Sgr030558 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr030558
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionChloride channel-like family protein
Locationtig00154107: 1637623 .. 1642246 (-)
RNA-Seq ExpressionSgr030558
SyntenySgr030558
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTCAGGAAAGTTTTGGGATTTGCACCATTAAATACGATACAATTGCCGATTCGGTTAGCGTATGACTTCTTCCAATCAACCAATGCGAACCCATCTCATATAAAATTACGTTTCTGGTCAGAAGCGGAAAACGAGACTCCTCTGCAGATAATAGAGCAAGGCAAGGATGTCCACGGCAGCTGCCGTCTCCACCAATGGCGATGAGGAATCCATTATCACTCCCTTGCTCCCTCCCCAGAAAGCAGTTGCCAACTCTTCCTCACAGGTGGCCATTGTTGGTGCTAATGTCTGCCCCATTGAGAGCCTTGACTACGAGTGAGTTGAGTTATTTGTTTGTGTTTTCTTCTTTCCCCCTTTTGGGTTTTTCTCTTTGAATTCTGTATCATGGCTTTGTTTCTGCAAAGCCTGTTTCTGGGTTTGATGAGGAGTAAGCTTGGTTGGTTTCTCTCCATTTGGGCCATTCCTTTTGATTCCCCTTCTGAGGTTTTGTTTTTCAGGGGTTTTGACTCTTCCAATGATGTGGGGTTTGTGAAAAGTTCATAATGTCTTGTTTGTTACAGGATTTTTGACAACGAGTTCTTCATGCAAGATTGGAGGAGCCGCGAGGATTTCCAGATATTTCAATACTTAATTATGAAGTGGCTGTCATGCTTCTTGATCGGTCTGATAATGGGTCTTGTTGGTTTTTTCAACAACCTGGCGGTGGAAAATATTGCAGGCAAGAAGTTTGTGGTCACTTCGAATATGATGCTTGAGGGCAGGTACTGACTTCCCTTTTCTTCTTCTGTGCTTCGTGGGATTTGGTTAAAGTAGGAATATGTTTGATGTTACTAGGAACAGAGAGTTTTGGCTTTTGAACAGAAACTTAATTGGAATTAAGCGAACAGACATGCATGATAATTGGATTAAGCTCTAAGTTTTGGAAAAAAAACTGTGTTAAAGAGAAGTAAAGCTGTTTTCCAAGATATTGATGGGAGTTGAAAGTTCTTTCAGGTATGCGACGGCTTTTCTTGTTTTCTCTGTTTCCAATTTAGTTCTCACCCTATTCGCATCTGTCATTACGGCATCCATCTGCCCCCAAGCAGCCGGCTCAGGCATACCAGAAGTAAAGGCTTATCTGAATGGCGTGGATGCCCCTGGGATATTATTGCCCCGAACGGTGTTAGTGAAGGTAAAACGATTCCTTGTACATTTTTTCTTGAGCTTCAGAAATCTGTAACCTGGTTTCTTGAGTGCTTTACTTTTAGTTGAAGAGTTGCTCCTTTATTTTGAAGTGCCTATAGTGATGCGAAATGCTCCTTTTTCTCTTCATTTCCGTCAATTAACATTACATGTATGGTTGAATATGTTCTTGATTTTGTTGCCATTTTAAGAAAACTATCTATTTTTTTAGAGAATCTGTTGATCATTGCATGTAGTTCTGTAGTTATTCTCTCAACGCCTGCTTAACAATGAGCCGAGTAATTTAGGGTGGCTTCTCTTCTGTGACTTTTTTGGCACGATCTGTCAAGGAGATACAGATCCTTGCTTTCTTTACAACTGATGAGTGGTGGAAGCCTATTCTTATAGGGGCATCATTAAAACAAACAGCAAGTATGAATTGTCTTGCTACTTGTTTGGGTTAGGAATAGGATTCATCAAAATCTTTGATACACCAATACAGTATAGGTTACTTTTATTAATTATGCTGCCTCTCAATTCCATTCCCTACTCTAGTTAAACTGGATACCTGGTTTGAGTACTTTTGTTGTGGCAGACCTTGGAAAGTTCAAGCCTTGAAAAAGCATTCTACTTGTAAAATAGTTGGGGTTTTGAGGGCTTTAAATACCCTAGTTTGGCCTGCTGTGACTGATCCCTCTCTTGAAGCCAGAGTTTCATGAATATATTGGTTTAATTGCAGATTATTGGCAGCATTTCTATAGTCTCCTCGTCATTGATTGTTGGAAAAGCTGGACCTATGGTACATACAGGGGCATGTGTTGCATCCTTGGTAGGTCAGGGTGGTTTCAAAATATTTGGTTTAACATGGAGATCACTGTGCCATCTCAAGAATGATCGAGATAGGCGGGATCTTGTAACATGTGGAGCCGCTGCTGGAATAGCTGCTGCTTTTCGTGCTCCTGTTGGTGGTGTGCTGTTTGCTCTTGAAGAGATGGCATCTTGGTTGTTAAATGGCTGCGTCTCTTTCCCGATTCTTTTTTCCATGCTTCGGTTATGTGTTGTATGTTTTATTTTGTTTATGGTCTCCTTTTATTGTCAAAAAGGCTTGATCTGTTCCGGAATAACACAACAACAACACATTTGCTATATTACCATGTTAAGTATAAATTCCGATGTTTTACTCAATTACATGGTTATATAAAGCGCACAACCCTTTCAAATTAGACTTAGTTTATTATTGCACCATTTTAATTGTCAATCTCTTGACCCAAATATGAAGTATGTACAGCTTTCTTCAGAATATCTGCCAATTATGGCAGAGATAGCAACCATAGGCTGCATAAATTGTTCTTATATAAGTTTGCTATTGCATCGTTAGCATCATTGTGTATTTCTCTACAGTGATTTGAACTGCATGCATTCTGTATCTCAGTAGCTGTGAATGCTGTGTTAGCGTATGTGAATGACCTCCTCGGGCTGATTATTGTCACAGGTGGAGAAGTGCCCTTCTGTGGCGATCATTTTTCACAACGGCTGTAGTCGCTGTGGTATTACGTGCTCTGATTGATGTTTGTTTAAATGGCTTATGTGGATTGTTTGGTAAAGGGGGGCTTATAATATTTGATACCTACTCAGACTCTCCTACATATCACCTTGAAGATCTACCTCCTGTGCTTGCCCTTGCTATAGTTGGGGGCATACTTGGAAGCGTATATAATTTCCTTTTAAATAAAGTTCTTCGCATTTACAACCTCATACATGAGTGAGTTCCCTTTTCCTTCTTTATAAACGTTACATAGTCTTTTAATAAGTTTGGAATCTAGCTTACACTGTGAACCCTTCTCCAGGAAAGGCATGTTTACAAAATTTTTCTGGCTTGCTCTGTCTCGATTTTTACGTCCTGTCTTCTTTTTGGATTACCATGGTTTGCACCATGCCAACCTTGCCCATCGAGTTCTCGAGAAACTTGTCCTACAATAGGCCGATCTGGTAACTTCAAGAAGTTTCGGTGTGCTCCTGGTCACTACAATGATCTTGCAAGTCTCATATTTAACACCAATGATGATGCCATCAAAAATCTCTTTAGCAAGGGCACAGACTCTGAGTTCCAGTTCTCATCAATGCTCACATTTTTCATTACCTGTTTTTCCTTGAGCATTCTCAGTTATGGGACGGTTGTTCCTGCTGGCCTTTTTGTCCCTGTTATTGTGACTGGAGCTTCTTATGGACGTTTCGTTGGAATGGTTGTTGGTCCATACACAAACCTCAACCACGGCTTCTTTGCCATATTGGGTGCCGCTTCATTTCTTGGGGGGTCAATGAGGACAACTGTTTCTCTCTGTGTTATTCTCCTAGAATTGACCAACAACTTGTTGTTGCTGCCTTTAATAATGCTGGTTCTTCTTGTTTCCAAGACTGTAGCTGATGCTTTCAATGGTAATATATATAACCTGATCATGAAAGCGAAAGGGTTCCCTTATCTAGAAGGCCATGTCGAGCCATACATGAGGCAGCTGACTGTCGCTGATGTGTTAACGAGTCCACTTCAACTATTGAATGGCATCGAGAAGGTTCGTAAGGTAGTAAATGTTCTCAAATGGACGAACCATCATGGATTCCCTGTCATTGACGAGCCTCCTTTCTCTGAATTCCCTGTTTTATATGGCCTAATTCTCAGAGCCCATCTTCTTATGTTGTTAAAGAAGAAAGCTTTCTTGTCCATTCCAGCACCAGCACCAGCACCAGAGGGTGAGGATGCCTTTAAGCTTTTCTCAGCTGATGATTTTGTGAAGATGGGTTCGGGCGACATTGATAGGATAGAAGATGTACAGTTGACTGATGAAGAGATGGATATGTACATTGATCTACATCCCTTTGCTAATACTTCACCTTGTACCGTCCTGGAGACAACGTCATTAGCAAAGGCTCTTGAAGTTTTTCGAGAAACTGGTTTAAGACACATGCTGTGATACCCAAGATCCCCGGGGTAAGTAACTTTCTCATGGTTTTTTTAGGCTTGCTATTTCTTTACACAAGGAAGAAAATGTAGTAGAATTGGATGTTGTCGACATGAGTTCTGATATCAAATTGCCTCTTACTGATATCCAACATGATGTTGGACTATAGAGTACCAACCAGCGACCGCTATGAAGTTTAGCTGTAGTCACCCAATCTCGAACCTGAAGTTTTCTAGACATAGAAAAAGAATGACAACCCTTTATTCACTTTCTGAAAGTTTCGATTTTCAGGATGATTTTCGGTTCATGAACTTCATGTCAATACTAACTTGTCCCCCTTATTGCAGACGTCACCTGTGGTTGGCATATTGACTCGGCACGACTTCATGCCAGATTACATTCTGAGTTTGCATCCATTGCTGGAGAAGAGCAGGTGGAAAAGGTTAAGAATCAAATTTCGCCTGAAGAAAAAATTCTTCTAGTGCAGCAGCGTTCGACGTAA

mRNA sequence

ATGAGTCAGGAAAGTTTTGGGATTTGCACCATTAAATACGATACAATTGCCGATTCGGTTAGCAGCAAGGCAAGGATGTCCACGGCAGCTGCCGTCTCCACCAATGGCGATGAGGAATCCATTATCACTCCCTTGCTCCCTCCCCAGAAAGCAGTTGCCAACTCTTCCTCACAGGTGGCCATTGTTGGTGCTAATGTCTGCCCCATTGAGAGCCTTGACTACGAGATTTTTGACAACGAGTTCTTCATGCAAGATTGGAGGAGCCGCGAGGATTTCCAGATATTTCAATACTTAATTATGAAGTGGCTGTCATGCTTCTTGATCGGTCTGATAATGGGTCTTGTTGGTTTTTTCAACAACCTGGCGGTGGAAAATATTGCAGGCAAGAAGTTTGTGGTCACTTCGAATATGATGCTTGAGGGCAGGTATGCGACGGCTTTTCTTGTTTTCTCTGTTTCCAATTTAGTTCTCACCCTATTCGCATCTGTCATTACGGCATCCATCTGCCCCCAAGCAGCCGGCTCAGGCATACCAGAAGTAAAGGCTTATCTGAATGGCGTGGATGCCCCTGGGATATTATTGCCCCGAACGGTGTTAGTGAAGATTATTGGCAGCATTTCTATAGTCTCCTCGTCATTGATTGTTGGAAAAGCTGGACCTATGGTACATACAGGGGCATGTGTTGCATCCTTGGTAGGTCAGGGTGGTTTCAAAATATTTGGTTTAACATGGAGATCACTGTGCCATCTCAAGAATGATCGAGATAGGCGGGATCTTGTAACATGTGGAGCCGCTGCTGGAATAGCTGCTGCTTTTCGTGCTCCTGTTGGTGGTGTGCTGTTTGCTCTTGAAGAGATGGCATCTTGGTGGAGAAGTGCCCTTCTGTGGCGATCATTTTTCACAACGGCTGTAGTCGCTGTGGTATTACGTGCTCTGATTGATGTTTGTTTAAATGGCTTATGTGGATTGTTTGGTAAAGGGGGGCTTATAATATTTGATACCTACTCAGACTCTCCTACATATCACCTTGAAGATCTACCTCCTGTGCTTGCCCTTGCTATAGTTGGGGGCATACTTGGAAGCGAAAGGCATGTTTACAAAATTTTTCTGGCTTGCTCTGTCTCGATTTTTACGTCCTGTCTTCTTTTTGGATTACCATGGTTTGCACCATGCCAACCTTGCCCATCGAGTTCTCGAGAAACTTGTCCTACAATAGGCCGATCTGGTAACTTCAAGAAGTTTCGGTGTGCTCCTGGTCACTACAATGATCTTGCAAGTCTCATATTTAACACCAATGATGATGCCATCAAAAATCTCTTTAGCAAGGGCACAGACTCTGAGTTCCAGTTCTCATCAATGCTCACATTTTTCATTACCTGTTTTTCCTTGAGCATTCTCAGTTATGGGACGGTTGTTCCTGCTGGCCTTTTTGTCCCTGTTATTGTGACTGGAGCTTCTTATGGACGTTTCGTTGGAATGGTTGTTGGTCCATACACAAACCTCAACCACGGCTTCTTTGCCATATTGGGTGCCGCTTCATTTCTTGGGGGGTCAATGAGGACAACTGTTTCTCTCTGTGTTATTCTCCTAGAATTGACCAACAACTTGTTGTTGCTGCCTTTAATAATGCTGGTTCTTCTTGTTTCCAAGACTGTAGCTGATGCTTTCAATGGTAATATATATAACCTGATCATGAAAGCGAAAGGGTTCCCTTATCTAGAAGGCCATGTCGAGCCATACATGAGGCAGCTGACTGTCGCTGATGTGTTAACGAGTCCACTTCAACTATTGAATGGCATCGAGAAGGTTCGTAAGGTAGTAAATGTTCTCAAATGGACGAACCATCATGGATTCCCTGTCATTGACGAGCCTCCTTTCTCTGAATTCCCTGTTTTATATGGCCTAATTCTCAGAGCCCATCTTCTTATGTTGTTAAAGAAGAAAGCTTTCTTGTCCATTCCAGCACCAGCACCAGCACCAGAGGGTGAGGATGCCTTTAAGCTTTTCTCAGCTGATGATTTTGTGAAGATGGGTTCGGGCGACATTGATAGGATAGAAGATGTACAGTTGACTGATGAAGAGATGGATATGTACATTGATCTACATCCCTTTGCTAATACTTCACCTTGTACCGTCCTGGAGACAACGTCATTAGCAAAGGCTCTTGAAGTTTTTCGAGAAACTGACGTCACCTGTGGTTGGCATATTGACTCGGCACGACTTCATGCCAGATTACATTCTGAGTTTGCATCCATTGCTGGAGAAGAGCAGGTGGAAAAGGTTAAGAATCAAATTTCGCCTGAAGAAAAAATTCTTCTAGTGCAGCAGCGTTCGACGTAA

Coding sequence (CDS)

