Sgr030463 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr030463
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionMethionine--tRNA ligase
Locationtig00153654: 1585456 .. 1592791 (-)
RNA-Seq ExpressionSgr030463
SyntenySgr030463
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCTATGTTGATGGATATTTCTACTTAAGTTGTAGATTTCAAATTCACCTCTATAGTTATGACGTAATATTTAAGAAAAAAACTAAATTTATTGACATTTTTTTACATTTTGTCGATATTTTTATGTATAATGATTTCATATCGATTGATCCTTTTTTTAGGTGTCGAATCATTGGACATAAGCATGTGAATGAAAATCTTCCCCCACCCGTCTTCTCTCTTCTTAACCTTCATTCTCTTCCCAGGTCTAATCAGTTCTAACCAAACCCGAACCGAATCGATCCGGTTCGGTTCGGGTTTCATCCAAAGAGCAGTTCGGTTCGGTTCCAGAAATACAGATGGAGCCACCTCCATACAAAAAGTTCAGGTAGAAGAAACACCTTCGTCCGTTGGCTCTGGCTGCGTACACGCAGTGAAAGCCATTGAAATGGGCGCTAGAATAAACACCTCCATTCAAAGTTCACTCTGGCTCTTAAATCCTTTGCAGAATTTGAAAGCCACCCATCTCAGAACCCGCCTCCATTTCTCTCGAACTCTGTTCTCCTCCCCTTCTTCTTCTTCTTCTTCTTCTTCTTCTCCTTCATCAAAACGAGCTATATTCTGTACCTCTGCAAGCAATTCCAATTCCCATGAAAGAGAACCCGCAGCAGACCCCTTTGTCATCACAACACCACTTTATTATGTGAATGCTGCCCCTCATATGGGGAGTGCTTACACCACTATCGCCGCTGATGCCATTGCCCGATTTCAGGTACACCTCTATTTCTTAATCTAAAATCTTCATACTTGTAGTTCTATTTCACTGTTCGTTTAGTAATTGGTCTTACAGCTCTTCTTATTTGCTGTTTTTTCACTTGGGAAACTAGTGGGGGCGTTTGTTTAATGCTTGCTTTCAATTGGCTCTGAATAATTCTCCTCTGTTATTGGGTAAATTGGAGACACTGCCATCATGTTTTCTTTGTATGCTCCATTACCTTCCAGGCAATATTTGAAAGCAAGAACGTCCAAGGATTCAATTCCACACACCTCTCACTTGTTTTAGACTTTTAATATTTGAAATTTGGTATACAAATTTACCACTGATCACTCCTTGTTGAATTTAAAATTTTCAGTCTCTACCTTTCCGAATGGGAGTGTGTATCAAGATGTTCCAATTTCAAGATATCTTTTGAGTGGTGGACTAGCATTAATTTACTTACATTCAGTTGGATAATAATTTTGTTGTGTTCTGTGTTTTTAAAAATTAAACTTGTTTACTAACCATTACTTTATTTTGTAATCTACTTTTTTAAAACGTCTGTACGATTTTAGCCAAATTTTGAAGGCTAATTTTTAAAAGAAAAAACAAAAAACACAATTGTTATCAAATAGGCTTTAACTATTTGTTTTGTAGTTGGTTTCATTTAAAAAAACAGAACTTTAAGTACAAAGTAAAAGAATATATCAATTGTGCCGATCAAAACCAGTACTCCCTAGTTACTATTGCAGTTTTCCTTTAAGAGATGTTATAATGGTCAATAAAGTTTTGGTATGCCCCTTATATTGTCGATTTCTCGTACAGCTTACTAGTTGGGATACACTCTGTTGTCTGATTAGAGCTATTTCACCATGCTTATCTAGTTTCCACAGAATATTAAGTTATTATTTGGCTAACACCTTGTGTCATTTATAATAATAGAGGCTGCTGGGGAAGAAAGTTATATTCATTACTGGCACAGATGAGCATGGTGAGAAGATTGCTACAGCTGCTGCTGCTCAAGGTTCTGACCCAAGTGAACATTGTGATGCCATTTCAGAAGCATACAAGTCACTCTGGAAAGATGTAGTCCAAACACATCCGAGGATTTTCTTCTCAGTTTGCTGTAATAAGTTTATTAAATATTAGTCCTTATGTTTTCTTTTTTTTTTTTTCTTTTGGTTTGTTTCCCCTTTGCAGTTAGATGTAGCTTATGACAAGTTCATTCGGACAACTAATCCTAAACATGAGGCGATTGTAAAGGAATTTTATTCAAAGGTTCTTGCCAATGGCGATATTTATAGAGCTGATTATGAAGGACTTTATTGTGTCAACTGTGAAGAATACAAGGTATTTTTTTTTCTTTTTTTTCATGAATTTGAAAATATTATTTAATTTTTTGTCTTTCTCCCTCTACCCAGTTGAACATTTCGGATTCATATGATATTTACGCAACAAATTTCATAATTATTATTATCAATTGCTTCTTTCCTCAATTATGATAAAGTATTGATCTTTTGCTTTTCCGAGATTTATTTTTCCTGGGGTATGGATGCATTTAACAGTAGCTATTTCTTCAATTTTTATGGAATAGTCTAAATTACTTTTCTTGAATTTTATAAGCTCATCCATTTATAAACCTGCAGGACGAAAAAGAACTTCTTGCCAACAATTGTTGTCCTATACACCTAAAGCCCTGTGCTTCAAGGAAAGAAAACAATTACTTTTTTGCACTGTCAAAATATCAAAGATCGCTTGAAGAAACTTTATTGCAGAATCCTGGTTTTGTGCAGCCCGCCTTCCGTTTGAATGAGGTGACTATAAGGGATTATTCTACTTGTCTGAAATTTATCACTCCTCTTGTGTGTTTGTATGTGAAACTTATTAGCAAATTTATTGAATTTGTTCAAAATTGAAGAAACCCTGCATATAAGTGTGCGCGCACACCACTCCGAAGAGTTTTCCACTAGAGGCTACTACTTAGGAAGTCTAACCTTAAGATTGCTTATTTATTTATTTTTAAATGAGAAACTAACTTTCATTAATAGATATGAAAGTACAAAAAGCTAAGGGCCAAAATACAAGCCCAAACCAACTGCCGCGAAATTATAGAAAGCATCTTCAATTGTTAATGATTGAAAATAAAAGATAATCACGAAACTTTCTGTTCACAGACACCCAAAGGGGAGCATTGAAGTGAGCAATGTTTCAAACCTCCTCCCAAGCACCCTCTACACCCTAAAAATCCTCCTTTTCCTCTAGAATCACATAGAAACTGACTTGCCACAAAAGTATCCCTCCCTCCCGAAAAGGGGATACAGTCTAACCTTAAGATGAAAGCTATTTAAGTTCTGCTGGTTAGACATTCAATTACCTGTAATCTGATTGGATGCATTAACTTGCAACAAGTTTAAATTCATAGGAAATTGCAATAGAAATTTAAGATCAGCTATCTCATGTTTTAGCGAGGGAAGAATGCTGTAGATTTAAATCTGAGAATACTAGATTAGGCTGCCGTTGGAATTAATTTAATCTGGTTCACTAGAAAAATGAGCTAGAATCTCTTTCTGCTCACGCTCATGGTACAACTTATATGTGTGTGTGCATACACATTTGATAAGTAGTTACAAAAGGGGGAATAAGAGGGTTAGGCTGATAGGAAGCTCTTCCTAAAGTTAAAACTTTTTTTTTCTTTAAGATGCTCATTGAGCTATTGGATCAGACTATTTTCGTGTTTTCGAGAGGCTGGGTCTCTGCCATTGAAGCCCTTGGAGCTCTCTAATACCTTCCCTCACAATTTTCCTCTCATATAATAACTTTTGCTAACTGTTTGATTAACCTCTTAGCCCAATAACTTGTAACCAAGCGACTAACCTTTAGCCTTCCTTGGATATTTAAAATATGGTGGTGATCTAGCAACTCTATGGGAGGTGTCCACTTTTAACCAATGGCTTGGAGCTTACAAAAAGGCATTCTAATCAACTAAAATTTAAAACAATGGAAGGCACAGAAAAACCTATTAATAGAAGCTCAAAAAGAAAGCTGACAAACAATGTGATTCCCAAACCTCCCTTATTGTGATAGGATTTTCATAATTATATGAATAAACAAGGTGACCTATTGAATGATCTTGTGTAGTTGTAGAGTGGCTTACGAAGAGCGTTGATACAAAACTCCATGCACATATATTATAAATTATATTCAAGGTCTACCATTATGTATGACCTAGTCCTAAACTAAACGTGAAAGCCTTGTAATTGCTCATCATATCTCTTTGATCTTATTTGATGTTGTACTCATATAGCCATTCTTCTGACAACAGAATTGTCAGTTCTATTGTTTGTTACGATTTTTTCCACCTTGCGGATCTTGAATCATCCTTTGATTAATTCAGCACCATATCTTCCAAGGTGCAAAGTTGGATTAGAAGTGGCCTAAAAGACTTCTCTATCTCCAGAGCGTCAGTTGAATGGGGCATTCCAGTTCCAAATGATCAAAAGCAAACAATTTATGTTTGGTTTGATGCATTATTAGGGTAATCTCTTCCTTATCTTTTTGTAATGTCTTTTATACTGTGATTTCTTGAAACTCTATGATTCTCGCCCACCTTTTTTTTCTTTTAAATAATAAAGAAAGAATTCTTCATTACTACAAACATGATTTATGTAAACTTGCAACAGCTCTCCACCGTTTTGTTTAGGAAATTGAAATGGAAAGTAAGTCAGTGCCTTCAATCTGAATCATATGAGAATTGCATAATGTAGATATATATCAGCACTATCAGAGAACGGTGAGCAACCAAAGTTACAAAGGATTGTTGCTTCAGGTTGGCCTGCCTCTTTACACTTGATTGGTAAGGTAAGAGATACATTCCTGTTCTTCAATGATATTTTAGGGCAAATTTCCTGATTGTACATAGTCTCATCTAGCCCTAGCTGGAAAACCTTTTTCTTTTCACCTGTTTTTCAGATTTAAGTATTTGCTCTATTTTTCTTGAATATAGCGAGAATTGAATCTATTCATGAGCTCCAATTTCTGTGTTATTTTGTAGTCTGGAGGTTCTGATGATTTTATCACTTTTCTTTTCAATACTGAGTGCAAGCTCGGTATGTAGGATGCAGATCTAATGTTTTTGTTATTCTGTTTGTAGGATATTTTACGTTTCCATGCAGTTTATTGGCCTGCTATGCTAATGTCTGCGGGACTAAAACTTCCAAAGATGGTTTTTGGTCATGGATTCCTGACAAAGGTATGTTTGGCCTCCCAAAGTTGCTACAGACAGGAATTAGTTGGCCATGGTAATGGTCTAATAATGCAGAAGAAAGTAAATATTTACTATTTAGGAGGTCTATAAAGATGTAACCTGTCTCCATTGCTTTTATATATAATGTGAATATTGTTGCATCTTGTGAATTCTAAAGGGTCTTGATTGTCTTGTGAATCTTACTGAATCTCTTGGAATTGGTCAACATTCAATGCTCCTTCTAGCCAAATATTTCCAACAATTTGATGCTGCCAGGATTCATGATTTCTTAGCATTGTGTAGAATTAATTATTTGTATTGGAATTGTGAGCAATTGGTAGATTTAATACCTGTTATATTTTGCAATGGAGAAAACTCGTTATTATCTACTTTTTGGTGAATTTTATTTCAATGTGTGAGAATTAAGCTATTACAATATGTTTCTTATTCATGCCTTGCAAAGATTTTCTGGTTCAAGCTTATTTATGTATTTGATGTATTTTGGTTCCTAAAAAATGTCCATTTGTGGGCAAAGCTAGTATTGATTCTAGAGAAGCTTGAAGATTTTAATCATGTTAGTGGGCTTGAAGTTGTTTGCTTCCAAAATCAATTTGAGTTCTGGTGGAATTCCTTGCGATTTTGTAGGTGGAATTTCTTGCAAATTTGTTAGTTTCTGGTGTAAAGAGTGGTGGTCTTTCTTTTCATATTTCTTAGCTGTCCATCTCTCTTGTAGTGAAAATATGACATTTCTTATAAATTGAATGCATGTGATCTCTCCCTAACTTCTTTCTGGTTCATGGTTCGAGTGGTAAAGAGTGAGTATTATCACAACTGAAGATTTTCTTACTAGAACACAGATTCATAAACCCCATGTATTCTATGAATTTGGCATCGTAATCTTGTTTGTAAACATTTCTTTCTGGAATTTTTAAGTCCTGGCCTTCTTTTGTCTGTTCTAAAATTGTTGGTCAAGTTGCAAAACCTCTCATTACTAATTTTATTTCATGCAGCAACTCTTTTTGTTTTAACTTTATAGGATGGCATGAAGATGGGCAAGTCATTAGGCAATACCCTTGAACCAAATGAATTGGTTCAAAAGTTTGGCTCTGATGCAGTGAGATACTTCTTCCTTCGGGAGGTGGAATTTGGTAGCGACGGAGACTACGCTGAGGATCGTTTCATCAATATTGTCAATGCACATCTTGCAAATACAATAGGTATTCATAAACCATAGTGATAATTCCTTTCAAACGGAAATTACAGCCCTTTTACTCGAGACTTCTGTTTGACAACATAATTCTTGACATTTCTCTTGTAGGAAATCTGCTTAACCGAACTCTCGGACTCCTTAAGAAGAACTGCCAATCAACTTTGGTGGTTGATTCTACTGCTGCAGCTGAAGGAACTGCATTTAAGGACAAAGTTGAAGAGCTGGTAACATCTGGTTGGACTTTATATTGTTATTTGTCATCTGAGATGGCATGGAATTAGTGAAATGATTGCACATCTAACTTGTAGACCTTGAAACCTTCTCTATAATCACTTTTGTAAGTGGCATCTCAGTGGATGGTGATGGTGCATTAAACTTTATGTATGTGCTTGGTTTCTGAATCTATTGTGTTGGGGGATATTGCCTGTTGTGTGTCCTCTATTACGGACTTTGTGACCAAACATGTTCACGACTTGTGTGAACTGTGTTAATTTTATTCGTATGCTTGTAGGTTGTGAAAGCTCGGAATCATTATGAAGAACTCTCCCTTTCATCGGCTTGTGAGGCTGTGTTGGAGATTGGTAATGCAGGAAATTCCTACATAGATGAGCGAGCACCATGGTCTCTATTCAAGCAAGGTGGTGCCTCTTCAGCAGCCGCTGCAAAGGTTGTTGTTTTGTTCAAGAACCCATTTGACGTTATCTGATGATTGACTGACTATGCCTTCTAACCACACAAGTTTCTTGGTTATGGCATCAAATTCTGTTTAATTAATAAAATCATTCTCTGACAGCTATCTCGGCTTGATTTTCTCGACAGGACCTCATACTTATACTCGAGGCGATGCGAATCATAGCAGTTGCACTATCCCCAGTGACACCATGCTTAAGCTGGAGAATATATGCCCAGCTCGGCTTCTCGAAGGACCAGTTTGAAGCTGCCACATGGGTCTCTCTCTCTCTCTCTCTCACTGGTGTTGTGTTCTGTGTGTATTTTTATATCAGCTCTTTTTATTAGAACGGACTGATAATATGGTTTTTGGATGTAGAATGAGACAAAATGGGGAGGGCTGAAGGCTGGTCAGGTCATGGCTCCTCCAAAACCAGTCTTTGCAAGGATAGAACAAGCCTCTCTTAGAAGATGA

