Homology
BLAST of Sgr028675 vs. NCBI nr
Match:
XP_022147909.1 (ent-kaur-16-ene synthase, chloroplastic isoform X2 [Momordica charantia])
HSP 1 Score: 1117.4 bits (2889), Expect = 0.0e+00
Identity = 539/735 (73.33%), Postives = 625/735 (85.03%), Query Frame = 0
Query: 1 MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
MF KVELSVSAYDTAW+A+VP+PN L PLFPECINW+LDNQ PDGSWG +H H +L KA
Sbjct: 96 MFNKVELSVSAYDTAWVAMVPSPNSLNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKA 155
Query: 61 TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
+LLSTLAC L LKRW+IG EHM K LNFI+SN+ SATDEKQ SP+GFDIIFPGMIEYAK
Sbjct: 156 SLLSTLACALTLKRWNIGHEHMSKALNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKD 215
Query: 121 LELNLPLTQMNVDALIHRRELELNRSNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRKNGS 180
L+LNLPL NVDAL+ ++EL+L RSNSEG KAYLAYVSEGIG+ +DW+MVM +QRKNGS
Sbjct: 216 LDLNLPLAPANVDALLQKKELDLRRSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKNGS 275
Query: 181 LFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLGIDH 240
LFNSPSTTA AFM+ N+D C +YLHSLLQKF N+VPTIYPLD YA+LCMVD+LQ LGID
Sbjct: 276 LFNSPSTTAVAFMHGNDDGCFNYLHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGIDR 335
Query: 241 HFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDRFSD 300
HFKEEI VLD+TYR W+QGEE+I LDASTCAMAFRMLR+ GY+VSSDQL QFSED FS+
Sbjct: 336 HFKEEIRSVLDETYRCWMQGEEDIFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLFSN 395
Query: 301 CLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRLHRNMK 360
CLGGYLKD GS LELFKASQI IYP++ +LENINSWT+ FL++GLSS S+HSDR R +K
Sbjct: 396 CLGGYLKDFGSSLELFKASQIIIYPDESILENINSWTNSFLSHGLSSGSVHSDRPDRLLK 455
Query: 361 QEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMEDFNVC 420
QE I+AL+FTY+ TLERL K A+E+Y+++ +RI KT Y+CLN GHQDFL LA+EDFN+
Sbjct: 456 QEAINALEFTYSNTLERLLNKRAMENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFNIL 515
Query: 421 QCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKNGVLT 480
Q IH +E K+LERWVVEN+LDKL ARQKL YCYF+AAA +TSPEL DARLSWAKNGVLT
Sbjct: 516 QSIHHKELKELERWVVENRLDKLKFARQKLAYCYFSAAATLTSPELRDARLSWAKNGVLT 575
Query: 481 TVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKALTW 540
TVVDD FDV GSE E INL+Q VE+WDV + +YCS++VEIIF ALH TV EI +AL W
Sbjct: 576 TVVDDFFDVGGSEGELINLIQLVEKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRALPW 635
Query: 541 QGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPALYFIG 600
QGR V +V DIWL L++SMR+EAEWL NK P MD+YMENGYISFALGPIVLPAL+F+G
Sbjct: 636 QGRSVMRNVIDIWLALLKSMRKEAEWLKNKIAPPMDEYMENGYISFALGPIVLPALFFVG 695
Query: 601 PKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNRTNEE 660
PKL EEV+GS EYH LFKLMSTCGRLLND Q+ ERESSEGKLNA+SL MIN GG+ T EE
Sbjct: 696 PKLPEEVIGSCEYHKLFKLMSTCGRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTKEE 755
Query: 661 VIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSLGLMN 720
AI+ +++++RRELLRLVLQEK+ST+PRACKDLFW+MSCVVHLFYRKDDGFTS LMN
Sbjct: 756 ASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHELMN 815
Query: 721 SAKALFEEPISFDNL 735
SAKAL E+P+ D L
Sbjct: 816 SAKALLEQPMVLDEL 830
BLAST of Sgr028675 vs. NCBI nr
Match:
XP_022147908.1 (ent-kaur-16-ene synthase, chloroplastic isoform X1 [Momordica charantia])
HSP 1 Score: 1112.4 bits (2876), Expect = 0.0e+00
Identity = 539/737 (73.13%), Postives = 625/737 (84.80%), Query Frame = 0
Query: 1 MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
MF KVELSVSAYDTAW+A+VP+PN L PLFPECINW+LDNQ PDGSWG +H H +L KA
Sbjct: 96 MFNKVELSVSAYDTAWVAMVPSPNSLNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKA 155
Query: 61 TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
+LLSTLAC L LKRW+IG EHM K LNFI+SN+ SATDEKQ SP+GFDIIFPGMIEYAK
Sbjct: 156 SLLSTLACALTLKRWNIGHEHMSKALNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKD 215
Query: 121 LELNLPLTQMNVDALIHRRELELN--RSNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRKN 180
L+LNLPL NVDAL+ ++EL+L RSNSEG KAYLAYVSEGIG+ +DW+MVM +QRKN
Sbjct: 216 LDLNLPLAPANVDALLQKKELDLRSCRSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKN 275
Query: 181 GSLFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLGI 240
GSLFNSPSTTA AFM+ N+D C +YLHSLLQKF N+VPTIYPLD YA+LCMVD+LQ LGI
Sbjct: 276 GSLFNSPSTTAVAFMHGNDDGCFNYLHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGI 335
Query: 241 DHHFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDRF 300
D HFKEEI VLD+TYR W+QGEE+I LDASTCAMAFRMLR+ GY+VSSDQL QFSED F
Sbjct: 336 DRHFKEEIRSVLDETYRCWMQGEEDIFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLF 395
Query: 301 SDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRLHRN 360
S+CLGGYLKD GS LELFKASQI IYP++ +LENINSWT+ FL++GLSS S+HSDR R
Sbjct: 396 SNCLGGYLKDFGSSLELFKASQIIIYPDESILENINSWTNSFLSHGLSSGSVHSDRPDRL 455
Query: 361 MKQEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMEDFN 420
+KQE I+AL+FTY+ TLERL K A+E+Y+++ +RI KT Y+CLN GHQDFL LA+EDFN
Sbjct: 456 LKQEAINALEFTYSNTLERLLNKRAMENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFN 515
Query: 421 VCQCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKNGV 480
+ Q IH +E K+LERWVVEN+LDKL ARQKL YCYF+AAA +TSPEL DARLSWAKNGV
Sbjct: 516 ILQSIHHKELKELERWVVENRLDKLKFARQKLAYCYFSAAATLTSPELRDARLSWAKNGV 575
Query: 481 LTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKAL 540
LTTVVDD FDV GSE E INL+Q VE+WDV + +YCS++VEIIF ALH TV EI +AL
Sbjct: 576 LTTVVDDFFDVGGSEGELINLIQLVEKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRAL 635
Query: 541 TWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPALYF 600
WQGR V +V DIWL L++SMR+EAEWL NK P MD+YMENGYISFALGPIVLPAL+F
Sbjct: 636 PWQGRSVMRNVIDIWLALLKSMRKEAEWLKNKIAPPMDEYMENGYISFALGPIVLPALFF 695
Query: 601 IGPKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNRTN 660
+GPKL EEV+GS EYH LFKLMSTCGRLLND Q+ ERESSEGKLNA+SL MIN GG+ T
Sbjct: 696 VGPKLPEEVIGSCEYHKLFKLMSTCGRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTK 755
Query: 661 EEVIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSLGL 720
EE AI+ +++++RRELLRLVLQEK+ST+PRACKDLFW+MSCVVHLFYRKDDGFTS L
Sbjct: 756 EEASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHEL 815
Query: 721 MNSAKALFEEPISFDNL 735
MNSAKAL E+P+ D L
Sbjct: 816 MNSAKALLEQPMVLDEL 832
BLAST of Sgr028675 vs. NCBI nr
Match:
XP_022147910.1 (ent-kaur-16-ene synthase, chloroplastic isoform X3 [Momordica charantia])
HSP 1 Score: 1112.4 bits (2876), Expect = 0.0e+00
Identity = 539/737 (73.13%), Postives = 625/737 (84.80%), Query Frame = 0
Query: 1 MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
MF KVELSVSAYDTAW+A+VP+PN L PLFPECINW+LDNQ PDGSWG +H H +L KA
Sbjct: 83 MFNKVELSVSAYDTAWVAMVPSPNSLNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKA 142
Query: 61 TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
+LLSTLAC L LKRW+IG EHM K LNFI+SN+ SATDEKQ SP+GFDIIFPGMIEYAK
Sbjct: 143 SLLSTLACALTLKRWNIGHEHMSKALNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKD 202
Query: 121 LELNLPLTQMNVDALIHRRELELN--RSNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRKN 180
L+LNLPL NVDAL+ ++EL+L RSNSEG KAYLAYVSEGIG+ +DW+MVM +QRKN
Sbjct: 203 LDLNLPLAPANVDALLQKKELDLRSCRSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKN 262
Query: 181 GSLFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLGI 240
