Spg000329 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg000329
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Cysteine protease
Location	scaffold8: 37927754 .. 37930491 (+)
RNA-Seq Expression	Spg000329
Synteny	Spg000329

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTCTCCATTATTTTAACCTTTAATAATTCTGTGAAAATGGTAAAGTGTTTCTCTCTCCTCGTCGCTCTCTCTCTCCTCGCCGTATCGGCGATCGGCGGTGAGATCTTCTCCGGCGATTTGTACGGAGATTCTATAATCCGTCAGGTTGTCGACGAAGGAGGCGTCAACGAAGGAAGTAACGGCGACGATCTGCTACTCGGAGCCGAGCACCACTTTTCGCTCTTTAAGCAGAAGTTTGGGAAGTTGTACGCCTCCAAGGAGGAGCATGATCACCGGTTCAGGGTCTTCAAAGCGAACCTGAGGCGAGCTCAACGCCACCAGGCCCTTGATCCCTCTGCTACTCATGGAGTCACTCAGTTCTCTGATTTGACTCCATCGGAGTTCCGAAGATCTTTTCTAGGGCTTAGAGGTCGTCGTCTCGGGCTCCCGGTTGATGCTAACAAGGCCCCTATTCTTCCCACCGATGGTCTTCCTACCGATTTCGATTGGAGAGATCATGGAGCTGTCACGGAAGTCAAGAATCAGGTTTGGTTTTTTTCCCCTTTTTTTCTCTTTGAATGTAATTTTACCGTCATTTTTCATCTAAATTTTGATTCGGGGAGAAGAGTCTTCTTCGTCTAATTTTGTTATGGAATTTAATATTTTCCGATTCTCCTTCGTATCGCTTATATCTCTGTTCTTTATAATAAATAAGTATCAAACATCGCTATTTCGTTTATGTTCGCAACTTTTTACTCGTACTATGTGTTTGTTATTCCTTATGTTGATGTTGACTTTCAATTAATTCCTTCAGGGAACAAAGATGACATTTATTTATTAAACCGAGGTTTTGATTGTTGTGATTGTTTTTGTTTTATAAGGGTTCGTGTGGATCGTGCTGGAGTTTCAGTACAACCGGTGCTCTTGAAGGCGCTAACTTCCTATCTACCGGCAAACTTGTTAGCCTTAGCGAACAGCAGCTTGTTGACTGTGATCACGAGGTTTGGATTTTAATTTTCACGTCTTTGATCTGCTGTTCTTTAAGTGAATTGTGCAGAGTTTACGTTTAGATAATGATCGTTTATGGTTTTGTATTGGGTGTCATATCTATCTCTTTGCAACATGATTCCAGAGATTTAGAAAACAAAATGCCTATTTTTGCGTTGGTCGATATATGATTTTAGTTTATGTTGATAGTTGCCTTCAATTAGATTTAGATACTACGCGTTCATTTGTTACTGCTACTGTGTACTGTGGAGACAATTGCTAAATCTTTGCGTCTGTATAGTATGTATTAGCTGCCAATAGAAGATCTGTACTAGAAAATTTGGCAGACTTGAAAAGGAATAGAGTGCAAGGTTTGTCCTTGCAGTTTGCTTGAAGTAATTGATAAATAGTTAACCTTTTGGTAGTCAAAGTGAGCATAGTTCCGTGGTAATTGGTGGCAGGGTTGATTATTGCTTAACATTTATGATTATTTATAGTTATTCTGTATTTCATTCAAACTGAAGATAAATTAGTTTCTGTTCATTAATTATGTGTCGATTATGTCATTCTTATATATTTTGACATTCATGTCATCTGAACATTAAAAATATGCACTTAACTTCATATCCTGACTTTTGTTCACGTATCTACCTGGAATTTCATTGAGAAAGGTGTTTCTTTTCATTTTCATTCAAATTAAAGAACTTCTATACTTTTGCCAGTGTGATCCAGAAGAAAAAGGTTCCTGTGATTCTGGCTGCAATGGGGGGTTAATGAACAGTGCATTCGAGTACACATTGAAAGCCGGTGGACTTATGAAAGAGAAAGACTACCCATACACTGGTACAGATCGTGGAACCTGCAAGTTTGACAAGAGCAAAATTGCTGCAACAGTGGCCAACTTTAGTGTTGTCTCCCTTGATGAAGAACAAATTGCTGCCAATCTCGTGAAAAATGGCCCTCTTGCAAGTAATTGCATTCAACCCAGCCTTCTTAAATCCTGTTCTTTTCTTCTATTTGTATAACCAACTATAGTTGCTGACTACTCAATTTCTCTTGAATGCAGTTGCTATCAATGCTGTATTCATGCAGACCTATATAGGAGGGGTTTCTTGCCCTTACATTTGCTCAAAGCATTTGGATCATGGAGTTCTGTTGGTTGGTTATGGATCTGCTGGCTATGCTCCCATCAGATTGAAAGAGAAACCCTACTGGATCATTAAGAACTCCTGGGGAGCCAACTGGGGTGAGAATGGATACTACAAAATCTGCAGGGGTCGCAATATCTGTGGAGTCGATTCCATGGTGTCTACAGTCGCCGCAGTTCACACCGCCTCGAACTAGTTTATGAACTCAAAACTGTTGGAATACCACCAGCTACAATTCCTGTATATGTTGCAATATTATCATTCTATGGCAGGAAGTTGTTAACTGCCATTTCAAGCATAGGTTGTCTCTTTAAAAGTAGAATTTGAACTCTCTGCCTTGTGTTGGTTTAGAAACTTGAAAAAGTAGCTTATTATATATGCATATTTATATTTAGATCAAGCTGTTATGGAGAAGAATTGCTTGTAAGCAAAAAACAAGAATCTGTCTATACGATTTGTGGTTGGCTCACCTACTCTACTTGATGATGCCAATGAAAAATTGTACCAATGTAAATGTCAACATGATAGCGAAAGATGCTTTCTGATGTGTTATCTCAATCTTTTCTGATGCTAAACGTCATTCATGATAATGACAATTGGTCTTAGCATTGTTTTAATGCTTA

mRNA sequence

ATGGTAAAGTGTTTCTCTCTCCTCGTCGCTCTCTCTCTCCTCGCCGTATCGGCGATCGGCGGTGAGATCTTCTCCGGCGATTTGTACGGAGATTCTATAATCCGTCAGGTTGTCGACGAAGGAGGCGTCAACGAAGGAAGTAACGGCGACGATCTGCTACTCGGAGCCGAGCACCACTTTTCGCTCTTTAAGCAGAAGTTTGGGAAGTTGTACGCCTCCAAGGAGGAGCATGATCACCGGTTCAGGGTCTTCAAAGCGAACCTGAGGCGAGCTCAACGCCACCAGGCCCTTGATCCCTCTGCTACTCATGGAGTCACTCAGTTCTCTGATTTGACTCCATCGGAGTTCCGAAGATCTTTTCTAGGGCTTAGAGGTCGTCGTCTCGGGCTCCCGGTTGATGCTAACAAGGCCCCTATTCTTCCCACCGATGGTCTTCCTACCGATTTCGATTGGAGAGATCATGGAGCTGTCACGGAAGTCAAGAATCAGGGTTCGTGTGGATCGTGCTGGAGTTTCAGTACAACCGGTGCTCTTGAAGGCGCTAACTTCCTATCTACCGGCAAACTTGTTAGCCTTAGCGAACAGCAGCTTGTTGACTGTGATCACGAGTGTGATCCAGAAGAAAAAGGTTCCTGTGATTCTGGCTGCAATGGGGGGTTAATGAACAGTGCATTCGAGTACACATTGAAAGCCGGTGGACTTATGAAAGAGAAAGACTACCCATACACTGGTACAGATCGTGGAACCTGCAAGTTTGACAAGAGCAAAATTGCTGCAACAGTGGCCAACTTTAGTGTTGTCTCCCTTGATGAAGAACAAATTGCTGCCAATCTCGTGAAAAATGGCCCTCTTGCAATTGCTATCAATGCTGTATTCATGCAGACCTATATAGGAGGGGTTTCTTGCCCTTACATTTGCTCAAAGCATTTGGATCATGGAGTTCTGTTGGTTGGTTATGGATCTGCTGGCTATGCTCCCATCAGATTGAAAGAGAAACCCTACTGGATCATTAAGAACTCCTGGGGAGCCAACTGGGGTGAGAATGGATACTACAAAATCTGCAGGGGTCGCAATATCTGTGGAGTCGATTCCATGGTGTCTACAGTCGCCGCAGTTCACACCGCCTCGAACTAG

Coding sequence (CDS)