ATGAGTCAGGAAAGTTTTGGGATTTGCACCATTAAATACGATACAATTGCCGATTCGGTTAGCAGCAAGGCAAGGATGTCCACGGCAGCTGCCGTCTCCACCAATGGCGATGAGGAATCCATTATCACTCCCTTGCTCCCTCCCCAGAAAGCAGTTGCCAACTCTTCCTCACAGGTGGCCATTGTTGGTGCTAATGTCTGCCCCATTGAGAGCCTTGACTACGAGATTTTTGACAACGAGTTCTTCATGCAAGATTGGAGGAGCCGCGAGGATTTCCAGATATTTCAATACTTAATTATGAAGTGGCTGTCATGCTTCTTGATCGGTCTGATAATGGGTCTTGTTGGTTTTTTCAACAACCTGGCGGTGGAAAATATTGCAGGCAAGAAGTTTGTGGTCACTTCGAATATGATGCTTGAGGGCAGGTATGCGACGGCTTTTCTTGTTTTCTCTGTTTCCAATTTAGTTCTCACCCTATTCGCATCTGTCATTACGGCATCCATCTGCCCCCAAGCAGCCGGCTCAGGCATACCAGAAGTAAAGGCTTATCTGAATGGCGTGGATGCCCCTGGGATATTATTGCCCCGAACGGTGTTAGTGAAGATTATTGGCAGCATTTCTATAGTCTCCTCGTCATTGATTGTTGGAAAAGCTGGACCTATGGTACATACAGGGGCATGTGTTGCATCCTTGGTAGGTCAGGGTGGTTTCAAAATATTTGGTTTAACATGGAGATCACTGTGCCATCTCAAGAATGATCGAGATAGGCGGGATCTTGTAACATGTGGAGCCGCTGCTGGAATAGCTGCTGCTTTTCGTGCTCCTGTTGGTGGTGTGCTGTTTGCTCTTGAAGAGATGGCATCTTGGTGGAGAAGTGCCCTTCTGTGGCGATCATTTTTCACAACGGCTGTAGTCGCTGTGGTATTACGTGCTCTGATTGATGTTTGTTTAAATGGCTTATGTGGATTGTTTGGTAAAGGGGGGCTTATAATATTTGATACCTACTCAGACTCTCCTACATATCACCTTGAAGATCTACCTCCTGTGCTTGCCCTTGCTATAGTTGGGGGCATACTTGGAAGCGAAAGGCATGTTTACAAAATTTTTCTGGCTTGCTCTGTCTCGATTTTTACGTCCTGTCTTCTTTTTGGATTACCATGGTTTGCACCATGCCAACCTTGCCCATCGAGTTCTCGAGAAACTTGTCCTACAATAGGCCGATCTGGTAACTTCAAGAAGTTTCGGTGTGCTCCTGGTCACTACAATGATCTTGCAAGTCTCATATTTAACACCAATGATGATGCCATCAAAAATCTCTTTAGCAAGGGCACAGACTCTGAGTTCCAGTTCTCATCAATGCTCACATTTTTCATTACCTGTTTTTCCTTGAGCATTCTCAGTTATGGGACGGTTGTTCCTGCTGGCCTTTTTGTCCCTGTTATTGTGACTGGAGCTTCTTATGGACGTTTCGTTGGAATGGTTGTTGGTCCATACACAAACCTCAACCACGGCTTCTTTGCCATATTGGGTGCCGCTTCATTTCTTGGGGGGTCAATGAGGACAACTGTTTCTCTCTGTGTTATTCTCCTAGAATTGACCAACAACTTGTTGTTGCTGCCTTTAATAATGCTGGTTCTTCTTGTTTCCAAGACTGTAGCTGATGCTTTCAATGGTAATATATATAACCTGATCATGAAAGCGAAAGGGTTCCCTTATCTAGAAGGCCATGTCGAGCCATACATGAGGCAGCTGACTGTCGCTGATGTGTTAACGAGTCCACTTCAACTATTGAATGGCATCGAGAAGGTTCGTAAGGTAGTAAATGTTCTCAAATGGACGAACCATCATGGATTCCCTGTCATTGACGAGCCTCCTTTCTCTGAATTCCCTGTTTTATATGGCCTAATTCTCAGAGCCCATCTTCTTATGTTGTTAAAGAAGAAAGCTTTCTTGTCCATTCCAGCACCAGCACCAGCACCAGAGGGTGAGGATGCCTTTAAGCTTTTCTCAGCTGATGATTTTGTGAAGATGGGTTCGGGCGACATTGATAGGATAGAAGATGTACAGTTGACTGATGAAGAGATGGATATGTACATTGATCTACATCCCTTTGCTAATACTTCACCTTGTACCGTCCTGGAGACAACGTCATTAGCAAAGGCTCTTGAAGTTTTTCGAGAAACTGACGTCACCTGTGGTTGGCATATTGACTCGGCACGACTTCATGCCAGATTACATTCTGAGTTTGCATCCATTGCTGGAGAAGAGCAGGTGGAAAAGGTTAAGAATCAAATTTCGCCTGAAGAAAAAATTCTTCTAGTGCAGCAGCGTTCGACGTAA

Protein sequence

MSQESFGICTIKYDTIADSVSSKARMSTAAAVSTNGDEESIITPLLPPQKAVANSSSQVAIVGANVCPIESLDYEIFDNEFFMQDWRSREDFQIFQYLIMKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRYATAFLVFSVSNLVLTLFASVITASICPQAAGSGIPEVKAYLNGVDAPGILLPRTVLVKIIGSISIVSSSLIVGKAGPMVHTGACVASLVGQGGFKIFGLTWRSLCHLKNDRDRRDLVTCGAAAGIAAAFRAPVGGVLFALEEMASWWRSALLWRSFFTTAVVAVVLRALIDVCLNGLCGLFGKGGLIIFDTYSDSPTYHLEDLPPVLALAIVGGILGSERHVYKIFLACSVSIFTSCLLFGLPWFAPCQPCPSSSRETCPTIGRSGNFKKFRCAPGHYNDLASLIFNTNDDAIKNLFSKGTDSEFQFSSMLTFFITCFSLSILSYGTVVPAGLFVPVIVTGASYGRFVGMVVGPYTNLNHGFFAILGAASFLGGSMRTTVSLCVILLELTNNLLLLPLIMLVLLVSKTVADAFNGNIYNLIMKAKGFPYLEGHVEPYMRQLTVADVLTSPLQLLNGIEKVRKVVNVLKWTNHHGFPVIDEPPFSEFPVLYGLILRAHLLMLLKKKAFLSIPAPAPAPEGEDAFKLFSADDFVKMGSGDIDRIEDVQLTDEEMDMYIDLHPFANTSPCTVLETTSLAKALEVFRETDVTCGWHIDSARLHARLHSEFASIAGEEQVEKVKNQISPEEKILLVQQRST
Homology
BLAST of Sgr030558 vs. NCBI nr
Match: KAG7021222.1 (putative chloride channel-like protein CLC-g [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1261.1 bits (3262), Expect = 0.0e+00
Identity = 650/781 (83.23%), Postives = 697/781 (89.24%), Query Frame = 0

Query: 30  AAVSTNGDEESIITPLLPPQKAVANSSSQVAIVGANVCPIESLDYEIFDNEFFMQDWRSR 89
           AA  +NGDEESIITPLL PQK++ NSSSQVA+VGAN+CPIESLDYEIFDN+FFMQDWRSR
Sbjct: 3   AANPSNGDEESIITPLLAPQKSLVNSSSQVALVGANICPIESLDYEIFDNDFFMQDWRSR 62

Query: 90  EDFQIFQYLIMKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRYATAFLV 149
           EDFQIFQYL++KWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRY  AFLV
Sbjct: 63  EDFQIFQYLVIKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRYWMAFLV 122

Query: 150 FSVSNLVLTLFASVITASICPQAAGSGIPEVKAYLNGVDAPGILLPRTVLVKIIGSISIV 209
           FSVSNL+LTLFASVITA ICP AAGSGIPEVKAYLNGVDAPGIL PRT+LVKIIGSI+IV
Sbjct: 123 FSVSNLLLTLFASVITALICPLAAGSGIPEVKAYLNGVDAPGILSPRTLLVKIIGSIAIV 182

Query: 210 SSSLIVGKAGPMVHTGACVASLVGQGGFKIFGLTWRSLCHLKNDRDRRDLVTCGAAAGIA 269
           SSS++VGKAGPMVHTGACVASLVGQG FK+FGLTWR L HLK DRDRRDLVTCGAAAGIA
Sbjct: 183 SSSMVVGKAGPMVHTGACVASLVGQGCFKVFGLTWRWLYHLKKDRDRRDLVTCGAAAGIA 242

Query: 270 AAFRAPVGGVLFALEEMASWWRSALLWRSFFTTAVVAVVLRALIDVCLNGLCGLFGKGGL 329
           AAFRAPVGGVLFA EEMASWWRSALLWR+FFTTA+VAVVLR+LIDVCLNGLCGLFGKGGL
Sbjct: 243 AAFRAPVGGVLFAFEEMASWWRSALLWRAFFTTAIVAVVLRSLIDVCLNGLCGLFGKGGL 302

Query: 330 IIFDTYSDSPTYHLEDLPPVLALAIVGGILGS------------------ERHVYKIFLA 389
           IIFDTYSD P+YHL+DLPPVLALA +GGILGS                  +  VYK+ LA
Sbjct: 303 IIFDTYSDFPSYHLKDLPPVLALAFIGGILGSFYNFLLTKVLRVYNLIHEKGIVYKVLLA 362

Query: 390 CSVSIFTSCLLFGLPWFAPCQPCPSSSRETCPTIGRSGNFKKFRCAPGHYNDLASLIFNT 449
           CSVSIFTSCLLFGLPWFA CQPCPSS+RE CPTIGRSGNFKKF+C+ GHYNDLASLIFNT
Sbjct: 363 CSVSIFTSCLLFGLPWFASCQPCPSSAREICPTIGRSGNFKKFQCSSGHYNDLASLIFNT 422

Query: 450 NDDAIKNLFSKGTDSEFQFSSMLTFFITCFSLSILSYGTVVPAGLFVPVIVTGASYGRFV 509
           NDDAIKNLFSKGTDSEFQFSSMLTFF+TCFSLS+LSYGTV P GLFVPVIVTGASYGRFV
Sbjct: 423 NDDAIKNLFSKGTDSEFQFSSMLTFFVTCFSLSVLSYGTVAPVGLFVPVIVTGASYGRFV 482

Query: 510 GMVVGPYTNLNHGFFAILGAASFLGGSMRTTVSLCVILLELTNNLLLLPLIMLVLLVSKT 569
           GMVVGPYTNL+HGFFAILGAASFLGGSMRTTVSLCVI+LELTNNLLLLPL+MLVLL+SKT
Sbjct: 483 GMVVGPYTNLSHGFFAILGAASFLGGSMRTTVSLCVIMLELTNNLLLLPLVMLVLLISKT 542

Query: 570 VADAFNGNIYNLIMKAKGFPYLEGHVEPYMRQLTVADVLTSPLQLLNGIEKVRKVVNVLK 629
           VADAFN NIYN IMKAKGFPYLEGHVEPYMRQLTVA VLTSPLQL  GIEKVR VVNVLK
Sbjct: 543 VADAFNCNIYNQIMKAKGFPYLEGHVEPYMRQLTVASVLTSPLQLFRGIEKVRNVVNVLK 602

Query: 630 WTNHHGFPVIDEPPFSEFPVLYGLILRAHLLMLLKKKAFLSIPAPAPAPEGEDAFKLFSA 689
           WT+HHGFP+IDEPPFSEFPVLYGLILRAHL++LLKKKAFLS+  P    E +DA KL SA
Sbjct: 603 WTSHHGFPIIDEPPFSEFPVLYGLILRAHLIVLLKKKAFLSV--PTLGLERQDALKLLSA 662

Query: 690 DDFVKMGSGDIDRIEDVQLTDEEMDMYIDLHPFANTSPCTVLETTSLAKALEVFRET--- 749
           DDF  MGSGD+DRIED+QLTDEEM+M+IDLHPFANTSPCTVLET SLAKA  +FRET   
Sbjct: 663 DDFAMMGSGDVDRIEDIQLTDEEMEMFIDLHPFANTSPCTVLETMSLAKAFAIFRETGLR 722

Query: 750 -----------DVTCGWHIDSARLHARLHSEFASIAGEEQVEKVKNQISPEEKILLVQQR 779
                       +  G +IDSARLHA LHSEFASIAGEEQ+EKVKNQIS EEKILLV  +
Sbjct: 723 HMLVIPKVPGVKIARGRYIDSARLHAGLHSEFASIAGEEQMEKVKNQISSEEKILLVDHQ 781

BLAST of Sgr030558 vs. NCBI nr
Match: XP_022143011.1 (putative chloride channel-like protein CLC-g isoform X1 [Momordica charantia])

HSP 1 Score: 1218.0 bits (3150), Expect = 0.0e+00
Identity = 631/718 (87.88%), Postives = 657/718 (91.50%), Query Frame = 0

Query: 29  AAAVSTNGDEESIITPLLPPQKAVANSSSQVAIVGANVCPIESLDYEIFDNEFFMQDWRS 88
           A A S+NGDEESI+T LL PQK +ANSSSQVAIVGANVCPIESLDYEIFDNEFFMQDWRS
Sbjct: 2   APAGSSNGDEESIVTSLLAPQKPLANSSSQVAIVGANVCPIESLDYEIFDNEFFMQDWRS 61

Query: 89  REDFQIFQYLIMKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRYATAFL 148
           R DFQIFQYL++KWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRY TAFL
Sbjct: 62  RGDFQIFQYLVVKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRYWTAFL 121

Query: 149 VFSVSNLVLTLFASVITASICPQAAGSGIPEVKAYLNGVDAPGILLPRTVLVKIIGSISI 208
           VFS SNLVLTLFASVITA ICPQAAGSGIPEVKAYLNGVDAPGIL PRT+LVKIIGSISI
Sbjct: 122 VFSFSNLVLTLFASVITAFICPQAAGSGIPEVKAYLNGVDAPGILSPRTLLVKIIGSISI 181

Query: 209 VSSSLIVGKAGPMVHTGACVASLVGQGGFKIFGLTWRSLCHLKNDRDRRDLVTCGAAAGI 268
           VSSSLIVGKAGPMVHTGACVASLVGQGGFK+FGLTWR L HLKNDRDRRDLVTCGAAAGI
Sbjct: 182 VSSSLIVGKAGPMVHTGACVASLVGQGGFKVFGLTWRWLHHLKNDRDRRDLVTCGAAAGI 241

Query: 269 AAAFRAPVGGVLFALEEMASWWRSALLWRSFFTTAVVAVVLRALIDVCLNGLCGLFGKGG 328
           AAAFRAPVGGVLFA EEMASWWRSALLWRSFFTTA+VAV+LR LID CLNGLCGLFGKGG
Sbjct: 242 AAAFRAPVGGVLFAFEEMASWWRSALLWRSFFTTAIVAVILRTLIDFCLNGLCGLFGKGG 301

Query: 329 LIIFDTYSDSPTYHLEDLPPVLALAIVGGILGS-----------------ERH-VYKIFL 388
           LIIFDTYSD P YHL DLPPVLALA +GGILGS                 ER  VYKI L
Sbjct: 302 LIIFDTYSDFPAYHLGDLPPVLALAFLGGILGSLYNFLLNKVLRVYNLIHERGIVYKILL 361

Query: 389 ACSVSIFTSCLLFGLPWFAPCQPCPSSSRETCPTIGRSGNFKKFRCAPGHYNDLASLIFN 448
           ACSVSIFTSCLLFGLPW A CQPCPSS+RE CPTIGRSGNFKKF+C+P HYNDLASLIFN
Sbjct: 362 ACSVSIFTSCLLFGLPWSASCQPCPSSAREICPTIGRSGNFKKFQCSPSHYNDLASLIFN 421

Query: 449 TNDDAIKNLFSKGTDSEFQFSSMLTFFITCFSLSILSYGTVVPAGLFVPVIVTGASYGRF 508
           TNDDAIKNLFSK TDSEFQFSSMLTFFITCFSLSILSYG V PAGLFVPVIVTGASYGRF
Sbjct: 422 TNDDAIKNLFSKDTDSEFQFSSMLTFFITCFSLSILSYGIVAPAGLFVPVIVTGASYGRF 481

Query: 509 VGMVVGPYTNLNHGFFAILGAASFLGGSMRTTVSLCVILLELTNNLLLLPLIMLVLLVSK 568
           VGMVVG YTNL+HGFFAILGAASFLGGSMRTTVSLCVI+LELTNNLLLLPL+MLVLLVSK
Sbjct: 482 VGMVVGRYTNLSHGFFAILGAASFLGGSMRTTVSLCVIMLELTNNLLLLPLVMLVLLVSK 541

Query: 569 TVADAFNGNIYNLIMKAKGFPYLEGHVEPYMRQLTVADVLTSPLQLLNGIEKVRKVVNVL 628
           TVADAFN NIYN IMKAKGFPYLEGHVEPYMRQLTVADVLTSPLQLL+GIEKVR VV+VL
Sbjct: 542 TVADAFNSNIYNSIMKAKGFPYLEGHVEPYMRQLTVADVLTSPLQLLHGIEKVRNVVSVL 601

Query: 629 KWTNHHGFPVIDEPPFSEFPVLYGLILRAHLLMLLKKKAFLSIPAPAPAPEGEDAFKLFS 688
           KWT+HHGFPVIDEPPFSEFP+LYGLILRAHL+MLLKKKAF S  APAPA E +DAFKLFS
Sbjct: 602 KWTSHHGFPVIDEPPFSEFPILYGLILRAHLIMLLKKKAFFS--APAPAQERDDAFKLFS 661

Query: 689 ADDFVKMGSGDIDRIEDVQLTDEEMDMYIDLHPFANTSPCTVLETTSLAKALEVFRET 729
            DDF KM SGD+ RIEDVQLTDEEM+M+IDLHPF NTSPCTVLET SLAKALE+FRET
Sbjct: 662 VDDFAKMDSGDVARIEDVQLTDEEMEMFIDLHPFTNTSPCTVLETMSLAKALEIFRET 717

BLAST of Sgr030558 vs. NCBI nr
Match: XP_038890173.1 (putative chloride channel-like protein CLC-g isoform X1 [Benincasa hispida])

HSP 1 Score: 1207.2 bits (3122), Expect = 0.0e+00
Identity = 621/718 (86.49%), Postives = 656/718 (91.36%), Query Frame = 0

