Sgr030490 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr030490 vs. NCBI nr
Match: AEM42983.1 (cytochrome P450 [Siraitia grosvenorii])

HSP 1 Score: 846.7 bits (2186), Expect = 9.0e-242
Identity = 427/474 (90.08%), Postives = 429/474 (90.51%), Query Frame = 0

Query: 1   MWTPGLAIVAVAVICVTHLIY--------------------------------------- 60
           MWTPGLAIVAVAVICVTHLIY                                       
Sbjct: 6   MWTPGLAIVAVAVICVTHLIYRWRNPKCNGVLPPGSMGFPLIGETIQLIAPGYTLDLPPF 65

Query: 61  ------RYGPIFRTSLLGRPIVVTADPEINNFIYQQEGRSVELWYLDSISKVFKQDGEAR 120
                 RYGPIFRTSLLGRPIVVTADPEINNFIYQQEGRSVELWYLDSISKVFKQDGEAR
Sbjct: 66  IKKRVQRYGPIFRTSLLGRPIVVTADPEINNFIYQQEGRSVELWYLDSISKVFKQDGEAR 125

Query: 121 TTAGGVIHKYLRSITLNHFGSESIKEKLLPDIQRYVNKVLHQWSKQPSVEVQRGTLAMLY 180
           TTAGGVIHKYLRSITLNHFGSESIK+KLLPDIQRYVNKVLHQWSKQPSVEVQRGTLAMLY
Sbjct: 126 TTAGGVIHKYLRSITLNHFGSESIKQKLLPDIQRYVNKVLHQWSKQPSVEVQRGTLAMLY 185

Query: 181 DFNAEKMFGYDAEKSSENISDSLITLADGFMAFPLNIPGTKYHKCLKAQKKLVNMLKGLV 240
           DFNAEKMFGYDAEKSSENISDSLITLADGFMAFPLNIPGTKYHKCLKAQKKLVNMLKGLV
Sbjct: 186 DFNAEKMFGYDAEKSSENISDSLITLADGFMAFPLNIPGTKYHKCLKAQKKLVNMLKGLV 245

Query: 241 KERRQANSTAARGDFLDQALRDIDNEQFLTEEFVVNLLFGILFASGSVSGSLTLMFKLLA 300
           KERRQANSTAARGDFLDQALRDIDNEQFLTEEFVVNLLFGILFASGSVSGSLTLMFKLLA
Sbjct: 246 KERRQANSTAARGDFLDQALRDIDNEQFLTEEFVVNLLFGILFASGSVSGSLTLMFKLLA 305

Query: 301 DNPSVVRELTAEHEAFLKQRKDPQSPITWEEYKSMTFTIQVVNETLRLSNALPFLLRRTV 360
           DNPSVVRELTAEHEAFLKQR+DPQSPITWEEYKSMTFTIQVVNETLRLSNALPFLLRRTV
Sbjct: 306 DNPSVVRELTAEHEAFLKQREDPQSPITWEEYKSMTFTIQVVNETLRLSNALPFLLRRTV 365

Query: 361 KDVNIKGYTIPAGWTLMVANSALHLNPDTHKDPLAFNPWRWKEQDQYSISKNLQPFGGGA 420
           KDVNIKGYTIPAGWTLMVANSALHLNPDTHKDPLAFNPWRWKEQDQYSISKNLQPFGGGA
Sbjct: 366 KDVNIKGYTIPAGWTLMVANSALHLNPDTHKDPLAFNPWRWKEQDQYSISKNLQPFGGGA 425

Query: 421 RQCAGADYSRVFMAIFLHTLVTKYRWTKVKGGEICRSPILKFGDGIHISFSEKA 430
           RQCAGADYSRVFMAIFLHTLVTKYRWTKVKGGEICRSPILKFGDGIHISFSEKA
Sbjct: 426 RQCAGADYSRVFMAIFLHTLVTKYRWTKVKGGEICRSPILKFGDGIHISFSEKA 479

BLAST of Sgr030490 vs. NCBI nr
Match: XP_008459936.1 (PREDICTED: cytochrome P450 87A3-like [Cucumis melo])

HSP 1 Score: 731.9 bits (1888), Expect = 3.2e-207
Identity = 352/410 (85.85%), Postives = 380/410 (92.68%), Query Frame = 0

Query: 20  IYRYGPIFRTSLLGRPIVVTADPEINNFIYQQEGRSVELWYLDSISKVFKQDGEARTTAG 79
           + +YGPIFRTSL+GR IVVTADPEIN+FIY QEGR+VELWYLDSISKVFKQDGEA+TTAG
Sbjct: 74  VQKYGPIFRTSLVGRSIVVTADPEINSFIYNQEGRTVELWYLDSISKVFKQDGEAKTTAG 133

Query: 80  GVIHKYLRSITLNHFGSESIKEKLLPDIQRYVNKVLHQWSKQPSVEVQRGTLAMLYDFNA 139
           G IHKYLRSITLNHFGSES+K KLL DIQRYV+KVL QWS QPSVEVQRGTL MLYDFNA
Sbjct: 134 GAIHKYLRSITLNHFGSESLKSKLLADIQRYVDKVLIQWSNQPSVEVQRGTLTMLYDFNA 193

Query: 140 EKMFGYDAEKSSENISDSLITLADGFMAFPLNIPGTKYHKCLKAQKKLVNMLKGLVKERR 199
           + MFGYD EKS+ENIS+SLITLADGFM+FP+NIPGTKY+KCLKAQKKLVNM K LVKER 
Sbjct: 194 DVMFGYDPEKSNENISESLITLADGFMSFPVNIPGTKYNKCLKAQKKLVNMFKTLVKERH 253

Query: 200 QANSTAARGDFLDQALRDIDNEQFLTEEFVVNLLFGILFASGSVSGSLTLMFKLLADNPS 259
           QA+  AARGDFLDQALRDI+NEQFLTEEFVVNLLFGILFASGSVSGSLTLMFKLLA+NPS
Sbjct: 254 QASVAAARGDFLDQALRDIENEQFLTEEFVVNLLFGILFASGSVSGSLTLMFKLLAENPS 313

Query: 260 VVRELTAEHEAFLKQRKDPQSPITWEEYKSMTFTIQVVNETLRLSNALPFLLRRTVKDVN 319
           VV ELTAEHE FLKQRKDP+SPITWEEYKSMTFT+ V+ E  RLSNA+PFLLRRT KDVN
Sbjct: 314 VVEELTAEHEKFLKQRKDPESPITWEEYKSMTFTLYVIYEIFRLSNAMPFLLRRTTKDVN 373

Query: 320 IKGYTIPAGWTLMVANSALHLNPDTHKDPLAFNPWRWKEQDQYSISKNLQPFGGGARQCA 379
           I+GYTIPAGWT+MVANSALHLNP+THKDPL FNPWRWK+QDQYS+SKNLQPFGGG RQCA
Sbjct: 374 IRGYTIPAGWTIMVANSALHLNPETHKDPLDFNPWRWKDQDQYSVSKNLQPFGGGTRQCA 433

Query: 380 GADYSRVFMAIFLHTLVTKYRWTKVKGGEICRSPILKFGDGIHISFSEKA 430
           GADYSRVFMAIFLHTLVTKY W  VKGGE+ RSPILKFGDGIH+SFS KA
Sbjct: 434 GADYSRVFMAIFLHTLVTKYSWKVVKGGEVSRSPILKFGDGIHVSFSAKA 483

BLAST of Sgr030490 vs. NCBI nr
Match: KAA0039873.1 (cytochrome P450 87A3-like [Cucumis melo var. makuwa] >TYK24628.1 cytochrome P450 87A3-like [Cucumis melo var. makuwa])

HSP 1 Score: 731.9 bits (1888), Expect = 3.2e-207
Identity = 353/408 (86.52%), Postives = 379/408 (92.89%), Query Frame = 0

Query: 22  RYGPIFRTSLLGRPIVVTADPEINNFIYQQEGRSVELWYLDSISKVFKQDGEARTTAGGV 81
           RYGPIFRTSL+GR IVVTADPEIN+FIY QEGR+VELWYLDSISKVFKQDGEA+TTAGG 
Sbjct: 3   RYGPIFRTSLVGRSIVVTADPEINSFIYNQEGRTVELWYLDSISKVFKQDGEAKTTAGGA 62

Query: 82  IHKYLRSITLNHFGSESIKEKLLPDIQRYVNKVLHQWSKQPSVEVQRGTLAMLYDFNAEK 141
           IHKYLRSITLNHFGSES+K KLL DIQRYV+KVL QWS QPSVEVQRGTL MLYDFNA+ 
Sbjct: 63  IHKYLRSITLNHFGSESLKSKLLADIQRYVDKVLIQWSNQPSVEVQRGTLTMLYDFNADV 122

