Spg000474 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg000474
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionLipoyl(octanoyl) transferase
Locationscaffold8: 43880524 .. 43886174 (+)
RNA-Seq ExpressionSpg000474
SyntenySpg000474
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAGGTCCCATTTTCATGACCATTAGCACACTCACATCAGGATTTCACTGCTCCAAAAATTTCAATTCTCTCCCTGACAAAACTTCAAATTTCTTATCTTCCTCCTAGGATTTCAGTTCCCTCACATAATCGATTTCACTGCTCTCCCAAACATTCTTCCGCACACGCTTGCAAAATCGAAAATCACACAAAATCCCAAAATTGGGAATTTGAAGAATTTGGAATCGCATCCTTCTTCTAGTTCAGACATTTGAATACTCGCAAAGCTGCAAATCGCCGTCATCCGACTTCTCATCTTGCAAGGTTAGTGTGTGAATCTACGCCTCCCTGAGTTTAACATCACAAAGGTTTTCTTTCGTGCTGGATTTCCTATTTCCTTTGCTTACAAAATCATCAAATAAACACTCCTTTCAATTCCATCTTCAAATATTTGTGCTGAATGTCGATTTTTTCTAGGGTTATTGAATCCCTTTCACTTTCACTCTCTAATCTCTTGAAATTGAAAGGAATTTTCATTTAGCATTTTGATTTGTATGTGTTGAAGGGTTGAACTAATTCCAATTTGGTTTTGACTGATGCCAATGGCACTGTCCTTTGGTCCACCGTCACAGTCTCTAACGCTGAAATCGGACTATTACTTCAAATTCAACAACGACAATGTGTTGAATCTCATATAGATGAATTTGGTGTTTGAGTATTGTTTTGTTTCTTACCTCTTTGGTGAAGAGATCATCGACATTGAAGTCGAGCCTTGGATTGACTGCAACTAACTTCATGGACAAGAGCTGTTTGTGAAAATGCCACAGAGAGAATTTTTAGATTTCAGAGTGGGTTTTTTCAATGGAAAATAGTGTTTTCGAGGCCAGAGATTAAGGAAATTTGAAATTTACCTTGACTTGTCTTTGAAGAGATTGAACATAGTTTATGATCTCATCGAGCATTCCAGCTTTTCTAGTGATTTTGTTGCATCCTGGTACTAAATCTTGCAATTACTTCATTCTCTCACGGATTTTTTCCTTTCTAGCCTACATGAAACCGAAAATAACTTATGCTTACTCTTTTACACCTATTTAGTCACTGATGTATATAATGCTATATTCTAAAGAAAATACTTGAAACAAACACACTGTTTTGGATTATATATTGAGTGTTCTTATTTTATTTTATTTTATTTTTTTTGAGAAAATATTGAATGTTCTTATTGAATATAAAGTTGTTGGCAACTAAAATATATTTATCTATTTTAATTTCACTTCAAAAAATAGTTTTTTTCAATAGATACTTGGCTATCAATGGTAGTTTAGCAATGTTTGTTGATCTGAAGCTTTTGGATGAGAAAAAAAAAATCTAATCTCTCCTAGGTAACTTATAGACCATTAAACATCAACATATCGGTGTTTAGGTTGGTAGTTCTCATTTTTCATTTGGGTTAGATTTTTCCCTTCCTAATTCTTGCAAAAGTTGGTTAATTTTTTTTTATTTTTAAAATTAATTAATTATTATCATAGTGTTTGTTGTTGGTTCACTAGGGATCTAATCTTAAATTGTATTTTTGTTTAAAGTATTTGGTAGTGGGTGGTTAAGGGTGGAGTGGTGCTTCGTGCTGGGGTGTGATTTAGAAAAAAAATCTTTGTTTATGAACATCGTCTGGTTAGCTTGGATTGTTCATACAAGTGCTTTACATATGAGAGTAGTAATATGTATGCATGAAAGCAATAGAAGAATTATACCAGAGAGCATAAGAAACTCGAGGATAAAACAAAAAAACTGAAAGAATACAGAAATGGGAGGATAAAATGTATACTCCCCCGAGAGCCAAAGGAGTAACAGATAAGCTTTCCAATTGACTTCGATTAGGAGAAAATTAACCAATAGAAAAAAGTTTGGATATGGAATTCCACTTAGGAGGTCAAAAGTTCCGTTAATTTAGCATGGTGAAAATCGTTCTTGTATCTAAACTTCCAAAATGCCATGCTTCCTCTTCAATCAAGGCATTGAAATCGTGGGCAGGGCACTTAACCATAGAACATGGCTGAACTTCGAGACAAGCCTAAGAGTGTGGATCACAAGAAATGTTGTAATTTGAGGGGAAGAAGACATCACCTAAGATGAAAATTCTTTTGTGCCGCGCTTCCATAGAAAACCACATTAAAGAACTTTGTACAAGGCTTTTCTATTTACTTTGACACATTGAAGAAGATAAGCAATAACTTAGTTTGCCTCTAGCATCAGCCTCTCAAAAGTTTACCTCTAGCATCAGTAACCAAGTCAATCAGAACCACCACAACTTTGTAGGTCACATGATGACTGATTTCTTTAAACCACATATGTATGCTTTACATGTGTCATGTTTGTGACATGATGACTGATTTTAAAGAAATGATCCAACAATTTAATGATAGTTTAAATTAAAATGAAAATAAACGAAAATTAAGGATTCATTTGAAACTTATGCCATAGTTTAAGCATTAAAAATATGACTTAGGCATTCTCCTATTCAGCTACCAAATAACTGAGCGTTTCTTATTCTAATTTTATCAATTCTACAGGATGAAAATGTATTCTTCAAAGATTATGCTGTCTCACACAAAAAACTTTCAGAGCTAGTGTTCACTCTAGGTTCTGGCAGCAATTAAGGACAACACTATTGGCACAAGGTTTTGTACATAGTCGTCCCTATGGAATCTATATTTATAATACTCCATTTACTGTTAGAAAAACCAATATGCACTTGCACTAGAAACAATTTTCTCCTTGAGGAGTTGTGCAGTCCATCAACTACACTCCCAAAATTAATACAAATTCAATTTCATTTCACGAACTAAATTTCTTTTCGAACTTGTGTGAGCAAGGTTAATTTCGTGTAAAGGACAACAAAAAGAAAGTCTTTAAAAAGGCTAGAGAAGAAAAAGAAATTTCCTTTTTCTTTTATCCTTTTTGTTACAATTATAAAATCTTAGCAAAAACCAATTTATAATGATACCTACTGTACTACAGGTTAATTTTATTGGTTTTTGTTGGTTGTATTGTACATTATTCTACTTAGGAGTATCGAGTCCTAGATGATCATTTTAGCTCTCCATTTCCTCTAGATTTTGCTTTTTATCTTATTGGGTTTGTATATATTCTTTTGTATCTTGAGTCTTTGAGATCTTTCATGTTTTCTTTTTCATCTTTTCGTTACTAATACTGTTTAAATTAAGTTTAAGCTTGCTTTGGAAAAGTAAGGTGGAGGAGGCTATTGTGCTTGCTTTGCTTAACTTGATGAGTTTGTTACAATGCTTTGACTAGATTTTTCACTTAAGTCGTTGATGTTGGGGAAATTTTTTTCTTAGAGCTATGTAGAAAGTAATTACTTATGGTAAACTTCACTCACTGGACTAGTATTCAAGTTTTCTCAACCTAGTTCAACGTAGAAATGGACCCAAATTGTTTTTGGACTGGAAATGAGTCTTCATGGAGCAAGAGATACTCCCCCCTCCCTTTTTCTTTTTGTCTCTCTTAAACTTTTTCTTTTTCCAGTGTAAAATTAAAAATGTATCATTTCTTTTATTTTATAATTAAATCCTAAATGTTAATTTGTATGAATTAAAGCTTGCCCTTCCATGAAAAAGCTTTTAGCCTCATCCCTGCTTTGGACCTCACGTAATCTTTAAGTTGACATTGCTAAGTTTTCCCGTGCTTGATTTTCTAATTTGTCTTGCTCGAGTATAGGCTTGCTCCTAGCCACCCTTCCATTTTATATTTTCTTTCCCATAAATATTATTAATTTTTTTTTTAAAATGAGAGTTCCCATAAATACTACTGTTTCTCATCTACTGAAAAACACTTCAGGCAAGTCGCAAGACTGCTGGGGGTTTTTTGTTATTTTTGTGTAATCAAACATTCTCAATATAGATCAGTGCCTCACTCTGACGGTGGCGCAACTCCCTGTTGCCATGGCGGCACAACGGCAGCGACGCGACAACTGATTTCATTCTGTCATGGCGGCGCGGCGTACCAGATCTGTAACCCCGCGGCTTGGCGACATAATGCACGGCGGACAGCAACGGTGGTGCGGGAGAGCTGCACCGGTTCCAATAGGCATCGATGCTAAATACAGAGAGTAGTATGTATATGTGTTGAGTTCATTTTTGTTGGGTTCTTGCGTTGTTTGTTTGTGTTTGTCTAAATAACGCAATTCAGGAGGGAGATGGAAATTGGAAAGCAAAGTAGTATGAATCTGTGTAGAAATTGATGAAAATGATGGCTGATTCATATTATCGTAGGGTACGATGAATTGATGAAAATGAAGGGGAAAGTCTGAATTGATTAAAAATATGAAGTTCTTTTAGTTAATTGCCCCTGCTTGGCTCCTGTTTAATTTTTTGGACATTTCCTCTGACAAATGAGATTTCCTCAAGGATTCATTACTTCTAAATAATGATTTTAAAATCTGACATAGAGTTTGTAATCACCATTGGTATATAAGTGCTAGGAATTGATCATCATCAAAACACTTGTAAGTAAATTTCAGATTTTCAAATTCAAAAGGGACAGACTGAGAAATAAAACTTAAGCCCCTTTCTAATAGACTATAGATGATAGATTTCCATATGAGTGTGAATTTCAAGAGTTCTCTCTGTTCTTTTCATTGATTTTTGCTGTGTAATTTGTCAGGTGTGAATCTACTTCAATTCTTGAAGCAATACATTTCGCTTTCCTTTCATGTCCATCTGTCGTTAAGCAAATCAAGCACTCTCTGGAATCAATCCCAACAATGAAAGCATCGTATGGAGGTACATTTCAATTTTACTTCGCAAAGAATCGAATCTGAATTCCTGAAATCTGTTGCCTGTGGTTATAATCTCTGATTCCATCAAAGAGGTGATGTTCAAGAGATAGAAAGATAGAAACAGTTAAAATCATTTTGACAACTTTAAAATAGGGAATGAGATTTCCAAGAACTCTAAAGATATGGAAAATGGGGGTTATCAATTATAGAGATGCACTCCATCTGCAAGAGAAACTTGTCTCTGCTAGAAAAGCTAGTAAAATCCCAGACACTCTCTTGTCTCTGCAACATCCACCCACATACACACTCGGAAAAAGGCGAACCGATCATAATCTGCTAATCCCCGAGTCTGATCTAGAGAAGCTAGGAGCTGAACTTCACTACACAGAGAGAGGGGGAGACATTACATTTCATGGTCCAAACCAAGCCATTTTGTACCCAATAATTTCCCTTAGAGATGTTGGAATTGGGGCCAGAAAGTATGTGGAGAAACTGGAACTGACCATGATTGAGATGGCATGCCTGTATGGAGTGAAAGCCAGAGCTGGGGAGAAGGGGGAAACTGGAGTTTGGGTTGGAGATAGAAAGATTGGAGCCATTGGAGTTCGAATCTCAAGTGGGATTACTTCTCATGGTTTGGCTTTCAATGTGGATCCTGATTTGAGGTATTTTAAGCATATTGTGCCTTGTGGGATTGCTGATAAAGATGTGACATCTTTGAGAAGGGAGACTGAGGAAGTGCTTCCTAGTGAAGCAGTTGTGGAAGAGCAGTTGATTTCTTGTTTTGTTAAGCTATTTGGTTATTGTAGAACTCAGGTGGAGGAGAATCCTTCCATTGAATTAGATTTGGAGAACAGGATTGAGAGTTTGAAATAA

