Sgr030505 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr030505
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	Glucan endo-1,3-beta-D-glucosidase
Location	tig00154107: 1061454 .. 1063602 (+)
RNA-Seq Expression	Sgr030505
Synteny	Sgr030505

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCAAAATCTGGATTACAAGACCAAAACGAGTGTATATTCACAGCATTGCTTTTGTTGTTCTTGACGATTGTGTCGAATGGGGCGAGTGTTGGAGTGAACTGGGGAACCATGGCGACTCACCAGCTCCCACCGGAGAAGGTGGTTAACATGCTGCAAGATAATGGGTTTCATAAACTGAAGCTTTTCGAAGCTGAGGAGAGGATTTTGGAAGCCCTGATTGGGACTGATATTGAGGTTATGCTGGCCATTCCAAATTCCATGTTGCAGATGATGAGTGAAGATCCTGAGGCTGCAGCTTCTTGGGTGGATGCCAATGTCACCACTTACTCTTATCATGGTGGTATCAATATCAAGTTGAGTATTCAAGATTCTTGATCTCATTAACTTTGCATTTGCCTTTGCCTTTGCCTTTTGCTCATCATTTTGTGTTCTTCAAGTGTTATATGATTTTCCCACTTCATGGTTTCTGTTTTGAATATTTTCTTTTCAAGCTGCTGCTGCGTTTTCCATTTCTCTCAGAGAATTTATTCTTTCAATTTATGGACTACCGATCTGTTTCTTTCTTTGTTTAGTTCTCTACATTTGTCTCTTTTACTGAAATATTTTTTCTATAAACATTTTTGAAATAAACAGCAGTACTTTTGACTATGTAACTAAATGCTTCTTCTTAAAGTCATGTCAAACTCGCTCTTAGTTTGACTGATCACCTCAGGACCAATGATTATAGGTCTTTTTTCCATAACTACTAGATTAAATACAAATAGTTTCTCTCTTTTATTTTGCCAATTAGATTCCAAAACCATGAGGTAGTAACAAAATGCCACTCCTAAATGATCGCATTCATTGGACCACTCTTTGGTGCTGCAACCTAGTTTAGTGGCTGAAAGAGTTAAGATTTGTTAATGCATTTTTAGAAGTTCAAATATCAATTTTATGAATGTTAAATTAGCTTAACAAGCATATGTTCTTTCTCTCTCTCTCTCTCCCATAAAATTCCCTTGAAAACTGATCTTGCAGGTATGTTGCTGTAGGCAATGAACCCTTTCTTAAGTCCTACAATGGCACCTATCTTCAGTTCACATTGCCAGCCCTGAGGAATATCCAGCAAGCGTTAAATGATGCAGGGCTTGGATCAAAGGTCAAAGCTACTATTCCTTTCAATGCTGATATTTACAACTCCCCGGACTCAAACCCGGTTCCATCTGCAGGCGACTTCCGGTCCGACGTCCGAGATTTGACGATTGAGATTCTACACTTCCTATCCATAAGCAATGCACCTTTCACAGTCAATATCTACCCCTTCCTTTCCCTCTACAGCAATGACTACTTCCCAATGGACTTTGCGTTCTTCGATGGAACATACAAGCCCATCAGAGATGGAGATCTAACATACACTAACGTGTTCGATGCAAATTTCGACACTCTCGTTTCGGCTCTAACGAAAGCAGGATACCCTGACATGAAGATCATAGTAGGGGAAGTCGGGTGGCCAACCGACGGCGACAAACACGCCAATATCCAAAATGCAAAACGGTTCAACCAGGGATTGCTGAGACATGCTCTAAGCGGAGAGGGAACCCCGGCAAGGATAGGCATAATAGACATTTATCTCTTCAGCCTGATTGATGAAAATGCCAAAAGCATAGAACCAGGGAGCTTTGAGAGGCATTGGGGGATATTTGAGTTTGATGGTAAGCCAAAATATGAACTAGATCTCTCTGGAACAGAAGAAGAAAAGGGCTTGGTAGCAGTAGAAGGAGTGAGATATATGCTTAAAAGATGGTGTGTTTTGAACCCAAATGTGAATGATTGGGAAGGCTTGGCTGATAGTGTAGATTATGCTTGTAGCCTATCAGATTGCACTGCATTGGAATATGGGTCTTCTTGCAATCAACTAACTGCCCAAGGGAATGCTTCTTATGCCTTCAATATGTACTATCAAGTCAACAGCCAGAAGAGTTGGACCTGTGATTTCTCTGGTTTGGCTGTGGTGACTGACGAGGATCCATCTAATGGGAACTGCCAGTTCCCAGTTATGATTGATTATACTTCTTCTTCTTCTTCTCCATTTCTGCTGCATGAAAGATTTTTAGATCTTCTGGTTGCAGTCTTCATGGGATTAGTGGTGTTTTTGTTTCTGGAATGA

mRNA sequence

ATGTCAAAATCTGGATTACAAGACCAAAACGAGTGTATATTCACAGCATTGCTTTTGTTGTTCTTGACGATTGTGTCGAATGGGGCGAGTGTTGGAGTGAACTGGGGAACCATGGCGACTCACCAGCTCCCACCGGAGAAGGTGGTTAACATGCTGCAAGATAATGGGTTTCATAAACTGAAGCTTTTCGAAGCTGAGGAGAGGATTTTGGAAGCCCTGATTGGGACTGATATTGAGGTTATGCTGGCCATTCCAAATTCCATGTTGCAGATGATGAGTGAAGATCCTGAGGCTGCAGCTTCTTGGGTGGATGCCAATGTCACCACTTACTCTTATCATGGTGGTATCAATATCAAGTTGAGCAATGAACCCTTTCTTAAGTCCTACAATGGCACCTATCTTCAGTTCACATTGCCAGCCCTGAGGAATATCCAGCAAGCGTTAAATGATGCAGGGCTTGGATCAAAGGTCAAAGCTACTATTCCTTTCAATGCTGATATTTACAACTCCCCGGACTCAAACCCGGTTCCATCTGCAGGCGACTTCCGGTCCGACGTCCGAGATTTGACGATTGAGATTCTACACTTCCTATCCATAAGCAATGCACCTTTCACAGTCAATATCTACCCCTTCCTTTCCCTCTACAGCAATGACTACTTCCCAATGGACTTTGCGTTCTTCGATGGAACATACAAGCCCATCAGAGATGGAGATCTAACATACACTAACGTGTTCGATGCAAATTTCGACACTCTCGTTTCGGCTCTAACGAAAGCAGGATACCCTGACATGAAGATCATAGTAGGGGAAGTCGGGTGGCCAACCGACGGCGACAAACACGCCAATATCCAAAATGCAAAACGGTTCAACCAGGGATTGCTGAGACATGCTCTAAGCGGAGAGGGAACCCCGGCAAGGATAGGCATAATAGACATTTATCTCTTCAGCCTGATTGATGAAAATGCCAAAAGCATAGAACCAGGGAGCTTTGAGAGGCATTGGGGGATATTTGAGTTTGATGGTAAGCCAAAATATGAACTAGATCTCTCTGGAACAGAAGAAGAAAAGGGCTTGGTAGCAGTAGAAGGAGTGAGATATATGCTTAAAAGATGGTGTGTTTTGAACCCAAATGTGAATGATTGGGAAGGCTTGGCTGATAGTGTAGATTATGCTTGTAGCCTATCAGATTGCACTGCATTGGAATATGGGTCTTCTTGCAATCAACTAACTGCCCAAGGGAATGCTTCTTATGCCTTCAATATGTACTATCAAGTCAACAGCCAGAAGAGTTGGACCTGTGATTTCTCTGGTTTGGCTGTGGTGACTGACGAGGATCCATCTAATGGGAACTGCCAGTTCCCAGTTATGATTGATTATACTTCTTCTTCTTCTTCTCCATTTCTGCTGCATGAAAGATTTTTAGATCTTCTGGTTGCAGTCTTCATGGGATTAGTGGTGTTTTTGTTTCTGGAATGA

Coding sequence (CDS)