GSLFNSPSTTA AFM+ N+D C +YLHSLLQKF N+VPTIYPLD YA+LCMVD+LQ LGI
Sbjct: 263 GSLFNSPSTTAVAFMHGNDDGCFNYLHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGI 322
Query: 241 DHHFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDRF 300
D HFKEEI VLD+TYR W+QGEE+I LDASTCAMAFRMLR+ GY+VSSDQL QFSED F
Sbjct: 323 DRHFKEEIRSVLDETYRCWMQGEEDIFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLF 382
Query: 301 SDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRLHRN 360
S+CLGGYLKD GS LELFKASQI IYP++ +LENINSWT+ FL++GLSS S+HSDR R
Sbjct: 383 SNCLGGYLKDFGSSLELFKASQIIIYPDESILENINSWTNSFLSHGLSSGSVHSDRPDRL 442
Query: 361 MKQEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMEDFN 420
+KQE I+AL+FTY+ TLERL K A+E+Y+++ +RI KT Y+CLN GHQDFL LA+EDFN
Sbjct: 443 LKQEAINALEFTYSNTLERLLNKRAMENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFN 502
Query: 421 VCQCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKNGV 480
+ Q IH +E K+LERWVVEN+LDKL ARQKL YCYF+AAA +TSPEL DARLSWAKNGV
Sbjct: 503 ILQSIHHKELKELERWVVENRLDKLKFARQKLAYCYFSAAATLTSPELRDARLSWAKNGV 562
Query: 481 LTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKAL 540
LTTVVDD FDV GSE E INL+Q VE+WDV + +YCS++VEIIF ALH TV EI +AL
Sbjct: 563 LTTVVDDFFDVGGSEGELINLIQLVEKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRAL 622
Query: 541 TWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPALYF 600
WQGR V +V DIWL L++SMR+EAEWL NK P MD+YMENGYISFALGPIVLPAL+F
Sbjct: 623 PWQGRSVMRNVIDIWLALLKSMRKEAEWLKNKIAPPMDEYMENGYISFALGPIVLPALFF 682
Query: 601 IGPKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNRTN 660
+GPKL EEV+GS EYH LFKLMSTCGRLLND Q+ ERESSEGKLNA+SL MIN GG+ T
Sbjct: 683 VGPKLPEEVIGSCEYHKLFKLMSTCGRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTK 742
Query: 661 EEVIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSLGL 720
EE AI+ +++++RRELLRLVLQEK+ST+PRACKDLFW+MSCVVHLFYRKDDGFTS L
Sbjct: 743 EEASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHEL 802
Query: 721 MNSAKALFEEPISFDNL 735
MNSAKAL E+P+ D L
Sbjct: 803 MNSAKALLEQPMVLDEL 819
BLAST of Sgr028675 vs. NCBI nr
Match:
XP_016900364.1 (PREDICTED: ent-kaur-16-ene synthase, chloroplastic isoform X2 [Cucumis melo])
HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 530/735 (72.11%), Postives = 618/735 (84.08%), Query Frame = 0
Query: 1 MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
+F+ VELSVSAYDTAW+A+VP+PN L PLFPECINW+LD+Q PDGSWG LH H ++ KA
Sbjct: 47 LFKNVELSVSAYDTAWVAMVPSPNSLNKPLFPECINWVLDHQNPDGSWGILHDHQLVMKA 106
Query: 61 TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
TLLSTLAC+L LKRW+IG +HM K L+FI+SN+ SATDE QRSP+GFDIIFPGMIEYAK
Sbjct: 107 TLLSTLACVLTLKRWNIGHDHMSKALSFIKSNIASATDENQRSPVGFDIIFPGMIEYAKD 166
Query: 121 LELNLPLTQMNVDALIHRRELELNRSNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRKNGS 180
L LNLPL MNVDAL+ ++ELEL RSNSEG KAYLAYVSEGIGK QDWDMVM +QRKNGS
Sbjct: 167 LNLNLPLASMNVDALVQKKELEL-RSNSEGGKAYLAYVSEGIGKLQDWDMVMQYQRKNGS 226
Query: 181 LFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLGIDH 240
LF+SPSTTA AFM+ N+D C +YL S+LQKF ++VP IYPLD YA+L MVDSLQ LGID
Sbjct: 227 LFSSPSTTAVAFMHRNDDGCFNYLRSILQKFHSSVPAIYPLDIYARLHMVDSLQKLGIDR 286
Query: 241 HFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDRFSD 300
HFK+EI VLD+TYR W+QGEENI LDASTCAMAFRMLRV GY+VSSDQL QFSED F +
Sbjct: 287 HFKDEIRSVLDETYRCWMQGEENIFLDASTCAMAFRMLRVEGYDVSSDQLTQFSEDLFPN 346
Query: 301 CLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRLHRNMK 360
CLGG+LKD G+ LELFKASQI IYP++ +LENINSWTS FLN+GLS S+HSDR R +K
Sbjct: 347 CLGGHLKDFGASLELFKASQIIIYPDEFILENINSWTSRFLNHGLSGGSVHSDRTERLVK 406
Query: 361 QEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMEDFNVC 420
QE ++A +F YN+TLERL K A+E YS + +RI KT Y+CLN GHQDFL LA+EDFN
Sbjct: 407 QEAVNAFEFPYNSTLERLSNKRALESYSGDIVRISKTTYACLNFGHQDFLELAVEDFNTL 466
Query: 421 QCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKNGVLT 480
QCIHR+E K+LE+WV+ENKLDKL ARQKL YCYF+AAA +TSPEL DARLSWAKNGVLT
Sbjct: 467 QCIHRKELKELEKWVIENKLDKLKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGVLT 526
Query: 481 TVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKALTW 540
TVVDD FDV GSE+E +NL+Q VE+WD + YCS++VEIIF ALHST+ EIG KAL W
Sbjct: 527 TVVDDFFDVGGSEEELVNLIQLVEKWDASGEAGYCSKEVEIIFLALHSTICEIGEKALPW 586
Query: 541 QGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPALYFIG 600
QGR VT +V DIWL L+ SMR+EAEWL NK VPS+D+YMENGY+SFALGPIVLP LYF+G
Sbjct: 587 QGRSVTRNVIDIWLALLESMRKEAEWLKNKVVPSLDEYMENGYVSFALGPIVLPTLYFVG 646
Query: 601 PKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNRTNEE 660
PKL EE++G+ EY LFKLMST GRLLND +T +RESSEGKLNALSL++I+ GG + EE
Sbjct: 647 PKLPEEIIGNCEYQKLFKLMSTSGRLLNDTRTFDRESSEGKLNALSLYLISAGGKLSKEE 706
Query: 661 VIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSLGLMN 720
A++ D+D++RRELLRLVLQE +STIPRACKDLFW+MSCVVHLFYRKDDGFTS LMN
Sbjct: 707 ATEAMKGDVDRTRRELLRLVLQE-NSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHELMN 766
Query: 721 SAKALFEEPISFDNL 735
SAKALFE P+ D L
Sbjct: 767 SAKALFELPMVLDEL 779
BLAST of Sgr028675 vs. NCBI nr
Match:
XP_008447073.1 (PREDICTED: ent-kaur-16-ene synthase, chloroplastic isoform X1 [Cucumis melo])
HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 529/737 (71.78%), Postives = 617/737 (83.72%), Query Frame = 0
Query: 1 MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
+F+ VELSVSAYDTAW+A+VP+PN L PLFPECINW+LD+Q PDGSWG LH H ++ KA
Sbjct: 47 LFKNVELSVSAYDTAWVAMVPSPNSLNKPLFPECINWVLDHQNPDGSWGILHDHQLVMKA 106
Query: 61 TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
TLLSTLAC+L LKRW+IG +HM K L+FI+SN+ SATDE QRSP+GFDIIFPGMIEYAK
Sbjct: 107 TLLSTLACVLTLKRWNIGHDHMSKALSFIKSNIASATDENQRSPVGFDIIFPGMIEYAKD 166
Query: 121 LELNLPLTQMNVDALIHRRELELNR--SNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRKN 180
L LNLPL MNVDAL+ ++ELEL SNSEG KAYLAYVSEGIGK QDWDMVM +QRKN
Sbjct: 167 LNLNLPLASMNVDALVQKKELELRSCCSNSEGGKAYLAYVSEGIGKLQDWDMVMQYQRKN 226
Query: 181 GSLFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLGI 240
GSLF+SPSTTA AFM+ N+D C +YL S+LQKF ++VP IYPLD YA+L MVDSLQ LGI
Sbjct: 227 GSLFSSPSTTAVAFMHRNDDGCFNYLRSILQKFHSSVPAIYPLDIYARLHMVDSLQKLGI 286
Query: 241 DHHFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDRF 300
D HFK+EI VLD+TYR W+QGEENI LDASTCAMAFRMLRV GY+VSSDQL QFSED F
Sbjct: 287 DRHFKDEIRSVLDETYRCWMQGEENIFLDASTCAMAFRMLRVEGYDVSSDQLTQFSEDLF 346
Query: 301 SDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRLHRN 360
+CLGG+LKD G+ LELFKASQI IYP++ +LENINSWTS FLN+GLS S+HSDR R
Sbjct: 347 PNCLGGHLKDFGASLELFKASQIIIYPDEFILENINSWTSRFLNHGLSGGSVHSDRTERL 406
Query: 361 MKQEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMEDFN 420
+KQE ++A +F YN+TLERL K A+E YS + +RI KT Y+CLN GHQDFL LA+EDFN
Sbjct: 407 VKQEAVNAFEFPYNSTLERLSNKRALESYSGDIVRISKTTYACLNFGHQDFLELAVEDFN 466
Query: 421 VCQCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKNGV 480
QCIHR+E K+LE+WV+ENKLDKL ARQKL YCYF+AAA +TSPEL DARLSWAKNGV
Sbjct: 467 TLQCIHRKELKELEKWVIENKLDKLKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGV 526
Query: 481 LTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKAL 540
LTTVVDD FDV GSE+E +NL+Q VE+WD + YCS++VEIIF ALHST+ EIG KAL
Sbjct: 527 LTTVVDDFFDVGGSEEELVNLIQLVEKWDASGEAGYCSKEVEIIFLALHSTICEIGEKAL 586