Query: 29  AAAVSTNGDEESIITPLLPPQKAVANSSSQVAIVGANVCPIESLDYEIFDNEFFMQDWRS 88
           A + STNGDEESIITPLL  QK+ ANSSSQVA+VGA++CPIESLDYEIFDNE FMQDWRS
Sbjct: 2   ALSDSTNGDEESIITPLL-AQKSFANSSSQVALVGADICPIESLDYEIFDNELFMQDWRS 61

Query: 89  REDFQIFQYLIMKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRYATAFL 148
           REDFQIFQYL+MKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRY  AFL
Sbjct: 62  REDFQIFQYLVMKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRYGMAFL 121

Query: 149 VFSVSNLVLTLFASVITASICPQAAGSGIPEVKAYLNGVDAPGILLPRTVLVKIIGSISI 208
           VFSVSNLVLTLFASVITA ICPQAAGSGIPEVKAYLNGVDAPGIL PRT+LVKI+GSISI
Sbjct: 122 VFSVSNLVLTLFASVITALICPQAAGSGIPEVKAYLNGVDAPGILSPRTLLVKIVGSISI 181

Query: 209 VSSSLIVGKAGPMVHTGACVASLVGQGGFKIFGLTWRSLCHLKNDRDRRDLVTCGAAAGI 268
           VSSS+IVGKAGPMVHTGACVASLVGQGGFKIFGLTWR   HLKNDRDRRDLVTCGAAAGI
Sbjct: 182 VSSSMIVGKAGPMVHTGACVASLVGQGGFKIFGLTWRWRYHLKNDRDRRDLVTCGAAAGI 241

Query: 269 AAAFRAPVGGVLFALEEMASWWRSALLWRSFFTTAVVAVVLRALIDVCLNGLCGLFGKGG 328
           AAAFRAPVGGVLFA EEMASWWRSALLWRSFFTTA+VAVVLR+LID+CLNGLCGLFGKGG
Sbjct: 242 AAAFRAPVGGVLFAFEEMASWWRSALLWRSFFTTAIVAVVLRSLIDICLNGLCGLFGKGG 301

Query: 329 LIIFDTYSDSPTYHLEDLPPVLALAIVGGILGS------------------ERHVYKIFL 388
           LIIFDTYSD PTYHLEDLPPV  LA +GGILGS                  +  +YKI L
Sbjct: 302 LIIFDTYSDFPTYHLEDLPPVFILAFIGGILGSLYNYLLNKFLRIYHLIHEKGVIYKILL 361

Query: 389 ACSVSIFTSCLLFGLPWFAPCQPCPSSSRETCPTIGRSGNFKKFRCAPGHYNDLASLIFN 448
           ACSVSIFTS LLFGLPWFA CQPCPSS++E CPTIGRSGNFKKF+CAPGHYNDLASLIFN
Sbjct: 362 ACSVSIFTSFLLFGLPWFALCQPCPSSAQEICPTIGRSGNFKKFQCAPGHYNDLASLIFN 421

Query: 449 TNDDAIKNLFSKGTDSEFQFSSMLTFFITCFSLSILSYGTVVPAGLFVPVIVTGASYGRF 508
           TNDDAIKNLFSK TDSEFQ  SMLTFFITCFSLSILSYGTV P GLFVPVIVTGASYGRF
Sbjct: 422 TNDDAIKNLFSKNTDSEFQLLSMLTFFITCFSLSILSYGTVAPVGLFVPVIVTGASYGRF 481

Query: 509 VGMVVGPYTNLNHGFFAILGAASFLGGSMRTTVSLCVILLELTNNLLLLPLIMLVLLVSK 568
           VGMVVGPYTNL+HGFFAILGAASFLGGSMRTTVSLCVI+LELTNNLLLLPLIMLVLL+SK
Sbjct: 482 VGMVVGPYTNLSHGFFAILGAASFLGGSMRTTVSLCVIMLELTNNLLLLPLIMLVLLISK 541

Query: 569 TVADAFNGNIYNLIMKAKGFPYLEGHVEPYMRQLTVADVLTSPLQLLNGIEKVRKVVNVL 628
           TVADAFN NIYNLIMKAKGFPYLEGHVEPYMRQLTVADVLTSPLQL +GIEKVR +VN+L
Sbjct: 542 TVADAFNSNIYNLIMKAKGFPYLEGHVEPYMRQLTVADVLTSPLQLFHGIEKVRNIVNIL 601

Query: 629 KWTNHHGFPVIDEPPFSEFPVLYGLILRAHLLMLLKKKAFLSIPAPAPAPEGEDAFKLFS 688
           + T+HHGFPVIDEPP SEFPVLYGLILRAHL+MLLKKKAFLS+  P    E ED  KLFS
Sbjct: 602 RMTSHHGFPVIDEPPLSEFPVLYGLILRAHLIMLLKKKAFLSV--PILGSESEDPCKLFS 661

Query: 689 ADDFVKMGSGDIDRIEDVQLTDEEMDMYIDLHPFANTSPCTVLETTSLAKALEVFRET 729
           ADDFVKMGSGD++RIED+QLTDEEM+M+IDLHPFANTSPCTVLET S+AKALE+FRET
Sbjct: 662 ADDFVKMGSGDVERIEDIQLTDEEMEMFIDLHPFANTSPCTVLETMSVAKALEIFRET 716

BLAST of Sgr030558 vs. NCBI nr
Match: XP_023536768.1 (putative chloride channel-like protein CLC-g [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1202.6 bits (3110), Expect = 0.0e+00
Identity = 612/717 (85.36%), Postives = 654/717 (91.21%), Query Frame = 0

Query: 30  AAVSTNGDEESIITPLLPPQKAVANSSSQVAIVGANVCPIESLDYEIFDNEFFMQDWRSR 89
           AA  +NGDEESIITPLL PQ+ + NSSSQVA+VGAN+CPIESLDYEIFDN+FFMQDWRSR
Sbjct: 3   AANPSNGDEESIITPLLAPQRLLVNSSSQVALVGANICPIESLDYEIFDNDFFMQDWRSR 62

Query: 90  EDFQIFQYLIMKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRYATAFLV 149
           EDFQIFQYL++KWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRY  AFLV
Sbjct: 63  EDFQIFQYLVIKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRYWMAFLV 122

Query: 150 FSVSNLVLTLFASVITASICPQAAGSGIPEVKAYLNGVDAPGILLPRTVLVKIIGSISIV 209
           FSVSNL+LTLFASVITA ICP AAGSGIPEVKAYLNGVDAPGIL PRT+LVKIIGSI+IV
Sbjct: 123 FSVSNLLLTLFASVITALICPLAAGSGIPEVKAYLNGVDAPGILSPRTLLVKIIGSIAIV 182

Query: 210 SSSLIVGKAGPMVHTGACVASLVGQGGFKIFGLTWRSLCHLKNDRDRRDLVTCGAAAGIA 269
           SSS++VGKAGPMVHTGACVASLVGQG FK+FGLTWR L HLK DRDRRDLVTCGAAAGIA
Sbjct: 183 SSSMVVGKAGPMVHTGACVASLVGQGCFKVFGLTWRWLYHLKKDRDRRDLVTCGAAAGIA 242

Query: 270 AAFRAPVGGVLFALEEMASWWRSALLWRSFFTTAVVAVVLRALIDVCLNGLCGLFGKGGL 329
           AAFRAPVGGVLFA EEMASWWRSALLWR+FFTTA+VAVVLR+LIDVCLNGLCGLFGKGGL
Sbjct: 243 AAFRAPVGGVLFAFEEMASWWRSALLWRAFFTTAIVAVVLRSLIDVCLNGLCGLFGKGGL 302

Query: 330 IIFDTYSDSPTYHLEDLPPVLALAIVGGILGS------------------ERHVYKIFLA 389
           IIFDTYSD P+YHL+DLPPVLALA +GGILGS                  +  VYK+ LA
Sbjct: 303 IIFDTYSDFPSYHLKDLPPVLALAFIGGILGSFYNFLLTKVLRVYNLIHEKGIVYKVLLA 362

Query: 390 CSVSIFTSCLLFGLPWFAPCQPCPSSSRETCPTIGRSGNFKKFRCAPGHYNDLASLIFNT 449
           CSVSIFTSCLLFGLPWFA CQPCPSS+RE CPTIGRSGNFKKF+C+ GHYNDLASLIFNT
Sbjct: 363 CSVSIFTSCLLFGLPWFASCQPCPSSAREICPTIGRSGNFKKFQCSSGHYNDLASLIFNT 422

Query: 450 NDDAIKNLFSKGTDSEFQFSSMLTFFITCFSLSILSYGTVVPAGLFVPVIVTGASYGRFV 509
           NDDAIKNLFSKGTDSEFQFSSMLTFF+TCFSLS+LSYGTV P GLFVPVIVTGASYGRFV
Sbjct: 423 NDDAIKNLFSKGTDSEFQFSSMLTFFVTCFSLSVLSYGTVAPVGLFVPVIVTGASYGRFV 482

Query: 510 GMVVGPYTNLNHGFFAILGAASFLGGSMRTTVSLCVILLELTNNLLLLPLIMLVLLVSKT 569
           GMVVGPYTNL+HGFFAILGAASFLGGSMRTTVSLCVI+LELTNNLLLLPL+MLVLL+SKT
Sbjct: 483 GMVVGPYTNLSHGFFAILGAASFLGGSMRTTVSLCVIMLELTNNLLLLPLVMLVLLISKT 542

Query: 570 VADAFNGNIYNLIMKAKGFPYLEGHVEPYMRQLTVADVLTSPLQLLNGIEKVRKVVNVLK 629
           VADAFN NIYN IMKAKGFPYLEGHVEPYMRQLTVA VLTSPLQL  GIEKVR VVNVLK
Sbjct: 543 VADAFNCNIYNQIMKAKGFPYLEGHVEPYMRQLTVASVLTSPLQLFRGIEKVRNVVNVLK 602

Query: 630 WTNHHGFPVIDEPPFSEFPVLYGLILRAHLLMLLKKKAFLSIPAPAPAPEGEDAFKLFSA 689
           WT+HHGFP+IDEPPFSEFPVLYGLILRAHL++LLKKKAFLS+  P    E +DA KL SA
Sbjct: 603 WTSHHGFPIIDEPPFSEFPVLYGLILRAHLIVLLKKKAFLSV--PTLGLERQDALKLLSA 662

Query: 690 DDFVKMGSGDIDRIEDVQLTDEEMDMYIDLHPFANTSPCTVLETTSLAKALEVFRET 729
           DDF  MGSGD+DRIED+QLTDEEM+M+IDLHPFANTSPCTVLET SLAKA  +FRET
Sbjct: 663 DDFAVMGSGDVDRIEDIQLTDEEMEMFIDLHPFANTSPCTVLETMSLAKAFAIFRET 717

BLAST of Sgr030558 vs. NCBI nr
Match: KAG6586372.1 (putative chloride channel-like protein CLC-g, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1201.8 bits (3108), Expect = 0.0e+00
Identity = 612/717 (85.36%), Postives = 654/717 (91.21%), Query Frame = 0

Query: 30  AAVSTNGDEESIITPLLPPQKAVANSSSQVAIVGANVCPIESLDYEIFDNEFFMQDWRSR 89
           AA  +NGDEESIITPLL PQK++ NSSSQVA+VGAN+CPIESLDYEIFDN+FFMQDWRSR
Sbjct: 3   AANPSNGDEESIITPLLAPQKSLVNSSSQVALVGANICPIESLDYEIFDNDFFMQDWRSR 62

Query: 90  EDFQIFQYLIMKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRYATAFLV 149
           EDFQIFQYL++KWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRY  AFLV
Sbjct: 63  EDFQIFQYLVIKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRYWMAFLV 122

Query: 150 FSVSNLVLTLFASVITASICPQAAGSGIPEVKAYLNGVDAPGILLPRTVLVKIIGSISIV 209
           FSVSNL+LTLFASVITA ICP AAGSGIPEVKAYLNGVDAPGIL PRT+LVKIIGSI+IV
Sbjct: 123 FSVSNLLLTLFASVITALICPLAAGSGIPEVKAYLNGVDAPGILSPRTLLVKIIGSIAIV 182

Query: 210 SSSLIVGKAGPMVHTGACVASLVGQGGFKIFGLTWRSLCHLKNDRDRRDLVTCGAAAGIA 269
           SSS++VGKAGPMVHTGACVASLVGQG FK+FGLTWR L HLK DRDRRDLVTCGAAAGIA
Sbjct: 183 SSSMVVGKAGPMVHTGACVASLVGQGCFKVFGLTWRWLYHLKKDRDRRDLVTCGAAAGIA 242

Query: 270 AAFRAPVGGVLFALEEMASWWRSALLWRSFFTTAVVAVVLRALIDVCLNGLCGLFGKGGL 329
           AAFRAPVGGVLFA EEMASWWRSALLWR+FFTTA+VAVVLR+LIDVCLNGLCGLFGKGGL
Sbjct: 243 AAFRAPVGGVLFAFEEMASWWRSALLWRAFFTTAIVAVVLRSLIDVCLNGLCGLFGKGGL 302

Query: 330 IIFDTYSDSPTYHLEDLPPVLALAIVGGILGS------------------ERHVYKIFLA 389
           IIFDTYSD P+YHL+DLPPVLALA +GGILGS                  +  VYK+ LA
Sbjct: 303 IIFDTYSDFPSYHLKDLPPVLALAFIGGILGSFYNFLLTKVLRVYNLIHEKGIVYKVLLA 362

Query: 390 CSVSIFTSCLLFGLPWFAPCQPCPSSSRETCPTIGRSGNFKKFRCAPGHYNDLASLIFNT 449
           CSVSIFTSCLLFGLPWFA CQPCPSS+RE CPTIGRSGNFKKF+C+ GHYNDLASLIFNT
Sbjct: 363 CSVSIFTSCLLFGLPWFASCQPCPSSAREICPTIGRSGNFKKFQCSSGHYNDLASLIFNT 422

Query: 450 NDDAIKNLFSKGTDSEFQFSSMLTFFITCFSLSILSYGTVVPAGLFVPVIVTGASYGRFV 509
           NDDAIKNLFSKGTDSEFQFSSMLTFF+TCFSLS+LSYGTV P GLFVPVIVTGASYGRFV
Sbjct: 423 NDDAIKNLFSKGTDSEFQFSSMLTFFVTCFSLSVLSYGTVAPVGLFVPVIVTGASYGRFV 482

Query: 510 GMVVGPYTNLNHGFFAILGAASFLGGSMRTTVSLCVILLELTNNLLLLPLIMLVLLVSKT 569
           GMVVGPYTNL+HGFFAILGAASFLGGSMRTTVSLCVI+LELTNNLLLLPL+MLVLL+SKT
Sbjct: 483 GMVVGPYTNLSHGFFAILGAASFLGGSMRTTVSLCVIMLELTNNLLLLPLVMLVLLISKT 542

Query: 570 VADAFNGNIYNLIMKAKGFPYLEGHVEPYMRQLTVADVLTSPLQLLNGIEKVRKVVNVLK 629
           VADAFN NIYN IMKAKGFPYLEGHVEPYMRQLTVA VLTSPLQL  GIEKVR VVNVLK
Sbjct: 543 VADAFNCNIYNQIMKAKGFPYLEGHVEPYMRQLTVASVLTSPLQLFRGIEKVRNVVNVLK 602

Query: 630 WTNHHGFPVIDEPPFSEFPVLYGLILRAHLLMLLKKKAFLSIPAPAPAPEGEDAFKLFSA 689
           WT+HHGFP+IDEPPFSEFPVLYGLILRAHL++LLKKKAFLS+  P    E +DA KL SA
Sbjct: 603 WTSHHGFPIIDEPPFSEFPVLYGLILRAHLIVLLKKKAFLSV--PTLGLERQDALKLLSA 662

Query: 690 DDFVKMGSGDIDRIEDVQLTDEEMDMYIDLHPFANTSPCTVLETTSLAKALEVFRET 729
           DDF  MGSGD+DRIED+QLTDEEM+M+IDLH FANTSPCTVLET SLAKA  +FRET
Sbjct: 663 DDFAMMGSGDVDRIEDIQLTDEEMEMFIDLHSFANTSPCTVLETMSLAKAFAIFRET 717

BLAST of Sgr030558 vs. ExPASy Swiss-Prot
Match: P60300 (Putative chloride channel-like protein CLC-g OS=Arabidopsis thaliana OX=3702 GN=CLC-G PE=1 SV=2)

HSP 1 Score: 977.2 bits (2525), Expect = 1.1e-283
Identity = 497/711 (69.90%), Postives = 582/711 (81.86%), Query Frame = 0

Query: 38  EESIITPLLPPQKAVANSSSQVAIVGANVCPIESLDYEIFDNEFFMQDWRSREDFQIFQY 97
           E+S+  PLLP  +   NS+SQVAIVGANVCPIESLDYEI +N+FF QDWR R   +IFQY
Sbjct: 7   EDSVAVPLLPSLRRATNSTSQVAIVGANVCPIESLDYEIAENDFFKQDWRGRSKVEIFQY 66