ATGGTAAAGTGTTTCTCTCTCCTCGTCGCTCTCTCTCTCCTCGCCGTATCGGCGATCGGCGGTGAGATCTTCTCCGGCGATTTGTACGGAGATTCTATAATCCGTCAGGTTGTCGACGAAGGAGGCGTCAACGAAGGAAGTAACGGCGACGATCTGCTACTCGGAGCCGAGCACCACTTTTCGCTCTTTAAGCAGAAGTTTGGGAAGTTGTACGCCTCCAAGGAGGAGCATGATCACCGGTTCAGGGTCTTCAAAGCGAACCTGAGGCGAGCTCAACGCCACCAGGCCCTTGATCCCTCTGCTACTCATGGAGTCACTCAGTTCTCTGATTTGACTCCATCGGAGTTCCGAAGATCTTTTCTAGGGCTTAGAGGTCGTCGTCTCGGGCTCCCGGTTGATGCTAACAAGGCCCCTATTCTTCCCACCGATGGTCTTCCTACCGATTTCGATTGGAGAGATCATGGAGCTGTCACGGAAGTCAAGAATCAGGGTTCGTGTGGATCGTGCTGGAGTTTCAGTACAACCGGTGCTCTTGAAGGCGCTAACTTCCTATCTACCGGCAAACTTGTTAGCCTTAGCGAACAGCAGCTTGTTGACTGTGATCACGAGTGTGATCCAGAAGAAAAAGGTTCCTGTGATTCTGGCTGCAATGGGGGGTTAATGAACAGTGCATTCGAGTACACATTGAAAGCCGGTGGACTTATGAAAGAGAAAGACTACCCATACACTGGTACAGATCGTGGAACCTGCAAGTTTGACAAGAGCAAAATTGCTGCAACAGTGGCCAACTTTAGTGTTGTCTCCCTTGATGAAGAACAAATTGCTGCCAATCTCGTGAAAAATGGCCCTCTTGCAATTGCTATCAATGCTGTATTCATGCAGACCTATATAGGAGGGGTTTCTTGCCCTTACATTTGCTCAAAGCATTTGGATCATGGAGTTCTGTTGGTTGGTTATGGATCTGCTGGCTATGCTCCCATCAGATTGAAAGAGAAACCCTACTGGATCATTAAGAACTCCTGGGGAGCCAACTGGGGTGAGAATGGATACTACAAAATCTGCAGGGGTCGCAATATCTGTGGAGTCGATTCCATGGTGTCTACAGTCGCCGCAGTTCACACCGCCTCGAACTAG

Protein sequence

MVKCFSLLVALSLLAVSAIGGEIFSGDLYGDSIIRQVVDEGGVNEGSNGDDLLLGAEHHFSLFKQKFGKLYASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRSFLGLRGRRLGLPVDANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGANFLSTGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKAGGLMKEKDYPYTGTDRGTCKFDKSKIAATVANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGGVSCPYICSKHLDHGVLLVGYGSAGYAPIRLKEKPYWIIKNSWGANWGENGYYKICRGRNICGVDSMVSTVAAVHTASN

Homology

[Close]

BLAST of Spg000329 vs. NCBI nr
Match: ARA15252.1 (cysteine proteinase 2 [Citrullus lanatus])

HSP 1 Score: 737.3 bits (1902), Expect = 6.8e-209
Identity = 357/377 (94.69%), Postives = 366/377 (97.08%), Query Frame = 0


Query: 1   MVKCFSLLVALSLLAVSAIGGEIFSGDLYGDSIIRQVVDEGGVNEGSNGDDLLLGAEHHF 60
           MVKCFSL+VALSLLAVSAIG E+FSG+  GD +IRQVVD+GGVN GSNGDDLLLGAEHHF
Sbjct: 1   MVKCFSLIVALSLLAVSAIGAEVFSGESDGDFVIRQVVDDGGVNGGSNGDDLLLGAEHHF 60

Query: 61  SLFKQKFGKLYASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120
           SLFK+KFGK YASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRSF
Sbjct: 61  SLFKRKFGKSYASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120

Query: 121 LGLRGRRLGLPVDANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEG 180
           LGLRGRRLGLP DANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEG
Sbjct: 121 LGLRGRRLGLPADANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEG 180

Query: 181 ANFLSTGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKAGGLMKEKDY 240
           ANFL+TGKLVSLSEQQLVDCDHECDPEEK SCDSGCNGGLMNSAFEYTLKAGGLMKE DY
Sbjct: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMKENDY 240

Query: 241 PYTGTDRGTCKFDKSKIAATVANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGGV 300
           PYTGTDRGTCKFDKSKIAA+VANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGGV
Sbjct: 241 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGGV 300

Query: 301 SCPYICSKHLDHGVLLVGYGSAGYAPIRLKEKPYWIIKNSWGANWGENGYYKICRGRNIC 360
           SCPYICSKHLDHGVLLVGYGS  YAPIRLKEKPYWIIKNSWGANWGENGYYKICRGRNIC
Sbjct: 301 SCPYICSKHLDHGVLLVGYGSGAYAPIRLKEKPYWIIKNSWGANWGENGYYKICRGRNIC 360

Query: 361 GVDSMVSTVAAVHTASN 378
           GVDSMVSTVAAVHTA+N
Sbjct: 361 GVDSMVSTVAAVHTAAN 377

[Close]

BLAST of Spg000329 vs. NCBI nr
Match: XP_023006920.1 (cysteine protease RD19A-like [Cucurbita maxima])

HSP 1 Score: 725.7 bits (1872), Expect = 2.0e-205
Identity = 352/378 (93.12%), Postives = 365/378 (96.56%), Query Frame = 0


Query: 1   MVKCFSLLVALSLLAVSAIGGEIFSGDLYGDSIIRQVVDEGGVN-EGSNGDDLLLGAEHH 60
           MVKCFSL VALSLLAVSAIGG+ FSGD YGDSIIRQVVD+GGVN  GSNGDDLLLGAEHH
Sbjct: 1   MVKCFSLFVALSLLAVSAIGGDHFSGDGYGDSIIRQVVDDGGVNGGGSNGDDLLLGAEHH 60

Query: 61  FSLFKQKFGKLYASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRS 120
           FS+FKQKFGK YASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRR 
Sbjct: 61  FSVFKQKFGKSYASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRL 120

Query: 121 FLGLRGRRLGLPVDANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALE 180
           FLGLRGRRLGLPVDANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFS+TGALE
Sbjct: 121 FLGLRGRRLGLPVDANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSSTGALE 180

Query: 181 GANFLSTGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKAGGLMKEKD 240
           GANFLS+G+LVSLSEQQLVDCDHECDPEEKGSCD+GC+GGLMNSAFEYTLKAGGLMKEKD
Sbjct: 181 GANFLSSGELVSLSEQQLVDCDHECDPEEKGSCDAGCSGGLMNSAFEYTLKAGGLMKEKD 240

Query: 241 YPYTGTDRGTCKFDKSKIAATVANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGG 300
           YPYTGTDRG CKFDK+KIAA+VANFSV+SLDEEQIAANLVKNGPLAI INAVFMQTYIGG
Sbjct: 241 YPYTGTDRGACKFDKTKIAASVANFSVISLDEEQIAANLVKNGPLAIGINAVFMQTYIGG 300

Query: 301 VSCPYICSKHLDHGVLLVGYGSAGYAPIRLKEKPYWIIKNSWGANWGENGYYKICRGRNI 360
           VSCPYICSKHLDHGVLLVGYGSA YAPIR+KEKPYWIIKNSWGA WGENGYYK+CRGRNI
Sbjct: 301 VSCPYICSKHLDHGVLLVGYGSAAYAPIRMKEKPYWIIKNSWGAKWGENGYYKLCRGRNI 360

Query: 361 CGVDSMVSTVAAVHTASN 378
           CGVDSMVSTVAAVH  SN
Sbjct: 361 CGVDSMVSTVAAVHITSN 378

[Close]

BLAST of Spg000329 vs. NCBI nr
Match: XP_022948236.1 (cysteine protease RD19A-like [Cucurbita moschata])

HSP 1 Score: 723.0 bits (1865), Expect = 1.3e-204
Identity = 350/378 (92.59%), Postives = 364/378 (96.30%), Query Frame = 0


Query: 1   MVKCFSLLVALSLLAVSAIGGEIFSGDLYGDSIIRQVVDEGGVN-EGSNGDDLLLGAEHH 60
           MVKCFSL VALSLLAVSAIGG+ FSGD YGDSIIRQVVD+GGVN  GSNGDDLLLGAEHH
Sbjct: 26  MVKCFSLFVALSLLAVSAIGGDHFSGDGYGDSIIRQVVDDGGVNGGGSNGDDLLLGAEHH 85

Query: 61  FSLFKQKFGKLYASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRS 120
           FS+FKQKFGK YAS EEHDHRFR+FKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRR 
Sbjct: 86  FSVFKQKFGKSYASMEEHDHRFRIFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRL 145

Query: 121 FLGLRGRRLGLPVDANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALE 180
           FLGLRGRRLGLP+DANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFS TGALE
Sbjct: 146 FLGLRGRRLGLPLDANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSATGALE 205

Query: 181 GANFLSTGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKAGGLMKEKD 240
           GANFLS+GKLVSLSEQQLVDCDHECDPEE+GSCD+GC+GGLMNSAFEYTLKAGGLMKEKD
Sbjct: 206 GANFLSSGKLVSLSEQQLVDCDHECDPEEQGSCDAGCSGGLMNSAFEYTLKAGGLMKEKD 265

Query: 241 YPYTGTDRGTCKFDKSKIAATVANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGG 300
           YPYTGTDRG CKFDK+KIAA+VANFSV+SLDEEQIAANLVKNGPLAI INAVFMQTYIGG
Sbjct: 266 YPYTGTDRGACKFDKTKIAASVANFSVISLDEEQIAANLVKNGPLAIGINAVFMQTYIGG 325

Query: 301 VSCPYICSKHLDHGVLLVGYGSAGYAPIRLKEKPYWIIKNSWGANWGENGYYKICRGRNI 360
           VSCPYICSKHLDHGVLLVGYGSA YAPIR+KEKPYWIIKNSWGA WGENGYYK+CRGRNI
Sbjct: 326 VSCPYICSKHLDHGVLLVGYGSAAYAPIRMKEKPYWIIKNSWGAKWGENGYYKLCRGRNI 385

Query: 361 CGVDSMVSTVAAVHTASN 378
           CGVDSMVSTVAAVH ASN
Sbjct: 386 CGVDSMVSTVAAVHIASN 403

[Close]

BLAST of Spg000329 vs. NCBI nr
Match: KAG7035065.1 (Cysteine protease RD19A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 723.0 bits (1865), Expect = 1.3e-204
Identity = 350/378 (92.59%), Postives = 364/378 (96.30%), Query Frame = 0


Query: 1   MVKCFSLLVALSLLAVSAIGGEIFSGDLYGDSIIRQVVDEGGVN-EGSNGDDLLLGAEHH 60
           MVKCFSL VALSLLAVSAIGG+ FSGD YGDSIIRQVVD+GGVN  GSNGDDLLLGAEHH
Sbjct: 1   MVKCFSLFVALSLLAVSAIGGDHFSGDGYGDSIIRQVVDDGGVNGGGSNGDDLLLGAEHH 60

Query: 61  FSLFKQKFGKLYASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRS 120
           FS+FKQKFGK YAS EEHDHRFR+FKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRR 
Sbjct: 61  FSVFKQKFGKSYASMEEHDHRFRIFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRL 120

Query: 121 FLGLRGRRLGLPVDANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALE 180
           FLGLRGRRLGLP+DANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFS TGALE
Sbjct: 121 FLGLRGRRLGLPLDANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSATGALE 180

Query: 181 GANFLSTGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKAGGLMKEKD 240
           GANFLS+GKLVSLSEQQLVDCDHECDPEE+GSCD+GC+GGLMNSAFEYTLKAGGLMKEKD
Sbjct: 181 GANFLSSGKLVSLSEQQLVDCDHECDPEEQGSCDAGCSGGLMNSAFEYTLKAGGLMKEKD 240

Query: 241 YPYTGTDRGTCKFDKSKIAATVANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGG 300
           YPYTGTDRG CKFDK+KIAA+VANFSV+SLDEEQIAANLVKNGPLAI INAVFMQTYIGG
Sbjct: 241 YPYTGTDRGACKFDKTKIAASVANFSVISLDEEQIAANLVKNGPLAIGINAVFMQTYIGG 300

Query: 301 VSCPYICSKHLDHGVLLVGYGSAGYAPIRLKEKPYWIIKNSWGANWGENGYYKICRGRNI 360
           VSCPYICSKHLDHGVLLVGYGSA YAPIR+KEKPYWIIKNSWGA WGENGYYK+CRGRNI
Sbjct: 301 VSCPYICSKHLDHGVLLVGYGSAAYAPIRMKEKPYWIIKNSWGAKWGENGYYKLCRGRNI 360

Query: 361 CGVDSMVSTVAAVHTASN 378
           CGVDSMVSTVAAVH ASN
Sbjct: 361 CGVDSMVSTVAAVHIASN 378

[Close]

BLAST of Spg000329 vs. NCBI nr
Match: XP_004150061.1 (cysteine protease RD19A [Cucumis sativus] >KGN61836.1 hypothetical protein Csa_006176 [Cucumis sativus])

HSP 1 Score: 722.6 bits (1864), Expect = 1.7e-204
Identity = 348/377 (92.31%), Postives = 363/377 (96.29%), Query Frame = 0


Query: 1   MVKCFSLLVALSLLAVSAIGGEIFSGDLYGDSIIRQVVDEGGVNEGSNGDDLLLGAEHHF 60
           MVKCFSL+V LSLLA SAIG E+ SG+  GD IIRQVVD+GGVNEGSNGDDLLLGA+HHF
Sbjct: 1   MVKCFSLIVVLSLLAASAIGSEVISGESDGDFIIRQVVDDGGVNEGSNGDDLLLGADHHF 60

Query: 61  SLFKQKFGKLYASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120
           S+FKQKFGK YASKEEHDHRFRVFKANL+RAQRHQALDPSATHGVTQFSDLTPSEFRRSF
Sbjct: 61  SVFKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120

Query: 121 LGLRGRRLGLPVDANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEG 180
           LGLR RRLGLP DANKAPILPTDGLPTDFDWRD GAV+EVKNQGSCGSCWSFS TGALEG
Sbjct: 121 LGLRSRRLGLPADANKAPILPTDGLPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEG 180

Query: 181 ANFLSTGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKAGGLMKEKDY 240
           ANFL+TGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLK+GGLMKE+DY
Sbjct: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDY 240

Query: 241 PYTGTDRGTCKFDKSKIAATVANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGGV 300
           PYTGTDRGTCKFDKSKIAA+VANFSVVSLDEEQIAANLVKNGPLA+AINAVFMQTYI GV
Sbjct: 241 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIKGV 300

Query: 301 SCPYICSKHLDHGVLLVGYGSAGYAPIRLKEKPYWIIKNSWGANWGENGYYKICRGRNIC 360
           SCPYICSKHLDHGVLLVGYGS GYAPIRLK+KPYWIIKNSWGANWGENGYYKICRGRNIC
Sbjct: 301 SCPYICSKHLDHGVLLVGYGSDGYAPIRLKDKPYWIIKNSWGANWGENGYYKICRGRNIC 360

Query: 361 GVDSMVSTVAAVHTASN 378
           GVDSMVSTVAAVHTA+N
Sbjct: 361 GVDSMVSTVAAVHTAAN 377

[Close]

BLAST of Spg000329 vs. ExPASy Swiss-Prot
Match: P43296 (Cysteine protease RD19A OS=Arabidopsis thaliana OX=3702 GN=RD19A PE=1 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 7.9e-160
Identity = 279/370 (75.41%), Postives = 309/370 (83.51%), Query Frame = 0


Query: 2   VKCFSLLVALSLLAVSAIGGEIFSGDLYGDSIIRQVVDEGGVNEGSNGDDLLLGAEHHFS 61
           +K +  +  LS   VS    ++  GD   D +IRQVV  GG       +  +L +E HFS
Sbjct: 4   LKLYFSVFVLSFFIVSVSSSDVNDGD---DLVIRQVV--GG------AEPQVLTSEDHFS 63

Query: 62  LFKQKFGKLYASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRSFL 121
           LFK+KFGK+YAS EEHD+RF VFKANLRRA+RHQ LDPSATHGVTQFSDLT SEFR+  L
Sbjct: 64  LFKRKFGKVYASNEEHDYRFSVFKANLRRARRHQKLDPSATHGVTQFSDLTRSEFRKKHL 123

Query: 122 GLRGRRLGLPVDANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGA 181
           G+R     LP DANKAPILPT+ LP DFDWRDHGAVT VKNQGSCGSCWSFS TGALEGA
Sbjct: 124 GVRS-GFKLPKDANKAPILPTENLPEDFDWRDHGAVTPVKNQGSCGSCWSFSATGALEGA 183

Query: 182 NFLSTGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKAGGLMKEKDYP 241
           NFL+TGKLVSLSEQQLVDCDHECDPEE  SCDSGCNGGLMNSAFEYTLK GGLMKE+DYP
Sbjct: 184 NFLATGKLVSLSEQQLVDCDHECDPEEADSCDSGCNGGLMNSAFEYTLKTGGLMKEEDYP 243