mRNA sequence

ATGAGCTATGTTGATGGATATTTCTACTTAAGTTGTCTAATCAGTTCTAACCAAACCCGAACCGAATCGATCCGGTTCGGTTCGGGTTTCATCCAAAGAGCAGTTCGGTTCGGTTCCAGAAATACAGATGGAGCCACCTCCATACAAAAAGTTCAGGTAGAAGAAACACCTTCGTCCGTTGGCTCTGGCTGCGTACACGCAGTGAAAGCCATTGAAATGGGCGCTAGAATAAACACCTCCATTCAAAGTTCACTCTGGCTCTTAAATCCTTTGCAGAATTTGAAAGCCACCCATCTCAGAACCCGCCTCCATTTCTCTCGAACTCTGTTCTCCTCCCCTTCTTCTTCTTCTTCTTCTTCTTCTTCTCCTTCATCAAAACGAGCTATATTCTGTACCTCTGCAAGCAATTCCAATTCCCATGAAAGAGAACCCGCAGCAGACCCCTTTGTCATCACAACACCACTTTATTATGTGAATGCTGCCCCTCATATGGGGAGTGCTTACACCACTATCGCCGCTGATGCCATTGCCCGATTTCAGAGGCTGCTGGGGAAGAAAGTTATATTCATTACTGGCACAGATGAGCATGGTGAGAAGATTGCTACAGCTGCTGCTGCTCAAGGTTCTGACCCAAGTGAACATTGTGATGCCATTTCAGAAGCATACAAGTCACTCTGGAAAGATTTAGATGTAGCTTATGACAAGTTCATTCGGACAACTAATCCTAAACATGAGGCGATTGTAAAGGAATTTTATTCAAAGGTTCTTGCCAATGGCGATATTTATAGAGCTGATTATGAAGGACTTTATTGTGTCAACTGTGAAGAATACAAGGACGAAAAAGAACTTCTTGCCAACAATTGTTGTCCTATACACCTAAAGCCCTGTGCTTCAAGGAAAGAAAACAATTACTTTTTTGCACTGTCAAAATATCAAAGATCGCTTGAAGAAACTTTATTGCAGAATCCTGGTTTTGTGCAGCCCGCCTTCCGTTTGAATGAGGTGCAAAGTTGGATTAGAAGTGGCCTAAAAGACTTCTCTATCTCCAGAGCGTCAGTTGAATGGGGCATTCCAGTTCCAAATGATCAAAAGCAAACAATTTATGTTTGGTTTGATGCATTATTAGGATATATATCAGCACTATCAGAGAACGGTGAGCAACCAAAGTTACAAAGGATTGTTGCTTCAGGTTGGCCTGCCTCTTTACACTTGATTGGTAAGGATATTTTACGTTTCCATGCAGTTTATTGGCCTGCTATGCTAATGTCTGCGGGACTAAAACTTCCAAAGATGGTTTTTGGTCATGGATTCCTGACAAAGGATGGCATGAAGATGGGCAAGTCATTAGGCAATACCCTTGAACCAAATGAATTGGTTCAAAAGTTTGGCTCTGATGCAGTGAGATACTTCTTCCTTCGGGAGGTGGAATTTGGTAGCGACGGAGACTACGCTGAGGATCGTTTCATCAATATTGTCAATGCACATCTTGCAAATACAATAGGAAATCTGCTTAACCGAACTCTCGGACTCCTTAAGAAGAACTGCCAATCAACTTTGGTGGTTGATTCTACTGCTGCAGCTGAAGGAACTGCATTTAAGGACAAAGTTGAAGAGCTGGTTGTGAAAGCTCGGAATCATTATGAAGAACTCTCCCTTTCATCGGCTTGTGAGGCTGTGTTGGAGATTGGTAATGCAGGAAATTCCTACATAGATGAGCGAGCACCATGGTCTCTATTCAAGCAAGGTGGTGCCTCTTCAGCAGCCGCTGCAAAGGACCTCATACTTATACTCGAGGCGATGCGAATCATAGCAGTTGCACTATCCCCAGTGACACCATGCTTAAGCTGGAGAATATATGCCCAGCTCGGCTTCTCGAAGGACCAGTTTGAAGCTGCCACATGGAATGAGACAAAATGGGGAGGGCTGAAGGCTGGTCAGGTCATGGCTCCTCCAAAACCAGTCTTTGCAAGGATAGAACAAGCCTCTCTTAGAAGATGA

Coding sequence (CDS)

