Homology
BLAST of Sgr030463 vs. NCBI nr
Match:
XP_004142742.1 (methionine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN51284.1 hypothetical protein Csa_008274 [Cucumis sativus])
HSP 1 Score: 1072.0 bits (2771), Expect = 2.0e-309
Identity = 536/592 (90.54%), Postives = 555/592 (93.75%), Query Frame = 0
Query: 73 MGARINTSIQSSLWLLNPLQ---NLKATHLRTRLHFSRTLFSSPSSSSSSSSSPSSKRAI 132
M ARINTSIQ+SLWLLNP NLK TH RTRLHF + L S PS S SSKRAI
Sbjct: 1 MAARINTSIQTSLWLLNPFHSSLNLKPTHFRTRLHFCQNLLSFPSHS-------SSKRAI 60
Query: 133 FCTSASNSNSHEREPAADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 192
FCTS SN NSH+ A DPFVITTPLYYVNAAPHMGSAYTTIAADA+ARFQRLLGKKVIF
Sbjct: 61 FCTSTSNFNSHDSGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIF 120
Query: 193 ITGTDEHGEKIATAAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVK 252
ITGTDEHGEKIATAAA QGS PSEHCD ISEAYK+LWKDLDVAYDKFIRTT+PKHEAIVK
Sbjct: 121 ITGTDEHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVK 180
Query: 253 EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFFALS 312
EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKE+NYFFALS
Sbjct: 181 EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240
Query: 313 KYQRSLEETLLQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYV 372
KYQR LEETL QNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND+KQTIYV
Sbjct: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYV 300
Query: 373 WFDALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 432
WFDALLGYISALSENGEQP LQ +VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP
Sbjct: 301 WFDALLGYISALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360
Query: 433 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFI 492
KMVFGHGFLTKDGMKMGKSLGNTLEPNELV+KFGSDAVRYFFLREVEFGSDGDYAEDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFI 420
Query: 493 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEE 552
NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAA+G AFKDKVEELVVKARN+YEE
Sbjct: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEE 480
Query: 553 LSLSSACEAVLEIGNAGNSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSP 612
L+LSSACEAVLEIGNAGNSY+DERAPWSLFKQGGASS AAAKDL+LILEAMRIIA+ALSP
Sbjct: 481 LALSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSP 540
Query: 613 VTPCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPKPVFARIEQA 662
VTP LSWRIYAQLG+SKDQF+AATWNETKWGGLKAGQVMAPP PVFARIEQA
Sbjct: 541 VTPRLSWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQA 585
BLAST of Sgr030463 vs. NCBI nr
Match:
XP_022133974.1 (methionine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia])
HSP 1 Score: 1068.5 bits (2762), Expect = 2.2e-308
Identity = 542/596 (90.94%), Postives = 558/596 (93.62%), Query Frame = 0
Query: 73 MGARINTSIQSSLWLLNPLQ-----NLKAT-HLRTRLHFSRTLFSSPSSSSSSSSSPSSK 132
M ARIN+SIQSSLWLLNPLQ NL +LRTRL+F R L SPS S+S SSK
Sbjct: 1 MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSAS-----SSK 60
Query: 133 RAIFCTSASNSNSHEREPAADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKK 192
RAIFCT ASN NSHE A DPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKK
Sbjct: 61 RAIFCTYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKK 120
Query: 193 VIFITGTDEHGEKIATAAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEA 252
VIFITGTDEHGEKIATAAAAQGS PSEHCDAISEAYK+LWKDLDVAYDKFIRTT+PKHEA
Sbjct: 121 VIFITGTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEA 180
Query: 253 IVKEFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFF 312
IVKEFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKE+NYFF
Sbjct: 181 IVKEFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFF 240
Query: 313 ALSKYQRSLEETLLQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQT 372
ALSKYQ LEETL QNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQT
Sbjct: 241 ALSKYQGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQT 300
Query: 373 IYVWFDALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGL 432
IYVWFDALLGYISALSENG+Q LQR VASGWPASLHLIGKDILRFHAVYWPAMLMSAGL
Sbjct: 301 IYVWFDALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGL 360
Query: 433 KLPKMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAED 492
KLPKMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFL+EVEFGSDGDYAED
Sbjct: 361 KLPKMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAED 420
Query: 493 RFINIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNH 552
RFINIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEG +FKDKVEELV+KARNH
Sbjct: 421 RFINIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNH 480
Query: 553 YEELSLSSACEAVLEIGNAGNSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVA 612
YE LSLSSACEAVLEIGNAGNSY+DE+APWSLFKQGGASS AAAKDL+LILEAMRIIAVA
Sbjct: 481 YEGLSLSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVA 540
Query: 613 LSPVTPCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPKPVFARIEQAS 663
LSPVTPCL WRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPP PVFARIEQ++
Sbjct: 541 LSPVTPCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQST 591
BLAST of Sgr030463 vs. NCBI nr
Match:
KAG7033041.1 (Methionine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1066.2 bits (2756), Expect = 1.1e-307
Identity = 536/592 (90.54%), Postives = 555/592 (93.75%), Query Frame = 0
Query: 73 MGARINTSIQSSLWLLNPLQ---NLKATHLRTRLHFSRTLFSSPSSSSSSSSSPSSKRAI 132
M ARINTSIQ+SLWLLNPLQ NLKATHLR R +F + L S+PSS SSS SS SSKRAI
Sbjct: 1 MAARINTSIQNSLWLLNPLQSSRNLKATHLRIRFNFYQNLLSAPSSCSSSCSSSSSKRAI 60
Query: 133 FCTSASNSNSHEREPAADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 192
FC+SASN NSH+ A DPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF
Sbjct: 61 FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120
Query: 193 ITGTDEHGEKIATAAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVK 252
ITGTDEHGEKIATAAAAQGS PSEHCD ISEAYK+LWKDLD+AYDKFIRTT+PKHE IVK
