILGVESNGLISTKEA 300

Query: 301 FLKKFKSAVSKGFDPDNDLIKVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINEHFI 360
           FLKKFKSAVSKGFDPD+DL+KVGIANQTTMLKGETEEIGKLIEKTMMRKYG+ENIN+HFI
Sbjct: 301 FLKKFKSAVSKGFDPDSDLMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFI 360

Query: 361 SFNTICNATQERQDAMYQLVKEDLDLMLVVGGWNSSNTSHLQEIAEARGIPSYWVDSEQR 420
           SFNTICNATQ RQDAMYQLV+ED+DLMLVVGGWNSSNTSHLQEIAEARGIPSYW+DSEQR
Sbjct: 361 SFNTICNATQVRQDAMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQR 420

Query: 421 IGPGNSISYKLMHGELAEKENWLPDGHITIGVTSGASTPDKVVENVLKKVFDIKGEGTAQ 478
           IGPGNSISYKL HGELA+KENWLPDGH+TIGVTSGASTPDKVV NVLKKVF+IKGEGTAQ
Sbjct: 421 IGPGNSISYKLNHGELAKKENWLPDGHVTIGVTSGASTPDKVVGNVLKKVFEIKGEGTAQ 456

[Close]

BLAST of Spg000941 vs. NCBI nr
Match: KAG7010948.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 807.4 bits (2084), Expect = 6.8e-230
Identity = 403/480 (83.96%), Postives = 429/480 (89.38%), Query Frame = 0


Query: 1   MAISPQILRLPHRIDFVSPKTLSGAGALQFPKPLSLRCTADSSSSSPEPVDPDFDAKLFW 60
           MAIS QI RL  R+D VS K  SG GAL+  KPL +RCTADSSS     V+PDFDAKLFW
Sbjct: 1   MAISLQIPRLHLRVDSVSSKRFSGGGALRCRKPLLVRCTADSSS-----VNPDFDAKLFW 60

Query: 61  KNFTRGENYNRKGFGHKEETMELMSSEFTSEVVNTLKENGNVFTWGNVTVKLAGAHGFCW 120
           KNF+RGENYNRKGFGHKEET++LMSSEFTSEVVNTL ENGN FTWGNVTVKLAGA+GFCW
Sbjct: 61  KNFSRGENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCW 120

Query: 121 GVERAVQIAYEARKQYPSEEIWITNEIIHNPTVNKRLEEMGVHNIPLEEGKKRFDVVNPG 180
           GVERAVQIAYEARKQ+PSE IWITNEIIHNPTVNKRLEEMGV+NIPLEEGKKRFDVVNPG
Sbjct: 121 GVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPG 180

Query: 181 DVVILPAFGAAVEEMLVLKDKNVQIVDTTCPWVSKEKRHNAVIDAKITPPSFMQVWNAVD 240
           DVVILPAFGAAV+EMLVL DK V+IVDTTCPWVSK                   VWNAVD
Sbjct: 181 DVVILPAFGAAVDEMLVLSDKKVKIVDTTCPWVSK-------------------VWNAVD 240

Query: 241 KHKKGEYTSVIHGKYRHEETIATASFAGKYIIVKDMAEATYVCDYILGA---GSISTKEG 300
           KHKKGEYTS+IHGKYRHEET+ATASFAGKYIIVKDM EATYVCDYILG    G ISTKE 
Sbjct: 241 KHKKGEYTSIIHGKYRHEETVATASFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEA 300

Query: 301 FLKKFKSAVSKGFDPDNDLIKVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINEHFI 360
           FLKKFKSA+SKGFDPD+DL+KVGIANQTTMLKGETEEIGKLIEKTMMRKYG+ENIN+HFI
Sbjct: 301 FLKKFKSALSKGFDPDSDLMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFI 360

Query: 361 SFNTICNATQERQDAMYQLVKEDLDLMLVVGGWNSSNTSHLQEIAEARGIPSYWVDSEQR 420
           SFNTICNATQ RQDAMYQLV+ED+DLMLVVGGWNSSNTSHLQEIAEARGIPSYW+DSEQR
Sbjct: 361 SFNTICNATQVRQDAMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQR 420

Query: 421 IGPGNSISYKLMHGELAEKENWLPDGHITIGVTSGASTPDKVVENVLKKVFDIKGEGTAQ 478
           IGPGNSISYKL HGELA+KENWLPDGH+TIGVTSGASTPDKVV NVLKKVF++KGEGTAQ
Sbjct: 421 IGPGNSISYKLNHGELAKKENWLPDGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQ 456

[Close]

BLAST of Spg000941 vs. NCBI nr
Match: KAG6571137.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 807.4 bits (2084), Expect = 6.8e-230
Identity = 403/480 (83.96%), Postives = 429/480 (89.38%), Query Frame = 0


Query: 1   MAISPQILRLPHRIDFVSPKTLSGAGALQFPKPLSLRCTADSSSSSPEPVDPDFDAKLFW 60
           MAIS QI RL  R+D VS K  SG GAL+  KPL +RCTADSSS     V+PDFDAKLFW
Sbjct: 1   MAISLQIPRLHLRVDSVSSKRFSGGGALRCRKPLLVRCTADSSS-----VNPDFDAKLFW 60

Query: 61  KNFTRGENYNRKGFGHKEETMELMSSEFTSEVVNTLKENGNVFTWGNVTVKLAGAHGFCW 120
           KNF+RGENYNRKGFGHKEET++LMSSEFTSEVVNTL ENGN FTWGNVTVKLAGA+GFCW
Sbjct: 61  KNFSRGENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCW 120

Query: 121 GVERAVQIAYEARKQYPSEEIWITNEIIHNPTVNKRLEEMGVHNIPLEEGKKRFDVVNPG 180
           GVERAVQIAYEARKQ+PSE IWITNEIIHNPTVNKRLEEMGV+NIPLEEGKKRFDVVNPG
Sbjct: 121 GVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPG 180

Query: 181 DVVILPAFGAAVEEMLVLKDKNVQIVDTTCPWVSKEKRHNAVIDAKITPPSFMQVWNAVD 240
           DVVILPAFGAAV+EMLVL DK V+IVDTTCPWVSK                   VWNAVD
Sbjct: 181 DVVILPAFGAAVDEMLVLSDKKVKIVDTTCPWVSK-------------------VWNAVD 240

Query: 241 KHKKGEYTSVIHGKYRHEETIATASFAGKYIIVKDMAEATYVCDYILGA---GSISTKEG 300
           KHKKGEYTS+IHGKYRHEET+ATASFAGKYI VKDMAEATYVCDYILG    G ISTKE 
Sbjct: 241 KHKKGEYTSIIHGKYRHEETVATASFAGKYIFVKDMAEATYVCDYILGVESNGLISTKEA 300

Query: 301 FLKKFKSAVSKGFDPDNDLIKVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINEHFI 360
           FLKKFKSA+SKGFDPD+DL+KVGIANQTTMLKGETEEIGKLIEKTMMRKYG+ENIN+HFI
Sbjct: 301 FLKKFKSALSKGFDPDSDLMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFI 360

Query: 361 SFNTICNATQERQDAMYQLVKEDLDLMLVVGGWNSSNTSHLQEIAEARGIPSYWVDSEQR 420
           SFNTICNATQ RQDAMYQLV+ED+DLMLVVGGWNSSNTSHLQEIAEARGIPSYW+DSEQR
Sbjct: 361 SFNTICNATQVRQDAMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQR 420