Query: 142 MFGYDAEKSSENISDSLITLADGFMAFPLNIPGTKYHKCLKAQKKLVNMLKGLVKERRQA 201
           MFGYD EKS+ENIS+SLITLADGFM+FP+NIPGTKY+KCLKAQKKLVNM K LVKER QA
Sbjct: 123 MFGYDPEKSNENISESLITLADGFMSFPVNIPGTKYNKCLKAQKKLVNMFKTLVKERHQA 182

Query: 202 NSTAARGDFLDQALRDIDNEQFLTEEFVVNLLFGILFASGSVSGSLTLMFKLLADNPSVV 261
           +  AARGDFLDQALRDI+NEQFLTEEFVVNLLFGILFASGSVSGSLTLMFKLLA+NPSVV
Sbjct: 183 SVAAARGDFLDQALRDIENEQFLTEEFVVNLLFGILFASGSVSGSLTLMFKLLAENPSVV 242

Query: 262 RELTAEHEAFLKQRKDPQSPITWEEYKSMTFTIQVVNETLRLSNALPFLLRRTVKDVNIK 321
            ELTAEHE FLKQRK+P+SPITWEEYKSMTFT+ V+ E  RLSNALPFLLRRT KDVNI+
Sbjct: 243 EELTAEHETFLKQRKNPESPITWEEYKSMTFTLYVIYEIFRLSNALPFLLRRTTKDVNIR 302

Query: 322 GYTIPAGWTLMVANSALHLNPDTHKDPLAFNPWRWKEQDQYSISKNLQPFGGGARQCAGA 381
           GYTIPAGWT+MVANSALHLNP+THKDPL FNPWRWK+QDQYS+SKNLQPFGGG RQCAGA
Sbjct: 303 GYTIPAGWTIMVANSALHLNPETHKDPLDFNPWRWKDQDQYSVSKNLQPFGGGTRQCAGA 362

Query: 382 DYSRVFMAIFLHTLVTKYRWTKVKGGEICRSPILKFGDGIHISFSEKA 430
           DYSRVFMAIFLHTLVTKY W  VKGGE+ RSPILKFGDGIH+SFS KA
Sbjct: 363 DYSRVFMAIFLHTLVTKYSWKVVKGGEVSRSPILKFGDGIHVSFSAKA 410

BLAST of Sgr030490 vs. NCBI nr
Match: XP_023515014.1 (cytochrome P450 87A3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 726.1 bits (1873), Expect = 1.8e-205
Identity = 364/474 (76.79%), Postives = 396/474 (83.54%), Query Frame = 0

Query: 1   MWTPGLAIVAVAVICVTHLIYR-------------------------------------- 60
           MW+PGLAI AV VI +THL+YR                                      
Sbjct: 7   MWSPGLAIGAVVVILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNTLDLPPF 66

Query: 61  -------YGPIFRTSLLGRPIVVTADPEINNFIYQQEGRSVELWYLDSISKVFKQDGEAR 120
                  YGPIFRTSL+GR IVVTADPEIN+FIY QEG++VELWYLDSISKV KQDG+ R
Sbjct: 67  IKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLKQDGDTR 126

Query: 121 TTAGGVIHKYLRSITLNHFGSESIKEKLLPDIQRYVNKVLHQWSKQPSVEVQRGTLAMLY 180
           TTAGGVIHKYLR ITLNHFGSES+K KLL DIQR V+KVL QWS +PSVE+Q GTLAMLY
Sbjct: 127 TTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHGTLAMLY 186

Query: 181 DFNAEKMFGYDAEKSSENISDSLITLADGFMAFPLNIPGTKYHKCLKAQKKLVNMLKGLV 240
           DFNA+ MFGYD EKSSE+IS+SLITLADGFMA PLN+PGTKY+KCLKAQKKLVNM K LV
Sbjct: 187 DFNADVMFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVNMFKDLV 246

Query: 241 KERRQANSTAARGDFLDQALRDIDNEQFLTEEFVVNLLFGILFASGSVSGSLTLMFKLLA 300
           KERRQA +  ARGDFLDQALRDI +EQFL+EEFVVNLLFGILFASGSVSGSLTLM KLLA
Sbjct: 247 KERRQAAAANARGDFLDQALRDIGSEQFLSEEFVVNLLFGILFASGSVSGSLTLMLKLLA 306

Query: 301 DNPSVVRELTAEHEAFLKQRKDPQSPITWEEYKSMTFTIQVVNETLRLSNALPFLLRRTV 360
           +NPSVVRELTAEHEAFLKQRKDP+SPITWEEYKSMTFT+QV+ ETLRLSNALPFLLRRT+
Sbjct: 307 ENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNALPFLLRRTI 366

Query: 361 KDVNIKGYTIPAGWTLMVANSALHLNPDTHKDPLAFNPWRWKEQDQYSISKNLQPFGGGA 420
           KD+NIKGYTIPAGWTLMVANSALHLNP THKDPL FNPWRWK+ DQ+SISKNLQPFGGG+
Sbjct: 367 KDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKNLQPFGGGS 426

Query: 421 RQCAGADYSRVFMAIFLHTLVTKYRWTKVKGGEICRSPILKFGDGIHISFSEKA 430
           RQCAGADYSRVFMAIFLHTL+TKY W KVKGG+I RSPILKFGDG+HISF  KA
Sbjct: 427 RQCAGADYSRVFMAIFLHTLLTKYSWRKVKGGDISRSPILKFGDGLHISFKAKA 480

BLAST of Sgr030490 vs. NCBI nr
Match: XP_022960197.1 (cytochrome P450 87A3-like [Cucurbita moschata])

HSP 1 Score: 725.7 bits (1872), Expect = 2.3e-205
Identity = 363/474 (76.58%), Postives = 396/474 (83.54%), Query Frame = 0

Query: 1   MWTPGLAIVAVAVICVTHLIYR-------------------------------------- 60
           MW+PGLAI AV +I +THL+YR                                      
Sbjct: 7   MWSPGLAIGAVVIILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNTLDLPPF 66

Query: 61  -------YGPIFRTSLLGRPIVVTADPEINNFIYQQEGRSVELWYLDSISKVFKQDGEAR 120
                  YGPIFRTSL+GR IVVTADPEIN+FIY QEG++VELWYLDSISKV KQDG+ R
Sbjct: 67  IKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLKQDGDTR 126

Query: 121 TTAGGVIHKYLRSITLNHFGSESIKEKLLPDIQRYVNKVLHQWSKQPSVEVQRGTLAMLY 180
           TTAGGVIHKYLR ITLNHFGSES+K KLL DIQR V+KVL QWS +PSVE+Q GTLAMLY
Sbjct: 127 TTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHGTLAMLY 186

Query: 181 DFNAEKMFGYDAEKSSENISDSLITLADGFMAFPLNIPGTKYHKCLKAQKKLVNMLKGLV 240
           DFNA+ MFGYD EKSSE+IS+SLITLADGFMA PLN+PGTKY+KCLKAQKKLVNM K LV
Sbjct: 187 DFNADVMFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVNMFKDLV 246

Query: 241 KERRQANSTAARGDFLDQALRDIDNEQFLTEEFVVNLLFGILFASGSVSGSLTLMFKLLA 300
           KERRQA +  ARGDFLDQALRDI +EQFL+EEFVVNLLFGILFASGSVSGSLTLM KLLA
Sbjct: 247 KERRQAAAANARGDFLDQALRDIGSEQFLSEEFVVNLLFGILFASGSVSGSLTLMLKLLA 306

Query: 301 DNPSVVRELTAEHEAFLKQRKDPQSPITWEEYKSMTFTIQVVNETLRLSNALPFLLRRTV 360
           +NPSVVRELTAEHEAFLKQRKDP+SPITWEEYKSMTFT+QV+ ETLRLSNALPFLLRRT+
Sbjct: 307 ENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNALPFLLRRTI 366

Query: 361 KDVNIKGYTIPAGWTLMVANSALHLNPDTHKDPLAFNPWRWKEQDQYSISKNLQPFGGGA 420
           KD+NIKGYTIPAGWTLMVANSALHLNP THKDPL FNPWRWK+ DQ+SISKNLQPFGGG+
Sbjct: 367 KDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKNLQPFGGGS 426

Query: 421 RQCAGADYSRVFMAIFLHTLVTKYRWTKVKGGEICRSPILKFGDGIHISFSEKA 430
           RQCAGADYSRVFMAIFLHTL+TKY W KVKGG+I RSPILKFGDG+HISF  KA
Sbjct: 427 RQCAGADYSRVFMAIFLHTLLTKYSWRKVKGGDISRSPILKFGDGLHISFKAKA 480