Query: 541 TWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPALYF 600
WQGR VT +V DIWL L+ SMR+EAEWL NK VPS+D+YMENGY+SFALGPIVLP LYF
Sbjct: 587 PWQGRSVTRNVIDIWLALLESMRKEAEWLKNKVVPSLDEYMENGYVSFALGPIVLPTLYF 646
Query: 601 IGPKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNRTN 660
+GPKL EE++G+ EY LFKLMST GRLLND +T +RESSEGKLNALSL++I+ GG +
Sbjct: 647 VGPKLPEEIIGNCEYQKLFKLMSTSGRLLNDTRTFDRESSEGKLNALSLYLISAGGKLSK 706
Query: 661 EEVIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSLGL 720
EE A++ D+D++RRELLRLVLQE +STIPRACKDLFW+MSCVVHLFYRKDDGFTS L
Sbjct: 707 EEATEAMKGDVDRTRRELLRLVLQE-NSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHEL 766
Query: 721 MNSAKALFEEPISFDNL 735
MNSAKALFE P+ D L
Sbjct: 767 MNSAKALFELPMVLDEL 782
BLAST of Sgr028675 vs. ExPASy Swiss-Prot
Match:
Q39548 (Ent-kaur-16-ene synthase, chloroplastic OS=Cucurbita maxima OX=3661 PE=1 SV=1)
HSP 1 Score: 1005.4 bits (2598), Expect = 3.4e-292
Identity = 493/737 (66.89%), Postives = 592/737 (80.33%), Query Frame = 0
Query: 1 MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
+F+KVELSVSAYDTAW+A+VP+PN L PLFPECINW+LD+Q DGSWG LH +L KA
Sbjct: 51 LFDKVELSVSAYDTAWVAMVPSPNSLNQPLFPECINWVLDSQHADGSWGLLHNDQLLMKA 110
Query: 61 TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
LLSTLAC+L LKRW+IG +HM K L+FI+SN+ SATDE QRSP+GFDIIFPGMIEYAK
Sbjct: 111 NLLSTLACVLTLKRWNIGHDHMSKALDFIKSNIASATDENQRSPVGFDIIFPGMIEYAKD 170
Query: 121 LELNLPLTQMNVDALIHRRELELN--RSNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRKN 180
L LNLPL NVDAL+ ++ELEL RSNSEG KAYLAYVSEGIGK QDWDMVM +QRKN
Sbjct: 171 LNLNLPLAPTNVDALVRKKELELRSCRSNSEGGKAYLAYVSEGIGKLQDWDMVMQYQRKN 230
Query: 181 GSLFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLGI 240
GSLFNSPSTTAAAFM+ N+D C DYL SLLQKF +VPTIYPLD YA+L MVDSLQ GI
Sbjct: 231 GSLFNSPSTTAAAFMHRNDDGCFDYLRSLLQKFDGSVPTIYPLDIYARLHMVDSLQKFGI 290
Query: 241 DHHFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDRF 300
HFKEEI VLD+TYR W+QGEENI LDASTCAMAFRMLRV GY+VSSDQL QFSED F
Sbjct: 291 ARHFKEEIRSVLDETYRCWMQGEENIFLDASTCAMAFRMLRVEGYDVSSDQLTQFSEDIF 350
Query: 301 SDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRLHRN 360
+CLGGYLKD G+ LEL+KASQI +P++ VLENINSWTS FL +GLSS+S+ SDR
Sbjct: 351 PNCLGGYLKDFGASLELYKASQIITHPDESVLENINSWTSRFLKHGLSSDSVWSDRTDSV 410
Query: 361 MKQEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMEDFN 420
+KQE ++AL+F YN TLERL K A+E YS + +RI K+PY+CLN GHQDFL LA+EDFN
Sbjct: 411 VKQEAVNALEFPYNATLERLISKRAMESYSGDIVRISKSPYACLNFGHQDFLELAVEDFN 470
Query: 421 VCQCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKNGV 480
Q IH +E ++L+RWVVENKLD+L R L YCYF AAA +T PEL DAR++WA+NGV
Sbjct: 471 TLQRIHLKELEELQRWVVENKLDELKFFRLHLGYCYFAAAATLTDPELHDARIAWAQNGV 530
Query: 481 LTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKAL 540
LTTVVDD +D GSE+E NL++ VE+WD ++ YCS+ VEI+F ALHSTV EIG +AL
Sbjct: 531 LTTVVDDFYDGGGSEEELDNLIELVEKWDPDGEVGYCSKDVEIVFLALHSTVCEIGRRAL 590
Query: 541 TWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPALYF 600
WQGR V +V D WL L++ MR+EAEW +NK VPSM +YME ++SFALGPI+LP L+F
Sbjct: 591 VWQGRSVMRNVIDGWLALLKVMRKEAEWSTNKVVPSMGEYMEQAHVSFALGPIILPMLFF 650
Query: 601 IGPKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNRTN 660
+GPKLSEE++GS EY L+KLMST GRL ND+++ +RE EGKLN LSL MI+ GGN T
Sbjct: 651 VGPKLSEEMIGSCEYQKLYKLMSTAGRLKNDIRSYDRECKEGKLNILSLWMIDGGGNVTK 710
Query: 661 EEVIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSLGL 720
EE I AI+ D +++ RELL LVLQE ++TIPRACKDLFW++ +V+LFY +DDG+TS L
Sbjct: 711 EEAIEAIKGDFERAIRELLGLVLQE-NTTIPRACKDLFWKLMSIVNLFYMEDDGYTSNRL 770
Query: 721 MNSAKALFEEPISFDNL 735
MN+ KA+FE+P+ D L
Sbjct: 771 MNTVKAMFEQPMDLDAL 786
BLAST of Sgr028675 vs. ExPASy Swiss-Prot
Match:
A0A1Z3GC64 (Ent-kaurene synthase 5, chloroplastic OS=Isodon rubescens OX=587669 GN=KSL5 PE=1 SV=1)
HSP 1 Score: 823.5 bits (2126), Expect = 1.8e-237
Identity = 403/734 (54.90%), Postives = 528/734 (71.93%), Query Frame = 0
Query: 1 MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
+F K ELSVS YDTAW+A+VP+P + P FP C+NWLL+NQ DGSW H H +L K
Sbjct: 56 LFHKNELSVSTYDTAWVAMVPSPTSSEEPCFPACLNWLLENQCHDGSWARPHHHHMLKKD 115
Query: 61 TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
L STLAC+L LK+W +G+E + +GL+F+E N SAT++ Q +P+GFDIIFP M++ A+
Sbjct: 116 VLSSTLACILALKKWGVGEEQINRGLHFVELNFASATEKGQITPMGFDIIFPAMLDNARG 175
Query: 121 LELNLPLTQMNVDALIHRRELEL---NRSNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRK 180
L LNL L ++ LI++R+LEL N+SNS ++ Y A+++EG+GK QDW+ VM +QRK
Sbjct: 176 LSLNLQLEPTTLNDLIYKRDLELKRCNQSNSAEKEVYWAHIAEGMGKLQDWESVMKYQRK 235
Query: 181 NGSLFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLG 240
NGSLFN PSTTAAAF L N CL+YL L+KFG+AVP +YPLD Y+QLC VD+L+ LG
Sbjct: 236 NGSLFNCPSTTAAAFTALRNSDCLNYLRLALEKFGSAVPAVYPLDIYSQLCTVDNLERLG 295
Query: 241 IDHHFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDR 300
I +F EI VLD+TYR WLQG+E I +DASTCA+AFR LR+ GY V+SD + + ++
Sbjct: 296 ISRYFLTEIQSVLDETYRSWLQGDEEIFMDASTCALAFRTLRMNGYNVTSDPITKILQEC 355
Query: 301 FSDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNS-IHSDRLH 360
FS G + DI + LE+++AS++ +YPE+ L+ N FL LSSN I S +L
Sbjct: 356 FSSSFRGNMTDINTTLEIYRASELILYPEERDLDQHNLRLKTFLEQELSSNGFIQSCQLG 415
Query: 361 RNMKQEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMED 420
RN+ EV A+++ + ++R+ + IE+Y+++N RILKT Y N G++DFL L++ED
Sbjct: 416 RNINAEVNQAIEYPFYAIMDRMAKRKNIENYNIDNTRILKTSYRSPNFGNKDFLSLSVED 475
Query: 421 FNVCQCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKN 480
FN CQ IHR+E ++LERWV+EN+LD+L AR K YCYF+AAA I SPELSDAR+SWAKN
Sbjct: 476 FNRCQVIHREELRELERWVIENRLDELKFARSKAAYCYFSAAATIFSPELSDARMSWAKN 535
Query: 481 GVLTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNK 540
GVLTTVVDD FDV GS +E NL+Q VE WDV + CS V+IIF AL T+ EI +K
Sbjct: 536 GVLTTVVDDFFDVGGSVEELKNLIQLVELWDVDVSTQCCSPNVQIIFSALKHTICEIADK 595
Query: 541 ALTWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPAL 600
QGR +T H+ IWL+L+ SM +E E +K+ P+MD+YM N Y+SFALGPIVLPAL
Sbjct: 596 GFKLQGRSITDHIISIWLDLLYSMMKETELGIDKSFPTMDEYMSNAYVSFALGPIVLPAL 655
Query: 601 YFIGPKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNR 660
Y +GPKLSEE+V EYH LFKLMSTCGRLLND++ ERE +GK++A+SL+++N GG
Sbjct: 656 YLVGPKLSEEMVNHSEYHTLFKLMSTCGRLLNDIRGYERELKDGKISAVSLYIMNNGGEI 715
Query: 661 TNEEVIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSL 720
T E I + I++ RRELLRLVL+E S +P+ACK LFW M VVHLFYRKDDGFTSL
Sbjct: 716 TTEAAISEMRSWIERDRRELLRLVLEENKSVLPKACKKLFWHMCTVVHLFYRKDDGFTSL 775
Query: 721 GLMNSAKALFEEPI 730
L A+ EPI
Sbjct: 776 DLHGVVNAIINEPI 789
BLAST of Sgr028675 vs. ExPASy Swiss-Prot
Match:
A0A2K9RFY0 (Ent-kaurene synthase TSP4, chloroplastic OS=Vitex agnus-castus OX=54477 GN=TPS4 PE=1 SV=1)
HSP 1 Score: 822.8 bits (2124), Expect = 3.1e-237
Identity = 404/732 (55.19%), Postives = 532/732 (72.68%), Query Frame = 0
Query: 1 MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
+ K ELSVS YDTAW+A+VP+PN + P FP+C++WLL NQ DGSW H HP+L K
Sbjct: 55 LIHKAELSVSTYDTAWVAMVPSPNSSQEPCFPDCLSWLLQNQCCDGSWACPHHHPLLKKD 114
Query: 61 TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
L STLAC+L LK+W +G+E + +G++FIE N SA ++ Q SP+GFDIIFP M++YA+
Sbjct: 115 VLCSTLACVLALKKWGVGEEKINRGVHFIEHNFASAMEKCQISPMGFDIIFPAMLDYARD 174
Query: 121 LELNLPLTQMNVDALIHRRELEL--NRSNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRKN 180