Query: 421 IGPGNSISYKLMHGELAEKENWLPDGHITIGVTSGASTPDKVVENVLKKVFDIKGEGTAQ 478
           IGPGNSISYKL HGELA+KENWLPDGH+TIGVTSGASTPDKVV NVLKKVF++KGEGTAQ
Sbjct: 421 IGPGNSISYKLNHGELAKKENWLPDGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQ 456

[Close]

BLAST of Spg000941 vs. NCBI nr
Match: XP_038902065.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X2 [Benincasa hispida])

HSP 1 Score: 805.8 bits (2080), Expect = 2.0e-229
Identity = 404/480 (84.17%), Postives = 428/480 (89.17%), Query Frame = 0


Query: 1   MAISPQILRLPHRIDFVSPKTLSGAGALQFPKPLSLRCTADSSSSSPEPVDPDFDAKLFW 60
           MAIS QI R P RIDFVS KT SG  AL+  KPL LRCTAD SSSSPEP+DPDFDAKLFW
Sbjct: 1   MAISLQIPRFPLRIDFVSRKTFSGVRALRCRKPLFLRCTAD-SSSSPEPIDPDFDAKLFW 60

Query: 61  KNFTRGENYNRKGFGHKEETMELMSSEFTSEVVNTLKENGNVFTWGNVTVKLAGAHGFCW 120
           KNF+RGENYNRKGFGHKEET++LMSSEFTSE+VNTL ENG  FTWGNVTVKLAGA+GFCW
Sbjct: 61  KNFSRGENYNRKGFGHKEETVKLMSSEFTSEIVNTLNENGKQFTWGNVTVKLAGAYGFCW 120

Query: 121 GVERAVQIAYEARKQYPSEEIWITNEIIHNPTVNKRLEEMGVHNIPLEEGKKRFDVVNPG 180
           GVERAVQIAYEARKQ+PSE IWITNEIIHNPTVNKRLEEMGV+NIPLEEGKK+F+VVNPG
Sbjct: 121 GVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKQFNVVNPG 180

Query: 181 DVVILPAFGAAVEEMLVLKDKNVQIVDTTCPWVSKEKRHNAVIDAKITPPSFMQVWNAVD 240
           DVVILPAFGAAV+EM+VL DK V+IVDTTCPWVSK                   VWNAVD
Sbjct: 181 DVVILPAFGAAVDEMIVLNDKKVKIVDTTCPWVSK-------------------VWNAVD 240

Query: 241 KHKKGEYTSVIHGKYRHEETIATASFAGKYIIVKDMAEATYVCDYILGA---GSISTKEG 300
           KHKKGEYTS+IHGKYRHEETIATASFAGKYIIVKDMAEA  VCDYILG    G ISTK  
Sbjct: 241 KHKKGEYTSIIHGKYRHEETIATASFAGKYIIVKDMAEAMDVCDYILGVEHNGLISTKAE 300

Query: 301 FLKKFKSAVSKGFDPDNDLIKVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINEHFI 360
           FLKKFKSAVSKGFDPD DL+K+GI+NQTTMLK ETEEIGKL+EKTMMRKYGVENINEHFI
Sbjct: 301 FLKKFKSAVSKGFDPDIDLMKIGISNQTTMLKRETEEIGKLMEKTMMRKYGVENINEHFI 360

Query: 361 SFNTICNATQERQDAMYQLVKEDLDLMLVVGGWNSSNTSHLQEIAEARGIPSYWVDSEQR 420
           SFNTICNATQ RQDAMY+LV+ED+DLMLVVGGWNSSNTSHLQEIAEARGIPSYW+DSEQR
Sbjct: 361 SFNTICNATQVRQDAMYELVEEDVDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQR 420

Query: 421 IGPGNSISYKLMHGELAEKENWLPDGHITIGVTSGASTPDKVVENVLKKVFDIKGEGTAQ 478
           IGPGNSISYKL HGELA KENWLPDGHITIGVTSGASTPDKVV NVLKKVFDIKG+GTAQ
Sbjct: 421 IGPGNSISYKLTHGELANKENWLPDGHITIGVTSGASTPDKVVGNVLKKVFDIKGDGTAQ 460

[Close]

BLAST of Spg000941 vs. NCBI nr
Match: XP_022944389.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 805.1 bits (2078), Expect = 3.4e-229
Identity = 402/480 (83.75%), Postives = 428/480 (89.17%), Query Frame = 0


Query: 1   MAISPQILRLPHRIDFVSPKTLSGAGALQFPKPLSLRCTADSSSSSPEPVDPDFDAKLFW 60
           MAIS QI RL  R+D VS K  S  GAL+  KPL +RCTADSSS     V+PDFDAKLFW
Sbjct: 1   MAISLQIPRLHLRVDSVSSKRFSAGGALRCRKPLLVRCTADSSS-----VNPDFDAKLFW 60

Query: 61  KNFTRGENYNRKGFGHKEETMELMSSEFTSEVVNTLKENGNVFTWGNVTVKLAGAHGFCW 120
           KNF+RGENYNRKGFGHKEET++LMSSEFTSEVVNTL ENGN FTWGNVTVKLAGA+GFCW
Sbjct: 61  KNFSRGENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCW 120

Query: 121 GVERAVQIAYEARKQYPSEEIWITNEIIHNPTVNKRLEEMGVHNIPLEEGKKRFDVVNPG 180
           GVERAVQIAYEARKQ+PSE IWITNEIIHNPTVNKRLEEMGV+NIPLEEGKKRFDVVNPG
Sbjct: 121 GVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPG 180

Query: 181 DVVILPAFGAAVEEMLVLKDKNVQIVDTTCPWVSKEKRHNAVIDAKITPPSFMQVWNAVD 240
           DVVILPAFGAAV+EMLVL DK V+IVDTTCPWVSK                   VWNAVD
Sbjct: 181 DVVILPAFGAAVDEMLVLSDKKVKIVDTTCPWVSK-------------------VWNAVD 240

Query: 241 KHKKGEYTSVIHGKYRHEETIATASFAGKYIIVKDMAEATYVCDYILGA---GSISTKEG 300
           KHKKGEYTS+IHGKYRHEET+ATASFAGKYIIVKDM EATYVCDYILG    G ISTKE 
Sbjct: 241 KHKKGEYTSIIHGKYRHEETVATASFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEA 300

Query: 301 FLKKFKSAVSKGFDPDNDLIKVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINEHFI 360
           FLKKFKSA+SKGFDPD+DL+KVGIANQTTMLKGETEEIGKLIEKTMMRKYG+ENIN+HFI
Sbjct: 301 FLKKFKSALSKGFDPDSDLMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFI 360

Query: 361 SFNTICNATQERQDAMYQLVKEDLDLMLVVGGWNSSNTSHLQEIAEARGIPSYWVDSEQR 420
           SFNTICNATQ RQDAMYQLV+ED+DLMLVVGGWNSSNTSHLQEIAEARGIPSYW+DSEQR
Sbjct: 361 SFNTICNATQVRQDAMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQR 420