Sbjct: 121 ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK 180
Query: 253 EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFFALS 312
EFYS VLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKE+NYFFALS
Sbjct: 181 EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240
Query: 313 KYQRSLEETLLQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYV 372
KYQR LEETL QNPGFVQPAFRLNEVQSWI+SGLKDFSISRASVEWGIPVPNDQKQTIYV
Sbjct: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV 300
Query: 373 WFDALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 432
WFDALLGYISALSEN EQP LQ VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP
Sbjct: 301 WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360
Query: 433 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFI 492
KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSD VRYFFLREVEFGSDGDYAEDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI 420
Query: 493 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEE 552
NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDS+AAA G AFK+KVEELV KARNHYEE
Sbjct: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE 480
Query: 553 LSLSSACEAVLEIGNAGNSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSP 612
LSLSSACEAVLEIGNAGNSY+DERAPWSLFKQGGASS AAAKDL+LILEAMRIIA+ALSP
Sbjct: 481 LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP 540
Query: 613 VTPCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPKPVFARIEQA 662
VTP LSWRIY QLG+ +DQF AATWNETKWGGLKAGQVMAPP PVFARIEQA
Sbjct: 541 VTPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQA 592
BLAST of Sgr030463 vs. NCBI nr
Match:
XP_038891037.1 (methionine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida])
HSP 1 Score: 1064.7 bits (2752), Expect = 3.3e-307
Identity = 531/592 (89.70%), Postives = 557/592 (94.09%), Query Frame = 0
Query: 73 MGARINTSIQSSLWLLNPLQ---NLKATHLRTRLHFSRTLFSSPSSSSSSSSSPSSKRAI 132
M ARINTSIQ+SLWLLNP Q NLK THLRTRLHF + L S PSSSS SSKRAI
Sbjct: 1 MAARINTSIQTSLWLLNPCQSSLNLKPTHLRTRLHFYQNLLSLPSSSS------SSKRAI 60
Query: 133 FCTSASNSNSHEREPAADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 192
FCT+A++ NSH+ A +PFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF
Sbjct: 61 FCTAATSFNSHDNGAATNPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120
Query: 193 ITGTDEHGEKIATAAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVK 252
ITGTDEHGEKIATAAA QGS PSEHCD ISEAYK+LWKDLDVAYD+FIRTT+PKHEAIVK
Sbjct: 121 ITGTDEHGEKIATAAATQGSSPSEHCDVISEAYKTLWKDLDVAYDRFIRTTDPKHEAIVK 180
Query: 253 EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFFALS 312
EFYS+VLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASR+E+NYFFALS
Sbjct: 181 EFYSRVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRREDNYFFALS 240
Query: 313 KYQRSLEETLLQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYV 372
KYQR LEETL QNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND+KQTIYV
Sbjct: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYV 300
Query: 373 WFDALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 432
WFDALLGYISALSENGEQP L+R+VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP
Sbjct: 301 WFDALLGYISALSENGEQPNLERVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360
Query: 433 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFI 492
KMVFGHGFLTKDGMKMGKSLGNTLEPNELV KFGSDAVRYFFLREVEFGSDGDYAEDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVGKFGSDAVRYFFLREVEFGSDGDYAEDRFI 420
Query: 493 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEE 552
NIVNAHLANTIGNLLNRTLGLL+KNCQSTLVVDSTAAA G FKDKVEEL+VKARNHYEE
Sbjct: 421 NIVNAHLANTIGNLLNRTLGLLQKNCQSTLVVDSTAAAGGNVFKDKVEELIVKARNHYEE 480
Query: 553 LSLSSACEAVLEIGNAGNSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSP 612
L+LSSACEAVLEIGNAGNSY+DERAPWSLFKQGGAS+ AAAKDL+LILEAMRIIA+ALSP
Sbjct: 481 LALSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASTDAAAKDLVLILEAMRIIAIALSP 540
Query: 613 VTPCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPKPVFARIEQA 662
VTP LSWRIYAQLG+S+DQF AATWNETKWGGLKAGQVMAPP PVFARIEQA
Sbjct: 541 VTPRLSWRIYAQLGYSEDQFNAATWNETKWGGLKAGQVMAPPNPVFARIEQA 586
BLAST of Sgr030463 vs. NCBI nr
Match:
XP_022960433.1 (methionine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata])
HSP 1 Score: 1062.4 bits (2746), Expect = 1.6e-306
Identity = 535/592 (90.37%), Postives = 554/592 (93.58%), Query Frame = 0
Query: 73 MGARINTSIQSSLWLLNPLQ---NLKATHLRTRLHFSRTLFSSPSSSSSSSSSPSSKRAI 132
M ARINTSIQ+SLWLLNPLQ NLKATHLRTR +F + L S+PSS SSS SSKRAI
Sbjct: 1 MAARINTSIQNSLWLLNPLQSSRNLKATHLRTRFNFYQNLLSAPSSCSSS----SSKRAI 60
Query: 133 FCTSASNSNSHEREPAADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 192
FC+SASN NSH+ A DPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF
Sbjct: 61 FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120
Query: 193 ITGTDEHGEKIATAAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVK 252
ITGTDEHGEKIATAAAAQGS PSEHCD ISEAYK+LWKDLD+AYDKFIRTT+PKHE IVK
Sbjct: 121 ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK 180
Query: 253 EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFFALS 312
EFYS VLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKE+NYFFALS
Sbjct: 181 EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240
Query: 313 KYQRSLEETLLQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYV 372
KYQR LEETL QNPGFVQPAFRLNEVQSWI+SGLKDFSISRASVEWGIPVPNDQKQTIYV
Sbjct: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV 300
Query: 373 WFDALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 432
WFDALLGYISALSEN EQP LQ VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP
Sbjct: 301 WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360
Query: 433 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFI 492
KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSD VRYFFLREVEFGSDGDYAEDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI 420