Query: 98  LIMKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRYATAFLVFSVSNLVL 157
           + MKWL CF IG+I+ L+GF NNLAVEN+AG KFVVTSNMM+ GR+A  F+VFSV+NL+L
Sbjct: 67  VFMKWLLCFCIGIIVSLIGFANNLAVENLAGVKFVVTSNMMIAGRFAMGFVVFSVTNLIL 126

Query: 158 TLFASVITASICPQAAGSGIPEVKAYLNGVDAPGILLPRTVLVKIIGSISIVSSSLIVGK 217
           TLFASVITA + P AAGSGIPEVKAYLNGVDAP I   RT+++KIIG+IS VS+SL++GK
Sbjct: 127 TLFASVITAFVAPAAAGSGIPEVKAYLNGVDAPEIFSLRTLIIKIIGNISAVSASLLIGK 186

Query: 218 AGPMVHTGACVASLVGQGGFKIFGLTWRSLCHLKNDRDRRDLVTCGAAAGIAAAFRAPVG 277
           AGPMVHTGACVAS++GQGG K + LTWR L   KNDRDRRDLVTCGAAAGIAA+FRAPVG
Sbjct: 187 AGPMVHTGACVASILGQGGSKRYRLTWRWLRFFKNDRDRRDLVTCGAAAGIAASFRAPVG 246

Query: 278 GVLFALEEMASWWRSALLWRSFFTTAVVAVVLRALIDVCLNGLCGLFGKGGLIIFDTYSD 337
           GVLFALEEM+SWWRSALLWR FF+TAVVA+VLRALIDVCL+G CGLFGKGGLI+FD YS+
Sbjct: 247 GVLFALEEMSSWWRSALLWRIFFSTAVVAIVLRALIDVCLSGKCGLFGKGGLIMFDVYSE 306

Query: 338 SPTYHLEDLPPVLALAIVGGILGS-----------------ERHV-YKIFLACSVSIFTS 397
           + +YHL D+ PVL L +VGGILGS                 E+ V +KI LAC++SIFTS
Sbjct: 307 NASYHLGDVLPVLLLGVVGGILGSLYNFLLDKVLRAYNYIYEKGVTWKILLACAISIFTS 366

Query: 398 CLLFGLPWFAPCQPCPSSSRETCPTIGRSGNFKKFRCAPGHYNDLASLIFNTNDDAIKNL 457
           CLLFGLP+ A CQPCP  + E CPTIGRSGNFKK++C PGHYNDLASLIFNTNDDAIKNL
Sbjct: 367 CLLFGLPFLASCQPCPVDALEECPTIGRSGNFKKYQCPPGHYNDLASLIFNTNDDAIKNL 426

Query: 458 FSKGTDSEFQFSSMLTFFITCFSLSILSYGTVVPAGLFVPVIVTGASYGRFVGMVVGPYT 517
           FSK TD EF + S+L FF+TCF LSI SYG V PAGLFVPVIVTGASYGRFVGM++G  +
Sbjct: 427 FSKNTDFEFHYFSVLVFFVTCFFLSIFSYGIVAPAGLFVPVIVTGASYGRFVGMLLGSNS 486

Query: 518 NLNHGFFAILGAASFLGGSMRTTVSLCVILLELTNNLLLLPLIMLVLLVSKTVADAFNGN 577
           NLNHG FA+LGAASFLGG+MR TVS CVILLELTNNLLLLP++M+VLL+SKTVAD FN N
Sbjct: 487 NLNHGLFAVLGAASFLGGTMRMTVSTCVILLELTNNLLLLPMMMVVLLISKTVADGFNAN 546

Query: 578 IYNLIMKAKGFPYLEGHVEPYMRQLTVADVLTSPLQLLNGIEKVRKVVNVLKWTNHHGFP 637
           IYNLIMK KGFPYL  H EPYMRQL V DV+T PLQ+ NGIEKV  +V+VLK TNH+GFP
Sbjct: 547 IYNLIMKLKGFPYLYSHAEPYMRQLLVGDVVTGPLQVFNGIEKVETIVHVLKTTNHNGFP 606

Query: 638 VIDEPPFSEFPVLYGLILRAHLLMLLKKKAFLSIPAPAPAPEGEDAFKLFSADDFVKMGS 697
           V+D PP +  PVL+GLILRAH+L LLKK+ F+    P+P     +    F A++F K GS
Sbjct: 607 VVDGPPLAAAPVLHGLILRAHILTLLKKRVFM----PSPVACDSNTLSQFKAEEFAKKGS 666

Query: 698 GDIDRIEDVQLTDEEMDMYIDLHPFANTSPCTVLETTSLAKALEVFRETDV 731
           G  D+IEDV+L++EE++MY+DLHPF+N SP TV+ET SLAKAL +FRE  +
Sbjct: 667 GRSDKIEDVELSEEELNMYLDLHPFSNASPYTVVETMSLAKALILFREVGI 713

BLAST of Sgr030558 vs. ExPASy Swiss-Prot
Match: Q96282 (Chloride channel protein CLC-c OS=Arabidopsis thaliana OX=3702 GN=CLC-C PE=1 SV=1)

HSP 1 Score: 770.8 bits (1989), Expect = 1.5e-221
Identity = 398/693 (57.43%), Postives = 499/693 (72.01%), Query Frame = 0

Query: 54  NSSSQVAIVGANVCPIESLDYEIFDNEFFMQDWRSREDFQIFQYLIMKWLSCFLIGLIMG 113
           N++SQ+AIVGAN CPIESLDYEIF+N+FF QDWRSR+  +I QY  +KW   FLIGL  G
Sbjct: 48  NTTSQIAIVGANTCPIESLDYEIFENDFFKQDWRSRKKIEILQYTFLKWALAFLIGLATG 107

Query: 114 LVGFFNNLAVENIAGKKFVVTSNMMLEGRYATAFLVFSVSNLVLTLFASVITASICPQAA 173
           LVGF NNL VENIAG K ++  N+ML+ +Y  AF  F+  NL+L   A+ + A I P AA
Sbjct: 108 LVGFLNNLGVENIAGFKLLLIGNLMLKEKYFQAFFAFAGCNLILATAAASLCAFIAPAAA 167

Query: 174 GSGIPEVKAYLNGVDAPGILLPRTVLVKIIGSISIVSSSLIVGKAGPMVHTGACVASLVG 233
           GSGIPEVKAYLNG+DA  IL P T+ VKI GSI  V++  +VGK GPMVHTGAC+A+L+G
Sbjct: 168 GSGIPEVKAYLNGIDAYSILAPSTLFVKIFGSIFGVAAGFVVGKEGPMVHTGACIANLLG 227

Query: 234 QGGFKIFGLTWRSLCHLKNDRDRRDLVTCGAAAGIAAAFRAPVGGVLFALEEMASWWRSA 293
           QGG K + LTW+ L   KNDRDRRDL+TCGAAAG+AAAFRAPVGGVLFALEE ASWWR+A
Sbjct: 228 QGGSKKYRLTWKWLRFFKNDRDRRDLITCGAAAGVAAAFRAPVGGVLFALEEAASWWRNA 287

Query: 294 LLWRSFFTTAVVAVVLRALIDVCLNGLCGLFGKGGLIIFDTYSDSPTYHLEDLPPVLALA 353
           LLWR+FFTTAVVAVVLR+LI+ C +G CGLFGKGGLI+FD  S    Y   DL  ++ L 
Sbjct: 288 LLWRTFFTTAVVAVVLRSLIEFCRSGRCGLFGKGGLIMFDVNSGPVLYSTPDLLAIVFLG 347

Query: 354 IVGGILGSERHV------------------YKIFLACSVSIFTSCLLFGLPWFAPCQPCP 413
           ++GG+LGS  +                   +KI L  +VSI +SC  FGLPW + C PCP
Sbjct: 348 VIGGVLGSLYNYLVDKVLRTYSIINEKGPRFKIMLVMAVSILSSCCAFGLPWLSQCTPCP 407

Query: 414 SSSRE-TCPTIGRSGNFKKFRCAPGHYNDLASLIFNTNDDAIKNLFSKGTDSEFQFSSML 473
               E  CP++GRS  +K F+C P HYNDL+SL+ NTNDDAI+NLF+  +++EF  S++ 
Sbjct: 408 IGIEEGKCPSVGRSSIYKSFQCPPNHYNDLSSLLLNTNDDAIRNLFTSRSENEFHISTLA 467

Query: 474 TFFITCFSLSILSYGTVVPAGLFVPVIVTGASYGRFVGMVVGPYTNLNHGFFAILGAASF 533
            FF+  + L I++YG  +P+GLF+PVI+ GASYGR VG ++GP + L+ G F++LGAASF
Sbjct: 468 IFFVAVYCLGIITYGIAIPSGLFIPVILAGASYGRLVGRLLGPVSQLDVGLFSLLGAASF 527

Query: 534 LGGSMRTTVSLCVILLELTNNLLLLPLIMLVLLVSKTVADAFNGNIYNLIMKAKGFPYLE 593
           LGG+MR TVSLCVILLELTNNLL+LPL+MLVLL+SKTVAD FN  +Y+ I+  KG PY+E
Sbjct: 528 LGGTMRMTVSLCVILLELTNNLLMLPLVMLVLLISKTVADCFNRGVYDQIVTMKGLPYME 587

Query: 594 GHVEPYMRQLTVADVLTSPLQLLNGIEKVRKVVNVLKWTNHHGFPVIDEPPFSEFPVLYG 653
            H EPYMR L   DV++  L   + +EKV  +   LK T H+GFPVIDEPPF+E   L G
Sbjct: 588 DHAEPYMRNLVAKDVVSGALISFSRVEKVGVIWQALKMTRHNGFPVIDEPPFTEASELCG 647

Query: 654 LILRAHLLMLLKKKAFLSIPAPAPAPEGEDAFKLFSADDFVKMGSGDIDRIEDVQLTDEE 713
           + LR+HLL+LL+ K F    +      G    +   A DF K G G   +IED+ L++EE
Sbjct: 648 IALRSHLLVLLQGKKF----SKQRTTFGSQILRSCKARDFGKAGLGKGLKIEDLDLSEEE 707

Query: 714 MDMYIDLHPFANTSPCTVLETTSLAKALEVFRE 728
           M+MY+DLHP  NTSP TVLET SLAKA  +FR+
Sbjct: 708 MEMYVDLHPITNTSPYTVLETLSLAKAAILFRQ 736

BLAST of Sgr030558 vs. ExPASy Swiss-Prot
Match: P92942 (Chloride channel protein CLC-b OS=Arabidopsis thaliana OX=3702 GN=CLC-B PE=1 SV=1)

HSP 1 Score: 679.9 bits (1753), Expect = 3.5e-194
Identity = 359/720 (49.86%), Postives = 489/720 (67.92%), Query Frame = 0

Query: 36  GDEES--IITPLLPPQKAVANSSSQVAIVGANVCPIESLDYEIFDNEFFMQDWRSREDFQ 95
           GD ES  +  PL+   + +  SS+ +A+VGA V  IESLDYEI +N+ F  DWR R   Q
Sbjct: 20  GDPESNTLNQPLVKANRTL--SSTPLALVGAKVSHIESLDYEINENDLFKHDWRKRSKAQ 79

Query: 96  IFQYLIMKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRYATAFLVFSVS 155
           + QY+ +KW    L+GL  GL+    NLAVENIAG K +   + + + RY T  +V   +
Sbjct: 80  VLQYVFLKWTLACLVGLFTGLIATLINLAVENIAGYKLLAVGHFLTQERYVTGLMVLVGA 139

Query: 156 NLVLTLFASVITASICPQAAGSGIPEVKAYLNGVDAPGILLPRTVLVKIIGSISIVSSSL 215
           NL LTL ASV+     P AAG GIPE+KAYLNGVD P +    T++VKI+GSI  V++ L
Sbjct: 140 NLGLTLVASVLCVCFAPTAAGPGIPEIKAYLNGVDTPNMFGATTMIVKIVGSIGAVAAGL 199

Query: 216 IVGKAGPMVHTGACVASLVGQGGFKIFGLTWRSLCHLKNDRDRRDLVTCGAAAGIAAAFR 275
            +GK GP+VH G+C+ASL+GQGG     + WR L +  NDRDRRDL+TCG+AAG+ AAFR
Sbjct: 200 DLGKEGPLVHIGSCIASLLGQGGTDNHRIKWRWLRYFNNDRDRRDLITCGSAAGVCAAFR 259

Query: 276 APVGGVLFALEEMASWWRSALLWRSFFTTAVVAVVLRALIDVCLNGLCGLFGKGGLIIFD 335
           +PVGGVLFALEE+A+WWRSALLWR+FF+TAVV VVLR  I++C +G CGLFGKGGLI+FD
Sbjct: 260 SPVGGVLFALEEVATWWRSALLWRTFFSTAVVVVVLREFIEICNSGKCGLFGKGGLIMFD 319

Query: 336 TYSDSPTYHLEDLPPVLALAIVGGILGS------------------ERHVYKIFLACSVS 395
               + TYH+ D+ PV+ + ++GGILGS                  +  ++K+ L+ +VS
Sbjct: 320 VSHVTYTYHVTDIIPVMLIGVIGGILGSLYNHLLHKVLRLYNLINEKGKIHKVLLSLTVS 379

Query: 396 IFTSCLLFGLPWFAPCQPCPSSSRETCPTIGRSGNFKKFRCAPGHYNDLASLIFNTNDDA 455
           +FTS  L+GLP+ A C+PC  S  E CPT GRSGNFK+F C  G+YNDLA+L+  TNDDA
Sbjct: 380 LFTSVCLYGLPFLAKCKPCDPSIDEICPTNGRSGNFKQFHCPKGYYNDLATLLLTTNDDA 439

Query: 456 IKNLFSKGTDSEFQFSSMLTFFITCFSLSILSYGTVVPAGLFVPVIVTGASYGRFVGMVV 515
           ++NLFS  T +EF   S+  FF+    L + ++G   P+GLF+P+I+ GA+YGR +G  +
Sbjct: 440 VRNLFSSNTPNEFGMGSLWIFFVLYCILGLFTFGIATPSGLFLPIILMGAAYGRMLGAAM 499

Query: 516 GPYTNLNHGFFAILGAASFLGGSMRTTVSLCVILLELTNNLLLLPLIMLVLLVSKTVADA 575
           G YT+++ G +A+LGAA+ + GSMR TVSLCVI LELTNNLLLLP+ M+VLL++KTV D+
Sbjct: 500 GSYTSIDQGLYAVLGAAALMAGSMRMTVSLCVIFLELTNNLLLLPITMIVLLIAKTVGDS 559

Query: 576 FNGNIYNLIMKAKGFPYLEGHVEPYMRQLTVADV--LTSPLQLLNGIEKVRKVVNVLKWT 635
           FN +IY++I+  KG P+LE + EP+MR LTV ++     P+  L G+EKV  +V+VLK T
Sbjct: 560 FNPSIYDIILHLKGLPFLEANPEPWMRNLTVGELGDAKPPVVTLQGVEKVSNIVDVLKNT 619

Query: 636 NHHGFPVIDEPPFSEFPV------LYGLILRAHLLMLLKKKAFLSIPAPAPAPEGEDAFK 695
            H+ FPV+DE    +  +      L+GLILRAHL+ +LKK+ FL+        E  +  +
Sbjct: 620 THNAFPVLDEAEVPQVGLATGATELHGLILRAHLVKVLKKRWFLT---EKRRTEEWEVRE 679

Query: 696 LFSADDFVKMGSGDIDRIEDVQLTDEEMDMYIDLHPFANTSPCTVLETTSLAKALEVFRE 728
            F  D+  +      D  +DV +T  EM+MY+DLHP  NT+P TV+E  S+AKAL +FR+
Sbjct: 680 KFPWDELAERE----DNFDDVAITSAEMEMYVDLHPLTNTTPYTVMENMSVAKALVLFRQ 730

BLAST of Sgr030558 vs. ExPASy Swiss-Prot
Match: P92941 (Chloride channel protein CLC-a OS=Arabidopsis thaliana OX=3702 GN=CLC-A PE=1 SV=2)

HSP 1 Score: 667.9 bits (1722), Expect = 1.4e-190
Identity = 355/719 (49.37%), Postives = 484/719 (67.32%), Query Frame = 0

Query: 35  NGDEE-------SIITPLLPPQKAVANSSSQVAIVGANVCPIESLDYEIFDNEFFMQDWR 94
           NG+EE       ++  PLL   + +  SS+ +A+VGA V  IESLDYEI +N+ F  DWR
Sbjct: 15  NGEEEGEDPENNTLNQPLLKRHRTL--SSTPLALVGAKVSHIESLDYEINENDLFKHDWR 74

Query: 95  SREDFQIFQYLIMKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRYATAF 154
           SR   Q+FQY+ +KW    L+GL  GL+    NLAVENIAG K +     + + R+ T  
Sbjct: 75  SRSKAQVFQYIFLKWTLACLVGLFTGLIATLINLAVENIAGYKLLAVGYYIAQDRFWTGL 134