ATGTCAAAATCTGGATTACAAGACCAAAACGAGTGTATATTCACAGCATTGCTTTTGTTGTTCTTGACGATTGTGTCGAATGGGGCGAGTGTTGGAGTGAACTGGGGAACCATGGCGACTCACCAGCTCCCACCGGAGAAGGTGGTTAACATGCTGCAAGATAATGGGTTTCATAAACTGAAGCTTTTCGAAGCTGAGGAGAGGATTTTGGAAGCCCTGATTGGGACTGATATTGAGGTTATGCTGGCCATTCCAAATTCCATGTTGCAGATGATGAGTGAAGATCCTGAGGCTGCAGCTTCTTGGGTGGATGCCAATGTCACCACTTACTCTTATCATGGTGGTATCAATATCAAGTTGAGCAATGAACCCTTTCTTAAGTCCTACAATGGCACCTATCTTCAGTTCACATTGCCAGCCCTGAGGAATATCCAGCAAGCGTTAAATGATGCAGGGCTTGGATCAAAGGTCAAAGCTACTATTCCTTTCAATGCTGATATTTACAACTCCCCGGACTCAAACCCGGTTCCATCTGCAGGCGACTTCCGGTCCGACGTCCGAGATTTGACGATTGAGATTCTACACTTCCTATCCATAAGCAATGCACCTTTCACAGTCAATATCTACCCCTTCCTTTCCCTCTACAGCAATGACTACTTCCCAATGGACTTTGCGTTCTTCGATGGAACATACAAGCCCATCAGAGATGGAGATCTAACATACACTAACGTGTTCGATGCAAATTTCGACACTCTCGTTTCGGCTCTAACGAAAGCAGGATACCCTGACATGAAGATCATAGTAGGGGAAGTCGGGTGGCCAACCGACGGCGACAAACACGCCAATATCCAAAATGCAAAACGGTTCAACCAGGGATTGCTGAGACATGCTCTAAGCGGAGAGGGAACCCCGGCAAGGATAGGCATAATAGACATTTATCTCTTCAGCCTGATTGATGAAAATGCCAAAAGCATAGAACCAGGGAGCTTTGAGAGGCATTGGGGGATATTTGAGTTTGATGGTAAGCCAAAATATGAACTAGATCTCTCTGGAACAGAAGAAGAAAAGGGCTTGGTAGCAGTAGAAGGAGTGAGATATATGCTTAAAAGATGGTGTGTTTTGAACCCAAATGTGAATGATTGGGAAGGCTTGGCTGATAGTGTAGATTATGCTTGTAGCCTATCAGATTGCACTGCATTGGAATATGGGTCTTCTTGCAATCAACTAACTGCCCAAGGGAATGCTTCTTATGCCTTCAATATGTACTATCAAGTCAACAGCCAGAAGAGTTGGACCTGTGATTTCTCTGGTTTGGCTGTGGTGACTGACGAGGATCCATCTAATGGGAACTGCCAGTTCCCAGTTATGATTGATTATACTTCTTCTTCTTCTTCTCCATTTCTGCTGCATGAAAGATTTTTAGATCTTCTGGTTGCAGTCTTCATGGGATTAGTGGTGTTTTTGTTTCTGGAATGA

Protein sequence

MSKSGLQDQNECIFTALLLLFLTIVSNGASVGVNWGTMATHQLPPEKVVNMLQDNGFHKLKLFEAEERILEALIGTDIEVMLAIPNSMLQMMSEDPEAAASWVDANVTTYSYHGGINIKLSNEPFLKSYNGTYLQFTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRSDVRDLTIEILHFLSISNAPFTVNIYPFLSLYSNDYFPMDFAFFDGTYKPIRDGDLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARIGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLVAVEGVRYMLKRWCVLNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASYAFNMYYQVNSQKSWTCDFSGLAVVTDEDPSNGNCQFPVMIDYTSSSSSPFLLHERFLDLLVAVFMGLVVFLFLE

Homology

[Close]

BLAST of Sgr030505 vs. NCBI nr
Match: XP_022157557.1 (glucan endo-1,3-beta-glucosidase 8 [Momordica charantia])

HSP 1 Score: 918.3 bits (2372), Expect = 2.8e-263
Identity = 447/493 (90.67%), Postives = 471/493 (95.54%), Query Frame = 0


Query: 1   MSKSGLQDQNECIFTALLLLFLTIVSNGASVGVNWGTMATHQLPPEKVVNMLQDNGFHKL 60
           MSKSGLQ   +CIFTAL+LL+ TI  NG SVGVNWGTMATHQLPPEKVV MLQ+NGF KL
Sbjct: 1   MSKSGLQIHRDCIFTALILLYSTIFPNGESVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60

Query: 61  KLFEAEERILEALIGTDIEVMLAIPNSMLQMMSEDPEAAASWVDANVTTYSYHGGINIK- 120
           KLFEAE+RILEALIG+DIEVMLAIPNSMLQ+MSEDPEAAASWVD+NVTTYSYHGG+NIK 
Sbjct: 61  KLFEAEDRILEALIGSDIEVMLAIPNSMLQIMSEDPEAAASWVDSNVTTYSYHGGVNIKY 120

Query: 121 --LSNEPFLKSYNGTYLQFTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180
             + NEPFLKSYNGTYLQFTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP
Sbjct: 121 VAVGNEPFLKSYNGTYLQFTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180

Query: 181 SAGDFRSDVRDLTIEILHFLSISNAPFTVNIYPFLSLYSNDYFPMDFAFFDGTYKPIRDG 240
           SAG+FRSDVRDLTIEILHFLS +NAPFTVNIYPFLSLYSNDYFP+DFAFFDGTYKPIRDG
Sbjct: 181 SAGEFRSDVRDLTIEILHFLSTNNAPFTVNIYPFLSLYSNDYFPLDFAFFDGTYKPIRDG 240

Query: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHA 300
           DL YTNVFDANFDTLVSALTKAGYP+MKIIVGEVGWPTDGDKHANIQ AKRFNQGLLRHA
Sbjct: 241 DLAYTNVFDANFDTLVSALTKAGYPEMKIIVGEVGWPTDGDKHANIQYAKRFNQGLLRHA 300

Query: 301 LSGEGTPARIGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360
           LSGEGTPAR+GIIDIYLFSLIDENAKSIEPGSFERHWG+FEFDGKPKYELDLSGTE+EKG
Sbjct: 301 LSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWGLFEFDGKPKYELDLSGTEQEKG 360

Query: 361 LVAVEGVRYMLKRWCVLNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASY 420
           LVAVEGVRYM+KRWCVLNPN+NDWEGLAD+VDYACSLSDCTALEYGSSCNQLTAQGNASY
Sbjct: 361 LVAVEGVRYMVKRWCVLNPNLNDWEGLADNVDYACSLSDCTALEYGSSCNQLTAQGNASY 420

Query: 421 AFNMYYQVNSQKSWTCDFSGLAVVTDEDPSNGNCQFPVMIDYTSSSSSPFLLHERFLDLL 480
           AFNMYYQVNSQKSW CDFSGLAVVT+EDPS GNCQFPVMIDYTSSSSSPFL+ ERFLDLL
Sbjct: 421 AFNMYYQVNSQKSWNCDFSGLAVVTEEDPSYGNCQFPVMIDYTSSSSSPFLVDERFLDLL 480

Query: 481 VAVFMGLVVFLFL 491
           VAV MGL+V  F+
Sbjct: 481 VAVIMGLLVSFFV 493

[Close]

BLAST of Sgr030505 vs. NCBI nr
Match: XP_038890697.1 (glucan endo-1,3-beta-glucosidase 8-like [Benincasa hispida])

HSP 1 Score: 892.9 bits (2306), Expect = 1.3e-255
Identity = 436/497 (87.73%), Postives = 460/497 (92.56%), Query Frame = 0


Query: 1   MSKSGLQ-DQNECIF--TALLLLFLTIVSNGASVGVNWGTMATHQLPPEKVVNMLQDNGF 60
           MSKSGLQ  +N CIF    L+ L L I  +G SVGVNWGTMATHQLPPEKVV MLQ+NGF
Sbjct: 1   MSKSGLQFHRNYCIFRIRTLVFLLLMIFPDGLSVGVNWGTMATHQLPPEKVVKMLQENGF 60

Query: 61  HKLKLFEAEERILEALIGTDIEVMLAIPNSMLQMMSEDPEAAASWVDANVTTYSYHGGIN 120
           HKLKLFEAE+RILEALIG+DIEVMLAIPNSMLQ+MS++P AAASWVD+NVT Y+YHGG+ 
Sbjct: 61  HKLKLFEAEDRILEALIGSDIEVMLAIPNSMLQLMSQNPRAAASWVDSNVTAYTYHGGVK 120

Query: 121 IK---LSNEPFLKSYNGTYLQFTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSN 180
           IK   + NEPFLKSYNGTYLQ TLPALRNIQ ALNDAGLGSKVKATIPFNADIYNSPDSN
Sbjct: 121 IKYVAVGNEPFLKSYNGTYLQLTLPALRNIQAALNDAGLGSKVKATIPFNADIYNSPDSN 180

Query: 181 PVPSAGDFRSDVRDLTIEILHFLSISNAPFTVNIYPFLSLYSNDYFPMDFAFFDGTYKPI 240
           PVPSAG+FR DVRDLTIEILHFLSI+NAPFTVNIYPFLSLY NDYFPMDFAFFDGTYKPI
Sbjct: 181 PVPSAGEFRPDVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPMDFAFFDGTYKPI 240

Query: 241 RDGDLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLL 300
           +DGDLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLL
Sbjct: 241 KDGDLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLL 300

Query: 301 RHALSGEGTPARIGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEE 360
           RHALSGEGTPAR+GIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEE
Sbjct: 301 RHALSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEE 360

Query: 361 EKGLVAVEGVRYMLKRWCVLNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGN 420
           EKGL+ VEGVRYM+KRWC+LNPNVNDWEGLADS+DYACSLSDCTALEYGSSCNQLTAQGN
Sbjct: 361 EKGLIPVEGVRYMVKRWCILNPNVNDWEGLADSIDYACSLSDCTALEYGSSCNQLTAQGN 420

Query: 421 ASYAFNMYYQVNSQKSWTCDFSGLAVVTDEDPSNGNCQFPVMIDYTSSSSSPFLLHERFL 480
           ASYAFNMYYQVNSQKSW CDF GLAVVT +DPS GNCQFP+MIDY SSS SPFL  ERFL
Sbjct: 421 ASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQFPIMIDYASSSYSPFLFDERFL 480

Query: 481 DLLVAVFMGLVVFLFLE 492
           DLLV VFMG +VFL LE
Sbjct: 481 DLLVEVFMGFMVFLILE 497

[Close]

BLAST of Sgr030505 vs. NCBI nr
Match: XP_022965692.1 (glucan endo-1,3-beta-glucosidase 8-like [Cucurbita maxima])

HSP 1 Score: 872.1 bits (2252), Expect = 2.3e-249
Identity = 422/494 (85.43%), Postives = 457/494 (92.51%), Query Frame = 0