Query: 493 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEE 552
NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDS+AAA G AFK+KVEELV KARNHYEE
Sbjct: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE 480
Query: 553 LSLSSACEAVLEIGNAGNSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSP 612
LSLSSACEAVLEIGNAGNSY+DERAPWSLFKQGGASS AAAKDL+LILEAMRIIA+ALSP
Sbjct: 481 LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP 540
Query: 613 VTPCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPKPVFARIEQA 662
VTP LSWRIY QLG+ +DQF AATWNETKWGGLKAGQVMAPP PVFARIEQA
Sbjct: 541 VTPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQA 588
BLAST of Sgr030463 vs. ExPASy Swiss-Prot
Match:
Q9M2T9 (Methionine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OVA1 PE=2 SV=1)
HSP 1 Score: 890.2 bits (2299), Expect = 1.4e-257
Identity = 441/588 (75.00%), Postives = 500/588 (85.03%), Query Frame = 0
Query: 73 MGARINTSIQSSLWLLNPLQNLKATHLRTR-LHFSRTLFSSPSSSSSSSSSPSSKRAIFC 132
M ARINTS+ ++L L P T L T+ F R F S S KRA++C
Sbjct: 1 MAARINTSLHNALSFLKPFN----TPLNTKPFSFRRNSFRFSKKLPYYSQFSSGKRALYC 60
Query: 133 TSASNSNSHEREPAADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 192
TS+S ++ + + FV+TTPLYYVNA PHMGSAYTTIAAD+IARFQRLLGKKVIFIT
Sbjct: 61 TSSSQESTVDE---GETFVLTTPLYYVNAPPHMGSAYTTIAADSIARFQRLLGKKVIFIT 120
Query: 193 GTDEHGEKIATAAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVKEF 252
GTDEHGEKIAT+AAA G +P EHCD IS++Y++LWKDLD+AYDKFIRTT+PKHEAIVKEF
Sbjct: 121 GTDEHGEKIATSAAANGRNPPEHCDLISQSYRTLWKDLDIAYDKFIRTTDPKHEAIVKEF 180
Query: 253 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFFALSKY 312
Y++V ANGDIYRADYEGLYCVNCEEYKDEKELL NNCCP+H PC +RKE+NYFFALSKY
Sbjct: 181 YARVFANGDIYRADYEGLYCVNCEEYKDEKELLENNCCPVHQMPCVARKEDNYFFALSKY 240
Query: 313 QRSLEETLLQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 372
Q+ LE+ L QNP FVQP++RLNEVQSWI+SGL+DFSISRA V+WGIPVP+D KQTIYVWF
Sbjct: 241 QKPLEDILAQNPRFVQPSYRLNEVQSWIKSGLRDFSISRALVDWGIPVPDDDKQTIYVWF 300
Query: 373 DALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 432
DALLGYISAL+E+ +Q L+ V+ GWPASLHLIGKDILRFHAVYWPAMLMSAGL+LPKM
Sbjct: 301 DALLGYISALTEDNKQQNLETAVSFGWPASLHLIGKDILRFHAVYWPAMLMSAGLELPKM 360
Query: 433 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINI 492
VFGHGFLTKDGMKMGKSLGNTLEP ELVQKFG DAVRYFFLREVEFG+DGDY+EDRFI I
Sbjct: 361 VFGHGFLTKDGMKMGKSLGNTLEPFELVQKFGPDAVRYFFLREVEFGNDGDYSEDRFIKI 420
Query: 493 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEELS 552
VNAHLANTIGNLLNRTLGLLKKNC+STLVVDST AAEG KD VE+LV KAR +YE LS
Sbjct: 421 VNAHLANTIGNLLNRTLGLLKKNCESTLVVDSTVAAEGVPLKDTVEKLVEKARTNYENLS 480
Query: 553 LSSACEAVLEIGNAGNSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSPVT 612
LSSACEAVLEIGNAGN+Y+D+RAPW LFKQGG S+ AAKDL++ILE MR+IAVALSPV
Sbjct: 481 LSSACEAVLEIGNAGNTYMDQRAPWFLFKQGGVSAEEAAKDLVIILEVMRVIAVALSPVA 540
Query: 613 PCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPKPVFARIE 660
PCLS RIY+QLG+++DQF + TW++TKWGGLK GQVM PVFARIE
Sbjct: 541 PCLSLRIYSQLGYTEDQFNSITWSDTKWGGLKGGQVMEQASPVFARIE 581
BLAST of Sgr030463 vs. ExPASy Swiss-Prot
Match:
Q55729 (Methionine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=metG PE=3 SV=1)
HSP 1 Score: 563.1 bits (1450), Expect = 4.1e-159
Identity = 277/521 (53.17%), Postives = 357/521 (68.52%), Query Frame = 0
Query: 149 FVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQG 208
F +TTPLYYVN PH+GSAYTT+ AD +ARF+RL G V+ ITGTDEHG+KI A AQ
Sbjct: 9 FSVTTPLYYVNDVPHLGSAYTTVVADTLARFKRLQGYDVLMITGTDEHGQKIQRTAEAQE 68
Query: 209 SDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVKEFYSKVLANGDIYRADYEG 268
DP HCD +K LW+ L++ YD+F RTT+P+H AIVK+F+ +V GDIY A +G
Sbjct: 69 LDPQTHCDRTVVKFKELWRSLNILYDRFSRTTDPRHLAIVKDFFQRVWDKGDIYLAQQQG 128
Query: 269 LYCVNCEEYKDEKELLANNCCPIHLKPCAS-RKENNYFFALSKYQRSLEETLLQNPGFVQ 328
YCV CEE+K++++LL +N CP+H A R E NYFF LS+YQ LEE Q P F+Q
Sbjct: 129 WYCVACEEFKEKRDLLEDNHCPLHPNRKAEWRDEENYFFRLSRYQHPLEELYAQRPEFIQ 188
Query: 329 PAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDALLGYISALSENGEQ 388
P+ R NEV +++ GL+DFSISR +++WG P+PND TIYVWFDALLGY++AL + E+
Sbjct: 189 PSSRRNEVLNFVAQGLQDFSISRVNLDWGFPLPNDPNHTIYVWFDALLGYVTALLDEDEE 248
Query: 389 PKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMGK 448
P L + WP +LHLIGKDILRFHAVYWPAMLMSA L +P VFGHGFLTKDG KMGK
Sbjct: 249 PNLTNALVKWWPINLHLIGKDILRFHAVYWPAMLMSAELAIPAQVFGHGFLTKDGQKMGK 308
Query: 449 SLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRT 508
SLGNT++P +L+ ++G DA RY+FL+E+EFG DGD+ E RF+N++NA LAN +GNLLNRT
Sbjct: 309 SLGNTVDPLDLINRYGEDAFRYYFLKEIEFGKDGDFNEQRFVNVLNADLANDLGNLLNRT 368
Query: 509 LGLLKKNCQST-LVVDSTAAAEGTAFKDKVEELVVKARNHYEELSLSSACEAVLEIGNAG 568
LG++KK CQ V +T A K L + + YE LS + ACEA+ + AG
Sbjct: 369 LGMVKKYCQGQGPQVMATDLAPDNPLKALGSHLGEEVSSAYERLSFTDACEAIFTLVRAG 428
Query: 569 NSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSPVTPCLSWRIYAQLGFSK 628
N YID+ APW LFKQG S L +LE++R+ A LSP+ P LS +IY QLGF+
Sbjct: 429 NKYIDDMAPWKLFKQG--SQKEVEDVLYSVLESIRLSAYLLSPIVPRLSTKIYQQLGFTW 488
Query: 629 D-------QFEAATWN-ETKWGGLKAGQVMAPPKPVFARIE 660
D +A +N WG L A Q + P +P+F ++E
Sbjct: 489 DFDQWRSPLEQAEEFNRHQSWGQLGANQNLPPAQPIFTKLE 527
BLAST of Sgr030463 vs. ExPASy Swiss-Prot
Match:
Q8Z068 (Methionine--tRNA ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=metG PE=3 SV=1)
HSP 1 Score: 556.6 bits (1433), Expect = 3.8e-157
Identity = 279/523 (53.35%), Postives = 364/523 (69.60%), Query Frame = 0
Query: 145 AADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAA 204
A F +TTPLYYVN PH+GSAYTT+AADA+ARFQ+LLG+ V+ ITGTDEHG+KI +A
Sbjct: 7 AEKTFALTTPLYYVNDVPHIGSAYTTMAADAVARFQKLLGRDVLLITGTDEHGQKIQRSA 66
Query: 205 AAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVKEFYSKVLANGDIYRA 264
+ G P E CD I ++ SLW+ L++ YD+F RTT +H+AIV EF+++V GDIY+
Sbjct: 67 ESLGKAPQEFCDEIVPSFMSLWRLLNIQYDRFSRTTAVRHKAIVDEFFARVWEAGDIYQG 126
