Sgr028675 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr028675
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptionent-kaurene synthase, chloroplastic
Locationtig00153204: 3392139 .. 3397408 (-)
RNA-Seq ExpressionSgr028675
SyntenySgr028675
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTGAAAAAGTTGAACTTTCTGTTTCCGCATATGATACTGCTTGGATGGCACTGGTCCCTACTCCAAACTGCCTTAAAACCCCTCTTTTTCCCGAGTGCATAAACTGGTTGCTGGATAATCAACTCCCTGATGGCTCCTGGGGCTTTCTTCATCCCCATCCCATCCTGACAAAGGCTACGCTCTTATCTACCTTAGCATGTCTTCTTCCTCTTAAGCGATGGGATATTGGTCAAGAGCATATGATCAAGGGTATTAATTTCTAATGTTCTTGCAGTTTCTTTTCCATTGAAATTTAACTTCTTCCTTCAAGTAGGCTTCAGTTGTCAAGTGAAAGGCATTTACCTTCTCATGCAGGCCTTAATTTTATTGAGTCAAATTTAGATTCGGCTACTGATGAAAAGCAACGTTCTCCTATTGGATTTGACATAATTTTCCCTGGCATGATCGAGTATGCTAAAACACTGGAGCTAAATTTACCATTGACACAAATGAATGTGGATGCGTTGATTCATAGGAGAGAATTGGAGCTTAATAGGTAATATAATCTATATCACGACCTTCTCCATTGGTAAACCTAAGAAATGTTGCATAACGACTTTTAGAAGTAGATCAGATTACATATGAATGAGAAATTTATAAGTGATCATTGCCAAAACCACAGTTCTATTTCCTTTGTACTAATCCATAAGGTGAATATTGGAAATTTTTTTCAATTATTTGGAGTTTAACACTGTCAAAATCGATATTCTCTTGCACCCTTTTGAGGAAATTTTTATCTTTATAAAATGTGTCAATGCGTCTTGTCATTTTTATGACTGTTTACTGTGAGTTCATATGAGAACCATCTCGATTTTACAATCTGTAAAAATTTTCATGATTTTATAGAAATGTTGTCACTTTTACAAACCAACCTGTAAACTCAGATTTTCAGATTGTGTGATATAGTTCAGAAAATTATTTCAAGGGGTAACAACTTATATAAAGCTGAAAGTTTGCATAAAACTCAAGCTCTATGCCCATCAATTTTATTTGAAGTTTCTGAAGTGGATTCTATATGCAACTTGATGGGTGATTTCACAGACCTGCCTTGTTCAAGCACTGGATAATTTCTGAAACTTTTTGTTATTTGTTGTATACTCAAGTTTTTGTATTTCCTTTTCTAGATTCTAACCTCATACTGCAATAAGAAATACAAATCTTTCATGATGGAGTGATCAATATAAGCTTAGATGGTTTTGCTTATAGATTTCACTATATTTCTCTGTGAAGTAGATAACTACTAAACTTTTGCTTTCTGTTAATTTTATATTCTTCTTACATATTGCTAGAGAAATTTTGGTACACTCACCTTAATGAATTAATGTAAGCATCCCAACTATTTCAAAAAGAAATAATCTACATCTTGAGGCACACTCATTTTATGCTAAATTTCATCTTAAATATGTCTTCTTCCTGGGGTACTATTTCCATATGCTTTCTAATTTCTACATCATGTCATTGACACATTAGACTGTATCAGCATTGTGCTCTGAAAGCACTCATAAAGCAAGTGGATTTAAGCTAATCCAATCTGACTGCCGTTGAGTTAATTAAGCTCTGGTATCCAGCTTATGCTTGGATTGATTTTCTTGAGTTGGCTCATAAATTTCTTTTCTTTGAAATACATGTGTGATCAATAATTTAATATGATGATCCTGTACTCAGGTTGAAACCTGTTGGGAGTTGATCATTCTTGCTTGTTCGGCTTAGCATGCTTGCTTAAACTTCAAACTATCGAAATGTAAACCAGAAAGTAGACAGTTACCTTTCTTTGCATATGAATGTTTTGCCACTTTAACCTCTTGATTTAAGAGACAACGTCTATATGCAGTGATGTATAGCGGTCTAGTACTGACTGTTTATCCATCTGCTCTGTCTCCTAATATTGTCCTGTTTTAGGGGCTGCAGAAGCAACTCTGAAGGGAGAAAAGCCTATTTGGCATATGTTTCAGAAGGAATCGGAAAGTCACAGGACTGGGACATGGTCATGAATTTTCAAAGAAAAAATGGTTCACTGTTTAATTCTCCATCCACAACAGCAGCGGCTTTTATGAACCTTAATAATGATAGTTGTCTCGATTACCTTCACTCTCTTTTACAGAAGTTCGGCAATGCAGGTTCCTTTGTCTTCAATTTTTGGCTTTCTTTCTCTCTCTTTTCATGTATATGTCAATTATTTGGCTGTAACTCTTTTGTGCTTGCTGCATTTCAGTTCCAACTATATATCCTCTAGATACATATGCTCAATTATGCATGGTTGATAGCCTTCAAACATTGGGTATAGATCATCATTTCAAAGAGGAAATTGGGAGAGTATTGGATAAAACTTATAGGTAGTGTAATATTTATAATGCCTGCATCAAATGATGAACTTAAAAATATTATACATAAATTGATTAGTATTTTTCTCATACAAAATGGTTGTGGTAATTTCTCCCAAACAACAGGTATTGGCTCCAAGGGGAGGAAAATATACTTTTAGATGCTTCAACCTGTGCAATGGCCTTCCGGATGTTACGTGTCTATGGATATGAGGTTTCTTCAGGTGCTTTATAATTTACTAAACTTGGTGCTGGGAATCTTATATCTAAACTATGATCTTATTGGTTTACATTTTTTATTCTTTAGATCAGTTGGCTCAATTTTCAGAGGATCGTTTTTCTGATTGCCTTGGAGGATATCTAAAGGATATAGGCTCTGTACTGGAATTATTCAAGGCTTCTCAAATAACCATTTATCCAGAGGATCCTGTTCTGGAAAACATCAACTCTTGGACAAGTCATTTTTTGAATTATGGATTATCAAGTAATTCAATTCATTCTGATAGACTTCATAGAAACATGAAACAAGAGGTACCCCTGTCCCAGAATAATCCGATACAAATCAAGTAGAAGTCTCGCTTAGATCCTTTCTTTTTCTTCCCCTTATATATAGACTGAAACAGAACTTCCTGGAAACTATCAACTTTGGTCAATTTCAGGTGATTAGTGCTCTCGACTTTACCTACAATACAACATTGGAGCGGTTACAATATAAGAGTGCTATTGAACATTACAGCGTAAACAACATAAGGATTTTAAAAACTCCATATAGGCATGACCAATCAAGTCTTCTGCTTTTTCTTTTTCATCTTTTGTCATCTGTCTTGGTTGAACGTGAACCACAGTTTATAAATACAACTACTTAATTCTAGTTCTTATTTCAGCTGCTTAAATCTTGGACATCAAGATTTTCTAAGATTAGCTATGGAAGATTTCAACGTCTGTCAATGCATACATCGTCAAGAAAAGAAAGATCTTGAAAGGTAAATTGTAGCTACTGACATTGTTCATACAGTCTTGATCATGGCAAGATCTAGCCTTGATCCCTTCTAAAATTTTTTACTTTGTGATCCAAGGACCAGGTTGCTAATGCAAATAAATGGTCTAAGTAACAGCTGGGTCATTGATGAATTTGTATGCAGATATTAAATAATGGGGAATTTTACTAAGAGGTAGTAGCAATTGGGAAAAAAGTGAAAATTAGTGAAATCATGATTTGTTTTTCTCCTCGTCCTATTAATAGTTCCAAATAAATAATTAATAAATCTAAAAGAAGGGACTATTGAGCCAATGCCTATTTTTATAAGTTCGTTTACCCTCTTTTACCCAGGTGCTGTCTATTTACATCAGAGCAAATGTTTTATGTAGAAGGCAATATTCTTTAACATATACAAATCATTGCTTCTATTTCATTTCAATATTCTCTTTTCTCTACTCCTTCAGGTCAGCCTTACAAGTTGGCCTCCATTTTGCATGTAATTAAGTCATTTAGATTGTCTTAGGTTCTCTGTTGTTGTACAATACAATACACAAAAATAATTCTCCCCTGTCAATCCTACTTCCATTTCATATGAATAGGTGGGTTGTTGAAAACAAATTGGACAAGCTGATTGCAAGACAGAAGCTAGTTTACTGCTATTTTACTGCTGCGGCAGCTATAACCTCTCCTGAACTTTCTGACGCCCGCTTGTCATGGGCCAAAAATGGGGTGCTCACAACTGTGGTTGACGATTTATTTGATGTTTGGGGATCTGAAGATGAAAGGATAAACCTTGTACAAGCGGTAGAAAGGTATGTCTCCTTTTTGCTTCCCTTCATTATTATTTTGCTCAAGCAAGTTTTCTAGTTTGAAAACCACCTCTTCTTAAATTCTTCCTAGCATGAAGCTGATAATTCCATGAGAAATAAACTGCAGGTGGGATGTTTGTAATGACATGGAGTACTGTTCTGAGAAGGTTGAGATAATATTCAAAGCACTTCACAGCACAGTTGATGAAATTGGCAACAAAGCATTGACATGGCAAGGACGTTGCGTGACGAGCCATGTAGCTGACATTGTAAGATGTTCGCGATACTCATCTTTATGACCTTTAGTAATTATCAGAATGCTTGCTTGTAGCATTGCTAGTACTACCTGTGTTTTGAGAACATAGCGTGCCTCATTTTATACTGTTTGGGCATTGATGCTGGAAATCCACATCAGATGTTCTTGATCCGTCATCTAAATGTGCTACTTAACAAAATATGACGTGCACCATGCTTCATTTGCAGTGGTTGGAATTGATCAGATCTATGAGACAGGAAGCTGAGTGGTTGAGTAATAAGGCAGTGCCATCAATGGATGATTATATGGAAAATGGCTATATATCATTTGCCTTAGGACCTATAGTCCTTCCAGCGCTATATTTTATTGGGCCTAAACTTTCAGAGGAGGTGGTGGGCAGTTTTGAGTACCATAATCTATTTAAGCTGATGAGCACTTGTGGTCGCCTTCTCAACGACGTTCAAACTATTGAGGTACTTCCTCGAACAAGTCAAATTATCTAATTTTGTGTGTTAAGAGTTGTTGATCATTCCTGGTTACATCACAGAGGGAAAGCAGTGAAGGAAAACTGAATGCTCTTTCGTTGCACATGATTAATACTGGTGGTAATAGAACGAATGAGGAGGTCATTAGGGCAATTGAAGTTGATATTGACAAATCTAGACGAGAACTGCTGAGATTAGTTTTGCAGGAGAAAGATAGTACAATTCCCAGAGCTTGCAAGGATCTGTTCTGGAGAATGAGCTGTGTGGTGCACCTATTTTACCGGAAGGACGACGGGTTTACTTCACTTGGGTTGATGAATTCTGCAAAAGCATTATTTGAAGAACCCATAAGTTTTGATAACCTTTGGAGTGGATGA

mRNA sequence

ATGTTTGAAAAAGTTGAACTTTCTGTTTCCGCATATGATACTGCTTGGATGGCACTGGTCCCTACTCCAAACTGCCTTAAAACCCCTCTTTTTCCCGAGTGCATAAACTGGTTGCTGGATAATCAACTCCCTGATGGCTCCTGGGGCTTTCTTCATCCCCATCCCATCCTGACAAAGGCTACGCTCTTATCTACCTTAGCATGTCTTCTTCCTCTTAAGCGATGGGATATTGGTCAAGAGCATATGATCAAGGGCCTTAATTTTATTGAGTCAAATTTAGATTCGGCTACTGATGAAAAGCAACGTTCTCCTATTGGATTTGACATAATTTTCCCTGGCATGATCGAGTATGCTAAAACACTGGAGCTAAATTTACCATTGACACAAATGAATGTGGATGCGTTGATTCATAGGAGAGAATTGGAGCTTAATAGAAGCAACTCTGAAGGGAGAAAAGCCTATTTGGCATATGTTTCAGAAGGAATCGGAAAGTCACAGGACTGGGACATGGTCATGAATTTTCAAAGAAAAAATGGTTCACTGTTTAATTCTCCATCCACAACAGCAGCGGCTTTTATGAACCTTAATAATGATAGTTGTCTCGATTACCTTCACTCTCTTTTACAGAAGTTCGGCAATGCAGTTCCAACTATATATCCTCTAGATACATATGCTCAATTATGCATGGTTGATAGCCTTCAAACATTGGGTATAGATCATCATTTCAAAGAGGAAATTGGGAGAGTATTGGATAAAACTTATAGGTATTGGCTCCAAGGGGAGGAAAATATACTTTTAGATGCTTCAACCTGTGCAATGGCCTTCCGGATGTTACGTGTCTATGGATATGAGGTTTCTTCAGATCAGTTGGCTCAATTTTCAGAGGATCGTTTTTCTGATTGCCTTGGAGGATATCTAAAGGATATAGGCTCTGTACTGGAATTATTCAAGGCTTCTCAAATAACCATTTATCCAGAGGATCCTGTTCTGGAAAACATCAACTCTTGGACAAGTCATTTTTTGAATTATGGATTATCAAGTAATTCAATTCATTCTGATAGACTTCATAGAAACATGAAACAAGAGGTGATTAGTGCTCTCGACTTTACCTACAATACAACATTGGAGCGGTTACAATATAAGAGTGCTATTGAACATTACAGCGTAAACAACATAAGGATTTTAAAAACTCCATATAGCTGCTTAAATCTTGGACATCAAGATTTTCTAAGATTAGCTATGGAAGATTTCAACGTCTGTCAATGCATACATCGTCAAGAAAAGAAAGATCTTGAAAGGTGGGTTGTTGAAAACAAATTGGACAAGCTGATTGCAAGACAGAAGCTAGTTTACTGCTATTTTACTGCTGCGGCAGCTATAACCTCTCCTGAACTTTCTGACGCCCGCTTGTCATGGGCCAAAAATGGGGTGCTCACAACTGTGGTTGACGATTTATTTGATGTTTGGGGATCTGAAGATGAAAGGATAAACCTTGTACAAGCGGTAGAAAGGTGGGATGTTTGTAATGACATGGAGTACTGTTCTGAGAAGGTTGAGATAATATTCAAAGCACTTCACAGCACAGTTGATGAAATTGGCAACAAAGCATTGACATGGCAAGGACGTTGCGTGACGAGCCATGTAGCTGACATTTGGTTGGAATTGATCAGATCTATGAGACAGGAAGCTGAGTGGTTGAGTAATAAGGCAGTGCCATCAATGGATGATTATATGGAAAATGGCTATATATCATTTGCCTTAGGACCTATAGTCCTTCCAGCGCTATATTTTATTGGGCCTAAACTTTCAGAGGAGGTGGTGGGCAGTTTTGAGTACCATAATCTATTTAAGCTGATGAGCACTTGTGGTCGCCTTCTCAACGACGTTCAAACTATTGAGAGGGAAAGCAGTGAAGGAAAACTGAATGCTCTTTCGTTGCACATGATTAATACTGGTGGTAATAGAACGAATGAGGAGGTCATTAGGGCAATTGAAGTTGATATTGACAAATCTAGACGAGAACTGCTGAGATTAGTTTTGCAGGAGAAAGATAGTACAATTCCCAGAGCTTGCAAGGATCTGTTCTGGAGAATGAGCTGTGTGGTGCACCTATTTTACCGGAAGGACGACGGGTTTACTTCACTTGGGTTGATGAATTCTGCAAAAGCATTATTTGAAGAACCCATAAGTTTTGATAACCTTTGGAGTGGATGA