BLAST of Sgr030490 vs. ExPASy Swiss-Prot
Match: K7NBR2 (Cucurbitadienol 11-hydroxylase OS=Siraitia grosvenorii OX=190515 GN=CYP87D18 PE=1 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 2.1e-132
Identity = 227/410 (55.37%), Postives = 295/410 (71.95%), Query Frame = 0

Query: 20  IYRYGPIFRTSLLGRPIVVTADPEINNFIYQQEGRSVELWYLDSISKVFKQDGEARTTAG 79
           + RYGPIF+T L GRP+VV+AD E NN+I  QEGR+VE+WYLD++SK F  D E    A 
Sbjct: 65  VERYGPIFKTCLAGRPVVVSADAEFNNYIMLQEGRAVEMWYLDTLSKFFGLDTE-WLKAL 124

Query: 80  GVIHKYLRSITLNHFGSESIKEKLLPDIQRYVNKVLHQWSKQPSVEVQRGTLAMLYDFNA 139
           G+IHKY+RSITLNHFG+E+++E+ LP I+    + LH WS QPSVEV+  +  M++  + 
Sbjct: 125 GLIHKYIRSITLNHFGAEALRERFLPFIEASSMEALHSWSTQPSVEVKNASALMVFRTSV 184

Query: 140 EKMFGYDAEKSSENISDSLITLADGFMAFPLNIPGTKYHKCLKAQKKLVNMLKGLVKERR 199
            KMFG DA+K S NI      L  GF++ PLN PGT YHKCLK  K++   L+ +V + R
Sbjct: 185 NKMFGEDAKKLSGNIPGKFTKLLGGFLSLPLNFPGTTYHKCLKDMKEIQKKLREVVDD-R 244

Query: 200 QANSTAARGDFLDQALRDIDNEQFLTEEFVVNLLFGILFAS-GSVSGSLTLMFKLLADNP 259
            AN      DFL QAL+D ++E+F++EEF++ LLF I FAS  S+S +LTL+ KLL ++P
Sbjct: 245 LANVGPDVEDFLGQALKDKESEKFISEEFIIQLLFSISFASFESISTTLTLILKLLDEHP 304

Query: 260 SVVRELTAEHEAFLKQRKDPQSPITWEEYKSMTFTIQVVNETLRLSNALPFLLRRTVKDV 319
            VV+EL AEHEA  K R DP  PITWEEYKSMTFT+QV+NETLRL +  P LLR+TVKD+
Sbjct: 305 EVVKELEAEHEAIRKARADPDGPITWEEYKSMTFTLQVINETLRLGSVTPALLRKTVKDL 364

Query: 320 NIKGYTIPAGWTLMVANSALHLNPDTHKDPLAFNPWRWKEQDQYSISKNLQPFGGGARQC 379
            +KGY IP GWT+M+  ++ H +P  +KDP  FNPWRWK+ D  +I KN  PFGGG R C
Sbjct: 365 QVKGYIIPEGWTIMLVTASRHRDPKVYKDPHIFNPWRWKDLDSITIQKNFMPFGGGLRHC 424

Query: 380 AGADYSRVFMAIFLHTLVTKYRWTKVKGGEICRSPILKFGDGIHISFSEK 429
           AGA+YS+V++  FLH L TKYRWTK+ GG I R+ IL F DG+H+ F+ K
Sbjct: 425 AGAEYSKVYLCTFLHILCTKYRWTKLGGGTIARAHILSFEDGLHVKFTPK 472

BLAST of Sgr030490 vs. ExPASy Swiss-Prot
Match: Q7XU38 (Cytochrome P450 87A3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP87A3 PE=2 SV=3)

HSP 1 Score: 390.6 bits (1002), Expect = 2.3e-107
Identity = 190/417 (45.56%), Postives = 275/417 (65.95%), Query Frame = 0

Query: 16  VTHLIYRYGPIFRTSLLGRPIVVTADPEINNFIYQQEGRSVELWYLDSISKVFKQDGEAR 75
           V   I RYG IF+TS++GRP+VV+ADPE+N +++QQEG+  E WY D+ +++F +D    
Sbjct: 100 VKERIKRYGSIFKTSVVGRPVVVSADPEMNYYVFQQEGKLFESWYPDTFTEIFGRDNVG- 159

Query: 76  TTAGGVIHKYLRSITLNHFGSESIKEKLLPDIQRYVNKVLHQWSKQPSVEVQRGTLAMLY 135
            +  G ++KYL+++ L  +G E++K  LL +        L  W+ QPSVE++ G   M++
Sbjct: 160 -SLHGFMYKYLKTLVLRLYGQENLKSVLLAETDAACRGSLASWASQPSVELKEGISTMIF 219

Query: 136 DFNAEKMFGYDAEKSSE-NISDSLITLADGFMAFPLNIPGTKYHKCLKAQKKLVNMLKGL 195
           D  A+K+ GYD  K S+ N+  +      G ++FPLNIPGT YH+C++ +K  + +L+G+
Sbjct: 220 DLTAKKLIGYDPSKPSQVNLRKNFGAFICGLISFPLNIPGTAYHECMEGRKNAMKVLRGM 279

Query: 196 VKERRQANSTAARGDFLDQALRDIDNEQ-FLTEEFVVNLLFGILFASGSVSG-SLTLMFK 255
           +KE R A       DF D  ++++  E+  LTE   ++L+F +LFAS   +  +LT+  K
Sbjct: 280 MKE-RMAEPERPCEDFFDHVIQELRREKPLLTETIALDLMFVLLFASFETTALALTIGVK 339

Query: 256 LLADNPSVVRELTAEHEAFLKQRKDPQSPITWEEYKSMTFTIQVVNETLRLSNALPFLLR 315
           LL +NP VV  L  EHEA ++ RKDP S +TW EYKSMTFT QV+ E +RL+N +P + R
Sbjct: 340 LLTENPKVVDALREEHEAIIRNRKDPNSGVTWAEYKSMTFTSQVIMEIVRLANIVPGIFR 399

Query: 316 RTVKDVNIKGYTIPAGWTLMVANSALHLNPDTHKDPLAFNPWRWKEQDQYS-ISKNLQPF 375
           + ++DV IKGYTIPAGW +MV   A+HLNP+ ++DPLAFNPWRW+ + + +  +K+   F
Sbjct: 400 KALQDVEIKGYTIPAGWGIMVCPPAVHLNPEIYEDPLAFNPWRWQGKPEITGGTKHFMAF 459

Query: 376 GGGARQCAGADYSRVFMAIFLHTLVTKYRWTKVKGGEICRSPILKFGDGIHISFSEK 429
           GGG R C G D S+V MA F+H+LVTKY W  VKGG I R+P L F DG HI    K
Sbjct: 460 GGGLRFCVGTDLSKVLMATFIHSLVTKYSWRTVKGGNIVRTPGLSFPDGFHIQLFPK 513

BLAST of Sgr030490 vs. ExPASy Swiss-Prot
Match: Q8L7D5 (Cytochrome P450 708A2 OS=Arabidopsis thaliana OX=3702 GN=CYP708A2 PE=2 SV=3)

HSP 1 Score: 260.8 bits (665), Expect = 2.8e-68
Identity = 141/410 (34.39%), Postives = 230/410 (56.10%), Query Frame = 0

Query: 22  RYGPIFRTSLLGRPIVVTADPEINNFIYQQEGRSVELWYLDSISKVFKQDGEARTTAGGV 81
           +YGP+FRT++ G   VV  +P+I   +++QE +S    Y ++  K F +  E      G 
Sbjct: 70  KYGPLFRTNIFGSNTVVLTEPDIIFEVFRQENKSFVFSYPEAFVKPFGK--ENVFLKHGN 129

Query: 82  IHKYLRSITLNHFGSESIKEKLLPDIQRYVNKVLHQWSKQPSVEVQRGTLAMLYDFNAEK 141
           IHK+++ I+L H GSE++K+K++ +I R   + L   + Q S + +    +++      K
Sbjct: 130 IHKHVKQISLQHLGSEALKKKMIGEIDRVTYEHLRSKANQGSFDAKEAVESVIMAHLTPK 189

Query: 142 MFGYDAEKSSENISDSLITLADGFMAFPLNIPG-TKYHKCLKAQKKLVNMLKGLVKERRQ 201
           +      ++   + D+++ L   +   PL +      +K   A++  + ++K +   R+ 
Sbjct: 190 IISNLKPETQATLVDNIMALGSEWFQSPLKLTTLISIYKVFIARRYALQVIKDVFTRRKA 249