Query: 242 YTGTDRGTCKFDKSKIAATVANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGGVS 301
           YTG D  TCK DKSKI A+V+NFSV+S+DEEQIAANLVKNGPLA+AINA +MQTYIGGVS
Sbjct: 244 YTGKDGKTCKLDKSKIVASVSNFSVISIDEEQIAANLVKNGPLAVAINAGYMQTYIGGVS 303

Query: 302 CPYICSKHLDHGVLLVGYGSAGYAPIRLKEKPYWIIKNSWGANWGENGYYKICRGRNICG 361
           CPYIC++ L+HGVLLVGYG+AGYAP R KEKPYWIIKNSWG  WGENG+YKIC+GRNICG
Sbjct: 304 CPYICTRRLNHGVLLVGYGAAGYAPARFKEKPYWIIKNSWGETWGENGFYKICKGRNICG 361

Query: 362 VDSMVSTVAA 372
           VDSMVSTVAA
Sbjct: 364 VDSMVSTVAA 361

[Close]

BLAST of Spg000329 vs. ExPASy Swiss-Prot
Match: P43295 (Probable cysteine protease RD19B OS=Arabidopsis thaliana OX=3702 GN=RD19B PE=2 SV=2)

HSP 1 Score: 548.9 bits (1413), Expect = 4.5e-155
Identity = 268/367 (73.02%), Postives = 307/367 (83.65%), Query Frame = 0


Query: 5   FSLLVALSLLAVSAIGGEIFSGDLYGDSIIRQVVDEGGVNEGSNGDDLLLGAEHHFSLFK 64
           FS+ +    ++VS  G E        D +IRQVVDE         +  +L +E HF+LFK
Sbjct: 9   FSVSLIFVFVSVSVCGDE--------DVLIRQVVDE--------TEPKVLSSEDHFTLFK 68

Query: 65  QKFGKLYASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRSFLGLR 124
           +KFGK+Y S EEH +RF VFKANL RA RHQ +DPSA HGVTQFSDLT SEFRR  LG++
Sbjct: 69  KKFGKVYGSIEEHYYRFSVFKANLLRAMRHQKMDPSARHGVTQFSDLTRSEFRRKHLGVK 128

Query: 125 GRRLGLPVDANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGANFL 184
           G    LP DAN+APILPT  LP +FDWRD GAVT VKNQGSCGSCWSFSTTGALEGA+FL
Sbjct: 129 G-GFKLPKDANQAPILPTQNLPEEFDWRDRGAVTPVKNQGSCGSCWSFSTTGALEGAHFL 188

Query: 185 STGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKAGGLMKEKDYPYTG 244
           +TGKLVSLSEQQLVDCDHECDPEE+GSCDSGCNGGLMNSAFEYTLK GGLM+EKDYPYTG
Sbjct: 189 ATGKLVSLSEQQLVDCDHECDPEEEGSCDSGCNGGLMNSAFEYTLKTGGLMREKDYPYTG 248

Query: 245 TDRGTCKFDKSKIAATVANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGGVSCPY 304
           TD G+CK D+SKI A+V+NFSVVS++E+QIAANL+KNGPLA+AINA +MQTYIGGVSCPY
Sbjct: 249 TDGGSCKLDRSKIVASVSNFSVVSINEDQIAANLIKNGPLAVAINAAYMQTYIGGVSCPY 308

Query: 305 ICSKHLDHGVLLVGYGSAGYAPIRLKEKPYWIIKNSWGANWGENGYYKICRGRNICGVDS 364
           ICS+ L+HGVLLVGYGSAG++  RLKEKPYWIIKNSWG +WGENG+YKIC+GRNICGVDS
Sbjct: 309 ICSRRLNHGVLLVGYGSAGFSQARLKEKPYWIIKNSWGESWGENGFYKICKGRNICGVDS 358

Query: 365 MVSTVAA 372
           +VSTVAA
Sbjct: 369 LVSTVAA 358

[Close]

BLAST of Spg000329 vs. ExPASy Swiss-Prot
Match: Q9SUL1 (Probable cysteine protease RD19C OS=Arabidopsis thaliana OX=3702 GN=RD19C PE=2 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 1.3e-154
Identity = 269/374 (71.93%), Postives = 309/374 (82.62%), Query Frame = 0


Query: 5   FSLLVALSLLAVSAIGGEIFSGDLYGDSI--IRQVVDEGGVNEGSNGDDLLLGAEHHFSL 64
           F  L+A +LLA  ++G  + SG++    +  IRQVV E         D+ LL AEHHF+L
Sbjct: 6   FFFLIAATLLA-GSLGSTVISGEVTDGFVNPIRQVVPE-------ENDEQLLNAEHHFTL 65

Query: 65  FKQKFGKLYASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRSFLG 124
           FK K+ K YA++ EHDHRFRVFKANLRRA+R+Q LDPSA HGVTQFSDLTP EFRR FLG
Sbjct: 66  FKSKYEKTYATQVEHDHRFRVFKANLRRARRNQLLDPSAVHGVTQFSDLTPKEFRRKFLG 125

Query: 125 LRGRRLGLPVDANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGAN 184
           L+ R   LP D   APILPT  LPT+FDWR+ GAVT VKNQG CGSCWSFS  GALEGA+
Sbjct: 126 LKRRGFRLPTDTQTAPILPTSDLPTEFDWREQGAVTPVKNQGMCGSCWSFSAIGALEGAH 185

Query: 185 FLSTGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKAGGLMKEKDYPY 244
           FL+T +LVSLSEQQLVDCDHECDP +  SCDSGC+GGLMN+AFEY LKAGGLMKE+DYPY
Sbjct: 186 FLATKELVSLSEQQLVDCDHECDPAQANSCDSGCSGGLMNNAFEYALKAGGLMKEEDYPY 245

Query: 245 TGTDRGTCKFDKSKIAATVANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGGVSC 304
           TG D   CKFDKSKI A+V+NFSVVS DE+QIAANLV++GPLAIAINA++MQTYIGGVSC
Sbjct: 246 TGRDHTACKFDKSKIVASVSNFSVVSSDEDQIAANLVQHGPLAIAINAMWMQTYIGGVSC 305

Query: 305 PYICSKHLDHGVLLVGYGSAGYAPIRLKEKPYWIIKNSWGANWGENGYYKICRG-RNICG 364
           PY+CSK  DHGVLLVG+GS+GYAPIRLKEKPYWIIKNSWGA WGE+GYYKICRG  N+CG
Sbjct: 306 PYVCSKSQDHGVLLVGFGSSGYAPIRLKEKPYWIIKNSWGAMWGEHGYYKICRGPHNMCG 365

Query: 365 VDSMVSTVAAVHTA 376
           +D+MVSTVAAVHT+
Sbjct: 366 MDTMVSTVAAVHTS 371

[Close]

BLAST of Spg000329 vs. ExPASy Swiss-Prot
Match: P25804 (Cysteine proteinase 15A OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 2.4e-148
Identity = 256/364 (70.33%), Postives = 299/364 (82.14%), Query Frame = 0


Query: 12  SLLAVSAIGGEIFSGDLYGDSIIRQVVDEGGVNEGSNGDDLLLGAEHHFSLFKQKFGKLY 71
           +L   +A+   +       D IIRQVVD        N +D LL AEHHF+ FK KF K Y
Sbjct: 8   ALFLFAAVATAVTDDTNNDDFIIRQVVD--------NEEDHLLNAEHHFTSFKSKFSKSY 67

Query: 72  ASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRSFLGLRGRRLGLP 131
           A+KEEHD+RF VFK+NL +A+ HQ  DP+A HG+T+FSDLT SEFRR FLGL+ +RL LP
Sbjct: 68  ATKEEHDYRFGVFKSNLIKAKLHQNRDPTAEHGITKFSDLTASEFRRQFLGLK-KRLRLP 127

Query: 132 VDANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGANFLSTGKLVS 191
             A KAPILPT  LP DFDWR+ GAVT VK+QGSCGSCW+FSTTGALEGA++L+TGKLVS
Sbjct: 128 AHAQKAPILPTTNLPEDFDWREKGAVTPVKDQGSCGSCWAFSTTGALEGAHYLATGKLVS 187

Query: 192 LSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKAGGLMKEKDYPYTGTDRGTCK 251
           LSEQQLVDCDH CDPE+ GSCDSGCNGGLMN+AFEY L++GG+++EKDY YTG D G+CK
Sbjct: 188 LSEQQLVDCDHVCDPEQAGSCDSGCNGGLMNNAFEYLLESGGVVQEKDYAYTGRD-GSCK 247

Query: 252 FDKSKIAATVANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGGVSCPYICSK-HL 311
           FDKSK+ A+V+NFSVV+LDE+QIAANLVKNGPLA+AINA +MQTY+ GVSCPY+C+K  L
Sbjct: 248 FDKSKVVASVSNFSVVTLDEDQIAANLVKNGPLAVAINAAWMQTYMSGVSCPYVCAKSRL 307