Query: 155 LVFSVSNLVLTLFASVITASICPQAAGSGIPEVKAYLNGVDAPGILLPRTVLVKIIGSIS 214
           +VF+ +NL LTL A+V+     P AAG GIPE+KAYLNG+D P +    T++VKI+GSI 
Sbjct: 135 MVFTGANLGLTLVATVLVVYFAPTAAGPGIPEIKAYLNGIDTPNMFGFTTMMVKIVGSIG 194

Query: 215 IVSSSLIVGKAGPMVHTGACVASLVGQGGFKIFGLTWRSLCHLKNDRDRRDLVTCGAAAG 274
            V++ L +GK GP+VH G+C+ASL+GQGG     + WR L +  NDRDRRDL+TCG+A+G
Sbjct: 195 AVAAGLDLGKEGPLVHIGSCIASLLGQGGPDNHRIKWRWLRYFNNDRDRRDLITCGSASG 254

Query: 275 IAAAFRAPVGGVLFALEEMASWWRSALLWRSFFTTAVVAVVLRALIDVCLNGLCGLFGKG 334
           + AAFR+PVGGVLFALEE+A+WWRSALLWR+FF+TAVV VVLRA I++C +G CGLFG G
Sbjct: 255 VCAAFRSPVGGVLFALEEVATWWRSALLWRTFFSTAVVVVVLRAFIEICNSGKCGLFGSG 314

Query: 335 GLIIFDTYSDSPTYHLEDLPPVLALAIVGGILGS------------------ERHVYKIF 394
           GLI+FD       YH  D+ PV  + + GGILGS                  +  ++K+ 
Sbjct: 315 GLIMFDVSHVEVRYHAADIIPVTLIGVFGGILGSLYNHLLHKVLRLYNLINQKGKIHKVL 374

Query: 395 LACSVSIFTSCLLFGLPWFAPCQPCPSSSRETCPTIGRSGNFKKFRCAPGHYNDLASLIF 454
           L+  VS+FTS  LFGLP+ A C+PC  S  E CPT GRSGNFK+F C  G+YNDL++L+ 
Sbjct: 375 LSLGVSLFTSVCLFGLPFLAECKPCDPSIDEICPTNGRSGNFKQFNCPNGYYNDLSTLLL 434

Query: 455 NTNDDAIKNLFSKGTDSEFQFSSMLTFFITCFSLSILSYGTVVPAGLFVPVIVTGASYGR 514
            TNDDA++N+FS  T +EF   S+  FF     L ++++G   P+GLF+P+I+ G++YGR
Sbjct: 435 TTNDDAVRNIFSSNTPNEFGMVSLWIFFGLYCILGLITFGIATPSGLFLPIILMGSAYGR 494

Query: 515 FVGMVVGPYTNLNHGFFAILGAASFLGGSMRTTVSLCVILLELTNNLLLLPLIMLVLLVS 574
            +G  +G YTN++ G +A+LGAAS + GSMR TVSLCVI LELTNNLLLLP+ M VLL++
Sbjct: 495 MLGTAMGSYTNIDQGLYAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITMFVLLIA 554

Query: 575 KTVADAFNGNIYNLIMKAKGFPYLEGHVEPYMRQLTVADV--LTSPLQLLNGIEKVRKVV 634
           KTV D+FN +IY +I+  KG P+LE + EP+MR LTV ++     P+  LNG+EKV  +V
Sbjct: 555 KTVGDSFNLSIYEIILHLKGLPFLEANPEPWMRNLTVGELNDAKPPVVTLNGVEKVANIV 614

Query: 635 NVLKWTNHHGFPVIDEPPFSEFPVLYGLILRAHLLMLLKKKAFLSIPAPAPAPEGEDAFK 694
           +VL+ T H+ FPV+D    +    L+GLILRAHL+ +LKK+ FL+        E  + F 
Sbjct: 615 DVLRNTTHNAFPVLDGADQNTGTELHGLILRAHLVKVLKKRWFLNEKRRTEEWEVREKFT 674

Query: 695 LFSADDFVKMGSGDIDRIEDVQLTDEEMDMYIDLHPFANTSPCTVLETTSLAKALEVFR 727
                  V++   + D  +DV +T  EM +Y+DLHP  NT+P TV+++ S+AKAL +FR
Sbjct: 675 P------VELAERE-DNFDDVAITSSEMQLYVDLHPLTNTTPYTVVQSMSVAKALVLFR 724

BLAST of Sgr030558 vs. ExPASy Swiss-Prot
Match: P92943 (Chloride channel protein CLC-d OS=Arabidopsis thaliana OX=3702 GN=CLC-D PE=1 SV=2)

HSP 1 Score: 549.7 bits (1415), Expect = 5.4e-155
Identity = 321/688 (46.66%), Postives = 426/688 (61.92%), Query Frame = 0

Query: 69  IESLDYEIFDNEFFMQDWRSREDFQIFQYLIMKWLSCFLIGLIMGLVGFFNNLAVENIAG 128
           + SLDYE+ +N  + ++   R    +  Y+ +KW    LIG+  GL   F NL+VEN AG
Sbjct: 44  VNSLDYEVIENYAYREEQAHRGKLYVGYYVAVKWFFSLLIGIGTGLAAVFINLSVENFAG 103

Query: 129 KKFVVTSNMMLEGRYATAFLVFSVSNLVLTLFASVITASICPQAAGSGIPEVKAYLNGVD 188
            KF +T   +++  Y   F+V+ + NLVL   ++ I     P AAGSGIPE+K YLNG+D
Sbjct: 104 WKFALTF-AIIQKSYFAGFIVYLLINLVLVFSSAYIITQFAPAAAGSGIPEIKGYLNGID 163

Query: 189 APGILLPRTVLVKIIGSISIVSSSLIVGKAGPMVHTGACVASLVGQGGFKIFGLTWRSLC 248
            PG LL RT++ KI GSI  V   L +GK GP+VHTGAC+ASL+GQGG   + L  R   
Sbjct: 164 IPGTLLFRTLIGKIFGSIGSVGGGLALGKEGPLVHTGACIASLLGQGGSTKYHLNSRWPQ 223

Query: 249 HLKNDRDRRDLVTCGAAAGIAAAFRAPVGGVLFALEEMASWWRSALLWRSFFTTAVVAVV 308
             K+DRDRRDLVTCG AAG+AAAFRAPVGGVLFALEE+ SWWRS L+WR FFT+A+VAVV
Sbjct: 224 LFKSDRDRRDLVTCGCAAGVAAAFRAPVGGVLFALEEVTSWWRSQLMWRVFFTSAIVAVV 283

Query: 309 LRALIDVCLNGLCGLFGKGGLIIFDTYSDSPTYHLEDLPPVLALAIVGGILGS------- 368
           +R  +  C +G+CG FG GG II+D       Y+ ++L P+  + ++GG+LG+       
Sbjct: 284 VRTAMGWCKSGICGHFGGGGFIIWDVSDGQDDYYFKELLPMAVIGVIGGLLGALFNQLTL 343

Query: 369 ------------ERHVYKIFLACSVSIFTSCLLFGLPWFAPCQPCPSSSRET---CP-TI 428
                       + +  KI  AC +S  TS + FGLP    C PCP S  ++   CP   
Sbjct: 344 YMTSWRRNSLHKKGNRVKIIEACIISCITSAISFGLPLLRKCSPCPESVPDSGIECPRPP 403

Query: 429 GRSGNFKKFRC-APGHYNDLASLIFNTNDDAIKNLFSKGTDSEFQFSSMLTFFITCFSLS 488
           G  GN+  F C     YNDLA++ FNT DDAI+NLFS  T  EF   S+LTF    ++L+
Sbjct: 404 GMYGNYVNFFCKTDNEYNDLATIFFNTQDDAIRNLFSAKTMREFSAQSLLTFLAMFYTLA 463

Query: 489 ILSYGTVVPAGLFVPVIVTGASYGRFVGMVVGPY---TNLNHGFFAILGAASFLGGSMRT 548
           ++++GT VPAG FVP I+ G++YGR VGM V  +    N+  G +A+LGAASFLGGSMR 
Sbjct: 464 VVTFGTAVPAGQFVPGIMIGSTYGRLVGMFVVRFYKKLNIEEGTYALLGAASFLGGSMRM 523

Query: 549 TVSLCVILLELTNNLLLLPLIMLVLLVSKTVADAFNGNIYNLIMKAKGFPYLEGHVEPYM 608
           TVSLCVI++E+TNNL LLPLIMLVLL+SK V DAFN  +Y +  + KG P LE   + +M
Sbjct: 524 TVSLCVIMVEITNNLKLLPLIMLVLLISKAVGDAFNEGLYEVQARLKGIPLLESRPKYHM 583

Query: 609 RQLTVADVLTSPLQL-LNGIEKVRKVVNVLKWTNHHGFPVIDEPPFSEFPVLYGLILRAH 668
           RQ+   +   S   + L  + +V  V ++L    H+GFPVID     E  V+ GL+LR+H
Sbjct: 584 RQMIAKEACQSQKVISLPRVIRVADVASILGSNKHNGFPVIDHTRSGETLVI-GLVLRSH 643

Query: 669 LLMLLKKKA-FLSIPAPAPAPEGEDAFKLFSADDFVKMGSGDIDRIEDVQLTDEEMDMYI 728
           LL+LL+ K  F   P P   P   +    FS  +F K  S     IED+ LT ++++MYI
Sbjct: 644 LLVLLQSKVDFQHSPLPCD-PSARNIRHSFS--EFAKPVSSKGLCIEDIHLTSDDLEMYI 703

BLAST of Sgr030558 vs. ExPASy TrEMBL
Match: A0A6J1CNX5 (putative chloride channel-like protein CLC-g isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013000 PE=3 SV=1)

HSP 1 Score: 1218.0 bits (3150), Expect = 0.0e+00
Identity = 631/718 (87.88%), Postives = 657/718 (91.50%), Query Frame = 0

Query: 29  AAAVSTNGDEESIITPLLPPQKAVANSSSQVAIVGANVCPIESLDYEIFDNEFFMQDWRS 88
           A A S+NGDEESI+T LL PQK +ANSSSQVAIVGANVCPIESLDYEIFDNEFFMQDWRS
Sbjct: 2   APAGSSNGDEESIVTSLLAPQKPLANSSSQVAIVGANVCPIESLDYEIFDNEFFMQDWRS 61

Query: 89  REDFQIFQYLIMKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRYATAFL 148
           R DFQIFQYL++KWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRY TAFL
Sbjct: 62  RGDFQIFQYLVVKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRYWTAFL 121

Query: 149 VFSVSNLVLTLFASVITASICPQAAGSGIPEVKAYLNGVDAPGILLPRTVLVKIIGSISI 208
           VFS SNLVLTLFASVITA ICPQAAGSGIPEVKAYLNGVDAPGIL PRT+LVKIIGSISI
Sbjct: 122 VFSFSNLVLTLFASVITAFICPQAAGSGIPEVKAYLNGVDAPGILSPRTLLVKIIGSISI 181

Query: 209 VSSSLIVGKAGPMVHTGACVASLVGQGGFKIFGLTWRSLCHLKNDRDRRDLVTCGAAAGI 268
           VSSSLIVGKAGPMVHTGACVASLVGQGGFK+FGLTWR L HLKNDRDRRDLVTCGAAAGI
Sbjct: 182 VSSSLIVGKAGPMVHTGACVASLVGQGGFKVFGLTWRWLHHLKNDRDRRDLVTCGAAAGI 241

Query: 269 AAAFRAPVGGVLFALEEMASWWRSALLWRSFFTTAVVAVVLRALIDVCLNGLCGLFGKGG 328
           AAAFRAPVGGVLFA EEMASWWRSALLWRSFFTTA+VAV+LR LID CLNGLCGLFGKGG
Sbjct: 242 AAAFRAPVGGVLFAFEEMASWWRSALLWRSFFTTAIVAVILRTLIDFCLNGLCGLFGKGG 301

Query: 329 LIIFDTYSDSPTYHLEDLPPVLALAIVGGILGS-----------------ERH-VYKIFL 388
           LIIFDTYSD P YHL DLPPVLALA +GGILGS                 ER  VYKI L
Sbjct: 302 LIIFDTYSDFPAYHLGDLPPVLALAFLGGILGSLYNFLLNKVLRVYNLIHERGIVYKILL 361

Query: 389 ACSVSIFTSCLLFGLPWFAPCQPCPSSSRETCPTIGRSGNFKKFRCAPGHYNDLASLIFN 448
           ACSVSIFTSCLLFGLPW A CQPCPSS+RE CPTIGRSGNFKKF+C+P HYNDLASLIFN
Sbjct: 362 ACSVSIFTSCLLFGLPWSASCQPCPSSAREICPTIGRSGNFKKFQCSPSHYNDLASLIFN 421

Query: 449 TNDDAIKNLFSKGTDSEFQFSSMLTFFITCFSLSILSYGTVVPAGLFVPVIVTGASYGRF 508
           TNDDAIKNLFSK TDSEFQFSSMLTFFITCFSLSILSYG V PAGLFVPVIVTGASYGRF
Sbjct: 422 TNDDAIKNLFSKDTDSEFQFSSMLTFFITCFSLSILSYGIVAPAGLFVPVIVTGASYGRF 481

Query: 509 VGMVVGPYTNLNHGFFAILGAASFLGGSMRTTVSLCVILLELTNNLLLLPLIMLVLLVSK 568
           VGMVVG YTNL+HGFFAILGAASFLGGSMRTTVSLCVI+LELTNNLLLLPL+MLVLLVSK
Sbjct: 482 VGMVVGRYTNLSHGFFAILGAASFLGGSMRTTVSLCVIMLELTNNLLLLPLVMLVLLVSK 541

Query: 569 TVADAFNGNIYNLIMKAKGFPYLEGHVEPYMRQLTVADVLTSPLQLLNGIEKVRKVVNVL 628
           TVADAFN NIYN IMKAKGFPYLEGHVEPYMRQLTVADVLTSPLQLL+GIEKVR VV+VL
Sbjct: 542 TVADAFNSNIYNSIMKAKGFPYLEGHVEPYMRQLTVADVLTSPLQLLHGIEKVRNVVSVL 601

Query: 629 KWTNHHGFPVIDEPPFSEFPVLYGLILRAHLLMLLKKKAFLSIPAPAPAPEGEDAFKLFS 688
           KWT+HHGFPVIDEPPFSEFP+LYGLILRAHL+MLLKKKAF S  APAPA E +DAFKLFS
Sbjct: 602 KWTSHHGFPVIDEPPFSEFPILYGLILRAHLIMLLKKKAFFS--APAPAQERDDAFKLFS 661

Query: 689 ADDFVKMGSGDIDRIEDVQLTDEEMDMYIDLHPFANTSPCTVLETTSLAKALEVFRET 729
            DDF KM SGD+ RIEDVQLTDEEM+M+IDLHPF NTSPCTVLET SLAKALE+FRET
Sbjct: 662 VDDFAKMDSGDVARIEDVQLTDEEMEMFIDLHPFTNTSPCTVLETMSLAKALEIFRET 717

BLAST of Sgr030558 vs. ExPASy TrEMBL
Match: A0A6J1HKQ6 (putative chloride channel-like protein CLC-g OS=Cucurbita maxima OX=3661 GN=LOC111465440 PE=3 SV=1)

HSP 1 Score: 1198.7 bits (3100), Expect = 0.0e+00
Identity = 608/719 (84.56%), Postives = 656/719 (91.24%), Query Frame = 0

Query: 30  AAVSTNGDEESIITPLLPPQKAVANSSSQVAIVGANVCPIESLDYEIFDNEFFMQDWRSR 89
           AA  +NGDEESIITPLL PQK++ NSSSQVA+VGAN+CPIESLDYEIFDN+FFMQDWRSR
Sbjct: 3   AANPSNGDEESIITPLLAPQKSLVNSSSQVALVGANICPIESLDYEIFDNDFFMQDWRSR 62

Query: 90  EDFQIFQYLIMKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRYATAFLV 149
           EDFQIFQYL++KWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRY  AFLV
Sbjct: 63  EDFQIFQYLVIKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRYWMAFLV 122

Query: 150 FSVSNLVLTLFASVITASICPQAAGSGIPEVKAYLNGVDAPGILLPRTVLVKIIGSISIV 209
           FSVSNL+LTLFASVITA ICP AAGSGIPEVKAYLNGVDAPGIL PRT+LVKIIGSI+IV
Sbjct: 123 FSVSNLLLTLFASVITALICPLAAGSGIPEVKAYLNGVDAPGILSPRTLLVKIIGSIAIV 182

Query: 210 SSSLIVGKAGPMVHTGACVASLVGQGGFKIFGLTWRSLCHLKNDRDRRDLVTCGAAAGIA 269
           SSS++VGKAGPMVHTGACVASLVGQG FK+FGLTWR L HLK DRDRRDLVTCGAAAGIA
Sbjct: 183 SSSMVVGKAGPMVHTGACVASLVGQGCFKVFGLTWRWLYHLKKDRDRRDLVTCGAAAGIA 242