Coding sequence (CDS)

ATGTTTGAAAAAGTTGAACTTTCTGTTTCCGCATATGATACTGCTTGGATGGCACTGGTCCCTACTCCAAACTGCCTTAAAACCCCTCTTTTTCCCGAGTGCATAAACTGGTTGCTGGATAATCAACTCCCTGATGGCTCCTGGGGCTTTCTTCATCCCCATCCCATCCTGACAAAGGCTACGCTCTTATCTACCTTAGCATGTCTTCTTCCTCTTAAGCGATGGGATATTGGTCAAGAGCATATGATCAAGGGCCTTAATTTTATTGAGTCAAATTTAGATTCGGCTACTGATGAAAAGCAACGTTCTCCTATTGGATTTGACATAATTTTCCCTGGCATGATCGAGTATGCTAAAACACTGGAGCTAAATTTACCATTGACACAAATGAATGTGGATGCGTTGATTCATAGGAGAGAATTGGAGCTTAATAGAAGCAACTCTGAAGGGAGAAAAGCCTATTTGGCATATGTTTCAGAAGGAATCGGAAAGTCACAGGACTGGGACATGGTCATGAATTTTCAAAGAAAAAATGGTTCACTGTTTAATTCTCCATCCACAACAGCAGCGGCTTTTATGAACCTTAATAATGATAGTTGTCTCGATTACCTTCACTCTCTTTTACAGAAGTTCGGCAATGCAGTTCCAACTATATATCCTCTAGATACATATGCTCAATTATGCATGGTTGATAGCCTTCAAACATTGGGTATAGATCATCATTTCAAAGAGGAAATTGGGAGAGTATTGGATAAAACTTATAGGTATTGGCTCCAAGGGGAGGAAAATATACTTTTAGATGCTTCAACCTGTGCAATGGCCTTCCGGATGTTACGTGTCTATGGATATGAGGTTTCTTCAGATCAGTTGGCTCAATTTTCAGAGGATCGTTTTTCTGATTGCCTTGGAGGATATCTAAAGGATATAGGCTCTGTACTGGAATTATTCAAGGCTTCTCAAATAACCATTTATCCAGAGGATCCTGTTCTGGAAAACATCAACTCTTGGACAAGTCATTTTTTGAATTATGGATTATCAAGTAATTCAATTCATTCTGATAGACTTCATAGAAACATGAAACAAGAGGTGATTAGTGCTCTCGACTTTACCTACAATACAACATTGGAGCGGTTACAATATAAGAGTGCTATTGAACATTACAGCGTAAACAACATAAGGATTTTAAAAACTCCATATAGCTGCTTAAATCTTGGACATCAAGATTTTCTAAGATTAGCTATGGAAGATTTCAACGTCTGTCAATGCATACATCGTCAAGAAAAGAAAGATCTTGAAAGGTGGGTTGTTGAAAACAAATTGGACAAGCTGATTGCAAGACAGAAGCTAGTTTACTGCTATTTTACTGCTGCGGCAGCTATAACCTCTCCTGAACTTTCTGACGCCCGCTTGTCATGGGCCAAAAATGGGGTGCTCACAACTGTGGTTGACGATTTATTTGATGTTTGGGGATCTGAAGATGAAAGGATAAACCTTGTACAAGCGGTAGAAAGGTGGGATGTTTGTAATGACATGGAGTACTGTTCTGAGAAGGTTGAGATAATATTCAAAGCACTTCACAGCACAGTTGATGAAATTGGCAACAAAGCATTGACATGGCAAGGACGTTGCGTGACGAGCCATGTAGCTGACATTTGGTTGGAATTGATCAGATCTATGAGACAGGAAGCTGAGTGGTTGAGTAATAAGGCAGTGCCATCAATGGATGATTATATGGAAAATGGCTATATATCATTTGCCTTAGGACCTATAGTCCTTCCAGCGCTATATTTTATTGGGCCTAAACTTTCAGAGGAGGTGGTGGGCAGTTTTGAGTACCATAATCTATTTAAGCTGATGAGCACTTGTGGTCGCCTTCTCAACGACGTTCAAACTATTGAGAGGGAAAGCAGTGAAGGAAAACTGAATGCTCTTTCGTTGCACATGATTAATACTGGTGGTAATAGAACGAATGAGGAGGTCATTAGGGCAATTGAAGTTGATATTGACAAATCTAGACGAGAACTGCTGAGATTAGTTTTGCAGGAGAAAGATAGTACAATTCCCAGAGCTTGCAAGGATCTGTTCTGGAGAATGAGCTGTGTGGTGCACCTATTTTACCGGAAGGACGACGGGTTTACTTCACTTGGGTTGATGAATTCTGCAAAAGCATTATTTGAAGAACCCATAAGTTTTGATAACCTTTGGAGTGGATGA

Protein sequence

MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKATLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKTLELNLPLTQMNVDALIHRRELELNRSNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRKNGSLFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLGIDHHFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDRFSDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRLHRNMKQEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMEDFNVCQCIHRQEKKDLERWVVENKLDKLIARQKLVYCYFTAAAAITSPELSDARLSWAKNGVLTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKALTWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPALYFIGPKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNRTNEEVIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSLGLMNSAKALFEEPISFDNLWSG
Homology
BLAST of Sgr028675 vs. NCBI nr
Match: XP_022147909.1 (ent-kaur-16-ene synthase, chloroplastic isoform X2 [Momordica charantia])

HSP 1 Score: 1117.4 bits (2889), Expect = 0.0e+00
Identity = 539/735 (73.33%), Postives = 625/735 (85.03%), Query Frame = 0

Query: 1   MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
           MF KVELSVSAYDTAW+A+VP+PN L  PLFPECINW+LDNQ PDGSWG +H H +L KA
Sbjct: 96  MFNKVELSVSAYDTAWVAMVPSPNSLNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKA 155

Query: 61  TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
           +LLSTLAC L LKRW+IG EHM K LNFI+SN+ SATDEKQ SP+GFDIIFPGMIEYAK 
Sbjct: 156 SLLSTLACALTLKRWNIGHEHMSKALNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKD 215

Query: 121 LELNLPLTQMNVDALIHRRELELNRSNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRKNGS 180
           L+LNLPL   NVDAL+ ++EL+L RSNSEG KAYLAYVSEGIG+ +DW+MVM +QRKNGS
Sbjct: 216 LDLNLPLAPANVDALLQKKELDLRRSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKNGS 275

Query: 181 LFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLGIDH 240
           LFNSPSTTA AFM+ N+D C +YLHSLLQKF N+VPTIYPLD YA+LCMVD+LQ LGID 
Sbjct: 276 LFNSPSTTAVAFMHGNDDGCFNYLHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGIDR 335

Query: 241 HFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDRFSD 300
           HFKEEI  VLD+TYR W+QGEE+I LDASTCAMAFRMLR+ GY+VSSDQL QFSED FS+
Sbjct: 336 HFKEEIRSVLDETYRCWMQGEEDIFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLFSN 395

Query: 301 CLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRLHRNMK 360
           CLGGYLKD GS LELFKASQI IYP++ +LENINSWT+ FL++GLSS S+HSDR  R +K
Sbjct: 396 CLGGYLKDFGSSLELFKASQIIIYPDESILENINSWTNSFLSHGLSSGSVHSDRPDRLLK 455

Query: 361 QEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMEDFNVC 420
           QE I+AL+FTY+ TLERL  K A+E+Y+++ +RI KT Y+CLN GHQDFL LA+EDFN+ 
Sbjct: 456 QEAINALEFTYSNTLERLLNKRAMENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFNIL 515

Query: 421 QCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKNGVLT 480
           Q IH +E K+LERWVVEN+LDKL  ARQKL YCYF+AAA +TSPEL DARLSWAKNGVLT
Sbjct: 516 QSIHHKELKELERWVVENRLDKLKFARQKLAYCYFSAAATLTSPELRDARLSWAKNGVLT 575

Query: 481 TVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKALTW 540
           TVVDD FDV GSE E INL+Q VE+WDV  + +YCS++VEIIF ALH TV EI  +AL W
Sbjct: 576 TVVDDFFDVGGSEGELINLIQLVEKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRALPW 635

Query: 541 QGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPALYFIG 600
           QGR V  +V DIWL L++SMR+EAEWL NK  P MD+YMENGYISFALGPIVLPAL+F+G
Sbjct: 636 QGRSVMRNVIDIWLALLKSMRKEAEWLKNKIAPPMDEYMENGYISFALGPIVLPALFFVG 695

Query: 601 PKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNRTNEE 660
           PKL EEV+GS EYH LFKLMSTCGRLLND Q+ ERESSEGKLNA+SL MIN GG+ T EE
Sbjct: 696 PKLPEEVIGSCEYHKLFKLMSTCGRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTKEE 755

Query: 661 VIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSLGLMN 720
              AI+ +++++RRELLRLVLQEK+ST+PRACKDLFW+MSCVVHLFYRKDDGFTS  LMN
Sbjct: 756 ASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHELMN 815

Query: 721 SAKALFEEPISFDNL 735
           SAKAL E+P+  D L
Sbjct: 816 SAKALLEQPMVLDEL 830

BLAST of Sgr028675 vs. NCBI nr
Match: XP_022147908.1 (ent-kaur-16-ene synthase, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 1112.4 bits (2876), Expect = 0.0e+00
Identity = 539/737 (73.13%), Postives = 625/737 (84.80%), Query Frame = 0

Query: 1   MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
           MF KVELSVSAYDTAW+A+VP+PN L  PLFPECINW+LDNQ PDGSWG +H H +L KA
Sbjct: 96  MFNKVELSVSAYDTAWVAMVPSPNSLNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKA 155

Query: 61  TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
           +LLSTLAC L LKRW+IG EHM K LNFI+SN+ SATDEKQ SP+GFDIIFPGMIEYAK 
Sbjct: 156 SLLSTLACALTLKRWNIGHEHMSKALNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKD 215

Query: 121 LELNLPLTQMNVDALIHRRELELN--RSNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRKN 180
           L+LNLPL   NVDAL+ ++EL+L   RSNSEG KAYLAYVSEGIG+ +DW+MVM +QRKN
Sbjct: 216 LDLNLPLAPANVDALLQKKELDLRSCRSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKN 275

Query: 181 GSLFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLGI 240
           GSLFNSPSTTA AFM+ N+D C +YLHSLLQKF N+VPTIYPLD YA+LCMVD+LQ LGI
Sbjct: 276 GSLFNSPSTTAVAFMHGNDDGCFNYLHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGI 335

Query: 241 DHHFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDRF 300
           D HFKEEI  VLD+TYR W+QGEE+I LDASTCAMAFRMLR+ GY+VSSDQL QFSED F
Sbjct: 336 DRHFKEEIRSVLDETYRCWMQGEEDIFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLF 395

Query: 301 SDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRLHRN 360
           S+CLGGYLKD GS LELFKASQI IYP++ +LENINSWT+ FL++GLSS S+HSDR  R 
Sbjct: 396 SNCLGGYLKDFGSSLELFKASQIIIYPDESILENINSWTNSFLSHGLSSGSVHSDRPDRL 455

Query: 361 MKQEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMEDFN 420
           +KQE I+AL+FTY+ TLERL  K A+E+Y+++ +RI KT Y+CLN GHQDFL LA+EDFN
Sbjct: 456 LKQEAINALEFTYSNTLERLLNKRAMENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFN 515

Query: 421 VCQCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKNGV 480
           + Q IH +E K+LERWVVEN+LDKL  ARQKL YCYF+AAA +TSPEL DARLSWAKNGV
Sbjct: 516 ILQSIHHKELKELERWVVENRLDKLKFARQKLAYCYFSAAATLTSPELRDARLSWAKNGV 575

Query: 481 LTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKAL 540
           LTTVVDD FDV GSE E INL+Q VE+WDV  + +YCS++VEIIF ALH TV EI  +AL
Sbjct: 576 LTTVVDDFFDVGGSEGELINLIQLVEKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRAL 635