L LNL L ++ LI++R LEL N+++S R+AYLAYV+EG+GK QD VM QR+N
Sbjct: 175 LLLNLRLEPTMLNDLIYKRGLELKRNQNHSAEREAYLAYVAEGMGKLQDLGSVMKHQRRN 234
Query: 181 GSLFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLGI 240
GSLFNSPSTTAAAF+ N CL YL S L+KFG+AVP +YPLD Y QLC VD+L+ LGI
Sbjct: 235 GSLFNSPSTTAAAFIAFPNSRCLTYLRSALKKFGSAVPAVYPLDIYLQLCTVDNLERLGI 294
Query: 241 DHHFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDRF 300
+F++EI VLD+TYR WLQG E I +DA TCA+AFR+LR GY V+SD + + E+ F
Sbjct: 295 SRYFQKEIQGVLDETYRCWLQGNEEIFMDAPTCALAFRVLRKNGYNVTSDPITKLLEECF 354
Query: 301 SDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRLHRN 360
S G +KDI + L L++AS+ +YP++ LE N + L LSS+ IHS +L RN
Sbjct: 355 SSSFCGNIKDINTTLGLYRASEFILYPDERDLEKQNLMLKNLLEQELSSDFIHSSQLGRN 414
Query: 361 MKQEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMEDFN 420
+ EV AL++ + L+R+ + IEHY+ +N RILKT Y N G++DFL L+++D+N
Sbjct: 415 IDAEVKHALEYPFYADLDRIVNRRNIEHYNFDNTRILKTSYCSPNFGNKDFLFLSVKDYN 474
Query: 421 VCQCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKNGV 480
CQ IHR+E ++LERWV+EN+LD+L ARQK YCYF+AAA + +PELS+AR+SWAKNGV
Sbjct: 475 ECQAIHREELRELERWVIENRLDELRFARQKCAYCYFSAAATLFAPELSNARMSWAKNGV 534
Query: 481 LTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKAL 540
LTTVVDD FD+ GS +E NL+Q VE WDV E S+ V+IIF AL T+ +IG+K
Sbjct: 535 LTTVVDDFFDLGGSVEELKNLIQLVELWDVDVSTECSSQNVQIIFSALKCTICDIGDKGS 594
Query: 541 TWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPALYF 600
Q R +T+H+ DIWL+L+ SM +E EW +K +P+MD+Y+ N Y+SFALGPIVLPALY
Sbjct: 595 KLQERSITNHIIDIWLDLLYSMMKETEWARDKYIPTMDEYISNAYVSFALGPIVLPALYL 654
Query: 601 IGPKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNRTN 660
+GPKLSEE+V EYHNL+KLMSTCGRLLND++ ERE EGKLNA+ L++IN GG T
Sbjct: 655 VGPKLSEEMVHHSEYHNLYKLMSTCGRLLNDIRGCERELKEGKLNAIPLYIINNGGEITK 714
Query: 661 EEVIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSLGL 720
E ++ I+ RRELLRLVL+ K+S +P++CK+LFW MS V+HLFY KDDGFTS L
Sbjct: 715 EAAASEMKSLIETHRRELLRLVLEGKNSVLPKSCKELFWHMSKVLHLFYSKDDGFTSQDL 774
Query: 721 MNSAKALFEEPI 730
+ KA+ EPI
Sbjct: 775 IKVVKAVIYEPI 786
BLAST of Sgr028675 vs. ExPASy Swiss-Prot
Match:
A0A0U4CDK4 (Ent-kaur-16-ene synthase, chloroplastic OS=Salvia miltiorrhiza OX=226208 GN=KS PE=1 SV=1)
HSP 1 Score: 820.5 bits (2118), Expect = 1.6e-236
Identity = 398/733 (54.30%), Postives = 522/733 (71.21%), Query Frame = 0
Query: 1 MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
+F K ELSVS YDTAW+A+VP+P L+ P FP+C+NWLL+NQ DGSW H HP+L K
Sbjct: 59 LFHKDELSVSTYDTAWVAMVPSPTSLEEPCFPDCLNWLLENQCHDGSWARPHHHPLLKKD 118
Query: 61 TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
L STLAC+L LK+W +G+E + +GL+F+E N SATD+ Q +P+GFDIIFP M++YA+
Sbjct: 119 VLSSTLACILALKKWGVGEEQIKRGLHFLELNFASATDKCQITPMGFDIIFPAMLDYARG 178
Query: 121 LELNLPLTQMNVDALIHRRELELNRSN---SEGRKAYLAYVSEGIGKSQDWDMVMNFQRK 180
LNL L + L+H+R++EL RSN S + Y AY++EG+G+ Q+W+ VM +QR+
Sbjct: 179 FSLNLRLDPTTFNDLMHKRDVELKRSNRNYSSETETYWAYIAEGMGELQNWESVMKYQRR 238
Query: 181 NGSLFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLG 240
NGSLFN PSTTAAAF+ L N CL+YLH L+KFGNAV +YPLD Y+QLC VD+L+ LG
Sbjct: 239 NGSLFNCPSTTAAAFIALRNSDCLNYLHLALKKFGNAVSAVYPLDIYSQLCTVDNLERLG 298
Query: 241 IDHHFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDR 300
I +F EI VLD+TYR W+QG E I +DASTCA+AFR LR+ GY+V+SD + Q ++
Sbjct: 299 ISQYFSTEIQNVLDETYRCWMQGNEEIFMDASTCALAFRTLRLNGYDVTSDPVTQILQEC 358
Query: 301 FSDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRLHR 360
FS G + DI + LEL++AS++ +YP++ LE N L LSS I S +L R
Sbjct: 359 FSSSFRGNMTDINTTLELYRASELVLYPDERDLEKQNLRLKLLLEQELSSGLIQSCQLGR 418
Query: 361 NMKQEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMEDF 420
++ EV A+++ + ++R+ + +IEHY+ +N RILKT Y N ++DFL L++EDF
Sbjct: 419 SINVEVNQAIEYPFYAIMDRVAKRKSIEHYNFDNTRILKTSYCSPNFSNEDFLFLSIEDF 478
Query: 421 NVCQCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKNG 480
N CQ HR+E +LERWVVEN+LD+L AR K YCYF+AAA +PEL DARLSWAKNG
Sbjct: 479 NRCQAAHREELGELERWVVENRLDELKFARSKSAYCYFSAAATFFAPELLDARLSWAKNG 538
Query: 481 VLTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKA 540
VLTTV+DD FDV GS +E NL+Q VE WDV E S V+IIF AL T+ EIG+KA
Sbjct: 539 VLTTVIDDFFDVGGSVEELKNLIQLVELWDVDISTECYSHNVQIIFSALRRTICEIGDKA 598
Query: 541 LTWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPALY 600
QGRC+T+H+ IWL+L+ SM +E EW + VP++D+YM N ++SFALGPIVLPALY
Sbjct: 599 FKLQGRCITNHIIAIWLDLLNSMMRETEWARDNFVPTIDEYMSNAHVSFALGPIVLPALY 658
Query: 601 FIGPKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNRT 660
+GPKLSEE+V EYHNLFKLMSTCGRLLND+ ERE +GKLNALSL++IN GG +
Sbjct: 659 LVGPKLSEEMVNHSEYHNLFKLMSTCGRLLNDIHGYERELKDGKLNALSLYIINHGGEVS 718
Query: 661 NEEVIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSLG 720
E I ++ I+ RRELLRLVL+ K S +P+ C++LFW M VVHLFY K DGFTS
Sbjct: 719 KEAAIWEMKSWIETQRRELLRLVLEGKKSVLPKPCRELFWHMCSVVHLFYSKGDGFTSQD 778
Query: 721 LMNSAKALFEEPI 730
L+ + +PI
Sbjct: 779 LIQLVNTIIHQPI 791
BLAST of Sgr028675 vs. ExPASy Swiss-Prot
Match:
A0A3G1QTS7 (Ent-kaurene synthase 1, chloroplastic OS=Isodon eriocalyx OX=662907 GN=KS1 PE=1 SV=1)
HSP 1 Score: 809.7 bits (2090), Expect = 2.8e-233
Identity = 398/734 (54.22%), Postives = 523/734 (71.25%), Query Frame = 0
Query: 1 MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
+F K ELSVS YDTAW+A+VP+P + P FP C+NWLL+NQ DGSW H H +L K
Sbjct: 56 LFHKNELSVSTYDTAWVAMVPSPTSSEEPCFPACLNWLLENQCHDGSWARPHHHHMLKKD 115
Query: 61 TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
L STLAC+L LK+W +G+E + +GL+F+E N SAT++ Q +P+GFDIIFP M++ A+
Sbjct: 116 VLSSTLACILALKKWGVGEEQINRGLHFVELNFASATEKGQITPMGFDIIFPAMLDNARG 175
Query: 121 LELNLPLTQMNVDALIHRRELEL---NRSNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRK 180
L LNL L ++ LI++R+LEL N+SNS ++ Y A+++EG+GK DW+ VM +QRK
Sbjct: 176 LSLNLQLEPTMLNDLIYKRDLELKRCNQSNSAEKEVYWAHIAEGMGKLHDWESVMKYQRK 235
Query: 181 NGSLFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLG 240
NGSLFN PSTTAAAF L N CL+YL L+KFG+AVP +YPLD Y+QLC V +L+ LG
Sbjct: 236 NGSLFNCPSTTAAAFTALRNSDCLNYLCLALEKFGSAVPAVYPLDIYSQLCTVGNLERLG 295
Query: 241 IDHHFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDR 300
I +F EI VLD+TYR WLQG+E I +DASTCA+AFR LR+ GY V+SD + + ++
Sbjct: 296 ISRYFLTEIQSVLDETYRSWLQGDEEIFMDASTCALAFRTLRMNGYNVTSDPITKILQEC 355
Query: 301 FSDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNS-IHSDRLH 360
FS G + D + LE+++AS++ +YPE+ L N FL LSSN I S +L
Sbjct: 356 FSSSFRGNMTDNNTTLEIYRASELILYPEERDLVQHNLRLKTFLEQELSSNGFIQSCQLG 415
Query: 361 RNMKQEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMED 420
RN+ EV A+++ + ++R+ + IE+Y+++N RILKT Y N G++DFL L++ED
Sbjct: 416 RNINAEVNQAIEYPFYAIMDRMAKRKNIENYNIDNTRILKTSYRSPNFGNKDFLSLSVED 475
Query: 421 FNVCQCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKN 480
FN CQ IHR+E ++LERWV+EN+LD+L AR K YCYF+AAA I SPELSDAR+SWAKN
Sbjct: 476 FNRCQVIHREELRELERWVIENRLDELKFARSKAAYCYFSAAATIFSPELSDARMSWAKN 535
Query: 481 GVLTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNK 540
GVLTTVVDD FDV GS +E NL+Q VE WDV + CS V+IIF AL T+ EI +K
Sbjct: 536 GVLTTVVDDFFDVGGSVEELKNLIQLVELWDVDVSTQCCSPNVQIIFSALKHTICEIADK 595
Query: 541 ALTWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPAL 600
QGR +T H+ IWL+L+ SM +E E +K+ P+MD+YM N Y+SFALGPIVLPAL