ATGAGCTATGTTGATGGATATTTCTACTTAAGTTGTCTAATCAGTTCTAACCAAACCCGAACCGAATCGATCCGGTTCGGTTCGGGTTTCATCCAAAGAGCAGTTCGGTTCGGTTCCAGAAATACAGATGGAGCCACCTCCATACAAAAAGTTCAGGTAGAAGAAACACCTTCGTCCGTTGGCTCTGGCTGCGTACACGCAGTGAAAGCCATTGAAATGGGCGCTAGAATAAACACCTCCATTCAAAGTTCACTCTGGCTCTTAAATCCTTTGCAGAATTTGAAAGCCACCCATCTCAGAACCCGCCTCCATTTCTCTCGAACTCTGTTCTCCTCCCCTTCTTCTTCTTCTTCTTCTTCTTCTTCTCCTTCATCAAAACGAGCTATATTCTGTACCTCTGCAAGCAATTCCAATTCCCATGAAAGAGAACCCGCAGCAGACCCCTTTGTCATCACAACACCACTTTATTATGTGAATGCTGCCCCTCATATGGGGAGTGCTTACACCACTATCGCCGCTGATGCCATTGCCCGATTTCAGAGGCTGCTGGGGAAGAAAGTTATATTCATTACTGGCACAGATGAGCATGGTGAGAAGATTGCTACAGCTGCTGCTGCTCAAGGTTCTGACCCAAGTGAACATTGTGATGCCATTTCAGAAGCATACAAGTCACTCTGGAAAGATTTAGATGTAGCTTATGACAAGTTCATTCGGACAACTAATCCTAAACATGAGGCGATTGTAAAGGAATTTTATTCAAAGGTTCTTGCCAATGGCGATATTTATAGAGCTGATTATGAAGGACTTTATTGTGTCAACTGTGAAGAATACAAGGACGAAAAAGAACTTCTTGCCAACAATTGTTGTCCTATACACCTAAAGCCCTGTGCTTCAAGGAAAGAAAACAATTACTTTTTTGCACTGTCAAAATATCAAAGATCGCTTGAAGAAACTTTATTGCAGAATCCTGGTTTTGTGCAGCCCGCCTTCCGTTTGAATGAGGTGCAAAGTTGGATTAGAAGTGGCCTAAAAGACTTCTCTATCTCCAGAGCGTCAGTTGAATGGGGCATTCCAGTTCCAAATGATCAAAAGCAAACAATTTATGTTTGGTTTGATGCATTATTAGGATATATATCAGCACTATCAGAGAACGGTGAGCAACCAAAGTTACAAAGGATTGTTGCTTCAGGTTGGCCTGCCTCTTTACACTTGATTGGTAAGGATATTTTACGTTTCCATGCAGTTTATTGGCCTGCTATGCTAATGTCTGCGGGACTAAAACTTCCAAAGATGGTTTTTGGTCATGGATTCCTGACAAAGGATGGCATGAAGATGGGCAAGTCATTAGGCAATACCCTTGAACCAAATGAATTGGTTCAAAAGTTTGGCTCTGATGCAGTGAGATACTTCTTCCTTCGGGAGGTGGAATTTGGTAGCGACGGAGACTACGCTGAGGATCGTTTCATCAATATTGTCAATGCACATCTTGCAAATACAATAGGAAATCTGCTTAACCGAACTCTCGGACTCCTTAAGAAGAACTGCCAATCAACTTTGGTGGTTGATTCTACTGCTGCAGCTGAAGGAACTGCATTTAAGGACAAAGTTGAAGAGCTGGTTGTGAAAGCTCGGAATCATTATGAAGAACTCTCCCTTTCATCGGCTTGTGAGGCTGTGTTGGAGATTGGTAATGCAGGAAATTCCTACATAGATGAGCGAGCACCATGGTCTCTATTCAAGCAAGGTGGTGCCTCTTCAGCAGCCGCTGCAAAGGACCTCATACTTATACTCGAGGCGATGCGAATCATAGCAGTTGCACTATCCCCAGTGACACCATGCTTAAGCTGGAGAATATATGCCCAGCTCGGCTTCTCGAAGGACCAGTTTGAAGCTGCCACATGGAATGAGACAAAATGGGGAGGGCTGAAGGCTGGTCAGGTCATGGCTCCTCCAAAACCAGTCTTTGCAAGGATAGAACAAGCCTCTCTTAGAAGATGA

Protein sequence

MSYVDGYFYLSCLISSNQTRTESIRFGSGFIQRAVRFGSRNTDGATSIQKVQVEETPSSVGSGCVHAVKAIEMGARINTSIQSSLWLLNPLQNLKATHLRTRLHFSRTLFSSPSSSSSSSSSPSSKRAIFCTSASNSNSHEREPAADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVKEFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFFALSKYQRSLEETLLQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEELSLSSACEAVLEIGNAGNSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSPVTPCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPKPVFARIEQASLRR
Homology
BLAST of Sgr030463 vs. NCBI nr
Match: XP_004142742.1 (methionine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN51284.1 hypothetical protein Csa_008274 [Cucumis sativus])

HSP 1 Score: 1072.0 bits (2771), Expect = 2.0e-309
Identity = 536/592 (90.54%), Postives = 555/592 (93.75%), Query Frame = 0

Query: 73  MGARINTSIQSSLWLLNPLQ---NLKATHLRTRLHFSRTLFSSPSSSSSSSSSPSSKRAI 132
           M ARINTSIQ+SLWLLNP     NLK TH RTRLHF + L S PS S       SSKRAI
Sbjct: 1   MAARINTSIQTSLWLLNPFHSSLNLKPTHFRTRLHFCQNLLSFPSHS-------SSKRAI 60

Query: 133 FCTSASNSNSHEREPAADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 192
           FCTS SN NSH+   A DPFVITTPLYYVNAAPHMGSAYTTIAADA+ARFQRLLGKKVIF
Sbjct: 61  FCTSTSNFNSHDSGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIF 120

Query: 193 ITGTDEHGEKIATAAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVK 252
           ITGTDEHGEKIATAAA QGS PSEHCD ISEAYK+LWKDLDVAYDKFIRTT+PKHEAIVK
Sbjct: 121 ITGTDEHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVK 180

Query: 253 EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFFALS 312
           EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKE+NYFFALS
Sbjct: 181 EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240

Query: 313 KYQRSLEETLLQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYV 372
           KYQR LEETL QNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND+KQTIYV
Sbjct: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYV 300

Query: 373 WFDALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 432
           WFDALLGYISALSENGEQP LQ +VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP
Sbjct: 301 WFDALLGYISALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360

Query: 433 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFI 492
           KMVFGHGFLTKDGMKMGKSLGNTLEPNELV+KFGSDAVRYFFLREVEFGSDGDYAEDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFI 420

Query: 493 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEE 552
           NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAA+G AFKDKVEELVVKARN+YEE
Sbjct: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEE 480

Query: 553 LSLSSACEAVLEIGNAGNSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSP 612
           L+LSSACEAVLEIGNAGNSY+DERAPWSLFKQGGASS AAAKDL+LILEAMRIIA+ALSP
Sbjct: 481 LALSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSP 540

Query: 613 VTPCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPKPVFARIEQA 662
           VTP LSWRIYAQLG+SKDQF+AATWNETKWGGLKAGQVMAPP PVFARIEQA
Sbjct: 541 VTPRLSWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQA 585

BLAST of Sgr030463 vs. NCBI nr
Match: XP_022133974.1 (methionine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia])

HSP 1 Score: 1068.5 bits (2762), Expect = 2.2e-308
Identity = 542/596 (90.94%), Postives = 558/596 (93.62%), Query Frame = 0

Query: 73  MGARINTSIQSSLWLLNPLQ-----NLKAT-HLRTRLHFSRTLFSSPSSSSSSSSSPSSK 132
           M ARIN+SIQSSLWLLNPLQ     NL    +LRTRL+F R L  SPS S+S     SSK
Sbjct: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSAS-----SSK 60

Query: 133 RAIFCTSASNSNSHEREPAADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKK 192
           RAIFCT ASN NSHE   A DPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKK
Sbjct: 61  RAIFCTYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKK 120

Query: 193 VIFITGTDEHGEKIATAAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEA 252
           VIFITGTDEHGEKIATAAAAQGS PSEHCDAISEAYK+LWKDLDVAYDKFIRTT+PKHEA
Sbjct: 121 VIFITGTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEA 180

Query: 253 IVKEFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFF 312
           IVKEFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKE+NYFF
Sbjct: 181 IVKEFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFF 240

Query: 313 ALSKYQRSLEETLLQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQT 372
           ALSKYQ  LEETL QNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQT
Sbjct: 241 ALSKYQGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQT 300

Query: 373 IYVWFDALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGL 432
           IYVWFDALLGYISALSENG+Q  LQR VASGWPASLHLIGKDILRFHAVYWPAMLMSAGL
Sbjct: 301 IYVWFDALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGL 360

Query: 433 KLPKMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAED 492
           KLPKMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFL+EVEFGSDGDYAED
Sbjct: 361 KLPKMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAED 420

Query: 493 RFINIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNH 552
           RFINIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEG +FKDKVEELV+KARNH
Sbjct: 421 RFINIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNH 480

Query: 553 YEELSLSSACEAVLEIGNAGNSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVA 612
           YE LSLSSACEAVLEIGNAGNSY+DE+APWSLFKQGGASS AAAKDL+LILEAMRIIAVA
Sbjct: 481 YEGLSLSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVA 540

Query: 613 LSPVTPCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPKPVFARIEQAS 663
           LSPVTPCL WRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPP PVFARIEQ++
Sbjct: 541 LSPVTPCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQST 591

BLAST of Sgr030463 vs. NCBI nr
Match: KAG7033041.1 (Methionine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1066.2 bits (2756), Expect = 1.1e-307
Identity = 536/592 (90.54%), Postives = 555/592 (93.75%), Query Frame = 0

Query: 73  MGARINTSIQSSLWLLNPLQ---NLKATHLRTRLHFSRTLFSSPSSSSSSSSSPSSKRAI 132
           M ARINTSIQ+SLWLLNPLQ   NLKATHLR R +F + L S+PSS SSS SS SSKRAI
Sbjct: 1   MAARINTSIQNSLWLLNPLQSSRNLKATHLRIRFNFYQNLLSAPSSCSSSCSSSSSKRAI 60

Query: 133 FCTSASNSNSHEREPAADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 192
           FC+SASN NSH+   A DPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF
Sbjct: 61  FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120

Query: 193 ITGTDEHGEKIATAAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVK 252
           ITGTDEHGEKIATAAAAQGS PSEHCD ISEAYK+LWKDLD+AYDKFIRTT+PKHE IVK
Sbjct: 121 ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK 180

Query: 253 EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFFALS 312
           EFYS VLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKE+NYFFALS
Sbjct: 181 EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240

Query: 313 KYQRSLEETLLQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYV 372
           KYQR LEETL QNPGFVQPAFRLNEVQSWI+SGLKDFSISRASVEWGIPVPNDQKQTIYV
Sbjct: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV 300

Query: 373 WFDALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 432
           WFDALLGYISALSEN EQP LQ  VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP
Sbjct: 301 WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360

Query: 433 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFI 492
           KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSD VRYFFLREVEFGSDGDYAEDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI 420

Query: 493 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEE 552
           NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDS+AAA G AFK+KVEELV KARNHYEE
Sbjct: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE 480

Query: 553 LSLSSACEAVLEIGNAGNSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSP 612
           LSLSSACEAVLEIGNAGNSY+DERAPWSLFKQGGASS AAAKDL+LILEAMRIIA+ALSP
Sbjct: 481 LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP 540