mRNA sequence

ATGAAAGCATCGTATGGAGAGAAACTTGTCTCTGCTAGAAAAGCTAGTAAAATCCCAGACACTCTCTTGTCTCTGCAACATCCACCCACATACACACTCGGAAAAAGGCGAACCGATCATAATCTGCTAATCCCCGAGTCTGATCTAGAGAAGCTAGGAGCTGAACTTCACTACACAGAGAGAGGGGGAGACATTACATTTCATGGTCCAAACCAAGCCATTTTGTACCCAATAATTTCCCTTAGAGATGTTGGAATTGGGGCCAGAAAGTATGTGGAGAAACTGGAACTGACCATGATTGAGATGGCATGCCTGTATGGAGTGAAAGCCAGAGCTGGGGAGAAGGGGGAAACTGGAGTTTGGGTTGGAGATAGAAAGATTGGAGCCATTGGAGTTCGAATCTCAAGTGGGATTACTTCTCATGGTTTGGCTTTCAATGTGGATCCTGATTTGAGGTATTTTAAGCATATTGTGCCTTGTGGGATTGCTGATAAAGATGTGACATCTTTGAGAAGGGAGACTGAGGAAGTGCTTCCTAGTGAAGCAGTTGTGGAAGAGCAGTTGATTTCTTGTTTTGTTAAGCTATTTGGTTATTGTAGAACTCAGGTGGAGGAGAATCCTTCCATTGAATTAGATTTGGAGAACAGGATTGAGAGTTTGAAATAA