Sbjct: 596 GFKLQGRSITDHIISIWLDLLYSMMKETELGIDKSFPTMDEYMSNAYVSFALGPIVLPAL 655
Query: 601 YFIGPKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNR 660
Y +GPKLSEE+V EYH LFKLMSTCGRLLND++ ERE +GK++A+SL+++N GG
Sbjct: 656 YLVGPKLSEEMVNHSEYHTLFKLMSTCGRLLNDIRGYERELKDGKISAVSLYIMNNGGEI 715
Query: 661 TNEEVIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSL 720
T E I + I++ RRELLRLVL+E S +P+ACK LFW M VVH+FY KDDGFTSL
Sbjct: 716 TTEAAISEMRSWIERDRRELLRLVLEENKSVLPKACKKLFWHMCTVVHVFYSKDDGFTSL 775
Query: 721 GLMNSAKALFEEPI 730
L A+ EPI
Sbjct: 776 NLHGVVNAIINEPI 789
BLAST of Sgr028675 vs. ExPASy TrEMBL
Match:
A0A6J1D2F0 (ent-kaur-16-ene synthase, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016726 PE=4 SV=1)
HSP 1 Score: 1117.4 bits (2889), Expect = 0.0e+00
Identity = 539/735 (73.33%), Postives = 625/735 (85.03%), Query Frame = 0
Query: 1 MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
MF KVELSVSAYDTAW+A+VP+PN L PLFPECINW+LDNQ PDGSWG +H H +L KA
Sbjct: 96 MFNKVELSVSAYDTAWVAMVPSPNSLNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKA 155
Query: 61 TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
+LLSTLAC L LKRW+IG EHM K LNFI+SN+ SATDEKQ SP+GFDIIFPGMIEYAK
Sbjct: 156 SLLSTLACALTLKRWNIGHEHMSKALNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKD 215
Query: 121 LELNLPLTQMNVDALIHRRELELNRSNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRKNGS 180
L+LNLPL NVDAL+ ++EL+L RSNSEG KAYLAYVSEGIG+ +DW+MVM +QRKNGS
Sbjct: 216 LDLNLPLAPANVDALLQKKELDLRRSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKNGS 275
Query: 181 LFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLGIDH 240
LFNSPSTTA AFM+ N+D C +YLHSLLQKF N+VPTIYPLD YA+LCMVD+LQ LGID
Sbjct: 276 LFNSPSTTAVAFMHGNDDGCFNYLHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGIDR 335
Query: 241 HFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDRFSD 300
HFKEEI VLD+TYR W+QGEE+I LDASTCAMAFRMLR+ GY+VSSDQL QFSED FS+
Sbjct: 336 HFKEEIRSVLDETYRCWMQGEEDIFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLFSN 395
Query: 301 CLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRLHRNMK 360
CLGGYLKD GS LELFKASQI IYP++ +LENINSWT+ FL++GLSS S+HSDR R +K
Sbjct: 396 CLGGYLKDFGSSLELFKASQIIIYPDESILENINSWTNSFLSHGLSSGSVHSDRPDRLLK 455
Query: 361 QEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMEDFNVC 420
QE I+AL+FTY+ TLERL K A+E+Y+++ +RI KT Y+CLN GHQDFL LA+EDFN+
Sbjct: 456 QEAINALEFTYSNTLERLLNKRAMENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFNIL 515
Query: 421 QCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKNGVLT 480
Q IH +E K+LERWVVEN+LDKL ARQKL YCYF+AAA +TSPEL DARLSWAKNGVLT
Sbjct: 516 QSIHHKELKELERWVVENRLDKLKFARQKLAYCYFSAAATLTSPELRDARLSWAKNGVLT 575
Query: 481 TVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKALTW 540
TVVDD FDV GSE E INL+Q VE+WDV + +YCS++VEIIF ALH TV EI +AL W
Sbjct: 576 TVVDDFFDVGGSEGELINLIQLVEKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRALPW 635
Query: 541 QGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPALYFIG 600
QGR V +V DIWL L++SMR+EAEWL NK P MD+YMENGYISFALGPIVLPAL+F+G
Sbjct: 636 QGRSVMRNVIDIWLALLKSMRKEAEWLKNKIAPPMDEYMENGYISFALGPIVLPALFFVG 695
Query: 601 PKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNRTNEE 660
PKL EEV+GS EYH LFKLMSTCGRLLND Q+ ERESSEGKLNA+SL MIN GG+ T EE
Sbjct: 696 PKLPEEVIGSCEYHKLFKLMSTCGRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTKEE 755
Query: 661 VIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSLGLMN 720
AI+ +++++RRELLRLVLQEK+ST+PRACKDLFW+MSCVVHLFYRKDDGFTS LMN
Sbjct: 756 ASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHELMN 815
Query: 721 SAKALFEEPISFDNL 735
SAKAL E+P+ D L
Sbjct: 816 SAKALLEQPMVLDEL 830
BLAST of Sgr028675 vs. ExPASy TrEMBL
Match:
A0A6J1D2L1 (ent-kaur-16-ene synthase, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016726 PE=4 SV=1)
HSP 1 Score: 1112.4 bits (2876), Expect = 0.0e+00
Identity = 539/737 (73.13%), Postives = 625/737 (84.80%), Query Frame = 0
Query: 1 MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
MF KVELSVSAYDTAW+A+VP+PN L PLFPECINW+LDNQ PDGSWG +H H +L KA
Sbjct: 96 MFNKVELSVSAYDTAWVAMVPSPNSLNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKA 155
Query: 61 TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
+LLSTLAC L LKRW+IG EHM K LNFI+SN+ SATDEKQ SP+GFDIIFPGMIEYAK
Sbjct: 156 SLLSTLACALTLKRWNIGHEHMSKALNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKD 215
Query: 121 LELNLPLTQMNVDALIHRRELELN--RSNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRKN 180
L+LNLPL NVDAL+ ++EL+L RSNSEG KAYLAYVSEGIG+ +DW+MVM +QRKN
Sbjct: 216 LDLNLPLAPANVDALLQKKELDLRSCRSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKN 275
Query: 181 GSLFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLGI 240
GSLFNSPSTTA AFM+ N+D C +YLHSLLQKF N+VPTIYPLD YA+LCMVD+LQ LGI
Sbjct: 276 GSLFNSPSTTAVAFMHGNDDGCFNYLHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGI 335
Query: 241 DHHFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDRF 300
D HFKEEI VLD+TYR W+QGEE+I LDASTCAMAFRMLR+ GY+VSSDQL QFSED F
Sbjct: 336 DRHFKEEIRSVLDETYRCWMQGEEDIFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLF 395
Query: 301 SDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRLHRN 360
S+CLGGYLKD GS LELFKASQI IYP++ +LENINSWT+ FL++GLSS S+HSDR R
Sbjct: 396 SNCLGGYLKDFGSSLELFKASQIIIYPDESILENINSWTNSFLSHGLSSGSVHSDRPDRL 455
Query: 361 MKQEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMEDFN 420
+KQE I+AL+FTY+ TLERL K A+E+Y+++ +RI KT Y+CLN GHQDFL LA+EDFN
Sbjct: 456 LKQEAINALEFTYSNTLERLLNKRAMENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFN 515
Query: 421 VCQCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKNGV 480
+ Q IH +E K+LERWVVEN+LDKL ARQKL YCYF+AAA +TSPEL DARLSWAKNGV
Sbjct: 516 ILQSIHHKELKELERWVVENRLDKLKFARQKLAYCYFSAAATLTSPELRDARLSWAKNGV 575
Query: 481 LTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKAL 540
LTTVVDD FDV GSE E INL+Q VE+WDV + +YCS++VEIIF ALH TV EI +AL
Sbjct: 576 LTTVVDDFFDVGGSEGELINLIQLVEKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRAL 635
Query: 541 TWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPALYF 600
WQGR V +V DIWL L++SMR+EAEWL NK P MD+YMENGYISFALGPIVLPAL+F
Sbjct: 636 PWQGRSVMRNVIDIWLALLKSMRKEAEWLKNKIAPPMDEYMENGYISFALGPIVLPALFF 695
Query: 601 IGPKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNRTN 660
+GPKL EEV+GS EYH LFKLMSTCGRLLND Q+ ERESSEGKLNA+SL MIN GG+ T
Sbjct: 696 VGPKLPEEVIGSCEYHKLFKLMSTCGRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTK 755
Query: 661 EEVIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSLGL 720
EE AI+ +++++RRELLRLVLQEK+ST+PRACKDLFW+MSCVVHLFYRKDDGFTS L
Sbjct: 756 EEASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHEL 815
Query: 721 MNSAKALFEEPISFDNL 735
MNSAKAL E+P+ D L
Sbjct: 816 MNSAKALLEQPMVLDEL 832
BLAST of Sgr028675 vs. ExPASy TrEMBL
Match:
A0A6J1D1G1 (ent-kaur-16-ene synthase, chloroplastic isoform X3 OS=Momordica charantia OX=3673 GN=LOC111016726 PE=4 SV=1)
HSP 1 Score: 1112.4 bits (2876), Expect = 0.0e+00
Identity = 539/737 (73.13%), Postives = 625/737 (84.80%), Query Frame = 0
Query: 1 MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
MF KVELSVSAYDTAW+A+VP+PN L PLFPECINW+LDNQ PDGSWG +H H +L KA