Query: 613 VTPCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPKPVFARIEQA 662
           VTP LSWRIY QLG+ +DQF AATWNETKWGGLKAGQVMAPP PVFARIEQA
Sbjct: 541 VTPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQA 592

BLAST of Sgr030463 vs. NCBI nr
Match: XP_038891037.1 (methionine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida])

HSP 1 Score: 1064.7 bits (2752), Expect = 3.3e-307
Identity = 531/592 (89.70%), Postives = 557/592 (94.09%), Query Frame = 0

Query: 73  MGARINTSIQSSLWLLNPLQ---NLKATHLRTRLHFSRTLFSSPSSSSSSSSSPSSKRAI 132
           M ARINTSIQ+SLWLLNP Q   NLK THLRTRLHF + L S PSSSS      SSKRAI
Sbjct: 1   MAARINTSIQTSLWLLNPCQSSLNLKPTHLRTRLHFYQNLLSLPSSSS------SSKRAI 60

Query: 133 FCTSASNSNSHEREPAADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 192
           FCT+A++ NSH+   A +PFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF
Sbjct: 61  FCTAATSFNSHDNGAATNPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120

Query: 193 ITGTDEHGEKIATAAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVK 252
           ITGTDEHGEKIATAAA QGS PSEHCD ISEAYK+LWKDLDVAYD+FIRTT+PKHEAIVK
Sbjct: 121 ITGTDEHGEKIATAAATQGSSPSEHCDVISEAYKTLWKDLDVAYDRFIRTTDPKHEAIVK 180

Query: 253 EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFFALS 312
           EFYS+VLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASR+E+NYFFALS
Sbjct: 181 EFYSRVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRREDNYFFALS 240

Query: 313 KYQRSLEETLLQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYV 372
           KYQR LEETL QNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND+KQTIYV
Sbjct: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYV 300

Query: 373 WFDALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 432
           WFDALLGYISALSENGEQP L+R+VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP
Sbjct: 301 WFDALLGYISALSENGEQPNLERVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360

Query: 433 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFI 492
           KMVFGHGFLTKDGMKMGKSLGNTLEPNELV KFGSDAVRYFFLREVEFGSDGDYAEDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVGKFGSDAVRYFFLREVEFGSDGDYAEDRFI 420

Query: 493 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEE 552
           NIVNAHLANTIGNLLNRTLGLL+KNCQSTLVVDSTAAA G  FKDKVEEL+VKARNHYEE
Sbjct: 421 NIVNAHLANTIGNLLNRTLGLLQKNCQSTLVVDSTAAAGGNVFKDKVEELIVKARNHYEE 480

Query: 553 LSLSSACEAVLEIGNAGNSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSP 612
           L+LSSACEAVLEIGNAGNSY+DERAPWSLFKQGGAS+ AAAKDL+LILEAMRIIA+ALSP
Sbjct: 481 LALSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASTDAAAKDLVLILEAMRIIAIALSP 540

Query: 613 VTPCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPKPVFARIEQA 662
           VTP LSWRIYAQLG+S+DQF AATWNETKWGGLKAGQVMAPP PVFARIEQA
Sbjct: 541 VTPRLSWRIYAQLGYSEDQFNAATWNETKWGGLKAGQVMAPPNPVFARIEQA 586

BLAST of Sgr030463 vs. NCBI nr
Match: XP_022960433.1 (methionine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata])

HSP 1 Score: 1062.4 bits (2746), Expect = 1.6e-306
Identity = 535/592 (90.37%), Postives = 554/592 (93.58%), Query Frame = 0

Query: 73  MGARINTSIQSSLWLLNPLQ---NLKATHLRTRLHFSRTLFSSPSSSSSSSSSPSSKRAI 132
           M ARINTSIQ+SLWLLNPLQ   NLKATHLRTR +F + L S+PSS SSS    SSKRAI
Sbjct: 1   MAARINTSIQNSLWLLNPLQSSRNLKATHLRTRFNFYQNLLSAPSSCSSS----SSKRAI 60

Query: 133 FCTSASNSNSHEREPAADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 192
           FC+SASN NSH+   A DPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF
Sbjct: 61  FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120

Query: 193 ITGTDEHGEKIATAAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVK 252
           ITGTDEHGEKIATAAAAQGS PSEHCD ISEAYK+LWKDLD+AYDKFIRTT+PKHE IVK
Sbjct: 121 ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK 180

Query: 253 EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFFALS 312
           EFYS VLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKE+NYFFALS
Sbjct: 181 EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240

Query: 313 KYQRSLEETLLQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYV 372
           KYQR LEETL QNPGFVQPAFRLNEVQSWI+SGLKDFSISRASVEWGIPVPNDQKQTIYV
Sbjct: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV 300

Query: 373 WFDALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 432
           WFDALLGYISALSEN EQP LQ  VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP
Sbjct: 301 WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360

Query: 433 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFI 492
           KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSD VRYFFLREVEFGSDGDYAEDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI 420

Query: 493 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEE 552
           NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDS+AAA G AFK+KVEELV KARNHYEE
Sbjct: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE 480

Query: 553 LSLSSACEAVLEIGNAGNSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSP 612
           LSLSSACEAVLEIGNAGNSY+DERAPWSLFKQGGASS AAAKDL+LILEAMRIIA+ALSP
Sbjct: 481 LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP 540

Query: 613 VTPCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPKPVFARIEQA 662
           VTP LSWRIY QLG+ +DQF AATWNETKWGGLKAGQVMAPP PVFARIEQA
Sbjct: 541 VTPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQA 588

BLAST of Sgr030463 vs. ExPASy Swiss-Prot
Match: Q9M2T9 (Methionine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OVA1 PE=2 SV=1)

HSP 1 Score: 890.2 bits (2299), Expect = 1.4e-257
Identity = 441/588 (75.00%), Postives = 500/588 (85.03%), Query Frame = 0

Query: 73  MGARINTSIQSSLWLLNPLQNLKATHLRTR-LHFSRTLFSSPSSSSSSSSSPSSKRAIFC 132
           M ARINTS+ ++L  L P      T L T+   F R  F         S   S KRA++C
Sbjct: 1   MAARINTSLHNALSFLKPFN----TPLNTKPFSFRRNSFRFSKKLPYYSQFSSGKRALYC 60

Query: 133 TSASNSNSHEREPAADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 192
           TS+S  ++ +     + FV+TTPLYYVNA PHMGSAYTTIAAD+IARFQRLLGKKVIFIT
Sbjct: 61  TSSSQESTVDE---GETFVLTTPLYYVNAPPHMGSAYTTIAADSIARFQRLLGKKVIFIT 120

Query: 193 GTDEHGEKIATAAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVKEF 252
           GTDEHGEKIAT+AAA G +P EHCD IS++Y++LWKDLD+AYDKFIRTT+PKHEAIVKEF
Sbjct: 121 GTDEHGEKIATSAAANGRNPPEHCDLISQSYRTLWKDLDIAYDKFIRTTDPKHEAIVKEF 180

Query: 253 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFFALSKY 312
           Y++V ANGDIYRADYEGLYCVNCEEYKDEKELL NNCCP+H  PC +RKE+NYFFALSKY
Sbjct: 181 YARVFANGDIYRADYEGLYCVNCEEYKDEKELLENNCCPVHQMPCVARKEDNYFFALSKY 240

Query: 313 QRSLEETLLQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 372
           Q+ LE+ L QNP FVQP++RLNEVQSWI+SGL+DFSISRA V+WGIPVP+D KQTIYVWF
Sbjct: 241 QKPLEDILAQNPRFVQPSYRLNEVQSWIKSGLRDFSISRALVDWGIPVPDDDKQTIYVWF 300

Query: 373 DALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 432
           DALLGYISAL+E+ +Q  L+  V+ GWPASLHLIGKDILRFHAVYWPAMLMSAGL+LPKM
Sbjct: 301 DALLGYISALTEDNKQQNLETAVSFGWPASLHLIGKDILRFHAVYWPAMLMSAGLELPKM 360

Query: 433 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINI 492
           VFGHGFLTKDGMKMGKSLGNTLEP ELVQKFG DAVRYFFLREVEFG+DGDY+EDRFI I
Sbjct: 361 VFGHGFLTKDGMKMGKSLGNTLEPFELVQKFGPDAVRYFFLREVEFGNDGDYSEDRFIKI 420

Query: 493 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEELS 552
           VNAHLANTIGNLLNRTLGLLKKNC+STLVVDST AAEG   KD VE+LV KAR +YE LS
Sbjct: 421 VNAHLANTIGNLLNRTLGLLKKNCESTLVVDSTVAAEGVPLKDTVEKLVEKARTNYENLS 480

Query: 553 LSSACEAVLEIGNAGNSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSPVT 612
           LSSACEAVLEIGNAGN+Y+D+RAPW LFKQGG S+  AAKDL++ILE MR+IAVALSPV 
Sbjct: 481 LSSACEAVLEIGNAGNTYMDQRAPWFLFKQGGVSAEEAAKDLVIILEVMRVIAVALSPVA 540

Query: 613 PCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPKPVFARIE 660
           PCLS RIY+QLG+++DQF + TW++TKWGGLK GQVM    PVFARIE
Sbjct: 541 PCLSLRIYSQLGYTEDQFNSITWSDTKWGGLKGGQVMEQASPVFARIE 581

BLAST of Sgr030463 vs. ExPASy Swiss-Prot
Match: Q55729 (Methionine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=metG PE=3 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 4.1e-159
Identity = 277/521 (53.17%), Postives = 357/521 (68.52%), Query Frame = 0

Query: 149 FVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQG 208
           F +TTPLYYVN  PH+GSAYTT+ AD +ARF+RL G  V+ ITGTDEHG+KI   A AQ 
Sbjct: 9   FSVTTPLYYVNDVPHLGSAYTTVVADTLARFKRLQGYDVLMITGTDEHGQKIQRTAEAQE 68

Query: 209 SDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVKEFYSKVLANGDIYRADYEG 268
            DP  HCD     +K LW+ L++ YD+F RTT+P+H AIVK+F+ +V   GDIY A  +G
Sbjct: 69  LDPQTHCDRTVVKFKELWRSLNILYDRFSRTTDPRHLAIVKDFFQRVWDKGDIYLAQQQG 128

Query: 269 LYCVNCEEYKDEKELLANNCCPIHLKPCAS-RKENNYFFALSKYQRSLEETLLQNPGFVQ 328
            YCV CEE+K++++LL +N CP+H    A  R E NYFF LS+YQ  LEE   Q P F+Q
Sbjct: 129 WYCVACEEFKEKRDLLEDNHCPLHPNRKAEWRDEENYFFRLSRYQHPLEELYAQRPEFIQ 188