Query: 421 IGPGNSISYKLMHGELAEKENWLPDGHITIGVTSGASTPDKVVENVLKKVFDIKGEGTAQ 478
           IGPGNSISYKL HGELA+KENWLPDGH+TIGVTSGASTPDKVV NVLKKVF++KGEGTAQ
Sbjct: 421 IGPGNSISYKLNHGELAKKENWLPDGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQ 456

[Close]

BLAST of Spg000941 vs. ExPASy Swiss-Prot
Match: Q94B35 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISPH PE=1 SV=1)

HSP 1 Score: 678.7 bits (1750), Expect = 4.8e-194
Identity = 344/485 (70.93%), Postives = 397/485 (81.86%), Query Frame = 0


Query: 1   MAISPQILRLPHRID-FVSPKTLSGAGALQFPKPLSLRCTA-DSSSSSPEPV-DPDFDAK 60
           MA++ Q  RL  R D FV    LSG+G+L+  K LS+RC++ D ++ SP  V D DFDAK
Sbjct: 1   MAVALQFSRLCVRPDTFVRENHLSGSGSLRRRKALSVRCSSGDENAPSPSVVMDSDFDAK 60

Query: 61  LFWKNFTRGENYNRKGFGHKEETMELMSSEFTSEVVNTLKENGNVFTWGNVTVKLAGAHG 120
           +F KN TR +NYNRKGFGHKEET++LM+ E+TS+++ TLK NG  ++WG+VTVKLA A+G
Sbjct: 61  VFRKNLTRSDNYNRKGFGHKEETLKLMNREYTSDILETLKTNGYTYSWGDVTVKLAKAYG 120

Query: 121 FCWGVERAVQIAYEARKQYPSEEIWITNEIIHNPTVNKRLEEMGVHNIPLEEGKKRFDVV 180
           FCWGVERAVQIAYEARKQ+P E +WITNEIIHNPTVNKRLE+M V  IP+E+ KK+FDVV
Sbjct: 121 FCWGVERAVQIAYEARKQFPEERLWITNEIIHNPTVNKRLEDMDVKIIPVEDSKKQFDVV 180

Query: 181 NPGDVVILPAFGAAVEEMLVLKDKNVQIVDTTCPWVSKEKRHNAVIDAKITPPSFMQVWN 240
              DVVILPAFGA V+EM VL DK VQIVDTTCPWV+K                   VWN
Sbjct: 181 EKDDVVILPAFGAGVDEMYVLNDKKVQIVDTTCPWVTK-------------------VWN 240

Query: 241 AVDKHKKGEYTSVIHGKYRHEETIATASFAGKYIIVKDMAEATYVCDYILGA---GSIST 300
            V+KHKKGEYTSVIHGKY HEETIATASFAGKYIIVK+M EA YVCDYILG    GS ST
Sbjct: 241 TVEKHKKGEYTSVIHGKYNHEETIATASFAGKYIIVKNMKEANYVCDYILGGQYDGSSST 300

Query: 301 KEGFLKKFKSAVSKGFDPDNDLIKVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINE 360
           KE F++KFK A+SKGFDPDNDL+KVGIANQTTMLKGETEEIG+L+E TMMRKYGVEN++ 
Sbjct: 301 KEEFMEKFKYAISKGFDPDNDLVKVGIANQTTMLKGETEEIGRLLETTMMRKYGVENVSG 360

Query: 361 HFISFNTICNATQERQDAMYQLVKEDLDLMLVVGGWNSSNTSHLQEIAEARGIPSYWVDS 420
           HFISFNTIC+ATQERQDA+Y+LV+E +DLMLVVGGWNSSNTSHLQEI+EARGIPSYW+DS
Sbjct: 361 HFISFNTICDATQERQDAIYELVEEKIDLMLVVGGWNSSNTSHLQEISEARGIPSYWIDS 420

Query: 421 EQRIGPGNSISYKLMHGELAEKENWLPDGHITIGVTSGASTPDKVVENVLKKVFDIKGEG 480
           E+RIGPGN I+YKL +GEL EKEN+LP G ITIGVTSGASTPDKVVE+ L KVFDIK E 
Sbjct: 421 EKRIGPGNKIAYKLHYGELVEKENFLPKGPITIGVTSGASTPDKVVEDALVKVFDIKREE 466

[Close]

BLAST of Spg000941 vs. ExPASy Swiss-Prot
Match: Q6AVG6 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISPH PE=2 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 1.7e-191
Identity = 331/452 (73.23%), Postives = 375/452 (82.96%), Query Frame = 0


Query: 33  PLSLRC--TADSSSSSPEPVDPDFDAKLFWKNFTRGENYNRKGFGHKEETMELMSSEFTS 92
           P S+RC  +A SS S+   +D DFD K F  N TR +NYNRKGFGHK+ET+ELMS E+TS
Sbjct: 27  PSSVRCDSSAASSLSASASLDADFDKKQFRHNLTRSDNYNRKGFGHKKETLELMSQEYTS 86

Query: 93  EVVNTLKENGNVFTWGNVTVKLAGAHGFCWGVERAVQIAYEARKQYPSEEIWITNEIIHN 152
           +V+ TLKENGN  TWG VTVKLA A+GFCWGVERAVQIAYEARKQ+P + IW+TNEIIHN
Sbjct: 87  DVIKTLKENGNQHTWGPVTVKLAEAYGFCWGVERAVQIAYEARKQFPDDRIWLTNEIIHN 146

Query: 153 PTVNKRLEEMGVHNIPLEEGKKRFDVVNPGDVVILPAFGAAVEEMLVLKDKNVQIVDTTC 212
           PTVNKRLE+MGV NIP++ G K FDVV  GDVV+LPAFGAAVEEM  L +K VQIVDTTC
Sbjct: 147 PTVNKRLEDMGVQNIPVDAGIKDFDVVEQGDVVVLPAFGAAVEEMYTLNEKKVQIVDTTC 206

Query: 213 PWVSKEKRHNAVIDAKITPPSFMQVWNAVDKHKKGEYTSVIHGKYRHEETIATASFAGKY 272
           PWVSK                   VWN V+KHKKG+YTS+IHGKY HEET+ATASFAG Y
Sbjct: 207 PWVSK-------------------VWNMVEKHKKGDYTSIIHGKYSHEETVATASFAGTY 266

Query: 273 IIVKDMAEATYVCDYILGA---GSISTKEGFLKKFKSAVSKGFDPDNDLIKVGIANQTTM 332
           IIVK++AEA+YVCDYILG    GS STKE FL+KFK+AVS GFDPD DL+KVGIANQTTM
Sbjct: 267 IIVKNIAEASYVCDYILGGQLDGSSSTKEEFLEKFKNAVSPGFDPDVDLVKVGIANQTTM 326

Query: 333 LKGETEEIGKLIEKTMMRKYGVENINEHFISFNTICNATQERQDAMYQLVKEDLDLMLVV 392
           LKGETEEIGKL+EKTMMR++GVEN+N+HFI+FNTIC+ATQERQDAMYQLVKE +DL+LVV
Sbjct: 327 LKGETEEIGKLVEKTMMRRFGVENVNDHFIAFNTICDATQERQDAMYQLVKEKVDLILVV 386