Query: 1   MSKSGLQDQNECIFTALLLLFLTIVSNGASVGVNWGTMATHQLPPEKVVNMLQDNGFHKL 60
           MSKSGLQ      F  +LLLFLTI+ NG +VGVNWGTMATHQLPPEKVV MLQ+NGF KL
Sbjct: 1   MSKSGLQIHRNFTFRTILLLFLTIIPNGETVGVNWGTMATHQLPPEKVVKMLQENGFLKL 60

Query: 61  KLFEAEERILEALIGTDIEVMLAIPNSMLQMMSEDPEAAASWVDANVTTYSYHGGINIK- 120
           KLFEAE+RILEALIGTDIEVMLA+PNSMLQ+MS+D +AAASWVDANVT Y+YHGG+NIK 
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSDDSKAAASWVDANVTAYTYHGGVNIKY 120

Query: 121 --LSNEPFLKSYNGTYLQFTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180
             + NEPFLKSYNGTYLQ TLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP
Sbjct: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180

Query: 181 SAGDFRSDVRDLTIEILHFLSISNAPFTVNIYPFLSLYSNDYFPMDFAFFDGTYKPIRDG 240
           SAGDFR +VRDLTIEILHFLSI+NAPFTVNIYPFLSLY NDYFP+DFAFF GTYKPI+DG
Sbjct: 181 SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFGGTYKPIKDG 240

Query: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHA 300
           DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGE+GWPTDGDKHANIQ+AKRFNQGLLRHA
Sbjct: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA 300

Query: 301 LSGEGTPARIGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360
           LSGEGTPAR+G+ID+YLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTE+EKG
Sbjct: 301 LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEQEKG 360

Query: 361 LVAVEGVRYMLKRWCVLNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASY 420
           L+AVEGV+YM K+WCVLNPNV DWEGLAD++DYACSLSDCTALEYGSSCNQLTA+GNASY
Sbjct: 361 LIAVEGVKYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY 420

Query: 421 AFNMYYQVNSQKSWTCDFSGLAVVTDEDPSNGNCQFPVMIDYTSSSSSPFLLHERFLDLL 480
           AFNMYYQ+NSQK W C+FSGLA+VT++DPS GNCQFP+MIDY SSSSS       FLDLL
Sbjct: 421 AFNMYYQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSS-------FLDLL 480

Query: 481 VAVFMGLVVFLFLE 492
           VA+FMG VVFL LE
Sbjct: 481 VAIFMGFVVFLCLE 487

[Close]

BLAST of Sgr030505 vs. NCBI nr
Match: KAG7021271.1 (Glucan endo-1,3-beta-glucosidase 8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 869.8 bits (2246), Expect = 1.1e-248
Identity = 423/494 (85.63%), Postives = 455/494 (92.11%), Query Frame = 0


Query: 1   MSKSGLQDQNECIFTALLLLFLTIVSNGASVGVNWGTMATHQLPPEKVVNMLQDNGFHKL 60
           MSK  LQ     I   +LLLFLTI+ NG SVGVNWGTMATHQLPPEKVV MLQ+NGF KL
Sbjct: 1   MSKFELQIHRNFILRTILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKL 60

Query: 61  KLFEAEERILEALIGTDIEVMLAIPNSMLQMMSEDPEAAASWVDANVTTYSYHGGINIK- 120
           KLFEAE+RILEALIGTDIEVMLA+PNSMLQ+MSED +AAA WVDANVT Y+YHGG+NIK 
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSEDSKAAAYWVDANVTAYTYHGGVNIKY 120

Query: 121 --LSNEPFLKSYNGTYLQFTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180
             + NEPFLKSYNGTYLQ TLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP
Sbjct: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180

Query: 181 SAGDFRSDVRDLTIEILHFLSISNAPFTVNIYPFLSLYSNDYFPMDFAFFDGTYKPIRDG 240
           SAGDFR +VRDLTIEILHFLSI+NAPFTVNIYPFLSLY NDYFP+DFAFFDGTYKPI+DG
Sbjct: 181 SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDG 240

Query: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHA 300
           DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGE+GWPTDGDKHANIQ+AKRFNQGLLRHA
Sbjct: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA 300

Query: 301 LSGEGTPARIGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360
           LSGEGTPAR+G+ID+YLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG
Sbjct: 301 LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360

Query: 361 LVAVEGVRYMLKRWCVLNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASY 420
           L+AVEGVRYM K+WCVLNPNV DWEGLAD++DYACSLSDCTALEYGSSCNQLTA+GNASY
Sbjct: 361 LIAVEGVRYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY 420

Query: 421 AFNMYYQVNSQKSWTCDFSGLAVVTDEDPSNGNCQFPVMIDYTSSSSSPFLLHERFLDLL 480
           AFNMY+Q+NSQK W C+FSGLA+VT++DPS GNCQFP+MIDY SSSSS       FLDLL
Sbjct: 421 AFNMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSS-------FLDLL 480

Query: 481 VAVFMGLVVFLFLE 492
           VA+FMG VVFL LE
Sbjct: 481 VAIFMGFVVFLSLE 487

[Close]

BLAST of Sgr030505 vs. NCBI nr
Match: XP_022946334.1 (glucan endo-1,3-beta-glucosidase 8-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 869.0 bits (2244), Expect = 1.9e-248
Identity = 427/494 (86.44%), Postives = 452/494 (91.50%), Query Frame = 0


Query: 1   MSKSGLQDQNECIFTALLLLFLTIVSNGASVGVNWGTMATHQLPPEKVVNMLQDNGFHKL 60
           MS+S L+     IF AL+LL LT+V NG SVGVNWGTMATHQLPPEKVV MLQ+NGF KL
Sbjct: 1   MSESVLRFHRNWIFIALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60

Query: 61  KLFEAEERILEALIGTDIEVMLAIPNSMLQMMSEDPEAAASWVDANVTTYSYHGGINIK- 120
           KLFEAE+RILEALIGTDIEVMLAIPNSMLQ+MSEDPEAAA WVD+NVT Y+YHGG+ IK 
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY 120

Query: 121 --LSNEPFLKSYNGTYLQFTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180
             + NEPFLKSYNG YLQ TLPALRNIQQALNDAGLGSKVKATIPFNAD YNSPDSNPVP
Sbjct: 121 VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSKVKATIPFNADTYNSPDSNPVP 180

Query: 181 SAGDFRSDVRDLTIEILHFLSISNAPFTVNIYPFLSLYSNDYFPMDFAFFDGTYKPIRDG 240
           SAGDFR DVRDLTIEILHFLSI+NAPFTVNIYPFLSLY NDYFP+DFAFFDGTYK I+DG
Sbjct: 181 SAGDFRPDVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKSIKDG 240

Query: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHA 300
           DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHA
Sbjct: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHA 300

Query: 301 LSGEGTPARIGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360
           LSGEGTPAR+GIIDIYLFSLIDENAKSIEPGSFERHWGIF+FDGKPKYELDLSG E +KG
Sbjct: 301 LSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFKFDGKPKYELDLSGIEAQKG 360

Query: 361 LVAVEGVRYMLKRWCVLNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASY 420
           L+AVEGVRYM+KRWCVLNPNV DWEGLA SVDYACSLSDCTALEYGSSCNQL+A+GNASY
Sbjct: 361 LIAVEGVRYMVKRWCVLNPNVKDWEGLAYSVDYACSLSDCTALEYGSSCNQLSAEGNASY 420

Query: 421 AFNMYYQVNSQKSWTCDFSGLAVVTDEDPSNGNCQFPVMIDYTSSSSSPFLLHERFLDLL 480
           AFNMYYQ+NSQKSW CDFSG+AVVT EDPS G+CQFPVMIDYT SSSS       FLDLL
Sbjct: 421 AFNMYYQMNSQKSWNCDFSGMAVVTQEDPSYGSCQFPVMIDYTFSSSSSSSSSSSFLDLL 480

Query: 481 VAVFMGLVVFLFLE 492
           VA FMG +VFLFLE
Sbjct: 481 VAGFMGFLVFLFLE 494

[Close]

BLAST of Sgr030505 vs. ExPASy Swiss-Prot
Match: Q6NKW9 (Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 PE=2 SV=2)

HSP 1 Score: 527.7 bits (1358), Expect = 1.4e-148
Identity = 260/464 (56.03%), Postives = 333/464 (71.77%), Query Frame = 0


Query: 22  LTIVSNGASVGVNWGTMATHQLPPEKVVNMLQDNGFHKLKLFEAEERILEALIGTDIEVM 81
           L I+ NG  +GVNWGTMATH+LPP+ VV ML+DN  +K+KLF+A+E  + AL G+ +EVM
Sbjct: 18  LGILVNG--LGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGSGLEVM 77

Query: 82  LAIPNSMLQMMSEDPEAAASWVDANVTTYSYHGGINI---KLSNEPFLKSYNGTYLQFTL 141
           +AIPN  L++M+   + A  WV  NVT Y++ GG+NI    + NEPFLKSYNG+++  T 
Sbjct: 78  VAIPNDQLKVMT-SYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTF 137

Query: 142 PALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRSDVRDLTIEILHFLS 201
           PAL NIQ ALN+AGLG+ VKAT+P NAD+Y+SP SNPVPSAG FR D+     +I+ FL 
Sbjct: 138 PALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLG 197

Query: 202 ISNAPFTVNIYPFLSLYSNDYFPMDFAFFDGTYKPIRDGDLTYTNVFDANFDTLVSALTK 261
            +NAP T+NIYPFLSLY ND FP+++AFFDG  +PI D  + YTNVFDANFDTLVS+L  
Sbjct: 198 KNNAPITINIYPFLSLYGNDDFPLNYAFFDGA-EPINDNGIDYTNVFDANFDTLVSSLKA 257