Query: 329 PAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDALLGYISALSENGEQ 388
           P+ R NEV +++  GL+DFSISR +++WG P+PND   TIYVWFDALLGY++AL +  E+
Sbjct: 189 PSSRRNEVLNFVAQGLQDFSISRVNLDWGFPLPNDPNHTIYVWFDALLGYVTALLDEDEE 248

Query: 389 PKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMGK 448
           P L   +   WP +LHLIGKDILRFHAVYWPAMLMSA L +P  VFGHGFLTKDG KMGK
Sbjct: 249 PNLTNALVKWWPINLHLIGKDILRFHAVYWPAMLMSAELAIPAQVFGHGFLTKDGQKMGK 308

Query: 449 SLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRT 508
           SLGNT++P +L+ ++G DA RY+FL+E+EFG DGD+ E RF+N++NA LAN +GNLLNRT
Sbjct: 309 SLGNTVDPLDLINRYGEDAFRYYFLKEIEFGKDGDFNEQRFVNVLNADLANDLGNLLNRT 368

Query: 509 LGLLKKNCQST-LVVDSTAAAEGTAFKDKVEELVVKARNHYEELSLSSACEAVLEIGNAG 568
           LG++KK CQ     V +T  A     K     L  +  + YE LS + ACEA+  +  AG
Sbjct: 369 LGMVKKYCQGQGPQVMATDLAPDNPLKALGSHLGEEVSSAYERLSFTDACEAIFTLVRAG 428

Query: 569 NSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSPVTPCLSWRIYAQLGFSK 628
           N YID+ APW LFKQG  S       L  +LE++R+ A  LSP+ P LS +IY QLGF+ 
Sbjct: 429 NKYIDDMAPWKLFKQG--SQKEVEDVLYSVLESIRLSAYLLSPIVPRLSTKIYQQLGFTW 488

Query: 629 D-------QFEAATWN-ETKWGGLKAGQVMAPPKPVFARIE 660
           D         +A  +N    WG L A Q + P +P+F ++E
Sbjct: 489 DFDQWRSPLEQAEEFNRHQSWGQLGANQNLPPAQPIFTKLE 527

BLAST of Sgr030463 vs. ExPASy Swiss-Prot
Match: Q8Z068 (Methionine--tRNA ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=metG PE=3 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 3.8e-157
Identity = 279/523 (53.35%), Postives = 364/523 (69.60%), Query Frame = 0

Query: 145 AADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAA 204
           A   F +TTPLYYVN  PH+GSAYTT+AADA+ARFQ+LLG+ V+ ITGTDEHG+KI  +A
Sbjct: 7   AEKTFALTTPLYYVNDVPHIGSAYTTMAADAVARFQKLLGRDVLLITGTDEHGQKIQRSA 66

Query: 205 AAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVKEFYSKVLANGDIYRA 264
            + G  P E CD I  ++ SLW+ L++ YD+F RTT  +H+AIV EF+++V   GDIY+ 
Sbjct: 67  ESLGKAPQEFCDEIVPSFMSLWRLLNIQYDRFSRTTAVRHKAIVDEFFARVWEAGDIYQG 126

Query: 265 DYEGLYCVNCEEYKDEKELLANNCCPIHL-KPCASRKENNYFFALSKYQRSLEETLLQNP 324
             +G YCV+CEE+K+E+ELL  N CPIH+ K    R E NYFF LSKYQ  LEE    +P
Sbjct: 127 QQKGWYCVSCEEFKEERELLEGNRCPIHVNKEVEWRDEQNYFFRLSKYQTQLEEFYQSHP 186

Query: 325 GFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDALLGYISALSE 384
            F+QP  R NEV +++  GL+DFSISR +++WG PVPND K T+YVWFDALL Y++AL +
Sbjct: 187 DFIQPESRRNEVLNFVSQGLQDFSISRVNLDWGFPVPNDPKHTLYVWFDALLAYVTALLD 246

Query: 385 NGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGM 444
             ++P L+  +   WP +LHLIGKDILRFHAVYWPAML+SAGL LP  VFGHGFLTKDG 
Sbjct: 247 PEDEPTLENALGKWWPINLHLIGKDILRFHAVYWPAMLLSAGLPLPDRVFGHGFLTKDGQ 306

Query: 445 KMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTIGNL 504
           KMGKSLGNT++P  LVQ++GSDAVRY+FL+E+EFG DGD+ E RFI+++NA LAN +GNL
Sbjct: 307 KMGKSLGNTVDPVGLVQQYGSDAVRYYFLKEIEFGKDGDFNEVRFIHVLNADLANDLGNL 366

Query: 505 LNRTLGLLKKNC--QSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEELSLSSACEAVLE 564
           LNRTL ++KK C   +  + +    AE T  K    +L  K +  YE L+ + AC  VL 
Sbjct: 367 LNRTLNMVKKYCANYALSITNEDIPAENT-LKALGVDLGAKVKQAYEVLAFNQACGVVLS 426

Query: 565 IGNAGNSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSPVTPCLSWRIYAQ 624
           +  A N +ID++APWSL+KQ           L  +LE++R+ A  LSPV P +S  IY Q
Sbjct: 427 LVQASNKFIDDQAPWSLYKQERQQEVETV--LYTVLESVRLAAYLLSPVIPNISSNIYQQ 486

Query: 625 LGFS---KDQFEAATWN-ETKWGGLKAGQVMAPPKPVFARIEQ 661
           LGF     +Q +   +    +WG L   Q +  P+P+F RIEQ
Sbjct: 487 LGFGINFNEQTDITPFAIHAQWGLLSNKQQLGQPQPIFKRIEQ 526

BLAST of Sgr030463 vs. ExPASy Swiss-Prot
Match: P59081 (Methionine--tRNA ligase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=metG PE=3 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 1.4e-151
Identity = 265/518 (51.16%), Postives = 352/518 (67.95%), Query Frame = 0

Query: 149 FVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQG 208
           F +TTPLYYVNA PH+GSAYTTIAAD +ARF RL G +V FITGTDEHG+KI   A  +G
Sbjct: 5   FSLTTPLYYVNALPHIGSAYTTIAADVLARFYRLQGYQVRFITGTDEHGQKIERTAQQRG 64

Query: 209 SDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVKEFYSKVLANGDIYRADYEG 268
             P  HCD I+  +++LW+ L++ YD+F RTT+P+H AIV EF+ +V  NGDIY    +G
Sbjct: 65  LSPQAHCDEIAAGFQALWQQLNIHYDRFSRTTSPRHHAIVNEFFQRVWDNGDIYLGQQQG 124

Query: 269 LYCVNCEEYKDEKELLANNCCPIHLKPCAS-RKENNYFFALSKYQRSLEETLLQNPGFVQ 328
            YCV CEE+K+E+ELL    CPIH+      R E NYFF LSKYQ++L +   ++P FVQ
Sbjct: 125 WYCVECEEFKEERELLEGRRCPIHVNRTVEWRDERNYFFRLSKYQQALLDHYAEHPDFVQ 184

Query: 329 PAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDALLGYISALSENGEQ 388
           P  R NEV S+I  GL+DFSISR ++ WG PVP D +QT+YVWFDALLGY++AL E  ++
Sbjct: 185 PPSRRNEVLSFIERGLQDFSISRVNLAWGFPVPTDPEQTLYVWFDALLGYVTALLEPEDE 244

Query: 389 PKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMGK 448
           P L   + + WP +LH+IGKDILRFH + WPAMLMSAGL LP+ +F HGFLTKDG KMGK
Sbjct: 245 PTLANALKTWWPINLHIIGKDILRFHGISWPAMLMSAGLPLPEQIFVHGFLTKDGQKMGK 304

Query: 449 SLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRT 508
           SLGNTL+P  LV ++G+DAVRY+F++EVEFG DGD++E RF+ I+NA LAN +GNLLNRT
Sbjct: 305 SLGNTLDPFALVAQYGADAVRYYFMKEVEFGRDGDFSETRFVTILNADLANDLGNLLNRT 364

Query: 509 LGLLKKNCQSTLV-VDSTAAAEGTAFKDKVEELVVKARNHYEELSLSSACEAVLEIGNAG 568
           L +  K     +  V   A       +   E L       Y +L+    C+  L +  AG
Sbjct: 365 LKMAWKYTDGKVPNVQGAAIPREHPLRQLAEHLSQTYGQGYRQLAFHEVCQQALTLARAG 424

Query: 569 NSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSPVTPCLSWRIYAQLGFSK 628
           N ++DE APW  ++ G   +AA A+ L  +LE++R++A  L+P+ P LS  IY QLG+S 
Sbjct: 425 NKFLDEEAPWKRYRAG--ETAAVAEILYCVLESVRLVAYVLAPIIPQLSEAIYGQLGYS- 484

Query: 629 DQFEAATW------NETKWGGLKAGQVMAPPKPVFARI 659
            +F  +         + +WG L A Q +A P+P+F ++
Sbjct: 485 IRFNGSIAPDLLGDRQAQWGVLPASQPLANPEPIFQKL 519

BLAST of Sgr030463 vs. ExPASy Swiss-Prot
Match: O67298 (Methionine--tRNA ligase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=metG PE=1 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 9.1e-119
Identity = 217/466 (46.57%), Postives = 290/466 (62.23%), Query Frame = 0

Query: 149 FVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQG 208
           F +TTP+YYVN  PH+G AYTTIAAD IAR+ RL    V F+TGTDEHG KI   A   G
Sbjct: 7   FYVTTPIYYVNDVPHLGHAYTTIAADTIARYYRLRDYDVFFLTGTDEHGLKIQKKAEELG 66

Query: 209 SDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVKEFYSKVLANGDIYRADYEG 268
             P E  D  +E +K LW+ L + Y KFIRTT+P H   V++ + +    GDIY  +YEG
Sbjct: 67  ISPKELVDRNAERFKKLWEFLKIEYTKFIRTTDPYHVKFVQKVFEECYKRGDIYLGEYEG 126

Query: 269 LYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFFALSKYQRSLEETLLQNPGFVQP 328
            YCV CEE+K E EL  ++ CPIH K C   KE +YFF LSKYQ  L E   +NP F+QP
Sbjct: 127 WYCVGCEEFKSEAELAEDHTCPIHQKKCEYIKEPSYFFRLSKYQDKLLELYEKNPEFIQP 186

Query: 329 AFRLNEVQSWIRSGLKDFSIS--RASVEWGIPVPNDQKQTIYVWFDALLGYISALSENGE 388
            +R NE+ S+++ GLKD S++  R+ V+WGIPVP D + TIYVWFDAL  YISAL +   
Sbjct: 187 DYRRNEIISFVKQGLKDLSVTRPRSRVKWGIPVPFDPEHTIYVWFDALFNYISALEDK-- 246