Sbjct: 83 MFNKVELSVSAYDTAWVAMVPSPNSLNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKA 142
Query: 61 TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
+LLSTLAC L LKRW+IG EHM K LNFI+SN+ SATDEKQ SP+GFDIIFPGMIEYAK
Sbjct: 143 SLLSTLACALTLKRWNIGHEHMSKALNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKD 202
Query: 121 LELNLPLTQMNVDALIHRRELELN--RSNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRKN 180
L+LNLPL NVDAL+ ++EL+L RSNSEG KAYLAYVSEGIG+ +DW+MVM +QRKN
Sbjct: 203 LDLNLPLAPANVDALLQKKELDLRSCRSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKN 262
Query: 181 GSLFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLGI 240
GSLFNSPSTTA AFM+ N+D C +YLHSLLQKF N+VPTIYPLD YA+LCMVD+LQ LGI
Sbjct: 263 GSLFNSPSTTAVAFMHGNDDGCFNYLHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGI 322
Query: 241 DHHFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDRF 300
D HFKEEI VLD+TYR W+QGEE+I LDASTCAMAFRMLR+ GY+VSSDQL QFSED F
Sbjct: 323 DRHFKEEIRSVLDETYRCWMQGEEDIFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLF 382
Query: 301 SDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRLHRN 360
S+CLGGYLKD GS LELFKASQI IYP++ +LENINSWT+ FL++GLSS S+HSDR R
Sbjct: 383 SNCLGGYLKDFGSSLELFKASQIIIYPDESILENINSWTNSFLSHGLSSGSVHSDRPDRL 442
Query: 361 MKQEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMEDFN 420
+KQE I+AL+FTY+ TLERL K A+E+Y+++ +RI KT Y+CLN GHQDFL LA+EDFN
Sbjct: 443 LKQEAINALEFTYSNTLERLLNKRAMENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFN 502
Query: 421 VCQCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKNGV 480
+ Q IH +E K+LERWVVEN+LDKL ARQKL YCYF+AAA +TSPEL DARLSWAKNGV
Sbjct: 503 ILQSIHHKELKELERWVVENRLDKLKFARQKLAYCYFSAAATLTSPELRDARLSWAKNGV 562
Query: 481 LTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKAL 540
LTTVVDD FDV GSE E INL+Q VE+WDV + +YCS++VEIIF ALH TV EI +AL
Sbjct: 563 LTTVVDDFFDVGGSEGELINLIQLVEKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRAL 622
Query: 541 TWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPALYF 600
WQGR V +V DIWL L++SMR+EAEWL NK P MD+YMENGYISFALGPIVLPAL+F
Sbjct: 623 PWQGRSVMRNVIDIWLALLKSMRKEAEWLKNKIAPPMDEYMENGYISFALGPIVLPALFF 682
Query: 601 IGPKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNRTN 660
+GPKL EEV+GS EYH LFKLMSTCGRLLND Q+ ERESSEGKLNA+SL MIN GG+ T
Sbjct: 683 VGPKLPEEVIGSCEYHKLFKLMSTCGRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTK 742
Query: 661 EEVIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSLGL 720
EE AI+ +++++RRELLRLVLQEK+ST+PRACKDLFW+MSCVVHLFYRKDDGFTS L
Sbjct: 743 EEASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHEL 802
Query: 721 MNSAKALFEEPISFDNL 735
MNSAKAL E+P+ D L
Sbjct: 803 MNSAKALLEQPMVLDEL 819
BLAST of Sgr028675 vs. ExPASy TrEMBL
Match:
A0A1S4DWJ8 (ent-kaur-16-ene synthase, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489622 PE=4 SV=1)
HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 530/735 (72.11%), Postives = 618/735 (84.08%), Query Frame = 0
Query: 1 MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
+F+ VELSVSAYDTAW+A+VP+PN L PLFPECINW+LD+Q PDGSWG LH H ++ KA
Sbjct: 47 LFKNVELSVSAYDTAWVAMVPSPNSLNKPLFPECINWVLDHQNPDGSWGILHDHQLVMKA 106
Query: 61 TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
TLLSTLAC+L LKRW+IG +HM K L+FI+SN+ SATDE QRSP+GFDIIFPGMIEYAK
Sbjct: 107 TLLSTLACVLTLKRWNIGHDHMSKALSFIKSNIASATDENQRSPVGFDIIFPGMIEYAKD 166
Query: 121 LELNLPLTQMNVDALIHRRELELNRSNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRKNGS 180
L LNLPL MNVDAL+ ++ELEL RSNSEG KAYLAYVSEGIGK QDWDMVM +QRKNGS
Sbjct: 167 LNLNLPLASMNVDALVQKKELEL-RSNSEGGKAYLAYVSEGIGKLQDWDMVMQYQRKNGS 226
Query: 181 LFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLGIDH 240
LF+SPSTTA AFM+ N+D C +YL S+LQKF ++VP IYPLD YA+L MVDSLQ LGID
Sbjct: 227 LFSSPSTTAVAFMHRNDDGCFNYLRSILQKFHSSVPAIYPLDIYARLHMVDSLQKLGIDR 286
Query: 241 HFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDRFSD 300
HFK+EI VLD+TYR W+QGEENI LDASTCAMAFRMLRV GY+VSSDQL QFSED F +
Sbjct: 287 HFKDEIRSVLDETYRCWMQGEENIFLDASTCAMAFRMLRVEGYDVSSDQLTQFSEDLFPN 346
Query: 301 CLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRLHRNMK 360
CLGG+LKD G+ LELFKASQI IYP++ +LENINSWTS FLN+GLS S+HSDR R +K
Sbjct: 347 CLGGHLKDFGASLELFKASQIIIYPDEFILENINSWTSRFLNHGLSGGSVHSDRTERLVK 406
Query: 361 QEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMEDFNVC 420
QE ++A +F YN+TLERL K A+E YS + +RI KT Y+CLN GHQDFL LA+EDFN
Sbjct: 407 QEAVNAFEFPYNSTLERLSNKRALESYSGDIVRISKTTYACLNFGHQDFLELAVEDFNTL 466
Query: 421 QCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKNGVLT 480
QCIHR+E K+LE+WV+ENKLDKL ARQKL YCYF+AAA +TSPEL DARLSWAKNGVLT
Sbjct: 467 QCIHRKELKELEKWVIENKLDKLKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGVLT 526
Query: 481 TVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKALTW 540
TVVDD FDV GSE+E +NL+Q VE+WD + YCS++VEIIF ALHST+ EIG KAL W
Sbjct: 527 TVVDDFFDVGGSEEELVNLIQLVEKWDASGEAGYCSKEVEIIFLALHSTICEIGEKALPW 586
Query: 541 QGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPALYFIG 600
QGR VT +V DIWL L+ SMR+EAEWL NK VPS+D+YMENGY+SFALGPIVLP LYF+G
Sbjct: 587 QGRSVTRNVIDIWLALLESMRKEAEWLKNKVVPSLDEYMENGYVSFALGPIVLPTLYFVG 646
Query: 601 PKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNRTNEE 660
PKL EE++G+ EY LFKLMST GRLLND +T +RESSEGKLNALSL++I+ GG + EE
Sbjct: 647 PKLPEEIIGNCEYQKLFKLMSTSGRLLNDTRTFDRESSEGKLNALSLYLISAGGKLSKEE 706
Query: 661 VIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSLGLMN 720
A++ D+D++RRELLRLVLQE +STIPRACKDLFW+MSCVVHLFYRKDDGFTS LMN
Sbjct: 707 ATEAMKGDVDRTRRELLRLVLQE-NSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHELMN 766
Query: 721 SAKALFEEPISFDNL 735
SAKALFE P+ D L
Sbjct: 767 SAKALFELPMVLDEL 779
BLAST of Sgr028675 vs. ExPASy TrEMBL
Match:
A0A1S3BG06 (ent-kaur-16-ene synthase, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489622 PE=4 SV=1)
HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 529/737 (71.78%), Postives = 617/737 (83.72%), Query Frame = 0
Query: 1 MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
+F+ VELSVSAYDTAW+A+VP+PN L PLFPECINW+LD+Q PDGSWG LH H ++ KA
Sbjct: 47 LFKNVELSVSAYDTAWVAMVPSPNSLNKPLFPECINWVLDHQNPDGSWGILHDHQLVMKA 106
Query: 61 TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
TLLSTLAC+L LKRW+IG +HM K L+FI+SN+ SATDE QRSP+GFDIIFPGMIEYAK
Sbjct: 107 TLLSTLACVLTLKRWNIGHDHMSKALSFIKSNIASATDENQRSPVGFDIIFPGMIEYAKD 166
Query: 121 LELNLPLTQMNVDALIHRRELELNR--SNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRKN 180
L LNLPL MNVDAL+ ++ELEL SNSEG KAYLAYVSEGIGK QDWDMVM +QRKN
Sbjct: 167 LNLNLPLASMNVDALVQKKELELRSCCSNSEGGKAYLAYVSEGIGKLQDWDMVMQYQRKN 226
Query: 181 GSLFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLGI 240
GSLF+SPSTTA AFM+ N+D C +YL S+LQKF ++VP IYPLD YA+L MVDSLQ LGI
Sbjct: 227 GSLFSSPSTTAVAFMHRNDDGCFNYLRSILQKFHSSVPAIYPLDIYARLHMVDSLQKLGI 286
Query: 241 DHHFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDRF 300
D HFK+EI VLD+TYR W+QGEENI LDASTCAMAFRMLRV GY+VSSDQL QFSED F
Sbjct: 287 DRHFKDEIRSVLDETYRCWMQGEENIFLDASTCAMAFRMLRVEGYDVSSDQLTQFSEDLF 346
Query: 301 SDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRLHRN 360
+CLGG+LKD G+ LELFKASQI IYP++ +LENINSWTS FLN+GLS S+HSDR R
Sbjct: 347 PNCLGGHLKDFGASLELFKASQIIIYPDEFILENINSWTSRFLNHGLSGGSVHSDRTERL 406