Coding sequence (CDS)

ATGAAAGCATCGTATGGAGAGAAACTTGTCTCTGCTAGAAAAGCTAGTAAAATCCCAGACACTCTCTTGTCTCTGCAACATCCACCCACATACACACTCGGAAAAAGGCGAACCGATCATAATCTGCTAATCCCCGAGTCTGATCTAGAGAAGCTAGGAGCTGAACTTCACTACACAGAGAGAGGGGGAGACATTACATTTCATGGTCCAAACCAAGCCATTTTGTACCCAATAATTTCCCTTAGAGATGTTGGAATTGGGGCCAGAAAGTATGTGGAGAAACTGGAACTGACCATGATTGAGATGGCATGCCTGTATGGAGTGAAAGCCAGAGCTGGGGAGAAGGGGGAAACTGGAGTTTGGGTTGGAGATAGAAAGATTGGAGCCATTGGAGTTCGAATCTCAAGTGGGATTACTTCTCATGGTTTGGCTTTCAATGTGGATCCTGATTTGAGGTATTTTAAGCATATTGTGCCTTGTGGGATTGCTGATAAAGATGTGACATCTTTGAGAAGGGAGACTGAGGAAGTGCTTCCTAGTGAAGCAGTTGTGGAAGAGCAGTTGATTTCTTGTTTTGTTAAGCTATTTGGTTATTGTAGAACTCAGGTGGAGGAGAATCCTTCCATTGAATTAGATTTGGAGAACAGGATTGAGAGTTTGAAATAA

Protein sequence

MKASYGEKLVSARKASKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLGAELHYTERGGDITFHGPNQAILYPIISLRDVGIGARKYVEKLELTMIEMACLYGVKARAGEKGETGVWVGDRKIGAIGVRISSGITSHGLAFNVDPDLRYFKHIVPCGIADKDVTSLRRETEEVLPSEAVVEEQLISCFVKLFGYCRTQVEENPSIELDLENRIESLK
Homology
BLAST of Spg000474 vs. NCBI nr
Match: XP_038879976.1 (octanoyltransferase LIP2, mitochondrial [Benincasa hispida] >XP_038879977.1 octanoyltransferase LIP2, mitochondrial [Benincasa hispida])

HSP 1 Score: 404.8 bits (1039), Expect = 4.7e-109
Identity = 196/211 (92.89%), Postives = 205/211 (97.16%), Query Frame = 0

Query: 7   EKLVSARKASKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLGAELHYTERGGDIT 66
           EKLVSARKASKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLGAELHYTERGGDIT
Sbjct: 25  EKLVSARKASKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLGAELHYTERGGDIT 84

Query: 67  FHGPNQAILYPIISLRDVGIGARKYVEKLELTMIEMACLYGVKARAGEKGETGVWVGDRK 126
           FHGPNQ+ILYPIISLRDVG GARKYVEKLELTMIEMACLYGVKARAGEKGETGVWVGDRK
Sbjct: 85  FHGPNQSILYPIISLRDVGFGARKYVEKLELTMIEMACLYGVKARAGEKGETGVWVGDRK 144

Query: 127 IGAIGVRISSGITSHGLAFNVDPDLRYFKHIVPCGIADKDVTSLRRETEEVLPSEAVVEE 186
           IGAIGVRIS+GITSHGLAFNVDPDLRYFKHIVPCGIADKDVTSLR+E+ EVLPSE V+EE
Sbjct: 145 IGAIGVRISNGITSHGLAFNVDPDLRYFKHIVPCGIADKDVTSLRKESVEVLPSEEVMEE 204

Query: 187 QLISCFVKLFGYCRTQVEENPSIELDLENRI 218
           QLISCFVKLFGYC+TQVE+NP+IELD  + I
Sbjct: 205 QLISCFVKLFGYCKTQVEKNPTIELDFGDEI 235

BLAST of Spg000474 vs. NCBI nr
Match: KAE8653496.1 (hypothetical protein Csa_007704 [Cucumis sativus])

HSP 1 Score: 391.7 bits (1005), Expect = 4.1e-105
Identity = 190/215 (88.37%), Postives = 202/215 (93.95%), Query Frame = 0

Query: 7   EKLVSARKASKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLGAELHYTERGGDIT 66
           EKLVSARK+SKIPD LLSLQHPPTYTLGKRRTDHNLLIPESDL KLGAELHY+ERGGDIT
Sbjct: 52  EKLVSARKSSKIPDMLLSLQHPPTYTLGKRRTDHNLLIPESDLAKLGAELHYSERGGDIT 111

Query: 67  FHGPNQAILYPIISLRDVGIGARKYVEKLELTMIEMACLYGVKARAGEKGETGVWVGDRK 126
           FHGPNQ+ILYPIISLRDVG GARKYVEKLE TMIEMACLYGVKA+AGEKGETGVWVGDRK
Sbjct: 112 FHGPNQSILYPIISLRDVGFGARKYVEKLEQTMIEMACLYGVKAKAGEKGETGVWVGDRK 171

Query: 127 IGAIGVRISSGITSHGLAFNVDPDLRYFKHIVPCGIADKDVTSLRRETEEVLPSEAVVEE 186
           IGAIGVRIS+GITSHGLAFNVDPDL YFKHIVPCGI DKDVTSLR+ET+EVLPSE V+EE
Sbjct: 172 IGAIGVRISNGITSHGLAFNVDPDLMYFKHIVPCGIVDKDVTSLRKETKEVLPSEEVLEE 231

Query: 187 QLISCFVKLFGYCRTQVEENPSIELDLENRIESLK 222
           QLISCFVKLFGYC+TQVE+ P  ELD  + IE+LK
Sbjct: 232 QLISCFVKLFGYCKTQVEKIPLTELDFGDEIENLK 266

BLAST of Spg000474 vs. NCBI nr
Match: TYK26906.1 (octanoyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 390.2 bits (1001), Expect = 1.2e-104
Identity = 190/215 (88.37%), Postives = 201/215 (93.49%), Query Frame = 0

Query: 7   EKLVSARKASKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLGAELHYTERGGDIT 66
           EKLVSARK+SKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDL KLGAELHYTERGGDIT
Sbjct: 25  EKLVSARKSSKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLAKLGAELHYTERGGDIT 84

Query: 67  FHGPNQAILYPIISLRDVGIGARKYVEKLELTMIEMACLYGVKARAGEKGETGVWVGDRK 126
           FHGPNQ+ILYPIISLRDVG GARKYVEKLE TMIEMACLYGVKARAGEKGETGVWVGDRK
Sbjct: 85  FHGPNQSILYPIISLRDVGFGARKYVEKLEQTMIEMACLYGVKARAGEKGETGVWVGDRK 144

Query: 127 IGAIGVRISSGITSHGLAFNVDPDLRYFKHIVPCGIADKDVTSLRRETEEVLPSEAVVEE 186
           IGAIGVRIS+GITSHGLAFNVDPDL YFKHIVPCGI DKDVTSLR+ET+EVLP E V+EE
Sbjct: 145 IGAIGVRISNGITSHGLAFNVDPDLMYFKHIVPCGIVDKDVTSLRKETKEVLPGEEVLEE 204

Query: 187 QLISCFVKLFGYCRTQVEENPSIELDLENRIESLK 222
           QLISCFVKLFGYC+TQ+E+    ELD  + IE+LK
Sbjct: 205 QLISCFVKLFGYCKTQMEKILLTELDFGDEIENLK 239

BLAST of Spg000474 vs. NCBI nr
Match: XP_022154074.1 (octanoyltransferase [Momordica charantia])

HSP 1 Score: 389.4 bits (999), Expect = 2.0e-104
Identity = 191/215 (88.84%), Postives = 200/215 (93.02%), Query Frame = 0

Query: 7   EKLVSARKASKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLGAELHYTERGGDIT 66
           + LV+ARKA KIPDTLLSLQHPPTYTLGKRRTDHNLL+PESDLEKLGAELHYTERGGDIT
Sbjct: 25  QSLVAARKACKIPDTLLSLQHPPTYTLGKRRTDHNLLVPESDLEKLGAELHYTERGGDIT 84

Query: 67  FHGPNQAILYPIISLRDVGIGARKYVEKLELTMIEMACLYGVKARAGEKGETGVWVGDRK 126
           FHGPNQAILYPIISLR+VG GARKYVE LEL+MIEMA LYGVKARAGEKGETGVWVGDRK
Sbjct: 85  FHGPNQAILYPIISLRNVGFGARKYVENLELSMIEMAGLYGVKARAGEKGETGVWVGDRK 144

Query: 127 IGAIGVRISSGITSHGLAFNVDPDLRYFKHIVPCGIADKDVTSLRRETEEVLPSEAVVEE 186
           IGAIGVRIS GITSHGLAFN+DPDL YFKHIVPCGIADKDVTSLRRETE VLP EAV+EE
Sbjct: 145 IGAIGVRISGGITSHGLAFNLDPDLTYFKHIVPCGIADKDVTSLRRETEAVLPGEAVLEE 204

Query: 187 QLISCFVKLFGYCRTQVEENPSIELDLENRIESLK 222
           QLISCFVKLFGY RTQVE NP +ELD   +IESLK
Sbjct: 205 QLISCFVKLFGYGRTQVEVNPPVELDFGAKIESLK 239

BLAST of Spg000474 vs. NCBI nr
Match: XP_008451131.1 (PREDICTED: octanoyltransferase [Cucumis melo] >KAA0042285.1 octanoyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 388.7 bits (997), Expect = 3.5e-104
Identity = 190/215 (88.37%), Postives = 200/215 (93.02%), Query Frame = 0

Query: 7   EKLVSARKASKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLGAELHYTERGGDIT 66
           EKLVSARK+SKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDL KLGAELHYTERGGDIT
Sbjct: 25  EKLVSARKSSKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLAKLGAELHYTERGGDIT 84

Query: 67  FHGPNQAILYPIISLRDVGIGARKYVEKLELTMIEMACLYGVKARAGEKGETGVWVGDRK 126
           FHGPNQ+ILYPIISLRDVG GARKYVEKLE TMIEMACLYGVKARAGEKGETGVWVGDRK
Sbjct: 85  FHGPNQSILYPIISLRDVGFGARKYVEKLEQTMIEMACLYGVKARAGEKGETGVWVGDRK 144

Query: 127 IGAIGVRISSGITSHGLAFNVDPDLRYFKHIVPCGIADKDVTSLRRETEEVLPSEAVVEE 186
           IGAIGVRIS+GITSHGLAFNVDPDL YFKHIVPCGI DKDVTSLR+ET+EVLP E V+EE
Sbjct: 145 IGAIGVRISNGITSHGLAFNVDPDLMYFKHIVPCGIVDKDVTSLRKETKEVLPGEEVLEE 204

Query: 187 QLISCFVKLFGYCRTQVEENPSIELDLENRIESLK 222
           QLISCFVKLFGYC+TQVE+     LD  + IE+LK
Sbjct: 205 QLISCFVKLFGYCKTQVEKILLTGLDFGDEIENLK 239

BLAST of Spg000474 vs. ExPASy Swiss-Prot
Match: Q9SXP7 (Octanoyltransferase LIP2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=LIP2 PE=1 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 1.6e-91
Identity = 160/210 (76.19%), Postives = 183/210 (87.14%), Query Frame = 0

Query: 7   EKLVSARKASKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLGAELHYTERGGDIT 66
           EKLVS RKA +IPDTLLSLQHPPTYTLGKRRTDHNLLIPES+L K+GAELHYT+RGGDIT
Sbjct: 25  EKLVSERKAHQIPDTLLSLQHPPTYTLGKRRTDHNLLIPESELTKIGAELHYTQRGGDIT 84

Query: 67  FHGPNQAILYPIISLRDVGIGARKYVEKLELTMIEMACLYGVKARAGEKGETGVWVGDRK 126
           FHGP+QAILYPIISLR +G GAR YVE LE +MIE A +YGVKARAG K ETGVWVGDRK
Sbjct: 85  FHGPHQAILYPIISLRSIGFGARNYVETLERSMIEFASIYGVKARAGNKCETGVWVGDRK 144

Query: 127 IGAIGVRISSGITSHGLAFNVDPDLRYFKHIVPCGIADKDVTSLRRETEEVLPSEAVVEE 186
           IGAIGVRISSGITSHGLA N+DPD++YF+HIVPCGIADK+VTSLRRET+ +LPSE V+ E
Sbjct: 145 IGAIGVRISSGITSHGLALNIDPDMKYFEHIVPCGIADKEVTSLRRETDTLLPSEEVIHE 204