Query: 202 ANSTAARGDFLDQALRDIDNEQFL-TEEFVVNLLFGIL-FASGSVSGSLTLMFKLLADNP 261
           +      GDFLD  + + + E  +  EE  +NL+F IL  A  S S   +L  K LA+N 
Sbjct: 250 SREMC--GDFLDTMVEEGEKEDVIFNEESAINLIFAILVVAKESTSSVTSLAIKFLAENH 309

Query: 262 SVVRELTAEHEAFLKQRKDPQSPITWEEYK-SMTFTIQVVNETLRLSNALPFLLRRTVKD 321
             + EL  EH A L+ R    + ++WEEY+  MTFT  V+NETLR++N  P + R+ V D
Sbjct: 310 KALAELKREHAAILQNRNGKGAGVSWEEYRHQMTFTNMVINETLRMANMAPIMYRKAVND 369

Query: 322 VNIKGYTIPAGWTLMVANSALHLNPDTHKDPLAFNPWRWKEQDQYSISKNLQPFGGGARQ 381
           V IKGYTIPAGW + V   A+H N   +++PL FNPWRW+ ++  S SK    FGGG RQ
Sbjct: 370 VEIKGYTIPAGWIVAVIPPAVHFNDAIYENPLEFNPWRWEGKELRSGSKTFMVFGGGVRQ 429

Query: 382 CAGADYSRVFMAIFLHTLVTKYRWTKVKGGEICRSPILKFGDGIHISFSE 428
           C GA+++R+ ++IF+H LVT Y ++  +  E  R+P+  F  G+ I  S+
Sbjct: 430 CVGAEFARLQISIFIHHLVTTYDFSLAQESEFIRAPLPYFPKGLPIKISQ 475

BLAST of Sgr030490 vs. ExPASy Swiss-Prot
Match: Q5CCK3 (Cytochrome P450 90B2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90B2 PE=1 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 3.4e-66
Identity = 142/414 (34.30%), Postives = 227/414 (54.83%), Query Frame = 0

Query: 20  IYRYGPIFRTSLLGRPIVVTADPEINNFIYQQEGRSVELWYLDSISKVFKQDGEARTTAG 79
           I RYG I+R+SL G   VV+AD  +N +I Q EGR  E  Y  SI  +  +   +     
Sbjct: 87  IARYGKIYRSSLFGERTVVSADAGLNRYILQNEGRLFECSYPRSIGGILGK--WSMLVLV 146

Query: 80  GVIHKYLRSITLNHFGSESIKEKLLPDIQRYVNKVLHQWSKQPSVEVQRGTLAMLYDFNA 139
           G  H+ +R+I+LN   S  ++  LLP+++R+   VL  W    +   Q       ++  A
Sbjct: 147 GDPHREMRAISLNFLSSVRLRAVLLPEVERHTLLVLRAWPPSSTFSAQHQAKKFTFNLMA 206

Query: 140 EKMFGYD-AEKSSENISDSLITLADGFMAFPLNIPGTKYHKCLKAQKKLVNMLKGLVKER 199
           + +   D  E+ +E +    IT   G ++ PLN+PGT Y K LK++  ++ +++  ++ER
Sbjct: 207 KNIMSMDPGEEETERLRREYITFMKGVVSAPLNLPGTPYWKALKSRAAILGVIERKMEER 266

Query: 200 ----RQANSTAARGDFLDQALRDIDNEQFLTEEFVVNLLFGILFASGSVSG-SLTLMFKL 259
                + +++  + D L  AL+    +  L++E +++LL  +LFA    S  +L L    
Sbjct: 267 VEKLSKEDASVEQDDLLGWALK----QSNLSKEQILDLLLSLLFAGHETSSMALALAIFF 326

Query: 260 LADNPSVVRELTAEHEAFL-KQRKDPQSPITWEEYKSMTFTIQVVNETLRLSNALPFLLR 319
           L   P  V+EL  EH     +QR   +  ++WE+YK M FT  V+NETLRL N + FL R
Sbjct: 327 LEGCPKAVQELREEHLGIARRQRLRGECKLSWEDYKEMVFTQCVINETLRLGNVVRFLHR 386

Query: 320 RTVKDVNIKGYTIPAGWTLMVANSALHLNPDTHKDPLAFNPWRWKEQDQ---YSISKNLQ 379
           + +KDV+ KGY IP+GW ++   +A+HL+   ++DP  FNPWRWK        + S +  
Sbjct: 387 KVIKDVHYKGYDIPSGWKILPVLAAVHLDSSLYEDPQRFNPWRWKSSGSSGGLAQSSSFM 446

Query: 380 PFGGGARQCAGADYSRVFMAIFLHTLVTKYRWTKVKGGEICRSPILKFGDGIHI 424
           P+GGG R CAG++ +++ MA+FLH LV  +RW   +  +    P + F  G+ I
Sbjct: 447 PYGGGTRLCAGSELAKLEMAVFLHHLVLNFRWELAEPDQAFVFPFVDFPKGLPI 494

BLAST of Sgr030490 vs. ExPASy Swiss-Prot
Match: Q8GSQ1 (Cytochrome P450 85A1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP85A1 PE=1 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 4.1e-64
Identity = 139/407 (34.15%), Postives = 221/407 (54.30%), Query Frame = 0

Query: 22  RYGPIFRTSLLGRPIVVTADPEINNFIYQQEGRSVELWYLDSISKVFKQDGEARTTAGGV 81
           RYG +FRT +LG P VV  + E+N      EGR     Y  S+  +  ++  A     G 
Sbjct: 64  RYGSVFRTHILGCPTVVCMEAELNRRALASEGRGFVPGYPQSMLDILGRNNIA--AVQGP 123

Query: 82  IHKYLRSITLNHFGSESIKEKLLPDIQRYVNKVLHQWSKQPS---VEVQRGTLAMLYDFN 141
           +H+ +R   L+      I+  LLP I  ++   L  WS   S   V++Q  T  M     
Sbjct: 124 LHRAMRGAMLSLVRPAMIRSSLLPKIDAFMRSHLAAWSSSSSSAVVDIQAKTKEMALLSA 183

Query: 142 AEKMFGYDAEKSSENISDSLITLADGFMAFPLNIPGTKYHKCLKAQKKLVNMLKGLVKER 201
             ++ G  A   S+ +   L TL  G ++ P+N+PGT Y++  KA+KKLV ML+ ++ ER
Sbjct: 184 LRQIAGVSAGPLSDALKAELYTLVLGTISLPINLPGTNYYQGFKARKKLVAMLEQMIAER 243

Query: 202 RQANSTAARGDFLDQALRDID-NEQFLTEEFVVNLLFGILFASGSVSGSLTLM-FKLLAD 261
           R  +S     D LD  L  ++   + LT+E +++L+  ++++      + ++M  K L+D
Sbjct: 244 R--SSGQVHDDMLDALLTGVEGTREKLTDEQIIDLIITLIYSGYETMSTTSMMAVKYLSD 303

Query: 262 NPSVVRELTAEHEAFLKQRKDPQSPITWEEYKSMTFTIQVVNETLRLSNALPFLLRRTVK 321
           +P  + +L  EH   +++ K P+  I W ++KSMTFT  V+ ETLRL+  +  LLR+T +
Sbjct: 304 HPKALEQLRKEH-FDIRKGKAPEDAIDWNDFKSMTFTRAVIFETLRLATVVNGLLRKTTQ 363

Query: 322 DVNIKGYTIPAGWTLMVANSALHLNPDTHKDPLAFNPWRWKEQDQYSISKNLQPFGGGAR 381
           DV + GY IP GW + V    ++ +P  + DP+ FNPWRW E++  S   +   FGGG+R
Sbjct: 364 DVEMNGYVIPKGWRIYVYTREINYDPFLYPDPMTFNPWRWLEKNMES-HPHFMLFGGGSR 423

Query: 382 QCAGADYSRVFMAIFLHTLVTKYRWTKVKGGEICRSPILKFGDGIHI 424
            C G +   V +A FLH  VT+YRW +     I + P ++  +G+HI
Sbjct: 424 MCPGKEVGTVEIATFLHYFVTQYRWEEEGNNTILKFPRVEAPNGLHI 464

BLAST of Sgr030490 vs. ExPASy TrEMBL
Match: K7NBK8 (Cytochrome P450 OS=Siraitia grosvenorii OX=190515 GN=P450-2 PE=2 SV=1)

HSP 1 Score: 846.7 bits (2186), Expect = 4.4e-242
Identity = 427/474 (90.08%), Postives = 429/474 (90.51%), Query Frame = 0