Query: 361 MKQEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMEDFN 420
+KQE ++A +F YN+TLERL K A+E YS + +RI KT Y+CLN GHQDFL LA+EDFN
Sbjct: 407 VKQEAVNAFEFPYNSTLERLSNKRALESYSGDIVRISKTTYACLNFGHQDFLELAVEDFN 466
Query: 421 VCQCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKNGV 480
QCIHR+E K+LE+WV+ENKLDKL ARQKL YCYF+AAA +TSPEL DARLSWAKNGV
Sbjct: 467 TLQCIHRKELKELEKWVIENKLDKLKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGV 526
Query: 481 LTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKAL 540
LTTVVDD FDV GSE+E +NL+Q VE+WD + YCS++VEIIF ALHST+ EIG KAL
Sbjct: 527 LTTVVDDFFDVGGSEEELVNLIQLVEKWDASGEAGYCSKEVEIIFLALHSTICEIGEKAL 586
Query: 541 TWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPALYF 600
WQGR VT +V DIWL L+ SMR+EAEWL NK VPS+D+YMENGY+SFALGPIVLP LYF
Sbjct: 587 PWQGRSVTRNVIDIWLALLESMRKEAEWLKNKVVPSLDEYMENGYVSFALGPIVLPTLYF 646
Query: 601 IGPKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNRTN 660
+GPKL EE++G+ EY LFKLMST GRLLND +T +RESSEGKLNALSL++I+ GG +
Sbjct: 647 VGPKLPEEIIGNCEYQKLFKLMSTSGRLLNDTRTFDRESSEGKLNALSLYLISAGGKLSK 706
Query: 661 EEVIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSLGL 720
EE A++ D+D++RRELLRLVLQE +STIPRACKDLFW+MSCVVHLFYRKDDGFTS L
Sbjct: 707 EEATEAMKGDVDRTRRELLRLVLQE-NSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHEL 766
Query: 721 MNSAKALFEEPISFDNL 735
MNSAKALFE P+ D L
Sbjct: 767 MNSAKALFELPMVLDEL 782
BLAST of Sgr028675 vs. TAIR 10
Match:
AT1G79460.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )
HSP 1 Score: 790.4 bits (2040), Expect = 1.2e-228
Identity = 404/737 (54.82%), Postives = 526/737 (71.37%), Query Frame = 0
Query: 1 MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGF-LHPHPILTK 60
M EKVELSVSAYDT+W+A+VP+P+ PLFP+C+ WLLDNQ DGSWG H H L K
Sbjct: 45 MLEKVELSVSAYDTSWVAMVPSPSSQNAPLFPQCVKWLLDNQHEDGSWGLDNHDHQSLKK 104
Query: 61 ATLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAK 120
L STLA +L LK+W IG+ + KGL FIE N TDE + P GFDIIFPGMI+YA+
Sbjct: 105 DVLSSTLASILALKKWGIGERQINKGLQFIELNSALVTDETIQKPTGFDIIFPGMIKYAR 164
Query: 121 TLELNLPLTQMNVDALIHRRELEL---NRSNSEGRKAYLAYVSEGIGKSQDWDMVMNFQR 180
L L +PL VD +I +R+L+L + S+GR+AYLAYV EG +DWD+++ +QR
Sbjct: 165 DLNLTIPLGSEVVDDMIRKRDLDLKCDSEKFSKGREAYLAYVLEGTRNLKDWDLIVKYQR 224
Query: 181 KNGSLFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTL 240
KNGSLF+SP+TTAAAF ND CL YL SLLQKF AVP++YP D YA+L ++ +L++L
Sbjct: 225 KNGSLFDSPATTAAAFTQFGNDGCLRYLCSLLQKFEAAVPSVYPFDQYARLSIIVTLESL 284
Query: 241 GIDHHFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSED 300
GID FK EI +LD+TYRYWL+G+E I LD +TCA+AFR+L +GY+VS D L F+E+
Sbjct: 285 GIDRDFKTEIKSILDETYRYWLRGDEEICLDLATCALAFRLLLAHGYDVSYDPLKPFAEE 344
Query: 301 R-FSDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRL 360
FSD L GY+K+ SVLELFKA+Q YP + L+ WT +L LSS + +
Sbjct: 345 SGFSDTLEGYVKNTFSVLELFKAAQ--SYPHESALKKQCCWTKQYLEMELSS-WVKTSVR 404
Query: 361 HRNMKQEVISALDFTYNTTLERLQYKSAIEHYS-VNNIRILKTPYSCLNLGHQDFLRLAM 420
+ +K+EV AL F +LER ++ I + S V N R+ KT Y N+ D L+LA+
Sbjct: 405 DKYLKKEVEDALAFPSYASLERSDHRRKILNGSAVENTRVTKTSYRLHNICTSDILKLAV 464
Query: 421 EDFNVCQCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWA 480
+DFN CQ IHR+E + L+RW+VEN+L +L ARQKL YCYF+ AA + SPELSDAR+SWA
Sbjct: 465 DDFNFCQSIHREEMERLDRWIVENRLQELKFARQKLAYCYFSGAATLFSPELSDARISWA 524
Query: 481 KNGVLTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIG 540
K GVLTTVVDD FDV GS++E NL+ VE+WD+ EY SE VEIIF L T+ E G
Sbjct: 525 KGGVLTTVVDDFFDVGGSKEELENLIHLVEKWDLNGVPEYSSEHVEIIFSVLRDTILETG 584
Query: 541 NKALTWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLP 600
+KA T+QGR VT H+ IWL+L++SM +EAEW S+K+ PS++DYMEN YISFALGPIVLP
Sbjct: 585 DKAFTYQGRNVTHHIVKIWLDLLKSMLREAEWSSDKSTPSLEDYMENAYISFALGPIVLP 644
Query: 601 ALYFIGPKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGG 660
A Y IGP L E+ V S +Y+ L+KL+ST GRLLND+Q +RES+EGKLNA+SLHM +
Sbjct: 645 ATYLIGPPLPEKTVDSHQYNQLYKLVSTMGRLLNDIQGFKRESAEGKLNAVSLHMKHERD 704
Query: 661 NRTNEEVIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFT 720
NR+ E +I +++ ++ R EL +LVL+EK S +PR CK+ F +MS V++LFYRKDDGFT
Sbjct: 705 NRSKEVIIESMKGLAERKREELHKLVLEEKGSVVPRECKEAFLKMSKVLNLFYRKDDGFT 764
Query: 721 SLGLMNSAKALFEEPIS 731
S LM+ K++ EP+S
Sbjct: 765 SNDLMSLVKSVIYEPVS 778
BLAST of Sgr028675 vs. TAIR 10
Match:
AT1G61120.1 (terpene synthase 04 )
HSP 1 Score: 288.9 bits (738), Expect = 1.2e-77
Identity = 224/769 (29.13%), Postives = 370/769 (48.11%), Query Frame = 0
Query: 7 LSVSAYDTAWMALVP-----TPNCLKTPLFPECINWLLDNQ-LPDGSWGFLHPHPILT-- 66
+S SAYDTAW++++ N LK P+F C++W++ NQ +G W + +
Sbjct: 36 VSPSAYDTAWLSMIEEDINVDDNELK-PMFQGCLDWIMCNQNAREGFWMNSTSYTTVADG 95
Query: 67 --------KATLLSTLACLLPLKRWDIGQEHMIKGLNFIESN----LDSATDEKQRSPIG 126
L STLAC++ L++W+IG H+ KG +IE N + +E+ P
Sbjct: 96 RDEDGEKDMCILTSTLACVVALQKWNIGCFHLHKGTRYIERNTEMIIGKYINEEGSYPRW 155
Query: 127 FDIIFPGMIEYAKTLELNLPLTQMNVDAL---------IHRRELELNRSNSEGRKAYLAY 186
F I F G++E A+ L L+ + ++ + I +RE ++ N + AYL
Sbjct: 156 FAIKFTGILELAQKLGLHFVFSSRCIEMIKGMFYQRQEIIQREKLVHDCNYKPLLAYLEV 215
Query: 187 VSEGIGKSQDWDMVM-NFQRKNGSLFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVP 246
+ + + D+++ + +GSLF SPS TA+AFM N CL YL +L+QK N VP
Sbjct: 216 LPSKLYVTNQEDIIVKSLDSMDGSLFQSPSATASAFMLTRNTKCLAYLQNLVQKCPNGVP 275
Query: 247 TIYPL-DTYAQLCMVDSLQTLGIDHHFKEEIGRVLDKTY-RYWLQGEENILL-----DAS 306
YPL + +L MV+ +++ G+ F EI VL++ Y RY + E + +
Sbjct: 276 QKYPLNEDLIKLSMVNLIESTGLGEFFGIEIEHVLEQVYSRYEEKDFERMPMSYLADQLH 335
Query: 307 TCAMAFRMLRVYGYEVSSDQLAQFSEDRFSDCLGGYLKDIGS----VLELFKASQITIYP 366
++AFRMLR++G +VS F D+ + ++I S +L +++A+ + ++P
Sbjct: 336 KDSLAFRMLRMHGRDVSPRSFCWFLNDQ--ETRNHLERNIDSFLLVILSVYRATDL-MFP 395
Query: 367 EDPVLENINSWTSHFLNYGLSSNSIHSDRLHRNMKQEVI-SALDFTYNTTLERLQYKSAI 426
+ L+ +T + L R++K+++I L + L+ L ++ I
Sbjct: 396 GEHDLQEAREYTRNLL------------EKRRSIKEKMIMHELSTPWIARLKHLDHRMWI 455
Query: 427 EHYSVNNIRILKTPYSCLNLGHQDFL-RLAMEDFNVCQCIHRQEKKDLERWVVENKLDKL 486
E + N + + K + L+ + D L LA +F Q + +E ++L WV + L +
Sbjct: 456 EDKNSNVLSMEKASFLRLHSSYSDKLTHLAARNFEFQQAKYCRELEELTMWVKKWGLSDI 515
Query: 487 -IARQKLVYCYFTAAAAITSPELSDARLSWAKNGVLTTVVDDLFDVWGSEDERINLVQAV 546
R+K YCYF ++ AK +L T+ DD FD GS ++ L +AV
Sbjct: 516 GFGREKTTYCYFATVTSLPYEYAIKFGKLAAKTAILITIADDFFDEKGSFNDLEGLTKAV 575
Query: 547 ERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKALTWQGRCVTSHVADIWLELIRSMRQE 606
RW + E IIF+AL V E N T + H+ +IW E S +E
Sbjct: 576 LRW----EGEELKSYGNIIFRALDDIVRETANTCRTHHKTDIIVHLRNIWGETFESWLRE 635
Query: 607 AEWLSNKAVPSMDDYMENGYISFALGPIVLPALYFIGPKLSEEVVGSFEYHNLFKLMSTC 666
AEW SMD+Y+ NG IS A I L + P + Y ++ L+
Sbjct: 636 AEWSKKGHTSSMDEYIRNGMISIAAHTIALSISCLMEPCFPHNKLKPGNYDSITTLLMII 695
Query: 667 GRLLNDVQTIERESSEGKLNALSLHMINTGGNRTNEEVIRAIEVDIDKSRRELLRLVLQE 726
RLLND+Q+ ++E +GK+N++ LHM N G E+ I IE ID R+E L VL +
Sbjct: 696 PRLLNDLQSYQKEQEQGKMNSVLLHMKNHPGLEI-EDSIAHIEKIIDSKRKEFLEHVLVD 755
Query: 727 KDSTIPRACKDLFWRMSCVVHLFYRKDDGFTS-LGLMNSAKALFEEPIS 731
S +P+ CK++ V +F+ K + + S +++ K +PI+
Sbjct: 756 GLSDLPKPCKEIHMSCCKVFEMFFNKKNRYDSNTEMLHDIKKALYDPIN 783
BLAST of Sgr028675 vs. TAIR 10
Match:
AT4G02780.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )
HSP 1 Score: 283.5 bits (724), Expect = 4.9e-76
Identity = 160/494 (32.39%), Postives = 272/494 (55.06%), Query Frame = 0
Query: 6 ELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKATLLST 65
E+++SAYDTAW+AL+ + KTP FP + W+ +NQL DGSWG + + L++T
Sbjct: 112 EITISAYDTAWVALIDAGD--KTPAFPSAVKWIAENQLSDGSWGDAYLFSYHDR--LINT 171
Query: 66 LACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEK-QRSPIGFDIIFPGMIEYAKTLELN 125
LAC++ L+ W++ KG+ F N+ DE + PIGF++ FP ++E A+ + ++
Sbjct: 172 LACVVALRSWNLFPHQCNKGITFFRENIGKLEDENDEHMPIGFEVAFPSLLEIARGINID 231
Query: 126 LPLTQMNVDALIHRRELELNRSNSE---GRKAYLAYVSEGIGKSQDWDMVMNFQRKNGSL 185
+P + + ++EL+L R E L + EG+ + DW+ ++ Q ++GS
Sbjct: 232 VPYDSPVLKDIYAKKELKLTRIPKEIMHKIPTTLLHSLEGM-RDLDWEKLLKLQSQDGSF 291
Query: 186 FNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLGIDHH 245
SPS+TA AFM + +CL+YL + +++F VP ++P+D + + +VD LQ LGI +
Sbjct: 292 LFSPSSTAFAFMQTRDSNCLEYLRNAVKRFNGGVPNVFPVDLFEHIWIVDRLQRLGISRY 351
Query: 246 FKEEIGRVLDKTYRYWLQ-----GEENILLDASTCAMAFRMLRVYGYEVSSDQLAQF-SE 305
F+EEI LD +RYW + + D AMAFR+LR +GY+VS+D F E
Sbjct: 352 FEEEIKECLDYVHRYWTDNGICWARCSHVQDIDDTAMAFRLLRQHGYQVSADVFKNFEKE 411
Query: 306 DRFSDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWT-SHFLNYGLSSNSIHSDR 365
F +G + + + L++ASQ+ +P + +L+N ++ ++ L I
Sbjct: 412 GEFFCFVGQSNQAVTGMFNLYRASQLA-FPREEILKNAKEFSYNYLLEKREREELIDKWI 471
Query: 366 LHRNMKQEVISALDFTYNTTLERLQYKSAIEHY-SVNNIRILKTPYSCLNLGHQDFLRLA 425
+ +++ E+ AL+ + +L R++ + I+ Y N++ I KT Y + + +L LA
Sbjct: 472 IMKDLPGEIGFALEIPWYASLPRVETRFYIDQYGGENDVWIGKTLYRMPYVNNNGYLELA 531
Query: 426 MEDFNVCQCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSW 485
+D+N CQ H+ E ++W EN+L + + R +L+ CY+ AAA I E S R+ W
Sbjct: 532 KQDYNNCQAQHQLEWDIFQKWYEENRLSEWGVRRSELLECYYLAAATIFESERSHERMVW 591
Query: 486 AKNGVLTTVVDDLF 487
AK+ VL + F
Sbjct: 592 AKSSVLVKAISSSF 599
BLAST of Sgr028675 vs. TAIR 10
Match:
AT4G16740.1 (terpene synthase 03 )
HSP 1 Score: 164.5 bits (415), Expect = 3.3e-40
Identity = 120/440 (27.27%), Postives = 220/440 (50.00%), Query Frame = 0
Query: 226 QLCMVDSLQTLGIDHHFKEEIGRVLDKTYRYWLQGEENIL-----LDASTCAMAFRMLRV 285
QL ++D+LQ LG+ +HF++EI + L + ++ +N + D A+ FR+LR
Sbjct: 77 QLELIDTLQRLGVSYHFEQEIKKTLTNVHVKNVRAHKNRIDRNRWGDLYATALEFRLLRQ 136
Query: 286 YGYEVSSDQLAQFSEDRFSDCLGGYL--KDIGSVLELFKASQITIYPEDPVLENINSWTS 345
+G+ + ++D F +G L KDI +L L++AS ++ + + E+I T
Sbjct: 137 HGFSI--------AQDVFDGNIGVDLDDKDIKGILSLYEASYLSTRIDTKLKESIYYTTK 196
Query: 346 HFLNY-GLSSNSIHSDRLHRNMKQEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKT 405
+ ++ N S L R VI AL+ Y+ + RL+ + IE Y +
Sbjct: 197 RLRKFVEVNKNETKSYTLRR----MVIHALEMPYHRRVGRLEARWYIEVYGERHDM---- 256
Query: 406 PYSCLNLGHQDFLRLAMEDFNVCQCIHRQEKKDLERWVVENKLDKLI--ARQKLVYCYFT 465
+ L LA DFN Q IH+ E K L W + L K + R ++ YF+
Sbjct: 257 --------NPILLELAKLDFNFVQAIHQDELKSLSSWWSKTGLTKHLDFVRDRITEGYFS 316
Query: 466 AAAAITSPELSDARLSWAKNGVLTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCS 525
+ + PE + R K +L T +DD++D++G+ +E VE+WDV N +E
Sbjct: 317 SVGVMYEPEFAYHRQMLTKVFMLITTIDDIYDIYGTLEELQLFTTIVEKWDV-NRLEELP 376
Query: 526 EKVEIIFKALHSTVDEIGNKALTWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMD 585
+++ F L + +++IG L +G V ++ + W ++ + +EA+W + P+ +
Sbjct: 377 NYMKLCFLCLVNEINQIGYFVLRDKGFNVIPYLKESWADMCTTFLKEAKWYKSGYKPNFE 436
Query: 586 DYMENGYISFALGPIVLPALYFIGPKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERE 645
+YM+NG+IS ++ P +L L+ + + +++GS+ H++ + +T RL ND+ T E
Sbjct: 437 EYMQNGWISSSV-PTILLHLFCLLSDQTLDILGSYN-HSVVRSSATILRLANDLATSSEE 489
Query: 646 SSEG-KLNALSLHMINTGGN 655
+ G + ++ HM TG +
Sbjct: 497 LARGDTMKSVQCHMHETGAS 489
BLAST of Sgr028675 vs. TAIR 10
Match:
AT2G24210.1 (terpene synthase 10 )
HSP 1 Score: 154.5 bits (389), Expect = 3.4e-37
Identity = 114/435 (26.21%), Postives = 206/435 (47.36%), Query Frame = 0
Query: 226 QLCMVDSLQTLGIDHHFKEEIGRVLDKTYRYWLQGEENIL-LDASTCAMAFRMLRVYGYE 285
QL +D LQ LG+ +HF+ EI +L +Y+ + NI D A+ FR+ R +G+
Sbjct: 98 QLEFIDDLQKLGVSYHFEAEIDNILTSSYK---KDRTNIQESDLHATALEFRLFRQHGFN 157
Query: 286 VSSDQLAQFSEDRFSDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYG 345
VS D F E +C DI ++ L++AS ++ + + I + + L
Sbjct: 158 VSEDVFDVFME----NCGKFDRDDIYGLISLYEASYLSTKLDKNLQIFIRPFATQQLRDF 217
Query: 346 LSSNSIHSDRLHRNMKQEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNL 405
+ ++S + D +M + V+ ALD Y + RL + I+ Y N
Sbjct: 218 VDTHS-NEDFGSCDMVEIVVQALDMPYYWQMRRLSTRWYIDVYGKRQ-----------NY 277
Query: 406 GHQDFLRLAMEDFNVCQCIHRQEKKDLERWVVENKLDKLI--ARQKLVYCYFTAAAAITS 465
+ + A DFN+ Q IH++E K++ W +E L K + AR ++V YF I
Sbjct: 278 KNLVVVEFAKIDFNIVQAIHQEELKNVSSWWMETGLGKQLYFARDRIVENYFWTIGQIQE 337
Query: 466 PELSDARLSWAKNGVLTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIF 525
P+ R + K L T +DD++D++G+ +E A E WD+ N ++ E + + F
Sbjct: 338 PQYGYVRQTMTKINALLTTIDDIYDIYGTLEELQLFTVAFENWDI-NRLDELPEYMRLCF 397
Query: 526 KALHSTVDEIGNKALTWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGY 585
+++ V+ I + L + V + W ++ ++ EA+W + P++++YM+N
Sbjct: 398 LVIYNEVNSIACEILRTKNINVIPFLKKSWTDVSKAYLVEAKWYKSGHKPNLEEYMQNAR 457
Query: 586 ISFALGPIVLPALYFIGPKLSEEVVGSFEYH--NLFKLMSTCGRLLNDVQTIERESSEGK 645
IS + I + +LS +V+ + H N+ + S+ RL ND+ T E + G
Sbjct: 458 ISISSPTIFVHFYCVFSDQLSIQVLETLSQHQQNVVRCSSSVFRLANDLVTSPDELARGD 512
Query: 646 L-NALSLHMINTGGN 655
+ ++ +M TG +
Sbjct: 518 VCKSIQCYMSETGAS 512
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022147909.1 | 0.0e+00 | 73.33 | ent-kaur-16-ene synthase, chloroplastic isoform X2 [Momordica charantia] | [more] |
XP_022147908.1 | 0.0e+00 | 73.13 | ent-kaur-16-ene synthase, chloroplastic isoform X1 [Momordica charantia] | [more] |
XP_022147910.1 | 0.0e+00 | 73.13 | ent-kaur-16-ene synthase, chloroplastic isoform X3 [Momordica charantia] | [more] |
XP_016900364.1 | 0.0e+00 | 72.11 | PREDICTED: ent-kaur-16-ene synthase, chloroplastic isoform X2 [Cucumis melo] | [more] |
XP_008447073.1 | 0.0e+00 | 71.78 | PREDICTED: ent-kaur-16-ene synthase, chloroplastic isoform X1 [Cucumis melo] | [more] |
Match Name | E-value | Identity | Description | |
Q39548 | 3.4e-292 | 66.89 | Ent-kaur-16-ene synthase, chloroplastic OS=Cucurbita maxima OX=3661 PE=1 SV=1 | [more] |
A0A1Z3GC64 | 1.8e-237 | 54.90 | Ent-kaurene synthase 5, chloroplastic OS=Isodon rubescens OX=587669 GN=KSL5 PE=1... | [more] |
A0A2K9RFY0 | 3.1e-237 | 55.19 | Ent-kaurene synthase TSP4, chloroplastic OS=Vitex agnus-castus OX=54477 GN=TPS4 ... | [more] |
A0A0U4CDK4 | 1.6e-236 | 54.30 | Ent-kaur-16-ene synthase, chloroplastic OS=Salvia miltiorrhiza OX=226208 GN=KS P... | [more] |
A0A3G1QTS7 | 2.8e-233 | 54.22 | Ent-kaurene synthase 1, chloroplastic OS=Isodon eriocalyx OX=662907 GN=KS1 PE=1 ... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1D2F0 | 0.0e+00 | 73.33 | ent-kaur-16-ene synthase, chloroplastic isoform X2 OS=Momordica charantia OX=367... | [more] |
A0A6J1D2L1 | 0.0e+00 | 73.13 | ent-kaur-16-ene synthase, chloroplastic isoform X1 OS=Momordica charantia OX=367... | [more] |
A0A6J1D1G1 | 0.0e+00 | 73.13 | ent-kaur-16-ene synthase, chloroplastic isoform X3 OS=Momordica charantia OX=367... | [more] |
A0A1S4DWJ8 | 0.0e+00 | 72.11 | ent-kaur-16-ene synthase, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LO... | [more] |
A0A1S3BG06 | 0.0e+00 | 71.78 | ent-kaur-16-ene synthase, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LO... | [more] |