Query: 265 DYEGLYCVNCEEYKDEKELLANNCCPIHL-KPCASRKENNYFFALSKYQRSLEETLLQNP 324
+G YCV+CEE+K+E+ELL N CPIH+ K R E NYFF LSKYQ LEE +P
Sbjct: 127 QQKGWYCVSCEEFKEERELLEGNRCPIHVNKEVEWRDEQNYFFRLSKYQTQLEEFYQSHP 186
Query: 325 GFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDALLGYISALSE 384
F+QP R NEV +++ GL+DFSISR +++WG PVPND K T+YVWFDALL Y++AL +
Sbjct: 187 DFIQPESRRNEVLNFVSQGLQDFSISRVNLDWGFPVPNDPKHTLYVWFDALLAYVTALLD 246
Query: 385 NGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGM 444
++P L+ + WP +LHLIGKDILRFHAVYWPAML+SAGL LP VFGHGFLTKDG
Sbjct: 247 PEDEPTLENALGKWWPINLHLIGKDILRFHAVYWPAMLLSAGLPLPDRVFGHGFLTKDGQ 306
Query: 445 KMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTIGNL 504
KMGKSLGNT++P LVQ++GSDAVRY+FL+E+EFG DGD+ E RFI+++NA LAN +GNL
Sbjct: 307 KMGKSLGNTVDPVGLVQQYGSDAVRYYFLKEIEFGKDGDFNEVRFIHVLNADLANDLGNL 366
Query: 505 LNRTLGLLKKNC--QSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEELSLSSACEAVLE 564
LNRTL ++KK C + + + AE T K +L K + YE L+ + AC VL
Sbjct: 367 LNRTLNMVKKYCANYALSITNEDIPAENT-LKALGVDLGAKVKQAYEVLAFNQACGVVLS 426
Query: 565 IGNAGNSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSPVTPCLSWRIYAQ 624
+ A N +ID++APWSL+KQ L +LE++R+ A LSPV P +S IY Q
Sbjct: 427 LVQASNKFIDDQAPWSLYKQERQQEVETV--LYTVLESVRLAAYLLSPVIPNISSNIYQQ 486
Query: 625 LGFS---KDQFEAATWN-ETKWGGLKAGQVMAPPKPVFARIEQ 661
LGF +Q + + +WG L Q + P+P+F RIEQ
Sbjct: 487 LGFGINFNEQTDITPFAIHAQWGLLSNKQQLGQPQPIFKRIEQ 526
BLAST of Sgr030463 vs. ExPASy Swiss-Prot
Match:
P59081 (Methionine--tRNA ligase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=metG PE=3 SV=1)
HSP 1 Score: 538.1 bits (1385), Expect = 1.4e-151
Identity = 265/518 (51.16%), Postives = 352/518 (67.95%), Query Frame = 0
Query: 149 FVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQG 208
F +TTPLYYVNA PH+GSAYTTIAAD +ARF RL G +V FITGTDEHG+KI A +G
Sbjct: 5 FSLTTPLYYVNALPHIGSAYTTIAADVLARFYRLQGYQVRFITGTDEHGQKIERTAQQRG 64
Query: 209 SDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVKEFYSKVLANGDIYRADYEG 268
P HCD I+ +++LW+ L++ YD+F RTT+P+H AIV EF+ +V NGDIY +G
Sbjct: 65 LSPQAHCDEIAAGFQALWQQLNIHYDRFSRTTSPRHHAIVNEFFQRVWDNGDIYLGQQQG 124
Query: 269 LYCVNCEEYKDEKELLANNCCPIHLKPCAS-RKENNYFFALSKYQRSLEETLLQNPGFVQ 328
YCV CEE+K+E+ELL CPIH+ R E NYFF LSKYQ++L + ++P FVQ
Sbjct: 125 WYCVECEEFKEERELLEGRRCPIHVNRTVEWRDERNYFFRLSKYQQALLDHYAEHPDFVQ 184
Query: 329 PAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDALLGYISALSENGEQ 388
P R NEV S+I GL+DFSISR ++ WG PVP D +QT+YVWFDALLGY++AL E ++
Sbjct: 185 PPSRRNEVLSFIERGLQDFSISRVNLAWGFPVPTDPEQTLYVWFDALLGYVTALLEPEDE 244
Query: 389 PKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMGK 448
P L + + WP +LH+IGKDILRFH + WPAMLMSAGL LP+ +F HGFLTKDG KMGK
Sbjct: 245 PTLANALKTWWPINLHIIGKDILRFHGISWPAMLMSAGLPLPEQIFVHGFLTKDGQKMGK 304
Query: 449 SLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRT 508
SLGNTL+P LV ++G+DAVRY+F++EVEFG DGD++E RF+ I+NA LAN +GNLLNRT
Sbjct: 305 SLGNTLDPFALVAQYGADAVRYYFMKEVEFGRDGDFSETRFVTILNADLANDLGNLLNRT 364
Query: 509 LGLLKKNCQSTLV-VDSTAAAEGTAFKDKVEELVVKARNHYEELSLSSACEAVLEIGNAG 568
L + K + V A + E L Y +L+ C+ L + AG
Sbjct: 365 LKMAWKYTDGKVPNVQGAAIPREHPLRQLAEHLSQTYGQGYRQLAFHEVCQQALTLARAG 424
Query: 569 NSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSPVTPCLSWRIYAQLGFSK 628
N ++DE APW ++ G +AA A+ L +LE++R++A L+P+ P LS IY QLG+S
Sbjct: 425 NKFLDEEAPWKRYRAG--ETAAVAEILYCVLESVRLVAYVLAPIIPQLSEAIYGQLGYS- 484
Query: 629 DQFEAATW------NETKWGGLKAGQVMAPPKPVFARI 659
+F + + +WG L A Q +A P+P+F ++
Sbjct: 485 IRFNGSIAPDLLGDRQAQWGVLPASQPLANPEPIFQKL 519
BLAST of Sgr030463 vs. ExPASy Swiss-Prot
Match:
O67298 (Methionine--tRNA ligase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=metG PE=1 SV=1)
HSP 1 Score: 429.1 bits (1102), Expect = 9.1e-119
Identity = 217/466 (46.57%), Postives = 290/466 (62.23%), Query Frame = 0
Query: 149 FVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQG 208
F +TTP+YYVN PH+G AYTTIAAD IAR+ RL V F+TGTDEHG KI A G
Sbjct: 7 FYVTTPIYYVNDVPHLGHAYTTIAADTIARYYRLRDYDVFFLTGTDEHGLKIQKKAEELG 66
Query: 209 SDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVKEFYSKVLANGDIYRADYEG 268
P E D +E +K LW+ L + Y KFIRTT+P H V++ + + GDIY +YEG
Sbjct: 67 ISPKELVDRNAERFKKLWEFLKIEYTKFIRTTDPYHVKFVQKVFEECYKRGDIYLGEYEG 126
Query: 269 LYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFFALSKYQRSLEETLLQNPGFVQP 328
YCV CEE+K E EL ++ CPIH K C KE +YFF LSKYQ L E +NP F+QP
Sbjct: 127 WYCVGCEEFKSEAELAEDHTCPIHQKKCEYIKEPSYFFRLSKYQDKLLELYEKNPEFIQP 186
Query: 329 AFRLNEVQSWIRSGLKDFSIS--RASVEWGIPVPNDQKQTIYVWFDALLGYISALSENGE 388
+R NE+ S+++ GLKD S++ R+ V+WGIPVP D + TIYVWFDAL YISAL +
Sbjct: 187 DYRRNEIISFVKQGLKDLSVTRPRSRVKWGIPVPFDPEHTIYVWFDALFNYISALEDK-- 246
Query: 389 QPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMG 448
V WPA LHL+GKDILRFH VYWPA LMS G +LPK VF HG+ T +G KM
Sbjct: 247 -------VEIYWPADLHLVGKDILRFHTVYWPAFLMSLGYELPKKVFAHGWWTVEGKKMS 306
Query: 449 KSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTIGNLLNR 508
K+LGN ++P E+VQ++G D VRYF LREV FG DGD+++ +N +N LAN IGNL +R
Sbjct: 307 KTLGNVVDPYEVVQEYGLDEVRYFLLREVPFGQDGDFSKKAILNRINGELANEIGNLYSR 366
Query: 509 TLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEELSLSSACEAVLEIGNAG 568
+ + K + + A + +E + N+ E+++ A E +L+ +
Sbjct: 367 VVNMAHK-----FLGGEVSGARDEEYAKIAQESIKNYENYMEKVNFYKAIEEILKFTSYL 426
Query: 569 NSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSPVTP 613
N Y+DE+ PW+L K+ K L +++ + ++ L P+TP
Sbjct: 427 NKYVDEKQPWALNKE--RKKEELQKVLYALVDGLFVLTHLLYPITP 456
BLAST of Sgr030463 vs. ExPASy TrEMBL
Match:
A0A0A0KQV7 (Methionine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_5G512910 PE=3 SV=1)
HSP 1 Score: 1072.0 bits (2771), Expect = 9.9e-310
Identity = 536/592 (90.54%), Postives = 555/592 (93.75%), Query Frame = 0
Query: 73 MGARINTSIQSSLWLLNPLQ---NLKATHLRTRLHFSRTLFSSPSSSSSSSSSPSSKRAI 132
M ARINTSIQ+SLWLLNP NLK TH RTRLHF + L S PS S SSKRAI