Query: 187 QLISCFVKLFGYCRTQVEENPSIELDLENR 217
           QL+SC  K F Y     +E+PS+ LD +++
Sbjct: 205 QLVSCLAKAFSYDDVVWKEDPSLILDTQDK 234

BLAST of Spg000474 vs. ExPASy Swiss-Prot
Match: A4J246 (Octanoyltransferase OS=Desulfotomaculum reducens (strain MI-1) OX=349161 GN=lipB PE=3 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 7.7e-38
Identity = 79/192 (41.15%), Postives = 118/192 (61.46%), Query Frame = 0

Query: 7   EKLVSARKASKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLGAELHYTERGGDIT 66
           EKL+  R+ +K+ D +L LQHPPT TLG R   +N+L+PE +L++ G  +  + RGGD+T
Sbjct: 21  EKLLLLRQQNKVEDIMLLLQHPPTLTLGTRENRYNILVPEVELKRQGVNIFKSNRGGDVT 80

Query: 67  FHGPNQAILYPIISLRDVGIGARKYVEKLELTMIEMACLYGVKARAGEKGETGVWVGDRK 126
           +HGP Q + YPI+ L   G   R+YV K+E T I++         + E    GVW+G+ K
Sbjct: 81  YHGPGQIVGYPIVDLNGHGKSIREYVHKIEETFIQLLKEEYDLTASRESKYHGVWLGNEK 140

Query: 127 IGAIGVRISSGITSHGLAFNVDPDLRYFKHIVPCGIADKDVTSLRRETEEVLPSEAVVEE 186
           I AIG  +   +T HG AFNV+ +L +F  I PCGI D+ VTSL++   +    E V  +
Sbjct: 141 ITAIGCAVKRWVTMHGFAFNVNTNLSHFNLINPCGITDRGVTSLQKIFGQPQDMEKVY-K 200

Query: 187 QLISCFVKLFGY 199
           Q+I+ F ++F +
Sbjct: 201 QVITYFSRVFDF 211

BLAST of Spg000474 vs. ExPASy Swiss-Prot
Match: Q5LDM3 (Octanoyltransferase OS=Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343) OX=272559 GN=lipB PE=3 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 2.9e-37
Identity = 81/173 (46.82%), Postives = 114/173 (65.90%), Query Frame = 0

Query: 7   EKLVSARK-ASKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLGAELHYTERGGDI 66
           + LV A++      + ++  +HP  YTLG+   ++N+L+ E  L+ +GA L++ +RGGDI
Sbjct: 25  DALVHAKQNGESYENRIIFCEHPHVYTLGRSGKENNMLLGEEQLKTIGATLYHIDRGGDI 84

Query: 67  TFHGPNQAILYPIISLRDVGIGARKYVEKLELTMIEMACLYGVKARAGEKGETGVWV-GD 126
           T+HGP Q + YPI++L + G+G ++YV  LE  +I +   YGV A   EK  TGVW+ GD
Sbjct: 85  TYHGPGQLVCYPILNLEEFGLGLKEYVHLLEEAVIRVCASYGVVAGRLEKA-TGVWLEGD 144

Query: 127 ----RKIGAIGVRISSGITSHGLAFNVDPDLRYFKHIVPCGIADKDVTSLRRE 174
               RKI AIGVR S  +T HGLA NV+ DLRYF +I PCG  DK VTSL++E
Sbjct: 145 TSRARKICAIGVRSSHYVTMHGLALNVNTDLRYFSYIHPCGFIDKGVTSLQQE 196

BLAST of Spg000474 vs. ExPASy Swiss-Prot
Match: Q64UP8 (Octanoyltransferase OS=Bacteroides fragilis (strain YCH46) OX=295405 GN=lipB PE=3 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 2.9e-37
Identity = 81/173 (46.82%), Postives = 114/173 (65.90%), Query Frame = 0

Query: 7   EKLVSARK-ASKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLGAELHYTERGGDI 66
           + LV A++      + ++  +HP  YTLG+   ++N+L+ E  L+ +GA L++ +RGGDI
Sbjct: 25  DALVHAKQNGESYENRIIFCEHPHVYTLGRSGKENNMLLGEEQLKTIGATLYHIDRGGDI 84

Query: 67  TFHGPNQAILYPIISLRDVGIGARKYVEKLELTMIEMACLYGVKARAGEKGETGVWV-GD 126
           T+HGP Q + YPI++L + G+G ++YV  LE  +I +   YGV A   EK  TGVW+ GD
Sbjct: 85  TYHGPGQLVCYPILNLEEFGLGLKEYVHLLEEAVIRVCASYGVVAGRLEKA-TGVWLEGD 144

Query: 127 ----RKIGAIGVRISSGITSHGLAFNVDPDLRYFKHIVPCGIADKDVTSLRRE 174
               RKI AIGVR S  +T HGLA NV+ DLRYF +I PCG  DK VTSL++E
Sbjct: 145 TSRARKICAIGVRSSHYVTMHGLALNVNTDLRYFSYIHPCGFIDKGVTSLQQE 196

BLAST of Spg000474 vs. ExPASy Swiss-Prot
Match: Q1AT14 (Octanoyltransferase OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1) OX=266117 GN=lipB PE=3 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 5.0e-37
Identity = 91/194 (46.91%), Postives = 119/194 (61.34%), Query Frame = 0

Query: 8   KLVSARKASKIPDTLLSLQHPPTYTLGKRRTD-HNLLIPESDLEKLGAELHYTERGGDIT 67
           +LV  R++ +IPDTLL L+HPP YT+G+   D  NL   E  L  LGAE+ +++RGGD T
Sbjct: 30  ELVRKRRSGEIPDTLLLLEHPPVYTVGRAARDASNLGAGEEYLRSLGAEVFWSDRGGDAT 89

Query: 68  FHGPNQAILYPIISL--RDVGIGARKYVEKLELTMIEMACLYGVKARAGEKGETGVWVGD 127
           FHGP Q + YPII L  RD      +Y+  LE  +I     YG++ R   +  TGVWV  
Sbjct: 90  FHGPGQLVGYPIIRLKVRD----THRYLRDLEEVVIRALAGYGLEGRRHPR-YTGVWVNG 149

Query: 128 RKIGAIGVRISSG-ITSHGLAFNVDPDLRYFKHIVPCGIADKDVTSLRRETEEVLPSEAV 187
            KI AIGV+ SSG I SHG A NV+ DL +F  I PCGI +  VTSL RE    +P  A 
Sbjct: 150 SKICAIGVKFSSGWIASHGFALNVNTDLSWFDRITPCGIREYGVTSLERELGREIPL-AG 209