Query: 393 GGWNSSNTSHLQEIAEARGIPSYWVDSEQRIGPGNSISYKLMHGELAEKENWLPDGHITI 452
           GGWNSSNTSHLQEI E  GIPSYW+DSEQRIGPGN ISYKL HGEL EKENWLP+G ITI
Sbjct: 387 GGWNSSNTSHLQEIGELSGIPSYWIDSEQRIGPGNKISYKLNHGELVEKENWLPEGPITI 446

Query: 453 GVTSGASTPDKVVENVLKKVFDIKGEGTAQPA 480
           GVTSGASTPDKVVE+ L+KVF+IK +   Q A
Sbjct: 447 GVTSGASTPDKVVEDALQKVFEIKRQEVLQAA 459

[Close]

BLAST of Spg000941 vs. ExPASy Swiss-Prot
Match: B8HWD3 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) OX=395961 GN=ispH PE=3 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 4.5e-144
Identity = 250/423 (59.10%), Postives = 316/423 (74.70%), Query Frame = 0


Query: 55  DAKLFWKNFTRGENYNRKGFGHKEETMELMSSEFTSEVVNTLKENGNVFTWGNVTVKLAG 114
           D K F ++      Y+RKGFGH+ E  E++  E+ S ++  ++ENGN F  G VT++LA 
Sbjct: 2   DTKAFKRSLHSSNQYHRKGFGHEAEVTEMLRFEYQSSLIQQIRENGNRFQRGEVTIQLAE 61

Query: 115 AHGFCWGVERAVQIAYEARKQYPSEEIWITNEIIHNPTVNKRLEEMGVHNIPL-EEGKKR 174
           A GFCWGVERAV +AYE R+ +P+E IWITNEIIHNP+VN+ L EM V  IP+ E+G+K 
Sbjct: 62  AFGFCWGVERAVALAYETRQHFPTERIWITNEIIHNPSVNQNLREMQVEFIPVDEQGQKD 121

Query: 175 FDVVNPGDVVILPAFGAAVEEMLVLKDKNVQIVDTTCPWVSKEKRHNAVIDAKITPPSFM 234
           F VV   DVVILPAFGA+V+EM +L DK  +I+DTTCPWVSK                  
Sbjct: 122 FSVVAGEDVVILPAFGASVQEMQLLNDKGCKIMDTTCPWVSK------------------ 181

Query: 235 QVWNAVDKHKKGEYTSVIHGKYRHEETIATASFAGKYIIVKDMAEATYVCDYILGAGSIS 294
            VWN+V+KHKKG YTS+IHGKY+HEETIAT+S+A  Y+IV D+ EA YVC+YIL  G   
Sbjct: 182 -VWNSVEKHKKGSYTSIIHGKYQHEETIATSSYAATYLIVLDLKEAEYVCNYILNGGD-- 241

Query: 295 TKEGFLKKFKSAVSKGFDPDNDLIKVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENIN 354
            +E FL KF+ A S  FDPD DL++VGIANQTTMLKGETE+IGKL E+T+MRKYG + IN
Sbjct: 242 -REEFLHKFRHAYSPDFDPDRDLVRVGIANQTTMLKGETEQIGKLFERTLMRKYGPDQIN 301

Query: 355 EHFISFNTICNATQERQDAMYQLVKEDLDLMLVVGGWNSSNTSHLQEIAEARGIPSYWVD 414
           +HF+SFNTIC+ATQERQDAM++LV+  LDLM+V+GG+NSSNT+HLQEI+  R IPSY +D
Sbjct: 302 QHFLSFNTICDATQERQDAMFKLVEYPLDLMVVIGGFNSSNTTHLQEISIEREIPSYHID 361

Query: 415 SEQRIGPGNSISYKLMHGELAEKENWLPDGHITIGVTSGASTPDKVVENVLKKVFDIKGE 474
           S  RIGPGN + +K +H  L  KENWLP+G I +G+TSGASTPDKVV +VL+K+F +K  
Sbjct: 362 SADRIGPGNRVEHKPLHRPLEIKENWLPEGPIVVGITSGASTPDKVVSDVLEKIFALKST 402

Query: 475 GTA 477
             A
Sbjct: 422 SPA 402

[Close]

BLAST of Spg000941 vs. ExPASy Swiss-Prot
Match: B1WTZ2 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Crocosphaera subtropica (strain ATCC 51142 / BH68) OX=43989 GN=ispH PE=3 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 2.9e-143
Identity = 247/420 (58.81%), Postives = 318/420 (75.71%), Query Frame = 0


Query: 55  DAKLFWKNFTRGENYNRKGFGHKEETMELMSSEFTSEVVNTLKENGNVFTWGNVTVKLAG 114
           D K F ++  +  NY+RKGFGH+ E M  M++E+ S ++  ++EN   +  G+VT+KLA 
Sbjct: 2   DTKAFKRSLQQSNNYHRKGFGHETEVMGTMNTEYQSSLIQKIRENNYQWQEGDVTIKLAE 61

Query: 115 AHGFCWGVERAVQIAYEARKQYPSEEIWITNEIIHNPTVNKRLEEMGVHNIPLEEGKKRF 174
           A GFCWGVERAV +AYE R+ +P E+IWITNEIIHNP+VN+RL EM V  I +  G K F
Sbjct: 62  AFGFCWGVERAVAMAYETRQHFPQEKIWITNEIIHNPSVNQRLLEMNVGFIEVINGSKDF 121

Query: 175 DVVNPGDVVILPAFGAAVEEMLVLKDKNVQIVDTTCPWVSKEKRHNAVIDAKITPPSFMQ 234
            VV  GDVVILPAFGA+V EM +L DK   IVDTTCPWVSK                   
Sbjct: 122 SVVEAGDVVILPAFGASVTEMQLLNDKGCTIVDTTCPWVSK------------------- 181

Query: 235 VWNAVDKHKKGEYTSVIHGKYRHEETIATASFAGKYIIVKDMAEATYVCDYILGAGSIST 294
           VWN+V+KHKK +YTS+IHGKY+HEET+AT+SFAG Y++V ++ EA YVCDYIL  G+   
Sbjct: 182 VWNSVEKHKKKDYTSIIHGKYKHEETVATSSFAGTYLVVLNLKEAQYVCDYILNGGN--- 241

Query: 295 KEGFLKKFKSAVSKGFDPDNDLIKVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINE 354
           K+ FL+KFK+A S+GFDPD DLI++GIANQTTMLK ETE+IGKL E TM++KYG   +NE
Sbjct: 242 KDEFLEKFKNAHSEGFDPDKDLIRLGIANQTTMLKSETEQIGKLFETTMLKKYGPTELNE 301

Query: 355 HFISFNTICNATQERQDAMYQLVKEDLDLMLVVGGWNSSNTSHLQEIAEARGIPSYWVDS 414
           HF+SFNTIC+ATQERQDAM  LV+E++D+M+V+GG+NSSNT+HLQEI+  +GI SY +DS
Sbjct: 302 HFMSFNTICDATQERQDAMLNLVEEEVDVMVVIGGFNSSNTTHLQEISIEKGIDSYHIDS 361

Query: 415 EQRIGPGNSISYKLMHGELAEKENWLPDGHITIGVTSGASTPDKVVENVLKKVFDIKGEG 474
           ++RI PGN I +K +  ++  KENWLPDG I +GVTSGASTPDKVVE V++++F+ K  G
Sbjct: 362 DKRILPGNKIEHKPLEKDIETKENWLPDGKIIVGVTSGASTPDKVVEAVIERIFEEKNAG 399