Query: 541 TWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPALYF 600
            WQGR V  +V DIWL L++SMR+EAEWL NK  P MD+YMENGYISFALGPIVLPAL+F
Sbjct: 636 PWQGRSVMRNVIDIWLALLKSMRKEAEWLKNKIAPPMDEYMENGYISFALGPIVLPALFF 695

Query: 601 IGPKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNRTN 660
           +GPKL EEV+GS EYH LFKLMSTCGRLLND Q+ ERESSEGKLNA+SL MIN GG+ T 
Sbjct: 696 VGPKLPEEVIGSCEYHKLFKLMSTCGRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTK 755

Query: 661 EEVIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSLGL 720
           EE   AI+ +++++RRELLRLVLQEK+ST+PRACKDLFW+MSCVVHLFYRKDDGFTS  L
Sbjct: 756 EEASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHEL 815

Query: 721 MNSAKALFEEPISFDNL 735
           MNSAKAL E+P+  D L
Sbjct: 816 MNSAKALLEQPMVLDEL 832

BLAST of Sgr028675 vs. NCBI nr
Match: XP_022147910.1 (ent-kaur-16-ene synthase, chloroplastic isoform X3 [Momordica charantia])

HSP 1 Score: 1112.4 bits (2876), Expect = 0.0e+00
Identity = 539/737 (73.13%), Postives = 625/737 (84.80%), Query Frame = 0

Query: 1   MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
           MF KVELSVSAYDTAW+A+VP+PN L  PLFPECINW+LDNQ PDGSWG +H H +L KA
Sbjct: 83  MFNKVELSVSAYDTAWVAMVPSPNSLNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKA 142

Query: 61  TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
           +LLSTLAC L LKRW+IG EHM K LNFI+SN+ SATDEKQ SP+GFDIIFPGMIEYAK 
Sbjct: 143 SLLSTLACALTLKRWNIGHEHMSKALNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKD 202

Query: 121 LELNLPLTQMNVDALIHRRELELN--RSNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRKN 180
           L+LNLPL   NVDAL+ ++EL+L   RSNSEG KAYLAYVSEGIG+ +DW+MVM +QRKN
Sbjct: 203 LDLNLPLAPANVDALLQKKELDLRSCRSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKN 262

Query: 181 GSLFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLGI 240
           GSLFNSPSTTA AFM+ N+D C +YLHSLLQKF N+VPTIYPLD YA+LCMVD+LQ LGI
Sbjct: 263 GSLFNSPSTTAVAFMHGNDDGCFNYLHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGI 322

Query: 241 DHHFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDRF 300
           D HFKEEI  VLD+TYR W+QGEE+I LDASTCAMAFRMLR+ GY+VSSDQL QFSED F
Sbjct: 323 DRHFKEEIRSVLDETYRCWMQGEEDIFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLF 382

Query: 301 SDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRLHRN 360
           S+CLGGYLKD GS LELFKASQI IYP++ +LENINSWT+ FL++GLSS S+HSDR  R 
Sbjct: 383 SNCLGGYLKDFGSSLELFKASQIIIYPDESILENINSWTNSFLSHGLSSGSVHSDRPDRL 442

Query: 361 MKQEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMEDFN 420
           +KQE I+AL+FTY+ TLERL  K A+E+Y+++ +RI KT Y+CLN GHQDFL LA+EDFN
Sbjct: 443 LKQEAINALEFTYSNTLERLLNKRAMENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFN 502

Query: 421 VCQCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKNGV 480
           + Q IH +E K+LERWVVEN+LDKL  ARQKL YCYF+AAA +TSPEL DARLSWAKNGV
Sbjct: 503 ILQSIHHKELKELERWVVENRLDKLKFARQKLAYCYFSAAATLTSPELRDARLSWAKNGV 562

Query: 481 LTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKAL 540
           LTTVVDD FDV GSE E INL+Q VE+WDV  + +YCS++VEIIF ALH TV EI  +AL
Sbjct: 563 LTTVVDDFFDVGGSEGELINLIQLVEKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRAL 622

Query: 541 TWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPALYF 600
            WQGR V  +V DIWL L++SMR+EAEWL NK  P MD+YMENGYISFALGPIVLPAL+F
Sbjct: 623 PWQGRSVMRNVIDIWLALLKSMRKEAEWLKNKIAPPMDEYMENGYISFALGPIVLPALFF 682

Query: 601 IGPKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNRTN 660
           +GPKL EEV+GS EYH LFKLMSTCGRLLND Q+ ERESSEGKLNA+SL MIN GG+ T 
Sbjct: 683 VGPKLPEEVIGSCEYHKLFKLMSTCGRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTK 742

Query: 661 EEVIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSLGL 720
           EE   AI+ +++++RRELLRLVLQEK+ST+PRACKDLFW+MSCVVHLFYRKDDGFTS  L
Sbjct: 743 EEASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHEL 802

Query: 721 MNSAKALFEEPISFDNL 735
           MNSAKAL E+P+  D L
Sbjct: 803 MNSAKALLEQPMVLDEL 819

BLAST of Sgr028675 vs. NCBI nr
Match: XP_016900364.1 (PREDICTED: ent-kaur-16-ene synthase, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 530/735 (72.11%), Postives = 618/735 (84.08%), Query Frame = 0

Query: 1   MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
           +F+ VELSVSAYDTAW+A+VP+PN L  PLFPECINW+LD+Q PDGSWG LH H ++ KA
Sbjct: 47  LFKNVELSVSAYDTAWVAMVPSPNSLNKPLFPECINWVLDHQNPDGSWGILHDHQLVMKA 106

Query: 61  TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
           TLLSTLAC+L LKRW+IG +HM K L+FI+SN+ SATDE QRSP+GFDIIFPGMIEYAK 
Sbjct: 107 TLLSTLACVLTLKRWNIGHDHMSKALSFIKSNIASATDENQRSPVGFDIIFPGMIEYAKD 166

Query: 121 LELNLPLTQMNVDALIHRRELELNRSNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRKNGS 180
           L LNLPL  MNVDAL+ ++ELEL RSNSEG KAYLAYVSEGIGK QDWDMVM +QRKNGS
Sbjct: 167 LNLNLPLASMNVDALVQKKELEL-RSNSEGGKAYLAYVSEGIGKLQDWDMVMQYQRKNGS 226

Query: 181 LFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLGIDH 240
           LF+SPSTTA AFM+ N+D C +YL S+LQKF ++VP IYPLD YA+L MVDSLQ LGID 
Sbjct: 227 LFSSPSTTAVAFMHRNDDGCFNYLRSILQKFHSSVPAIYPLDIYARLHMVDSLQKLGIDR 286

Query: 241 HFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDRFSD 300
           HFK+EI  VLD+TYR W+QGEENI LDASTCAMAFRMLRV GY+VSSDQL QFSED F +
Sbjct: 287 HFKDEIRSVLDETYRCWMQGEENIFLDASTCAMAFRMLRVEGYDVSSDQLTQFSEDLFPN 346

Query: 301 CLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRLHRNMK 360
           CLGG+LKD G+ LELFKASQI IYP++ +LENINSWTS FLN+GLS  S+HSDR  R +K
Sbjct: 347 CLGGHLKDFGASLELFKASQIIIYPDEFILENINSWTSRFLNHGLSGGSVHSDRTERLVK 406

Query: 361 QEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMEDFNVC 420
           QE ++A +F YN+TLERL  K A+E YS + +RI KT Y+CLN GHQDFL LA+EDFN  
Sbjct: 407 QEAVNAFEFPYNSTLERLSNKRALESYSGDIVRISKTTYACLNFGHQDFLELAVEDFNTL 466

Query: 421 QCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKNGVLT 480
           QCIHR+E K+LE+WV+ENKLDKL  ARQKL YCYF+AAA +TSPEL DARLSWAKNGVLT
Sbjct: 467 QCIHRKELKELEKWVIENKLDKLKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGVLT 526

Query: 481 TVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKALTW 540
           TVVDD FDV GSE+E +NL+Q VE+WD   +  YCS++VEIIF ALHST+ EIG KAL W
Sbjct: 527 TVVDDFFDVGGSEEELVNLIQLVEKWDASGEAGYCSKEVEIIFLALHSTICEIGEKALPW 586

Query: 541 QGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPALYFIG 600
           QGR VT +V DIWL L+ SMR+EAEWL NK VPS+D+YMENGY+SFALGPIVLP LYF+G
Sbjct: 587 QGRSVTRNVIDIWLALLESMRKEAEWLKNKVVPSLDEYMENGYVSFALGPIVLPTLYFVG 646

Query: 601 PKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNRTNEE 660
           PKL EE++G+ EY  LFKLMST GRLLND +T +RESSEGKLNALSL++I+ GG  + EE
Sbjct: 647 PKLPEEIIGNCEYQKLFKLMSTSGRLLNDTRTFDRESSEGKLNALSLYLISAGGKLSKEE 706

Query: 661 VIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSLGLMN 720
              A++ D+D++RRELLRLVLQE +STIPRACKDLFW+MSCVVHLFYRKDDGFTS  LMN
Sbjct: 707 ATEAMKGDVDRTRRELLRLVLQE-NSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHELMN 766

Query: 721 SAKALFEEPISFDNL 735
           SAKALFE P+  D L
Sbjct: 767 SAKALFELPMVLDEL 779

BLAST of Sgr028675 vs. NCBI nr
Match: XP_008447073.1 (PREDICTED: ent-kaur-16-ene synthase, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 529/737 (71.78%), Postives = 617/737 (83.72%), Query Frame = 0

Query: 1   MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
           +F+ VELSVSAYDTAW+A+VP+PN L  PLFPECINW+LD+Q PDGSWG LH H ++ KA
Sbjct: 47  LFKNVELSVSAYDTAWVAMVPSPNSLNKPLFPECINWVLDHQNPDGSWGILHDHQLVMKA 106

Query: 61  TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
           TLLSTLAC+L LKRW+IG +HM K L+FI+SN+ SATDE QRSP+GFDIIFPGMIEYAK 
Sbjct: 107 TLLSTLACVLTLKRWNIGHDHMSKALSFIKSNIASATDENQRSPVGFDIIFPGMIEYAKD 166

Query: 121 LELNLPLTQMNVDALIHRRELELNR--SNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRKN 180
           L LNLPL  MNVDAL+ ++ELEL    SNSEG KAYLAYVSEGIGK QDWDMVM +QRKN
Sbjct: 167 LNLNLPLASMNVDALVQKKELELRSCCSNSEGGKAYLAYVSEGIGKLQDWDMVMQYQRKN 226

Query: 181 GSLFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLGI 240
           GSLF+SPSTTA AFM+ N+D C +YL S+LQKF ++VP IYPLD YA+L MVDSLQ LGI
Sbjct: 227 GSLFSSPSTTAVAFMHRNDDGCFNYLRSILQKFHSSVPAIYPLDIYARLHMVDSLQKLGI 286

Query: 241 DHHFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDRF 300
           D HFK+EI  VLD+TYR W+QGEENI LDASTCAMAFRMLRV GY+VSSDQL QFSED F
Sbjct: 287 DRHFKDEIRSVLDETYRCWMQGEENIFLDASTCAMAFRMLRVEGYDVSSDQLTQFSEDLF 346

Query: 301 SDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRLHRN 360
            +CLGG+LKD G+ LELFKASQI IYP++ +LENINSWTS FLN+GLS  S+HSDR  R 
Sbjct: 347 PNCLGGHLKDFGASLELFKASQIIIYPDEFILENINSWTSRFLNHGLSGGSVHSDRTERL 406

Query: 361 MKQEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMEDFN 420
           +KQE ++A +F YN+TLERL  K A+E YS + +RI KT Y+CLN GHQDFL LA+EDFN
Sbjct: 407 VKQEAVNAFEFPYNSTLERLSNKRALESYSGDIVRISKTTYACLNFGHQDFLELAVEDFN 466

Query: 421 VCQCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKNGV 480
             QCIHR+E K+LE+WV+ENKLDKL  ARQKL YCYF+AAA +TSPEL DARLSWAKNGV
Sbjct: 467 TLQCIHRKELKELEKWVIENKLDKLKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGV 526

Query: 481 LTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKAL 540
           LTTVVDD FDV GSE+E +NL+Q VE+WD   +  YCS++VEIIF ALHST+ EIG KAL
Sbjct: 527 LTTVVDDFFDVGGSEEELVNLIQLVEKWDASGEAGYCSKEVEIIFLALHSTICEIGEKAL 586

Query: 541 TWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPALYF 600
            WQGR VT +V DIWL L+ SMR+EAEWL NK VPS+D+YMENGY+SFALGPIVLP LYF
Sbjct: 587 PWQGRSVTRNVIDIWLALLESMRKEAEWLKNKVVPSLDEYMENGYVSFALGPIVLPTLYF 646

Query: 601 IGPKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNRTN 660
           +GPKL EE++G+ EY  LFKLMST GRLLND +T +RESSEGKLNALSL++I+ GG  + 
Sbjct: 647 VGPKLPEEIIGNCEYQKLFKLMSTSGRLLNDTRTFDRESSEGKLNALSLYLISAGGKLSK 706

Query: 661 EEVIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSLGL 720
           EE   A++ D+D++RRELLRLVLQE +STIPRACKDLFW+MSCVVHLFYRKDDGFTS  L
Sbjct: 707 EEATEAMKGDVDRTRRELLRLVLQE-NSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHEL 766

Query: 721 MNSAKALFEEPISFDNL 735
           MNSAKALFE P+  D L
Sbjct: 767 MNSAKALFELPMVLDEL 782

BLAST of Sgr028675 vs. ExPASy Swiss-Prot
Match: Q39548 (Ent-kaur-16-ene synthase, chloroplastic OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 1005.4 bits (2598), Expect = 3.4e-292
Identity = 493/737 (66.89%), Postives = 592/737 (80.33%), Query Frame = 0