Query: 389 QPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMG 448
                  V   WPA LHL+GKDILRFH VYWPA LMS G +LPK VF HG+ T +G KM 
Sbjct: 247 -------VEIYWPADLHLVGKDILRFHTVYWPAFLMSLGYELPKKVFAHGWWTVEGKKMS 306

Query: 449 KSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTIGNLLNR 508
           K+LGN ++P E+VQ++G D VRYF LREV FG DGD+++   +N +N  LAN IGNL +R
Sbjct: 307 KTLGNVVDPYEVVQEYGLDEVRYFLLREVPFGQDGDFSKKAILNRINGELANEIGNLYSR 366

Query: 509 TLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEELSLSSACEAVLEIGNAG 568
            + +  K      +    + A    +    +E +    N+ E+++   A E +L+  +  
Sbjct: 367 VVNMAHK-----FLGGEVSGARDEEYAKIAQESIKNYENYMEKVNFYKAIEEILKFTSYL 426

Query: 569 NSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSPVTP 613
           N Y+DE+ PW+L K+         K L  +++ + ++   L P+TP
Sbjct: 427 NKYVDEKQPWALNKE--RKKEELQKVLYALVDGLFVLTHLLYPITP 456

BLAST of Sgr030463 vs. ExPASy TrEMBL
Match: A0A0A0KQV7 (Methionine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_5G512910 PE=3 SV=1)

HSP 1 Score: 1072.0 bits (2771), Expect = 9.9e-310
Identity = 536/592 (90.54%), Postives = 555/592 (93.75%), Query Frame = 0

Query: 73  MGARINTSIQSSLWLLNPLQ---NLKATHLRTRLHFSRTLFSSPSSSSSSSSSPSSKRAI 132
           M ARINTSIQ+SLWLLNP     NLK TH RTRLHF + L S PS S       SSKRAI
Sbjct: 1   MAARINTSIQTSLWLLNPFHSSLNLKPTHFRTRLHFCQNLLSFPSHS-------SSKRAI 60

Query: 133 FCTSASNSNSHEREPAADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 192
           FCTS SN NSH+   A DPFVITTPLYYVNAAPHMGSAYTTIAADA+ARFQRLLGKKVIF
Sbjct: 61  FCTSTSNFNSHDSGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIF 120

Query: 193 ITGTDEHGEKIATAAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVK 252
           ITGTDEHGEKIATAAA QGS PSEHCD ISEAYK+LWKDLDVAYDKFIRTT+PKHEAIVK
Sbjct: 121 ITGTDEHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVK 180

Query: 253 EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFFALS 312
           EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKE+NYFFALS
Sbjct: 181 EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240

Query: 313 KYQRSLEETLLQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYV 372
           KYQR LEETL QNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND+KQTIYV
Sbjct: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYV 300

Query: 373 WFDALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 432
           WFDALLGYISALSENGEQP LQ +VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP
Sbjct: 301 WFDALLGYISALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360

Query: 433 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFI 492
           KMVFGHGFLTKDGMKMGKSLGNTLEPNELV+KFGSDAVRYFFLREVEFGSDGDYAEDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFI 420

Query: 493 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEE 552
           NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAA+G AFKDKVEELVVKARN+YEE
Sbjct: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEE 480

Query: 553 LSLSSACEAVLEIGNAGNSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSP 612
           L+LSSACEAVLEIGNAGNSY+DERAPWSLFKQGGASS AAAKDL+LILEAMRIIA+ALSP
Sbjct: 481 LALSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSP 540

Query: 613 VTPCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPKPVFARIEQA 662
           VTP LSWRIYAQLG+SKDQF+AATWNETKWGGLKAGQVMAPP PVFARIEQA
Sbjct: 541 VTPRLSWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQA 585

BLAST of Sgr030463 vs. ExPASy TrEMBL
Match: A0A6J1BXG8 (Methionine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111006379 PE=3 SV=1)

HSP 1 Score: 1068.5 bits (2762), Expect = 1.1e-308
Identity = 542/596 (90.94%), Postives = 558/596 (93.62%), Query Frame = 0

Query: 73  MGARINTSIQSSLWLLNPLQ-----NLKAT-HLRTRLHFSRTLFSSPSSSSSSSSSPSSK 132
           M ARIN+SIQSSLWLLNPLQ     NL    +LRTRL+F R L  SPS S+S     SSK
Sbjct: 1   MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSAS-----SSK 60

Query: 133 RAIFCTSASNSNSHEREPAADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKK 192
           RAIFCT ASN NSHE   A DPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKK
Sbjct: 61  RAIFCTYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKK 120

Query: 193 VIFITGTDEHGEKIATAAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEA 252
           VIFITGTDEHGEKIATAAAAQGS PSEHCDAISEAYK+LWKDLDVAYDKFIRTT+PKHEA
Sbjct: 121 VIFITGTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEA 180

Query: 253 IVKEFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFF 312
           IVKEFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKE+NYFF
Sbjct: 181 IVKEFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFF 240

Query: 313 ALSKYQRSLEETLLQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQT 372
           ALSKYQ  LEETL QNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQT
Sbjct: 241 ALSKYQGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQT 300

Query: 373 IYVWFDALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGL 432
           IYVWFDALLGYISALSENG+Q  LQR VASGWPASLHLIGKDILRFHAVYWPAMLMSAGL
Sbjct: 301 IYVWFDALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGL 360

Query: 433 KLPKMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAED 492
           KLPKMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFL+EVEFGSDGDYAED
Sbjct: 361 KLPKMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAED 420

Query: 493 RFINIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNH 552
           RFINIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEG +FKDKVEELV+KARNH
Sbjct: 421 RFINIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNH 480

Query: 553 YEELSLSSACEAVLEIGNAGNSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVA 612
           YE LSLSSACEAVLEIGNAGNSY+DE+APWSLFKQGGASS AAAKDL+LILEAMRIIAVA
Sbjct: 481 YEGLSLSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVA 540

Query: 613 LSPVTPCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPKPVFARIEQAS 663
           LSPVTPCL WRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPP PVFARIEQ++
Sbjct: 541 LSPVTPCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQST 591

BLAST of Sgr030463 vs. ExPASy TrEMBL
Match: A0A6J1H8X3 (Methionine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111461165 PE=3 SV=1)

HSP 1 Score: 1062.4 bits (2746), Expect = 7.9e-307
Identity = 535/592 (90.37%), Postives = 554/592 (93.58%), Query Frame = 0

Query: 73  MGARINTSIQSSLWLLNPLQ---NLKATHLRTRLHFSRTLFSSPSSSSSSSSSPSSKRAI 132
           M ARINTSIQ+SLWLLNPLQ   NLKATHLRTR +F + L S+PSS SSS    SSKRAI
Sbjct: 1   MAARINTSIQNSLWLLNPLQSSRNLKATHLRTRFNFYQNLLSAPSSCSSS----SSKRAI 60

Query: 133 FCTSASNSNSHEREPAADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 192
           FC+SASN NSH+   A DPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF
Sbjct: 61  FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120

Query: 193 ITGTDEHGEKIATAAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVK 252
           ITGTDEHGEKIATAAAAQGS PSEHCD ISEAYK+LWKDLD+AYDKFIRTT+PKHE IVK
Sbjct: 121 ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK 180

Query: 253 EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFFALS 312
           EFYS VLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKE+NYFFALS
Sbjct: 181 EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240

Query: 313 KYQRSLEETLLQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYV 372
           KYQR LEETL QNPGFVQPAFRLNEVQSWI+SGLKDFSISRASVEWGIPVPNDQKQTIYV
Sbjct: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV 300

Query: 373 WFDALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 432
           WFDALLGYISALSEN EQP LQ  VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP
Sbjct: 301 WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360

Query: 433 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFI 492
           KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSD VRYFFLREVEFGSDGDYAEDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI 420

Query: 493 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEE 552
           NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDS+AAA G AFK+KVEELV KARNHYEE
Sbjct: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE 480

Query: 553 LSLSSACEAVLEIGNAGNSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSP 612
           LSLSSACEAVLEIGNAGNSY+DERAPWSLFKQGGASS AAAKDL+LILEAMRIIA+ALSP
Sbjct: 481 LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP 540

Query: 613 VTPCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPKPVFARIEQA 662
           VTP LSWRIY QLG+ +DQF AATWNETKWGGLKAGQVMAPP PVFARIEQA
Sbjct: 541 VTPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQA 588

BLAST of Sgr030463 vs. ExPASy TrEMBL
Match: A0A5D3C1K0 (Methionine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G003330 PE=3 SV=1)

HSP 1 Score: 1059.7 bits (2739), Expect = 5.1e-306
Identity = 532/592 (89.86%), Postives = 552/592 (93.24%), Query Frame = 0

Query: 73  MGARINTSIQSSLWLLNPLQ---NLKATHLRTRLHFSRTLFSSPSSSSSSSSSPSSKRAI 132
           M ARINTSIQ+SL LLNP     NLK TH RTRLHF + L S  S S       SSKRAI
Sbjct: 1   MAARINTSIQTSLSLLNPFHNSLNLKPTHFRTRLHFYQNLLSFASYS-------SSKRAI 60

Query: 133 FCTSASNSNSHEREPAADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 192
           FCT+ SN NSH+   A DPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVI 
Sbjct: 61  FCTTTSNFNSHDTGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIL 120

Query: 193 ITGTDEHGEKIATAAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVK 252
           ITGTDEHGEKIATAAA QGS PSEHCD ISEAYK+LWKDLDVAYDKFIRTT+PKHEAIVK
Sbjct: 121 ITGTDEHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVK 180

Query: 253 EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFFALS 312
           EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKE+NYFFALS
Sbjct: 181 EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240

Query: 313 KYQRSLEETLLQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYV 372
           KYQR LEETL QNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND+KQTIYV
Sbjct: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDKKQTIYV 300

Query: 373 WFDALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 432
           WFDALLGYISALSENGEQP L ++VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP
Sbjct: 301 WFDALLGYISALSENGEQPNLHKVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360

Query: 433 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFI 492
           KMVFGHGFLTKDGMKMGKSLGNTLEPNELV+KFGSDAVRYFFLREVEFGSDGDYAEDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFI 420

Query: 493 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEE 552
           NIVNAHLANTIGNLLNRTLGLLKKNCQSTL+VDSTAAA+G AFKDKVEELVVKARNHYEE
Sbjct: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLLVDSTAAADGNAFKDKVEELVVKARNHYEE 480