Query: 262 AGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARIGIIDIYLFSLI 321
            G+ DM IIVGEVGWPT+GDKHAN  +A RF  GLL    + +GTP R   I++YLF L+
Sbjct: 258 VGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEVYLFGLL 317

Query: 322 DENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLVAVEGVRYMLKRWCVLNPNV 381
           DE+AKSI PG FERHWGIF+FDG+PK+ +DLSG  + K L+  + V Y+  +WC  NP  
Sbjct: 318 DEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEA 377

Query: 382 NDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASYAFNMYYQVNSQKSWTCDFSGL 441
            D   LA ++DYAC+ SDCTAL YGSSCN L A GNASYAFNM++QV +Q    C F GL
Sbjct: 378 KDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESACYFQGL 437

Query: 442 AVVTDEDPSNGNCQFPVMIDYTSSSSSPFLLHERFLDLLVAVFM 483
           A +T ++ S G C FP+ I   +SS+S F      L +L+  F+
Sbjct: 438 ATITTQNISQGQCNFPIQI--VASSASSFSCSSYSLVVLIVWFL 475

[Close]

BLAST of Sgr030505 vs. ExPASy Swiss-Prot
Match: Q93Z08 (Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 PE=2 SV=2)

HSP 1 Score: 488.8 bits (1257), Expect = 7.2e-137
Identity = 246/476 (51.68%), Postives = 322/476 (67.65%), Query Frame = 0


Query: 17  LLLLFLTIVSNGASVGVNWGTMATHQLPPEKVVNMLQDNGFHKLKLFEAEERILEALIGT 76
           LLL    +    +S+G NWGT A+H LPP+ VV ML++NG  K+KLF+AE   L AL  +
Sbjct: 8   LLLAVALLCQRASSIGANWGTQASHPLPPDIVVRMLRENGIQKVKLFDAEYDTLRALGKS 67

Query: 77  DIEVMLAIPNSMLQMMSEDPEAAASWVDANVTTYSYHGGINIK---LSNEPFLKSYNGTY 136
            IEVM+ IPN ML  ++   +AA  WV  NV+T+     +NI+   + NEPFL +YNG+Y
Sbjct: 68  GIEVMVGIPNEMLATLASSLKAAEKWVAKNVSTHISTDNVNIRYVAVGNEPFLSTYNGSY 127

Query: 137 LQFTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRSDVRDLTIEI 196
           L  T PALRNIQ A+  AGL ++VK T P NAD+Y+S  S   PS GDFR+++RDL I I
Sbjct: 128 LSTTFPALRNIQIAIIKAGLQNQVKVTCPLNADVYDS--STTFPSGGDFRANIRDLMITI 187

Query: 197 LHFLSISNAPFTVNIYPFLSLYSNDYFPMDFAFFDGTYKPIRDGDLTYTNVFDANFDTLV 256
           + FLS +  PFTVNIYP++SLY+N  FP+D+AFFDG  +P+ DG   Y N+FDAN+DTLV
Sbjct: 188 VKFLSENGGPFTVNIYPYISLYTNPDFPVDYAFFDGNAQPLNDGGTFYYNMFDANYDTLV 247

Query: 257 SALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARIGIIDIY 316
            AL K G+ +M II+GE+GWPTDGD +AN+  AK+FNQG + H   G+GTP R G ID Y
Sbjct: 248 HALEKNGFGNMPIIIGEIGWPTDGDSNANLDYAKKFNQGFMAHISGGKGTPRRPGPIDAY 307

Query: 317 LFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLVAVEGVRYMLKRWCV 376
           LFSLIDE+AKS++PG FERHWGIF FDG PKY L+L GT     L+  +GVRY+ ++WCV
Sbjct: 308 LFSLIDEDAKSVQPGYFERHWGIFTFDGLPKYALNL-GTTNTGALIQAKGVRYLERKWCV 367

Query: 377 LNPNVN-DWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASYAFNMYYQVNSQKSWT 436
           + PNV  D   +A +V YACSL DCT+L  G+SC  L  + N SYAFN YYQ+  Q    
Sbjct: 368 MKPNVRLDDPQVAPAVSYACSLGDCTSLGVGTSCANLDGKQNISYAFNSYYQIQDQLDTA 427

Query: 437 CDFSGLAVVTDEDPSNGNCQFPVMID-YTSSSSSPFLLHERFLDLLVAVFMGLVVF 488
           C F  ++ VT  DPS G C+FP+MI+ Y   ++     H  F  LL+   + + +F
Sbjct: 428 CKFPNISEVTKTDPSTGTCRFPIMIEPYYGGAARE---HGFFFPLLMVAAIAVSIF 477

[Close]

BLAST of Sgr030505 vs. ExPASy Swiss-Prot
Match: Q9M088 (Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 PE=2 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 4.8e-133
Identity = 231/461 (50.11%), Postives = 317/461 (68.76%), Query Frame = 0


Query: 13  IFTALLLLFLTIVSNGASVGVNWGTMATHQLPPEKVVNMLQDNGFHKLKLFEAEERILEA 72
           +F  + LL+ +++     +GVNWG+ A H LPP  VV +L++NG  K+KLFEA+  IL+A
Sbjct: 9   VFFVITLLYASLLIEVEGIGVNWGSQARHPLPPATVVRLLRENGIQKVKLFEADSAILKA 68

Query: 73  LIGTDIEVMLAIPNSMLQMMSEDPEAAASWVDANVTTYSYHGGINIK---LSNEPFLKSY 132
           L  T I+VM+ IPN +L  ++    AA  WV  NV+ +    G++I+   + NEPFLK++
Sbjct: 69  LSRTGIQVMVGIPNDLLAPLAGSVAAAERWVSQNVSAHVSSNGVDIRYVAVGNEPFLKAF 128

Query: 133 NGTYLQFTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRSDVRDL 192
           NGT+   TLPAL+NIQ A+  AGL ++VK T+P NAD+Y S  ++ +PS GDFR ++RDL
Sbjct: 129 NGTFEGITLPALQNIQSAIIKAGLATQVKVTVPLNADVYQS--ASNLPSDGDFRPEIRDL 188

Query: 193 TIEILHFLSISNAPFTVNIYPFLSLYSNDYFPMDFAFFDGTYKPIRDGDLTYTNVFDANF 252
            + I+ FLS + APFT+NIYPF+SLY++  FP++FAFFDGT  PI D    Y NV DAN+
Sbjct: 189 MLNIVKFLSDNGAPFTINIYPFISLYNDPNFPVEFAFFDGTGTPINDNGRIYDNVLDANY 248

Query: 253 DTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARIGI 312
           DTLV +L K G+ ++ IIVGEVGWPTDGDK+AN+  A+R+NQG +    + +GTP R G 
Sbjct: 249 DTLVWSLQKNGFGNLTIIVGEVGWPTDGDKNANLMYARRYNQGFMNRQKANKGTPMRPGA 308

Query: 313 IDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLVAVEGVRYMLK 372
           +D YLF LIDE+AKSI+PG+FERHWGIF  DG+PKY+L L       GL+  + V Y+ K
Sbjct: 309 MDAYLFGLIDEDAKSIQPGNFERHWGIFYIDGQPKYQLSLG---SGNGLIPAKDVHYLAK 368

Query: 373 RWCVLNPNVNDWE-GLADSVDYACSLSDCTALEYGSSCNQLTAQGNASYAFNMYYQVNSQ 432
           +WC+L PN N  +  L  SV YAC  +DCT+L YGSSC  L    N SYAFN YYQV++Q
Sbjct: 369 KWCILAPNANLQDPQLGPSVSYACDHADCTSLGYGSSCGNLNLAQNVSYAFNSYYQVSNQ 428

Query: 433 KSWTCDFSGLAVVTDEDPSNGNCQFPVMIDYTSSSSSPFLL 470
               C F GL++V+  DPS G+C+F +MI    +S +  ++
Sbjct: 429 LDSACKFPGLSIVSTRDPSVGSCKFKIMIKSEDASEASAMM 464

[Close]

BLAST of Sgr030505 vs. ExPASy Swiss-Prot
Match: Q9FGH4 (Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 PE=2 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 8.9e-95
Identity = 190/480 (39.58%), Postives = 285/480 (59.38%), Query Frame = 0


Query: 18  LLLFLTIVSNGAS--------VGVNWGTMATHQLPPEKVVNMLQDNGFHKLKLFEAEERI 77
           L L L  V+ G S        VG+NWGT A+H LPP KVV +L+ NG  K+KLF+A+ ++
Sbjct: 5   LFLLLLAVTAGLSLTGTTVRAVGINWGTEASHPLPPSKVVELLKSNGIVKVKLFDADPKV 64

Query: 78  LEALIGTDIEVMLAIPNSMLQMMSEDPEAAASWVDANVTTYSYHGGINIKL-----SNEP 137
           L AL G++I V + I NSML+ ++   + A SWV  NVT Y ++GG  +++       EP
Sbjct: 65  LRALSGSNIGVTIGIQNSMLKSLNASVKVAESWVHDNVTRY-FNGGNRVRIEYVAVGEEP 124

Query: 138 FLKSYNGTYLQFTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRS 197
           FL+SY   Y  F + A  NIQ AL  A L ++VK  +P + D + S      PS+G FR+
Sbjct: 125 FLQSYGNQYKPFVIGAAMNIQNALVKANLANEVKVVVPSSFDSFLSESGR--PSSGHFRA 184