Query: 270 AAFRAPVGGVLFALEEMASWWRSALLWRSFFTTAVVAVVLRALIDVCLNGLCGLFGKGGL 329
           AAFRAPVGGVLFA EEMASWWRSALLWR+FFTTA+VAV+LR+LIDVCLNGLCGLFGKGGL
Sbjct: 243 AAFRAPVGGVLFAFEEMASWWRSALLWRAFFTTAIVAVILRSLIDVCLNGLCGLFGKGGL 302

Query: 330 IIFDTYSDSPTYHLEDLPPVLALAIVGGILGS------------------ERHVYKIFLA 389
           IIFDTYSD P+YHL+DLPPVL+LA +GGILGS                  +  VYK+ LA
Sbjct: 303 IIFDTYSDFPSYHLKDLPPVLSLAFIGGILGSFYNFLLTKVLRVYNLIHEKGIVYKVLLA 362

Query: 390 CSVSIFTSCLLFGLPWFAPCQPCPSSSRETCPTIGRSGNFKKFRCAPGHYNDLASLIFNT 449
           CSVSIFTSCLLFGLPWFA CQPCPSS+RE CPTIGRSGNFKKF+C+P HYNDLASLIFNT
Sbjct: 363 CSVSIFTSCLLFGLPWFASCQPCPSSAREICPTIGRSGNFKKFQCSPDHYNDLASLIFNT 422

Query: 450 NDDAIKNLFSKGTDSEFQFSSMLTFFITCFSLSILSYGTVVPAGLFVPVIVTGASYGRFV 509
           NDDAIKNLFSKGT+SEFQFSSMLTFF+TCFSLS+LSYGTV P GLFVPVIVTGASYGRFV
Sbjct: 423 NDDAIKNLFSKGTESEFQFSSMLTFFVTCFSLSVLSYGTVAPVGLFVPVIVTGASYGRFV 482

Query: 510 GMVVGPYTNLNHGFFAILGAASFLGGSMRTTVSLCVILLELTNNLLLLPLIMLVLLVSKT 569
           GMVVGPYTNL+HGFFAILGAASFLGGSMRTTVSLCVI+LELTNNLLLLPL+MLVLL+SKT
Sbjct: 483 GMVVGPYTNLSHGFFAILGAASFLGGSMRTTVSLCVIMLELTNNLLLLPLVMLVLLISKT 542

Query: 570 VADAFNGNIYNLIMKAKGFPYLEGHVEPYMRQLTVADVLTSPLQLLNGIEKVRKVVNVLK 629
           VADAFN NIYN IMKAKGFPYLEGHVEPYMRQLTVA VLTSPLQL  GIEKVR VVNVLK
Sbjct: 543 VADAFNCNIYNQIMKAKGFPYLEGHVEPYMRQLTVASVLTSPLQLFCGIEKVRNVVNVLK 602

Query: 630 WTNHHGFPVIDEPPFSEFPVLYGLILRAHLLMLLKKKAFLSIPAPAPAPEGEDAFKLFSA 689
           WT+HHGFPV+DEPPFSEFPVLYGLILRAHL++LLKKKAFLS+  P    + +DA KL SA
Sbjct: 603 WTSHHGFPVVDEPPFSEFPVLYGLILRAHLIVLLKKKAFLSV--PTLESDRKDALKLLSA 662

Query: 690 DDFVKMGSGDIDRIEDVQLTDEEMDMYIDLHPFANTSPCTVLETTSLAKALEVFRETDV 731
           DDF  MGSGD++RIED+QLTDEEM+M+IDLHPFANTSPCTVLET SLAKA  +FRET +
Sbjct: 663 DDFAMMGSGDVNRIEDIQLTDEEMEMFIDLHPFANTSPCTVLETMSLAKAFAIFRETSL 719

BLAST of Sgr030558 vs. ExPASy TrEMBL
Match: A0A6J1FCB8 (putative chloride channel-like protein CLC-g OS=Cucurbita moschata OX=3662 GN=LOC111444324 PE=3 SV=1)

HSP 1 Score: 1198.3 bits (3099), Expect = 0.0e+00
Identity = 611/717 (85.22%), Postives = 653/717 (91.07%), Query Frame = 0

Query: 30  AAVSTNGDEESIITPLLPPQKAVANSSSQVAIVGANVCPIESLDYEIFDNEFFMQDWRSR 89
           AA  +NGDEESIITPLL PQK++ NSSSQVA+VGAN+CPIESLDYEIFDN+FFMQDWRSR
Sbjct: 3   AANPSNGDEESIITPLLAPQKSLVNSSSQVALVGANICPIESLDYEIFDNDFFMQDWRSR 62

Query: 90  EDFQIFQYLIMKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRYATAFLV 149
           EDFQIFQYL++KWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRY  AFLV
Sbjct: 63  EDFQIFQYLVIKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRYWMAFLV 122

Query: 150 FSVSNLVLTLFASVITASICPQAAGSGIPEVKAYLNGVDAPGILLPRTVLVKIIGSISIV 209
           FSVSNL+LTLFASVITA ICP AAGSGIPEVKAYLNGVDAPGIL PRT+LVKIIGSI+IV
Sbjct: 123 FSVSNLLLTLFASVITALICPLAAGSGIPEVKAYLNGVDAPGILSPRTLLVKIIGSIAIV 182

Query: 210 SSSLIVGKAGPMVHTGACVASLVGQGGFKIFGLTWRSLCHLKNDRDRRDLVTCGAAAGIA 269
           SSS++VGKAGPMVHTGACVASLVGQG FK+FGLTWR L HLK DRDRRDLVTCGAAAGIA
Sbjct: 183 SSSMVVGKAGPMVHTGACVASLVGQGCFKVFGLTWRWLYHLKKDRDRRDLVTCGAAAGIA 242

Query: 270 AAFRAPVGGVLFALEEMASWWRSALLWRSFFTTAVVAVVLRALIDVCLNGLCGLFGKGGL 329
           AAFRAPVGGVLFA EEMASWWRSALLWR+FFTTA+VAVVLR+LIDVCLNGLCGLFGKGGL
Sbjct: 243 AAFRAPVGGVLFAFEEMASWWRSALLWRAFFTTAIVAVVLRSLIDVCLNGLCGLFGKGGL 302

Query: 330 IIFDTYSDSPTYHLEDLPPVLALAIVGGILGS------------------ERHVYKIFLA 389
           IIFDTYSD P+YHL+DLPPVLALA +GGILGS                  +  VYK+ LA
Sbjct: 303 IIFDTYSDFPSYHLKDLPPVLALAFIGGILGSFYNFLLTKVLRVYNLIHEKGIVYKVLLA 362

Query: 390 CSVSIFTSCLLFGLPWFAPCQPCPSSSRETCPTIGRSGNFKKFRCAPGHYNDLASLIFNT 449
           CSVSIFTSCLLFGLPWFA CQPCPSS+RE CPTIGRSGNFKKF+C+ GHYNDLASLIFNT
Sbjct: 363 CSVSIFTSCLLFGLPWFASCQPCPSSAREICPTIGRSGNFKKFQCSYGHYNDLASLIFNT 422

Query: 450 NDDAIKNLFSKGTDSEFQFSSMLTFFITCFSLSILSYGTVVPAGLFVPVIVTGASYGRFV 509
           NDDAIKNLFSKGTDSEFQF SMLTFF+TCFSLS+LSYGTV P GLFVPVIVTGASYGRFV
Sbjct: 423 NDDAIKNLFSKGTDSEFQFLSMLTFFVTCFSLSVLSYGTVAPVGLFVPVIVTGASYGRFV 482

Query: 510 GMVVGPYTNLNHGFFAILGAASFLGGSMRTTVSLCVILLELTNNLLLLPLIMLVLLVSKT 569
           GMVVGPYTNL+HGFFAILGAASFLGGSMRTTVSLCVI+LELTNNLLLLPL+MLVLL+SKT
Sbjct: 483 GMVVGPYTNLSHGFFAILGAASFLGGSMRTTVSLCVIMLELTNNLLLLPLVMLVLLISKT 542

Query: 570 VADAFNGNIYNLIMKAKGFPYLEGHVEPYMRQLTVADVLTSPLQLLNGIEKVRKVVNVLK 629
           VADAFN NIYN IMKAKGFPYLE HVEPYMRQLTVA VLTSPLQL  GIEKVR VVNVLK
Sbjct: 543 VADAFNCNIYNQIMKAKGFPYLECHVEPYMRQLTVASVLTSPLQLFRGIEKVRNVVNVLK 602

Query: 630 WTNHHGFPVIDEPPFSEFPVLYGLILRAHLLMLLKKKAFLSIPAPAPAPEGEDAFKLFSA 689
           WT+HHGFP+IDEPPFSEFPVLYGLILRAHL++LLKKKAFLS+  P    E +DA KL SA
Sbjct: 603 WTSHHGFPIIDEPPFSEFPVLYGLILRAHLIVLLKKKAFLSV--PTLGLERQDALKLLSA 662

Query: 690 DDFVKMGSGDIDRIEDVQLTDEEMDMYIDLHPFANTSPCTVLETTSLAKALEVFRET 729
           DDF  MGSGD+DRIED+QLTDEEM+M+IDLHPFANTSPCTVLET SLAKA  +FRET
Sbjct: 663 DDFAMMGSGDVDRIEDIQLTDEEMEMFIDLHPFANTSPCTVLETMSLAKAFAIFRET 717

BLAST of Sgr030558 vs. ExPASy TrEMBL
Match: A0A1S3C4Y8 (putative chloride channel-like protein CLC-g OS=Cucumis melo OX=3656 GN=LOC103496902 PE=3 SV=1)

HSP 1 Score: 1196.4 bits (3094), Expect = 0.0e+00
Identity = 619/718 (86.21%), Postives = 654/718 (91.09%), Query Frame = 0

Query: 29  AAAVSTNGDEESIITPLLPPQKAVANSSSQVAIVGANVCPIESLDYEIFDNEFFMQDWRS 88
           A A S+NGDEESIITPLL  QK++ANSSSQVAIVGANVCPIESLDYEIFDNE FMQDWRS
Sbjct: 2   ALADSSNGDEESIITPLL-AQKSLANSSSQVAIVGANVCPIESLDYEIFDNECFMQDWRS 61

Query: 89  REDFQIFQYLIMKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRYATAFL 148
           R DFQIFQYL+MKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRY  AFL
Sbjct: 62  RGDFQIFQYLVMKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRYGMAFL 121

Query: 149 VFSVSNLVLTLFASVITASICPQAAGSGIPEVKAYLNGVDAPGILLPRTVLVKIIGSISI 208
           VFSVSNLVLTLFASVITA ICPQAAGSGIPEVKAYLNGVDAPGIL PRT+LVKI+GSISI
Sbjct: 122 VFSVSNLVLTLFASVITALICPQAAGSGIPEVKAYLNGVDAPGILSPRTLLVKIVGSISI 181

Query: 209 VSSSLIVGKAGPMVHTGACVASLVGQGGFKIFGLTWRSLCHLKNDRDRRDLVTCGAAAGI 268
           VSSS+IVGKAGPMVHTGACVASLVGQGGFKIFG TWR L  LKNDRDRRDLVTCGAAAGI
Sbjct: 182 VSSSMIVGKAGPMVHTGACVASLVGQGGFKIFGSTWRWLYLLKNDRDRRDLVTCGAAAGI 241

Query: 269 AAAFRAPVGGVLFALEEMASWWRSALLWRSFFTTAVVAVVLRALIDVCLNGLCGLFGKGG 328
           AA+FRAPVGGVLFA EEMASWWRSALLWRSFFTTAVVAVVLR+LID+CLNGLCGLFGKGG
Sbjct: 242 AASFRAPVGGVLFAFEEMASWWRSALLWRSFFTTAVVAVVLRSLIDMCLNGLCGLFGKGG 301

Query: 329 LIIFDTYSDSPTYHLEDLPPVLALAIVGGILGS------------------ERHVYKIFL 388
           LIIFDTYSD PTYHLEDLPPVL LA +GGILGS                  +  +YKI L
Sbjct: 302 LIIFDTYSDFPTYHLEDLPPVLVLAFIGGILGSLYNFLLNKFLRIYNFIHEKGIIYKILL 361

Query: 389 ACSVSIFTSCLLFGLPWFAPCQPCPSSSRETCPTIGRSGNFKKFRCAPGHYNDLASLIFN 448
           AC+VSIFTS LLFGLPWFA CQPCPSS+RE CPTIGRSGNFKKF+CAP HYNDLASLIFN
Sbjct: 362 ACTVSIFTSFLLFGLPWFASCQPCPSSAREICPTIGRSGNFKKFQCAPDHYNDLASLIFN 421

Query: 449 TNDDAIKNLFSKGTDSEFQFSSMLTFFITCFSLSILSYGTVVPAGLFVPVIVTGASYGRF 508
           TNDDAIKNLFSK TDSEFQ SSMLTFF+TCFSLSILSYGTV P GLFVPVIVTGASYGRF
Sbjct: 422 TNDDAIKNLFSKDTDSEFQLSSMLTFFVTCFSLSILSYGTVAPVGLFVPVIVTGASYGRF 481

Query: 509 VGMVVGPYTNLNHGFFAILGAASFLGGSMRTTVSLCVILLELTNNLLLLPLIMLVLLVSK 568
           VGMVVGP+TNL+HGFFAILGAASFLGG+MRTTVSLCVI+LELTNNLLLLPLIMLVLL+SK
Sbjct: 482 VGMVVGPHTNLSHGFFAILGAASFLGGTMRTTVSLCVIMLELTNNLLLLPLIMLVLLISK 541

Query: 569 TVADAFNGNIYNLIMKAKGFPYLEGHVEPYMRQLTVADVLTSPLQLLNGIEKVRKVVNVL 628
           TVADAFN NIYNLIMKAKGFPYLEGHVEPYMRQL VADVLTSPLQLLNGIEKV  VVNVL
Sbjct: 542 TVADAFNSNIYNLIMKAKGFPYLEGHVEPYMRQLKVADVLTSPLQLLNGIEKVCNVVNVL 601

Query: 629 KWTNHHGFPVIDEPPFSEFPVLYGLILRAHLLMLLKKKAFLSIPAPAPAPEGEDAFKLFS 688
           + T+HHGFP+IDEPPFSEFP+LYGL+LR HL+MLLKKKAFLS+  P P  E +D  KLFS
Sbjct: 602 RMTSHHGFPIIDEPPFSEFPILYGLVLRTHLIMLLKKKAFLSV--PIPGSEIQDPCKLFS 661

Query: 689 ADDFVKMGSGDIDRIEDVQLTDEEMDMYIDLHPFANTSPCTVLETTSLAKALEVFRET 729
           ADDF KMGSG ++RIEDVQLTDEEM+M+IDLHPFAN SPCTVLETTSLAKALE+FRET
Sbjct: 662 ADDFAKMGSGYVERIEDVQLTDEEMEMFIDLHPFANPSPCTVLETTSLAKALEIFRET 716

BLAST of Sgr030558 vs. ExPASy TrEMBL
Match: A0A5A7V604 (Putative chloride channel-like protein CLC-g OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G00620 PE=3 SV=1)

HSP 1 Score: 1196.0 bits (3093), Expect = 0.0e+00
Identity = 619/718 (86.21%), Postives = 654/718 (91.09%), Query Frame = 0

Query: 29  AAAVSTNGDEESIITPLLPPQKAVANSSSQVAIVGANVCPIESLDYEIFDNEFFMQDWRS 88
           A A S+NGDEESIITPLL  QK++ANSSSQVAIVGANVCPIESLDYEIFDNE FMQDWRS
Sbjct: 2   ALADSSNGDEESIITPLL-AQKSLANSSSQVAIVGANVCPIESLDYEIFDNECFMQDWRS 61

Query: 89  REDFQIFQYLIMKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRYATAFL 148
           R DFQIFQYL+MKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRY  AFL
Sbjct: 62  RGDFQIFQYLVMKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRYGMAFL 121

Query: 149 VFSVSNLVLTLFASVITASICPQAAGSGIPEVKAYLNGVDAPGILLPRTVLVKIIGSISI 208
           VFSVSNLVLTLFASVITA ICPQAAGSGIPEVKAYLNGVDAPGIL PRT+LVKI+GSISI
Sbjct: 122 VFSVSNLVLTLFASVITALICPQAAGSGIPEVKAYLNGVDAPGILSPRTLLVKIVGSISI 181

Query: 209 VSSSLIVGKAGPMVHTGACVASLVGQGGFKIFGLTWRSLCHLKNDRDRRDLVTCGAAAGI 268
           VSSS+IVGKAGPMVHTGACVASLVGQGGFKIFG TWR L  LKNDRDRRDLVTCGAAAGI
Sbjct: 182 VSSSMIVGKAGPMVHTGACVASLVGQGGFKIFGSTWRWLYLLKNDRDRRDLVTCGAAAGI 241