Query: 1   MWTPGLAIVAVAVICVTHLIY--------------------------------------- 60
           MWTPGLAIVAVAVICVTHLIY                                       
Sbjct: 6   MWTPGLAIVAVAVICVTHLIYRWRNPKCNGVLPPGSMGFPLIGETIQLIAPGYTLDLPPF 65

Query: 61  ------RYGPIFRTSLLGRPIVVTADPEINNFIYQQEGRSVELWYLDSISKVFKQDGEAR 120
                 RYGPIFRTSLLGRPIVVTADPEINNFIYQQEGRSVELWYLDSISKVFKQDGEAR
Sbjct: 66  IKKRVQRYGPIFRTSLLGRPIVVTADPEINNFIYQQEGRSVELWYLDSISKVFKQDGEAR 125

Query: 121 TTAGGVIHKYLRSITLNHFGSESIKEKLLPDIQRYVNKVLHQWSKQPSVEVQRGTLAMLY 180
           TTAGGVIHKYLRSITLNHFGSESIK+KLLPDIQRYVNKVLHQWSKQPSVEVQRGTLAMLY
Sbjct: 126 TTAGGVIHKYLRSITLNHFGSESIKQKLLPDIQRYVNKVLHQWSKQPSVEVQRGTLAMLY 185

Query: 181 DFNAEKMFGYDAEKSSENISDSLITLADGFMAFPLNIPGTKYHKCLKAQKKLVNMLKGLV 240
           DFNAEKMFGYDAEKSSENISDSLITLADGFMAFPLNIPGTKYHKCLKAQKKLVNMLKGLV
Sbjct: 186 DFNAEKMFGYDAEKSSENISDSLITLADGFMAFPLNIPGTKYHKCLKAQKKLVNMLKGLV 245

Query: 241 KERRQANSTAARGDFLDQALRDIDNEQFLTEEFVVNLLFGILFASGSVSGSLTLMFKLLA 300
           KERRQANSTAARGDFLDQALRDIDNEQFLTEEFVVNLLFGILFASGSVSGSLTLMFKLLA
Sbjct: 246 KERRQANSTAARGDFLDQALRDIDNEQFLTEEFVVNLLFGILFASGSVSGSLTLMFKLLA 305

Query: 301 DNPSVVRELTAEHEAFLKQRKDPQSPITWEEYKSMTFTIQVVNETLRLSNALPFLLRRTV 360
           DNPSVVRELTAEHEAFLKQR+DPQSPITWEEYKSMTFTIQVVNETLRLSNALPFLLRRTV
Sbjct: 306 DNPSVVRELTAEHEAFLKQREDPQSPITWEEYKSMTFTIQVVNETLRLSNALPFLLRRTV 365

Query: 361 KDVNIKGYTIPAGWTLMVANSALHLNPDTHKDPLAFNPWRWKEQDQYSISKNLQPFGGGA 420
           KDVNIKGYTIPAGWTLMVANSALHLNPDTHKDPLAFNPWRWKEQDQYSISKNLQPFGGGA
Sbjct: 366 KDVNIKGYTIPAGWTLMVANSALHLNPDTHKDPLAFNPWRWKEQDQYSISKNLQPFGGGA 425

Query: 421 RQCAGADYSRVFMAIFLHTLVTKYRWTKVKGGEICRSPILKFGDGIHISFSEKA 430
           RQCAGADYSRVFMAIFLHTLVTKYRWTKVKGGEICRSPILKFGDGIHISFSEKA
Sbjct: 426 RQCAGADYSRVFMAIFLHTLVTKYRWTKVKGGEICRSPILKFGDGIHISFSEKA 479

BLAST of Sgr030490 vs. ExPASy TrEMBL
Match: A0A5A7TA02 (Cytochrome P450 87A3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G001750 PE=3 SV=1)

HSP 1 Score: 731.9 bits (1888), Expect = 1.6e-207
Identity = 353/408 (86.52%), Postives = 379/408 (92.89%), Query Frame = 0

Query: 22  RYGPIFRTSLLGRPIVVTADPEINNFIYQQEGRSVELWYLDSISKVFKQDGEARTTAGGV 81
           RYGPIFRTSL+GR IVVTADPEIN+FIY QEGR+VELWYLDSISKVFKQDGEA+TTAGG 
Sbjct: 3   RYGPIFRTSLVGRSIVVTADPEINSFIYNQEGRTVELWYLDSISKVFKQDGEAKTTAGGA 62

Query: 82  IHKYLRSITLNHFGSESIKEKLLPDIQRYVNKVLHQWSKQPSVEVQRGTLAMLYDFNAEK 141
           IHKYLRSITLNHFGSES+K KLL DIQRYV+KVL QWS QPSVEVQRGTL MLYDFNA+ 
Sbjct: 63  IHKYLRSITLNHFGSESLKSKLLADIQRYVDKVLIQWSNQPSVEVQRGTLTMLYDFNADV 122

Query: 142 MFGYDAEKSSENISDSLITLADGFMAFPLNIPGTKYHKCLKAQKKLVNMLKGLVKERRQA 201
           MFGYD EKS+ENIS+SLITLADGFM+FP+NIPGTKY+KCLKAQKKLVNM K LVKER QA
Sbjct: 123 MFGYDPEKSNENISESLITLADGFMSFPVNIPGTKYNKCLKAQKKLVNMFKTLVKERHQA 182

Query: 202 NSTAARGDFLDQALRDIDNEQFLTEEFVVNLLFGILFASGSVSGSLTLMFKLLADNPSVV 261
           +  AARGDFLDQALRDI+NEQFLTEEFVVNLLFGILFASGSVSGSLTLMFKLLA+NPSVV
Sbjct: 183 SVAAARGDFLDQALRDIENEQFLTEEFVVNLLFGILFASGSVSGSLTLMFKLLAENPSVV 242

Query: 262 RELTAEHEAFLKQRKDPQSPITWEEYKSMTFTIQVVNETLRLSNALPFLLRRTVKDVNIK 321
            ELTAEHE FLKQRK+P+SPITWEEYKSMTFT+ V+ E  RLSNALPFLLRRT KDVNI+
Sbjct: 243 EELTAEHETFLKQRKNPESPITWEEYKSMTFTLYVIYEIFRLSNALPFLLRRTTKDVNIR 302

Query: 322 GYTIPAGWTLMVANSALHLNPDTHKDPLAFNPWRWKEQDQYSISKNLQPFGGGARQCAGA 381
           GYTIPAGWT+MVANSALHLNP+THKDPL FNPWRWK+QDQYS+SKNLQPFGGG RQCAGA
Sbjct: 303 GYTIPAGWTIMVANSALHLNPETHKDPLDFNPWRWKDQDQYSVSKNLQPFGGGTRQCAGA 362

Query: 382 DYSRVFMAIFLHTLVTKYRWTKVKGGEICRSPILKFGDGIHISFSEKA 430
           DYSRVFMAIFLHTLVTKY W  VKGGE+ RSPILKFGDGIH+SFS KA
Sbjct: 363 DYSRVFMAIFLHTLVTKYSWKVVKGGEVSRSPILKFGDGIHVSFSAKA 410

BLAST of Sgr030490 vs. ExPASy TrEMBL
Match: A0A1S3CCK2 (cytochrome P450 87A3-like OS=Cucumis melo OX=3656 GN=LOC103498905 PE=3 SV=1)

HSP 1 Score: 731.9 bits (1888), Expect = 1.6e-207
Identity = 352/410 (85.85%), Postives = 380/410 (92.68%), Query Frame = 0

Query: 20  IYRYGPIFRTSLLGRPIVVTADPEINNFIYQQEGRSVELWYLDSISKVFKQDGEARTTAG 79
           + +YGPIFRTSL+GR IVVTADPEIN+FIY QEGR+VELWYLDSISKVFKQDGEA+TTAG
Sbjct: 74  VQKYGPIFRTSLVGRSIVVTADPEINSFIYNQEGRTVELWYLDSISKVFKQDGEAKTTAG 133

Query: 80  GVIHKYLRSITLNHFGSESIKEKLLPDIQRYVNKVLHQWSKQPSVEVQRGTLAMLYDFNA 139
           G IHKYLRSITLNHFGSES+K KLL DIQRYV+KVL QWS QPSVEVQRGTL MLYDFNA
Sbjct: 134 GAIHKYLRSITLNHFGSESLKSKLLADIQRYVDKVLIQWSNQPSVEVQRGTLTMLYDFNA 193