[Close]

BLAST of Spg000941 vs. ExPASy Swiss-Prot
Match: Q8DK29 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=ispH PE=3 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 1.9e-142
Identity = 248/421 (58.91%), Postives = 307/421 (72.92%), Query Frame = 0


Query: 55  DAKLFWKNFTRGENYNRKGFGHKEETMELMSSEFTSEVVNTLKENGNVFTWGNVTVKLAG 114
           D + F ++    ENY+RKGFGH EE  + +  E+ S ++  ++ NG  +  G+VT++LA 
Sbjct: 2   DTRAFKRSLHSSENYHRKGFGHGEEVNQQLQGEYQSSLIQQIRANGYRWQQGDVTIRLAE 61

Query: 115 AHGFCWGVERAVQIAYEARKQYPSEEIWITNEIIHNPTVNKRLEEMGVHNIPLEEGKKRF 174
           A GFCWGVERAV +AYE R  +P+E IWITNEIIHNP+VN+RL +M V  IP+  G K F
Sbjct: 62  AFGFCWGVERAVALAYETRTHFPTERIWITNEIIHNPSVNERLRQMAVEFIPVVNGVKDF 121

Query: 175 DVVNPGDVVILPAFGAAVEEMLVLKDKNVQIVDTTCPWVSKEKRHNAVIDAKITPPSFMQ 234
             V PGDVVILPAFGA+V+EM +L ++   IVDTTCPWVSK                   
Sbjct: 122 SGVRPGDVVILPAFGASVQEMQLLNERGCTIVDTTCPWVSK------------------- 181

Query: 235 VWNAVDKHKKGEYTSVIHGKYRHEETIATASFAGKYIIVKDMAEATYVCDYILGAGSIST 294
           VW++V+KHKK  +TS+IHGKY HEETIAT+SFAG Y+IV ++ EA YVCDYIL  G    
Sbjct: 182 VWHSVEKHKKVSFTSIIHGKYNHEETIATSSFAGTYLIVLNLEEARYVCDYILHGGD--- 241

Query: 295 KEGFLKKFKSAVSKGFDPDNDLIKVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINE 354
           +  F+ KF  A S GFDPD DL++VGIANQTTMLKGETE+IGKL E+TM++KYG + +NE
Sbjct: 242 RAAFMAKFAKACSPGFDPDRDLVRVGIANQTTMLKGETEQIGKLFERTMIQKYGPDRLNE 301

Query: 355 HFISFNTICNATQERQDAMYQLVKEDLDLMLVVGGWNSSNTSHLQEIAEARGIPSYWVDS 414
           HF+SFNTIC+ATQERQDAM  LVKE LDLM+V+GG+NSSNT+HLQEIA   GIPSY +DS
Sbjct: 302 HFMSFNTICDATQERQDAMLSLVKEPLDLMVVIGGYNSSNTTHLQEIAIEHGIPSYHIDS 361

Query: 415 EQRIGPGNSISYKLMHGELAEKENWLPDGHITIGVTSGASTPDKVVENVLKKVFDIKGEG 474
             RIGPGN I +K +H      ENWLPD  ITIG+TSGASTPDKVVE VL K+F ++   
Sbjct: 362 ADRIGPGNRIEHKPLHQNPTVAENWLPDRPITIGITSGASTPDKVVEEVLNKIFALRSVA 400

Query: 475 T 476
           T
Sbjct: 422 T 400

[Close]

BLAST of Spg000941 vs. ExPASy TrEMBL
Match: A0A6J1FWL7 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448851 PE=3 SV=1)

HSP 1 Score: 805.1 bits (2078), Expect = 1.6e-229
Identity = 402/480 (83.75%), Postives = 428/480 (89.17%), Query Frame = 0


Query: 1   MAISPQILRLPHRIDFVSPKTLSGAGALQFPKPLSLRCTADSSSSSPEPVDPDFDAKLFW 60
           MAIS QI RL  R+D VS K  S  GAL+  KPL +RCTADSSS     V+PDFDAKLFW
Sbjct: 1   MAISLQIPRLHLRVDSVSSKRFSAGGALRCRKPLLVRCTADSSS-----VNPDFDAKLFW 60

Query: 61  KNFTRGENYNRKGFGHKEETMELMSSEFTSEVVNTLKENGNVFTWGNVTVKLAGAHGFCW 120
           KNF+RGENYNRKGFGHKEET++LMSSEFTSEVVNTL ENGN FTWGNVTVKLAGA+GFCW
Sbjct: 61  KNFSRGENYNRKGFGHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCW 120

Query: 121 GVERAVQIAYEARKQYPSEEIWITNEIIHNPTVNKRLEEMGVHNIPLEEGKKRFDVVNPG 180
           GVERAVQIAYEARKQ+PSE IWITNEIIHNPTVNKRLEEMGV+NIPLEEGKKRFDVVNPG
Sbjct: 121 GVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPG 180

Query: 181 DVVILPAFGAAVEEMLVLKDKNVQIVDTTCPWVSKEKRHNAVIDAKITPPSFMQVWNAVD 240
           DVVILPAFGAAV+EMLVL DK V+IVDTTCPWVSK                   VWNAVD
Sbjct: 181 DVVILPAFGAAVDEMLVLSDKKVKIVDTTCPWVSK-------------------VWNAVD 240

Query: 241 KHKKGEYTSVIHGKYRHEETIATASFAGKYIIVKDMAEATYVCDYILGA---GSISTKEG 300
           KHKKGEYTS+IHGKYRHEET+ATASFAGKYIIVKDM EATYVCDYILG    G ISTKE 
Sbjct: 241 KHKKGEYTSIIHGKYRHEETVATASFAGKYIIVKDMVEATYVCDYILGVESNGLISTKEA 300

Query: 301 FLKKFKSAVSKGFDPDNDLIKVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINEHFI 360
           FLKKFKSA+SKGFDPD+DL+KVGIANQTTMLKGETEEIGKLIEKTMMRKYG+ENIN+HFI
Sbjct: 301 FLKKFKSALSKGFDPDSDLMKVGIANQTTMLKGETEEIGKLIEKTMMRKYGIENINDHFI 360

Query: 361 SFNTICNATQERQDAMYQLVKEDLDLMLVVGGWNSSNTSHLQEIAEARGIPSYWVDSEQR 420
           SFNTICNATQ RQDAMYQLV+ED+DLMLVVGGWNSSNTSHLQEIAEARGIPSYW+DSEQR
Sbjct: 361 SFNTICNATQVRQDAMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQR 420

Query: 421 IGPGNSISYKLMHGELAEKENWLPDGHITIGVTSGASTPDKVVENVLKKVFDIKGEGTAQ 478
           IGPGNSISYKL HGELA+KENWLPDGH+TIGVTSGASTPDKVV NVLKKVF++KGEGTAQ
Sbjct: 421 IGPGNSISYKLNHGELAKKENWLPDGHVTIGVTSGASTPDKVVGNVLKKVFEMKGEGTAQ 456

[Close]

BLAST of Spg000941 vs. ExPASy TrEMBL
Match: A0A1S3CAB0 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498435 PE=3 SV=1)