Query: 198 DVRDLTIEILHFLSISNAPFTVNIYPFLSLYSNDYFPMDFAFFDGTYKPIRDGDLTYTNV 257
           D+    IE+L FL+  ++PF V I PFLS + N    +DF+ F  T K  +DG  TY N 
Sbjct: 185 DLNKTMIELLSFLTKHHSPFFVTISPFLSFHQNKNISLDFSLFKETAKAHKDGRKTYRNS 244

Query: 258 FDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTP 317
           FD ++DTLVSAL   G+ ++ I+V ++GWPTDG ++A    A+ F +GL+ H      + 
Sbjct: 245 FDLSYDTLVSALFTIGFSEVDIVVSKIGWPTDGAENATSLTAEAFFKGLIVHLEKKTASL 304

Query: 318 ARIGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLVAVEGV 377
            R   ++ Y+ SL+DE+ +++  G+FERHWG+F FDG+ KY    +     K  V  + V
Sbjct: 305 PR-PPVETYIESLLDEDQRNLSAGNFERHWGVFTFDGQAKYNFSFN----HKNQVNAQNV 364

Query: 378 RYMLKRWCVLNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASYAFNMYYQ 437
           +Y+  +WCV+N N +     A +++ AC+++DCT++  G SC+ +   GN SYAFN  YQ
Sbjct: 365 QYLPPKWCVVNNNKDLSNASARALE-ACAVADCTSILPGGSCSGIRWPGNVSYAFNSLYQ 424

Query: 438 VNSQKSWTCDFSGLAVVTDEDPSNGNCQFPVMIDYTSSSSSPFLLHERFLDLLVAVFMGL 485
            N   + +C+F GL ++T  DPS  NC+F + +D + SSS      + +  LL+ +  GL
Sbjct: 425 QNDHSAESCNFGGLGLITTVDPSEDNCRFSIQLDTSHSSSQTPNFFQSWPLLLLFLLSGL 475

[Close]

BLAST of Sgr030505 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.2e-59
Identity = 153/454 (33.70%), Postives = 238/454 (52.42%), Query Frame = 0


Query: 13  IFTALLLLFLTIVSNGAS-VGVNWGTMATHQLPPEKVVNMLQDNGFHKLKLFEAEERILE 72
           +F   LLL     +N  S +GVN+G ++ +    +  VN+L+     K++LF AE  +++
Sbjct: 11  LFLVFLLLQNFYSANSQSFLGVNYGQLSDNLPSLQATVNLLKSTTIQKVRLFGAEPAVIK 70

Query: 73  ALIGTDIEVMLAIPNSMLQMMSEDPEAAASWVDANVTT-YSYHGGINIKLSNEPFLKSYN 132
           A   T +E+++   N  +  ++ +P  A+ +V +NV + Y     I I + NE  L S +
Sbjct: 71  AFANTGVEIVIGFDNGDIPTLASNPNVASQFVKSNVMSFYPASNIIAITVGNE-VLTSGD 130

Query: 133 GTYLQFTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRSDVRDLT 192
              +   LPA++N+Q ALN A LG KVK +      + +   S P PS+G F   + D  
Sbjct: 131 QKLISQLLPAMQNVQNALNAASLGGKVKVSTVHAMAVLS--QSYP-PSSGVFNPGLGDTM 190

Query: 193 IEILHFLSISNAPFTVNIYPFLSLYSNDYFP--MDFAFFDGTYKPIRDGD-LTYTNVFDA 252
             +L F S ++APF ++ YP+ + Y N   P  + F  F      +  G+   YTN+FDA
Sbjct: 191 KALLQFQSANDAPFMISPYPYFA-YKNQPTPDTLAFCLFQPNAGQVDSGNGHKYTNMFDA 250

Query: 253 NFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKH---ANIQNAKRFNQGLLRHALSGEGTP 312
             D + SAL   G+ D++I+V E GWP  GD +    ++ NAK +   L+ H  S  GTP
Sbjct: 251 QVDAVHSALNAMGFKDIEIVVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHLKSKVGTP 310

Query: 313 ARIG-IIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELD-LSGTEEEKGLVAVE 372
              G  ID YLFSL DE+ K+    S E+++G+F+ DG   Y++  L  T+         
Sbjct: 311 LMPGKSIDTYLFSLYDEDKKT--GASSEKYFGLFKPDGSTTYDVGLLKNTQNPTTPATPT 370

Query: 373 GVRYMLKRWCVLNPNVNDWEGLADSVDYACSLS-DCTALEYGSSC-NQLTAQGNASYAFN 432
                   WCV  P V+D + L  +++YAC    DC  ++ G +C    T + +A+Y  N
Sbjct: 371 PTPKAAGSWCVPKPGVSD-DQLTGNINYACGQGIDCGPIQPGGACFEPNTVKAHAAYVMN 430

Query: 433 MYYQVNSQKSWTCDFSGLAVVTDEDPSNGNCQFP 455
           +YYQ   + SW CDFS  A +T+ +PS G C FP
Sbjct: 431 LYYQSAGRNSWNCDFSQTATLTNTNPSYGACNFP 456

[Close]

BLAST of Sgr030505 vs. ExPASy TrEMBL
Match: A0A6J1DUS6 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111024222 PE=3 SV=1)

HSP 1 Score: 918.3 bits (2372), Expect = 1.4e-263
Identity = 447/493 (90.67%), Postives = 471/493 (95.54%), Query Frame = 0


Query: 1   MSKSGLQDQNECIFTALLLLFLTIVSNGASVGVNWGTMATHQLPPEKVVNMLQDNGFHKL 60
           MSKSGLQ   +CIFTAL+LL+ TI  NG SVGVNWGTMATHQLPPEKVV MLQ+NGF KL
Sbjct: 1   MSKSGLQIHRDCIFTALILLYSTIFPNGESVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60

Query: 61  KLFEAEERILEALIGTDIEVMLAIPNSMLQMMSEDPEAAASWVDANVTTYSYHGGINIK- 120
           KLFEAE+RILEALIG+DIEVMLAIPNSMLQ+MSEDPEAAASWVD+NVTTYSYHGG+NIK 
Sbjct: 61  KLFEAEDRILEALIGSDIEVMLAIPNSMLQIMSEDPEAAASWVDSNVTTYSYHGGVNIKY 120

Query: 121 --LSNEPFLKSYNGTYLQFTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180
             + NEPFLKSYNGTYLQFTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP
Sbjct: 121 VAVGNEPFLKSYNGTYLQFTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180

Query: 181 SAGDFRSDVRDLTIEILHFLSISNAPFTVNIYPFLSLYSNDYFPMDFAFFDGTYKPIRDG 240
           SAG+FRSDVRDLTIEILHFLS +NAPFTVNIYPFLSLYSNDYFP+DFAFFDGTYKPIRDG
Sbjct: 181 SAGEFRSDVRDLTIEILHFLSTNNAPFTVNIYPFLSLYSNDYFPLDFAFFDGTYKPIRDG 240

Query: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHA 300
           DL YTNVFDANFDTLVSALTKAGYP+MKIIVGEVGWPTDGDKHANIQ AKRFNQGLLRHA
Sbjct: 241 DLAYTNVFDANFDTLVSALTKAGYPEMKIIVGEVGWPTDGDKHANIQYAKRFNQGLLRHA 300

Query: 301 LSGEGTPARIGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360
           LSGEGTPAR+GIIDIYLFSLIDENAKSIEPGSFERHWG+FEFDGKPKYELDLSGTE+EKG
Sbjct: 301 LSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWGLFEFDGKPKYELDLSGTEQEKG 360

Query: 361 LVAVEGVRYMLKRWCVLNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASY 420
           LVAVEGVRYM+KRWCVLNPN+NDWEGLAD+VDYACSLSDCTALEYGSSCNQLTAQGNASY
Sbjct: 361 LVAVEGVRYMVKRWCVLNPNLNDWEGLADNVDYACSLSDCTALEYGSSCNQLTAQGNASY 420

Query: 421 AFNMYYQVNSQKSWTCDFSGLAVVTDEDPSNGNCQFPVMIDYTSSSSSPFLLHERFLDLL 480
           AFNMYYQVNSQKSW CDFSGLAVVT+EDPS GNCQFPVMIDYTSSSSSPFL+ ERFLDLL
Sbjct: 421 AFNMYYQVNSQKSWNCDFSGLAVVTEEDPSYGNCQFPVMIDYTSSSSSPFLVDERFLDLL 480

Query: 481 VAVFMGLVVFLFL 491
           VAV MGL+V  F+
Sbjct: 481 VAVIMGLLVSFFV 493

[Close]

BLAST of Sgr030505 vs. ExPASy TrEMBL
Match: A0A6J1HL09 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111465515 PE=3 SV=1)

HSP 1 Score: 872.1 bits (2252), Expect = 1.1e-249
Identity = 422/494 (85.43%), Postives = 457/494 (92.51%), Query Frame = 0


Query: 1   MSKSGLQDQNECIFTALLLLFLTIVSNGASVGVNWGTMATHQLPPEKVVNMLQDNGFHKL 60
           MSKSGLQ      F  +LLLFLTI+ NG +VGVNWGTMATHQLPPEKVV MLQ+NGF KL
Sbjct: 1   MSKSGLQIHRNFTFRTILLLFLTIIPNGETVGVNWGTMATHQLPPEKVVKMLQENGFLKL 60