Query: 140 EKMFGYDAEKSSENISDSLITLADGFMAFPLNIPGTKYHKCLKAQKKLVNMLKGLVKERR 199
           + MFGYD EKS+ENIS+SLITLADGFM+FP+NIPGTKY+KCLKAQKKLVNM K LVKER 
Sbjct: 194 DVMFGYDPEKSNENISESLITLADGFMSFPVNIPGTKYNKCLKAQKKLVNMFKTLVKERH 253

Query: 200 QANSTAARGDFLDQALRDIDNEQFLTEEFVVNLLFGILFASGSVSGSLTLMFKLLADNPS 259
           QA+  AARGDFLDQALRDI+NEQFLTEEFVVNLLFGILFASGSVSGSLTLMFKLLA+NPS
Sbjct: 254 QASVAAARGDFLDQALRDIENEQFLTEEFVVNLLFGILFASGSVSGSLTLMFKLLAENPS 313

Query: 260 VVRELTAEHEAFLKQRKDPQSPITWEEYKSMTFTIQVVNETLRLSNALPFLLRRTVKDVN 319
           VV ELTAEHE FLKQRKDP+SPITWEEYKSMTFT+ V+ E  RLSNA+PFLLRRT KDVN
Sbjct: 314 VVEELTAEHEKFLKQRKDPESPITWEEYKSMTFTLYVIYEIFRLSNAMPFLLRRTTKDVN 373

Query: 320 IKGYTIPAGWTLMVANSALHLNPDTHKDPLAFNPWRWKEQDQYSISKNLQPFGGGARQCA 379
           I+GYTIPAGWT+MVANSALHLNP+THKDPL FNPWRWK+QDQYS+SKNLQPFGGG RQCA
Sbjct: 374 IRGYTIPAGWTIMVANSALHLNPETHKDPLDFNPWRWKDQDQYSVSKNLQPFGGGTRQCA 433

Query: 380 GADYSRVFMAIFLHTLVTKYRWTKVKGGEICRSPILKFGDGIHISFSEKA 430
           GADYSRVFMAIFLHTLVTKY W  VKGGE+ RSPILKFGDGIH+SFS KA
Sbjct: 434 GADYSRVFMAIFLHTLVTKYSWKVVKGGEVSRSPILKFGDGIHVSFSAKA 483

BLAST of Sgr030490 vs. ExPASy TrEMBL
Match: A0A6J1H6S4 (cytochrome P450 87A3-like OS=Cucurbita moschata OX=3662 GN=LOC111461007 PE=3 SV=1)

HSP 1 Score: 725.7 bits (1872), Expect = 1.1e-205
Identity = 363/474 (76.58%), Postives = 396/474 (83.54%), Query Frame = 0

Query: 1   MWTPGLAIVAVAVICVTHLIYR-------------------------------------- 60
           MW+PGLAI AV +I +THL+YR                                      
Sbjct: 7   MWSPGLAIGAVVIILLTHLVYRWRNPKCNGVLPPGSMGFPLIGETIQLIASGNTLDLPPF 66

Query: 61  -------YGPIFRTSLLGRPIVVTADPEINNFIYQQEGRSVELWYLDSISKVFKQDGEAR 120
                  YGPIFRTSL+GR IVVTADPEIN+FIY QEG++VELWYLDSISKV KQDG+ R
Sbjct: 67  IKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLKQDGDTR 126

Query: 121 TTAGGVIHKYLRSITLNHFGSESIKEKLLPDIQRYVNKVLHQWSKQPSVEVQRGTLAMLY 180
           TTAGGVIHKYLR ITLNHFGSES+K KLL DIQR V+KVL QWS +PSVE+Q GTLAMLY
Sbjct: 127 TTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHGTLAMLY 186

Query: 181 DFNAEKMFGYDAEKSSENISDSLITLADGFMAFPLNIPGTKYHKCLKAQKKLVNMLKGLV 240
           DFNA+ MFGYD EKSSE+IS+SLITLADGFMA PLN+PGTKY+KCLKAQKKLVNM K LV
Sbjct: 187 DFNADVMFGYDPEKSSESISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVNMFKDLV 246

Query: 241 KERRQANSTAARGDFLDQALRDIDNEQFLTEEFVVNLLFGILFASGSVSGSLTLMFKLLA 300
           KERRQA +  ARGDFLDQALRDI +EQFL+EEFVVNLLFGILFASGSVSGSLTLM KLLA
Sbjct: 247 KERRQAAAANARGDFLDQALRDIGSEQFLSEEFVVNLLFGILFASGSVSGSLTLMLKLLA 306

Query: 301 DNPSVVRELTAEHEAFLKQRKDPQSPITWEEYKSMTFTIQVVNETLRLSNALPFLLRRTV 360
           +NPSVVRELTAEHEAFLKQRKDP+SPITWEEYKSMTFT+QV+ ETLRLSNALPFLLRRT+
Sbjct: 307 ENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNALPFLLRRTI 366

Query: 361 KDVNIKGYTIPAGWTLMVANSALHLNPDTHKDPLAFNPWRWKEQDQYSISKNLQPFGGGA 420
           KD+NIKGYTIPAGWTLMVANSALHLNP THKDPL FNPWRWK+ DQ+SISKNLQPFGGG+
Sbjct: 367 KDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKNLQPFGGGS 426

Query: 421 RQCAGADYSRVFMAIFLHTLVTKYRWTKVKGGEICRSPILKFGDGIHISFSEKA 430
           RQCAGADYSRVFMAIFLHTL+TKY W KVKGG+I RSPILKFGDG+HISF  KA
Sbjct: 427 RQCAGADYSRVFMAIFLHTLLTKYSWRKVKGGDISRSPILKFGDGLHISFKAKA 480

BLAST of Sgr030490 vs. ExPASy TrEMBL
Match: A0A6J1KW76 (cytochrome P450 87A3-like OS=Cucurbita maxima OX=3661 GN=LOC111498129 PE=3 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 4.3e-205
Identity = 365/476 (76.68%), Postives = 397/476 (83.40%), Query Frame = 0

Query: 1   MWTPGLAIVAVAVICVTHLIYR-------------------------------------- 60
           MW+PGLAI AV VI +THL+YR                                      
Sbjct: 7   MWSPGLAIGAVVVILLTHLVYRWRNPKCNGLLPPGSMGFPLIGETIQLIASGNTLDLPPF 66

Query: 61  -------YGPIFRTSLLGRPIVVTADPEINNFIYQQEGRSVELWYLDSISKVFKQDGEAR 120
                  YGPIFRTSL+GR IVVTADPEIN+FIY QEG++VELWYLDSISKV KQDG+ R
Sbjct: 67  IKKRVQKYGPIFRTSLVGRSIVVTADPEINSFIYAQEGKTVELWYLDSISKVLKQDGDTR 126

Query: 121 TTAGGVIHKYLRSITLNHFGSESIKEKLLPDIQRYVNKVLHQWSKQPSVEVQRGTLAMLY 180
           TTAGGVIHKYLR ITLNHFGSES+K KLL DIQR V+KVL QWS +PSVE+Q GTLAMLY
Sbjct: 127 TTAGGVIHKYLRGITLNHFGSESLKTKLLADIQRCVDKVLVQWSSRPSVEMQHGTLAMLY 186

Query: 181 DFNAEKMFGYDAEKSSENISDSLITLADGFMAFPLNIPGTKYHKCLKAQKKLVNMLKGLV 240
           DFNA+ MFGYD EKSSENIS+SLITLADGFMA PLN+PGTKY+KCLKAQKKLVNM K LV
Sbjct: 187 DFNADVMFGYDPEKSSENISESLITLADGFMAVPLNLPGTKYNKCLKAQKKLVNMFKDLV 246

Query: 241 KERRQANSTA--ARGDFLDQALRDIDNEQFLTEEFVVNLLFGILFASGSVSGSLTLMFKL 300
           KERRQA + A  A+GDFLDQALRDI +EQFL+EEFVVNLLFGILFASGSVSGSLTLM KL
Sbjct: 247 KERRQAAAAAANAQGDFLDQALRDIGSEQFLSEEFVVNLLFGILFASGSVSGSLTLMLKL 306

Query: 301 LADNPSVVRELTAEHEAFLKQRKDPQSPITWEEYKSMTFTIQVVNETLRLSNALPFLLRR 360
           LA+NPSVVRELTAEHEAFLKQRKDP+SPITWEEYKSMTFT+QV+ ETLRLSNALPFLLRR
Sbjct: 307 LAENPSVVRELTAEHEAFLKQRKDPESPITWEEYKSMTFTLQVIYETLRLSNALPFLLRR 366