Query: 312 DHGVLLVGYGSAGYAPIRLKEKPYWIIKNSWGANWGENGYYKICRGRNICGVDSMVSTVA 371
           DHGVLLVG+G   YAPIRLKEKPYWIIKNSWG NWGE GYYKICRGRN+CGVDSMVSTVA
Sbjct: 308 DHGVLLVGFGKGAYAPIRLKEKPYWIIKNSWGQNWGEQGYYKICRGRNVCGVDSMVSTVA 361

Query: 372 AVHT 375
           A  +
Sbjct: 368 AAQS 361

[Close]

BLAST of Spg000329 vs. ExPASy Swiss-Prot
Match: Q10716 (Cysteine proteinase 1 OS=Zea mays OX=4577 GN=CCP1 PE=2 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 2.1e-144
Identity = 256/376 (68.09%), Postives = 296/376 (78.72%), Query Frame = 0


Query: 7   LLVALSLLAVSAIGGEIFSGDLYGDSIIRQVVDEGGVNEGSNGDDLLLGAEHHFSLFKQK 66
           +L+ LSL + +A+   + +     D +IRQVV       G + +DL L AE HF  F Q+
Sbjct: 5   VLLLLSLASAAAVAAAVDA----EDPLIRQVV------PGGDDNDLELNAESHFLSFVQR 64

Query: 67  FGKLYASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRSFLGLRGR 126
           FGK Y   +EH +R  VFK NLRRA+RHQ LDPSA HGVT+FSDLTP+EFRR++LGLR  
Sbjct: 65  FGKSYKDADEHAYRLSVFKDNLRRARRHQLLDPSAEHGVTKFSDLTPAEFRRTYLGLRKS 124

Query: 127 RLG----LPVDANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGAN 186
           R      L   A++AP+LPTDGLP DFDWRDHGAV  VKNQGSCGSCWSFS +GALEGA+
Sbjct: 125 RRALLRELGESAHEAPVLPTDGLPDDFDWRDHGAVGPVKNQGSCGSCWSFSASGALEGAH 184

Query: 187 FLSTGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKAGGLMKEKDYPY 246
           +L+TGKL  LSEQQ VDCDHECD  E  SCDSGCNGGLM +AF Y  KAGGL  EKDYPY
Sbjct: 185 YLATGKLEVLSEQQFVDCDHECDSSEPDSCDSGCNGGLMTTAFSYLQKAGGLESEKDYPY 244

Query: 247 TGTDRGTCKFDKSKIAATVANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGGVSC 306
           TG+D G CKFDKSKI A+V NFSVVS+DE QI+ANL+K+GPLAI INA +MQTYIGGVSC
Sbjct: 245 TGSD-GKCKFDKSKIVASVQNFSVVSVDEAQISANLIKHGPLAIGINAAYMQTYIGGVSC 304

Query: 307 PYICSKHLDHGVLLVGYGSAGYAPIRLKEKPYWIIKNSWGANWGENGYYKICRG---RNI 366
           PYIC +HLDHGVLLVGYG++G+APIRLK+KPYWIIKNSWG NWGENGYYKICRG   RN 
Sbjct: 305 PYICGRHLDHGVLLVGYGASGFAPIRLKDKPYWIIKNSWGENWGENGYYKICRGSNVRNK 364

Query: 367 CGVDSMVSTVAAVHTA 376
           CGVDSMVSTV+AVH +
Sbjct: 365 CGVDSMVSTVSAVHAS 369

[Close]

BLAST of Spg000329 vs. ExPASy TrEMBL
Match: A0A7R6B0V1 (Cysteine proteinase 2 OS=Citrullus lanatus OX=3654 PE=2 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 3.3e-209
Identity = 357/377 (94.69%), Postives = 366/377 (97.08%), Query Frame = 0


Query: 1   MVKCFSLLVALSLLAVSAIGGEIFSGDLYGDSIIRQVVDEGGVNEGSNGDDLLLGAEHHF 60
           MVKCFSL+VALSLLAVSAIG E+FSG+  GD +IRQVVD+GGVN GSNGDDLLLGAEHHF
Sbjct: 1   MVKCFSLIVALSLLAVSAIGAEVFSGESDGDFVIRQVVDDGGVNGGSNGDDLLLGAEHHF 60

Query: 61  SLFKQKFGKLYASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120
           SLFK+KFGK YASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRSF
Sbjct: 61  SLFKRKFGKSYASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120

Query: 121 LGLRGRRLGLPVDANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEG 180
           LGLRGRRLGLP DANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEG
Sbjct: 121 LGLRGRRLGLPADANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEG 180

Query: 181 ANFLSTGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKAGGLMKEKDY 240
           ANFL+TGKLVSLSEQQLVDCDHECDPEEK SCDSGCNGGLMNSAFEYTLKAGGLMKE DY
Sbjct: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKDSCDSGCNGGLMNSAFEYTLKAGGLMKENDY 240

Query: 241 PYTGTDRGTCKFDKSKIAATVANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGGV 300
           PYTGTDRGTCKFDKSKIAA+VANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGGV
Sbjct: 241 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGGV 300

Query: 301 SCPYICSKHLDHGVLLVGYGSAGYAPIRLKEKPYWIIKNSWGANWGENGYYKICRGRNIC 360
           SCPYICSKHLDHGVLLVGYGS  YAPIRLKEKPYWIIKNSWGANWGENGYYKICRGRNIC
Sbjct: 301 SCPYICSKHLDHGVLLVGYGSGAYAPIRLKEKPYWIIKNSWGANWGENGYYKICRGRNIC 360

Query: 361 GVDSMVSTVAAVHTASN 378
           GVDSMVSTVAAVHTA+N
Sbjct: 361 GVDSMVSTVAAVHTAAN 377

[Close]

BLAST of Spg000329 vs. ExPASy TrEMBL
Match: A0A6J1L1J1 (cysteine protease RD19A-like OS=Cucurbita maxima OX=3661 GN=LOC111499563 PE=3 SV=1)

HSP 1 Score: 725.7 bits (1872), Expect = 9.9e-206
Identity = 352/378 (93.12%), Postives = 365/378 (96.56%), Query Frame = 0


Query: 1   MVKCFSLLVALSLLAVSAIGGEIFSGDLYGDSIIRQVVDEGGVN-EGSNGDDLLLGAEHH 60
           MVKCFSL VALSLLAVSAIGG+ FSGD YGDSIIRQVVD+GGVN  GSNGDDLLLGAEHH
Sbjct: 1   MVKCFSLFVALSLLAVSAIGGDHFSGDGYGDSIIRQVVDDGGVNGGGSNGDDLLLGAEHH 60

Query: 61  FSLFKQKFGKLYASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRS 120
           FS+FKQKFGK YASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRR 
Sbjct: 61  FSVFKQKFGKSYASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRL 120

Query: 121 FLGLRGRRLGLPVDANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALE 180
           FLGLRGRRLGLPVDANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFS+TGALE
Sbjct: 121 FLGLRGRRLGLPVDANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSSTGALE 180

Query: 181 GANFLSTGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKAGGLMKEKD 240
           GANFLS+G+LVSLSEQQLVDCDHECDPEEKGSCD+GC+GGLMNSAFEYTLKAGGLMKEKD
Sbjct: 181 GANFLSSGELVSLSEQQLVDCDHECDPEEKGSCDAGCSGGLMNSAFEYTLKAGGLMKEKD 240

Query: 241 YPYTGTDRGTCKFDKSKIAATVANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGG 300
           YPYTGTDRG CKFDK+KIAA+VANFSV+SLDEEQIAANLVKNGPLAI INAVFMQTYIGG
Sbjct: 241 YPYTGTDRGACKFDKTKIAASVANFSVISLDEEQIAANLVKNGPLAIGINAVFMQTYIGG 300

Query: 301 VSCPYICSKHLDHGVLLVGYGSAGYAPIRLKEKPYWIIKNSWGANWGENGYYKICRGRNI 360
           VSCPYICSKHLDHGVLLVGYGSA YAPIR+KEKPYWIIKNSWGA WGENGYYK+CRGRNI
Sbjct: 301 VSCPYICSKHLDHGVLLVGYGSAAYAPIRMKEKPYWIIKNSWGAKWGENGYYKLCRGRNI 360

Query: 361 CGVDSMVSTVAAVHTASN 378
           CGVDSMVSTVAAVH  SN
Sbjct: 361 CGVDSMVSTVAAVHITSN 378

[Close]

BLAST of Spg000329 vs. ExPASy TrEMBL
Match: A0A6J1G8Q4 (cysteine protease RD19A-like OS=Cucurbita moschata OX=3662 GN=LOC111451872 PE=3 SV=1)

HSP 1 Score: 723.0 bits (1865), Expect = 6.4e-205
Identity = 350/378 (92.59%), Postives = 364/378 (96.30%), Query Frame = 0


Query: 1   MVKCFSLLVALSLLAVSAIGGEIFSGDLYGDSIIRQVVDEGGVN-EGSNGDDLLLGAEHH 60
           MVKCFSL VALSLLAVSAIGG+ FSGD YGDSIIRQVVD+GGVN  GSNGDDLLLGAEHH
Sbjct: 26  MVKCFSLFVALSLLAVSAIGGDHFSGDGYGDSIIRQVVDDGGVNGGGSNGDDLLLGAEHH 85

Query: 61  FSLFKQKFGKLYASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRS 120
           FS+FKQKFGK YAS EEHDHRFR+FKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRR 
Sbjct: 86  FSVFKQKFGKSYASMEEHDHRFRIFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRL 145

Query: 121 FLGLRGRRLGLPVDANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALE 180
           FLGLRGRRLGLP+DANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFS TGALE
Sbjct: 146 FLGLRGRRLGLPLDANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSATGALE 205

Query: 181 GANFLSTGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKAGGLMKEKD 240
           GANFLS+GKLVSLSEQQLVDCDHECDPEE+GSCD+GC+GGLMNSAFEYTLKAGGLMKEKD
Sbjct: 206 GANFLSSGKLVSLSEQQLVDCDHECDPEEQGSCDAGCSGGLMNSAFEYTLKAGGLMKEKD 265

Query: 241 YPYTGTDRGTCKFDKSKIAATVANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGG 300
           YPYTGTDRG CKFDK+KIAA+VANFSV+SLDEEQIAANLVKNGPLAI INAVFMQTYIGG
Sbjct: 266 YPYTGTDRGACKFDKTKIAASVANFSVISLDEEQIAANLVKNGPLAIGINAVFMQTYIGG 325

Query: 301 VSCPYICSKHLDHGVLLVGYGSAGYAPIRLKEKPYWIIKNSWGANWGENGYYKICRGRNI 360
           VSCPYICSKHLDHGVLLVGYGSA YAPIR+KEKPYWIIKNSWGA WGENGYYK+CRGRNI
Sbjct: 326 VSCPYICSKHLDHGVLLVGYGSAAYAPIRMKEKPYWIIKNSWGAKWGENGYYKLCRGRNI 385

Query: 361 CGVDSMVSTVAAVHTASN 378
           CGVDSMVSTVAAVH ASN
Sbjct: 386 CGVDSMVSTVAAVHIASN 403

[Close]

BLAST of Spg000329 vs. ExPASy TrEMBL
Match: A0A0A0LPD0 (Cysteine protease OS=Cucumis sativus OX=3659 GN=Csa_2G249900 PE=3 SV=1)

HSP 1 Score: 722.6 bits (1864), Expect = 8.4e-205
Identity = 348/377 (92.31%), Postives = 363/377 (96.29%), Query Frame = 0


Query: 1   MVKCFSLLVALSLLAVSAIGGEIFSGDLYGDSIIRQVVDEGGVNEGSNGDDLLLGAEHHF 60
           MVKCFSL+V LSLLA SAIG E+ SG+  GD IIRQVVD+GGVNEGSNGDDLLLGA+HHF
Sbjct: 1   MVKCFSLIVVLSLLAASAIGSEVISGESDGDFIIRQVVDDGGVNEGSNGDDLLLGADHHF 60

Query: 61  SLFKQKFGKLYASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120
           S+FKQKFGK YASKEEHDHRFRVFKANL+RAQRHQALDPSATHGVTQFSDLTPSEFRRSF
Sbjct: 61  SVFKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120

Query: 121 LGLRGRRLGLPVDANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEG 180
           LGLR RRLGLP DANKAPILPTDGLPTDFDWRD GAV+EVKNQGSCGSCWSFS TGALEG
Sbjct: 121 LGLRSRRLGLPADANKAPILPTDGLPTDFDWRDKGAVSEVKNQGSCGSCWSFSATGALEG 180

Query: 181 ANFLSTGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKAGGLMKEKDY 240
           ANFL+TGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLK+GGLMKE+DY
Sbjct: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDY 240

Query: 241 PYTGTDRGTCKFDKSKIAATVANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGGV 300
           PYTGTDRGTCKFDKSKIAA+VANFSVVSLDEEQIAANLVKNGPLA+AINAVFMQTYI GV
Sbjct: 241 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIKGV 300

Query: 301 SCPYICSKHLDHGVLLVGYGSAGYAPIRLKEKPYWIIKNSWGANWGENGYYKICRGRNIC 360
           SCPYICSKHLDHGVLLVGYGS GYAPIRLK+KPYWIIKNSWGANWGENGYYKICRGRNIC
Sbjct: 301 SCPYICSKHLDHGVLLVGYGSDGYAPIRLKDKPYWIIKNSWGANWGENGYYKICRGRNIC 360

Query: 361 GVDSMVSTVAAVHTASN 378
           GVDSMVSTVAAVHTA+N
Sbjct: 361 GVDSMVSTVAAVHTAAN 377

[Close]

BLAST of Spg000329 vs. ExPASy TrEMBL
Match: A0A5D3BWN5 (Cysteine proteinase RD19a-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold280G00440 PE=3 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 6.0e-203
Identity = 345/377 (91.51%), Postives = 361/377 (95.76%), Query Frame = 0


Query: 1   MVKCFSLLVALSLLAVSAIGGEIFSGDLYGDSIIRQVVDEGGVNEGSNGDDLLLGAEHHF 60
           MVKCFSL++ALSLLA SAIG E+ SG+  GD IIRQVVD+GGVN  SNGDDLLLGAEHHF
Sbjct: 1   MVKCFSLIIALSLLAASAIGKEVISGESDGDFIIRQVVDDGGVNGASNGDDLLLGAEHHF 60

Query: 61  SLFKQKFGKLYASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120
           S+FKQKFGK YASKEEHDHRFRVFKANL+RAQRHQALDPSATHGVTQFSDLTPSEFRRSF
Sbjct: 61  SVFKQKFGKSYASKEEHDHRFRVFKANLKRAQRHQALDPSATHGVTQFSDLTPSEFRRSF 120

Query: 121 LGLRGRRLGLPVDANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEG 180
           LGLR RRLGLP DANKAPILPTDGLP DFDWRD GAVTEVKNQGSCGSCWSFS TGALEG
Sbjct: 121 LGLRSRRLGLPEDANKAPILPTDGLPADFDWRDKGAVTEVKNQGSCGSCWSFSATGALEG 180

Query: 181 ANFLSTGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKAGGLMKEKDY 240
           ANFL+TGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLK+GGLMKE+DY
Sbjct: 181 ANFLATGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKSGGLMKEQDY 240

Query: 241 PYTGTDRGTCKFDKSKIAATVANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGGV 300
           PYTGTDRGTCKFDKSKIAA+VANFSVVSLDEEQIAANLVKNGPLA+AINAVFMQTYI GV
Sbjct: 241 PYTGTDRGTCKFDKSKIAASVANFSVVSLDEEQIAANLVKNGPLAVAINAVFMQTYIRGV 300

Query: 301 SCPYICSKHLDHGVLLVGYGSAGYAPIRLKEKPYWIIKNSWGANWGENGYYKICRGRNIC 360
           SCPYICS+HLDHGVLLVGYGS GYAPIR+K+KPYWIIKNSWGANWGENGYYKICRGRNIC
Sbjct: 301 SCPYICSRHLDHGVLLVGYGSEGYAPIRMKDKPYWIIKNSWGANWGENGYYKICRGRNIC 360

Query: 361 GVDSMVSTVAAVHTASN 378
           GVDSMVSTVAAVHTA+N
Sbjct: 361 GVDSMVSTVAAVHTAAN 377

[Close]

BLAST of Spg000329 vs. TAIR 10
Match: AT4G39090.1 (Papain family cysteine protease )

HSP 1 Score: 564.7 bits (1454), Expect = 5.6e-161
Identity = 279/370 (75.41%), Postives = 309/370 (83.51%), Query Frame = 0


Query: 2   VKCFSLLVALSLLAVSAIGGEIFSGDLYGDSIIRQVVDEGGVNEGSNGDDLLLGAEHHFS 61
           +K +  +  LS   VS    ++  GD   D +IRQVV  GG       +  +L +E HFS
Sbjct: 4   LKLYFSVFVLSFFIVSVSSSDVNDGD---DLVIRQVV--GG------AEPQVLTSEDHFS 63

Query: 62  LFKQKFGKLYASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRSFL 121
           LFK+KFGK+YAS EEHD+RF VFKANLRRA+RHQ LDPSATHGVTQFSDLT SEFR+  L
Sbjct: 64  LFKRKFGKVYASNEEHDYRFSVFKANLRRARRHQKLDPSATHGVTQFSDLTRSEFRKKHL 123

Query: 122 GLRGRRLGLPVDANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGA 181
           G+R     LP DANKAPILPT+ LP DFDWRDHGAVT VKNQGSCGSCWSFS TGALEGA
Sbjct: 124 GVRS-GFKLPKDANKAPILPTENLPEDFDWRDHGAVTPVKNQGSCGSCWSFSATGALEGA 183

Query: 182 NFLSTGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKAGGLMKEKDYP 241
           NFL+TGKLVSLSEQQLVDCDHECDPEE  SCDSGCNGGLMNSAFEYTLK GGLMKE+DYP
Sbjct: 184 NFLATGKLVSLSEQQLVDCDHECDPEEADSCDSGCNGGLMNSAFEYTLKTGGLMKEEDYP 243

Query: 242 YTGTDRGTCKFDKSKIAATVANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGGVS 301
           YTG D  TCK DKSKI A+V+NFSV+S+DEEQIAANLVKNGPLA+AINA +MQTYIGGVS
Sbjct: 244 YTGKDGKTCKLDKSKIVASVSNFSVISIDEEQIAANLVKNGPLAVAINAGYMQTYIGGVS 303

Query: 302 CPYICSKHLDHGVLLVGYGSAGYAPIRLKEKPYWIIKNSWGANWGENGYYKICRGRNICG 361
           CPYIC++ L+HGVLLVGYG+AGYAP R KEKPYWIIKNSWG  WGENG+YKIC+GRNICG
Sbjct: 304 CPYICTRRLNHGVLLVGYGAAGYAPARFKEKPYWIIKNSWGETWGENGFYKICKGRNICG 361

Query: 362 VDSMVSTVAA 372
           VDSMVSTVAA
Sbjct: 364 VDSMVSTVAA 361

[Close]

BLAST of Spg000329 vs. TAIR 10
Match: AT2G21430.1 (Papain family cysteine protease )

HSP 1 Score: 548.9 bits (1413), Expect = 3.2e-156
Identity = 268/367 (73.02%), Postives = 307/367 (83.65%), Query Frame = 0


Query: 5   FSLLVALSLLAVSAIGGEIFSGDLYGDSIIRQVVDEGGVNEGSNGDDLLLGAEHHFSLFK 64
           FS+ +    ++VS  G E        D +IRQVVDE         +  +L +E HF+LFK
Sbjct: 9   FSVSLIFVFVSVSVCGDE--------DVLIRQVVDE--------TEPKVLSSEDHFTLFK 68

Query: 65  QKFGKLYASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRSFLGLR 124
           +KFGK+Y S EEH +RF VFKANL RA RHQ +DPSA HGVTQFSDLT SEFRR  LG++
Sbjct: 69  KKFGKVYGSIEEHYYRFSVFKANLLRAMRHQKMDPSARHGVTQFSDLTRSEFRRKHLGVK 128

Query: 125 GRRLGLPVDANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGANFL 184
           G    LP DAN+APILPT  LP +FDWRD GAVT VKNQGSCGSCWSFSTTGALEGA+FL
Sbjct: 129 G-GFKLPKDANQAPILPTQNLPEEFDWRDRGAVTPVKNQGSCGSCWSFSTTGALEGAHFL 188

Query: 185 STGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKAGGLMKEKDYPYTG 244
           +TGKLVSLSEQQLVDCDHECDPEE+GSCDSGCNGGLMNSAFEYTLK GGLM+EKDYPYTG
Sbjct: 189 ATGKLVSLSEQQLVDCDHECDPEEEGSCDSGCNGGLMNSAFEYTLKTGGLMREKDYPYTG 248

Query: 245 TDRGTCKFDKSKIAATVANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGGVSCPY 304
           TD G+CK D+SKI A+V+NFSVVS++E+QIAANL+KNGPLA+AINA +MQTYIGGVSCPY
Sbjct: 249 TDGGSCKLDRSKIVASVSNFSVVSINEDQIAANLIKNGPLAVAINAAYMQTYIGGVSCPY 308

Query: 305 ICSKHLDHGVLLVGYGSAGYAPIRLKEKPYWIIKNSWGANWGENGYYKICRGRNICGVDS 364
           ICS+ L+HGVLLVGYGSAG++  RLKEKPYWIIKNSWG +WGENG+YKIC+GRNICGVDS
Sbjct: 309 ICSRRLNHGVLLVGYGSAGFSQARLKEKPYWIIKNSWGESWGENGFYKICKGRNICGVDS 358

Query: 365 MVSTVAA 372
           +VSTVAA
Sbjct: 369 LVSTVAA 358

[Close]

BLAST of Spg000329 vs. TAIR 10
Match: AT4G16190.1 (Papain family cysteine protease )

HSP 1 Score: 547.4 bits (1409), Expect = 9.3e-156
Identity = 269/374 (71.93%), Postives = 309/374 (82.62%), Query Frame = 0


Query: 5   FSLLVALSLLAVSAIGGEIFSGDLYGDSI--IRQVVDEGGVNEGSNGDDLLLGAEHHFSL 64
           F  L+A +LLA  ++G  + SG++    +  IRQVV E         D+ LL AEHHF+L
Sbjct: 6   FFFLIAATLLA-GSLGSTVISGEVTDGFVNPIRQVVPE-------ENDEQLLNAEHHFTL 65

Query: 65  FKQKFGKLYASKEEHDHRFRVFKANLRRAQRHQALDPSATHGVTQFSDLTPSEFRRSFLG 124
           FK K+ K YA++ EHDHRFRVFKANLRRA+R+Q LDPSA HGVTQFSDLTP EFRR FLG
Sbjct: 66  FKSKYEKTYATQVEHDHRFRVFKANLRRARRNQLLDPSAVHGVTQFSDLTPKEFRRKFLG 125

Query: 125 LRGRRLGLPVDANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGAN 184
           L+ R   LP D   APILPT  LPT+FDWR+ GAVT VKNQG CGSCWSFS  GALEGA+
Sbjct: 126 LKRRGFRLPTDTQTAPILPTSDLPTEFDWREQGAVTPVKNQGMCGSCWSFSAIGALEGAH 185

Query: 185 FLSTGKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKAGGLMKEKDYPY 244
           FL+T +LVSLSEQQLVDCDHECDP +  SCDSGC+GGLMN+AFEY LKAGGLMKE+DYPY
Sbjct: 186 FLATKELVSLSEQQLVDCDHECDPAQANSCDSGCSGGLMNNAFEYALKAGGLMKEEDYPY 245

Query: 245 TGTDRGTCKFDKSKIAATVANFSVVSLDEEQIAANLVKNGPLAIAINAVFMQTYIGGVSC 304
           TG D   CKFDKSKI A+V+NFSVVS DE+QIAANLV++GPLAIAINA++MQTYIGGVSC
Sbjct: 246 TGRDHTACKFDKSKIVASVSNFSVVSSDEDQIAANLVQHGPLAIAINAMWMQTYIGGVSC 305

Query: 305 PYICSKHLDHGVLLVGYGSAGYAPIRLKEKPYWIIKNSWGANWGENGYYKICRG-RNICG 364
           PY+CSK  DHGVLLVG+GS+GYAPIRLKEKPYWIIKNSWGA WGE+GYYKICRG  N+CG
Sbjct: 306 PYVCSKSQDHGVLLVGFGSSGYAPIRLKEKPYWIIKNSWGAMWGEHGYYKICRGPHNMCG 365

Query: 365 VDSMVSTVAAVHTA 376
           +D+MVSTVAAVHT+
Sbjct: 366 MDTMVSTVAAVHTS 371

[Close]

BLAST of Spg000329 vs. TAIR 10
Match: AT3G54940.2 (Papain family cysteine protease )

HSP 1 Score: 429.9 bits (1104), Expect = 2.2e-120
Identity = 209/343 (60.93%), Postives = 259/343 (75.51%), Query Frame = 0


Query: 31  DSIIRQVVDEGGVNEGSNGDDLLLGAEHHFSLFKQKFGKLYASKEEHDHRFRVFKANLRR 90
           D  IRQV  +   N     + L    E  F LF   +GK Y+++EE+ HR  +F  N+ +
Sbjct: 25  DLTIRQVTAD---NRRIRPNLLGTHTESKFRLFMSDYGKNYSTREEYIHRLGIFAKNVLK 84

Query: 91  AQRHQALDPSATHGVTQFSDLTPSEFRRSFLGLR--GRRLGLPVDANKAPILPTDGLPTD 150
           A  HQ +DPSA HGVTQFSDLT  EF+R + G+   G   G  V A +AP++  DGLP D
Sbjct: 85  AAEHQMMDPSAVHGVTQFSDLTEEEFKRMYTGVADVGGSRGGTVGA-EAPMVEVDGLPED 144