HSP 1 Score: 797.7 bits (2059), Expect = 2.6e-227
Identity = 402/481 (83.58%), Postives = 425/481 (88.36%), Query Frame = 0


Query: 1   MAISPQILRLPHRIDFVSPKTLSGAGALQFPKPLSLRCTADSSSSSPEPVDPDFDAKLFW 60
           MAIS Q  R PHRIDF  PKT SG GAL+  +PL LRCTAD SSSSP+PVDPDF+AKLF 
Sbjct: 1   MAISLQSPRFPHRIDFSLPKTFSGVGALRCRQPLLLRCTAD-SSSSPKPVDPDFNAKLFL 60

Query: 61  KNFTRGENYNRKGFGHKEETMELMSSEFTSEVVNTLKENGNVFTWGNVTVKLAGAHGFCW 120
           KNF+RGENYNRKGFGHKEET++LM+SEFTSE+V TLKENG  FTWGNVTVKLAGA+GFCW
Sbjct: 61  KNFSRGENYNRKGFGHKEETVKLMNSEFTSEIVTTLKENGFQFTWGNVTVKLAGAYGFCW 120

Query: 121 GVERAVQIAYEARKQYPSEEIWITNEIIHNPTVNKRLEEMGVHNIPLEEG-KKRFDVVNP 180
           GVERAVQ AYEARKQYPSE IWITNEIIHNPTVNKRLEEMGVHNIPLEEG KKRFD VNP
Sbjct: 121 GVERAVQFAYEARKQYPSETIWITNEIIHNPTVNKRLEEMGVHNIPLEEGNKKRFDAVNP 180

Query: 181 GDVVILPAFGAAVEEMLVLKDKNVQIVDTTCPWVSKEKRHNAVIDAKITPPSFMQVWNAV 240
           GDVVILPAFGAAV+E+LVL DK V+IVDTTCPWVSK                   VWNAV
Sbjct: 181 GDVVILPAFGAAVDEILVLNDKKVKIVDTTCPWVSK-------------------VWNAV 240

Query: 241 DKHKKGEYTSVIHGKYRHEETIATASFAGKYIIVKDMAEATYVCDYILGA---GSISTKE 300
            KHKKGEYTS+IHGKY HEETIATASFAGKYIIVKDMAEA YV DYILG    GSISTKE
Sbjct: 241 GKHKKGEYTSIIHGKYSHEETIATASFAGKYIIVKDMAEAAYVSDYILGVEHDGSISTKE 300

Query: 301 GFLKKFKSAVSKGFDPDNDLIKVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINEHF 360
            FLKKFKSAVSKGFDPD  L+K+GIANQTTMLKGETEEIGKL+EKTMMRKYGVENINEHF
Sbjct: 301 EFLKKFKSAVSKGFDPDIHLVKIGIANQTTMLKGETEEIGKLMEKTMMRKYGVENINEHF 360

Query: 361 ISFNTICNATQERQDAMYQLVKEDLDLMLVVGGWNSSNTSHLQEIAEARGIPSYWVDSEQ 420
           ISFNTICNATQ RQDAMY+LVKED+DLMLVVGGWNSSNTSHLQEIAEARG+PSYW+DSEQ
Sbjct: 361 ISFNTICNATQVRQDAMYELVKEDVDLMLVVGGWNSSNTSHLQEIAEARGVPSYWIDSEQ 420

Query: 421 RIGPGNSISYKLMHGELAEKENWLPDGHITIGVTSGASTPDKVVENVLKKVFDIKGEGTA 478
           RIGPGNSISYKL HGELA KENWLPDGHITIGVTSGASTPDKVV +VLKKVFDIKG+GTA
Sbjct: 421 RIGPGNSISYKLAHGELANKENWLPDGHITIGVTSGASTPDKVVGDVLKKVFDIKGDGTA 461

[Close]

BLAST of Spg000941 vs. ExPASy TrEMBL
Match: A0A6J1JG99 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484238 PE=3 SV=1)

HSP 1 Score: 797.3 bits (2058), Expect = 3.4e-227
Identity = 401/480 (83.54%), Postives = 424/480 (88.33%), Query Frame = 0


Query: 1   MAISPQILRLPHRIDFVSPKTLSGAGALQFPKPLSLRCTADSSSSSPEPVDPDFDAKLFW 60
           MAIS QI RLP R+D VS K  SG GAL+  KPL +RCTADSSS     V+PDFDAKLFW
Sbjct: 1   MAISLQIPRLPLRVDSVSSKRFSGGGALRCWKPLLVRCTADSSS-----VNPDFDAKLFW 60

Query: 61  KNFTRGENYNRKGFGHKEETMELMSSEFTSEVVNTLKENGNVFTWGNVTVKLAGAHGFCW 120
           KNF+RGENYNRKGF HKEET++LMSSEFTSEVVNTL ENGN FTWGNVTVKLAGA+GFCW
Sbjct: 61  KNFSRGENYNRKGFEHKEETLKLMSSEFTSEVVNTLNENGNRFTWGNVTVKLAGAYGFCW 120

Query: 121 GVERAVQIAYEARKQYPSEEIWITNEIIHNPTVNKRLEEMGVHNIPLEEGKKRFDVVNPG 180
           GVERAVQIAYEARKQ+PSE IWITNEIIHNPTVNKRLEEMGV+NIPLEEGKKRFDVVNPG
Sbjct: 121 GVERAVQIAYEARKQFPSETIWITNEIIHNPTVNKRLEEMGVYNIPLEEGKKRFDVVNPG 180

Query: 181 DVVILPAFGAAVEEMLVLKDKNVQIVDTTCPWVSKEKRHNAVIDAKITPPSFMQVWNAVD 240
           DVVILPAFGAAV+EMLVL DK V IVDTTCPWVSK                   VWNAVD
Sbjct: 181 DVVILPAFGAAVDEMLVLSDKKVTIVDTTCPWVSK-------------------VWNAVD 240

Query: 241 KHKKGEYTSVIHGKYRHEETIATASFAGKYIIVKDMAEATYVCDYILGA---GSISTKEG 300
           KHKKGEYTS+IHGKY HEET+ATASFAGKYIIVKDM EATYVCDYILG    G ISTKE 
Sbjct: 241 KHKKGEYTSIIHGKYHHEETVATASFAGKYIIVKDMVEATYVCDYILGVESNGLISTKED 300

Query: 301 FLKKFKSAVSKGFDPDNDLIKVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINEHFI 360
           FLKKFKSAVSKGFDPD+DL+KVGIANQTTMLK ET+EIGKLIEKTMMRKYG+ENI+EHFI
Sbjct: 301 FLKKFKSAVSKGFDPDSDLMKVGIANQTTMLKEETKEIGKLIEKTMMRKYGIENISEHFI 360

Query: 361 SFNTICNATQERQDAMYQLVKEDLDLMLVVGGWNSSNTSHLQEIAEARGIPSYWVDSEQR 420
           SFNTICNATQ RQDAMYQLV+ED DLMLVVGGWNSSNTSHLQEIAEARGIPSYW+DSEQR
Sbjct: 361 SFNTICNATQVRQDAMYQLVEEDTDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDSEQR 420