Query: 269 AAAFRAPVGGVLFALEEMASWWRSALLWRSFFTTAVVAVVLRALIDVCLNGLCGLFGKGG 328
           AA+FRAPVGGVLFA EEMASWWRSALLWRSFFTTAVVAVVLR+LID+CLNGLCGLFGKGG
Sbjct: 242 AASFRAPVGGVLFAFEEMASWWRSALLWRSFFTTAVVAVVLRSLIDMCLNGLCGLFGKGG 301

Query: 329 LIIFDTYSDSPTYHLEDLPPVLALAIVGGILGS------------------ERHVYKIFL 388
           LIIFDTYSD PTYHLEDLPPVL LA +GGILGS                  +  +YKI L
Sbjct: 302 LIIFDTYSDFPTYHLEDLPPVLVLAFIGGILGSLYNFLLNKFLRIYNFIHEKGIIYKILL 361

Query: 389 ACSVSIFTSCLLFGLPWFAPCQPCPSSSRETCPTIGRSGNFKKFRCAPGHYNDLASLIFN 448
           AC+VSIFTS LLFGLPWFA CQPCPSS+RE CPTIGRSGNFKKF+CAP HYNDLASLIFN
Sbjct: 362 ACTVSIFTSFLLFGLPWFASCQPCPSSAREICPTIGRSGNFKKFQCAPDHYNDLASLIFN 421

Query: 449 TNDDAIKNLFSKGTDSEFQFSSMLTFFITCFSLSILSYGTVVPAGLFVPVIVTGASYGRF 508
           TNDDAIKNLFSK TDSEFQ SSMLTFF+TCFSLSILSYGTV P GLFVPVIVTGASYGRF
Sbjct: 422 TNDDAIKNLFSKDTDSEFQLSSMLTFFVTCFSLSILSYGTVAPVGLFVPVIVTGASYGRF 481

Query: 509 VGMVVGPYTNLNHGFFAILGAASFLGGSMRTTVSLCVILLELTNNLLLLPLIMLVLLVSK 568
           VGMVVGP+TNL+HGFFAILGAASFLGG+MRTTVSLCVI+LELTNNLLLLPLIMLVLL+SK
Sbjct: 482 VGMVVGPHTNLSHGFFAILGAASFLGGTMRTTVSLCVIMLELTNNLLLLPLIMLVLLISK 541

Query: 569 TVADAFNGNIYNLIMKAKGFPYLEGHVEPYMRQLTVADVLTSPLQLLNGIEKVRKVVNVL 628
           TVADAFN NIYNLIMKAKGFPYLEGHVEPYMRQL VADVLTSPLQLLNGIEKV  VVNVL
Sbjct: 542 TVADAFNSNIYNLIMKAKGFPYLEGHVEPYMRQLKVADVLTSPLQLLNGIEKVCNVVNVL 601

Query: 629 KWTNHHGFPVIDEPPFSEFPVLYGLILRAHLLMLLKKKAFLSIPAPAPAPEGEDAFKLFS 688
           + T+HHGFP+IDEPPFSEFP+LYGL+LR HL+MLLKKKAFLS+  P    E +D  KLFS
Sbjct: 602 RMTSHHGFPIIDEPPFSEFPILYGLVLRTHLIMLLKKKAFLSV--PILGSEIQDPCKLFS 661

Query: 689 ADDFVKMGSGDIDRIEDVQLTDEEMDMYIDLHPFANTSPCTVLETTSLAKALEVFRET 729
           ADDF KMGSGD++RIEDVQLTDEEM+M+IDLHPFAN SPCTVLETTSLAKALE+FRET
Sbjct: 662 ADDFAKMGSGDVERIEDVQLTDEEMEMFIDLHPFANPSPCTVLETTSLAKALEIFRET 716

BLAST of Sgr030558 vs. TAIR 10
Match: AT5G33280.1 (Voltage-gated chloride channel family protein )

HSP 1 Score: 977.2 bits (2525), Expect = 7.5e-285
Identity = 497/711 (69.90%), Postives = 582/711 (81.86%), Query Frame = 0

Query: 38  EESIITPLLPPQKAVANSSSQVAIVGANVCPIESLDYEIFDNEFFMQDWRSREDFQIFQY 97
           E+S+  PLLP  +   NS+SQVAIVGANVCPIESLDYEI +N+FF QDWR R   +IFQY
Sbjct: 7   EDSVAVPLLPSLRRATNSTSQVAIVGANVCPIESLDYEIAENDFFKQDWRGRSKVEIFQY 66

Query: 98  LIMKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRYATAFLVFSVSNLVL 157
           + MKWL CF IG+I+ L+GF NNLAVEN+AG KFVVTSNMM+ GR+A  F+VFSV+NL+L
Sbjct: 67  VFMKWLLCFCIGIIVSLIGFANNLAVENLAGVKFVVTSNMMIAGRFAMGFVVFSVTNLIL 126

Query: 158 TLFASVITASICPQAAGSGIPEVKAYLNGVDAPGILLPRTVLVKIIGSISIVSSSLIVGK 217
           TLFASVITA + P AAGSGIPEVKAYLNGVDAP I   RT+++KIIG+IS VS+SL++GK
Sbjct: 127 TLFASVITAFVAPAAAGSGIPEVKAYLNGVDAPEIFSLRTLIIKIIGNISAVSASLLIGK 186

Query: 218 AGPMVHTGACVASLVGQGGFKIFGLTWRSLCHLKNDRDRRDLVTCGAAAGIAAAFRAPVG 277
           AGPMVHTGACVAS++GQGG K + LTWR L   KNDRDRRDLVTCGAAAGIAA+FRAPVG
Sbjct: 187 AGPMVHTGACVASILGQGGSKRYRLTWRWLRFFKNDRDRRDLVTCGAAAGIAASFRAPVG 246

Query: 278 GVLFALEEMASWWRSALLWRSFFTTAVVAVVLRALIDVCLNGLCGLFGKGGLIIFDTYSD 337
           GVLFALEEM+SWWRSALLWR FF+TAVVA+VLRALIDVCL+G CGLFGKGGLI+FD YS+
Sbjct: 247 GVLFALEEMSSWWRSALLWRIFFSTAVVAIVLRALIDVCLSGKCGLFGKGGLIMFDVYSE 306

Query: 338 SPTYHLEDLPPVLALAIVGGILGS-----------------ERHV-YKIFLACSVSIFTS 397
           + +YHL D+ PVL L +VGGILGS                 E+ V +KI LAC++SIFTS
Sbjct: 307 NASYHLGDVLPVLLLGVVGGILGSLYNFLLDKVLRAYNYIYEKGVTWKILLACAISIFTS 366

Query: 398 CLLFGLPWFAPCQPCPSSSRETCPTIGRSGNFKKFRCAPGHYNDLASLIFNTNDDAIKNL 457
           CLLFGLP+ A CQPCP  + E CPTIGRSGNFKK++C PGHYNDLASLIFNTNDDAIKNL
Sbjct: 367 CLLFGLPFLASCQPCPVDALEECPTIGRSGNFKKYQCPPGHYNDLASLIFNTNDDAIKNL 426

Query: 458 FSKGTDSEFQFSSMLTFFITCFSLSILSYGTVVPAGLFVPVIVTGASYGRFVGMVVGPYT 517
           FSK TD EF + S+L FF+TCF LSI SYG V PAGLFVPVIVTGASYGRFVGM++G  +
Sbjct: 427 FSKNTDFEFHYFSVLVFFVTCFFLSIFSYGIVAPAGLFVPVIVTGASYGRFVGMLLGSNS 486

Query: 518 NLNHGFFAILGAASFLGGSMRTTVSLCVILLELTNNLLLLPLIMLVLLVSKTVADAFNGN 577
           NLNHG FA+LGAASFLGG+MR TVS CVILLELTNNLLLLP++M+VLL+SKTVAD FN N
Sbjct: 487 NLNHGLFAVLGAASFLGGTMRMTVSTCVILLELTNNLLLLPMMMVVLLISKTVADGFNAN 546

Query: 578 IYNLIMKAKGFPYLEGHVEPYMRQLTVADVLTSPLQLLNGIEKVRKVVNVLKWTNHHGFP 637
           IYNLIMK KGFPYL  H EPYMRQL V DV+T PLQ+ NGIEKV  +V+VLK TNH+GFP
Sbjct: 547 IYNLIMKLKGFPYLYSHAEPYMRQLLVGDVVTGPLQVFNGIEKVETIVHVLKTTNHNGFP 606

Query: 638 VIDEPPFSEFPVLYGLILRAHLLMLLKKKAFLSIPAPAPAPEGEDAFKLFSADDFVKMGS 697
           V+D PP +  PVL+GLILRAH+L LLKK+ F+    P+P     +    F A++F K GS
Sbjct: 607 VVDGPPLAAAPVLHGLILRAHILTLLKKRVFM----PSPVACDSNTLSQFKAEEFAKKGS 666

Query: 698 GDIDRIEDVQLTDEEMDMYIDLHPFANTSPCTVLETTSLAKALEVFRETDV 731
           G  D+IEDV+L++EE++MY+DLHPF+N SP TV+ET SLAKAL +FRE  +
Sbjct: 667 GRSDKIEDVELSEEELNMYLDLHPFSNASPYTVVETMSLAKALILFREVGI 713

BLAST of Sgr030558 vs. TAIR 10
Match: AT5G49890.1 (chloride channel C )

HSP 1 Score: 770.8 bits (1989), Expect = 1.1e-222
Identity = 398/693 (57.43%), Postives = 499/693 (72.01%), Query Frame = 0

Query: 54  NSSSQVAIVGANVCPIESLDYEIFDNEFFMQDWRSREDFQIFQYLIMKWLSCFLIGLIMG 113
           N++SQ+AIVGAN CPIESLDYEIF+N+FF QDWRSR+  +I QY  +KW   FLIGL  G
Sbjct: 48  NTTSQIAIVGANTCPIESLDYEIFENDFFKQDWRSRKKIEILQYTFLKWALAFLIGLATG 107

Query: 114 LVGFFNNLAVENIAGKKFVVTSNMMLEGRYATAFLVFSVSNLVLTLFASVITASICPQAA 173
           LVGF NNL VENIAG K ++  N+ML+ +Y  AF  F+  NL+L   A+ + A I P AA
Sbjct: 108 LVGFLNNLGVENIAGFKLLLIGNLMLKEKYFQAFFAFAGCNLILATAAASLCAFIAPAAA 167

Query: 174 GSGIPEVKAYLNGVDAPGILLPRTVLVKIIGSISIVSSSLIVGKAGPMVHTGACVASLVG 233
           GSGIPEVKAYLNG+DA  IL P T+ VKI GSI  V++  +VGK GPMVHTGAC+A+L+G
Sbjct: 168 GSGIPEVKAYLNGIDAYSILAPSTLFVKIFGSIFGVAAGFVVGKEGPMVHTGACIANLLG 227

Query: 234 QGGFKIFGLTWRSLCHLKNDRDRRDLVTCGAAAGIAAAFRAPVGGVLFALEEMASWWRSA 293
           QGG K + LTW+ L   KNDRDRRDL+TCGAAAG+AAAFRAPVGGVLFALEE ASWWR+A
Sbjct: 228 QGGSKKYRLTWKWLRFFKNDRDRRDLITCGAAAGVAAAFRAPVGGVLFALEEAASWWRNA 287

Query: 294 LLWRSFFTTAVVAVVLRALIDVCLNGLCGLFGKGGLIIFDTYSDSPTYHLEDLPPVLALA 353
           LLWR+FFTTAVVAVVLR+LI+ C +G CGLFGKGGLI+FD  S    Y   DL  ++ L 
Sbjct: 288 LLWRTFFTTAVVAVVLRSLIEFCRSGRCGLFGKGGLIMFDVNSGPVLYSTPDLLAIVFLG 347

Query: 354 IVGGILGSERHV------------------YKIFLACSVSIFTSCLLFGLPWFAPCQPCP 413
           ++GG+LGS  +                   +KI L  +VSI +SC  FGLPW + C PCP
Sbjct: 348 VIGGVLGSLYNYLVDKVLRTYSIINEKGPRFKIMLVMAVSILSSCCAFGLPWLSQCTPCP 407

Query: 414 SSSRE-TCPTIGRSGNFKKFRCAPGHYNDLASLIFNTNDDAIKNLFSKGTDSEFQFSSML 473
               E  CP++GRS  +K F+C P HYNDL+SL+ NTNDDAI+NLF+  +++EF  S++ 
Sbjct: 408 IGIEEGKCPSVGRSSIYKSFQCPPNHYNDLSSLLLNTNDDAIRNLFTSRSENEFHISTLA 467

Query: 474 TFFITCFSLSILSYGTVVPAGLFVPVIVTGASYGRFVGMVVGPYTNLNHGFFAILGAASF 533
            FF+  + L I++YG  +P+GLF+PVI+ GASYGR VG ++GP + L+ G F++LGAASF
Sbjct: 468 IFFVAVYCLGIITYGIAIPSGLFIPVILAGASYGRLVGRLLGPVSQLDVGLFSLLGAASF 527

Query: 534 LGGSMRTTVSLCVILLELTNNLLLLPLIMLVLLVSKTVADAFNGNIYNLIMKAKGFPYLE 593
           LGG+MR TVSLCVILLELTNNLL+LPL+MLVLL+SKTVAD FN  +Y+ I+  KG PY+E
Sbjct: 528 LGGTMRMTVSLCVILLELTNNLLMLPLVMLVLLISKTVADCFNRGVYDQIVTMKGLPYME 587

Query: 594 GHVEPYMRQLTVADVLTSPLQLLNGIEKVRKVVNVLKWTNHHGFPVIDEPPFSEFPVLYG 653
            H EPYMR L   DV++  L   + +EKV  +   LK T H+GFPVIDEPPF+E   L G
Sbjct: 588 DHAEPYMRNLVAKDVVSGALISFSRVEKVGVIWQALKMTRHNGFPVIDEPPFTEASELCG 647

Query: 654 LILRAHLLMLLKKKAFLSIPAPAPAPEGEDAFKLFSADDFVKMGSGDIDRIEDVQLTDEE 713
           + LR+HLL+LL+ K F    +      G    +   A DF K G G   +IED+ L++EE
Sbjct: 648 IALRSHLLVLLQGKKF----SKQRTTFGSQILRSCKARDFGKAGLGKGLKIEDLDLSEEE 707

Query: 714 MDMYIDLHPFANTSPCTVLETTSLAKALEVFRE 728
           M+MY+DLHP  NTSP TVLET SLAKA  +FR+
Sbjct: 708 MEMYVDLHPITNTSPYTVLETLSLAKAAILFRQ 736

BLAST of Sgr030558 vs. TAIR 10
Match: AT3G27170.1 (chloride channel B )

HSP 1 Score: 679.9 bits (1753), Expect = 2.5e-195
Identity = 359/720 (49.86%), Postives = 489/720 (67.92%), Query Frame = 0

Query: 36  GDEES--IITPLLPPQKAVANSSSQVAIVGANVCPIESLDYEIFDNEFFMQDWRSREDFQ 95
           GD ES  +  PL+   + +  SS+ +A+VGA V  IESLDYEI +N+ F  DWR R   Q
Sbjct: 20  GDPESNTLNQPLVKANRTL--SSTPLALVGAKVSHIESLDYEINENDLFKHDWRKRSKAQ 79

Query: 96  IFQYLIMKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRYATAFLVFSVS 155
           + QY+ +KW    L+GL  GL+    NLAVENIAG K +   + + + RY T  +V   +
Sbjct: 80  VLQYVFLKWTLACLVGLFTGLIATLINLAVENIAGYKLLAVGHFLTQERYVTGLMVLVGA 139

Query: 156 NLVLTLFASVITASICPQAAGSGIPEVKAYLNGVDAPGILLPRTVLVKIIGSISIVSSSL 215
           NL LTL ASV+     P AAG GIPE+KAYLNGVD P +    T++VKI+GSI  V++ L
Sbjct: 140 NLGLTLVASVLCVCFAPTAAGPGIPEIKAYLNGVDTPNMFGATTMIVKIVGSIGAVAAGL 199

Query: 216 IVGKAGPMVHTGACVASLVGQGGFKIFGLTWRSLCHLKNDRDRRDLVTCGAAAGIAAAFR 275
            +GK GP+VH G+C+ASL+GQGG     + WR L +  NDRDRRDL+TCG+AAG+ AAFR
Sbjct: 200 DLGKEGPLVHIGSCIASLLGQGGTDNHRIKWRWLRYFNNDRDRRDLITCGSAAGVCAAFR 259

Query: 276 APVGGVLFALEEMASWWRSALLWRSFFTTAVVAVVLRALIDVCLNGLCGLFGKGGLIIFD 335
           +PVGGVLFALEE+A+WWRSALLWR+FF+TAVV VVLR  I++C +G CGLFGKGGLI+FD
Sbjct: 260 SPVGGVLFALEEVATWWRSALLWRTFFSTAVVVVVLREFIEICNSGKCGLFGKGGLIMFD 319