Query: 188 VEEQLISCFVKLFG 198
           VE +++S F ++ G
Sbjct: 210 VEREIVSGFREVLG 217

BLAST of Spg000474 vs. ExPASy TrEMBL
Match: A0A0A0LZZ4 (Lipoyl(octanoyl) transferase OS=Cucumis sativus OX=3659 GN=Csa_1G605760 PE=3 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 2.6e-105
Identity = 189/215 (87.91%), Postives = 202/215 (93.95%), Query Frame = 0

Query: 7   EKLVSARKASKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLGAELHYTERGGDIT 66
           EKLVSARK+SKIPD LLSLQHPPTYTLGKRRTDHNLLIPESDL KLGAELHY+ERGGDIT
Sbjct: 25  EKLVSARKSSKIPDMLLSLQHPPTYTLGKRRTDHNLLIPESDLAKLGAELHYSERGGDIT 84

Query: 67  FHGPNQAILYPIISLRDVGIGARKYVEKLELTMIEMACLYGVKARAGEKGETGVWVGDRK 126
           FHGPNQ+ILYPIISLRDVG GARKYVEKLE TMIEMACLYGVKA+AGEKGETGVWVGDRK
Sbjct: 85  FHGPNQSILYPIISLRDVGFGARKYVEKLEQTMIEMACLYGVKAKAGEKGETGVWVGDRK 144

Query: 127 IGAIGVRISSGITSHGLAFNVDPDLRYFKHIVPCGIADKDVTSLRRETEEVLPSEAVVEE 186
           IGAIGVRIS+G+TSHGLAFNVDPDL YFKHIVPCGI DKDVTSLR+ET+EVLPSE V+EE
Sbjct: 145 IGAIGVRISNGVTSHGLAFNVDPDLMYFKHIVPCGIVDKDVTSLRKETKEVLPSEEVLEE 204

Query: 187 QLISCFVKLFGYCRTQVEENPSIELDLENRIESLK 222
           QLISCFVKLFGYC+TQVE+ P  ELD  + IE+LK
Sbjct: 205 QLISCFVKLFGYCKTQVEKIPLTELDFGDEIENLK 239

BLAST of Spg000474 vs. ExPASy TrEMBL
Match: A0A5D3DTA6 (Lipoyl(octanoyl) transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold699G00090 PE=3 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 5.8e-105
Identity = 190/215 (88.37%), Postives = 201/215 (93.49%), Query Frame = 0

Query: 7   EKLVSARKASKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLGAELHYTERGGDIT 66
           EKLVSARK+SKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDL KLGAELHYTERGGDIT
Sbjct: 25  EKLVSARKSSKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLAKLGAELHYTERGGDIT 84

Query: 67  FHGPNQAILYPIISLRDVGIGARKYVEKLELTMIEMACLYGVKARAGEKGETGVWVGDRK 126
           FHGPNQ+ILYPIISLRDVG GARKYVEKLE TMIEMACLYGVKARAGEKGETGVWVGDRK
Sbjct: 85  FHGPNQSILYPIISLRDVGFGARKYVEKLEQTMIEMACLYGVKARAGEKGETGVWVGDRK 144

Query: 127 IGAIGVRISSGITSHGLAFNVDPDLRYFKHIVPCGIADKDVTSLRRETEEVLPSEAVVEE 186
           IGAIGVRIS+GITSHGLAFNVDPDL YFKHIVPCGI DKDVTSLR+ET+EVLP E V+EE
Sbjct: 145 IGAIGVRISNGITSHGLAFNVDPDLMYFKHIVPCGIVDKDVTSLRKETKEVLPGEEVLEE 204

Query: 187 QLISCFVKLFGYCRTQVEENPSIELDLENRIESLK 222
           QLISCFVKLFGYC+TQ+E+    ELD  + IE+LK
Sbjct: 205 QLISCFVKLFGYCKTQMEKILLTELDFGDEIENLK 239

BLAST of Spg000474 vs. ExPASy TrEMBL
Match: A0A6J1DKZ5 (Lipoyl(octanoyl) transferase OS=Momordica charantia OX=3673 GN=LOC111021417 PE=3 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 9.8e-105
Identity = 191/215 (88.84%), Postives = 200/215 (93.02%), Query Frame = 0

Query: 7   EKLVSARKASKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLGAELHYTERGGDIT 66
           + LV+ARKA KIPDTLLSLQHPPTYTLGKRRTDHNLL+PESDLEKLGAELHYTERGGDIT
Sbjct: 25  QSLVAARKACKIPDTLLSLQHPPTYTLGKRRTDHNLLVPESDLEKLGAELHYTERGGDIT 84

Query: 67  FHGPNQAILYPIISLRDVGIGARKYVEKLELTMIEMACLYGVKARAGEKGETGVWVGDRK 126
           FHGPNQAILYPIISLR+VG GARKYVE LEL+MIEMA LYGVKARAGEKGETGVWVGDRK
Sbjct: 85  FHGPNQAILYPIISLRNVGFGARKYVENLELSMIEMAGLYGVKARAGEKGETGVWVGDRK 144

Query: 127 IGAIGVRISSGITSHGLAFNVDPDLRYFKHIVPCGIADKDVTSLRRETEEVLPSEAVVEE 186
           IGAIGVRIS GITSHGLAFN+DPDL YFKHIVPCGIADKDVTSLRRETE VLP EAV+EE
Sbjct: 145 IGAIGVRISGGITSHGLAFNLDPDLTYFKHIVPCGIADKDVTSLRRETEAVLPGEAVLEE 204

Query: 187 QLISCFVKLFGYCRTQVEENPSIELDLENRIESLK 222
           QLISCFVKLFGY RTQVE NP +ELD   +IESLK
Sbjct: 205 QLISCFVKLFGYGRTQVEVNPPVELDFGAKIESLK 239

BLAST of Spg000474 vs. ExPASy TrEMBL
Match: A0A5A7TLG4 (Lipoyl(octanoyl) transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold824G00760 PE=3 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 1.7e-104
Identity = 190/215 (88.37%), Postives = 200/215 (93.02%), Query Frame = 0

Query: 7   EKLVSARKASKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLGAELHYTERGGDIT 66
           EKLVSARK+SKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDL KLGAELHYTERGGDIT
Sbjct: 25  EKLVSARKSSKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLAKLGAELHYTERGGDIT 84

Query: 67  FHGPNQAILYPIISLRDVGIGARKYVEKLELTMIEMACLYGVKARAGEKGETGVWVGDRK 126
           FHGPNQ+ILYPIISLRDVG GARKYVEKLE TMIEMACLYGVKARAGEKGETGVWVGDRK
Sbjct: 85  FHGPNQSILYPIISLRDVGFGARKYVEKLEQTMIEMACLYGVKARAGEKGETGVWVGDRK 144