Query: 1   MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
           +F+KVELSVSAYDTAW+A+VP+PN L  PLFPECINW+LD+Q  DGSWG LH   +L KA
Sbjct: 51  LFDKVELSVSAYDTAWVAMVPSPNSLNQPLFPECINWVLDSQHADGSWGLLHNDQLLMKA 110

Query: 61  TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
            LLSTLAC+L LKRW+IG +HM K L+FI+SN+ SATDE QRSP+GFDIIFPGMIEYAK 
Sbjct: 111 NLLSTLACVLTLKRWNIGHDHMSKALDFIKSNIASATDENQRSPVGFDIIFPGMIEYAKD 170

Query: 121 LELNLPLTQMNVDALIHRRELELN--RSNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRKN 180
           L LNLPL   NVDAL+ ++ELEL   RSNSEG KAYLAYVSEGIGK QDWDMVM +QRKN
Sbjct: 171 LNLNLPLAPTNVDALVRKKELELRSCRSNSEGGKAYLAYVSEGIGKLQDWDMVMQYQRKN 230

Query: 181 GSLFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLGI 240
           GSLFNSPSTTAAAFM+ N+D C DYL SLLQKF  +VPTIYPLD YA+L MVDSLQ  GI
Sbjct: 231 GSLFNSPSTTAAAFMHRNDDGCFDYLRSLLQKFDGSVPTIYPLDIYARLHMVDSLQKFGI 290

Query: 241 DHHFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDRF 300
             HFKEEI  VLD+TYR W+QGEENI LDASTCAMAFRMLRV GY+VSSDQL QFSED F
Sbjct: 291 ARHFKEEIRSVLDETYRCWMQGEENIFLDASTCAMAFRMLRVEGYDVSSDQLTQFSEDIF 350

Query: 301 SDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRLHRN 360
            +CLGGYLKD G+ LEL+KASQI  +P++ VLENINSWTS FL +GLSS+S+ SDR    
Sbjct: 351 PNCLGGYLKDFGASLELYKASQIITHPDESVLENINSWTSRFLKHGLSSDSVWSDRTDSV 410

Query: 361 MKQEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMEDFN 420
           +KQE ++AL+F YN TLERL  K A+E YS + +RI K+PY+CLN GHQDFL LA+EDFN
Sbjct: 411 VKQEAVNALEFPYNATLERLISKRAMESYSGDIVRISKSPYACLNFGHQDFLELAVEDFN 470

Query: 421 VCQCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKNGV 480
             Q IH +E ++L+RWVVENKLD+L   R  L YCYF AAA +T PEL DAR++WA+NGV
Sbjct: 471 TLQRIHLKELEELQRWVVENKLDELKFFRLHLGYCYFAAAATLTDPELHDARIAWAQNGV 530

Query: 481 LTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKAL 540
           LTTVVDD +D  GSE+E  NL++ VE+WD   ++ YCS+ VEI+F ALHSTV EIG +AL
Sbjct: 531 LTTVVDDFYDGGGSEEELDNLIELVEKWDPDGEVGYCSKDVEIVFLALHSTVCEIGRRAL 590

Query: 541 TWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPALYF 600
            WQGR V  +V D WL L++ MR+EAEW +NK VPSM +YME  ++SFALGPI+LP L+F
Sbjct: 591 VWQGRSVMRNVIDGWLALLKVMRKEAEWSTNKVVPSMGEYMEQAHVSFALGPIILPMLFF 650

Query: 601 IGPKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNRTN 660
           +GPKLSEE++GS EY  L+KLMST GRL ND+++ +RE  EGKLN LSL MI+ GGN T 
Sbjct: 651 VGPKLSEEMIGSCEYQKLYKLMSTAGRLKNDIRSYDRECKEGKLNILSLWMIDGGGNVTK 710

Query: 661 EEVIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSLGL 720
           EE I AI+ D +++ RELL LVLQE ++TIPRACKDLFW++  +V+LFY +DDG+TS  L
Sbjct: 711 EEAIEAIKGDFERAIRELLGLVLQE-NTTIPRACKDLFWKLMSIVNLFYMEDDGYTSNRL 770

Query: 721 MNSAKALFEEPISFDNL 735
           MN+ KA+FE+P+  D L
Sbjct: 771 MNTVKAMFEQPMDLDAL 786

BLAST of Sgr028675 vs. ExPASy Swiss-Prot
Match: A0A1Z3GC64 (Ent-kaurene synthase 5, chloroplastic OS=Isodon rubescens OX=587669 GN=KSL5 PE=1 SV=1)

HSP 1 Score: 823.5 bits (2126), Expect = 1.8e-237
Identity = 403/734 (54.90%), Postives = 528/734 (71.93%), Query Frame = 0

Query: 1   MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
           +F K ELSVS YDTAW+A+VP+P   + P FP C+NWLL+NQ  DGSW   H H +L K 
Sbjct: 56  LFHKNELSVSTYDTAWVAMVPSPTSSEEPCFPACLNWLLENQCHDGSWARPHHHHMLKKD 115

Query: 61  TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
            L STLAC+L LK+W +G+E + +GL+F+E N  SAT++ Q +P+GFDIIFP M++ A+ 
Sbjct: 116 VLSSTLACILALKKWGVGEEQINRGLHFVELNFASATEKGQITPMGFDIIFPAMLDNARG 175

Query: 121 LELNLPLTQMNVDALIHRRELEL---NRSNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRK 180
           L LNL L    ++ LI++R+LEL   N+SNS  ++ Y A+++EG+GK QDW+ VM +QRK
Sbjct: 176 LSLNLQLEPTTLNDLIYKRDLELKRCNQSNSAEKEVYWAHIAEGMGKLQDWESVMKYQRK 235

Query: 181 NGSLFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLG 240
           NGSLFN PSTTAAAF  L N  CL+YL   L+KFG+AVP +YPLD Y+QLC VD+L+ LG
Sbjct: 236 NGSLFNCPSTTAAAFTALRNSDCLNYLRLALEKFGSAVPAVYPLDIYSQLCTVDNLERLG 295

Query: 241 IDHHFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDR 300
           I  +F  EI  VLD+TYR WLQG+E I +DASTCA+AFR LR+ GY V+SD + +  ++ 
Sbjct: 296 ISRYFLTEIQSVLDETYRSWLQGDEEIFMDASTCALAFRTLRMNGYNVTSDPITKILQEC 355

Query: 301 FSDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNS-IHSDRLH 360
           FS    G + DI + LE+++AS++ +YPE+  L+  N     FL   LSSN  I S +L 
Sbjct: 356 FSSSFRGNMTDINTTLEIYRASELILYPEERDLDQHNLRLKTFLEQELSSNGFIQSCQLG 415

Query: 361 RNMKQEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMED 420
           RN+  EV  A+++ +   ++R+  +  IE+Y+++N RILKT Y   N G++DFL L++ED
Sbjct: 416 RNINAEVNQAIEYPFYAIMDRMAKRKNIENYNIDNTRILKTSYRSPNFGNKDFLSLSVED 475

Query: 421 FNVCQCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKN 480
           FN CQ IHR+E ++LERWV+EN+LD+L  AR K  YCYF+AAA I SPELSDAR+SWAKN
Sbjct: 476 FNRCQVIHREELRELERWVIENRLDELKFARSKAAYCYFSAAATIFSPELSDARMSWAKN 535

Query: 481 GVLTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNK 540
           GVLTTVVDD FDV GS +E  NL+Q VE WDV    + CS  V+IIF AL  T+ EI +K
Sbjct: 536 GVLTTVVDDFFDVGGSVEELKNLIQLVELWDVDVSTQCCSPNVQIIFSALKHTICEIADK 595

Query: 541 ALTWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPAL 600
               QGR +T H+  IWL+L+ SM +E E   +K+ P+MD+YM N Y+SFALGPIVLPAL
Sbjct: 596 GFKLQGRSITDHIISIWLDLLYSMMKETELGIDKSFPTMDEYMSNAYVSFALGPIVLPAL 655

Query: 601 YFIGPKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNR 660
           Y +GPKLSEE+V   EYH LFKLMSTCGRLLND++  ERE  +GK++A+SL+++N GG  
Sbjct: 656 YLVGPKLSEEMVNHSEYHTLFKLMSTCGRLLNDIRGYERELKDGKISAVSLYIMNNGGEI 715

Query: 661 TNEEVIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSL 720
           T E  I  +   I++ RRELLRLVL+E  S +P+ACK LFW M  VVHLFYRKDDGFTSL
Sbjct: 716 TTEAAISEMRSWIERDRRELLRLVLEENKSVLPKACKKLFWHMCTVVHLFYRKDDGFTSL 775

Query: 721 GLMNSAKALFEEPI 730
            L     A+  EPI
Sbjct: 776 DLHGVVNAIINEPI 789

BLAST of Sgr028675 vs. ExPASy Swiss-Prot
Match: A0A2K9RFY0 (Ent-kaurene synthase TSP4, chloroplastic OS=Vitex agnus-castus OX=54477 GN=TPS4 PE=1 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 3.1e-237
Identity = 404/732 (55.19%), Postives = 532/732 (72.68%), Query Frame = 0

Query: 1   MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
           +  K ELSVS YDTAW+A+VP+PN  + P FP+C++WLL NQ  DGSW   H HP+L K 
Sbjct: 55  LIHKAELSVSTYDTAWVAMVPSPNSSQEPCFPDCLSWLLQNQCCDGSWACPHHHPLLKKD 114

Query: 61  TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
            L STLAC+L LK+W +G+E + +G++FIE N  SA ++ Q SP+GFDIIFP M++YA+ 
Sbjct: 115 VLCSTLACVLALKKWGVGEEKINRGVHFIEHNFASAMEKCQISPMGFDIIFPAMLDYARD 174

Query: 121 LELNLPLTQMNVDALIHRRELEL--NRSNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRKN 180
           L LNL L    ++ LI++R LEL  N+++S  R+AYLAYV+EG+GK QD   VM  QR+N
Sbjct: 175 LLLNLRLEPTMLNDLIYKRGLELKRNQNHSAEREAYLAYVAEGMGKLQDLGSVMKHQRRN 234

Query: 181 GSLFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLGI 240
           GSLFNSPSTTAAAF+   N  CL YL S L+KFG+AVP +YPLD Y QLC VD+L+ LGI
Sbjct: 235 GSLFNSPSTTAAAFIAFPNSRCLTYLRSALKKFGSAVPAVYPLDIYLQLCTVDNLERLGI 294

Query: 241 DHHFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDRF 300
             +F++EI  VLD+TYR WLQG E I +DA TCA+AFR+LR  GY V+SD + +  E+ F
Sbjct: 295 SRYFQKEIQGVLDETYRCWLQGNEEIFMDAPTCALAFRVLRKNGYNVTSDPITKLLEECF 354

Query: 301 SDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRLHRN 360
           S    G +KDI + L L++AS+  +YP++  LE  N    + L   LSS+ IHS +L RN
Sbjct: 355 SSSFCGNIKDINTTLGLYRASEFILYPDERDLEKQNLMLKNLLEQELSSDFIHSSQLGRN 414

Query: 361 MKQEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMEDFN 420
           +  EV  AL++ +   L+R+  +  IEHY+ +N RILKT Y   N G++DFL L+++D+N
Sbjct: 415 IDAEVKHALEYPFYADLDRIVNRRNIEHYNFDNTRILKTSYCSPNFGNKDFLFLSVKDYN 474

Query: 421 VCQCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKNGV 480
            CQ IHR+E ++LERWV+EN+LD+L  ARQK  YCYF+AAA + +PELS+AR+SWAKNGV
Sbjct: 475 ECQAIHREELRELERWVIENRLDELRFARQKCAYCYFSAAATLFAPELSNARMSWAKNGV 534

Query: 481 LTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKAL 540
           LTTVVDD FD+ GS +E  NL+Q VE WDV    E  S+ V+IIF AL  T+ +IG+K  
Sbjct: 535 LTTVVDDFFDLGGSVEELKNLIQLVELWDVDVSTECSSQNVQIIFSALKCTICDIGDKGS 594

Query: 541 TWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPALYF 600
             Q R +T+H+ DIWL+L+ SM +E EW  +K +P+MD+Y+ N Y+SFALGPIVLPALY 
Sbjct: 595 KLQERSITNHIIDIWLDLLYSMMKETEWARDKYIPTMDEYISNAYVSFALGPIVLPALYL 654

Query: 601 IGPKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNRTN 660
           +GPKLSEE+V   EYHNL+KLMSTCGRLLND++  ERE  EGKLNA+ L++IN GG  T 
Sbjct: 655 VGPKLSEEMVHHSEYHNLYKLMSTCGRLLNDIRGCERELKEGKLNAIPLYIINNGGEITK 714

Query: 661 EEVIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSLGL 720
           E     ++  I+  RRELLRLVL+ K+S +P++CK+LFW MS V+HLFY KDDGFTS  L
Sbjct: 715 EAAASEMKSLIETHRRELLRLVLEGKNSVLPKSCKELFWHMSKVLHLFYSKDDGFTSQDL 774

Query: 721 MNSAKALFEEPI 730
           +   KA+  EPI
Sbjct: 775 IKVVKAVIYEPI 786

BLAST of Sgr028675 vs. ExPASy Swiss-Prot
Match: A0A0U4CDK4 (Ent-kaur-16-ene synthase, chloroplastic OS=Salvia miltiorrhiza OX=226208 GN=KS PE=1 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 1.6e-236
Identity = 398/733 (54.30%), Postives = 522/733 (71.21%), Query Frame = 0

Query: 1   MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
           +F K ELSVS YDTAW+A+VP+P  L+ P FP+C+NWLL+NQ  DGSW   H HP+L K 
Sbjct: 59  LFHKDELSVSTYDTAWVAMVPSPTSLEEPCFPDCLNWLLENQCHDGSWARPHHHPLLKKD 118