Query: 336 TYSDSPTYHLEDLPPVLALAIVGGILGS------------------ERHVYKIFLACSVS 395
               + TYH+ D+ PV+ + ++GGILGS                  +  ++K+ L+ +VS
Sbjct: 320 VSHVTYTYHVTDIIPVMLIGVIGGILGSLYNHLLHKVLRLYNLINEKGKIHKVLLSLTVS 379

Query: 396 IFTSCLLFGLPWFAPCQPCPSSSRETCPTIGRSGNFKKFRCAPGHYNDLASLIFNTNDDA 455
           +FTS  L+GLP+ A C+PC  S  E CPT GRSGNFK+F C  G+YNDLA+L+  TNDDA
Sbjct: 380 LFTSVCLYGLPFLAKCKPCDPSIDEICPTNGRSGNFKQFHCPKGYYNDLATLLLTTNDDA 439

Query: 456 IKNLFSKGTDSEFQFSSMLTFFITCFSLSILSYGTVVPAGLFVPVIVTGASYGRFVGMVV 515
           ++NLFS  T +EF   S+  FF+    L + ++G   P+GLF+P+I+ GA+YGR +G  +
Sbjct: 440 VRNLFSSNTPNEFGMGSLWIFFVLYCILGLFTFGIATPSGLFLPIILMGAAYGRMLGAAM 499

Query: 516 GPYTNLNHGFFAILGAASFLGGSMRTTVSLCVILLELTNNLLLLPLIMLVLLVSKTVADA 575
           G YT+++ G +A+LGAA+ + GSMR TVSLCVI LELTNNLLLLP+ M+VLL++KTV D+
Sbjct: 500 GSYTSIDQGLYAVLGAAALMAGSMRMTVSLCVIFLELTNNLLLLPITMIVLLIAKTVGDS 559

Query: 576 FNGNIYNLIMKAKGFPYLEGHVEPYMRQLTVADV--LTSPLQLLNGIEKVRKVVNVLKWT 635
           FN +IY++I+  KG P+LE + EP+MR LTV ++     P+  L G+EKV  +V+VLK T
Sbjct: 560 FNPSIYDIILHLKGLPFLEANPEPWMRNLTVGELGDAKPPVVTLQGVEKVSNIVDVLKNT 619

Query: 636 NHHGFPVIDEPPFSEFPV------LYGLILRAHLLMLLKKKAFLSIPAPAPAPEGEDAFK 695
            H+ FPV+DE    +  +      L+GLILRAHL+ +LKK+ FL+        E  +  +
Sbjct: 620 THNAFPVLDEAEVPQVGLATGATELHGLILRAHLVKVLKKRWFLT---EKRRTEEWEVRE 679

Query: 696 LFSADDFVKMGSGDIDRIEDVQLTDEEMDMYIDLHPFANTSPCTVLETTSLAKALEVFRE 728
            F  D+  +      D  +DV +T  EM+MY+DLHP  NT+P TV+E  S+AKAL +FR+
Sbjct: 680 KFPWDELAERE----DNFDDVAITSAEMEMYVDLHPLTNTTPYTVMENMSVAKALVLFRQ 730

BLAST of Sgr030558 vs. TAIR 10
Match: AT5G40890.1 (chloride channel A )

HSP 1 Score: 667.9 bits (1722), Expect = 9.7e-192
Identity = 355/719 (49.37%), Postives = 484/719 (67.32%), Query Frame = 0

Query: 35  NGDEE-------SIITPLLPPQKAVANSSSQVAIVGANVCPIESLDYEIFDNEFFMQDWR 94
           NG+EE       ++  PLL   + +  SS+ +A+VGA V  IESLDYEI +N+ F  DWR
Sbjct: 15  NGEEEGEDPENNTLNQPLLKRHRTL--SSTPLALVGAKVSHIESLDYEINENDLFKHDWR 74

Query: 95  SREDFQIFQYLIMKWLSCFLIGLIMGLVGFFNNLAVENIAGKKFVVTSNMMLEGRYATAF 154
           SR   Q+FQY+ +KW    L+GL  GL+    NLAVENIAG K +     + + R+ T  
Sbjct: 75  SRSKAQVFQYIFLKWTLACLVGLFTGLIATLINLAVENIAGYKLLAVGYYIAQDRFWTGL 134

Query: 155 LVFSVSNLVLTLFASVITASICPQAAGSGIPEVKAYLNGVDAPGILLPRTVLVKIIGSIS 214
           +VF+ +NL LTL A+V+     P AAG GIPE+KAYLNG+D P +    T++VKI+GSI 
Sbjct: 135 MVFTGANLGLTLVATVLVVYFAPTAAGPGIPEIKAYLNGIDTPNMFGFTTMMVKIVGSIG 194

Query: 215 IVSSSLIVGKAGPMVHTGACVASLVGQGGFKIFGLTWRSLCHLKNDRDRRDLVTCGAAAG 274
            V++ L +GK GP+VH G+C+ASL+GQGG     + WR L +  NDRDRRDL+TCG+A+G
Sbjct: 195 AVAAGLDLGKEGPLVHIGSCIASLLGQGGPDNHRIKWRWLRYFNNDRDRRDLITCGSASG 254

Query: 275 IAAAFRAPVGGVLFALEEMASWWRSALLWRSFFTTAVVAVVLRALIDVCLNGLCGLFGKG 334
           + AAFR+PVGGVLFALEE+A+WWRSALLWR+FF+TAVV VVLRA I++C +G CGLFG G
Sbjct: 255 VCAAFRSPVGGVLFALEEVATWWRSALLWRTFFSTAVVVVVLRAFIEICNSGKCGLFGSG 314

Query: 335 GLIIFDTYSDSPTYHLEDLPPVLALAIVGGILGS------------------ERHVYKIF 394
           GLI+FD       YH  D+ PV  + + GGILGS                  +  ++K+ 
Sbjct: 315 GLIMFDVSHVEVRYHAADIIPVTLIGVFGGILGSLYNHLLHKVLRLYNLINQKGKIHKVL 374

Query: 395 LACSVSIFTSCLLFGLPWFAPCQPCPSSSRETCPTIGRSGNFKKFRCAPGHYNDLASLIF 454
           L+  VS+FTS  LFGLP+ A C+PC  S  E CPT GRSGNFK+F C  G+YNDL++L+ 
Sbjct: 375 LSLGVSLFTSVCLFGLPFLAECKPCDPSIDEICPTNGRSGNFKQFNCPNGYYNDLSTLLL 434

Query: 455 NTNDDAIKNLFSKGTDSEFQFSSMLTFFITCFSLSILSYGTVVPAGLFVPVIVTGASYGR 514
            TNDDA++N+FS  T +EF   S+  FF     L ++++G   P+GLF+P+I+ G++YGR
Sbjct: 435 TTNDDAVRNIFSSNTPNEFGMVSLWIFFGLYCILGLITFGIATPSGLFLPIILMGSAYGR 494

Query: 515 FVGMVVGPYTNLNHGFFAILGAASFLGGSMRTTVSLCVILLELTNNLLLLPLIMLVLLVS 574
            +G  +G YTN++ G +A+LGAAS + GSMR TVSLCVI LELTNNLLLLP+ M VLL++
Sbjct: 495 MLGTAMGSYTNIDQGLYAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITMFVLLIA 554

Query: 575 KTVADAFNGNIYNLIMKAKGFPYLEGHVEPYMRQLTVADV--LTSPLQLLNGIEKVRKVV 634
           KTV D+FN +IY +I+  KG P+LE + EP+MR LTV ++     P+  LNG+EKV  +V
Sbjct: 555 KTVGDSFNLSIYEIILHLKGLPFLEANPEPWMRNLTVGELNDAKPPVVTLNGVEKVANIV 614

Query: 635 NVLKWTNHHGFPVIDEPPFSEFPVLYGLILRAHLLMLLKKKAFLSIPAPAPAPEGEDAFK 694
           +VL+ T H+ FPV+D    +    L+GLILRAHL+ +LKK+ FL+        E  + F 
Sbjct: 615 DVLRNTTHNAFPVLDGADQNTGTELHGLILRAHLVKVLKKRWFLNEKRRTEEWEVREKFT 674

Query: 695 LFSADDFVKMGSGDIDRIEDVQLTDEEMDMYIDLHPFANTSPCTVLETTSLAKALEVFR 727
                  V++   + D  +DV +T  EM +Y+DLHP  NT+P TV+++ S+AKAL +FR
Sbjct: 675 P------VELAERE-DNFDDVAITSSEMQLYVDLHPLTNTTPYTVVQSMSVAKALVLFR 724

BLAST of Sgr030558 vs. TAIR 10
Match: AT5G40890.2 (chloride channel A )

HSP 1 Score: 582.0 bits (1499), Expect = 7.0e-166
Identity = 303/599 (50.58%), Postives = 413/599 (68.95%), Query Frame = 0

Query: 148 LVFSVSNLVLTLFASVITASICPQAAGSGIPEVKAYLNGVDAPGILLPRTVLVKIIGSIS 207
           +VF+ +NL LTL A+V+     P AAG GIPE+KAYLNG+D P +    T++VKI+GSI 
Sbjct: 1   MVFTGANLGLTLVATVLVVYFAPTAAGPGIPEIKAYLNGIDTPNMFGFTTMMVKIVGSIG 60

Query: 208 IVSSSLIVGKAGPMVHTGACVASLVGQGGFKIFGLTWRSLCHLKNDRDRRDLVTCGAAAG 267
            V++ L +GK GP+VH G+C+ASL+GQGG     + WR L +  NDRDRRDL+TCG+A+G
Sbjct: 61  AVAAGLDLGKEGPLVHIGSCIASLLGQGGPDNHRIKWRWLRYFNNDRDRRDLITCGSASG 120

Query: 268 IAAAFRAPVGGVLFALEEMASWWRSALLWRSFFTTAVVAVVLRALIDVCLNGLCGLFGKG 327
           + AAFR+PVGGVLFALEE+A+WWRSALLWR+FF+TAVV VVLRA I++C +G CGLFG G
Sbjct: 121 VCAAFRSPVGGVLFALEEVATWWRSALLWRTFFSTAVVVVVLRAFIEICNSGKCGLFGSG 180

Query: 328 GLIIFDTYSDSPTYHLEDLPPVLALAIVGGILGS------------------ERHVYKIF 387
           GLI+FD       YH  D+ PV  + + GGILGS                  +  ++K+ 
Sbjct: 181 GLIMFDVSHVEVRYHAADIIPVTLIGVFGGILGSLYNHLLHKVLRLYNLINQKGKIHKVL 240

Query: 388 LACSVSIFTSCLLFGLPWFAPCQPCPSSSRETCPTIGRSGNFKKFRCAPGHYNDLASLIF 447
           L+  VS+FTS  LFGLP+ A C+PC  S  E CPT GRSGNFK+F C  G+YNDL++L+ 
Sbjct: 241 LSLGVSLFTSVCLFGLPFLAECKPCDPSIDEICPTNGRSGNFKQFNCPNGYYNDLSTLLL 300

Query: 448 NTNDDAIKNLFSKGTDSEFQFSSMLTFFITCFSLSILSYGTVVPAGLFVPVIVTGASYGR 507
            TNDDA++N+FS  T +EF   S+  FF     L ++++G   P+GLF+P+I+ G++YGR
Sbjct: 301 TTNDDAVRNIFSSNTPNEFGMVSLWIFFGLYCILGLITFGIATPSGLFLPIILMGSAYGR 360

Query: 508 FVGMVVGPYTNLNHGFFAILGAASFLGGSMRTTVSLCVILLELTNNLLLLPLIMLVLLVS 567
            +G  +G YTN++ G +A+LGAAS + GSMR TVSLCVI LELTNNLLLLP+ M VLL++
Sbjct: 361 MLGTAMGSYTNIDQGLYAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITMFVLLIA 420

Query: 568 KTVADAFNGNIYNLIMKAKGFPYLEGHVEPYMRQLTVADV--LTSPLQLLNGIEKVRKVV 627
           KTV D+FN +IY +I+  KG P+LE + EP+MR LTV ++     P+  LNG+EKV  +V
Sbjct: 421 KTVGDSFNLSIYEIILHLKGLPFLEANPEPWMRNLTVGELNDAKPPVVTLNGVEKVANIV 480

Query: 628 NVLKWTNHHGFPVIDEPPFSEFPVLYGLILRAHLLMLLKKKAFLSIPAPAPAPEGEDAFK 687
           +VL+ T H+ FPV+D    +    L+GLILRAHL+ +LKK+ FL+        E  + F 
Sbjct: 481 DVLRNTTHNAFPVLDGADQNTGTELHGLILRAHLVKVLKKRWFLNEKRRTEEWEVREKFT 540

Query: 688 LFSADDFVKMGSGDIDRIEDVQLTDEEMDMYIDLHPFANTSPCTVLETTSLAKALEVFR 727
                  V++   + D  +DV +T  EM +Y+DLHP  NT+P TV+++ S+AKAL +FR
Sbjct: 541 P------VELAERE-DNFDDVAITSSEMQLYVDLHPLTNTTPYTVVQSMSVAKALVLFR 592

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7021222.10.0e+0083.23putative chloride channel-like protein CLC-g [Cucurbita argyrosperma subsp. argy... [more]
XP_022143011.10.0e+0087.88putative chloride channel-like protein CLC-g isoform X1 [Momordica charantia][more]
XP_038890173.10.0e+0086.49putative chloride channel-like protein CLC-g isoform X1 [Benincasa hispida][more]
XP_023536768.10.0e+0085.36putative chloride channel-like protein CLC-g [Cucurbita pepo subsp. pepo][more]
KAG6586372.10.0e+0085.36putative chloride channel-like protein CLC-g, partial [Cucurbita argyrosperma su... [more]
Match NameE-valueIdentityDescription
P603001.1e-28369.90Putative chloride channel-like protein CLC-g OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q962821.5e-22157.43Chloride channel protein CLC-c OS=Arabidopsis thaliana OX=3702 GN=CLC-C PE=1 SV=... [more]
P929423.5e-19449.86Chloride channel protein CLC-b OS=Arabidopsis thaliana OX=3702 GN=CLC-B PE=1 SV=... [more]
P929411.4e-19049.37Chloride channel protein CLC-a OS=Arabidopsis thaliana OX=3702 GN=CLC-A PE=1 SV=... [more]
P929435.4e-15546.66Chloride channel protein CLC-d OS=Arabidopsis thaliana OX=3702 GN=CLC-D PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1CNX50.0e+0087.88putative chloride channel-like protein CLC-g isoform X1 OS=Momordica charantia O... [more]
A0A6J1HKQ60.0e+0084.56putative chloride channel-like protein CLC-g OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1FCB80.0e+0085.22putative chloride channel-like protein CLC-g OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A1S3C4Y80.0e+0086.21putative chloride channel-like protein CLC-g OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A5A7V6040.0e+0086.21Putative chloride channel-like protein CLC-g OS=Cucumis melo var. makuwa OX=1194... [more]
Match NameE-valueIdentityDescription
AT5G33280.17.5e-28569.90Voltage-gated chloride channel family protein [more]
AT5G49890.11.1e-22257.43chloride channel C [more]
AT3G27170.12.5e-19549.86chloride channel B [more]
AT5G40890.19.7e-19249.37chloride channel A [more]
AT5G40890.27.0e-16650.58chloride channel A [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002251Chloride channel ClC-plantPRINTSPR01120CLCHANNELPLTcoord: 75..83
score: 40.74
coord: 300..310
score: 81.82
coord: 286..294
score: 77.78
coord: 318..325
score: 72.22
IPR001807Chloride channel, voltage gatedPRINTSPR00762CLCHANNELcoord: 560..574
score: 38.67
coord: 170..187
score: 68.0
coord: 504..520
score: 50.71
coord: 472..492
score: 57.05
coord: 522..541
score: 48.1
coord: 201..220
score: 43.7
coord: 265..284
score: 67.9
IPR001807Chloride channel, voltage gatedPFAMPF00654Voltage_CLCcoord: 157..554
e-value: 5.3E-81
score: 272.6
NoneNo IPR availableGENE3D1.10.3080.10Clc chloride channelcoord: 93..574
e-value: 3.3E-109
score: 367.7
NoneNo IPR availablePANTHERPTHR11689:SF147CHLORIDE CHANNEL PROTEINcoord: 30..728
NoneNo IPR availablePANTHERPTHR11689CHLORIDE CHANNEL PROTEIN CLC FAMILY MEMBERcoord: 30..728
NoneNo IPR availableCDDcd04591CBS_pair_voltage-gated_CLC_euk_baccoord: 585..727
e-value: 9.89972E-18
score: 77.5597
NoneNo IPR availableSUPERFAMILY54631CBS-domain paircoord: 576..731
IPR014743Chloride channel, coreSUPERFAMILY81340Clc chloride channelcoord: 79..575

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr030558.1Sgr030558.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902476 chloride transmembrane transport
biological_process GO:0006821 chloride transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009705 plant-type vacuole membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015108 chloride transmembrane transporter activity
molecular_function GO:0005247 voltage-gated chloride channel activity