Query: 361 TVKDVNIKGYTIPAGWTLMVANSALHLNPDTHKDPLAFNPWRWKEQDQYSISKNLQPFGG 420
           T+KD+NIKGYTIPAGWTLMVANSALHLNP THKDPL FNPWRWK+ DQ+SISKNLQPFGG
Sbjct: 367 TIKDLNIKGYTIPAGWTLMVANSALHLNPGTHKDPLDFNPWRWKDLDQHSISKNLQPFGG 426

Query: 421 GARQCAGADYSRVFMAIFLHTLVTKYRWTKVKGGEICRSPILKFGDGIHISFSEKA 430
           G+RQCAGADYSRVFMAIFLHTL+TKY W KVKGG+I RSPILKFGDG+HISF  KA
Sbjct: 427 GSRQCAGADYSRVFMAIFLHTLLTKYSWRKVKGGDISRSPILKFGDGLHISFKAKA 482

BLAST of Sgr030490 vs. TAIR 10
Match: AT1G12740.2 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 394.4 bits (1012), Expect = 1.1e-109
Identity = 182/409 (44.50%), Postives = 275/409 (67.24%), Query Frame = 0

Query: 22  RYGPIFRTSLLGRPIVVTADPEINNFIYQQEGRSVELWYLDSISKVFKQDGEARTTAGGV 81
           RYGPIF+T+L+GRP++V+ D +++ F++ QEGR  + WY D+ + +F +      +  G 
Sbjct: 72  RYGPIFKTNLVGRPVIVSTDADLSYFVFNQEGRCFQSWYPDTFTHIFGKKNVG--SLHGF 131

Query: 82  IHKYLRSITLNHFGSESIKEKLLPDIQRYVNKVLHQWSKQPSVEVQRGTLAMLYDFNAEK 141
           ++KYL+++ L  FG + +K K+LP ++   NK L  WS Q SVE++  T +M++D  A+K
Sbjct: 132 MYKYLKNMVLTLFGHDGLK-KMLPQVEMTANKRLELWSNQDSVELKDATASMIFDLTAKK 191

Query: 142 MFGYDAEKSSENISDSLITLADGFMAFPLNIPGTKYHKCLKAQKKLVNMLKGLVKERRQA 201
           +  +D +KSSEN+  + +    G ++FP +IPGT YHKCL+ + K + ML+ +++ERR+ 
Sbjct: 192 LISHDPDKSSENLRANFVAFIQGLISFPFDIPGTAYHKCLQGRAKAMKMLRNMLQERRE- 251

Query: 202 NSTAARGDFLDQALRDIDNE-QFLTEEFVVNLLFGILFAS-GSVSGSLTLMFKLLADNPS 261
           N      DF D  + +I  E   LTEE  ++L+F +LFAS  + S +LTL  K L+D+P 
Sbjct: 252 NPRKNPSDFFDYVIEEIQKEGTILTEEIALDLMFVLLFASFETTSLALTLAIKFLSDDPE 311

Query: 262 VVRELTAEHEAFLKQRKDPQSPITWEEYKSMTFTIQVVNETLRLSNALPFLLRRTVKDVN 321
           V++ LT EHE  L+ R+D  S +TWEEYKSMT+T Q +NET RL+N +P + R+ ++D+ 
Sbjct: 312 VLKRLTEEHETILRNREDADSGLTWEEYKSMTYTFQFINETARLANIVPAIFRKALRDIK 371

Query: 322 IKGYTIPAGWTLMVANSALHLNPDTHKDPLAFNPWRWKEQDQYSISKNLQPFGGGARQCA 381
            K YTIPAGW +MV   A+HLNP+ +KDPL FNP RW+     + SK+   FGGG R C 
Sbjct: 372 FKDYTIPAGWAVMVCPPAVHLNPEMYKDPLVFNPSRWEGSKVTNASKHFMAFGGGMRFCV 431

Query: 382 GADYSRVFMAIFLHTLVTKYRWTKVKGGEICRSPILKFGDGIHISFSEK 429
           G D++++ MA FLH+LVTKYRW ++KGG I R+P L+F +G H+   +K
Sbjct: 432 GTDFTKLQMAAFLHSLVTKYRWEEIKGGNITRTPGLQFPNGYHVKLHKK 476

BLAST of Sgr030490 vs. TAIR 10
Match: AT1G12740.1 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 393.3 bits (1009), Expect = 2.5e-109
Identity = 181/411 (44.04%), Postives = 276/411 (67.15%), Query Frame = 0

Query: 20  IYRYGPIFRTSLLGRPIVVTADPEINNFIYQQEGRSVELWYLDSISKVFKQDGEARTTAG 79
           + +YGPIF+T+L+GRP++V+ D +++ F++ QEGR  + WY D+ + +F +      +  
Sbjct: 64  VKKYGPIFKTNLVGRPVIVSTDADLSYFVFNQEGRCFQSWYPDTFTHIFGKKNVG--SLH 123

Query: 80  GVIHKYLRSITLNHFGSESIKEKLLPDIQRYVNKVLHQWSKQPSVEVQRGTLAMLYDFNA 139
           G ++KYL+++ L  FG + +K K+LP ++   NK L  WS Q SVE++  T +M++D  A
Sbjct: 124 GFMYKYLKNMVLTLFGHDGLK-KMLPQVEMTANKRLELWSNQDSVELKDATASMIFDLTA 183

Query: 140 EKMFGYDAEKSSENISDSLITLADGFMAFPLNIPGTKYHKCLKAQKKLVNMLKGLVKERR 199
           +K+  +D +KSSEN+  + +    G ++FP +IPGT YHKCL+ + K + ML+ +++ERR
Sbjct: 184 KKLISHDPDKSSENLRANFVAFIQGLISFPFDIPGTAYHKCLQGRAKAMKMLRNMLQERR 243

Query: 200 QANSTAARGDFLDQALRDIDNE-QFLTEEFVVNLLFGILFAS-GSVSGSLTLMFKLLADN 259
           + N      DF D  + +I  E   LTEE  ++L+F +LFAS  + S +LTL  K L+D+
Sbjct: 244 E-NPRKNPSDFFDYVIEEIQKEGTILTEEIALDLMFVLLFASFETTSLALTLAIKFLSDD 303

Query: 260 PSVVRELTAEHEAFLKQRKDPQSPITWEEYKSMTFTIQVVNETLRLSNALPFLLRRTVKD 319
           P V++ LT EHE  L+ R+D  S +TWEEYKSMT+T Q +NET RL+N +P + R+ ++D
Sbjct: 304 PEVLKRLTEEHETILRNREDADSGLTWEEYKSMTYTFQFINETARLANIVPAIFRKALRD 363

Query: 320 VNIKGYTIPAGWTLMVANSALHLNPDTHKDPLAFNPWRWKEQDQYSISKNLQPFGGGARQ 379
           +  K YTIPAGW +MV   A+HLNP+ +KDPL FNP RW+     + SK+   FGGG R 
Sbjct: 364 IKFKDYTIPAGWAVMVCPPAVHLNPEMYKDPLVFNPSRWEGSKVTNASKHFMAFGGGMRF 423

Query: 380 CAGADYSRVFMAIFLHTLVTKYRWTKVKGGEICRSPILKFGDGIHISFSEK 429
           C G D++++ MA FLH+LVTKYRW ++KGG I R+P L+F +G H+   +K
Sbjct: 424 CVGTDFTKLQMAAFLHSLVTKYRWEEIKGGNITRTPGLQFPNGYHVKLHKK 470

BLAST of Sgr030490 vs. TAIR 10
Match: AT1G78490.1 (cytochrome P450, family 708, subfamily A, polypeptide 3 )

HSP 1 Score: 265.0 bits (676), Expect = 1.0e-70
Identity = 146/410 (35.61%), Postives = 234/410 (57.07%), Query Frame = 0

Query: 22  RYGPIFRTSLLGRPIVVTADPEINNFIYQQEGRSVELWYLDSISKVFKQDGEARTTAGGV 81
           RYGP+FRT++ G   VV+ DP++ + I++QE  S EL Y D   KVF +D          
Sbjct: 70  RYGPLFRTNIFGSKTVVSTDPDVIHQIFRQENTSFELGYPDIFVKVFGKDNLFLKEV--F 129

Query: 82  IHKYLRSITLNHFGSESIKEKLLPDIQRYVNKVLHQWSKQPSVEVQRGTLAMLYDFNAEK 141
           IHKYL+ IT+   GSE +K+ +L ++ +     +   + Q S  V++    ++  +   K
Sbjct: 130 IHKYLQKITMQILGSEGLKQTMLGNMDKATRDHIRSIASQGSFNVRKEVENLVVAYMTPK 189