Query: 61  TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
            L STLAC+L LK+W +G+E + +GL+F+E N  SATD+ Q +P+GFDIIFP M++YA+ 
Sbjct: 119 VLSSTLACILALKKWGVGEEQIKRGLHFLELNFASATDKCQITPMGFDIIFPAMLDYARG 178

Query: 121 LELNLPLTQMNVDALIHRRELELNRSN---SEGRKAYLAYVSEGIGKSQDWDMVMNFQRK 180
             LNL L     + L+H+R++EL RSN   S   + Y AY++EG+G+ Q+W+ VM +QR+
Sbjct: 179 FSLNLRLDPTTFNDLMHKRDVELKRSNRNYSSETETYWAYIAEGMGELQNWESVMKYQRR 238

Query: 181 NGSLFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLG 240
           NGSLFN PSTTAAAF+ L N  CL+YLH  L+KFGNAV  +YPLD Y+QLC VD+L+ LG
Sbjct: 239 NGSLFNCPSTTAAAFIALRNSDCLNYLHLALKKFGNAVSAVYPLDIYSQLCTVDNLERLG 298

Query: 241 IDHHFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDR 300
           I  +F  EI  VLD+TYR W+QG E I +DASTCA+AFR LR+ GY+V+SD + Q  ++ 
Sbjct: 299 ISQYFSTEIQNVLDETYRCWMQGNEEIFMDASTCALAFRTLRLNGYDVTSDPVTQILQEC 358

Query: 301 FSDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRLHR 360
           FS    G + DI + LEL++AS++ +YP++  LE  N      L   LSS  I S +L R
Sbjct: 359 FSSSFRGNMTDINTTLELYRASELVLYPDERDLEKQNLRLKLLLEQELSSGLIQSCQLGR 418

Query: 361 NMKQEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMEDF 420
           ++  EV  A+++ +   ++R+  + +IEHY+ +N RILKT Y   N  ++DFL L++EDF
Sbjct: 419 SINVEVNQAIEYPFYAIMDRVAKRKSIEHYNFDNTRILKTSYCSPNFSNEDFLFLSIEDF 478

Query: 421 NVCQCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKNG 480
           N CQ  HR+E  +LERWVVEN+LD+L  AR K  YCYF+AAA   +PEL DARLSWAKNG
Sbjct: 479 NRCQAAHREELGELERWVVENRLDELKFARSKSAYCYFSAAATFFAPELLDARLSWAKNG 538

Query: 481 VLTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKA 540
           VLTTV+DD FDV GS +E  NL+Q VE WDV    E  S  V+IIF AL  T+ EIG+KA
Sbjct: 539 VLTTVIDDFFDVGGSVEELKNLIQLVELWDVDISTECYSHNVQIIFSALRRTICEIGDKA 598

Query: 541 LTWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPALY 600
              QGRC+T+H+  IWL+L+ SM +E EW  +  VP++D+YM N ++SFALGPIVLPALY
Sbjct: 599 FKLQGRCITNHIIAIWLDLLNSMMRETEWARDNFVPTIDEYMSNAHVSFALGPIVLPALY 658

Query: 601 FIGPKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNRT 660
            +GPKLSEE+V   EYHNLFKLMSTCGRLLND+   ERE  +GKLNALSL++IN GG  +
Sbjct: 659 LVGPKLSEEMVNHSEYHNLFKLMSTCGRLLNDIHGYERELKDGKLNALSLYIINHGGEVS 718

Query: 661 NEEVIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSLG 720
            E  I  ++  I+  RRELLRLVL+ K S +P+ C++LFW M  VVHLFY K DGFTS  
Sbjct: 719 KEAAIWEMKSWIETQRRELLRLVLEGKKSVLPKPCRELFWHMCSVVHLFYSKGDGFTSQD 778

Query: 721 LMNSAKALFEEPI 730
           L+     +  +PI
Sbjct: 779 LIQLVNTIIHQPI 791

BLAST of Sgr028675 vs. ExPASy Swiss-Prot
Match: A0A3G1QTS7 (Ent-kaurene synthase 1, chloroplastic OS=Isodon eriocalyx OX=662907 GN=KS1 PE=1 SV=1)

HSP 1 Score: 809.7 bits (2090), Expect = 2.8e-233
Identity = 398/734 (54.22%), Postives = 523/734 (71.25%), Query Frame = 0

Query: 1   MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
           +F K ELSVS YDTAW+A+VP+P   + P FP C+NWLL+NQ  DGSW   H H +L K 
Sbjct: 56  LFHKNELSVSTYDTAWVAMVPSPTSSEEPCFPACLNWLLENQCHDGSWARPHHHHMLKKD 115

Query: 61  TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
            L STLAC+L LK+W +G+E + +GL+F+E N  SAT++ Q +P+GFDIIFP M++ A+ 
Sbjct: 116 VLSSTLACILALKKWGVGEEQINRGLHFVELNFASATEKGQITPMGFDIIFPAMLDNARG 175

Query: 121 LELNLPLTQMNVDALIHRRELEL---NRSNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRK 180
           L LNL L    ++ LI++R+LEL   N+SNS  ++ Y A+++EG+GK  DW+ VM +QRK
Sbjct: 176 LSLNLQLEPTMLNDLIYKRDLELKRCNQSNSAEKEVYWAHIAEGMGKLHDWESVMKYQRK 235

Query: 181 NGSLFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLG 240
           NGSLFN PSTTAAAF  L N  CL+YL   L+KFG+AVP +YPLD Y+QLC V +L+ LG
Sbjct: 236 NGSLFNCPSTTAAAFTALRNSDCLNYLCLALEKFGSAVPAVYPLDIYSQLCTVGNLERLG 295

Query: 241 IDHHFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDR 300
           I  +F  EI  VLD+TYR WLQG+E I +DASTCA+AFR LR+ GY V+SD + +  ++ 
Sbjct: 296 ISRYFLTEIQSVLDETYRSWLQGDEEIFMDASTCALAFRTLRMNGYNVTSDPITKILQEC 355

Query: 301 FSDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNS-IHSDRLH 360
           FS    G + D  + LE+++AS++ +YPE+  L   N     FL   LSSN  I S +L 
Sbjct: 356 FSSSFRGNMTDNNTTLEIYRASELILYPEERDLVQHNLRLKTFLEQELSSNGFIQSCQLG 415

Query: 361 RNMKQEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMED 420
           RN+  EV  A+++ +   ++R+  +  IE+Y+++N RILKT Y   N G++DFL L++ED
Sbjct: 416 RNINAEVNQAIEYPFYAIMDRMAKRKNIENYNIDNTRILKTSYRSPNFGNKDFLSLSVED 475

Query: 421 FNVCQCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKN 480
           FN CQ IHR+E ++LERWV+EN+LD+L  AR K  YCYF+AAA I SPELSDAR+SWAKN
Sbjct: 476 FNRCQVIHREELRELERWVIENRLDELKFARSKAAYCYFSAAATIFSPELSDARMSWAKN 535

Query: 481 GVLTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNK 540
           GVLTTVVDD FDV GS +E  NL+Q VE WDV    + CS  V+IIF AL  T+ EI +K
Sbjct: 536 GVLTTVVDDFFDVGGSVEELKNLIQLVELWDVDVSTQCCSPNVQIIFSALKHTICEIADK 595

Query: 541 ALTWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPAL 600
               QGR +T H+  IWL+L+ SM +E E   +K+ P+MD+YM N Y+SFALGPIVLPAL
Sbjct: 596 GFKLQGRSITDHIISIWLDLLYSMMKETELGIDKSFPTMDEYMSNAYVSFALGPIVLPAL 655

Query: 601 YFIGPKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNR 660
           Y +GPKLSEE+V   EYH LFKLMSTCGRLLND++  ERE  +GK++A+SL+++N GG  
Sbjct: 656 YLVGPKLSEEMVNHSEYHTLFKLMSTCGRLLNDIRGYERELKDGKISAVSLYIMNNGGEI 715

Query: 661 TNEEVIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSL 720
           T E  I  +   I++ RRELLRLVL+E  S +P+ACK LFW M  VVH+FY KDDGFTSL
Sbjct: 716 TTEAAISEMRSWIERDRRELLRLVLEENKSVLPKACKKLFWHMCTVVHVFYSKDDGFTSL 775

Query: 721 GLMNSAKALFEEPI 730
            L     A+  EPI
Sbjct: 776 NLHGVVNAIINEPI 789

BLAST of Sgr028675 vs. ExPASy TrEMBL
Match: A0A6J1D2F0 (ent-kaur-16-ene synthase, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016726 PE=4 SV=1)

HSP 1 Score: 1117.4 bits (2889), Expect = 0.0e+00
Identity = 539/735 (73.33%), Postives = 625/735 (85.03%), Query Frame = 0

Query: 1   MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
           MF KVELSVSAYDTAW+A+VP+PN L  PLFPECINW+LDNQ PDGSWG +H H +L KA
Sbjct: 96  MFNKVELSVSAYDTAWVAMVPSPNSLNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKA 155

Query: 61  TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
           +LLSTLAC L LKRW+IG EHM K LNFI+SN+ SATDEKQ SP+GFDIIFPGMIEYAK 
Sbjct: 156 SLLSTLACALTLKRWNIGHEHMSKALNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKD 215

Query: 121 LELNLPLTQMNVDALIHRRELELNRSNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRKNGS 180
           L+LNLPL   NVDAL+ ++EL+L RSNSEG KAYLAYVSEGIG+ +DW+MVM +QRKNGS
Sbjct: 216 LDLNLPLAPANVDALLQKKELDLRRSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKNGS 275

Query: 181 LFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLGIDH 240
           LFNSPSTTA AFM+ N+D C +YLHSLLQKF N+VPTIYPLD YA+LCMVD+LQ LGID 
Sbjct: 276 LFNSPSTTAVAFMHGNDDGCFNYLHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGIDR 335

Query: 241 HFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDRFSD 300
           HFKEEI  VLD+TYR W+QGEE+I LDASTCAMAFRMLR+ GY+VSSDQL QFSED FS+
Sbjct: 336 HFKEEIRSVLDETYRCWMQGEEDIFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLFSN 395

Query: 301 CLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRLHRNMK 360
           CLGGYLKD GS LELFKASQI IYP++ +LENINSWT+ FL++GLSS S+HSDR  R +K
Sbjct: 396 CLGGYLKDFGSSLELFKASQIIIYPDESILENINSWTNSFLSHGLSSGSVHSDRPDRLLK 455

Query: 361 QEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMEDFNVC 420
           QE I+AL+FTY+ TLERL  K A+E+Y+++ +RI KT Y+CLN GHQDFL LA+EDFN+ 
Sbjct: 456 QEAINALEFTYSNTLERLLNKRAMENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFNIL 515

Query: 421 QCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKNGVLT 480
           Q IH +E K+LERWVVEN+LDKL  ARQKL YCYF+AAA +TSPEL DARLSWAKNGVLT
Sbjct: 516 QSIHHKELKELERWVVENRLDKLKFARQKLAYCYFSAAATLTSPELRDARLSWAKNGVLT 575

Query: 481 TVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKALTW 540
           TVVDD FDV GSE E INL+Q VE+WDV  + +YCS++VEIIF ALH TV EI  +AL W
Sbjct: 576 TVVDDFFDVGGSEGELINLIQLVEKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRALPW 635

Query: 541 QGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPALYFIG 600
           QGR V  +V DIWL L++SMR+EAEWL NK  P MD+YMENGYISFALGPIVLPAL+F+G
Sbjct: 636 QGRSVMRNVIDIWLALLKSMRKEAEWLKNKIAPPMDEYMENGYISFALGPIVLPALFFVG 695

Query: 601 PKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNRTNEE 660
           PKL EEV+GS EYH LFKLMSTCGRLLND Q+ ERESSEGKLNA+SL MIN GG+ T EE
Sbjct: 696 PKLPEEVIGSCEYHKLFKLMSTCGRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTKEE 755

Query: 661 VIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSLGLMN 720
              AI+ +++++RRELLRLVLQEK+ST+PRACKDLFW+MSCVVHLFYRKDDGFTS  LMN
Sbjct: 756 ASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHELMN 815

Query: 721 SAKALFEEPISFDNL 735
           SAKAL E+P+  D L
Sbjct: 816 SAKALLEQPMVLDEL 830

BLAST of Sgr028675 vs. ExPASy TrEMBL
Match: A0A6J1D2L1 (ent-kaur-16-ene synthase, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016726 PE=4 SV=1)

HSP 1 Score: 1112.4 bits (2876), Expect = 0.0e+00
Identity = 539/737 (73.13%), Postives = 625/737 (84.80%), Query Frame = 0

Query: 1   MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
           MF KVELSVSAYDTAW+A+VP+PN L  PLFPECINW+LDNQ PDGSWG +H H +L KA
Sbjct: 96  MFNKVELSVSAYDTAWVAMVPSPNSLNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKA 155

Query: 61  TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
           +LLSTLAC L LKRW+IG EHM K LNFI+SN+ SATDEKQ SP+GFDIIFPGMIEYAK 
Sbjct: 156 SLLSTLACALTLKRWNIGHEHMSKALNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKD 215

Query: 121 LELNLPLTQMNVDALIHRRELELN--RSNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRKN 180
           L+LNLPL   NVDAL+ ++EL+L   RSNSEG KAYLAYVSEGIG+ +DW+MVM +QRKN
Sbjct: 216 LDLNLPLAPANVDALLQKKELDLRSCRSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKN 275

Query: 181 GSLFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLGI 240
           GSLFNSPSTTA AFM+ N+D C +YLHSLLQKF N+VPTIYPLD YA+LCMVD+LQ LGI
Sbjct: 276 GSLFNSPSTTAVAFMHGNDDGCFNYLHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGI 335