Sbjct: 1 MAARINTSIQTSLWLLNPFHSSLNLKPTHFRTRLHFCQNLLSFPSHS-------SSKRAI 60
Query: 133 FCTSASNSNSHEREPAADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 192
FCTS SN NSH+ A DPFVITTPLYYVNAAPHMGSAYTTIAADA+ARFQRLLGKKVIF
Sbjct: 61 FCTSTSNFNSHDSGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIF 120
Query: 193 ITGTDEHGEKIATAAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVK 252
ITGTDEHGEKIATAAA QGS PSEHCD ISEAYK+LWKDLDVAYDKFIRTT+PKHEAIVK
Sbjct: 121 ITGTDEHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVK 180
Query: 253 EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFFALS 312
EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKE+NYFFALS
Sbjct: 181 EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240
Query: 313 KYQRSLEETLLQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYV 372
KYQR LEETL QNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND+KQTIYV
Sbjct: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYV 300
Query: 373 WFDALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 432
WFDALLGYISALSENGEQP LQ +VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP
Sbjct: 301 WFDALLGYISALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360
Query: 433 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFI 492
KMVFGHGFLTKDGMKMGKSLGNTLEPNELV+KFGSDAVRYFFLREVEFGSDGDYAEDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFI 420
Query: 493 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEE 552
NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAA+G AFKDKVEELVVKARN+YEE
Sbjct: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEE 480
Query: 553 LSLSSACEAVLEIGNAGNSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSP 612
L+LSSACEAVLEIGNAGNSY+DERAPWSLFKQGGASS AAAKDL+LILEAMRIIA+ALSP
Sbjct: 481 LALSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSP 540
Query: 613 VTPCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPKPVFARIEQA 662
VTP LSWRIYAQLG+SKDQF+AATWNETKWGGLKAGQVMAPP PVFARIEQA
Sbjct: 541 VTPRLSWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQA 585
BLAST of Sgr030463 vs. ExPASy TrEMBL
Match:
A0A6J1BXG8 (Methionine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111006379 PE=3 SV=1)
HSP 1 Score: 1068.5 bits (2762), Expect = 1.1e-308
Identity = 542/596 (90.94%), Postives = 558/596 (93.62%), Query Frame = 0
Query: 73 MGARINTSIQSSLWLLNPLQ-----NLKAT-HLRTRLHFSRTLFSSPSSSSSSSSSPSSK 132
M ARIN+SIQSSLWLLNPLQ NL +LRTRL+F R L SPS S+S SSK
Sbjct: 1 MAARINSSIQSSLWLLNPLQSSLNLNLNLNLNLRTRLNFYRNLLPSPSYSAS-----SSK 60
Query: 133 RAIFCTSASNSNSHEREPAADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKK 192
RAIFCT ASN NSHE A DPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKK
Sbjct: 61 RAIFCTYASNCNSHESGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKK 120
Query: 193 VIFITGTDEHGEKIATAAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEA 252
VIFITGTDEHGEKIATAAAAQGS PSEHCDAISEAYK+LWKDLDVAYDKFIRTT+PKHEA
Sbjct: 121 VIFITGTDEHGEKIATAAAAQGSAPSEHCDAISEAYKTLWKDLDVAYDKFIRTTDPKHEA 180
Query: 253 IVKEFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFF 312
IVKEFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKE+NYFF
Sbjct: 181 IVKEFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKEDNYFF 240
Query: 313 ALSKYQRSLEETLLQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQT 372
ALSKYQ LEETL QNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQT
Sbjct: 241 ALSKYQGLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQT 300
Query: 373 IYVWFDALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGL 432
IYVWFDALLGYISALSENG+Q LQR VASGWPASLHLIGKDILRFHAVYWPAMLMSAGL
Sbjct: 301 IYVWFDALLGYISALSENGQQQNLQRAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGL 360
Query: 433 KLPKMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAED 492
KLPKMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFL+EVEFGSDGDYAED
Sbjct: 361 KLPKMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLQEVEFGSDGDYAED 420
Query: 493 RFINIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNH 552
RFINIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEG +FKDKVEELV+KARNH
Sbjct: 421 RFINIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGNSFKDKVEELVLKARNH 480
Query: 553 YEELSLSSACEAVLEIGNAGNSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVA 612
YE LSLSSACEAVLEIGNAGNSY+DE+APWSLFKQGGASS AAAKDL+LILEAMRIIAVA
Sbjct: 481 YEGLSLSSACEAVLEIGNAGNSYMDEQAPWSLFKQGGASSDAAAKDLVLILEAMRIIAVA 540
Query: 613 LSPVTPCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPKPVFARIEQAS 663
LSPVTPCL WRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPP PVFARIEQ++
Sbjct: 541 LSPVTPCLGWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPNPVFARIEQST 591
BLAST of Sgr030463 vs. ExPASy TrEMBL
Match:
A0A6J1H8X3 (Methionine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111461165 PE=3 SV=1)
HSP 1 Score: 1062.4 bits (2746), Expect = 7.9e-307
Identity = 535/592 (90.37%), Postives = 554/592 (93.58%), Query Frame = 0
Query: 73 MGARINTSIQSSLWLLNPLQ---NLKATHLRTRLHFSRTLFSSPSSSSSSSSSPSSKRAI 132
M ARINTSIQ+SLWLLNPLQ NLKATHLRTR +F + L S+PSS SSS SSKRAI
Sbjct: 1 MAARINTSIQNSLWLLNPLQSSRNLKATHLRTRFNFYQNLLSAPSSCSSS----SSKRAI 60
Query: 133 FCTSASNSNSHEREPAADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 192
FC+SASN NSH+ A DPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF
Sbjct: 61 FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120
Query: 193 ITGTDEHGEKIATAAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVK 252
ITGTDEHGEKIATAAAAQGS PSEHCD ISEAYK+LWKDLD+AYDKFIRTT+PKHE IVK
Sbjct: 121 ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK 180
Query: 253 EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFFALS 312
EFYS VLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKE+NYFFALS
Sbjct: 181 EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240