Query: 553 LSLSSACEAVLEIGNAGNSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSP 612
           L+LSSACEAVLEIGNAGNSY+DERAPWSLFKQGGASS AAAKDL+LILEAMRIIA+ALSP
Sbjct: 481 LALSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSP 540

Query: 613 VTPCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPKPVFARIEQA 662
           VTP LSWRIYAQLG+SKDQF+AATWNETKWGGLKAGQVMAPP PVFARIEQA
Sbjct: 541 VTPRLSWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQA 585

BLAST of Sgr030463 vs. ExPASy TrEMBL
Match: A0A1S3CJS7 (Methionine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103501636 PE=3 SV=1)

HSP 1 Score: 1058.9 bits (2737), Expect = 8.7e-306
Identity = 532/592 (89.86%), Postives = 551/592 (93.07%), Query Frame = 0

Query: 73  MGARINTSIQSSLWLLNPLQ---NLKATHLRTRLHFSRTLFSSPSSSSSSSSSPSSKRAI 132
           M ARINTSIQ+SL LLNP     NLK TH RTRLHF + L S  S S       SSKRAI
Sbjct: 1   MAARINTSIQTSLSLLNPFHNSLNLKPTHFRTRLHFYQNLLSFASYS-------SSKRAI 60

Query: 133 FCTSASNSNSHEREPAADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 192
           FCT+ SN NSH+   A DPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVI 
Sbjct: 61  FCTTTSNFNSHDTGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIL 120

Query: 193 ITGTDEHGEKIATAAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVK 252
           ITGTDEHGEKIATAAA QGS PSEHCD ISEAYK+LWKDLDVAYDKFIRTT+PKHEAIVK
Sbjct: 121 ITGTDEHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVK 180

Query: 253 EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFFALS 312
           EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKE+NYFFALS
Sbjct: 181 EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240

Query: 313 KYQRSLEETLLQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYV 372
           KYQR LEETL QNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND KQTIYV
Sbjct: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDXKQTIYV 300

Query: 373 WFDALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 432
           WFDALLGYISALSENGEQP L ++VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP
Sbjct: 301 WFDALLGYISALSENGEQPNLHKVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360

Query: 433 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFI 492
           KMVFGHGFLTKDGMKMGKSLGNTLEPNELV+KFGSDAVRYFFLREVEFGSDGDYAEDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFI 420

Query: 493 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEE 552
           NIVNAHLANTIGNLLNRTLGLLKKNCQSTL+VDSTAAA+G AFKDKVEELVVKARNHYEE
Sbjct: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLLVDSTAAADGNAFKDKVEELVVKARNHYEE 480

Query: 553 LSLSSACEAVLEIGNAGNSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSP 612
           L+LSSACEAVLEIGNAGNSY+DERAPWSLFKQGGASS AAAKDL+LILEAMRIIA+ALSP
Sbjct: 481 LALSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSP 540

Query: 613 VTPCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPKPVFARIEQA 662
           VTP LSWRIYAQLG+SKDQF+AATWNETKWGGLKAGQVMAPP PVFARIEQA
Sbjct: 541 VTPRLSWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQA 585

BLAST of Sgr030463 vs. TAIR 10
Match: AT3G55400.1 (methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) )

HSP 1 Score: 890.2 bits (2299), Expect = 1.0e-258
Identity = 441/588 (75.00%), Postives = 500/588 (85.03%), Query Frame = 0

Query: 73  MGARINTSIQSSLWLLNPLQNLKATHLRTR-LHFSRTLFSSPSSSSSSSSSPSSKRAIFC 132
           M ARINTS+ ++L  L P      T L T+   F R  F         S   S KRA++C
Sbjct: 1   MAARINTSLHNALSFLKPFN----TPLNTKPFSFRRNSFRFSKKLPYYSQFSSGKRALYC 60

Query: 133 TSASNSNSHEREPAADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 192
           TS+S  ++ +     + FV+TTPLYYVNA PHMGSAYTTIAAD+IARFQRLLGKKVIFIT
Sbjct: 61  TSSSQESTVDE---GETFVLTTPLYYVNAPPHMGSAYTTIAADSIARFQRLLGKKVIFIT 120

Query: 193 GTDEHGEKIATAAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVKEF 252
           GTDEHGEKIAT+AAA G +P EHCD IS++Y++LWKDLD+AYDKFIRTT+PKHEAIVKEF
Sbjct: 121 GTDEHGEKIATSAAANGRNPPEHCDLISQSYRTLWKDLDIAYDKFIRTTDPKHEAIVKEF 180

Query: 253 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFFALSKY 312
           Y++V ANGDIYRADYEGLYCVNCEEYKDEKELL NNCCP+H  PC +RKE+NYFFALSKY
Sbjct: 181 YARVFANGDIYRADYEGLYCVNCEEYKDEKELLENNCCPVHQMPCVARKEDNYFFALSKY 240

Query: 313 QRSLEETLLQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 372
           Q+ LE+ L QNP FVQP++RLNEVQSWI+SGL+DFSISRA V+WGIPVP+D KQTIYVWF
Sbjct: 241 QKPLEDILAQNPRFVQPSYRLNEVQSWIKSGLRDFSISRALVDWGIPVPDDDKQTIYVWF 300

Query: 373 DALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 432
           DALLGYISAL+E+ +Q  L+  V+ GWPASLHLIGKDILRFHAVYWPAMLMSAGL+LPKM
Sbjct: 301 DALLGYISALTEDNKQQNLETAVSFGWPASLHLIGKDILRFHAVYWPAMLMSAGLELPKM 360

Query: 433 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINI 492
           VFGHGFLTKDGMKMGKSLGNTLEP ELVQKFG DAVRYFFLREVEFG+DGDY+EDRFI I
Sbjct: 361 VFGHGFLTKDGMKMGKSLGNTLEPFELVQKFGPDAVRYFFLREVEFGNDGDYSEDRFIKI 420

Query: 493 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEELS 552
           VNAHLANTIGNLLNRTLGLLKKNC+STLVVDST AAEG   KD VE+LV KAR +YE LS
Sbjct: 421 VNAHLANTIGNLLNRTLGLLKKNCESTLVVDSTVAAEGVPLKDTVEKLVEKARTNYENLS 480

Query: 553 LSSACEAVLEIGNAGNSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSPVT 612
           LSSACEAVLEIGNAGN+Y+D+RAPW LFKQGG S+  AAKDL++ILE MR+IAVALSPV 
Sbjct: 481 LSSACEAVLEIGNAGNTYMDQRAPWFLFKQGGVSAEEAAKDLVIILEVMRVIAVALSPVA 540

Query: 613 PCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPKPVFARIE 660
           PCLS RIY+QLG+++DQF + TW++TKWGGLK GQVM    PVFARIE
Sbjct: 541 PCLSLRIYSQLGYTEDQFNSITWSDTKWGGLKGGQVMEQASPVFARIE 581

BLAST of Sgr030463 vs. TAIR 10
Match: AT3G55400.2 (methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) )

HSP 1 Score: 788.9 bits (2036), Expect = 3.2e-228
Identity = 394/522 (75.48%), Postives = 443/522 (84.87%), Query Frame = 0

Query: 73  MGARINTSIQSSLWLLNPLQNLKATHLRTR-LHFSRTLFSSPSSSSSSSSSPSSKRAIFC 132
           M ARINTS+ ++L  L P      T L T+   F R  F         S   S KRA++C
Sbjct: 1   MAARINTSLHNALSFLKPFN----TPLNTKPFSFRRNSFRFSKKLPYYSQFSSGKRALYC 60

Query: 133 TSASNSNSHEREPAADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 192
           TS+S  ++ +     + FV+TTPLYYVNA PHMGSAYTTIAAD+IARFQRLLGKKVIFIT
Sbjct: 61  TSSSQESTVDE---GETFVLTTPLYYVNAPPHMGSAYTTIAADSIARFQRLLGKKVIFIT 120

Query: 193 GTDEHGEKIATAAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVKEF 252
           GTDEHGEKIAT+AAA G +P EHCD IS++Y++LWKDLD+AYDKFIRTT+PKHEAIVKEF
Sbjct: 121 GTDEHGEKIATSAAANGRNPPEHCDLISQSYRTLWKDLDIAYDKFIRTTDPKHEAIVKEF 180

Query: 253 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFFALSKY 312
           Y++V ANGDIYRADYEGLYCVNCEEYKDEKELL NNCCP+H  PC +RKE+NYFFALSKY
Sbjct: 181 YARVFANGDIYRADYEGLYCVNCEEYKDEKELLENNCCPVHQMPCVARKEDNYFFALSKY 240

Query: 313 QRSLEETLLQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 372
           Q+ LE+ L QNP FVQP++RLNEVQSWI+SGL+DFSISRA V+WGIPVP+D KQTIYVWF
Sbjct: 241 QKPLEDILAQNPRFVQPSYRLNEVQSWIKSGLRDFSISRALVDWGIPVPDDDKQTIYVWF 300

Query: 373 DALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 432
           DALLGYISAL+E+ +Q  L+  V+ GWPASLHLIGKDILRFHAVYWPAMLMSAGL+LPKM
Sbjct: 301 DALLGYISALTEDNKQQNLETAVSFGWPASLHLIGKDILRFHAVYWPAMLMSAGLELPKM 360

Query: 433 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINI 492
           VFGHGFLTKDGMKMGKSLGNTLEP ELVQKFG DAVRYFFLREVEFG+DGDY+EDRFI I
Sbjct: 361 VFGHGFLTKDGMKMGKSLGNTLEPFELVQKFGPDAVRYFFLREVEFGNDGDYSEDRFIKI 420

Query: 493 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEELS 552
           VNAHLANTIGNLLNRTLGLLKKNC+STLVVDST AAEG   KD VE+LV KAR +YE LS
Sbjct: 421 VNAHLANTIGNLLNRTLGLLKKNCESTLVVDSTVAAEGVPLKDTVEKLVEKARTNYENLS 480

Query: 553 LSSACEAVLEIGNAGNSYIDERAPWSLFKQGGASSAAAAKDL 594
           LSSACEAVLEIGNAGN+Y+D+RAPW LFKQGG S+  AAK L
Sbjct: 481 LSSACEAVLEIGNAGNTYMDQRAPWFLFKQGGVSAEEAAKVL 515

BLAST of Sgr030463 vs. TAIR 10
Match: AT4G13780.1 (methionine--tRNA ligase, putative / methionyl-tRNA synthetase, putative / MetRS, putative )

HSP 1 Score: 161.4 bits (407), Expect = 2.5e-39
Identity = 144/583 (24.70%), Postives = 244/583 (41.85%), Query Frame = 0