Query: 241 DHHFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDRF 300
           D HFKEEI  VLD+TYR W+QGEE+I LDASTCAMAFRMLR+ GY+VSSDQL QFSED F
Sbjct: 336 DRHFKEEIRSVLDETYRCWMQGEEDIFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLF 395

Query: 301 SDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRLHRN 360
           S+CLGGYLKD GS LELFKASQI IYP++ +LENINSWT+ FL++GLSS S+HSDR  R 
Sbjct: 396 SNCLGGYLKDFGSSLELFKASQIIIYPDESILENINSWTNSFLSHGLSSGSVHSDRPDRL 455

Query: 361 MKQEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMEDFN 420
           +KQE I+AL+FTY+ TLERL  K A+E+Y+++ +RI KT Y+CLN GHQDFL LA+EDFN
Sbjct: 456 LKQEAINALEFTYSNTLERLLNKRAMENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFN 515

Query: 421 VCQCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKNGV 480
           + Q IH +E K+LERWVVEN+LDKL  ARQKL YCYF+AAA +TSPEL DARLSWAKNGV
Sbjct: 516 ILQSIHHKELKELERWVVENRLDKLKFARQKLAYCYFSAAATLTSPELRDARLSWAKNGV 575

Query: 481 LTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKAL 540
           LTTVVDD FDV GSE E INL+Q VE+WDV  + +YCS++VEIIF ALH TV EI  +AL
Sbjct: 576 LTTVVDDFFDVGGSEGELINLIQLVEKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRAL 635

Query: 541 TWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPALYF 600
            WQGR V  +V DIWL L++SMR+EAEWL NK  P MD+YMENGYISFALGPIVLPAL+F
Sbjct: 636 PWQGRSVMRNVIDIWLALLKSMRKEAEWLKNKIAPPMDEYMENGYISFALGPIVLPALFF 695

Query: 601 IGPKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNRTN 660
           +GPKL EEV+GS EYH LFKLMSTCGRLLND Q+ ERESSEGKLNA+SL MIN GG+ T 
Sbjct: 696 VGPKLPEEVIGSCEYHKLFKLMSTCGRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTK 755

Query: 661 EEVIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSLGL 720
           EE   AI+ +++++RRELLRLVLQEK+ST+PRACKDLFW+MSCVVHLFYRKDDGFTS  L
Sbjct: 756 EEASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHEL 815

Query: 721 MNSAKALFEEPISFDNL 735
           MNSAKAL E+P+  D L
Sbjct: 816 MNSAKALLEQPMVLDEL 832

BLAST of Sgr028675 vs. ExPASy TrEMBL
Match: A0A6J1D1G1 (ent-kaur-16-ene synthase, chloroplastic isoform X3 OS=Momordica charantia OX=3673 GN=LOC111016726 PE=4 SV=1)

HSP 1 Score: 1112.4 bits (2876), Expect = 0.0e+00
Identity = 539/737 (73.13%), Postives = 625/737 (84.80%), Query Frame = 0

Query: 1   MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
           MF KVELSVSAYDTAW+A+VP+PN L  PLFPECINW+LDNQ PDGSWG +H H +L KA
Sbjct: 83  MFNKVELSVSAYDTAWVAMVPSPNSLNDPLFPECINWVLDNQHPDGSWGIIHNHHLLMKA 142

Query: 61  TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
           +LLSTLAC L LKRW+IG EHM K LNFI+SN+ SATDEKQ SP+GFDIIFPGMIEYAK 
Sbjct: 143 SLLSTLACALTLKRWNIGHEHMSKALNFIKSNIVSATDEKQYSPVGFDIIFPGMIEYAKD 202

Query: 121 LELNLPLTQMNVDALIHRRELELN--RSNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRKN 180
           L+LNLPL   NVDAL+ ++EL+L   RSNSEG KAYLAYVSEGIG+ +DW+MVM +QRKN
Sbjct: 203 LDLNLPLAPANVDALLQKKELDLRSCRSNSEGGKAYLAYVSEGIGRLRDWEMVMQYQRKN 262

Query: 181 GSLFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLGI 240
           GSLFNSPSTTA AFM+ N+D C +YLHSLLQKF N+VPTIYPLD YA+LCMVD+LQ LGI
Sbjct: 263 GSLFNSPSTTAVAFMHGNDDGCFNYLHSLLQKFDNSVPTIYPLDIYARLCMVDNLQRLGI 322

Query: 241 DHHFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDRF 300
           D HFKEEI  VLD+TYR W+QGEE+I LDASTCAMAFRMLR+ GY+VSSDQL QFSED F
Sbjct: 323 DRHFKEEIRSVLDETYRCWMQGEEDIFLDASTCAMAFRMLRLGGYDVSSDQLTQFSEDLF 382

Query: 301 SDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRLHRN 360
           S+CLGGYLKD GS LELFKASQI IYP++ +LENINSWT+ FL++GLSS S+HSDR  R 
Sbjct: 383 SNCLGGYLKDFGSSLELFKASQIIIYPDESILENINSWTNSFLSHGLSSGSVHSDRPDRL 442

Query: 361 MKQEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMEDFN 420
           +KQE I+AL+FTY+ TLERL  K A+E+Y+++ +RI KT Y+CLN GHQDFL LA+EDFN
Sbjct: 443 LKQEAINALEFTYSNTLERLLNKRAMENYNIDIVRISKTTYTCLNFGHQDFLELAVEDFN 502

Query: 421 VCQCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKNGV 480
           + Q IH +E K+LERWVVEN+LDKL  ARQKL YCYF+AAA +TSPEL DARLSWAKNGV
Sbjct: 503 ILQSIHHKELKELERWVVENRLDKLKFARQKLAYCYFSAAATLTSPELRDARLSWAKNGV 562

Query: 481 LTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKAL 540
           LTTVVDD FDV GSE E INL+Q VE+WDV  + +YCS++VEIIF ALH TV EI  +AL
Sbjct: 563 LTTVVDDFFDVGGSEGELINLIQLVEKWDVNGEADYCSKEVEIIFLALHGTVCEIEKRAL 622

Query: 541 TWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPALYF 600
            WQGR V  +V DIWL L++SMR+EAEWL NK  P MD+YMENGYISFALGPIVLPAL+F
Sbjct: 623 PWQGRSVMRNVIDIWLALLKSMRKEAEWLKNKIAPPMDEYMENGYISFALGPIVLPALFF 682

Query: 601 IGPKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNRTN 660
           +GPKL EEV+GS EYH LFKLMSTCGRLLND Q+ ERESSEGKLNA+SL MIN GG+ T 
Sbjct: 683 VGPKLPEEVIGSCEYHKLFKLMSTCGRLLNDTQSFERESSEGKLNAMSLCMINAGGDFTK 742

Query: 661 EEVIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSLGL 720
           EE   AI+ +++++RRELLRLVLQEK+ST+PRACKDLFW+MSCVVHLFYRKDDGFTS  L
Sbjct: 743 EEASEAIKGNVERTRRELLRLVLQEKNSTVPRACKDLFWKMSCVVHLFYRKDDGFTSHEL 802

Query: 721 MNSAKALFEEPISFDNL 735
           MNSAKAL E+P+  D L
Sbjct: 803 MNSAKALLEQPMVLDEL 819

BLAST of Sgr028675 vs. ExPASy TrEMBL
Match: A0A1S4DWJ8 (ent-kaur-16-ene synthase, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103489622 PE=4 SV=1)

HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 530/735 (72.11%), Postives = 618/735 (84.08%), Query Frame = 0

Query: 1   MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
           +F+ VELSVSAYDTAW+A+VP+PN L  PLFPECINW+LD+Q PDGSWG LH H ++ KA
Sbjct: 47  LFKNVELSVSAYDTAWVAMVPSPNSLNKPLFPECINWVLDHQNPDGSWGILHDHQLVMKA 106

Query: 61  TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
           TLLSTLAC+L LKRW+IG +HM K L+FI+SN+ SATDE QRSP+GFDIIFPGMIEYAK 
Sbjct: 107 TLLSTLACVLTLKRWNIGHDHMSKALSFIKSNIASATDENQRSPVGFDIIFPGMIEYAKD 166

Query: 121 LELNLPLTQMNVDALIHRRELELNRSNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRKNGS 180
           L LNLPL  MNVDAL+ ++ELEL RSNSEG KAYLAYVSEGIGK QDWDMVM +QRKNGS
Sbjct: 167 LNLNLPLASMNVDALVQKKELEL-RSNSEGGKAYLAYVSEGIGKLQDWDMVMQYQRKNGS 226

Query: 181 LFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLGIDH 240
           LF+SPSTTA AFM+ N+D C +YL S+LQKF ++VP IYPLD YA+L MVDSLQ LGID 
Sbjct: 227 LFSSPSTTAVAFMHRNDDGCFNYLRSILQKFHSSVPAIYPLDIYARLHMVDSLQKLGIDR 286

Query: 241 HFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDRFSD 300
           HFK+EI  VLD+TYR W+QGEENI LDASTCAMAFRMLRV GY+VSSDQL QFSED F +
Sbjct: 287 HFKDEIRSVLDETYRCWMQGEENIFLDASTCAMAFRMLRVEGYDVSSDQLTQFSEDLFPN 346

Query: 301 CLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRLHRNMK 360
           CLGG+LKD G+ LELFKASQI IYP++ +LENINSWTS FLN+GLS  S+HSDR  R +K
Sbjct: 347 CLGGHLKDFGASLELFKASQIIIYPDEFILENINSWTSRFLNHGLSGGSVHSDRTERLVK 406

Query: 361 QEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMEDFNVC 420
           QE ++A +F YN+TLERL  K A+E YS + +RI KT Y+CLN GHQDFL LA+EDFN  
Sbjct: 407 QEAVNAFEFPYNSTLERLSNKRALESYSGDIVRISKTTYACLNFGHQDFLELAVEDFNTL 466

Query: 421 QCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKNGVLT 480
           QCIHR+E K+LE+WV+ENKLDKL  ARQKL YCYF+AAA +TSPEL DARLSWAKNGVLT
Sbjct: 467 QCIHRKELKELEKWVIENKLDKLKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGVLT 526

Query: 481 TVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKALTW 540
           TVVDD FDV GSE+E +NL+Q VE+WD   +  YCS++VEIIF ALHST+ EIG KAL W
Sbjct: 527 TVVDDFFDVGGSEEELVNLIQLVEKWDASGEAGYCSKEVEIIFLALHSTICEIGEKALPW 586

Query: 541 QGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPALYFIG 600
           QGR VT +V DIWL L+ SMR+EAEWL NK VPS+D+YMENGY+SFALGPIVLP LYF+G
Sbjct: 587 QGRSVTRNVIDIWLALLESMRKEAEWLKNKVVPSLDEYMENGYVSFALGPIVLPTLYFVG 646

Query: 601 PKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNRTNEE 660
           PKL EE++G+ EY  LFKLMST GRLLND +T +RESSEGKLNALSL++I+ GG  + EE
Sbjct: 647 PKLPEEIIGNCEYQKLFKLMSTSGRLLNDTRTFDRESSEGKLNALSLYLISAGGKLSKEE 706

Query: 661 VIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSLGLMN 720
              A++ D+D++RRELLRLVLQE +STIPRACKDLFW+MSCVVHLFYRKDDGFTS  LMN
Sbjct: 707 ATEAMKGDVDRTRRELLRLVLQE-NSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHELMN 766

Query: 721 SAKALFEEPISFDNL 735
           SAKALFE P+  D L
Sbjct: 767 SAKALFELPMVLDEL 779

BLAST of Sgr028675 vs. ExPASy TrEMBL
Match: A0A1S3BG06 (ent-kaur-16-ene synthase, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103489622 PE=4 SV=1)

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 529/737 (71.78%), Postives = 617/737 (83.72%), Query Frame = 0

Query: 1   MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKA 60
           +F+ VELSVSAYDTAW+A+VP+PN L  PLFPECINW+LD+Q PDGSWG LH H ++ KA
Sbjct: 47  LFKNVELSVSAYDTAWVAMVPSPNSLNKPLFPECINWVLDHQNPDGSWGILHDHQLVMKA 106

Query: 61  TLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAKT 120
           TLLSTLAC+L LKRW+IG +HM K L+FI+SN+ SATDE QRSP+GFDIIFPGMIEYAK 
Sbjct: 107 TLLSTLACVLTLKRWNIGHDHMSKALSFIKSNIASATDENQRSPVGFDIIFPGMIEYAKD 166

Query: 121 LELNLPLTQMNVDALIHRRELELNR--SNSEGRKAYLAYVSEGIGKSQDWDMVMNFQRKN 180
           L LNLPL  MNVDAL+ ++ELEL    SNSEG KAYLAYVSEGIGK QDWDMVM +QRKN
Sbjct: 167 LNLNLPLASMNVDALVQKKELELRSCCSNSEGGKAYLAYVSEGIGKLQDWDMVMQYQRKN 226

Query: 181 GSLFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLGI 240
           GSLF+SPSTTA AFM+ N+D C +YL S+LQKF ++VP IYPLD YA+L MVDSLQ LGI
Sbjct: 227 GSLFSSPSTTAVAFMHRNDDGCFNYLRSILQKFHSSVPAIYPLDIYARLHMVDSLQKLGI 286

Query: 241 DHHFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSEDRF 300
           D HFK+EI  VLD+TYR W+QGEENI LDASTCAMAFRMLRV GY+VSSDQL QFSED F
Sbjct: 287 DRHFKDEIRSVLDETYRCWMQGEENIFLDASTCAMAFRMLRVEGYDVSSDQLTQFSEDLF 346

Query: 301 SDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRLHRN 360
            +CLGG+LKD G+ LELFKASQI IYP++ +LENINSWTS FLN+GLS  S+HSDR  R 
Sbjct: 347 PNCLGGHLKDFGASLELFKASQIIIYPDEFILENINSWTSRFLNHGLSGGSVHSDRTERL 406