Query: 142 MFGYDAEKSSENISDSLITL-ADGFMAFPLNIPGTKYHKCLKAQKKLVNMLKGLVKERRQ 201
           +      ++   + D+L     D F +F          K LK++++ + ++K ++  R++
Sbjct: 190 LISNLKPETQSKLIDNLNAFNLDWFKSFLRLSTWKAVTKALKSREEAIQVMKDVLMMRKE 249

Query: 202 ANSTAARGDFLDQALRDIDNE-QFLTEEFVVNLLFGILFASGSVSGSLT-LMFKLLADNP 261
                 + DFL+  L +++ +  F  +   +NL+F + FA    + S T L  K ++ +P
Sbjct: 250 TRE--KQEDFLNTLLEELEKDGSFFDQGSAINLIFLLAFALREGTSSCTALAVKFISKDP 309

Query: 262 SVVRELTAEHEAFLKQRKDPQSPITWEEYK-SMTFTIQVVNETLRLSNALPFLLRRTVKD 321
            V+ EL  EH+A +  RKD ++ ++WEEY+ +MTFT  V NE LRL+N  P L R+ V+D
Sbjct: 310 KVLAELKREHKAIVDNRKDKEAGVSWEEYRHNMTFTNMVSNEVLRLANTTPLLFRKAVQD 369

Query: 322 VNIKGYTIPAGWTLMVANSALHLNPDTHKDPLAFNPWRWKEQDQYSISKNLQPFGGGARQ 381
           V IKGYTIPAGW + VA SA+H +P  +++P  FNPWRW+ ++    SK    FG G R 
Sbjct: 370 VEIKGYTIPAGWIVAVAPSAVHFDPAIYENPFEFNPWRWEGKEMIWGSKTFMAFGYGVRL 429

Query: 382 CAGADYSRVFMAIFLHTLVTKYRWTKVKGGEICRSPILKFGDGIHISFSE 428
           C GA++SR+ MAIFLH LV  Y ++ V+  EI RSP  ++   + I+ S+
Sbjct: 430 CVGAEFSRLQMAIFLHHLVAYYDFSMVQDSEIIRSPFHQYTKDLLINISQ 475

BLAST of Sgr030490 vs. TAIR 10
Match: AT1G55940.1 (cytochrome P450, family 708, subfamily A, polypeptide 1 )

HSP 1 Score: 262.3 bits (669), Expect = 6.8e-70
Identity = 156/429 (36.36%), Postives = 243/429 (56.64%), Query Frame = 0

Query: 24  GPIFRTSLLGRPIVVTADPEINNFIYQQEGRSVELWYLDSISKVFKQDGEARTTAGGVIH 83
           G +FRT++LG   +V+ DPE+N  I +QE R   + Y +++ ++F +D       G   H
Sbjct: 218 GSLFRTNILGSKTIVSTDPEVNFEILKQENRCFIMSYPEALVRIFGKD-NLFFKQGKDFH 277

Query: 84  KYLRSITLNHFGSESIKEKLLPDIQRYVNKVLHQWSKQPSVEV---------QRGTLAML 143
           +Y+R I L   G E +K++ +  I    ++ L   S Q  V+V         ++  L ++
Sbjct: 278 RYMRHIALQLLGPECLKQRFIQQIDIATSEHLKSVSFQGVVDVKDTSGRLILEQMILMII 337

Query: 144 YDFNAE---------KMFGYDAEKSSENISDSLITLADGFMAFP--LNIPGTKYHKCLKA 203
            +   E         + F +D  +S  + S     L +G M F   LNI    ++  +KA
Sbjct: 338 SNIKPETKSKLIESFRDFSFDLVRSPFDPS-FWNALYNGLMDFVWLLNILDPLFY--VKA 397

Query: 204 QKKLVNMLKGLVKERRQ--ANSTAARGDFLDQALRDIDNE-QFLTEEFVVNLLFGILFAS 263
           +  ++ MLK + KERR+   +  +  GDF++  + +++ E   + EE  V L+  +L AS
Sbjct: 398 RSNVMKMLKRMFKERREEATSDDSKYGDFMETMIYEVEKEGDTINEERSVELILSLLIAS 457

Query: 264 GSVSGSLT-LMFKLLADNPSVVRELTAEHEAFLKQRKDPQSPITWEEYKS-MTFTIQVVN 323
              + ++T L  K +A+NP V+ EL  EHE  L+ R D +S +TW+EY+S M FT  V+N
Sbjct: 458 YETTSTMTALTVKFIAENPKVLMELKREHETILQNRADKESGVTWKEYRSMMNFTHMVIN 517

Query: 324 ETLRLSNALPFLLRRTVKDVNIKGYTIPAGWTLMVANSALHLNPDTHKDPLAFNPWRWKE 383
           E+LRL +  P + R+ V DV IKGYTIPAGW ++V  S LH +P  ++ P  FNPWRW+ 
Sbjct: 518 ESLRLGSLSPAMFRKAVNDVEIKGYTIPAGWIVLVVPSLLHYDPQIYEQPCEFNPWRWEG 577

Query: 384 QDQYSISKNLQPFGGGARQCAGADYSRVFMAIFLHTLVTKYRWTKVKGGEICRSPILKFG 428
           ++  S SK    FGGGAR CAGA+++R+ MAIFLH LVT Y ++ +    I R+P+L+F 
Sbjct: 578 KELLSGSKTFMAFGGGARLCAGAEFARLQMAIFLHHLVTTYDFSLIDKSYIIRAPLLRFS 637

BLAST of Sgr030490 vs. TAIR 10
Match: AT5G48000.2 (cytochrome P450, family 708, subfamily A, polypeptide 2 )

HSP 1 Score: 260.8 bits (665), Expect = 2.0e-69
Identity = 141/410 (34.39%), Postives = 230/410 (56.10%), Query Frame = 0

Query: 22  RYGPIFRTSLLGRPIVVTADPEINNFIYQQEGRSVELWYLDSISKVFKQDGEARTTAGGV 81
           +YGP+FRT++ G   VV  +P+I   +++QE +S    Y ++  K F +  E      G 
Sbjct: 70  KYGPLFRTNIFGSNTVVLTEPDIIFEVFRQENKSFVFSYPEAFVKPFGK--ENVFLKHGN 129

Query: 82  IHKYLRSITLNHFGSESIKEKLLPDIQRYVNKVLHQWSKQPSVEVQRGTLAMLYDFNAEK 141
           IHK+++ I+L H GSE++K+K++ +I R   + L   + Q S + +    +++      K
Sbjct: 130 IHKHVKQISLQHLGSEALKKKMIGEIDRVTYEHLRSKANQGSFDAKEAVESVIMAHLTPK 189

Query: 142 MFGYDAEKSSENISDSLITLADGFMAFPLNIPG-TKYHKCLKAQKKLVNMLKGLVKERRQ 201
           +      ++   + D+++ L   +   PL +      +K   A++  + ++K +   R+ 
Sbjct: 190 IISNLKPETQATLVDNIMALGSEWFQSPLKLTTLISIYKVFIARRYALQVIKDVFTRRKA 249

Query: 202 ANSTAARGDFLDQALRDIDNEQFL-TEEFVVNLLFGIL-FASGSVSGSLTLMFKLLADNP 261
           +      GDFLD  + + + E  +  EE  +NL+F IL  A  S S   +L  K LA+N 
Sbjct: 250 SREMC--GDFLDTMVEEGEKEDVIFNEESAINLIFAILVVAKESTSSVTSLAIKFLAENH 309

Query: 262 SVVRELTAEHEAFLKQRKDPQSPITWEEYK-SMTFTIQVVNETLRLSNALPFLLRRTVKD 321
             + EL  EH A L+ R    + ++WEEY+  MTFT  V+NETLR++N  P + R+ V D
Sbjct: 310 KALAELKREHAAILQNRNGKGAGVSWEEYRHQMTFTNMVINETLRMANMAPIMYRKAVND 369

Query: 322 VNIKGYTIPAGWTLMVANSALHLNPDTHKDPLAFNPWRWKEQDQYSISKNLQPFGGGARQ 381
           V IKGYTIPAGW + V   A+H N   +++PL FNPWRW+ ++  S SK    FGGG RQ
Sbjct: 370 VEIKGYTIPAGWIVAVIPPAVHFNDAIYENPLEFNPWRWEGKELRSGSKTFMVFGGGVRQ 429

Query: 382 CAGADYSRVFMAIFLHTLVTKYRWTKVKGGEICRSPILKFGDGIHISFSE 428
           C GA+++R+ ++IF+H LVT Y ++  +  E  R+P+  F  G+ I  S+
Sbjct: 430 CVGAEFARLQISIFIHHLVTTYDFSLAQESEFIRAPLPYFPKGLPIKISQ 475