Query: 313 KYQRSLEETLLQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYV 372
KYQR LEETL QNPGFVQPAFRLNEVQSWI+SGLKDFSISRASVEWGIPVPNDQKQTIYV
Sbjct: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV 300
Query: 373 WFDALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 432
WFDALLGYISALSEN EQP LQ VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP
Sbjct: 301 WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360
Query: 433 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFI 492
KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSD VRYFFLREVEFGSDGDYAEDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI 420
Query: 493 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEE 552
NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDS+AAA G AFK+KVEELV KARNHYEE
Sbjct: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE 480
Query: 553 LSLSSACEAVLEIGNAGNSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSP 612
LSLSSACEAVLEIGNAGNSY+DERAPWSLFKQGGASS AAAKDL+LILEAMRIIA+ALSP
Sbjct: 481 LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP 540
Query: 613 VTPCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPKPVFARIEQA 662
VTP LSWRIY QLG+ +DQF AATWNETKWGGLKAGQVMAPP PVFARIEQA
Sbjct: 541 VTPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQA 588
BLAST of Sgr030463 vs. ExPASy TrEMBL
Match:
A0A5D3C1K0 (Methionine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G003330 PE=3 SV=1)
HSP 1 Score: 1059.7 bits (2739), Expect = 5.1e-306
Identity = 532/592 (89.86%), Postives = 552/592 (93.24%), Query Frame = 0
Query: 73 MGARINTSIQSSLWLLNPLQ---NLKATHLRTRLHFSRTLFSSPSSSSSSSSSPSSKRAI 132
M ARINTSIQ+SL LLNP NLK TH RTRLHF + L S S S SSKRAI
Sbjct: 1 MAARINTSIQTSLSLLNPFHNSLNLKPTHFRTRLHFYQNLLSFASYS-------SSKRAI 60
Query: 133 FCTSASNSNSHEREPAADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 192
FCT+ SN NSH+ A DPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVI
Sbjct: 61 FCTTTSNFNSHDTGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIL 120
Query: 193 ITGTDEHGEKIATAAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVK 252
ITGTDEHGEKIATAAA QGS PSEHCD ISEAYK+LWKDLDVAYDKFIRTT+PKHEAIVK
Sbjct: 121 ITGTDEHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVK 180
Query: 253 EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFFALS 312
EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKE+NYFFALS
Sbjct: 181 EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240
Query: 313 KYQRSLEETLLQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYV 372
KYQR LEETL QNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND+KQTIYV
Sbjct: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDKKQTIYV 300
Query: 373 WFDALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 432
WFDALLGYISALSENGEQP L ++VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP
Sbjct: 301 WFDALLGYISALSENGEQPNLHKVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360
Query: 433 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFI 492
KMVFGHGFLTKDGMKMGKSLGNTLEPNELV+KFGSDAVRYFFLREVEFGSDGDYAEDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFI 420
Query: 493 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEE 552
NIVNAHLANTIGNLLNRTLGLLKKNCQSTL+VDSTAAA+G AFKDKVEELVVKARNHYEE
Sbjct: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLLVDSTAAADGNAFKDKVEELVVKARNHYEE 480
Query: 553 LSLSSACEAVLEIGNAGNSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSP 612
L+LSSACEAVLEIGNAGNSY+DERAPWSLFKQGGASS AAAKDL+LILEAMRIIA+ALSP
Sbjct: 481 LALSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSP 540
Query: 613 VTPCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPKPVFARIEQA 662
VTP LSWRIYAQLG+SKDQF+AATWNETKWGGLKAGQVMAPP PVFARIEQA
Sbjct: 541 VTPRLSWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQA 585
BLAST of Sgr030463 vs. ExPASy TrEMBL
Match:
A0A1S3CJS7 (Methionine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103501636 PE=3 SV=1)
HSP 1 Score: 1058.9 bits (2737), Expect = 8.7e-306
Identity = 532/592 (89.86%), Postives = 551/592 (93.07%), Query Frame = 0
Query: 73 MGARINTSIQSSLWLLNPLQ---NLKATHLRTRLHFSRTLFSSPSSSSSSSSSPSSKRAI 132
M ARINTSIQ+SL LLNP NLK TH RTRLHF + L S S S SSKRAI
Sbjct: 1 MAARINTSIQTSLSLLNPFHNSLNLKPTHFRTRLHFYQNLLSFASYS-------SSKRAI 60
Query: 133 FCTSASNSNSHEREPAADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 192
FCT+ SN NSH+ A DPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVI
Sbjct: 61 FCTTTSNFNSHDTGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIL 120
Query: 193 ITGTDEHGEKIATAAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVK 252
ITGTDEHGEKIATAAA QGS PSEHCD ISEAYK+LWKDLDVAYDKFIRTT+PKHEAIVK
Sbjct: 121 ITGTDEHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVK 180
Query: 253 EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFFALS 312
EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLA+NCCPIHLKPCASRKE+NYFFALS
Sbjct: 181 EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240
Query: 313 KYQRSLEETLLQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYV 372
KYQR LEETL QNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPND KQTIYV
Sbjct: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDXKQTIYV 300
Query: 373 WFDALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 432
WFDALLGYISALSENGEQP L ++VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP
Sbjct: 301 WFDALLGYISALSENGEQPNLHKVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360
Query: 433 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFI 492
KMVFGHGFLTKDGMKMGKSLGNTLEPNELV+KFGSDAVRYFFLREVEFGSDGDYAEDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFI 420
Query: 493 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEE 552
NIVNAHLANTIGNLLNRTLGLLKKNCQSTL+VDSTAAA+G AFKDKVEELVVKARNHYEE
Sbjct: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLLVDSTAAADGNAFKDKVEELVVKARNHYEE 480