Query: 361 MKQEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNLGHQDFLRLAMEDFN 420
           +KQE ++A +F YN+TLERL  K A+E YS + +RI KT Y+CLN GHQDFL LA+EDFN
Sbjct: 407 VKQEAVNAFEFPYNSTLERLSNKRALESYSGDIVRISKTTYACLNFGHQDFLELAVEDFN 466

Query: 421 VCQCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWAKNGV 480
             QCIHR+E K+LE+WV+ENKLDKL  ARQKL YCYF+AAA +TSPEL DARLSWAKNGV
Sbjct: 467 TLQCIHRKELKELEKWVIENKLDKLKFARQKLAYCYFSAAATLTSPELCDARLSWAKNGV 526

Query: 481 LTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKAL 540
           LTTVVDD FDV GSE+E +NL+Q VE+WD   +  YCS++VEIIF ALHST+ EIG KAL
Sbjct: 527 LTTVVDDFFDVGGSEEELVNLIQLVEKWDASGEAGYCSKEVEIIFLALHSTICEIGEKAL 586

Query: 541 TWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLPALYF 600
            WQGR VT +V DIWL L+ SMR+EAEWL NK VPS+D+YMENGY+SFALGPIVLP LYF
Sbjct: 587 PWQGRSVTRNVIDIWLALLESMRKEAEWLKNKVVPSLDEYMENGYVSFALGPIVLPTLYF 646

Query: 601 IGPKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGGNRTN 660
           +GPKL EE++G+ EY  LFKLMST GRLLND +T +RESSEGKLNALSL++I+ GG  + 
Sbjct: 647 VGPKLPEEIIGNCEYQKLFKLMSTSGRLLNDTRTFDRESSEGKLNALSLYLISAGGKLSK 706

Query: 661 EEVIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFTSLGL 720
           EE   A++ D+D++RRELLRLVLQE +STIPRACKDLFW+MSCVVHLFYRKDDGFTS  L
Sbjct: 707 EEATEAMKGDVDRTRRELLRLVLQE-NSTIPRACKDLFWKMSCVVHLFYRKDDGFTSHEL 766

Query: 721 MNSAKALFEEPISFDNL 735
           MNSAKALFE P+  D L
Sbjct: 767 MNSAKALFELPMVLDEL 782

BLAST of Sgr028675 vs. TAIR 10
Match: AT1G79460.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 790.4 bits (2040), Expect = 1.2e-228
Identity = 404/737 (54.82%), Postives = 526/737 (71.37%), Query Frame = 0

Query: 1   MFEKVELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGF-LHPHPILTK 60
           M EKVELSVSAYDT+W+A+VP+P+    PLFP+C+ WLLDNQ  DGSWG   H H  L K
Sbjct: 45  MLEKVELSVSAYDTSWVAMVPSPSSQNAPLFPQCVKWLLDNQHEDGSWGLDNHDHQSLKK 104

Query: 61  ATLLSTLACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEKQRSPIGFDIIFPGMIEYAK 120
             L STLA +L LK+W IG+  + KGL FIE N    TDE  + P GFDIIFPGMI+YA+
Sbjct: 105 DVLSSTLASILALKKWGIGERQINKGLQFIELNSALVTDETIQKPTGFDIIFPGMIKYAR 164

Query: 121 TLELNLPLTQMNVDALIHRRELEL---NRSNSEGRKAYLAYVSEGIGKSQDWDMVMNFQR 180
            L L +PL    VD +I +R+L+L   +   S+GR+AYLAYV EG    +DWD+++ +QR
Sbjct: 165 DLNLTIPLGSEVVDDMIRKRDLDLKCDSEKFSKGREAYLAYVLEGTRNLKDWDLIVKYQR 224

Query: 181 KNGSLFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTL 240
           KNGSLF+SP+TTAAAF    ND CL YL SLLQKF  AVP++YP D YA+L ++ +L++L
Sbjct: 225 KNGSLFDSPATTAAAFTQFGNDGCLRYLCSLLQKFEAAVPSVYPFDQYARLSIIVTLESL 284

Query: 241 GIDHHFKEEIGRVLDKTYRYWLQGEENILLDASTCAMAFRMLRVYGYEVSSDQLAQFSED 300
           GID  FK EI  +LD+TYRYWL+G+E I LD +TCA+AFR+L  +GY+VS D L  F+E+
Sbjct: 285 GIDRDFKTEIKSILDETYRYWLRGDEEICLDLATCALAFRLLLAHGYDVSYDPLKPFAEE 344

Query: 301 R-FSDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYGLSSNSIHSDRL 360
             FSD L GY+K+  SVLELFKA+Q   YP +  L+    WT  +L   LSS  + +   
Sbjct: 345 SGFSDTLEGYVKNTFSVLELFKAAQ--SYPHESALKKQCCWTKQYLEMELSS-WVKTSVR 404

Query: 361 HRNMKQEVISALDFTYNTTLERLQYKSAIEHYS-VNNIRILKTPYSCLNLGHQDFLRLAM 420
            + +K+EV  AL F    +LER  ++  I + S V N R+ KT Y   N+   D L+LA+
Sbjct: 405 DKYLKKEVEDALAFPSYASLERSDHRRKILNGSAVENTRVTKTSYRLHNICTSDILKLAV 464

Query: 421 EDFNVCQCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSWA 480
           +DFN CQ IHR+E + L+RW+VEN+L +L  ARQKL YCYF+ AA + SPELSDAR+SWA
Sbjct: 465 DDFNFCQSIHREEMERLDRWIVENRLQELKFARQKLAYCYFSGAATLFSPELSDARISWA 524

Query: 481 KNGVLTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIFKALHSTVDEIG 540
           K GVLTTVVDD FDV GS++E  NL+  VE+WD+    EY SE VEIIF  L  T+ E G
Sbjct: 525 KGGVLTTVVDDFFDVGGSKEELENLIHLVEKWDLNGVPEYSSEHVEIIFSVLRDTILETG 584

Query: 541 NKALTWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGYISFALGPIVLP 600
           +KA T+QGR VT H+  IWL+L++SM +EAEW S+K+ PS++DYMEN YISFALGPIVLP
Sbjct: 585 DKAFTYQGRNVTHHIVKIWLDLLKSMLREAEWSSDKSTPSLEDYMENAYISFALGPIVLP 644

Query: 601 ALYFIGPKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERESSEGKLNALSLHMINTGG 660
           A Y IGP L E+ V S +Y+ L+KL+ST GRLLND+Q  +RES+EGKLNA+SLHM +   
Sbjct: 645 ATYLIGPPLPEKTVDSHQYNQLYKLVSTMGRLLNDIQGFKRESAEGKLNAVSLHMKHERD 704

Query: 661 NRTNEEVIRAIEVDIDKSRRELLRLVLQEKDSTIPRACKDLFWRMSCVVHLFYRKDDGFT 720
           NR+ E +I +++   ++ R EL +LVL+EK S +PR CK+ F +MS V++LFYRKDDGFT
Sbjct: 705 NRSKEVIIESMKGLAERKREELHKLVLEEKGSVVPRECKEAFLKMSKVLNLFYRKDDGFT 764

Query: 721 SLGLMNSAKALFEEPIS 731
           S  LM+  K++  EP+S
Sbjct: 765 SNDLMSLVKSVIYEPVS 778

BLAST of Sgr028675 vs. TAIR 10
Match: AT1G61120.1 (terpene synthase 04 )

HSP 1 Score: 288.9 bits (738), Expect = 1.2e-77
Identity = 224/769 (29.13%), Postives = 370/769 (48.11%), Query Frame = 0

Query: 7   LSVSAYDTAWMALVP-----TPNCLKTPLFPECINWLLDNQ-LPDGSWGFLHPHPILT-- 66
           +S SAYDTAW++++        N LK P+F  C++W++ NQ   +G W     +  +   
Sbjct: 36  VSPSAYDTAWLSMIEEDINVDDNELK-PMFQGCLDWIMCNQNAREGFWMNSTSYTTVADG 95

Query: 67  --------KATLLSTLACLLPLKRWDIGQEHMIKGLNFIESN----LDSATDEKQRSPIG 126
                      L STLAC++ L++W+IG  H+ KG  +IE N    +    +E+   P  
Sbjct: 96  RDEDGEKDMCILTSTLACVVALQKWNIGCFHLHKGTRYIERNTEMIIGKYINEEGSYPRW 155

Query: 127 FDIIFPGMIEYAKTLELNLPLTQMNVDAL---------IHRRELELNRSNSEGRKAYLAY 186
           F I F G++E A+ L L+   +   ++ +         I +RE  ++  N +   AYL  
Sbjct: 156 FAIKFTGILELAQKLGLHFVFSSRCIEMIKGMFYQRQEIIQREKLVHDCNYKPLLAYLEV 215

Query: 187 VSEGIGKSQDWDMVM-NFQRKNGSLFNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVP 246
           +   +  +   D+++ +    +GSLF SPS TA+AFM   N  CL YL +L+QK  N VP
Sbjct: 216 LPSKLYVTNQEDIIVKSLDSMDGSLFQSPSATASAFMLTRNTKCLAYLQNLVQKCPNGVP 275

Query: 247 TIYPL-DTYAQLCMVDSLQTLGIDHHFKEEIGRVLDKTY-RYWLQGEENILL-----DAS 306
             YPL +   +L MV+ +++ G+   F  EI  VL++ Y RY  +  E + +        
Sbjct: 276 QKYPLNEDLIKLSMVNLIESTGLGEFFGIEIEHVLEQVYSRYEEKDFERMPMSYLADQLH 335

Query: 307 TCAMAFRMLRVYGYEVSSDQLAQFSEDRFSDCLGGYLKDIGS----VLELFKASQITIYP 366
             ++AFRMLR++G +VS      F  D+  +      ++I S    +L +++A+ + ++P
Sbjct: 336 KDSLAFRMLRMHGRDVSPRSFCWFLNDQ--ETRNHLERNIDSFLLVILSVYRATDL-MFP 395

Query: 367 EDPVLENINSWTSHFLNYGLSSNSIHSDRLHRNMKQEVI-SALDFTYNTTLERLQYKSAI 426
            +  L+    +T + L               R++K+++I   L   +   L+ L ++  I
Sbjct: 396 GEHDLQEAREYTRNLL------------EKRRSIKEKMIMHELSTPWIARLKHLDHRMWI 455

Query: 427 EHYSVNNIRILKTPYSCLNLGHQDFL-RLAMEDFNVCQCIHRQEKKDLERWVVENKLDKL 486
           E  + N + + K  +  L+  + D L  LA  +F   Q  + +E ++L  WV +  L  +
Sbjct: 456 EDKNSNVLSMEKASFLRLHSSYSDKLTHLAARNFEFQQAKYCRELEELTMWVKKWGLSDI 515

Query: 487 -IARQKLVYCYFTAAAAITSPELSDARLSWAKNGVLTTVVDDLFDVWGSEDERINLVQAV 546
              R+K  YCYF    ++            AK  +L T+ DD FD  GS ++   L +AV
Sbjct: 516 GFGREKTTYCYFATVTSLPYEYAIKFGKLAAKTAILITIADDFFDEKGSFNDLEGLTKAV 575

Query: 547 ERWDVCNDMEYCSEKVEIIFKALHSTVDEIGNKALTWQGRCVTSHVADIWLELIRSMRQE 606
            RW    + E       IIF+AL   V E  N   T     +  H+ +IW E   S  +E
Sbjct: 576 LRW----EGEELKSYGNIIFRALDDIVRETANTCRTHHKTDIIVHLRNIWGETFESWLRE 635

Query: 607 AEWLSNKAVPSMDDYMENGYISFALGPIVLPALYFIGPKLSEEVVGSFEYHNLFKLMSTC 666
           AEW       SMD+Y+ NG IS A   I L     + P      +    Y ++  L+   
Sbjct: 636 AEWSKKGHTSSMDEYIRNGMISIAAHTIALSISCLMEPCFPHNKLKPGNYDSITTLLMII 695

Query: 667 GRLLNDVQTIERESSEGKLNALSLHMINTGGNRTNEEVIRAIEVDIDKSRRELLRLVLQE 726
            RLLND+Q+ ++E  +GK+N++ LHM N  G    E+ I  IE  ID  R+E L  VL +
Sbjct: 696 PRLLNDLQSYQKEQEQGKMNSVLLHMKNHPGLEI-EDSIAHIEKIIDSKRKEFLEHVLVD 755

Query: 727 KDSTIPRACKDLFWRMSCVVHLFYRKDDGFTS-LGLMNSAKALFEEPIS 731
             S +P+ CK++      V  +F+ K + + S   +++  K    +PI+
Sbjct: 756 GLSDLPKPCKEIHMSCCKVFEMFFNKKNRYDSNTEMLHDIKKALYDPIN 783

BLAST of Sgr028675 vs. TAIR 10
Match: AT4G02780.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 283.5 bits (724), Expect = 4.9e-76
Identity = 160/494 (32.39%), Postives = 272/494 (55.06%), Query Frame = 0

Query: 6   ELSVSAYDTAWMALVPTPNCLKTPLFPECINWLLDNQLPDGSWGFLHPHPILTKATLLST 65
           E+++SAYDTAW+AL+   +  KTP FP  + W+ +NQL DGSWG  +      +  L++T
Sbjct: 112 EITISAYDTAWVALIDAGD--KTPAFPSAVKWIAENQLSDGSWGDAYLFSYHDR--LINT 171

Query: 66  LACLLPLKRWDIGQEHMIKGLNFIESNLDSATDEK-QRSPIGFDIIFPGMIEYAKTLELN 125
           LAC++ L+ W++      KG+ F   N+    DE  +  PIGF++ FP ++E A+ + ++
Sbjct: 172 LACVVALRSWNLFPHQCNKGITFFRENIGKLEDENDEHMPIGFEVAFPSLLEIARGINID 231