Query: 61  KLFEAEERILEALIGTDIEVMLAIPNSMLQMMSEDPEAAASWVDANVTTYSYHGGINIK- 120
           KLFEAE+RILEALIGTDIEVMLA+PNSMLQ+MS+D +AAASWVDANVT Y+YHGG+NIK 
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSDDSKAAASWVDANVTAYTYHGGVNIKY 120

Query: 121 --LSNEPFLKSYNGTYLQFTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180
             + NEPFLKSYNGTYLQ TLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP
Sbjct: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180

Query: 181 SAGDFRSDVRDLTIEILHFLSISNAPFTVNIYPFLSLYSNDYFPMDFAFFDGTYKPIRDG 240
           SAGDFR +VRDLTIEILHFLSI+NAPFTVNIYPFLSLY NDYFP+DFAFF GTYKPI+DG
Sbjct: 181 SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFGGTYKPIKDG 240

Query: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHA 300
           DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGE+GWPTDGDKHANIQ+AKRFNQGLLRHA
Sbjct: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA 300

Query: 301 LSGEGTPARIGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360
           LSGEGTPAR+G+ID+YLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTE+EKG
Sbjct: 301 LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEQEKG 360

Query: 361 LVAVEGVRYMLKRWCVLNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASY 420
           L+AVEGV+YM K+WCVLNPNV DWEGLAD++DYACSLSDCTALEYGSSCNQLTA+GNASY
Sbjct: 361 LIAVEGVKYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY 420

Query: 421 AFNMYYQVNSQKSWTCDFSGLAVVTDEDPSNGNCQFPVMIDYTSSSSSPFLLHERFLDLL 480
           AFNMYYQ+NSQK W C+FSGLA+VT++DPS GNCQFP+MIDY SSSSS       FLDLL
Sbjct: 421 AFNMYYQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSS-------FLDLL 480

Query: 481 VAVFMGLVVFLFLE 492
           VA+FMG VVFL LE
Sbjct: 481 VAIFMGFVVFLCLE 487

[Close]

BLAST of Sgr030505 vs. ExPASy TrEMBL
Match: A0A6J1G3H9 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111450439 PE=3 SV=1)

HSP 1 Score: 869.0 bits (2244), Expect = 9.4e-249
Identity = 427/494 (86.44%), Postives = 452/494 (91.50%), Query Frame = 0


Query: 1   MSKSGLQDQNECIFTALLLLFLTIVSNGASVGVNWGTMATHQLPPEKVVNMLQDNGFHKL 60
           MS+S L+     IF AL+LL LT+V NG SVGVNWGTMATHQLPPEKVV MLQ+NGF KL
Sbjct: 1   MSESVLRFHRNWIFIALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60

Query: 61  KLFEAEERILEALIGTDIEVMLAIPNSMLQMMSEDPEAAASWVDANVTTYSYHGGINIK- 120
           KLFEAE+RILEALIGTDIEVMLAIPNSMLQ+MSEDPEAAA WVD+NVT Y+YHGG+ IK 
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY 120

Query: 121 --LSNEPFLKSYNGTYLQFTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180
             + NEPFLKSYNG YLQ TLPALRNIQQALNDAGLGSKVKATIPFNAD YNSPDSNPVP
Sbjct: 121 VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSKVKATIPFNADTYNSPDSNPVP 180

Query: 181 SAGDFRSDVRDLTIEILHFLSISNAPFTVNIYPFLSLYSNDYFPMDFAFFDGTYKPIRDG 240
           SAGDFR DVRDLTIEILHFLSI+NAPFTVNIYPFLSLY NDYFP+DFAFFDGTYK I+DG
Sbjct: 181 SAGDFRPDVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKSIKDG 240

Query: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHA 300
           DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHA
Sbjct: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHA 300

Query: 301 LSGEGTPARIGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360
           LSGEGTPAR+GIIDIYLFSLIDENAKSIEPGSFERHWGIF+FDGKPKYELDLSG E +KG
Sbjct: 301 LSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFKFDGKPKYELDLSGIEAQKG 360

Query: 361 LVAVEGVRYMLKRWCVLNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASY 420
           L+AVEGVRYM+KRWCVLNPNV DWEGLA SVDYACSLSDCTALEYGSSCNQL+A+GNASY
Sbjct: 361 LIAVEGVRYMVKRWCVLNPNVKDWEGLAYSVDYACSLSDCTALEYGSSCNQLSAEGNASY 420

Query: 421 AFNMYYQVNSQKSWTCDFSGLAVVTDEDPSNGNCQFPVMIDYTSSSSSPFLLHERFLDLL 480
           AFNMYYQ+NSQKSW CDFSG+AVVT EDPS G+CQFPVMIDYT SSSS       FLDLL
Sbjct: 421 AFNMYYQMNSQKSWNCDFSGMAVVTQEDPSYGSCQFPVMIDYTFSSSSSSSSSSSFLDLL 480

Query: 481 VAVFMGLVVFLFLE 492
           VA FMG +VFLFLE
Sbjct: 481 VAGFMGFLVFLFLE 494

[Close]

BLAST of Sgr030505 vs. ExPASy TrEMBL
Match: A0A6J1FB83 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111443850 PE=3 SV=1)

HSP 1 Score: 867.5 bits (2240), Expect = 2.7e-248
Identity = 421/494 (85.22%), Postives = 455/494 (92.11%), Query Frame = 0


Query: 1   MSKSGLQDQNECIFTALLLLFLTIVSNGASVGVNWGTMATHQLPPEKVVNMLQDNGFHKL 60
           MSK  LQ     I   +LLLFLTI+ NG SVGVNWGTMATHQLPPEKVV MLQ+NGF KL
Sbjct: 1   MSKFELQIHRNFILRTILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKL 60

Query: 61  KLFEAEERILEALIGTDIEVMLAIPNSMLQMMSEDPEAAASWVDANVTTYSYHGGINIK- 120
           KLFEAE+RILEALIGTDIEVMLA+PNSMLQ+MS+D +AAA WVDANVT Y+YHGG+NIK 
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSDDSKAAAYWVDANVTAYTYHGGVNIKY 120

Query: 121 --LSNEPFLKSYNGTYLQFTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180
             + NEPFLKSYNGTYLQ TLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP
Sbjct: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180

Query: 181 SAGDFRSDVRDLTIEILHFLSISNAPFTVNIYPFLSLYSNDYFPMDFAFFDGTYKPIRDG 240
           SAGDFR +VRDLTIEILHFLSI+NAPFTVNIYPFLSLY NDYFP+DFAFFDGTYKPI+DG
Sbjct: 181 SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDG 240

Query: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHA 300
           DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGE+GWPTDGDKHANIQ+AKRFNQGLLRHA
Sbjct: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA 300

Query: 301 LSGEGTPARIGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360
           LSGEGTPAR+G+ID+YLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTE+EKG
Sbjct: 301 LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEQEKG 360

Query: 361 LVAVEGVRYMLKRWCVLNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASY 420
           L+AVEGVRYM K+WCVLNPNV DWEGLAD++DYACSLSDCTALEYGSSCNQLTA+GNASY
Sbjct: 361 LIAVEGVRYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY 420

Query: 421 AFNMYYQVNSQKSWTCDFSGLAVVTDEDPSNGNCQFPVMIDYTSSSSSPFLLHERFLDLL 480
           AFNMY+Q+NSQK W C+FSGLA+VT++DPS GNCQFP+MIDY SSSSS       FLDLL
Sbjct: 421 AFNMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSS-------FLDLL 480

Query: 481 VAVFMGLVVFLFLE 492
           VA+FMG VVFL LE
Sbjct: 481 VAIFMGFVVFLSLE 487

[Close]

BLAST of Sgr030505 vs. ExPASy TrEMBL
Match: A0A1S3C742 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103497605 PE=3 SV=1)

HSP 1 Score: 866.7 bits (2238), Expect = 4.7e-248
Identity = 426/489 (87.12%), Postives = 453/489 (92.64%), Query Frame = 0


Query: 12  CIF---TALLLLFLTIVSNGASVGVNWGTMATHQLPPEKVVNMLQDNGFHKLKLFEAEER 71
           CIF     LLLL L IV NG+SVGVNWGTMATHQLPPEKVV MLQ+NGFHKLKLFEA++R
Sbjct: 5   CIFRILLVLLLLLLMIVPNGSSVGVNWGTMATHQLPPEKVVKMLQENGFHKLKLFEADDR 64

Query: 72  ILEALIGTDIEVMLAIPNSMLQMMSEDPEAAASWVDANVTTYSYHGGINIK---LSNEPF 131
           ILEALIGTDIEVMLAIPNSML +MS+DP+AAASWVD+NVT Y+YHGG+ IK   + NEPF
Sbjct: 65  ILEALIGTDIEVMLAIPNSMLFLMSQDPQAAASWVDSNVTAYTYHGGVKIKYVAVGNEPF 124

Query: 132 LKSYNGTYLQFTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRSD 191
           LKSYNGTYLQ TLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAG+FR D
Sbjct: 125 LKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGEFRPD 184

Query: 192 VRDLTIEILHFLSISNAPFTVNIYPFLSLYSNDYFPMDFAFFDGT-YKPIRDGDLTYTNV 251
           VRDL IEILH+LSI+NAPFTVNIYPFLSLY NDYFP+DFAFFDGT YKPI+D DLTYTNV
Sbjct: 185 VRDLAIEILHYLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTAYKPIKDRDLTYTNV 244

Query: 252 FDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTP 311
           FDANFDTLVSAL KAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTP
Sbjct: 245 FDANFDTLVSALAKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTP 304

Query: 312 ARIGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLVAVEGV 371
           AR+GIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDL+GTEEEKGL+ VEGV
Sbjct: 305 ARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLAGTEEEKGLIPVEGV 364

Query: 372 RYMLKRWCVLNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASYAFNMYYQ 431
           RYM KRWCVLNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASYAFNMYYQ
Sbjct: 365 RYMGKRWCVLNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASYAFNMYYQ 424

Query: 432 VNSQKSWTCDFSGLAVVTDEDPSNGNCQFPVMIDYT--SSSSSPFLLHERFLDLLVAVFM 491
           VN+QKSW CDF GLAVVT +DPS GNC FP+MIDYT  SSSSS FL+ ERF DLLVA+FM
Sbjct: 425 VNNQKSWNCDFDGLAVVTQQDPSYGNCLFPIMIDYTSSSSSSSSFLVDERFFDLLVAIFM 484

[Close]

BLAST of Sgr030505 vs. TAIR 10
Match: AT3G24330.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 636.0 bits (1639), Expect = 2.6e-182
Identity = 299/442 (67.65%), Postives = 367/442 (83.03%), Query Frame = 0


Query: 26  SNGASVGVNWGTMATHQLPPEKVVNMLQDNGFHKLKLFEAEERILEALIGTDIEVMLAIP 85
           SN ++VGVNWG MA+HQLPPEKVV ML DN F KLKLFEA++ IL+ALIG+DIEVM+ IP
Sbjct: 34  SNTSNVGVNWGIMASHQLPPEKVVKMLMDNSFTKLKLFEADQNILDALIGSDIEVMIGIP 93

Query: 86  NSMLQMMSEDPEAAASWVDANVTTYSYHGGINIK---LSNEPFLKSYNGTYLQFTLPALR 145
           N  L+ M++D   AASWV+ NVT YSY+GG+NIK   + NEPFL++YNGTY++FTLPAL 
Sbjct: 94  NRFLKEMAQDTSVAASWVEENVTAYSYNGGVNIKYIAVGNEPFLQTYNGTYVEFTLPALI 153

Query: 146 NIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRSDVRDLTIEILHFLSISNA 205
           NIQ+AL +A L   VK T+PFNADIY SP++NPVPSAGDFR ++RD TIEI++FL   ++
Sbjct: 154 NIQRALEEADL-KNVKVTVPFNADIYFSPEANPVPSAGDFRPELRDATIEIINFLYSHDS 213

Query: 206 PFTVNIYPFLSLYSNDYFPMDFAFFDGTYKPIRDGDLTYTNVFDANFDTLVSALTKAGYP 265
           PFTVNIYPFLSLY N YFP+DFAFFDGT K +RDG+L YTNVFDAN DTL+ A+ +  + 
Sbjct: 214 PFTVNIYPFLSLYGNAYFPLDFAFFDGTNKSLRDGNLVYTNVFDANLDTLICAMERYSFL 273

Query: 266 DMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARIGII-DIYLFSLIDEN 325
            MKIIVGEVGWPTDGDK+AN+++AKRFNQG+++HA+SG GTPAR G+I D+YLFSL+DE+
Sbjct: 274 GMKIIVGEVGWPTDGDKNANVKSAKRFNQGMVKHAMSGNGTPARKGVIMDVYLFSLVDED 333

Query: 326 AKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLVAVEGVRYMLKRWCVLNPNVNDW 385
           AKSI PG+FERHWGIFEFDG+PKYELDLSG   +K LV VE V+Y+ K WC+L+PN  + 
Sbjct: 334 AKSIAPGTFERHWGIFEFDGRPKYELDLSGKGNDKPLVPVEDVKYLPKTWCILDPNAYNL 393

Query: 386 EGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASYAFNMYYQVNSQKSWTCDFSGLAVV 445
           + L D++DYACSLSDCTAL YGSSCN LTA GN SYAFNMYYQ++ QK+W CDF GL ++
Sbjct: 394 DDLPDNIDYACSLSDCTALGYGSSCNHLTATGNVSYAFNMYYQMHDQKTWDCDFLGLGLI 453

Query: 446 TDEDPSNGNCQFPVMIDYTSSS 464
           TDEDPS+  C+FPVMID   S+
Sbjct: 454 TDEDPSDELCEFPVMIDTGDST 474

[Close]

BLAST of Sgr030505 vs. TAIR 10
Match: AT2G19440.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 531.9 bits (1369), Expect = 5.2e-151
Identity = 255/437 (58.35%), Postives = 321/437 (73.46%), Query Frame = 0


Query: 31  VGVNWGTMATHQLPPEKVVNMLQDNGFHKLKLFEAEERILEALIGTDIEVMLAIPNSMLQ 90
           +GVNWGTMATH+LPP+KVV ML+DN  +K+KLF+A+E  + AL G+ +EVM+AIPN  L+
Sbjct: 21  LGVNWGTMATHKLPPKKVVQMLKDNNINKVKLFDADETTMSALSGSGLEVMVAIPNDQLK 80

Query: 91  MMSEDPEAAASWVDANVTTYSYHGGINI---KLSNEPFLKSYNGTYLQFTLPALRNIQQA 150
           +M    + A  WV  NVT Y+++GG+NI    + NEPFLKSYNG+++  T PAL+NIQ A
Sbjct: 81  VMG-SYDRAKDWVHKNVTRYNFNGGVNITFVAVGNEPFLKSYNGSFINLTFPALQNIQNA 140

Query: 151 LNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRSDVRDLTIEILHFLSISNAPFTVN 210
           LN+AGLGS VKAT+P NAD+Y+SP SNPVPSAG FR D+     +I+ FL  ++AP T+N
Sbjct: 141 LNEAGLGSSVKATVPLNADVYDSPSSNPVPSAGRFRPDIIGQMTQIVDFLGNNSAPITIN 200

Query: 211 IYPFLSLYSNDYFPMDFAFFDGTYKPIRDGDLTYTNVFDANFDTLVSALTKAGYPDMKII 270
           IYPFLSLY ND FP+++AFFDG  KP+ D  + YTNVFDANFDTLVSAL   G+ DM II
Sbjct: 201 IYPFLSLYGNDDFPLNYAFFDGA-KPVDDNGIAYTNVFDANFDTLVSALKAVGHGDMPII 260

Query: 271 VGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARIGIIDIYLFSLIDENAKSIEP 330
           VGEVGWPT+GDKHAN  +A RF  GLL       GTP R   I++YLF L+DE+AKSI P
Sbjct: 261 VGEVGWPTEGDKHANSGSAYRFYNGLLPRLGENRGTPLRPTYIEVYLFGLLDEDAKSIAP 320

Query: 331 GSFERHWGIFEFDGKPKYELDLSGTEEEKGLVAVEGVRYMLKRWCVLNPNVNDWEGLADS 390
           G FERHWGIF+FDG+PK+ +DLSG  + K L+  E V Y  K+WC+ N    D   LA +
Sbjct: 321 GEFERHWGIFKFDGQPKFPIDLSGQGQNKLLIGAENVTYQPKKWCMFNTEAKDLTKLAAN 380

Query: 391 VDYACSLSDCTALEYGSSCNQLTAQGNASYAFNMYYQVNSQKSWTCDFSGLAVVTDEDPS 450
           +DYAC+ SDCTAL YGSSCN L A GNASYAFNMY+QV +Q    C F GLA +T ++ S
Sbjct: 381 IDYACTFSDCTALGYGSSCNTLDANGNASYAFNMYFQVKNQDEDACIFQGLATITTKNIS 440

Query: 451 NGNCQFPVMIDYTSSSS 465
            G C FP+ I  +++SS
Sbjct: 441 QGQCNFPIQIVASTASS 455

[Close]

BLAST of Sgr030505 vs. TAIR 10
Match: AT1G64760.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 527.7 bits (1358), Expect = 9.9e-150
Identity = 260/464 (56.03%), Postives = 333/464 (71.77%), Query Frame = 0


Query: 22  LTIVSNGASVGVNWGTMATHQLPPEKVVNMLQDNGFHKLKLFEAEERILEALIGTDIEVM 81
           L I+ NG  +GVNWGTMATH+LPP+ VV ML+DN  +K+KLF+A+E  + AL G+ +EVM
Sbjct: 18  LGILVNG--LGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGSGLEVM 77

Query: 82  LAIPNSMLQMMSEDPEAAASWVDANVTTYSYHGGINI---KLSNEPFLKSYNGTYLQFTL 141
           +AIPN  L++M+   + A  WV  NVT Y++ GG+NI    + NEPFLKSYNG+++  T 
Sbjct: 78  VAIPNDQLKVMT-SYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTF 137

Query: 142 PALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRSDVRDLTIEILHFLS 201
           PAL NIQ ALN+AGLG+ VKAT+P NAD+Y+SP SNPVPSAG FR D+     +I+ FL 
Sbjct: 138 PALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLG 197

Query: 202 ISNAPFTVNIYPFLSLYSNDYFPMDFAFFDGTYKPIRDGDLTYTNVFDANFDTLVSALTK 261
            +NAP T+NIYPFLSLY ND FP+++AFFDG  +PI D  + YTNVFDANFDTLVS+L  
Sbjct: 198 KNNAPITINIYPFLSLYGNDDFPLNYAFFDGA-EPINDNGIDYTNVFDANFDTLVSSLKA 257

Query: 262 AGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARIGIIDIYLFSLI 321
            G+ DM IIVGEVGWPT+GDKHAN  +A RF  GLL    + +GTP R   I++YLF L+
Sbjct: 258 VGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEVYLFGLL 317