Query: 151 FDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGANFLSTGKLVSLSEQQLVDCDHECDPEE 210
           FDWR+ G VTEVKNQG+CGSCW+FSTTGA EGA+F+STGKL+SLSEQQLVDCD  CDP++
Sbjct: 145 FDWREKGGVTEVKNQGACGSCWAFSTTGAAEGAHFVSTGKLLSLSEQQLVDCDQACDPKD 204

Query: 211 KGSCDSGCNGGLMNSAFEYTLKAGGLMKEKDYPYTGTDRGTCKFDKSKIAATVANFSVVS 270
           K +CD+GC GGLM +A+EY ++AGGL +E+ YPYTG  RG CKFD  K+A  V NF+ + 
Sbjct: 205 KKACDNGCGGGLMTNAYEYLMEAGGLEEERSYPYTG-KRGHCKFDPEKVAVRVLNFTTIP 264

Query: 271 LDEEQIAANLVKNGPLAIAINAVFMQTYIGGVSCPYICSK-HLDHGVLLVGYGSAGYAPI 330
           LDE QIAANLV++GPLA+ +NAVFMQTYIGGVSCP ICSK +++HGVLLVGYGS G++ +
Sbjct: 265 LDENQIAANLVRHGPLAVGLNAVFMQTYIGGVSCPLICSKRNVNHGVLLVGYGSKGFSIL 324

Query: 331 RLKEKPYWIIKNSWGANWGENGYYKICRGRNICGVDSMVSTVA 371
           RL  KPYWIIKNSWG  WGENGYYK+CRG +ICG++SMVS VA
Sbjct: 325 RLSNKPYWIIKNSWGKKWGENGYYKLCRGHDICGINSMVSAVA 362

[Close]

BLAST of Spg000329 vs. TAIR 10
Match: AT3G19390.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 230.7 bits (587), Expect = 1.9e-60
Identity = 132/310 (42.58%), Postives = 178/310 (57.42%), Query Frame = 0


Query: 69  KLYASKEEHDHRFRVFKANLRRAQRHQALDPSATH--GVTQFSDLTPSEFRRSFLGLRGR 128
           K Y    E + RF +FK NL+  + H ++ P+ T+  G+T+F+DLT  EFR  +L  +  
Sbjct: 52  KNYNGLGEKERRFEIFKDNLKFVEEHSSI-PNRTYEVGLTRFADLTNDEFRAIYLRSKME 111

Query: 129 RLGLPVDANKAPILPTDGLPTDFDWRDHGAVTEVKNQGSCGSCWSFSTTGALEGANFLST 188
           R  +PV   K      D LP   DWR  GAV  VK+QGSCGSCW+FS  GA+EG N + T
Sbjct: 112 RTRVPVKGEKYLYKVGDSLPDAIDWRAKGAVNPVKDQGSCGSCWAFSAIGAVEGINQIKT 171

Query: 189 GKLVSLSEQQLVDCDHECDPEEKGSCDSGCNGGLMNSAFEYTLKAGGLMKEKDYPYTGTD 248
           G+L+SLSEQ+LVDCD         S + GC GGLM+ AF++ ++ GG+  E+DYPY  TD
Sbjct: 172 GELISLSEQELVDCD--------TSYNDGCGGGLMDYAFKFIIENGGIDTEEDYPYIATD 231

Query: 249 RGTCKFDKSKI-AATVANFSVVSLDEEQIAANLVKNGPLAIAINA--VFMQTYIGGVSCP 308
              C  DK      T+  +  V  ++E+     + N P+++AI A     Q Y  GV   
Sbjct: 232 VNVCNSDKKNTRVVTIDGYEDVPQNDEKSLKKALANQPISVAIEAGGRAFQLYTSGVFTG 291

Query: 309 YICSKHLDHGVLLVGYGSAGYAPIRLKEKPYWIIKNSWGANWGENGYYKICRGRNI---- 368
             C   LDHGV+ VGYGS G        + YWI++NSWG+NWGE+GY+K+   RNI    
Sbjct: 292 -TCGTSLDHGVVAVGYGSEG-------GQDYWIVRNSWGSNWGESGYFKL--ERNIKESS 342

The following BLAST results are available for this feature:

BLAST of Spg000329 vs. NCBI nr


Match Name	E-value	Identity	Description
ARA15252.1	6.8e-209	94.69	cysteine proteinase 2 [Citrullus lanatus]	[more]
XP_023006920.1	2.0e-205	93.12	cysteine protease RD19A-like [Cucurbita maxima]	[more]
XP_022948236.1	1.3e-204	92.59	cysteine protease RD19A-like [Cucurbita moschata]	[more]
KAG7035065.1	1.3e-204	92.59	Cysteine protease RD19A [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_004150061.1	1.7e-204	92.31	cysteine protease RD19A [Cucumis sativus] >KGN61836.1 hypothetical protein Csa_0...	[more]

BLAST of Spg000329 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P43296	7.9e-160	75.41	Cysteine protease RD19A OS=Arabidopsis thaliana OX=3702 GN=RD19A PE=1 SV=1	[more]
P43295	4.5e-155	73.02	Probable cysteine protease RD19B OS=Arabidopsis thaliana OX=3702 GN=RD19B PE=2 S...	[more]
Q9SUL1	1.3e-154	71.93	Probable cysteine protease RD19C OS=Arabidopsis thaliana OX=3702 GN=RD19C PE=2 S...	[more]
P25804	2.4e-148	70.33	Cysteine proteinase 15A OS=Pisum sativum OX=3888 PE=2 SV=1	[more]
Q10716	2.1e-144	68.09	Cysteine proteinase 1 OS=Zea mays OX=4577 GN=CCP1 PE=2 SV=1	[more]

BLAST of Spg000329 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A7R6B0V1	3.3e-209	94.69	Cysteine proteinase 2 OS=Citrullus lanatus OX=3654 PE=2 SV=1	[more]
A0A6J1L1J1	9.9e-206	93.12	cysteine protease RD19A-like OS=Cucurbita maxima OX=3661 GN=LOC111499563 PE=3 SV...	[more]
A0A6J1G8Q4	6.4e-205	92.59	cysteine protease RD19A-like OS=Cucurbita moschata OX=3662 GN=LOC111451872 PE=3 ...	[more]
A0A0A0LPD0	8.4e-205	92.31	Cysteine protease OS=Cucumis sativus OX=3659 GN=Csa_2G249900 PE=3 SV=1	[more]
A0A5D3BWN5	6.0e-203	91.51	Cysteine proteinase RD19a-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]

BLAST of Spg000329 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G39090.1	5.6e-161	75.41	Papain family cysteine protease	[more]
AT2G21430.1	3.2e-156	73.02	Papain family cysteine protease	[more]
AT4G16190.1	9.3e-156	71.93	Papain family cysteine protease	[more]
AT3G54940.2	2.2e-120	60.93	Papain family cysteine protease	[more]
AT3G19390.1	1.9e-60	42.58	Granulin repeat cysteine protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000668	Peptidase C1A, papain C-terminal	PRINTS	PR00705	PAPAIN	coord: 312..322 score: 58.8 coord: 163..178 score: 64.12 coord: 334..340 score: 75.14
IPR000668	Peptidase C1A, papain C-terminal	SMART	SM00645	pept_c1	coord: 145..370 e-value: 8.4E-112 score: 387.4
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 145..368 e-value: 5.4E-75 score: 252.2
IPR013201	Cathepsin propeptide inhibitor domain (I29)	SMART	SM00848	Inhibitor_I29_2	coord: 60..116 e-value: 1.2E-22 score: 91.2
IPR013201	Cathepsin propeptide inhibitor domain (I29)	PFAM	PF08246	Inhibitor_I29	coord: 60..116 e-value: 7.2E-12 score: 45.6
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 44..370 e-value: 1.4E-99 score: 335.8
None	No IPR available	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 38..371
None	No IPR available	PANTHER	PTHR12411:SF783	CYSTEINE PROTEASE RD19C-RELATED	coord: 38..371
IPR025660	Cysteine peptidase, histidine active site	PROSITE	PS00639	THIOL_PROTEASE_HIS	coord: 310..320
IPR025661	Cysteine peptidase, asparagine active site	PROSITE	PS00640	THIOL_PROTEASE_ASN	coord: 334..353
IPR000169	Cysteine peptidase, cysteine active site	PROSITE	PS00139	THIOL_PROTEASE_CYS	coord: 163..174
IPR039417	Papain-like cysteine endopeptidase	CDD	cd02248	Peptidase_C1A	coord: 146..369 e-value: 4.38787E-103 score: 301.08
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 58..369

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg000329.1	Spg000329.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005615	extracellular space
cellular_component	GO:0005764	lysosome
molecular_function	GO:0004197	cysteine-type endopeptidase activity
molecular_function	GO:0008234	cysteine-type peptidase activity