Query: 127 IGAIGVRISSGITSHGLAFNVDPDLRYFKHIVPCGIADKDVTSLRRETEEVLPSEAVVEE 186
           IGAIGVRIS+GITSHGLAFNVDPDL YFKHIVPCGI DKDVTSLR+ET+EVLP E V+EE
Sbjct: 145 IGAIGVRISNGITSHGLAFNVDPDLMYFKHIVPCGIVDKDVTSLRKETKEVLPGEEVLEE 204

Query: 187 QLISCFVKLFGYCRTQVEENPSIELDLENRIESLK 222
           QLISCFVKLFGYC+TQVE+     LD  + IE+LK
Sbjct: 205 QLISCFVKLFGYCKTQVEKILLTGLDFGDEIENLK 239

BLAST of Spg000474 vs. ExPASy TrEMBL
Match: A0A1S3BQW0 (Lipoyl(octanoyl) transferase OS=Cucumis melo OX=3656 GN=LOC103492510 PE=3 SV=1)

HSP 1 Score: 388.7 bits (997), Expect = 1.7e-104
Identity = 190/215 (88.37%), Postives = 200/215 (93.02%), Query Frame = 0

Query: 7   EKLVSARKASKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLGAELHYTERGGDIT 66
           EKLVSARK+SKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDL KLGAELHYTERGGDIT
Sbjct: 25  EKLVSARKSSKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLAKLGAELHYTERGGDIT 84

Query: 67  FHGPNQAILYPIISLRDVGIGARKYVEKLELTMIEMACLYGVKARAGEKGETGVWVGDRK 126
           FHGPNQ+ILYPIISLRDVG GARKYVEKLE TMIEMACLYGVKARAGEKGETGVWVGDRK
Sbjct: 85  FHGPNQSILYPIISLRDVGFGARKYVEKLEQTMIEMACLYGVKARAGEKGETGVWVGDRK 144

Query: 127 IGAIGVRISSGITSHGLAFNVDPDLRYFKHIVPCGIADKDVTSLRRETEEVLPSEAVVEE 186
           IGAIGVRIS+GITSHGLAFNVDPDL YFKHIVPCGI DKDVTSLR+ET+EVLP E V+EE
Sbjct: 145 IGAIGVRISNGITSHGLAFNVDPDLMYFKHIVPCGIVDKDVTSLRKETKEVLPGEEVLEE 204

Query: 187 QLISCFVKLFGYCRTQVEENPSIELDLENRIESLK 222
           QLISCFVKLFGYC+TQVE+     LD  + IE+LK
Sbjct: 205 QLISCFVKLFGYCKTQVEKILLTGLDFGDEIENLK 239

BLAST of Spg000474 vs. TAIR 10
Match: AT1G04640.1 (lipoyltransferase 2 )

HSP 1 Score: 337.0 bits (863), Expect = 1.1e-92
Identity = 160/210 (76.19%), Postives = 183/210 (87.14%), Query Frame = 0

Query: 7   EKLVSARKASKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLGAELHYTERGGDIT 66
           EKLVS RKA +IPDTLLSLQHPPTYTLGKRRTDHNLLIPES+L K+GAELHYT+RGGDIT
Sbjct: 25  EKLVSERKAHQIPDTLLSLQHPPTYTLGKRRTDHNLLIPESELTKIGAELHYTQRGGDIT 84

Query: 67  FHGPNQAILYPIISLRDVGIGARKYVEKLELTMIEMACLYGVKARAGEKGETGVWVGDRK 126
           FHGP+QAILYPIISLR +G GAR YVE LE +MIE A +YGVKARAG K ETGVWVGDRK
Sbjct: 85  FHGPHQAILYPIISLRSIGFGARNYVETLERSMIEFASIYGVKARAGNKCETGVWVGDRK 144

Query: 127 IGAIGVRISSGITSHGLAFNVDPDLRYFKHIVPCGIADKDVTSLRRETEEVLPSEAVVEE 186
           IGAIGVRISSGITSHGLA N+DPD++YF+HIVPCGIADK+VTSLRRET+ +LPSE V+ E
Sbjct: 145 IGAIGVRISSGITSHGLALNIDPDMKYFEHIVPCGIADKEVTSLRRETDTLLPSEEVIHE 204

Query: 187 QLISCFVKLFGYCRTQVEENPSIELDLENR 217
           QL+SC  K F Y     +E+PS+ LD +++
Sbjct: 205 QLVSCLAKAFSYDDVVWKEDPSLILDTQDK 234

BLAST of Spg000474 vs. TAIR 10
Match: AT1G04640.2 (lipoyltransferase 2 )

HSP 1 Score: 337.0 bits (863), Expect = 1.1e-92
Identity = 160/210 (76.19%), Postives = 183/210 (87.14%), Query Frame = 0

Query: 7   EKLVSARKASKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLGAELHYTERGGDIT 66
           EKLVS RKA +IPDTLLSLQHPPTYTLGKRRTDHNLLIPES+L K+GAELHYT+RGGDIT
Sbjct: 25  EKLVSERKAHQIPDTLLSLQHPPTYTLGKRRTDHNLLIPESELTKIGAELHYTQRGGDIT 84

Query: 67  FHGPNQAILYPIISLRDVGIGARKYVEKLELTMIEMACLYGVKARAGEKGETGVWVGDRK 126
           FHGP+QAILYPIISLR +G GAR YVE LE +MIE A +YGVKARAG K ETGVWVGDRK
Sbjct: 85  FHGPHQAILYPIISLRSIGFGARNYVETLERSMIEFASIYGVKARAGNKCETGVWVGDRK 144

Query: 127 IGAIGVRISSGITSHGLAFNVDPDLRYFKHIVPCGIADKDVTSLRRETEEVLPSEAVVEE 186
           IGAIGVRISSGITSHGLA N+DPD++YF+HIVPCGIADK+VTSLRRET+ +LPSE V+ E
Sbjct: 145 IGAIGVRISSGITSHGLALNIDPDMKYFEHIVPCGIADKEVTSLRRETDTLLPSEEVIHE 204

Query: 187 QLISCFVKLFGYCRTQVEENPSIELDLENR 217
           QL+SC  K F Y     +E+PS+ LD +++
Sbjct: 205 QLVSCLAKAFSYDDVVWKEDPSLILDTQDK 234

BLAST of Spg000474 vs. TAIR 10
Match: AT1G47578.1 (Biotin/lipoate A/B protein ligase family )

HSP 1 Score: 132.9 bits (333), Expect = 3.2e-31
Identity = 78/220 (35.45%), Postives = 125/220 (56.82%), Query Frame = 0

Query: 2   KASYGEKLVSARKASKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLGAELHYTER 61
           K+   EK     K  +  D+L+ LQHP  YT+G   +++ L     D++    +++ TER
Sbjct: 67  KSILNEKKALIDKNQECSDSLIILQHPSVYTMGTGSSENYL---NFDIKNAPFDVYRTER 126

Query: 62  GGDITFHGPNQAILYPIISLRDVGIGARKYVEKLELTMIE-MACLYGVKARAGEKGETGV 121
           GG++T+HGP Q ++YPII+LR+  +    Y+ KLE  +I  ++  + + A   + G TGV
Sbjct: 127 GGEVTYHGPGQLVMYPIINLRNHKMDLHWYLRKLEEVVIRVLSSAFAINASRLD-GFTGV 186

Query: 122 WVGDRKIGAIGVRISSGITSHGLAFNVDPDLRYFKHIVPCGIADKDVTSLRRETE----- 181
           WVG++K+ AIG+R+S  +T HGLA NV  DL  F  IVPCGI ++ V S++   E     
Sbjct: 187 WVGNKKMAAIGIRVSKWMTYHGLALNVTTDLTPFNSIVPCGIRNRGVGSVKGLIEDGEHY 246

Query: 182 ---EVLPSEAVVEEQLISCFVKLFGYCRTQVEENPSIELD 213
              E L    +  E L+  F ++F   + Q+E+    +L+
Sbjct: 247 NKLEDLQLLDIAHESLLKEFSEVF---QLQMEKQTVFKLE 279

BLAST of Spg000474 vs. TAIR 10
Match: AT4G31050.1 (Biotin/lipoate A/B protein ligase family )

HSP 1 Score: 131.7 bits (330), Expect = 7.1e-31
Identity = 70/176 (39.77%), Postives = 105/176 (59.66%), Query Frame = 0

Query: 2   KASYGEKLVSARKASKIPDTLLSLQHPPTYTLGKRRTDHNLLIPESDLEKLGAELHYTER 61
           K+   EK     +     DT++ LQH P YT+G   T+  L     D++     ++ TER
Sbjct: 65  KSIVEEKKTLIDRNQDCADTVILLQHSPVYTMGTASTEDYL---NFDIKDAPFNVYRTER 124

Query: 62  GGDITFHGPNQAILYPIISLRDVGIGARKYVEKLELTMIE-MACLYGVKARAGEKGETGV 121
           GG++T+HGP Q ++YPII+LR+  +    Y+  LE  +I  ++  + +KA   + G TGV
Sbjct: 125 GGEVTYHGPGQLVMYPIINLRNHEMDLHWYLRMLEEIVIRVLSSTFSIKASRLD-GLTGV 184

Query: 122 WVGDRKIGAIGVRISSGITSHGLAFNVDPDLRYFKHIVPCGIADKDVTSLRRETEE 177
           WVG++K+ AIG+R+S  IT HGLA NV  DL  F  IVPCGI D+ V +++   E+
Sbjct: 185 WVGNQKVAAIGIRVSKWITYHGLALNVTTDLTPFNWIVPCGIRDRKVGNIKGLLED 236

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038879976.14.7e-10992.89octanoyltransferase LIP2, mitochondrial [Benincasa hispida] >XP_038879977.1 octa... [more]
KAE8653496.14.1e-10588.37hypothetical protein Csa_007704 [Cucumis sativus][more]
TYK26906.11.2e-10488.37octanoyltransferase [Cucumis melo var. makuwa][more]
XP_022154074.12.0e-10488.84octanoyltransferase [Momordica charantia][more]
XP_008451131.13.5e-10488.37PREDICTED: octanoyltransferase [Cucumis melo] >KAA0042285.1 octanoyltransferase ... [more]
Match NameE-valueIdentityDescription
Q9SXP71.6e-9176.19Octanoyltransferase LIP2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=LIP2 ... [more]
A4J2467.7e-3841.15Octanoyltransferase OS=Desulfotomaculum reducens (strain MI-1) OX=349161 GN=lipB... [more]
Q5LDM32.9e-3746.82Octanoyltransferase OS=Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM ... [more]
Q64UP82.9e-3746.82Octanoyltransferase OS=Bacteroides fragilis (strain YCH46) OX=295405 GN=lipB PE=... [more]
Q1AT145.0e-3746.91Octanoyltransferase OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / ... [more]
Match NameE-valueIdentityDescription
A0A0A0LZZ42.6e-10587.91Lipoyl(octanoyl) transferase OS=Cucumis sativus OX=3659 GN=Csa_1G605760 PE=3 SV=... [more]
A0A5D3DTA65.8e-10588.37Lipoyl(octanoyl) transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A6J1DKZ59.8e-10588.84Lipoyl(octanoyl) transferase OS=Momordica charantia OX=3673 GN=LOC111021417 PE=3... [more]
A0A5A7TLG41.7e-10488.37Lipoyl(octanoyl) transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A1S3BQW01.7e-10488.37Lipoyl(octanoyl) transferase OS=Cucumis melo OX=3656 GN=LOC103492510 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G04640.11.1e-9276.19lipoyltransferase 2 [more]
AT1G04640.21.1e-9276.19lipoyltransferase 2 [more]
AT1G47578.13.2e-3135.45Biotin/lipoate A/B protein ligase family [more]
AT4G31050.17.1e-3139.77Biotin/lipoate A/B protein ligase family [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 3..197
e-value: 1.6E-66
score: 225.6
NoneNo IPR availablePANTHERPTHR10993:SF7LIPOYLTRANSFERASE 2, MITOCHONDRIAL-RELATEDcoord: 7..214
NoneNo IPR availablePANTHERPTHR10993OCTANOYLTRANSFERASEcoord: 7..214
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 7..198
IPR004143Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL), catalytic domainPFAMPF03099BPL_LplA_LipBcoord: 41..150
e-value: 2.1E-8
score: 34.2
IPR004143Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL), catalytic domainPROSITEPS51733BPL_LPL_CATALYTICcoord: 16..200
score: 60.087353
IPR000544OctanoyltransferaseTIGRFAMTIGR00214TIGR00214coord: 14..197
e-value: 2.0E-43
score: 146.1
IPR000544OctanoyltransferasePIRSFPIRSF016262LPLasecoord: 2..208
e-value: 2.6E-60
score: 201.4
IPR000544OctanoyltransferaseCDDcd16444LipBcoord: 7..196
e-value: 8.54283E-88
score: 255.879

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg000474.1Spg000474.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009249 protein lipoylation
biological_process GO:0006464 cellular protein modification process
cellular_component GO:0005739 mitochondrion
molecular_function GO:0033819 lipoyl(octanoyl) transferase activity
molecular_function GO:0102555 octanoyl transferase activity (acting on glycine-cleavage complex H protein)