Query: 553 LSLSSACEAVLEIGNAGNSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSP 612
L+LSSACEAVLEIGNAGNSY+DERAPWSLFKQGGASS AAAKDL+LILEAMRIIA+ALSP
Sbjct: 481 LALSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSP 540
Query: 613 VTPCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPKPVFARIEQA 662
VTP LSWRIYAQLG+SKDQF+AATWNETKWGGLKAGQVMAPP PVFARIEQA
Sbjct: 541 VTPRLSWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQA 585
BLAST of Sgr030463 vs. TAIR 10
Match:
AT3G55400.1 (methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) )
HSP 1 Score: 890.2 bits (2299), Expect = 1.0e-258
Identity = 441/588 (75.00%), Postives = 500/588 (85.03%), Query Frame = 0
Query: 73 MGARINTSIQSSLWLLNPLQNLKATHLRTR-LHFSRTLFSSPSSSSSSSSSPSSKRAIFC 132
M ARINTS+ ++L L P T L T+ F R F S S KRA++C
Sbjct: 1 MAARINTSLHNALSFLKPFN----TPLNTKPFSFRRNSFRFSKKLPYYSQFSSGKRALYC 60
Query: 133 TSASNSNSHEREPAADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 192
TS+S ++ + + FV+TTPLYYVNA PHMGSAYTTIAAD+IARFQRLLGKKVIFIT
Sbjct: 61 TSSSQESTVDE---GETFVLTTPLYYVNAPPHMGSAYTTIAADSIARFQRLLGKKVIFIT 120
Query: 193 GTDEHGEKIATAAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVKEF 252
GTDEHGEKIAT+AAA G +P EHCD IS++Y++LWKDLD+AYDKFIRTT+PKHEAIVKEF
Sbjct: 121 GTDEHGEKIATSAAANGRNPPEHCDLISQSYRTLWKDLDIAYDKFIRTTDPKHEAIVKEF 180
Query: 253 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFFALSKY 312
Y++V ANGDIYRADYEGLYCVNCEEYKDEKELL NNCCP+H PC +RKE+NYFFALSKY
Sbjct: 181 YARVFANGDIYRADYEGLYCVNCEEYKDEKELLENNCCPVHQMPCVARKEDNYFFALSKY 240
Query: 313 QRSLEETLLQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 372
Q+ LE+ L QNP FVQP++RLNEVQSWI+SGL+DFSISRA V+WGIPVP+D KQTIYVWF
Sbjct: 241 QKPLEDILAQNPRFVQPSYRLNEVQSWIKSGLRDFSISRALVDWGIPVPDDDKQTIYVWF 300
Query: 373 DALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 432
DALLGYISAL+E+ +Q L+ V+ GWPASLHLIGKDILRFHAVYWPAMLMSAGL+LPKM
Sbjct: 301 DALLGYISALTEDNKQQNLETAVSFGWPASLHLIGKDILRFHAVYWPAMLMSAGLELPKM 360
Query: 433 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINI 492
VFGHGFLTKDGMKMGKSLGNTLEP ELVQKFG DAVRYFFLREVEFG+DGDY+EDRFI I
Sbjct: 361 VFGHGFLTKDGMKMGKSLGNTLEPFELVQKFGPDAVRYFFLREVEFGNDGDYSEDRFIKI 420
Query: 493 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEELS 552
VNAHLANTIGNLLNRTLGLLKKNC+STLVVDST AAEG KD VE+LV KAR +YE LS
Sbjct: 421 VNAHLANTIGNLLNRTLGLLKKNCESTLVVDSTVAAEGVPLKDTVEKLVEKARTNYENLS 480
Query: 553 LSSACEAVLEIGNAGNSYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSPVT 612
LSSACEAVLEIGNAGN+Y+D+RAPW LFKQGG S+ AAKDL++ILE MR+IAVALSPV
Sbjct: 481 LSSACEAVLEIGNAGNTYMDQRAPWFLFKQGGVSAEEAAKDLVIILEVMRVIAVALSPVA 540
Query: 613 PCLSWRIYAQLGFSKDQFEAATWNETKWGGLKAGQVMAPPKPVFARIE 660
PCLS RIY+QLG+++DQF + TW++TKWGGLK GQVM PVFARIE
Sbjct: 541 PCLSLRIYSQLGYTEDQFNSITWSDTKWGGLKGGQVMEQASPVFARIE 581
BLAST of Sgr030463 vs. TAIR 10
Match:
AT3G55400.2 (methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) )
HSP 1 Score: 788.9 bits (2036), Expect = 3.2e-228
Identity = 394/522 (75.48%), Postives = 443/522 (84.87%), Query Frame = 0
Query: 73 MGARINTSIQSSLWLLNPLQNLKATHLRTR-LHFSRTLFSSPSSSSSSSSSPSSKRAIFC 132
M ARINTS+ ++L L P T L T+ F R F S S KRA++C
Sbjct: 1 MAARINTSLHNALSFLKPFN----TPLNTKPFSFRRNSFRFSKKLPYYSQFSSGKRALYC 60
Query: 133 TSASNSNSHEREPAADPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFIT 192
TS+S ++ + + FV+TTPLYYVNA PHMGSAYTTIAAD+IARFQRLLGKKVIFIT
Sbjct: 61 TSSSQESTVDE---GETFVLTTPLYYVNAPPHMGSAYTTIAADSIARFQRLLGKKVIFIT 120
Query: 193 GTDEHGEKIATAAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVKEF 252
GTDEHGEKIAT+AAA G +P EHCD IS++Y++LWKDLD+AYDKFIRTT+PKHEAIVKEF
Sbjct: 121 GTDEHGEKIATSAAANGRNPPEHCDLISQSYRTLWKDLDIAYDKFIRTTDPKHEAIVKEF 180
Query: 253 YSKVLANGDIYRADYEGLYCVNCEEYKDEKELLANNCCPIHLKPCASRKENNYFFALSKY 312
Y++V ANGDIYRADYEGLYCVNCEEYKDEKELL NNCCP+H PC +RKE+NYFFALSKY
Sbjct: 181 YARVFANGDIYRADYEGLYCVNCEEYKDEKELLENNCCPVHQMPCVARKEDNYFFALSKY 240
Query: 313 QRSLEETLLQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDQKQTIYVWF 372
Q+ LE+ L QNP FVQP++RLNEVQSWI+SGL+DFSISRA V+WGIPVP+D KQTIYVWF
Sbjct: 241 QKPLEDILAQNPRFVQPSYRLNEVQSWIKSGLRDFSISRALVDWGIPVPDDDKQTIYVWF 300
Query: 373 DALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKM 432
DALLGYISAL+E+ +Q L+ V+ GWPASLHLIGKDILRFHAVYWPAMLMSAGL+LPKM
Sbjct: 301 DALLGYISALTEDNKQQNLETAVSFGWPASLHLIGKDILRFHAVYWPAMLMSAGLELPKM 360
Query: 433 VFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINI 492
VFGHGFLTKDGMKMGKSLGNTLEP ELVQKFG DAVRYFFLREVEFG+DGDY+EDRFI I
Sbjct: 361 VFGHGFLTKDGMKMGKSLGNTLEPFELVQKFGPDAVRYFFLREVEFGNDGDYSEDRFIKI 420
Query: 493 VNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEELS 552
VNAHLANTIGNLLNRTLGLLKKNC+STLVVDST AAEG KD VE+LV KAR +YE LS
Sbjct: 421 VNAHLANTIGNLLNRTLGLLKKNCESTLVVDSTVAAEGVPLKDTVEKLVEKARTNYENLS 480
Query: 553 LSSACEAVLEIGNAGNSYIDERAPWSLFKQGGASSAAAAKDL 594
LSSACEAVLEIGNAGN+Y+D+RAPW LFKQGG S+ AAK L
Sbjct: 481 LSSACEAVLEIGNAGNTYMDQRAPWFLFKQGGVSAEEAAKVL 515
BLAST of Sgr030463 vs. TAIR 10
Match:
AT4G13780.1 (methionine--tRNA ligase, putative / methionyl-tRNA synthetase, putative / MetRS, putative )
HSP 1 Score: 161.4 bits (407), Expect = 2.5e-39
Identity = 144/583 (24.70%), Postives = 244/583 (41.85%), Query Frame = 0
Query: 144 PAADPFVITTPLYYVNAAPHMGSAY-TTIAADAIARFQRLLGKKVIFITGTDEHGEKIAT 203
P +IT+ L YVN PH+G+ ++AD AR+ RL G I+I GTDE+G T
Sbjct: 14 PGKRNILITSALPYVNNVPHLGNIIGCVLSADVYARYCRLRGYNAIYICGTDEYGTATET 73
Query: 204 AAAAQGSDPSEHCDAISEAYKSLWKDLDVAYDKFIRTTNPKHEAIVKEFYSKVLANGDIY 263
A + P E CD +K ++ +++DKF RT+ P+ + + ++K+ N +
Sbjct: 74 KALEENCTPKEICDKYHAIHKEVYDWFGISFDKFGRTSTPEQTEVCQAIFNKLWDNKWLS 133
Query: 264 RADYEGLYCVNCEEYKDEKELLANNCCPIH----------------------------LK 323
+ LYC C+++ ++ L CP K
Sbjct: 134 ENTMQQLYCDTCKKFLADR--LVEGSCPFEGCNYDSARGDQCEKCGKLLNPTELKDPKCK 193
Query: 324 PCAS----RKENNYFFALSKYQRSLEETLLQNPGFVQPAFRLNEVQ---SWIRSGLKDFS 383
C + R ++ F L + LE + + V ++ N +Q +W+R GL+
Sbjct: 194 VCQNTPRIRDTDHLFIELPLLKDRLEAYIKKTS--VTGSWSQNAIQTTNAWLRDGLRQRC 253
Query: 384 ISRASVEWGIPVPND--QKQTIYVWFDALLGYISALSENGEQPKLQRIVASGW------P 443
I+R ++WG+PVP++ + + YVWFDA +GY+S S S W P
Sbjct: 254 ITR-DLKWGVPVPHEKYKDKVFYVWFDAPIGYVSITS----------CYTSEWEKWWKNP 313
Query: 444 ASLHL---IGKDILRFHAVYWPAMLMSA--GLKLPKMVFGHGFLTKDGMKMGKSLGNTLE 503
++ L +GKD + FH V +P+ + L K + +L + K KS G +
Sbjct: 314 ENVELYQFMGKDNVPFHTVMFPSTQLGTEENWTLMKTISVTEYLNYEDGKFSKSKGVGVF 373
Query: 504 PNELVQ-KFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRTLGLLKK 563
N++ + RY+ L SD ++ +N L + +GN +NR L + K
Sbjct: 374 GNDVKDTNIPVEVWRYYLLTNRPEVSDTSFSWTDLQAKLNGELLSNLGNFVNRVLSFIAK 433
Query: 564 --NCQSTLVVDSTAAAEG----TAFKDKVEELVVKARNHYEELSLSSACEAVLEIGNAGN 623
N V+ AE + +KVE+ V + E++ L + + I + GN
Sbjct: 434 PDNAGYGSVIPDAHDAESHSLTKSLAEKVEKFVAEYVEAMEKVKLKQGLKTAMLISSEGN 493
Query: 624 SYIDERAPWSLFKQGGASSAAAAKDLILILEAMRIIAVALSPVTPCLSWRIYAQLG---- 666
Y+ W L+K+ A + + + ++A L P P S ++ QL
Sbjct: 494 YYLQASQFWKLYKEDKPLCAVVIRTAAGL---VHLLAQLLEPFMPSFSCEVFKQLNLPPQ 553
BLAST of Sgr030463 vs. TAIR 10
Match:
AT5G16715.1 (ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases )
HSP 1 Score: 47.8 bits (112), Expect = 4.1e-05
Identity = 62/257 (24.12%), Postives = 109/257 (42.41%), Query Frame = 0
Query: 398 WPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMV-FGHGFL-----TKDGMKMGKSLGN 457
+P ++ G DIL F W A ++ G++ V F H +L G KM KSLGN
Sbjct: 550 YPTNMLETGHDILFF----WVARMVMMGIEFTGTVPFSHVYLHGLIRDSQGRKMSKSLGN 609
Query: 458 TLEPNELVQKFGSDAVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTI---GNLLNRTL 517
++P + ++ FG+DA+R F + G D + + +R N N + G + +L
Sbjct: 610 VIDPLDTIKDFGTDALR-FTIALGTAGQDLNLSTERL--TANKAFTNKLWNAGKFVLHSL 669
Query: 518 GLLKKNCQSTLVVDSTAAAEGTAFKDKVEELVVKARNHYEELSLSSACEAVLEIGNAGNS 577
L ++D E T + E ++ H S++++ E L G+ G
Sbjct: 670 PSLSDTSAWENLLDLKLDKEETLLSLPLPECWAVSKLHILIDSVTASYEK-LFFGDVGRE 729
Query: 578 YID----ERAPW-------SLFKQGGASSAAAAKDLILILEAMRIIAVALSPVTPCLSWR 635
D + A W L+ GG S + A++ ++L + I L P P ++
Sbjct: 730 TYDFFWSDFADWYIEASKSRLYGSGGNSVSLASQAVLLYV--FENILKLLHPFMPFVTED 789
BLAST of Sgr030463 vs. TAIR 10
Match:
AT5G49030.1 (tRNA synthetase class I (I, L, M and V) family protein )
HSP 1 Score: 47.0 bits (110), Expect = 6.9e-05
Identity = 76/313 (24.28%), Postives = 126/313 (40.26%), Query Frame = 0
Query: 366 TIYVWFDALLGYISALSENGEQPKLQRIVASGWPASLHLIGKDILRFHAVYWPAMLMSA- 425
T+ VWFD+ + L G++ L +PA ++L G D H ++ + L+++
Sbjct: 652 TMDVWFDSGSSWAGVL---GKREGL------SFPADVYLEGTD---QHRGWFQSSLLTSI 711
Query: 426 ---GLKLPKMVFGHGF-LTKDGMKMGKSLGNTLEPNELVQ---------KFGSDAVRYFF 485
G V HGF L + GMKM KSLGN ++P +++ +G+D +R +
Sbjct: 712 ATQGKAPYSAVITHGFVLDEKGMKMSKSLGNVVDPRLVIEGGKNSKDAPAYGADVMRLWV 771
Query: 486 LREVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAAEGTA 545
S DY D I + I L TL L N VD+ +
Sbjct: 772 -------SSVDYTGDVLIGPQILRQMSDIYRKLRGTLRYLLGNLHDWR-VDNAVPYQDLP 831
Query: 546 FKD-----KVEELVVKARNHYEELSLSSACEAV--LEIGNAGNSYIDERAPWSLFKQGGA 605
D ++E +V + YE + + I + N Y D A L+ G +
Sbjct: 832 IIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVDLSNFYFD-IAKDRLYTGGTS 891
Query: 606 SSAAAAKDLILILEAMRIIAVALSPVTPCLSWRIYAQLGF---SKDQFEAATWNETKWGG 655
S + +L + I+ V ++P+ P L+ ++ L F ++D A E KW
Sbjct: 892 SFTRRSCQTVLSTHLLSILRV-IAPIVPHLAEDVWQNLPFEYRNEDGSAAEFVFELKWPT 942
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_004142742.1 | 2.0e-309 | 90.54 | methionine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN51284... | [more] |
XP_022133974.1 | 2.2e-308 | 90.94 | methionine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia] | [more] |
KAG7033041.1 | 1.1e-307 | 90.54 | Methionine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyros... | [more] |
XP_038891037.1 | 3.3e-307 | 89.70 | methionine--tRNA ligase, chloroplastic/mitochondrial [Benincasa hispida] | [more] |
XP_022960433.1 | 1.6e-306 | 90.37 | methionine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata] | [more] |
Match Name | E-value | Identity | Description | |
Q9M2T9 | 1.4e-257 | 75.00 | Methionine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=... | [more] |
Q55729 | 4.1e-159 | 53.17 | Methionine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=11117... | [more] |
Q8Z068 | 3.8e-157 | 53.35 | Methionine--tRNA ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) ... | [more] |
P59081 | 1.4e-151 | 51.16 | Methionine--tRNA ligase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221... | [more] |
O67298 | 9.1e-119 | 46.57 | Methionine--tRNA ligase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=metG PE=1 ... | [more] |
Match Name | E-value | Identity | Description | |
A0A0A0KQV7 | 9.9e-310 | 90.54 | Methionine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_5G512910 PE=3 SV=1 | [more] |
A0A6J1BXG8 | 1.1e-308 | 90.94 | Methionine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111006379 PE=3 SV=1 | [more] |
A0A6J1H8X3 | 7.9e-307 | 90.37 | Methionine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111461165 PE=3 SV=1 | [more] |
A0A5D3C1K0 | 5.1e-306 | 89.86 | Methionine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... | [more] |
A0A1S3CJS7 | 8.7e-306 | 89.86 | Methionine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103501636 PE=3 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
AT3G55400.1 | 1.0e-258 | 75.00 | methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) | [more] |
AT3G55400.2 | 3.2e-228 | 75.48 | methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) | [more] |
AT4G13780.1 | 2.5e-39 | 24.70 | methionine--tRNA ligase, putative / methionyl-tRNA synthetase, putative / MetRS,... | [more] |
AT5G16715.1 | 4.1e-05 | 24.12 | ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP bi... | [more] |
AT5G49030.1 | 6.9e-05 | 24.28 | tRNA synthetase class I (I, L, M and V) family protein | [more] |