Query: 126 LPLTQMNVDALIHRRELELNRSNSE---GRKAYLAYVSEGIGKSQDWDMVMNFQRKNGSL 185
           +P     +  +  ++EL+L R   E        L +  EG+ +  DW+ ++  Q ++GS 
Sbjct: 232 VPYDSPVLKDIYAKKELKLTRIPKEIMHKIPTTLLHSLEGM-RDLDWEKLLKLQSQDGSF 291

Query: 186 FNSPSTTAAAFMNLNNDSCLDYLHSLLQKFGNAVPTIYPLDTYAQLCMVDSLQTLGIDHH 245
             SPS+TA AFM   + +CL+YL + +++F   VP ++P+D +  + +VD LQ LGI  +
Sbjct: 292 LFSPSSTAFAFMQTRDSNCLEYLRNAVKRFNGGVPNVFPVDLFEHIWIVDRLQRLGISRY 351

Query: 246 FKEEIGRVLDKTYRYWLQ-----GEENILLDASTCAMAFRMLRVYGYEVSSDQLAQF-SE 305
           F+EEI   LD  +RYW          + + D    AMAFR+LR +GY+VS+D    F  E
Sbjct: 352 FEEEIKECLDYVHRYWTDNGICWARCSHVQDIDDTAMAFRLLRQHGYQVSADVFKNFEKE 411

Query: 306 DRFSDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWT-SHFLNYGLSSNSIHSDR 365
             F   +G   + +  +  L++ASQ+  +P + +L+N   ++ ++ L        I    
Sbjct: 412 GEFFCFVGQSNQAVTGMFNLYRASQLA-FPREEILKNAKEFSYNYLLEKREREELIDKWI 471

Query: 366 LHRNMKQEVISALDFTYNTTLERLQYKSAIEHY-SVNNIRILKTPYSCLNLGHQDFLRLA 425
           + +++  E+  AL+  +  +L R++ +  I+ Y   N++ I KT Y    + +  +L LA
Sbjct: 472 IMKDLPGEIGFALEIPWYASLPRVETRFYIDQYGGENDVWIGKTLYRMPYVNNNGYLELA 531

Query: 426 MEDFNVCQCIHRQEKKDLERWVVENKLDKL-IARQKLVYCYFTAAAAITSPELSDARLSW 485
            +D+N CQ  H+ E    ++W  EN+L +  + R +L+ CY+ AAA I   E S  R+ W
Sbjct: 532 KQDYNNCQAQHQLEWDIFQKWYEENRLSEWGVRRSELLECYYLAAATIFESERSHERMVW 591

Query: 486 AKNGVLTTVVDDLF 487
           AK+ VL   +   F
Sbjct: 592 AKSSVLVKAISSSF 599

BLAST of Sgr028675 vs. TAIR 10
Match: AT4G16740.1 (terpene synthase 03 )

HSP 1 Score: 164.5 bits (415), Expect = 3.3e-40
Identity = 120/440 (27.27%), Postives = 220/440 (50.00%), Query Frame = 0

Query: 226 QLCMVDSLQTLGIDHHFKEEIGRVLDKTYRYWLQGEENIL-----LDASTCAMAFRMLRV 285
           QL ++D+LQ LG+ +HF++EI + L   +   ++  +N +      D    A+ FR+LR 
Sbjct: 77  QLELIDTLQRLGVSYHFEQEIKKTLTNVHVKNVRAHKNRIDRNRWGDLYATALEFRLLRQ 136

Query: 286 YGYEVSSDQLAQFSEDRFSDCLGGYL--KDIGSVLELFKASQITIYPEDPVLENINSWTS 345
           +G+ +        ++D F   +G  L  KDI  +L L++AS ++   +  + E+I   T 
Sbjct: 137 HGFSI--------AQDVFDGNIGVDLDDKDIKGILSLYEASYLSTRIDTKLKESIYYTTK 196

Query: 346 HFLNY-GLSSNSIHSDRLHRNMKQEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKT 405
               +  ++ N   S  L R     VI AL+  Y+  + RL+ +  IE Y   +      
Sbjct: 197 RLRKFVEVNKNETKSYTLRR----MVIHALEMPYHRRVGRLEARWYIEVYGERHDM---- 256

Query: 406 PYSCLNLGHQDFLRLAMEDFNVCQCIHRQEKKDLERWVVENKLDKLI--ARQKLVYCYFT 465
                   +   L LA  DFN  Q IH+ E K L  W  +  L K +   R ++   YF+
Sbjct: 257 --------NPILLELAKLDFNFVQAIHQDELKSLSSWWSKTGLTKHLDFVRDRITEGYFS 316

Query: 466 AAAAITSPELSDARLSWAKNGVLTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCS 525
           +   +  PE +  R    K  +L T +DD++D++G+ +E       VE+WDV N +E   
Sbjct: 317 SVGVMYEPEFAYHRQMLTKVFMLITTIDDIYDIYGTLEELQLFTTIVEKWDV-NRLEELP 376

Query: 526 EKVEIIFKALHSTVDEIGNKALTWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMD 585
             +++ F  L + +++IG   L  +G  V  ++ + W ++  +  +EA+W  +   P+ +
Sbjct: 377 NYMKLCFLCLVNEINQIGYFVLRDKGFNVIPYLKESWADMCTTFLKEAKWYKSGYKPNFE 436

Query: 586 DYMENGYISFALGPIVLPALYFIGPKLSEEVVGSFEYHNLFKLMSTCGRLLNDVQTIERE 645
           +YM+NG+IS ++ P +L  L+ +    + +++GS+  H++ +  +T  RL ND+ T   E
Sbjct: 437 EYMQNGWISSSV-PTILLHLFCLLSDQTLDILGSYN-HSVVRSSATILRLANDLATSSEE 489

Query: 646 SSEG-KLNALSLHMINTGGN 655
            + G  + ++  HM  TG +
Sbjct: 497 LARGDTMKSVQCHMHETGAS 489

BLAST of Sgr028675 vs. TAIR 10
Match: AT2G24210.1 (terpene synthase 10 )

HSP 1 Score: 154.5 bits (389), Expect = 3.4e-37
Identity = 114/435 (26.21%), Postives = 206/435 (47.36%), Query Frame = 0

Query: 226 QLCMVDSLQTLGIDHHFKEEIGRVLDKTYRYWLQGEENIL-LDASTCAMAFRMLRVYGYE 285
           QL  +D LQ LG+ +HF+ EI  +L  +Y+   +   NI   D    A+ FR+ R +G+ 
Sbjct: 98  QLEFIDDLQKLGVSYHFEAEIDNILTSSYK---KDRTNIQESDLHATALEFRLFRQHGFN 157

Query: 286 VSSDQLAQFSEDRFSDCLGGYLKDIGSVLELFKASQITIYPEDPVLENINSWTSHFLNYG 345
           VS D    F E    +C      DI  ++ L++AS ++   +  +   I  + +  L   
Sbjct: 158 VSEDVFDVFME----NCGKFDRDDIYGLISLYEASYLSTKLDKNLQIFIRPFATQQLRDF 217

Query: 346 LSSNSIHSDRLHRNMKQEVISALDFTYNTTLERLQYKSAIEHYSVNNIRILKTPYSCLNL 405
           + ++S + D    +M + V+ ALD  Y   + RL  +  I+ Y               N 
Sbjct: 218 VDTHS-NEDFGSCDMVEIVVQALDMPYYWQMRRLSTRWYIDVYGKRQ-----------NY 277

Query: 406 GHQDFLRLAMEDFNVCQCIHRQEKKDLERWVVENKLDKLI--ARQKLVYCYFTAAAAITS 465
            +   +  A  DFN+ Q IH++E K++  W +E  L K +  AR ++V  YF     I  
Sbjct: 278 KNLVVVEFAKIDFNIVQAIHQEELKNVSSWWMETGLGKQLYFARDRIVENYFWTIGQIQE 337

Query: 466 PELSDARLSWAKNGVLTTVVDDLFDVWGSEDERINLVQAVERWDVCNDMEYCSEKVEIIF 525
           P+    R +  K   L T +DD++D++G+ +E      A E WD+ N ++   E + + F
Sbjct: 338 PQYGYVRQTMTKINALLTTIDDIYDIYGTLEELQLFTVAFENWDI-NRLDELPEYMRLCF 397

Query: 526 KALHSTVDEIGNKALTWQGRCVTSHVADIWLELIRSMRQEAEWLSNKAVPSMDDYMENGY 585
             +++ V+ I  + L  +   V   +   W ++ ++   EA+W  +   P++++YM+N  
Sbjct: 398 LVIYNEVNSIACEILRTKNINVIPFLKKSWTDVSKAYLVEAKWYKSGHKPNLEEYMQNAR 457

Query: 586 ISFALGPIVLPALYFIGPKLSEEVVGSFEYH--NLFKLMSTCGRLLNDVQTIERESSEGK 645
           IS +   I +        +LS +V+ +   H  N+ +  S+  RL ND+ T   E + G 
Sbjct: 458 ISISSPTIFVHFYCVFSDQLSIQVLETLSQHQQNVVRCSSSVFRLANDLVTSPDELARGD 512

Query: 646 L-NALSLHMINTGGN 655
           +  ++  +M  TG +
Sbjct: 518 VCKSIQCYMSETGAS 512

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022147909.10.0e+0073.33ent-kaur-16-ene synthase, chloroplastic isoform X2 [Momordica charantia][more]
XP_022147908.10.0e+0073.13ent-kaur-16-ene synthase, chloroplastic isoform X1 [Momordica charantia][more]
XP_022147910.10.0e+0073.13ent-kaur-16-ene synthase, chloroplastic isoform X3 [Momordica charantia][more]
XP_016900364.10.0e+0072.11PREDICTED: ent-kaur-16-ene synthase, chloroplastic isoform X2 [Cucumis melo][more]
XP_008447073.10.0e+0071.78PREDICTED: ent-kaur-16-ene synthase, chloroplastic isoform X1 [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q395483.4e-29266.89Ent-kaur-16-ene synthase, chloroplastic OS=Cucurbita maxima OX=3661 PE=1 SV=1[more]
A0A1Z3GC641.8e-23754.90Ent-kaurene synthase 5, chloroplastic OS=Isodon rubescens OX=587669 GN=KSL5 PE=1... [more]
A0A2K9RFY03.1e-23755.19Ent-kaurene synthase TSP4, chloroplastic OS=Vitex agnus-castus OX=54477 GN=TPS4 ... [more]
A0A0U4CDK41.6e-23654.30Ent-kaur-16-ene synthase, chloroplastic OS=Salvia miltiorrhiza OX=226208 GN=KS P... [more]
A0A3G1QTS72.8e-23354.22Ent-kaurene synthase 1, chloroplastic OS=Isodon eriocalyx OX=662907 GN=KS1 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1D2F00.0e+0073.33ent-kaur-16-ene synthase, chloroplastic isoform X2 OS=Momordica charantia OX=367... [more]
A0A6J1D2L10.0e+0073.13ent-kaur-16-ene synthase, chloroplastic isoform X1 OS=Momordica charantia OX=367... [more]
A0A6J1D1G10.0e+0073.13ent-kaur-16-ene synthase, chloroplastic isoform X3 OS=Momordica charantia OX=367... [more]
A0A1S4DWJ80.0e+0072.11ent-kaur-16-ene synthase, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LO... [more]
A0A1S3BG060.0e+0071.78ent-kaur-16-ene synthase, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LO... [more]
Match NameE-valueIdentityDescription
AT1G79460.11.2e-22854.82Terpenoid cyclases/Protein prenyltransferases superfamily protein [more]
AT1G61120.11.2e-7729.13terpene synthase 04 [more]
AT4G02780.14.9e-7632.39Terpenoid cyclases/Protein prenyltransferases superfamily protein [more]
AT4G16740.13.3e-4027.27terpene synthase 03 [more]
AT2G24210.13.4e-3726.21terpene synthase 10 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001906Terpene synthase, N-terminal domainPFAMPF01397Terpene_synthcoord: 167..367
e-value: 2.3E-45
score: 154.7
IPR036965Terpene synthase, N-terminal domain superfamilyGENE3D1.50.10.130coord: 3..417
e-value: 6.8E-142
score: 475.3
IPR008949Isoprenoid synthase domain superfamilyGENE3D1.10.600.10Farnesyl Diphosphate Synthasecoord: 420..734
e-value: 1.6E-86
score: 292.2
IPR008949Isoprenoid synthase domain superfamilySUPERFAMILY48576Terpenoid synthasescoord: 407..730
IPR005630Terpene synthase, metal-binding domainPFAMPF03936Terpene_synth_Ccoord: 410..673
e-value: 9.4E-71
score: 238.2
NoneNo IPR availableGENE3D1.50.10.160coord: 8..208
e-value: 6.8E-142
score: 475.3
NoneNo IPR availableSFLDSFLDG01014Terpene_Cyclase_Like_1_N-termcoord: 5..400
e-value: 0.0
score: 326.6
NoneNo IPR availablePANTHERPTHR31739ENT-COPALYL DIPHOSPHATE SYNTHASE, CHLOROPLASTICcoord: 2..726
NoneNo IPR availablePANTHERPTHR31739:SF3ENT-KAUR-16-ENE SYNTHASE, CHLOROPLASTICcoord: 2..726
IPR044814Terpene cyclases, class 1, plantCDDcd00684Terpene_cyclase_plant_C1coord: 219..726
e-value: 4.14792E-164
score: 482.465
IPR008930Terpenoid cyclases/protein prenyltransferase alpha-alpha toroidSUPERFAMILY48239Terpenoid cyclases/Protein prenyltransferasescoord: 12..240
IPR008930Terpenoid cyclases/protein prenyltransferase alpha-alpha toroidSUPERFAMILY48239Terpenoid cyclases/Protein prenyltransferasescoord: 177..390

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr028675.1Sgr028675.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009686 gibberellin biosynthetic process
biological_process GO:0016102 diterpenoid biosynthetic process
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0009899 ent-kaurene synthase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0010333 terpene synthase activity