Query: 421 IGPGNSISYKLMHGELAEKENWLPDGHITIGVTSGASTPDKVVENVLKKVFDIKGEGTAQ 478
           IGPGNSISYKL HGEL +KENWLPDGHITIGVTSGASTPD+VV NVLKKVF+IKGEGTAQ
Sbjct: 421 IGPGNSISYKLKHGELVKKENWLPDGHITIGVTSGASTPDQVVGNVLKKVFEIKGEGTAQ 456

[Close]

BLAST of Spg000941 vs. ExPASy TrEMBL
Match: A0A6J1D5C2 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111017170 PE=3 SV=1)

HSP 1 Score: 789.6 bits (2038), Expect = 7.1e-225
Identity = 398/485 (82.06%), Postives = 429/485 (88.45%), Query Frame = 0


Query: 1   MAISPQILRLPHRIDFVSPKTLSGAGA--LQFPKPLSLRCTADSSSSSPEPVDPDFDAKL 60
           MAIS QI R+PHR  F+SP  LS AGA  L+  KP SLRCTAD S SSP P+DPDFDAKL
Sbjct: 1   MAISLQIPRVPHRAAFISPSPLSAAGALPLRLRKPFSLRCTAD-SPSSPGPLDPDFDAKL 60

Query: 61  FWKNFTRGENYNRKGFGHKEETMELMSSEFTSEVVNTLKENGNVFTWGNVTVKLAGAHGF 120
           FWKN+ RGENYNRKGFGHKEETM+LM+SEFTSEV+NTL ENGN FTWGNVTVKLA A+GF
Sbjct: 61  FWKNWARGENYNRKGFGHKEETMKLMNSEFTSEVINTLNENGNEFTWGNVTVKLARAYGF 120

Query: 121 CWGVERAVQIAYEARKQYPSEEIWITNEIIHNPTVNKRLEEMGVHNIPLEEGKKRFDVVN 180
           C+GVERAVQIA+EARKQ+PS  IWITNEIIHNPTVNKRL EMGVH IPLEE KK+FDVVN
Sbjct: 121 CYGVERAVQIAFEARKQFPSRTIWITNEIIHNPTVNKRLGEMGVHYIPLEERKKQFDVVN 180

Query: 181 PGDVVILPAFGAAVEEMLVLKDKNVQIVDTTCPWVSKEKRHNAVIDAKITPPSFMQVWNA 240
           PGDVVILPAFGAAV+EMLVLKDKNVQIVDTTCPWVSK                   VWN 
Sbjct: 181 PGDVVILPAFGAAVDEMLVLKDKNVQIVDTTCPWVSK-------------------VWNV 240

Query: 241 VDKHKKGEYTSVIHGKYRHEETIATASFAGKYIIVKDMAEATYVCDYILGA---GSISTK 300
           VDKHKK EYTS+IHGKYRHEETIATASFAGKYIIVK+MAEATYV D+ILG    GSI TK
Sbjct: 241 VDKHKKREYTSIIHGKYRHEETIATASFAGKYIIVKNMAEATYVSDFILGVERNGSILTK 300

Query: 301 EGFLKKFKSAVSKGFDPDNDLIKVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINEH 360
           E FLKKF+SAVS+GFDPD DLIKVGIANQTTMLKGETEEIGKLIE+TMMRKYGVEN+NEH
Sbjct: 301 EEFLKKFESAVSRGFDPDYDLIKVGIANQTTMLKGETEEIGKLIERTMMRKYGVENVNEH 360

Query: 361 FISFNTICNATQ-ERQDAMYQLVKEDLDLMLVVGGWNSSNTSHLQEIAEARGIPSYWVDS 420
           F+SFNTIC+ATQ +RQDAMYQLV+ED+DLMLVVGGWNSSNTSHLQEIAEARGIPSYW+DS
Sbjct: 361 FVSFNTICDATQVKRQDAMYQLVEEDMDLMLVVGGWNSSNTSHLQEIAEARGIPSYWIDS 420

Query: 421 EQRIGPGNSISYKLMHGELAEKENWLPDGHITIGVTSGASTPDKVVENVLKKVFDIKGEG 480
           EQRIGPGNSISYKL++GELAEKENWLPDGH+TIGVTSGASTPDKVVENVLKKVFD+KGEG
Sbjct: 421 EQRIGPGNSISYKLIYGELAEKENWLPDGHVTIGVTSGASTPDKVVENVLKKVFDVKGEG 465

[Close]

BLAST of Spg000941 vs. ExPASy TrEMBL
Match: A0A5D3CQA5 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G001350 PE=3 SV=1)

HSP 1 Score: 741.1 bits (1912), Expect = 2.9e-210
Identity = 381/481 (79.21%), Postives = 401/481 (83.37%), Query Frame = 0


Query: 1   MAISPQILRLPHRIDFVSPKTLSGAGALQFPKPLSLRCTADSSSSSPEPVDPDFDAKLFW 60
           MAIS Q  R PHRIDF  PKT SG GAL+  +PL LRCTAD SSSSP+PVDPDF+AKLF 
Sbjct: 1   MAISLQSPRFPHRIDFSLPKTFSGVGALRCRQPLLLRCTAD-SSSSPKPVDPDFNAKLFL 60

Query: 61  KNFTRGENYNRKGFGHKEETMELMSSEFTSEVVNTLKENGNVFTWGNVTVKLAGAHGFCW 120
           KNF+RG                        E+V TLKENG  FTWGNVTVKLAGA+GFCW
Sbjct: 61  KNFSRG------------------------EIVTTLKENGFQFTWGNVTVKLAGAYGFCW 120

Query: 121 GVERAVQIAYEARKQYPSEEIWITNEIIHNPTVNKRLEEMGVHNIPLEEG-KKRFDVVNP 180
           GVERAVQ AYEARKQYPSE IWITNEIIHNPTVNKRLEEMGVHNIPLEEG KKRFD VNP
Sbjct: 121 GVERAVQFAYEARKQYPSETIWITNEIIHNPTVNKRLEEMGVHNIPLEEGNKKRFDAVNP 180

Query: 181 GDVVILPAFGAAVEEMLVLKDKNVQIVDTTCPWVSKEKRHNAVIDAKITPPSFMQVWNAV 240
           GDVVILPAFGAAV+E+LVL DK V+IVDTTCPWVSK                   VWNAV
Sbjct: 181 GDVVILPAFGAAVDEILVLNDKKVKIVDTTCPWVSK-------------------VWNAV 240

Query: 241 DKHKKGEYTSVIHGKYRHEETIATASFAGKYIIVKDMAEATYVCDYILGA---GSISTKE 300
            KHKKGEYTS+IHGKY HEETIATASFAGKYIIVKDMAEA YV DYILG    GSISTKE
Sbjct: 241 GKHKKGEYTSIIHGKYSHEETIATASFAGKYIIVKDMAEAAYVSDYILGVEHDGSISTKE 300

Query: 301 GFLKKFKSAVSKGFDPDNDLIKVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINEHF 360
            FLKKFKSAVSKGFDPD  L+K+GIANQTTMLKGETEEIGKL+EKTMMRKYGVENINEHF
Sbjct: 301 EFLKKFKSAVSKGFDPDIHLVKIGIANQTTMLKGETEEIGKLMEKTMMRKYGVENINEHF 360