Query: 144 PAADPFVITTPLYYVNAAPHMGSAY-TTIAADAIARFQRLLGKKVIFITGTDEHGEKIAT 203
           P     +IT+ L YVN  PH+G+     ++AD  AR+ RL G   I+I GTDE+G    T
Sbjct: 14  PGKRNILITSALPYVNNVPHLGNIIGCVLSADVYARYCRLRGYNAIYICGTDEYGTATET 73

Query: 204 AAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVKEFYSKVLANGDIY 263
            A  +   P E CD     +K ++    +++DKF RT+ P+   + +  ++K+  N  + 
Sbjct: 74  KALEENCTPKEICDKYHAIHKEVYDWFGISFDKFGRTSTPEQTEVCQAIFNKLWDNKWLS 133

Query: 264 RADYEGLYCVNCEEYKDEKELLANNCCPIH----------------------------LK 323
               + LYC  C+++  ++  L    CP                               K
Sbjct: 134 ENTMQQLYCDTCKKFLADR--LVEGSCPFEGCNYDSARGDQCEKCGKLLNPTELKDPKCK 193

Query: 324 PCAS----RKENNYFFALSKYQRSLEETLLQNPGFVQPAFRLNEVQ---SWIRSGLKDFS 383
            C +    R  ++ F  L   +  LE  + +    V  ++  N +Q   +W+R GL+   
Sbjct: 194 VCQNTPRIRDTDHLFIELPLLKDRLEAYIKKTS--VTGSWSQNAIQTTNAWLRDGLRQRC 253

Query: 384 ISRASVEWGIPVPND--QKQTIYVWFDALLGYISALSENGEQPKLQRIVASGW------P 443
           I+R  ++WG+PVP++  + +  YVWFDA +GY+S  S             S W      P
Sbjct: 254 ITR-DLKWGVPVPHEKYKDKVFYVWFDAPIGYVSITS----------CYTSEWEKWWKNP 313

Query: 444 ASLHL---IGKDILRFHAVYWPAMLMSA--GLKLPKMVFGHGFLTKDGMKMGKSLGNTLE 503
            ++ L   +GKD + FH V +P+  +       L K +    +L  +  K  KS G  + 
Sbjct: 314 ENVELYQFMGKDNVPFHTVMFPSTQLGTEENWTLMKTISVTEYLNYEDGKFSKSKGVGVF 373

Query: 504 PNELVQ-KFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRTLGLLKK 563
            N++       +  RY+ L      SD  ++       +N  L + +GN +NR L  + K
Sbjct: 374 GNDVKDTNIPVEVWRYYLLTNRPEVSDTSFSWTDLQAKLNGELLSNLGNFVNRVLSFIAK 433

Query: 564 --NCQSTLVVDSTAAAEG----TAFKDKVEELVVKARNHYEELSLSSACEAVLEIGNAGN 623
             N     V+     AE      +  +KVE+ V +     E++ L    +  + I + GN
Sbjct: 434 PDNAGYGSVIPDAHDAESHSLTKSLAEKVEKFVAEYVEAMEKVKLKQGLKTAMLISSEGN 493

Query: 624 SYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSPVTPCLSWRIYAQLG---- 666
            Y+     W L+K+     A   +    +   + ++A  L P  P  S  ++ QL     
Sbjct: 494 YYLQASQFWKLYKEDKPLCAVVIRTAAGL---VHLLAQLLEPFMPSFSCEVFKQLNLPPQ 553

BLAST of Sgr030463 vs. TAIR 10
Match: AT5G16715.1 (ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases )

HSP 1 Score: 47.8 bits (112), Expect = 4.1e-05
Identity = 62/257 (24.12%), Postives = 109/257 (42.41%), Query Frame = 0

Query: 398 WPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMV-FGHGFL-----TKDGMKMGKSLGN 457
           +P ++   G DIL F    W A ++  G++    V F H +L        G KM KSLGN
Sbjct: 550 YPTNMLETGHDILFF----WVARMVMMGIEFTGTVPFSHVYLHGLIRDSQGRKMSKSLGN 609

Query: 458 TLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTI---GNLLNRTL 517
            ++P + ++ FG+DA+R F +     G D + + +R     N    N +   G  +  +L
Sbjct: 610 VIDPLDTIKDFGTDALR-FTIALGTAGQDLNLSTERL--TANKAFTNKLWNAGKFVLHSL 669

Query: 518 GLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEELSLSSACEAVLEIGNAGNS 577
             L        ++D     E T     + E    ++ H    S++++ E  L  G+ G  
Sbjct: 670 PSLSDTSAWENLLDLKLDKEETLLSLPLPECWAVSKLHILIDSVTASYEK-LFFGDVGRE 729

Query: 578 YID----ERAPW-------SLFKQGGASSAAAAKDLILILEAMRIIAVALSPVTPCLSWR 635
             D    + A W        L+  GG S + A++ ++L +     I   L P  P ++  
Sbjct: 730 TYDFFWSDFADWYIEASKSRLYGSGGNSVSLASQAVLLYV--FENILKLLHPFMPFVTED 789

BLAST of Sgr030463 vs. TAIR 10
Match: AT5G49030.1 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 47.0 bits (110), Expect = 6.9e-05
Identity = 76/313 (24.28%), Postives = 126/313 (40.26%), Query Frame = 0

Query: 366 TIYVWFDALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSA- 425
           T+ VWFD+   +   L   G++  L       +PA ++L G D    H  ++ + L+++ 
Sbjct: 652 TMDVWFDSGSSWAGVL---GKREGL------SFPADVYLEGTD---QHRGWFQSSLLTSI 711

Query: 426 ---GLKLPKMVFGHGF-LTKDGMKMGKSLGNTLEPNELVQ---------KFGSDAVRYFF 485
              G      V  HGF L + GMKM KSLGN ++P  +++          +G+D +R + 
Sbjct: 712 ATQGKAPYSAVITHGFVLDEKGMKMSKSLGNVVDPRLVIEGGKNSKDAPAYGADVMRLWV 771

Query: 486 LREVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTA 545
                  S  DY  D  I        + I   L  TL  L  N      VD+    +   
Sbjct: 772 -------SSVDYTGDVLIGPQILRQMSDIYRKLRGTLRYLLGNLHDWR-VDNAVPYQDLP 831

Query: 546 FKD-----KVEELVVKARNHYEELSLSSACEAV--LEIGNAGNSYIDERAPWSLFKQGGA 605
             D     ++E +V   +  YE        + +    I +  N Y D  A   L+  G +
Sbjct: 832 IIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVDLSNFYFD-IAKDRLYTGGTS 891

Query: 606 SSAAAAKDLILILEAMRIIAVALSPVTPCLSWRIYAQLGF---SKDQFEAATWNETKWGG 655
           S    +   +L    + I+ V ++P+ P L+  ++  L F   ++D   A    E KW  
Sbjct: 892 SFTRRSCQTVLSTHLLSILRV-IAPIVPHLAEDVWQNLPFEYRNEDGSAAEFVFELKWPT 942

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004142742.12.0e-30990.54methionine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN51284... [more]
XP_022133974.12.2e-30890.94methionine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia][more]
KAG7033041.11.1e-30790.54Methionine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyros... [more]
XP_038891037.13.3e-30789.70methionine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida][more]
XP_022960433.11.6e-30690.37methionine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9M2T91.4e-25775.00Methionine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=... [more]
Q557294.1e-15953.17Methionine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=11117... [more]
Q8Z0683.8e-15753.35Methionine--tRNA ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) ... [more]
P590811.4e-15151.16Methionine--tRNA ligase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221... [more]
O672989.1e-11946.57Methionine--tRNA ligase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=metG PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A0A0KQV79.9e-31090.54Methionine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_5G512910 PE=3 SV=1[more]
A0A6J1BXG81.1e-30890.94Methionine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111006379 PE=3 SV=1[more]
A0A6J1H8X37.9e-30790.37Methionine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111461165 PE=3 SV=1[more]
A0A5D3C1K05.1e-30689.86Methionine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3CJS78.7e-30689.86Methionine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103501636 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G55400.11.0e-25875.00methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) [more]
AT3G55400.23.2e-22875.48methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) [more]
AT4G13780.12.5e-3924.70methionine--tRNA ligase, putative / methionyl-tRNA synthetase, putative / MetRS,... [more]
AT5G16715.14.1e-0524.12ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP bi... [more]
AT5G49030.16.9e-0524.28tRNA synthetase class I (I, L, M and V) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR033911Methioninyl-tRNA synthetase core domainPRINTSPR01041TRNASYNTHMETcoord: 183..197
score: 54.81
coord: 367..378
score: 72.53
coord: 403..418
score: 65.28
coord: 151..164
score: 56.61
coord: 231..242
score: 49.07
coord: 495..506
score: 51.85
IPR033911Methioninyl-tRNA synthetase core domainCDDcd00814MetRS_corecoord: 148..483
e-value: 7.62678E-168
score: 480.876
IPR002300Aminoacyl-tRNA synthetase, class IaPFAMPF00133tRNA-synt_1coord: 141..266
e-value: 1.6E-8
score: 33.3
IPR015413Methionyl/Leucyl tRNA synthetasePFAMPF09334tRNA-synt_1gcoord: 283..508
e-value: 1.9E-61
score: 208.0
IPR013155Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-bindingPFAMPF08264Anticodon_1coord: 535..638
e-value: 1.3E-5
score: 25.2
NoneNo IPR availableGENE3D2.170.220.10coord: 260..369
e-value: 6.0E-140
score: 468.0
NoneNo IPR availableGENE3D1.10.730.10coord: 484..663
e-value: 5.4E-43
score: 148.6
NoneNo IPR availablePANTHERPTHR43326METHIONYL-TRNA SYNTHETASEcoord: 73..660
NoneNo IPR availablePANTHERPTHR43326:SF6BNAA09G34980D PROTEINcoord: 73..660
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 147..493
IPR014758Methionyl-tRNA synthetaseTIGRFAMTIGR00398TIGR00398coord: 150..658
e-value: 2.1E-156
score: 519.7
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 149..480
e-value: 6.0E-140
score: 468.0
IPR023457Methionine-tRNA synthetase, type 2HAMAPMF_01228Met_tRNA_synth_type2coord: 140..654
score: 42.752636
IPR041872Methionyl-tRNA synthetase, anticodon-binding domainCDDcd07957Anticodon_Ia_Metcoord: 492..622
e-value: 4.36246E-34
score: 124.524
IPR009080Aminoacyl-tRNA synthetase, class Ia, anticodon-bindingSUPERFAMILY47323Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetasescoord: 496..658

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr030463.1Sgr030463.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006431 methionyl-tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005524 ATP binding
molecular_function GO:0004825 methionine-tRNA ligase activity
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding