Sgr028756 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr028756 vs. NCBI nr
Match: XP_023552657.1 (uncharacterized protein LOC111810237 [Cucurbita pepo subsp. pepo] >XP_023552666.1 uncharacterized protein LOC111810237 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 224.9 bits (572), Expect = 6.5e-55
Identity = 117/140 (83.57%), Postives = 123/140 (87.86%), Query Frame = 0

Query: 1   MGKSLPSATRLQQFAKVVVSSKKPQAAKATAKPKTRIRVSTPETPNSGSVRVISEPLKPK 60
           M KSLP A+RLQQFAKVVVSSKKPQ+  A  KPK  IRVS PETP S SVRVISEP +PK
Sbjct: 1   MAKSLPLASRLQQFAKVVVSSKKPQSPTARQKPK--IRVSPPETPISDSVRVISEPAQPK 60

Query: 61  K-MEEKNRVPLADVVSDCVKRWFQDTLKEANAGDTAMQVLVGQMFCSGYGIPKDTQKGLA 120
           K ME KNR+PLADVVSDC KRWFQDTLKEANAGDTAMQVLVGQMFCSGYG+PKDT+KGLA
Sbjct: 61  KNMEVKNRIPLADVVSDCAKRWFQDTLKEANAGDTAMQVLVGQMFCSGYGVPKDTRKGLA 120

Query: 121 WINRASKTQASVWKVSDRHP 140
           WINRASK QASVWK SDRHP
Sbjct: 121 WINRASKYQASVWKASDRHP 138

BLAST of Sgr028756 vs. NCBI nr
Match: KAG7030637.1 (hypothetical protein SDJN02_04674 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 223.8 bits (569), Expect = 1.4e-54
Identity = 116/140 (82.86%), Postives = 122/140 (87.14%), Query Frame = 0

Query: 1   MGKSLPSATRLQQFAKVVVSSKKPQAAKATAKPKTRIRVSTPETPNSGSVRVISEPLKPK 60
           M KS P A+RLQQFAKVVVSSKKPQ+  A  KPK  IRVS PETP S SVRVISEP +PK
Sbjct: 1   MAKSFPLASRLQQFAKVVVSSKKPQSPTARQKPK--IRVSPPETPISDSVRVISEPAQPK 60

Query: 61  K-MEEKNRVPLADVVSDCVKRWFQDTLKEANAGDTAMQVLVGQMFCSGYGIPKDTQKGLA 120
           K ME KNR+PLADVVSDC KRWFQDTLKEANAGDTAMQVLVGQMFCSGYG+PKDT+KGLA
Sbjct: 61  KNMEVKNRIPLADVVSDCAKRWFQDTLKEANAGDTAMQVLVGQMFCSGYGVPKDTRKGLA 120

Query: 121 WINRASKTQASVWKVSDRHP 140
           WINRASK QASVWK SDRHP
Sbjct: 121 WINRASKCQASVWKASDRHP 138

BLAST of Sgr028756 vs. NCBI nr
Match: XP_022941871.1 (uncharacterized protein LOC111447103 [Cucurbita moschata] >XP_022941872.1 uncharacterized protein LOC111447103 [Cucurbita moschata] >KAG6599959.1 hypothetical protein SDJN03_05192, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 223.4 bits (568), Expect = 1.9e-54
Identity = 116/140 (82.86%), Postives = 122/140 (87.14%), Query Frame = 0

Query: 1   MGKSLPSATRLQQFAKVVVSSKKPQAAKATAKPKTRIRVSTPETPNSGSVRVISEPLKPK 60
           M KS P A+RLQQFAKVVVSSKKPQ+  A  KPK  IRVS PETP S SVRVISEP +PK
Sbjct: 1   MAKSFPLASRLQQFAKVVVSSKKPQSPTARQKPK--IRVSPPETPISDSVRVISEPAQPK 60

Query: 61  K-MEEKNRVPLADVVSDCVKRWFQDTLKEANAGDTAMQVLVGQMFCSGYGIPKDTQKGLA 120
           K ME KNR+PLADVVSDC KRWFQDTLKEANAGDTAMQVLVGQMFCSGYG+PKDT+KGLA
Sbjct: 61  KNMEVKNRIPLADVVSDCAKRWFQDTLKEANAGDTAMQVLVGQMFCSGYGVPKDTRKGLA 120

Query: 121 WINRASKTQASVWKVSDRHP 140
           WINRASK QASVWK SDRHP
Sbjct: 121 WINRASKYQASVWKASDRHP 138

BLAST of Sgr028756 vs. NCBI nr
Match: XP_022996650.1 (uncharacterized protein LOC111491826 [Cucurbita maxima] >XP_022996657.1 uncharacterized protein LOC111491826 [Cucurbita maxima])

HSP 1 Score: 218.0 bits (554), Expect = 7.9e-53
Identity = 115/141 (81.56%), Postives = 122/141 (86.52%), Query Frame = 0

Query: 1   MGKSLPSATRLQQFAKVVVSSKKPQAAKATAKPKTRIRVSTPETPNSGSVRVISEPLKPK 60
           M KSLP A+RLQQFAKVVVSSKKPQ+     KPK  IRVS PETP S SVRVISEP +PK
Sbjct: 1   MAKSLPLASRLQQFAKVVVSSKKPQSPTPRQKPK--IRVSPPETPISDSVRVISEPAQPK 60

Query: 61  K-MEEKNRVPLADVVSDCVKRWFQDTLKEANAGDTAMQVLVGQMFCSGYGIPKDTQKGLA 120
           K ME KNR+PLADVVSDC KRWFQDTLKEANAGDTAMQVLVGQMFCSGYG+PKDT+KGLA
Sbjct: 61  KNMEVKNRIPLADVVSDCAKRWFQDTLKEANAGDTAMQVLVGQMFCSGYGVPKDTRKGLA 120

Query: 121 WINRASKTQASVWKVSDRHPG 141
           WI+RASK QASV K SDRHPG
Sbjct: 121 WISRASKYQASVRKASDRHPG 139

BLAST of Sgr028756 vs. NCBI nr
Match: KAG6600897.1 (hypothetical protein SDJN03_06130, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 214.5 bits (545), Expect = 8.7e-52
Identity = 113/140 (80.71%), Postives = 121/140 (86.43%), Query Frame = 0

Query: 1   MGKSLPSATRLQQFAKVVVSSKKPQAAKATAKPKTRIRVSTPETPNSGSVRVISEPLKPK 60
           M KSLPSAT LQQFAK+ V SKKPQ+  A  KPK+RIRVS  ETP   +VRVISEP K K
Sbjct: 1   MAKSLPSATHLQQFAKIAVFSKKPQS--AAPKPKSRIRVSPSETPIPDTVRVISEP-KKK 60

Query: 61  KMEEKNRVPLADVVSDCVKRWFQDTLKEANAGDTAMQVLVGQMFCSGYGIPKDTQKGLAW 120
            MEEKNRVPLADVVSDCVKRWFQDTLKEA+AGD AMQVLVGQMFCSGYG+PKDT+KGLAW
Sbjct: 61  NMEEKNRVPLADVVSDCVKRWFQDTLKEASAGDPAMQVLVGQMFCSGYGVPKDTKKGLAW 120

Query: 121 INRASKTQASVWKVSDRHPG 141
           INRASK QASV +VSDRHPG
Sbjct: 121 INRASKGQASVGEVSDRHPG 137

BLAST of Sgr028756 vs. ExPASy TrEMBL
Match: A0A6J1FNN7 (uncharacterized protein LOC111447103 OS=Cucurbita moschata OX=3662 GN=LOC111447103 PE=4 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 9.1e-55
Identity = 116/140 (82.86%), Postives = 122/140 (87.14%), Query Frame = 0

Query: 1   MGKSLPSATRLQQFAKVVVSSKKPQAAKATAKPKTRIRVSTPETPNSGSVRVISEPLKPK 60
           M KS P A+RLQQFAKVVVSSKKPQ+  A  KPK  IRVS PETP S SVRVISEP +PK
Sbjct: 1   MAKSFPLASRLQQFAKVVVSSKKPQSPTARQKPK--IRVSPPETPISDSVRVISEPAQPK 60

Query: 61  K-MEEKNRVPLADVVSDCVKRWFQDTLKEANAGDTAMQVLVGQMFCSGYGIPKDTQKGLA 120
           K ME KNR+PLADVVSDC KRWFQDTLKEANAGDTAMQVLVGQMFCSGYG+PKDT+KGLA
Sbjct: 61  KNMEVKNRIPLADVVSDCAKRWFQDTLKEANAGDTAMQVLVGQMFCSGYGVPKDTRKGLA 120

Query: 121 WINRASKTQASVWKVSDRHP 140
           WINRASK QASVWK SDRHP
Sbjct: 121 WINRASKYQASVWKASDRHP 138

BLAST of Sgr028756 vs. ExPASy TrEMBL
Match: A0A6J1K2L5 (uncharacterized protein LOC111491826 OS=Cucurbita maxima OX=3661 GN=LOC111491826 PE=4 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 3.8e-53
Identity = 115/141 (81.56%), Postives = 122/141 (86.52%), Query Frame = 0

Query: 1   MGKSLPSATRLQQFAKVVVSSKKPQAAKATAKPKTRIRVSTPETPNSGSVRVISEPLKPK 60
           M KSLP A+RLQQFAKVVVSSKKPQ+     KPK  IRVS PETP S SVRVISEP +PK
Sbjct: 1   MAKSLPLASRLQQFAKVVVSSKKPQSPTPRQKPK--IRVSPPETPISDSVRVISEPAQPK 60

Query: 61  K-MEEKNRVPLADVVSDCVKRWFQDTLKEANAGDTAMQVLVGQMFCSGYGIPKDTQKGLA 120
           K ME KNR+PLADVVSDC KRWFQDTLKEANAGDTAMQVLVGQMFCSGYG+PKDT+KGLA
Sbjct: 61  KNMEVKNRIPLADVVSDCAKRWFQDTLKEANAGDTAMQVLVGQMFCSGYGVPKDTRKGLA 120

Query: 121 WINRASKTQASVWKVSDRHPG 141
           WI+RASK QASV K SDRHPG
Sbjct: 121 WISRASKYQASVRKASDRHPG 139

BLAST of Sgr028756 vs. ExPASy TrEMBL
Match: A0A6J1GZI5 (uncharacterized protein LOC111458931 OS=Cucurbita moschata OX=3662 GN=LOC111458931 PE=4 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 1.6e-51
Identity = 112/140 (80.00%), Postives = 120/140 (85.71%), Query Frame = 0

Query: 1   MGKSLPSATRLQQFAKVVVSSKKPQAAKATAKPKTRIRVSTPETPNSGSVRVISEPLKPK 60
           M KSLPSAT LQQFAK+ V SKKPQ+  A  KPK+RIRVS  ETP   +VRVISEP K K
Sbjct: 1   MAKSLPSATHLQQFAKIAVFSKKPQS--AAPKPKSRIRVSPSETPIPDTVRVISEP-KKK 60

Query: 61  KMEEKNRVPLADVVSDCVKRWFQDTLKEANAGDTAMQVLVGQMFCSGYGIPKDTQKGLAW 120
            MEEKNRVPLADVVSDCVKRWFQDTLKEA+AGD AMQVLVGQMFCSGYG+PKDT+KGLAW
Sbjct: 61  NMEEKNRVPLADVVSDCVKRWFQDTLKEASAGDPAMQVLVGQMFCSGYGVPKDTKKGLAW 120

Query: 121 INRASKTQASVWKVSDRHPG 141
           INRASK QA V +VSDRHPG
Sbjct: 121 INRASKGQAPVGEVSDRHPG 137

BLAST of Sgr028756 vs. ExPASy TrEMBL
Match: A0A6J1JUC7 (uncharacterized protein LOC111488439 OS=Cucurbita maxima OX=3661 GN=LOC111488439 PE=4 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 1.4e-50
Identity = 110/140 (78.57%), Postives = 120/140 (85.71%), Query Frame = 0

Query: 1   MGKSLPSATRLQQFAKVVVSSKKPQAAKATAKPKTRIRVSTPETPNSGSVRVISEPLKPK 60
           M KSLPSA+ LQQFAK+ V SKKPQ+  A  KPK+RIRVS  ETP   +VRVISEP K K
Sbjct: 1   MAKSLPSASHLQQFAKIAVFSKKPQS--AAPKPKSRIRVSPSETPIPDTVRVISEP-KKK 60

Query: 61  KMEEKNRVPLADVVSDCVKRWFQDTLKEANAGDTAMQVLVGQMFCSGYGIPKDTQKGLAW 120
            MEE+NRVPLADVVSDCVKRWFQDTLKEA+AGD AMQVLVGQMFCSGYG+PKDT+KGLAW
Sbjct: 61  NMEEQNRVPLADVVSDCVKRWFQDTLKEASAGDPAMQVLVGQMFCSGYGVPKDTKKGLAW 120

Query: 121 INRASKTQASVWKVSDRHPG 141
           INRASK QA V +VSDRHPG
Sbjct: 121 INRASKGQAPVGEVSDRHPG 137

BLAST of Sgr028756 vs. ExPASy TrEMBL
Match: A0A0A0KM25 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G218760 PE=4 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 7.5e-49
Identity = 108/142 (76.06%), Postives = 115/142 (80.99%), Query Frame = 0

Query: 1   MGKSLPSATRLQQFAKVVVSSKKPQAAKATAKPKTRIRVSTPETPNSGSVRVISEPLKPK 60
           M KSLPS  R QQFAK+V SSK PQ    +   K+RIR S  ETP SGSVRVISEP K K
Sbjct: 1   MAKSLPSPARFQQFAKLVFSSKNPQ----SQPKKSRIRASPSETPISGSVRVISEPNKNK 60

Query: 61  KM--EEKNRVPLADVVSDCVKRWFQDTLKEANAGDTAMQVLVGQMFCSGYGIPKDTQKGL 120
            M  EEKNR PLADVVSDCVKRWFQDTLKEA AGDT+MQVLVGQMFCSGYG+PK+T+KGL
Sbjct: 61  YMEEEEKNRTPLADVVSDCVKRWFQDTLKEAKAGDTSMQVLVGQMFCSGYGVPKNTKKGL 120

Query: 121 AWINRASKTQASVWKVSDRHPG 141
           AWI RASK QASVWK SDRHPG
Sbjct: 121 AWIYRASKYQASVWKASDRHPG 138

BLAST of Sgr028756 vs. TAIR 10
Match: AT5G05360.2 (unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G38450.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 127.5 bits (319), Expect = 1.3e-29
Identity = 71/153 (46.41%), Postives = 93/153 (60.78%), Query Frame = 0

Query: 1   MGKSLPSATRLQQFAKVVVSSKKPQAAKATAKPKTRI------RVSTPETPNSGSVRVI- 60
           MGKS+    R  +FA  + S    +  + +  P+ ++      R +T     S  V++  
Sbjct: 1   MGKSM---VRFAEFAIRLSSENPTRPHRPSPSPRNKVFVKKTTRDTTSHLDYSNLVKLEK 60

Query: 61  ------SEPLKPKKMEEKNRVPLADVVSDCVKRWFQDTLKEANAGDTAMQVLVGQMFCSG 120
                 S P      +  NRVPLA VV DCV+RWFQDTLKEA +GD  MQVLVGQM+CSG
Sbjct: 61  AGSHSGSNPAPASGSDPINRVPLAQVVEDCVRRWFQDTLKEAKSGDVGMQVLVGQMYCSG 120

Query: 121 YGIPKDTQKGLAWINRASKTQASVWKVSDRHPG 141
           YGIPKD  KG AWIN+AS+T++S W+VSD+ PG
Sbjct: 121 YGIPKDENKGRAWINKASRTRSSAWQVSDKPPG 150

BLAST of Sgr028756 vs. TAIR 10
Match: AT5G05360.1 (unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G38450.1); Has 84 Blast hits to 84 proteins in 24 species: Archae - 0; Bacteria - 8; Metazoa - 0; Fungi - 0; Plants - 73; Viruses - 0; Other Eukaryotes - 3 (source: NCBI BLink). )

HSP 1 Score: 127.5 bits (319), Expect = 1.3e-29
Identity = 71/153 (46.41%), Postives = 93/153 (60.78%), Query Frame = 0

Query: 1   MGKSLPSATRLQQFAKVVVSSKKPQAAKATAKPKTRI------RVSTPETPNSGSVRVI- 60
           MGKS+    R  +FA  + S    +  + +  P+ ++      R +T     S  V++  
Sbjct: 1   MGKSM---VRFAEFAIRLSSENPTRPHRPSPSPRNKVFVKKTTRDTTSHLDYSNLVKLEK 60

Query: 61  ------SEPLKPKKMEEKNRVPLADVVSDCVKRWFQDTLKEANAGDTAMQVLVGQMFCSG 120
                 S P      +  NRVPLA VV DCV+RWFQDTLKEA +GD  MQVLVGQM+CSG
Sbjct: 61  AGSHSGSNPAPASGSDPINRVPLAQVVEDCVRRWFQDTLKEAKSGDVGMQVLVGQMYCSG 120

Query: 121 YGIPKDTQKGLAWINRASKTQASVWKVSDRHPG 141
           YGIPKD  KG AWIN+AS+T++S W+VSD+ PG
Sbjct: 121 YGIPKDENKGRAWINKASRTRSSAWQVSDKPPG 150

BLAST of Sgr028756 vs. TAIR 10
Match: AT2G38450.1 (CONTAINS InterPro DOMAIN/s: Sel1-like (InterPro:IPR006597); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G05360.1); Has 88 Blast hits to 88 proteins in 25 species: Archae - 0; Bacteria - 16; Metazoa - 0; Fungi - 0; Plants - 70; Viruses - 0; Other Eukaryotes - 2 (source: NCBI BLink). )

HSP 1 Score: 113.6 bits (283), Expect = 2.0e-25
Identity = 63/140 (45.00%), Postives = 83/140 (59.29%), Query Frame = 0

Query: 1   MGKSLPSATRLQQFAKVVVSSKKPQAAKATAKPKTRIRVSTPETPNSGSVRVISEPLKPK 60
           MGKS+P  T L+  +  V    K      ++KP   I        +S S    SE     
Sbjct: 1   MGKSIPVKTGLRGASAAVAGFIK------SSKPIRPISSMDSSDKDSSSTTTTSE----- 60

Query: 61  KMEEKNRVPLADVVSDCVKRWFQDTLKEANAGDTAMQVLVGQMFCSGYGIPKDTQKGLAW 120
               +  VPL+ VVSDC KRWF+DTL+EA AG+  MQVL+GQM+ SGYG+PKD +KG  W
Sbjct: 61  --TTRRFVPLSSVVSDCAKRWFKDTLEEAKAGNITMQVLLGQMYYSGYGVPKDARKGRLW 120

Query: 121 INRASKTQASVWKVSDRHPG 141
           I +AS+ ++SVWKV D+ PG
Sbjct: 121 ITKASRVRSSVWKVIDKRPG 127