Query: 361 ISFNTICNATQERQDAMYQLVKEDLDLMLVVGGWNSSNTSHLQEIAEARGIPSYWVDSEQ 420
           ISFNTICNATQ RQDAMY+LVKED+DLMLVVGGWNSSNTSHLQEIAEARG+PSYW+DSEQ
Sbjct: 361 ISFNTICNATQVRQDAMYELVKEDVDLMLVVGGWNSSNTSHLQEIAEARGVPSYWIDSEQ 420

Query: 421 RIGPGNSISYKLMHGELAEKENWLPDGHITIGVTSGASTPDKVVENVLKKVFDIKGEGTA 478
           RIGPGNSISYKL HGELA KENWLPDGHITIGVTSGASTPDKVV +VLKKVFDIKG+GTA
Sbjct: 421 RIGPGNSISYKLAHGELANKENWLPDGHITIGVTSGASTPDKVVGDVLKKVFDIKGDGTA 437

[Close]

BLAST of Spg000941 vs. TAIR 10
Match: AT4G34350.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase )

HSP 1 Score: 678.7 bits (1750), Expect = 3.4e-195
Identity = 344/485 (70.93%), Postives = 397/485 (81.86%), Query Frame = 0


Query: 1   MAISPQILRLPHRID-FVSPKTLSGAGALQFPKPLSLRCTA-DSSSSSPEPV-DPDFDAK 60
           MA++ Q  RL  R D FV    LSG+G+L+  K LS+RC++ D ++ SP  V D DFDAK
Sbjct: 1   MAVALQFSRLCVRPDTFVRENHLSGSGSLRRRKALSVRCSSGDENAPSPSVVMDSDFDAK 60

Query: 61  LFWKNFTRGENYNRKGFGHKEETMELMSSEFTSEVVNTLKENGNVFTWGNVTVKLAGAHG 120
           +F KN TR +NYNRKGFGHKEET++LM+ E+TS+++ TLK NG  ++WG+VTVKLA A+G
Sbjct: 61  VFRKNLTRSDNYNRKGFGHKEETLKLMNREYTSDILETLKTNGYTYSWGDVTVKLAKAYG 120

Query: 121 FCWGVERAVQIAYEARKQYPSEEIWITNEIIHNPTVNKRLEEMGVHNIPLEEGKKRFDVV 180
           FCWGVERAVQIAYEARKQ+P E +WITNEIIHNPTVNKRLE+M V  IP+E+ KK+FDVV
Sbjct: 121 FCWGVERAVQIAYEARKQFPEERLWITNEIIHNPTVNKRLEDMDVKIIPVEDSKKQFDVV 180

Query: 181 NPGDVVILPAFGAAVEEMLVLKDKNVQIVDTTCPWVSKEKRHNAVIDAKITPPSFMQVWN 240
              DVVILPAFGA V+EM VL DK VQIVDTTCPWV+K                   VWN
Sbjct: 181 EKDDVVILPAFGAGVDEMYVLNDKKVQIVDTTCPWVTK-------------------VWN 240

Query: 241 AVDKHKKGEYTSVIHGKYRHEETIATASFAGKYIIVKDMAEATYVCDYILGA---GSIST 300
            V+KHKKGEYTSVIHGKY HEETIATASFAGKYIIVK+M EA YVCDYILG    GS ST
Sbjct: 241 TVEKHKKGEYTSVIHGKYNHEETIATASFAGKYIIVKNMKEANYVCDYILGGQYDGSSST 300

Query: 301 KEGFLKKFKSAVSKGFDPDNDLIKVGIANQTTMLKGETEEIGKLIEKTMMRKYGVENINE 360
           KE F++KFK A+SKGFDPDNDL+KVGIANQTTMLKGETEEIG+L+E TMMRKYGVEN++ 
Sbjct: 301 KEEFMEKFKYAISKGFDPDNDLVKVGIANQTTMLKGETEEIGRLLETTMMRKYGVENVSG 360

Query: 361 HFISFNTICNATQERQDAMYQLVKEDLDLMLVVGGWNSSNTSHLQEIAEARGIPSYWVDS 420
           HFISFNTIC+ATQERQDA+Y+LV+E +DLMLVVGGWNSSNTSHLQEI+EARGIPSYW+DS
Sbjct: 361 HFISFNTICDATQERQDAIYELVEEKIDLMLVVGGWNSSNTSHLQEISEARGIPSYWIDS 420

Query: 421 EQRIGPGNSISYKLMHGELAEKENWLPDGHITIGVTSGASTPDKVVENVLKKVFDIKGEG 480
           E+RIGPGN I+YKL +GEL EKEN+LP G ITIGVTSGASTPDKVVE+ L KVFDIK E 
Sbjct: 421 EKRIGPGNKIAYKLHYGELVEKENFLPKGPITIGVTSGASTPDKVVEDALVKVFDIKREE 466

The following BLAST results are available for this feature:

BLAST of Spg000941 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023512039.1	7.2e-232	84.38	4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X...	[more]
KAG7010948.1	6.8e-230	83.96	4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic, partial [Cucu...	[more]
KAG6571137.1	6.8e-230	83.96	4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic, partial [Cucu...	[more]
XP_038902065.1	2.0e-229	84.17	4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X...	[more]
XP_022944389.1	3.4e-229	83.75	4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X...	[more]

BLAST of Spg000941 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94B35	4.8e-194	70.93	4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Arabidopsis...	[more]
Q6AVG6	1.7e-191	73.23	4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Oryza sativ...	[more]
B8HWD3	4.5e-144	59.10	4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain PCC...	[more]
B1WTZ2	2.9e-143	58.81	4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Crocosphaera subtropica (s...	[more]
Q8DK29	1.9e-142	58.91	4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Thermosynechococcus elonga...	[more]

BLAST of Spg000941 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FWL7	1.6e-229	83.75	4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X...	[more]
A0A1S3CAB0	2.6e-227	83.58	4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X...	[more]
A0A6J1JG99	3.4e-227	83.54	4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like isoform X...	[more]
A0A6J1D5C2	7.1e-225	82.06	4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like OS=Momord...	[more]
A0A5D3CQA5	2.9e-210	79.21	4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cucumis melo var. makuwa O...	[more]

BLAST of Spg000941 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G34350.1	3.4e-195	70.93	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003451	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	PFAM	PF02401	LYTB	coord: 110..218 e-value: 2.8E-28 score: 98.9 coord: 219..467 e-value: 4.4E-51 score: 173.7
IPR003451	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	HAMAP	MF_00191	IspH	coord: 108..471 score: 27.904083
IPR003451	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	CDD	cd13944	lytB_ispH	coord: 110..467 e-value: 8.79998E-106 score: 314.348
None	No IPR available	GENE3D	3.40.1010.20		coord: 360..477 e-value: 9.6E-37 score: 127.2
None	No IPR available	GENE3D	3.40.1010.20		coord: 230..359 e-value: 8.2E-16 score: 59.9
None	No IPR available	GENE3D	3.40.50.11270		coord: 119..228 e-value: 5.3E-28 score: 99.1
None	No IPR available	PANTHER	PTHR31619	4-HYDROXY-3-METHYLBUT-2-ENYL DIPHOSPHATE REDUCTASE, CHLOROPLASTIC	coord: 10..473

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg000941.1	Spg000941.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050992	dimethylallyl diphosphate biosynthetic process
biological_process	GO:0019288	isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0051745	4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity
molecular_function	GO:0046429	4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0046872	metal ion binding