Query: 322 DENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLVAVEGVRYMLKRWCVLNPNV 381
           DE+AKSI PG FERHWGIF+FDG+PK+ +DLSG  + K L+  + V Y+  +WC  NP  
Sbjct: 318 DEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEA 377

Query: 382 NDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASYAFNMYYQVNSQKSWTCDFSGL 441
            D   LA ++DYAC+ SDCTAL YGSSCN L A GNASYAFNM++QV +Q    C F GL
Sbjct: 378 KDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESACYFQGL 437

Query: 442 AVVTDEDPSNGNCQFPVMIDYTSSSSSPFLLHERFLDLLVAVFM 483
           A +T ++ S G C FP+ I   +SS+S F      L +L+  F+
Sbjct: 438 ATITTQNISQGQCNFPIQI--VASSASSFSCSSYSLVVLIVWFL 475

[Close]

BLAST of Sgr030505 vs. TAIR 10
Match: AT1G64760.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 527.7 bits (1358), Expect = 9.9e-150
Identity = 260/464 (56.03%), Postives = 333/464 (71.77%), Query Frame = 0


Query: 22  LTIVSNGASVGVNWGTMATHQLPPEKVVNMLQDNGFHKLKLFEAEERILEALIGTDIEVM 81
           L I+ NG  +GVNWGTMATH+LPP+ VV ML+DN  +K+KLF+A+E  + AL G+ +EVM
Sbjct: 18  LGILVNG--LGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGSGLEVM 77

Query: 82  LAIPNSMLQMMSEDPEAAASWVDANVTTYSYHGGINI---KLSNEPFLKSYNGTYLQFTL 141
           +AIPN  L++M+   + A  WV  NVT Y++ GG+NI    + NEPFLKSYNG+++  T 
Sbjct: 78  VAIPNDQLKVMT-SYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTF 137

Query: 142 PALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRSDVRDLTIEILHFLS 201
           PAL NIQ ALN+AGLG+ VKAT+P NAD+Y+SP SNPVPSAG FR D+     +I+ FL 
Sbjct: 138 PALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLG 197

Query: 202 ISNAPFTVNIYPFLSLYSNDYFPMDFAFFDGTYKPIRDGDLTYTNVFDANFDTLVSALTK 261
            +NAP T+NIYPFLSLY ND FP+++AFFDG  +PI D  + YTNVFDANFDTLVS+L  
Sbjct: 198 KNNAPITINIYPFLSLYGNDDFPLNYAFFDGA-EPINDNGIDYTNVFDANFDTLVSSLKA 257

Query: 262 AGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARIGIIDIYLFSLI 321
            G+ DM IIVGEVGWPT+GDKHAN  +A RF  GLL    + +GTP R   I++YLF L+
Sbjct: 258 VGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEVYLFGLL 317

Query: 322 DENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLVAVEGVRYMLKRWCVLNPNV 381
           DE+AKSI PG FERHWGIF+FDG+PK+ +DLSG  + K L+  + V Y+  +WC  NP  
Sbjct: 318 DEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEA 377

Query: 382 NDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASYAFNMYYQVNSQKSWTCDFSGL 441
            D   LA ++DYAC+ SDCTAL YGSSCN L A GNASYAFNM++QV +Q    C F GL
Sbjct: 378 KDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESACYFQGL 437

Query: 442 AVVTDEDPSNGNCQFPVMIDYTSSSSSPFLLHERFLDLLVAVFM 483
           A +T ++ S G C FP+ I   +SS+S F      L +L+  F+
Sbjct: 438 ATITTQNISQGQCNFPIQI--VASSASSFSCSSYSLVVLIVWFL 475

[Close]

BLAST of Sgr030505 vs. TAIR 10
Match: AT3G04010.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 497.7 bits (1280), Expect = 1.1e-140
Identity = 238/451 (52.77%), Postives = 322/451 (71.40%), Query Frame = 0


Query: 17  LLLLFLTIVSNGAS-VGVNWGTMATHQLPPEKVVNMLQDNGFHKLKLFEAEERILEALIG 76
           +++  +  +S G S +GVNWGTMA+HQLPP+ VV ML+DN   K+KLF+A+   + AL G
Sbjct: 18  IIISAVCFLSGGVSGLGVNWGTMASHQLPPKTVVEMLKDNNIQKVKLFDADTNTMGALAG 77

Query: 77  TDIEVMLAIPNSMLQMMSEDPEAAASWVDANVTTYSYHGGINIK---LSNEPFLKSYNGT 136
           + +EVM+AIPN +L  M  + + A  WV  NV+ ++++ G+ IK   + NEPFL +YNG+
Sbjct: 78  SGVEVMVAIPNDLLLAMG-NYQRAKDWVQRNVSRFNFNNGVKIKYVAVGNEPFLTAYNGS 137

Query: 137 YLQFTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRSDVRDLTIE 196
           ++  T PAL NIQ ALN+AG+G   KAT+P NAD+YNSP  N VPSAG FRSD+     +
Sbjct: 138 FINLTYPALFNIQTALNEAGVGDFTKATVPLNADVYNSPPDNQVPSAGRFRSDIIQEMTQ 197

Query: 197 ILHFLSISNAPFTVNIYPFLSLYSNDYFPMDFAFFDGTYKPIRDGDLTYTNVFDANFDTL 256
           I++FL+ + APFTVNIYPFLSLY +  FP ++AFFDG    + D  + YTNVFDA+FDTL
Sbjct: 198 IVNFLAQNKAPFTVNIYPFLSLYLSSDFPFEYAFFDG-QNTVNDNGVIYTNVFDASFDTL 257

Query: 257 VSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARIGIIDI 316
           +++L    + +M++IVGEVGWPTDGDK+AN+ NA+RF  GLL    +  GTP R G I++
Sbjct: 258 LASLNALNHGNMEVIVGEVGWPTDGDKNANVPNAERFYSGLLPRLANNVGTPMRKGYIEV 317

Query: 317 YLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLVAVEGVRYMLKRWC 376
           YLF  IDE+AKS+ PG+FERHWGIF+FDG+PK+ +D  G  ++K L   + V+Y L +WC
Sbjct: 318 YLFGFIDEDAKSVAPGNFERHWGIFKFDGQPKFPVDFRGQGQKKFLTGAQNVQYFLNQWC 377

Query: 377 VLNPN-VNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASYAFNMYYQVNSQKSW 436
           + NPN   +   L D+++YACS SDCTAL YGSSC  L A GNASYAFNMY+QV +Q++ 
Sbjct: 378 MFNPNGRGNMSRLGDNINYACSHSDCTALGYGSSCGNLDANGNASYAFNMYFQVQNQEAQ 437

Query: 437 TCDFSGLAVVTDEDPSNGNCQFPVMIDYTSS 463
            CDF GLA +T ++ S G C FP+ I   SS
Sbjct: 438 ACDFEGLATITTQNISQGQCNFPIQIGEPSS 466

The following BLAST results are available for this feature:

BLAST of Sgr030505 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022157557.1	2.8e-263	90.67	glucan endo-1,3-beta-glucosidase 8 [Momordica charantia]	[more]
XP_038890697.1	1.3e-255	87.73	glucan endo-1,3-beta-glucosidase 8-like [Benincasa hispida]	[more]
XP_022965692.1	2.3e-249	85.43	glucan endo-1,3-beta-glucosidase 8-like [Cucurbita maxima]	[more]
KAG7021271.1	1.1e-248	85.63	Glucan endo-1,3-beta-glucosidase 8 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022946334.1	1.9e-248	86.44	glucan endo-1,3-beta-glucosidase 8-like isoform X1 [Cucurbita moschata]	[more]

BLAST of Sgr030505 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NKW9	1.4e-148	56.03	Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 ...	[more]
Q93Z08	7.2e-137	51.68	Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 ...	[more]
Q9M088	4.8e-133	50.11	Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 ...	[more]
Q9FGH4	8.9e-95	39.58	Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 ...	[more]
Q94G86	1.2e-59	33.70	Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1	[more]

BLAST of Sgr030505 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DUS6	1.4e-263	90.67	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111024222 ...	[more]
A0A6J1HL09	1.1e-249	85.43	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111465515 PE=...	[more]
A0A6J1G3H9	9.4e-249	86.44	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111450439 P...	[more]
A0A6J1FB83	2.7e-248	85.22	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111443850 P...	[more]
A0A1S3C742	4.7e-248	87.12	(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103497605 PE=3 SV...	[more]

BLAST of Sgr030505 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G24330.1	2.6e-182	67.65	O-Glycosyl hydrolases family 17 protein	[more]
AT2G19440.1	5.2e-151	58.35	O-Glycosyl hydrolases family 17 protein	[more]
AT1G64760.1	9.9e-150	56.03	O-Glycosyl hydrolases family 17 protein	[more]
AT1G64760.2	9.9e-150	56.03	O-Glycosyl hydrolases family 17 protein	[more]
AT3G04010.1	1.1e-140	52.77	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 370..453 e-value: 3.6E-36 score: 136.2
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 371..439 e-value: 1.7E-18 score: 67.1
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 31..348 e-value: 6.8E-65 score: 219.7
IPR000490	Glycoside hydrolase family 17	PROSITE	PS00587	GLYCOSYL_HYDROL_F17	coord: 264..277
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 29..356 e-value: 6.1E-105 score: 352.7
None	No IPR available	GENE3D	1.20.58.1040		coord: 366..463 e-value: 6.5E-9 score: 38.0
None	No IPR available	PANTHER	PTHR32227:SF319	BETA-1,3-GLUCANASE-LIKE PROTEIN	coord: 13..465
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 13..465
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 31..348

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr030505.1	Sgr030505.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds