Sgr028602 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr028602 vs. NCBI nr
Match: KAG6598336.1 (Subtilisin-like protease 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1207.6 bits (3123), Expect = 0.0e+00
Identity = 622/779 (79.85%), Postives = 685/779 (87.93%), Query Frame = 0

Query: 1   MAAHNCLYFWFWFWFMAIFWVPSALTETDNYIVHMDMAAMPKPFASHHSWYSATLSSV-L 60
           MAAH  +  +FW   + IF   S LT+TDNYIVHMD+AAMPKPFA+HHSWYSATLSSV L
Sbjct: 1   MAAH--MRMFFWVSLIVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLL 60

Query: 61  DNSRLRITASSSSFSSSKLIHSYNHAINGFSASLTPSEHEALQKSPGYVSSVRDSSVEAD 120
           D S LR T SSSS S SKLIH Y HAINGF+A+LTPS+ +AL+ SPGYVSSVRDSSV AD
Sbjct: 61  DTSPLRTTTSSSSSSPSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRAD 120

Query: 121 TTHSSHFLGLNSNWGLLPISNYGSDVIIGLVDTGVWPESESFSDDGMSEIPSRWKGECES 180
           TTHSS+FL L+ N GLLPISNYGSDVIIG VDTGVWPESESF+DDG+ +IPSRWKGECES
Sbjct: 121 TTHSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGIPKIPSRWKGECES 180

Query: 181 GTQFAASLCNKKLIGARFFNKGLIAKFPNVTISMNSTRDTIGHGTHTSATAAGNYVNTAS 240
           GT F ASLCNKKLIG RFFNKGLIAKFPNVTISMNSTRDT GHGTHTS TAAG+YV  AS
Sbjct: 181 GTHFNASLCNKKLIGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEAS 240

Query: 241 FFGYGEGTARGVAPRARVAIYKAIWDEGNYLSDVIAAIDQAILDGVDVISLSLGLDGVPL 300
           FFGYG+GTARGVAPRARVAIYKAIW EGN +SDVIAAIDQAI DGVDVISLSLGLDGVPL
Sbjct: 241 FFGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPL 300

Query: 301 YEDPVAIATFAAVERGIFVATSAGNKGPQLGTVHNGAPWVLNVAAGTMDRDFGGTIILGD 360
           YEDPVAIATFAA+ERGIFVATSAGNKGPQ GTVH+GAPWVLNVAAGTMDRDFGGTI L +
Sbjct: 301 YEDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTN 360

Query: 361 GVSILGSSLFPVNSAMATSRLIPIVFMGGCYNLKKLKKVGYKIVVCEDKDD-SLSLQVDN 420
           GVS+LGSSLFP+N A+A S L PIVFMG C+NLKKLK+VG+KIVVCED D+ SL LQVDN
Sbjct: 361 GVSVLGSSLFPLNLAIALSPL-PIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDN 420

Query: 421 VQSAKVAGGIFISNISDWDNLIPTSFPTIFLTPDRGNIVKAYMKSSSNPKARVSFHKTIF 480
           V+SAK+A G+FISNISDWDNLI TSFP+IFL    GN++K Y+K SSNPKARV+FHKTI 
Sbjct: 421 VESAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTII 480

Query: 481 GRKPAPSVARYSSRGPSESCPFVLKPDITAPGDAILASWPQNVAATDVNSRPIYSKFNIR 540
           G KPAPSVARYSSRGPSESCPFVLKPDI APGDAILASWPQNVAATDV SRPIYSK+N+ 
Sbjct: 481 GTKPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKYNVL 540

Query: 541 SGTSMSCPHAAGVAALLKGAHPEWSPAAIRSAMMTTADVIDNTETQIKDLGNENRLATPL 600
           SGTSM+CPHAAGVAALLKGAHP WSPAAIRSAMMTTAD++DNT+T IKDLGN+N++ATPL
Sbjct: 541 SGTSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPL 600

Query: 601 AMGSGHVNPNKAVDPGLIYDMGLQDYVNLLCALNYTKIQIQTITRSASHTCSSPSLDLNY 660
           AMGSGHVNPNKA+DPGLIYDMG++DY NLLCALNYTK QIQTITRS S+ C  P LDLNY
Sbjct: 601 AMGSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKPLLDLNY 660

Query: 661 PSFIVIVNASDSKTS-TEISQEFKRRVTNLGEKATTYEAKLTPMNGLRVTVKPEKLEFKR 720
           PSFI+ VNASDS+T  TEIS+EFKRRVTN+GEK  TY AK+TPM GL VTV+P KL+FKR
Sbjct: 661 PSFIITVNASDSQTGRTEISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKR 720

Query: 721 KNHKLSFKLKIAGRRRRVKRETDVVFGYLSWVEVGGGHVARSPIVVAGGLKSKVRSHWN 777
           KN  LSFKLKI G    VKRE+DVVFGYL+WVEVGGGH  +SPIVVA  + + +RSHWN
Sbjct: 721 KNQNLSFKLKIRG-HVSVKRESDVVFGYLNWVEVGGGHRVQSPIVVA--VVAGLRSHWN 773

BLAST of Sgr028602 vs. NCBI nr
Match: KAG7029306.1 (Subtilisin-like protease SBT1.9, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1206.0 bits (3119), Expect = 0.0e+00
Identity = 622/779 (79.85%), Postives = 684/779 (87.80%), Query Frame = 0

Query: 1   MAAHNCLYFWFWFWFMAIFWVPSALTETDNYIVHMDMAAMPKPFASHHSWYSATLSSV-L 60
           MAAH  +  +FW   M IF   S LT+TDNYIVHMD+AAMP  FA+HHSWYSATLSSV L
Sbjct: 1   MAAH--MRMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPNSFATHHSWYSATLSSVLL 60

Query: 61  DNSRLRITASSSSFSSSKLIHSYNHAINGFSASLTPSEHEALQKSPGYVSSVRDSSVEAD 120
           D S LR T SSSS S SKLIH Y HAINGF+A+LTPS+ +AL+ SPGYVSSVRDSSV AD
Sbjct: 61  DTSPLRTTTSSSSSSPSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRAD 120

Query: 121 TTHSSHFLGLNSNWGLLPISNYGSDVIIGLVDTGVWPESESFSDDGMSEIPSRWKGECES 180
           TTHSS+FL L+ N GLLPISNYGSDVIIG VDTGVWPESESF+DDG+S+IPSRWKGECES
Sbjct: 121 TTHSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECES 180

Query: 181 GTQFAASLCNKKLIGARFFNKGLIAKFPNVTISMNSTRDTIGHGTHTSATAAGNYVNTAS 240
           GT F ASLCNKKLIG RFFNKGLIAKFPNVTISMNSTRDT GHGTHTS TAAG+YV  AS
Sbjct: 181 GTHFNASLCNKKLIGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEAS 240

Query: 241 FFGYGEGTARGVAPRARVAIYKAIWDEGNYLSDVIAAIDQAILDGVDVISLSLGLDGVPL 300
           FFGYG+GTARGVAPRARVAIYKAIW EGN +SDVIAAIDQAI DGVDVISLSLGLDGVPL
Sbjct: 241 FFGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPL 300

Query: 301 YEDPVAIATFAAVERGIFVATSAGNKGPQLGTVHNGAPWVLNVAAGTMDRDFGGTIILGD 360
           YEDPVAIATFAA+ERGIFVATSAGNKGPQ GTVH+GAPWVLNVAAGTMDRDFGGTI L +
Sbjct: 301 YEDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTN 360

Query: 361 GVSILGSSLFPVNSAMATSRLIPIVFMGGCYNLKKLKKVGYKIVVCEDKDD-SLSLQVDN 420
           GVS+LGSSLFP+N A+A S L PIVFMG C+NLKKLK+VG+KIVVCED D+ SL LQVDN
Sbjct: 361 GVSVLGSSLFPLNLAIALSPL-PIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDN 420

Query: 421 VQSAKVAGGIFISNISDWDNLIPTSFPTIFLTPDRGNIVKAYMKSSSNPKARVSFHKTIF 480
           V+SAK+A G+FISNISDWDNLI TSFP+IFL    GN++K Y+K SSNPKARV+FHKTI 
Sbjct: 421 VESAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTII 480

Query: 481 GRKPAPSVARYSSRGPSESCPFVLKPDITAPGDAILASWPQNVAATDVNSRPIYSKFNIR 540
           G KPAPSVARYSSRGPSESCPFVLKPDI APGDAILASWPQNVAATDV SRPIYSK+N+ 
Sbjct: 481 GTKPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKYNVL 540

Query: 541 SGTSMSCPHAAGVAALLKGAHPEWSPAAIRSAMMTTADVIDNTETQIKDLGNENRLATPL 600
           SGTSM+CPHAAGVAALLKGAHP WSPAAIRSAMMTTAD++DNT+T IKDLGN+N++ATPL
Sbjct: 541 SGTSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPL 600

Query: 601 AMGSGHVNPNKAVDPGLIYDMGLQDYVNLLCALNYTKIQIQTITRSASHTCSSPSLDLNY 660
           AMGSGHVNPNKA+DPGLIYDMG++DY NLLCALNYTK QIQTITRS S+ C  P LDLNY
Sbjct: 601 AMGSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKPLLDLNY 660

Query: 661 PSFIVIVNASDSKTS-TEISQEFKRRVTNLGEKATTYEAKLTPMNGLRVTVKPEKLEFKR 720
           PSFI+ VNASDS+T  TEIS+EFKRRVTN+GEK  TY AK+TPM GL VTV+P KL+FKR
Sbjct: 661 PSFIITVNASDSQTGRTEISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKR 720

Query: 721 KNHKLSFKLKIAGRRRRVKRETDVVFGYLSWVEVGGGHVARSPIVVAGGLKSKVRSHWN 777
           KN  LSFKLKI G    VKRE+DVVFGYL+WVEVGGGH  +SPIVVA  + + +RSHWN
Sbjct: 721 KNQNLSFKLKIRG-HVSVKRESDVVFGYLNWVEVGGGHRVQSPIVVA--VVAGLRSHWN 773

BLAST of Sgr028602 vs. NCBI nr
Match: XP_022131269.1 (subtilisin-like protease SBT1.9 [Momordica charantia])

HSP 1 Score: 1205.7 bits (3118), Expect = 0.0e+00
Identity = 624/779 (80.10%), Postives = 684/779 (87.80%), Query Frame = 0

Query: 4   HNCLYFWFWFWFMAIFW--VPSALTETDNYIVHMDMAAMPKPFASHHSWYSATLSSVLDN 63
           HNC  F+FWF F+AIFW  +PSA TETDNYIVHMD+AAMPKPFA+HH+WYSATLSSVLDN
Sbjct: 8   HNC--FYFWFLFIAIFWFLLPSASTETDNYIVHMDLAAMPKPFATHHTWYSATLSSVLDN 67

Query: 64  SRLRITASSSSFSSSKLIHSYNHAINGFSASLTPSEHEALQKSPGYVSSVRDSSVEADTT 123
            RL           SKLIH+YNHAINGFSAS+TPSE +ALQKSPGYVSSV DSS++ DTT
Sbjct: 68  PRL-------GMLPSKLIHTYNHAINGFSASITPSELKALQKSPGYVSSVPDSSLQVDTT 127

Query: 124 HSSHFLGLNSNWGLLPISNYGSDVIIGLVDTGVWPESESFSDDGMSEIPSRWKGECESGT 183
           HSSHFLGL+S+ G+L +SNYGSDVIIG VDTGVW ESESFSD+GM EIPSRWKG+CESGT
Sbjct: 128 HSSHFLGLSSDSGVL-LSNYGSDVIIGFVDTGVWAESESFSDEGMPEIPSRWKGKCESGT 187

Query: 184 QFAASLCNKKLIGARFFNKGLIAKFPNVTISMNSTRDTIGHGTHTSATAAGNYVNTASFF 243
                 CNKKLIGARFFN+GLIAKFPNVTISMN T DT GHGTHT+ATAAG YV  ASFF
Sbjct: 188 H-----CNKKLIGARFFNRGLIAKFPNVTISMNYTNDTNGHGTHTAATAAGGYVKDASFF 247

Query: 244 GYGEGTARGVAPRARVAIYKAIWDEGNYLSDVIAAIDQAILDGVDVISLSLGLDGVPLYE 303
           GYG GTARGVAPRARVAIYKAIW+EGNY+SDVIAAIDQAILD VDV+SLSLGLDG  LYE
Sbjct: 248 GYGRGTARGVAPRARVAIYKAIWEEGNYVSDVIAAIDQAILDSVDVLSLSLGLDGALLYE 307

Query: 304 DPVAIATFAAVERGIFVATSAGNKGPQLGTVHNGAPWVLNVAAGTMDRDFGGTIILGDGV 363
           DPVAIATFAAVERGI V TSAGNKGPQLGTVHNGAPWVLNVAAGTMDRDFGGTI LG+GV
Sbjct: 308 DPVAIATFAAVERGILVTTSAGNKGPQLGTVHNGAPWVLNVAAGTMDRDFGGTIRLGNGV 367

Query: 364 SILGSSLFPVNSAMATSRLIPIVFMGGCYNLKKLKKVGYKIVVCEDKDD----SLSLQVD 423
           S+LGSSLFP+NS MATS+L P+VFMGGCYNLKKLKKVG+KIVVCEDKD+    SLSLQVD
Sbjct: 368 SVLGSSLFPLNSGMATSKL-PLVFMGGCYNLKKLKKVGHKIVVCEDKDEYNSYSLSLQVD 427

Query: 424 NVQSAKVAGGIFISNISDWDNLIPTSFPTIFLTPDRGNIVKAYMKSSSNPKARVSFHKTI 483
           NV++AKV  G+FISN+SDWDN + TSFP+IFL+P  GNI+K Y+++SSNPKARVSFHKTI
Sbjct: 428 NVETAKVGVGVFISNLSDWDNSVQTSFPSIFLSPSNGNIIKHYIRTSSNPKARVSFHKTI 487

Query: 484 FGRKPAPSVARYSSRGPSESCPFVLKPDITAPGDAILASWPQNVAATDVNSRPIYSKFNI 543
           FGRKPAPSVARYSSRGP+ESCPFVLKPDI APGDAILASWP  VAATDVNSRPIYSKFN+
Sbjct: 488 FGRKPAPSVARYSSRGPAESCPFVLKPDIMAPGDAILASWPLKVAATDVNSRPIYSKFNV 547

Query: 544 RSGTSMSCPHAAGVAALLKGAHPEWSPAAIRSAMMTTADVIDNTETQIKDLGNENRLATP 603
            SGTSMSCPHAAGVAALLK AHP WSPAAIRSAMMTTADV+DNT+T IKDLG +N+LATP
Sbjct: 548 LSGTSMSCPHAAGVAALLKAAHPRWSPAAIRSAMMTTADVVDNTQTPIKDLGEKNKLATP 607

Query: 604 LAMGSGHVNPNKAVDPGLIYDMGLQDYVNLLCALNYTKIQIQTITRSASHTCSSPSLDLN 663
           LAMGSGHVNPNKAVDPGLIYDM +QDYVN+LCALNY K QIQTITRSAS+ C +PSLDLN
Sbjct: 608 LAMGSGHVNPNKAVDPGLIYDMEIQDYVNVLCALNYNKNQIQTITRSASNNCKNPSLDLN 667

Query: 664 YPSFIVIVNASDSKTSTEISQEFKRRVTNLGEKATTYEAKLTPM--NGLRVTVKPEKLEF 723
           YPSFIVIVNASDS T  EIS+EFKR VTN+ E+ATTYEAKLTPM  NG+RVTVKP KLEF
Sbjct: 668 YPSFIVIVNASDSNTEMEISREFKRTVTNVVEEATTYEAKLTPMNGNGVRVTVKPHKLEF 727

Query: 724 KRKNHKLSFKLKIAGRRRRVKRETDVVFGYLSWVEVGGGHVARSPIVVAGGLKSKVRSH 775
           K KNHKLSF +K+      V+R+ +VVF YLSWVEVGGGHVARSPIVV   L   +RSH
Sbjct: 728 KGKNHKLSFTVKVQLGHWIVQRDPNVVFCYLSWVEVGGGHVARSPIVVT-DLNFMLRSH 769

BLAST of Sgr028602 vs. NCBI nr
Match: XP_022962414.1 (subtilisin-like protease SBT1.9 [Cucurbita moschata])

HSP 1 Score: 1203.3 bits (3112), Expect = 0.0e+00
Identity = 622/780 (79.74%), Postives = 686/780 (87.95%), Query Frame = 0

Query: 1   MAAHNCLYFWFWFWFMAIFWVPSALTETDNYIVHMDMAAMPKPFASHHSWYSATLSSV-L 60
           MAAH  +  +FW   M IF   S LT+TDNYIVHMD+AAMPKPFA+HHSWYSATLSSV L
Sbjct: 1   MAAH--MRMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLL 60

Query: 61  DNSRLRITASSSSFS-SSKLIHSYNHAINGFSASLTPSEHEALQKSPGYVSSVRDSSVEA 120
           D S LR T SSSS S  SKLIH Y HAINGF+ASLTPS+ +AL+ SPGYVSS+RDSSV A
Sbjct: 61  DTSPLRTTTSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRA 120

Query: 121 DTTHSSHFLGLNSNWGLLPISNYGSDVIIGLVDTGVWPESESFSDDGMSEIPSRWKGECE 180
           DTTHSS+FL L+ N GLLPISNYGSDVIIG VDTGVWPESESF+DDG+S+IPSRWKGECE
Sbjct: 121 DTTHSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECE 180

Query: 181 SGTQFAASLCNKKLIGARFFNKGLIAKFPNVTISMNSTRDTIGHGTHTSATAAGNYVNTA 240
           SGT F ASLCNKKLIG RFFNKGLI+KFPNVTISMNSTRDT GHGTHTS TAAG+YV  A
Sbjct: 181 SGTHFNASLCNKKLIGGRFFNKGLISKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEA 240

Query: 241 SFFGYGEGTARGVAPRARVAIYKAIWDEGNYLSDVIAAIDQAILDGVDVISLSLGLDGVP 300
           SFFGYG+GTARGVAPRARVAIYKAIW EGN +SDVIAAIDQAI DGVDVISLSLGLDGVP
Sbjct: 241 SFFGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVP 300

Query: 301 LYEDPVAIATFAAVERGIFVATSAGNKGPQLGTVHNGAPWVLNVAAGTMDRDFGGTIILG 360
           LYEDPVAIATFAA+ERGIFVATSAGNKGPQ GTVH+GAPWVLNVAAGTMDRDFGGTI L 
Sbjct: 301 LYEDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLT 360

Query: 361 DGVSILGSSLFPVNSAMATSRLIPIVFMGGCYNLKKLKKVGYKIVVCEDKDD-SLSLQVD 420
           +GVS+LGSSLFP+NSA+A S L PIVFMG C+NLKKLK+VG+KIVVCED D+ SL LQVD
Sbjct: 361 NGVSVLGSSLFPLNSAIALSPL-PIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVD 420

Query: 421 NVQSAKVAGGIFISNISDWDNLIPTSFPTIFLTPDRGNIVKAYMKSSSNPKARVSFHKTI 480
           NV+SAK+A G+FISNISDWDNLI TSFP+IFL    GN++K Y+K SSNPKARV+FHKTI
Sbjct: 421 NVESAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTI 480

Query: 481 FGRKPAPSVARYSSRGPSESCPFVLKPDITAPGDAILASWPQNVAATDVNSRPIYSKFNI 540
            G KPAPSVARYSSRGPSESCPFVLKPDI APGDAILASWPQNVAATDV SRPIYSKFN+
Sbjct: 481 IGTKPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNV 540

Query: 541 RSGTSMSCPHAAGVAALLKGAHPEWSPAAIRSAMMTTADVIDNTETQIKDLGNENRLATP 600
            SGTSM+CPHAAGVAALLKGAHP WSPAAIRSAMMTTAD++DNT+T IKDLGN+N++ATP
Sbjct: 541 LSGTSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATP 600

Query: 601 LAMGSGHVNPNKAVDPGLIYDMGLQDYVNLLCALNYTKIQIQTITRSASHTCSSPSLDLN 660
           LAMGSGHVNPNKA+DPGLIYDMG++DY NLLCALNYTK QIQTITRS S+ C    LDLN
Sbjct: 601 LAMGSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLN 660

Query: 661 YPSFIVIVNASDSKTS-TEISQEFKRRVTNLGEKATTYEAKLTPMNGLRVTVKPEKLEFK 720
           YPSFI+ VNASDS+T  TE+S+EFKRRVTN+GEK  TY AK+TPM GL VTV+P KL+FK
Sbjct: 661 YPSFIMTVNASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFK 720

Query: 721 RKNHKLSFKLKIAGRRRRVKRETDVVFGYLSWVEVGGGHVARSPIVVAGGLKSKVRSHWN 777
           RKN  LSFKLKI G    +KRE+DVVFGYL+WVEVGGGH  +SPIVVA  + + +RSHWN
Sbjct: 721 RKNQNLSFKLKIRG-HVSIKRESDVVFGYLNWVEVGGGHRVQSPIVVA--VVAGLRSHWN 774

BLAST of Sgr028602 vs. NCBI nr
Match: XP_023546329.1 (subtilisin-like protease SBT1.9 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1197.2 bits (3096), Expect = 0.0e+00
Identity = 616/773 (79.69%), Postives = 678/773 (87.71%), Query Frame = 0

Query: 1   MAAHNCLYFWFWFWFMAIFWVPSALTETDNYIVHMDMAAMPKPFASHHSWYSATLSSV-L 60
           MAAH  +  +FW   M IF   S +TETDNYIVHMD+AAMPKPFA+HHSWYSATLSSV L
Sbjct: 1   MAAH--MRMFFWISLMVIFLARSTVTETDNYIVHMDLAAMPKPFATHHSWYSATLSSVLL 60

Query: 61  DNSRLRITASSSSFSSSKLIHSYNHAINGFSASLTPSEHEALQKSPGYVSSVRDSSVEAD 120
           D S LR T SSSS   SKLIH Y HAINGF+A+LTPS+ +AL+ SPGYVSSVRDSSV AD
Sbjct: 61  DTSPLRTTTSSSSSLPSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRAD 120

Query: 121 TTHSSHFLGLNSNWGLLPISNYGSDVIIGLVDTGVWPESESFSDDGMSEIPSRWKGECES 180
           TTHSS+FL L+ N GLLPISNYGSDVIIG VDTGVWPESESF+DDG+S+IPSRWKGECES
Sbjct: 121 TTHSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECES 180

Query: 181 GTQFAASLCNKKLIGARFFNKGLIAKFPNVTISMNSTRDTIGHGTHTSATAAGNYVNTAS 240
           GT F ASLCNKKLIG RFFNKGLIAKFPNVTISMNSTRDT GHGTHTS TAAG+YV  AS
Sbjct: 181 GTHFNASLCNKKLIGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEAS 240

Query: 241 FFGYGEGTARGVAPRARVAIYKAIWDEGNYLSDVIAAIDQAILDGVDVISLSLGLDGVPL 300
           FFGYG+GTARGVAPRARVAIYKAIW EGN +SDVIAAIDQAI DGVDVI+LSLG+D VPL
Sbjct: 241 FFGYGQGTARGVAPRARVAIYKAIWKEGNVVSDVIAAIDQAISDGVDVIALSLGIDRVPL 300

Query: 301 YEDPVAIATFAAVERGIFVATSAGNKGPQLGTVHNGAPWVLNVAAGTMDRDFGGTIILGD 360
           YEDPVAIATFAA+ERGIFVATSAGNKGPQ GTVH+GAPWVLNVAAGTMDRDFGGTI L +
Sbjct: 301 YEDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTN 360

Query: 361 GVSILGSSLFPVNSAMATSRLIPIVFMGGCYNLKKLKKVGYKIVVCEDKDD-SLSLQVDN 420
           GVS+LGSSLFP+NSA+A S L PI FMG C+NLKKLK+VG+KIVVCED D+ SL LQVDN
Sbjct: 361 GVSVLGSSLFPLNSAIALSPL-PIFFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDN 420

Query: 421 VQSAKVAGGIFISNISDWDNLIPTSFPTIFLTPDRGNIVKAYMKSSSNPKARVSFHKTIF 480
           VQSAK+A G+FISNISDWDNLI TSFP+IFL    GN++K Y+  SSNPKARV+FHKTI 
Sbjct: 421 VQSAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVLKDYINRSSNPKARVNFHKTII 480

Query: 481 GRKPAPSVARYSSRGPSESCPFVLKPDITAPGDAILASWPQNVAATDVNSRPIYSKFNIR 540
           G KPAPSVARYSSRGPSESCPFVLKPDI APGDAILASWPQNVAATDV SRPIYSKFN+ 
Sbjct: 481 GTKPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVL 540

Query: 541 SGTSMSCPHAAGVAALLKGAHPEWSPAAIRSAMMTTADVIDNTETQIKDLGNENRLATPL 600
           SGTSM+CPHAAGVAALLKGAHP WSPAAIRSAMMTTAD++DNT+T IKDLGN+N++ATPL
Sbjct: 541 SGTSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPL 600

Query: 601 AMGSGHVNPNKAVDPGLIYDMGLQDYVNLLCALNYTKIQIQTITRSASHTCSSPSLDLNY 660
           AMGSGHVNPNKA+DPGLIYDMG++DY NL+CALNYTK QIQTITRS S+ C  PSLDLNY
Sbjct: 601 AMGSGHVNPNKAIDPGLIYDMGIEDYTNLICALNYTKNQIQTITRSTSNDCEKPSLDLNY 660

Query: 661 PSFIVIVNASDSKTS-TEISQEFKRRVTNLGEKATTYEAKLTPMNGLRVTVKPEKLEFKR 720
           PSFI+ VNASDS+T  TEIS+EFKRRVTN+GEK  TY AK+TPM GL VTV+P+KL+FKR
Sbjct: 661 PSFIITVNASDSETGRTEISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPKKLKFKR 720

Query: 721 KNHKLSFKLKIAGRRRRVKRETDVVFGYLSWVEVGGGHVARSPIVVAGGLKSK 771
           KN  LSFKLKI G    VKRE+DVVFGYL+WVEVGGGH  +SPIVV     S+
Sbjct: 721 KNQNLSFKLKIRG-HVSVKRESDVVFGYLNWVEVGGGHRVQSPIVVTNMSSSR 769

BLAST of Sgr028602 vs. ExPASy Swiss-Prot
Match: O82777 (Subtilisin-like protease SBT3 OS=Solanum lycopersicum OX=4081 GN=sbt3 PE=1 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 4.5e-194
Identity = 369/752 (49.07%), Postives = 500/752 (66.49%), Query Frame = 0

Query: 24  ALTETDNYIVHMDMAAMPKPFASHHSWYSATLSSVLDNSRLRITASSSSF-SSSKLIHSY 83
           AL +   YIVH+D + MP  F  HH W+S+T+ S+    +  + +S   F S+ KL++SY
Sbjct: 20  ALAQRSTYIVHLDKSLMPNVFTDHHHWHSSTIDSI----KASVPSSVDRFHSAPKLVYSY 79

Query: 84  NHAINGFSASLTPSEHEALQKSPGYVSSVRDSSVEADTTHSSHFLGLNSNWGLLPISNYG 143
           ++ ++GFSA L+  E  AL+K PG++S+ +D +VE  TTH+S FL LN + GL P S  G
Sbjct: 80  DNVLHGFSAVLSKDELAALKKLPGFISAYKDRTVEPHTTHTSDFLKLNPSSGLWPASGLG 139

Query: 144 SDVIIGLVDTGVWPESESFSDDGMSEIPSRWKGECESGTQFAASLCNKKLIGARFFNKGL 203
            DVI+ ++D+G+WPES SF DDGM EIP RWKG C+ GTQF AS+CN+KLIGA +FNKG+
Sbjct: 140 QDVIVAVLDSGIWPESASFQDDGMPEIPKRWKGICKPGTQFNASMCNRKLIGANYFNKGI 199

Query: 204 IAKFPNVTISMNSTRDTIGHGTHTSATAAGNYVNTASFFGYGEGTARGVAPRARVAIYKA 263
           +A  P V I+MNS RDT GHGTH ++  AGN+    S FGY  GTARGVAPRAR+A+YK 
Sbjct: 200 LANDPTVNITMNSARDTDGHGTHCASITAGNFAKGVSHFGYAPGTARGVAPRARLAVYKF 259

Query: 264 IWDEGNYLSDVIAAIDQAILDGVDVISLSLGLDGVPLYEDPVAIATFAAVERGIFVATSA 323
            ++EG + SD+IAA+DQA+ DGVD+IS+S G   +PLYED ++IA+F A+ +G+ V+ SA
Sbjct: 260 SFNEGTFTSDLIAAMDQAVADGVDMISISYGYRFIPLYEDAISIASFGAMMKGVLVSASA 319

Query: 324 GNKGPQLGTVHNGAPWVLNVAAGTMDRDFGGTIILGDGVSILGSSLFPVNSAMATSRLIP 383
           GN+GP +G+++NG+PW+L VA+G  DR F GT+ LG+G+ I G SLFP  + +  S +I 
Sbjct: 320 GNRGPGIGSLNNGSPWILCVASGHTDRTFAGTLTLGNGLKIRGWSLFPARAFVRDSPVIY 379

Query: 384 IVFMGGCYN---LKKLKKVGYKIVVCEDKDDSLSLQVDNVQSAKVAGGIFISNISDWDNL 443
              +  C +   L +++     IV+C+D  D  S Q+  +  A++   IFIS        
Sbjct: 380 NKTLSDCSSEELLSQVENPENTIVICDDNGD-FSDQMRIITRARLKAAIFISEDPGVFRS 439

Query: 444 IPTSFPTIFLTPDRGNIVKAYMKSSSNPKARVSFHKTIFGRKPAPSVARYSSRGPSESCP 503
                P + +    G  V  Y+K+S  P A ++F +T    KPAP VA  S+RGPS S  
Sbjct: 440 ATFPNPGVVVNKKEGKQVINYVKNSVTPTATITFQETYLDTKPAPVVAASSARGPSRSYL 499

Query: 504 FVLKPDITAPGDAILASWPQNVAATDVNSRPIYS-KFNIRSGTSMSCPHAAGVAALLKGA 563
            + KPDI APG  ILA++P NV AT + +  + S  + + SGTSM+ PHAAG+AA+LK A
Sbjct: 500 GISKPDILAPGVLILAAYPPNVFATSIGTNILLSTDYILESGTSMAAPHAAGIAAMLKAA 559

Query: 564 HPEWSPAAIRSAMMTTADVIDNTETQIKDLGNENRLATPLAMGSGHVNPNKAVDPGLIYD 623
           HPEWSP+AIRSAMMTTAD +DNT   IKD  N N+ ATPL MG+GHV+PN+A+DPGL+YD
Sbjct: 560 HPEWSPSAIRSAMMTTADPLDNTRKPIKDSDN-NKAATPLDMGAGHVDPNRALDPGLVYD 619

Query: 624 MGLQDYVNLLCALNYTKIQIQTITR-SASHTCSSPSLDLNYPSFIVIVNASDSKTSTEIS 683
              QDYVNLLC+LN+T+ Q +TI R SASH CS+PS DLNYPSFI + +   + T  E  
Sbjct: 620 ATPQDYVNLLCSLNFTEEQFKTIARSSASHNCSNPSADLNYPSFIALYSIEGNFTLLE-- 679

Query: 684 QEFKRRVTNLGEKATTYEAKLTPMNGLRVTVKPEKLEFKRKNHKLSFKLKI-----AGRR 743
           Q+FKR VTN+G+ A TY+AKL       ++V P+ L FK KN K S+ L I      G+ 
Sbjct: 680 QKFKRTVTNVGKGAATYKAKLKAPKNSTISVSPQILVFKNKNEKQSYTLTIRYIGDEGQS 739

Query: 744 RRVKRETDVVFGYLSWVEVGGGHVARSPIVVA 765
           R V        G ++WVE  G H  RSPIV +
Sbjct: 740 RNV--------GSITWVEQNGNHSVRSPIVTS 755

BLAST of Sgr028602 vs. ExPASy Swiss-Prot
Match: Q9FHA4 (Subtilisin-like protease SBT1.9 OS=Arabidopsis thaliana OX=3702 GN=SBT1.9 PE=2 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 4.3e-168
Identity = 339/767 (44.20%), Postives = 468/767 (61.02%), Query Frame = 0

Query: 19  FWVPSALTETDNYIVHMDMAAMPKPFASHHSWYSATLSSVLDNSRLRITASSSSFSSSKL 78
           F+V     ET  YI+HMD++A P PF+ H SW+S TL+SV+ N +             K+
Sbjct: 13  FFVAIVTAETSPYIIHMDLSAKPLPFSDHRSWFSTTLTSVITNRK------------PKI 72

Query: 79  IHSYNHAINGFSASLTPSEHEALQKSPGYVSSVRDSSVEADTTHSSHFLGLNSNWGLLPI 138
           I++Y  +++GFSA LT SE + L+  PGYVS  +D  V+  TT S  F+GLNS  G  P+
Sbjct: 73  IYAYTDSVHGFSAVLTNSELQRLKHKPGYVSFTKDLPVKLHTTFSPKFIGLNSTSGTWPV 132

Query: 139 SNYGSDVIIGLVDTGVWPESESFSDDGMSEIPSRWKGECESGTQFAASLCNKKLIGARFF 198
           SNYG+ ++IG++DTG+WP+S SF DDG+  +PS+WKG CE     ++SLCNKKLIGA+ F
Sbjct: 133 SNYGAGIVIGIIDTGIWPDSPSFHDDGVGSVPSKWKGACEFN---SSSLCNKKLIGAKVF 192

Query: 199 NKGLIAKFPNVTIS----MNSTRDTIGHGTHTSATAAGNYVNTASFFGYGEGTARGVAPR 258
           NKGL A  P++  +     +S  DTIGHGTH +A AAGN+V  AS+F Y +GTA G+AP 
Sbjct: 193 NKGLFANNPDLRETKIGQYSSPYDTIGHGTHVAAIAAGNHVKNASYFSYAQGTASGIAPH 252

Query: 259 ARVAIYKAIWDEGNYLSDVIAAIDQAILDGVDVISLSLGL--------DGVPLYEDPVAI 318
           A +AIYKA W+EG Y SDVIAAIDQAI DGV VISLSLGL        DG  L  DP+A+
Sbjct: 253 AHLAIYKAAWEEGIYSSDVIAAIDQAIRDGVHVISLSLGLSFEDDDDNDGFGLENDPIAV 312

Query: 319 ATFAAVERGIFVATSAGNKGPQLGTVHNGAPWVLNVAAGTMDRDFGGTIILGDGVSILGS 378
           A+FAA+++G+FV TS GN GP   ++ NGAPW++ V AGT+ R F GT+  G+ VS    
Sbjct: 313 ASFAAIQKGVFVVTSGGNDGPYYWSLINGAPWIMTVGAGTIGRQFQGTLTFGNRVSFSFP 372

Query: 379 SLFPVNSAMATSRLIPIVFM--GGCYNLKKLKKVGYKIVVCEDKDDSLSLQVDNVQSAKV 438
           SLFP       S   P+ ++  G   N    K +  +IVVC +++ ++  ++  ++S   
Sbjct: 373 SLFP---GEFPSVQFPVTYIESGSVEN----KTLANRIVVC-NENINIGSKLHQIRSTGA 432

Query: 439 AGGIFISN-ISDWDNLIPTSFPTIFLTPDRGNIVKAYMKSS-SNPKARVSFHKTIFGRKP 498
           A  + I++ + +  + I   FP  F+       +++Y  S+ +N  A++ F KT+ G KP
Sbjct: 433 AAVVLITDKLLEEQDTIKFQFPVAFIGSKHRETIESYASSNKNNATAKLEFRKTVIGTKP 492

Query: 499 APSVARYSSRGPSESCPFVLKPDITAPGDAILASWPQNVAATDVNSRPIYSKFNIRSGTS 558
           AP V  YSSRGP  S P +LKPDI APG  IL++WP     T   + P++S FN+ +GTS
Sbjct: 493 APEVGTYSSRGPFTSFPQILKPDILAPGTLILSAWPSVEQITGTRALPLFSGFNLLTGTS 552

Query: 559 MSCPHAAGVAALLKGAHPEWSPAAIRSAMMTTADVIDNTETQIKDLGNENRLATPLAMGS 618
           M+ PH AGVAAL+K  HP WSP+AI+SA+MTTA  +DN                PLA+G+
Sbjct: 553 MAAPHVAGVAALIKQVHPNWSPSAIKSAIMTTALTLDN----------------PLAVGA 612

Query: 619 GHVNPNKAVDPGLIYDMGLQDYVNLLC-ALNYTKIQIQTITRS-ASHTCSSPSLDLNYPS 678
           GHV+ NK ++PGLIYD   QD++N LC     ++  I  ITRS  S  C  PS  LNYPS
Sbjct: 613 GHVSTNKVLNPGLIYDTTPQDFINFLCHEAKQSRKLINIITRSNISDACKKPSPYLNYPS 672

Query: 679 FIVIVNASDSKTSTEISQEFKRRVTNLGEKATTYEAKLTPMNGLRVTVKPEKLEFKRKNH 738
            I    +  S         FKR +TN+GE   +Y  ++  + GL V V+P+KL F  KN 
Sbjct: 673 IIAYFTSDQSSPKI-----FKRTLTNVGEAKRSYIVRVRGLKGLNVVVEPKKLMFSEKNE 732

Query: 739 KLSFKLKIAGRRRRVKRETDVVFGYLSWVEVGGGHVARSPIVVAGGL 768
           KLS+ +++   R     + +VV+G +SWV+        S  VVA  L
Sbjct: 733 KLSYTVRLESPR---GLQENVVYGLVSWVDEDEAEFEVSCSVVATSL 732

BLAST of Sgr028602 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 1.1e-150
Identity = 320/764 (41.88%), Postives = 448/764 (58.64%), Query Frame = 0

Query: 21  VPSALTETDNYIVHMDMAAMPKPFASHHSWYSATLSSVLDNSRLRITASSSSFSSSKLIH 80
           V S+ ++   YIVHM  + MP  F  H +WY ++L S+ D              S++L++
Sbjct: 22  VSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISD--------------SAELLY 81

Query: 81  SYNHAINGFSASLTPSEHEALQKSPGYVSSVRDSSVEADTTHSSHFLGLNSNWG-LLPIS 140
           +Y +AI+GFS  LT  E ++L   PG +S + +   E  TT +  FLGL+ +   L P +
Sbjct: 82  TYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEA 141

Query: 141 NYGSDVIIGLVDTGVWPESESFSDDGMSEIPSRWKGECESGTQFAASLCNKKLIGARFFN 200
              SDV++G++DTGVWPES+S+SD+G   IPS WKG CE+GT F ASLCN+KLIGARFF 
Sbjct: 142 GSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFA 201

Query: 201 KGLIAKFPNVTIS--MNSTRDTIGHGTHTSATAAGNYVNTASFFGYGEGTARGVAPRARV 260
           +G  +    +  S    S RD  GHGTHTS+TAAG+ V  AS  GY  GTARG+APRARV
Sbjct: 202 RGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARV 261

Query: 261 AIYKAIWDEGNYLSDVIAAIDQAILDGVDVISLSLGLDGVPLYEDPVAIATFAAVERGIF 320
           A+YK  W  G + SD++AAID+AI D V+V+S+SLG      Y D VAI  FAA+ERGI 
Sbjct: 262 AVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGIL 321

Query: 321 VATSAGNKGPQLGTVHNGAPWVLNVAAGTMDRDFGGTIILGDGVSILGSSLFPVNSAMAT 380
           V+ SAGN GP   ++ N APW+  V AGT+DRDF    ILG+G +  G SLF        
Sbjct: 322 VSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLF--KGEALP 381

Query: 381 SRLIPIVFMGGCYNLKK----------LKKVGYKIVVCEDKDDSLSLQVDNVQSAKVAGG 440
            +L+P ++ G   N              +KV  KIV+C+   ++   + D V++A   G 
Sbjct: 382 DKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGM 441

Query: 441 IFISNISDWDNLIPTS--FPTIFLTPDRGNIVKAYMKSSSNPKARVSFHKTIFGRKPAPS 500
           I  +  ++ + L+  +   P   +    G+I++ Y+ +  NP A +S   T+ G KP+P 
Sbjct: 442 ILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPV 501

Query: 501 VARYSSRGPSESCPFVLKPDITAPGDAILASWPQNVAATDVNSRPIYSKFNIRSGTSMSC 560
           VA +SSRGP+   P +LKPD+ APG  ILA+W      T + S     +FNI SGTSMSC
Sbjct: 502 VAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSC 561

Query: 561 PHAAGVAALLKGAHPEWSPAAIRSAMMTTADVIDNTETQIKDLGNENRLATPLAMGSGHV 620
           PH +G+AALLK  HPEWSPAAIRSA+MTTA         + D+    + +TP   G+GHV
Sbjct: 562 PHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIAT-GKPSTPFDHGAGHV 621

Query: 621 NPNKAVDPGLIYDMGLQDYVNLLCALNYTKIQIQTITRSASHTC----SSPSLDLNYPSF 680
           +P  A +PGLIYD+  +DY+  LCALNYT  QI++++R  ++TC    S    DLNYPSF
Sbjct: 622 SPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSR-RNYTCDPSKSYSVADLNYPSF 681

Query: 681 IVIVNASDSKTSTEISQEFKRRVTNLGEKATTYEAKLT-PMNGLRVTVKPEKLEFKRKNH 740
            V V+          + ++ R VT++G  A TY  K+T    G++++V+P  L FK  N 
Sbjct: 682 AVNVDGVG-------AYKYTRTVTSVG-GAGTYSVKVTSETTGVKISVEPAVLNFKEANE 741

Query: 741 KLSFKLKIAGRRRRVKRETDVVFGYLSWVEVGGGHVARSPIVVA 765
           K S+ +       +        FG + W +  G HV  SP+ ++
Sbjct: 742 KKSYTVTFTVDSSKPSGSNS--FGSIEWSD--GKHVVGSPVAIS 755

BLAST of Sgr028602 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 7.6e-149
Identity = 326/788 (41.37%), Postives = 457/788 (57.99%), Query Frame = 0

Query: 9   FWFWFWFMAIFWVPSALTETDN---YIVHMDMAAMPKPFASHHSWYSATLSSVLDNSRLR 68
           F+F+F+F+     PS+   + N   YIVH+D  A P  F +H  WY+++L+S        
Sbjct: 3   FFFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLAS-------- 62

Query: 69  ITASSSSFSSSKLIHSYNHAINGFSASLTPSEHEALQKSPGYVSSVRDSSVEADTTHSSH 128
           +T+S  S     +IH+Y+   +GFSA LT  +   L   P  +S + +      TT S  
Sbjct: 63  LTSSPPS-----IIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPE 122

Query: 129 FLGLNS--NWGLLPISNYGSDVIIGLVDTGVWPESESFSDDGMSEIPSRWKGECESGTQF 188
           FLGL S    GLL  S++GSD++IG++DTGVWPE  SF D G+  +P +WKG+C +   F
Sbjct: 123 FLGLRSTDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDF 182

Query: 189 AASLCNKKLIGARFFNKGLIAK--FPNVTISMNSTRDTIGHGTHTSATAAGNYVNTASFF 248
             S CN+KL+GARFF  G  A     N T    S RD+ GHGTHT++ +AG YV  AS  
Sbjct: 183 PESACNRKLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTL 242

Query: 249 GYGEGTARGVAPRARVAIYKAIWDEGNYLSDVIAAIDQAILDGVDVISLSLGLDGVPLYE 308
           GY  G A G+AP+AR+A YK  W+ G Y SD++AA D A+ DGVDVISLS+G   VP Y 
Sbjct: 243 GYAHGVAAGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYL 302

Query: 309 DPVAIATFAAVERGIFVATSAGNKGPQLGTVHNGAPWVLNVAAGTMDRDFGGTIILGDGV 368
           D +AI  F A++RGIFV+ SAGN GP   TV N APW+  V AGT+DRDF   + LG+G 
Sbjct: 303 DAIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGK 362

Query: 369 SILGSSLFPVNSAMATSRLIPIVF----MGGCYNLKKL--------KKVGYKIVVCEDKD 428
            I G S++     +   R+ P+V+    +GG      L          V  KIV+C+   
Sbjct: 363 MISGVSVYG-GPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGI 422

Query: 429 DSLSLQVDNVQSAKVAGGIFISNISDWDNLIPTS--FPTIFLTPDRGNIVKAYM------ 488
           +S + + + V+     G I  + + D + L+      P   +    G+ ++ Y+      
Sbjct: 423 NSRATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKS 482

Query: 489 KSSSNPKARVSFHKTIFGRKPAPSVARYSSRGPSESCPFVLKPDITAPGDAILASWPQNV 548
           +SS +P A + F  T  G +PAP VA +S+RGP+   P +LKPD+ APG  ILA+WP  +
Sbjct: 483 RSSKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRI 542

Query: 549 AATDVNSRPIYSKFNIRSGTSMSCPHAAGVAALLKGAHPEWSPAAIRSAMMTTADVIDNT 608
             + V S    ++FNI SGTSM+CPH +G+AALLK AHP+WSPAAIRSA++TTA  +DN+
Sbjct: 543 GPSGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNS 602

Query: 609 ETQIKDLGNENRLATPLAMGSGHVNPNKAVDPGLIYDMGLQDYVNLLCALNYTKIQIQTI 668
              + D    N  ++ +  GSGHV+P KA+DPGL+YD+   DY+N LC  NYT+  I TI
Sbjct: 603 GEPMMDESTGN-TSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTI 662

Query: 669 TRSASHTCSSPSL----DLNYPSF-IVIVNASDSKTSTEISQEFKRRVTNLGEKATTYEA 728
           TR  +    +       +LNYPSF +V     +SK ST     F R VTN+G+  + YE 
Sbjct: 663 TRRQADCDGARRAGHVGNLNYPSFSVVFQQYGESKMST----HFIRTVTNVGDSDSVYEI 722

Query: 729 KLTPMNGLRVTVKPEKLEFKRKNHKLSFKLKIAGRRRRVK-RETDVVFGYLSWVEVGGGH 764
           K+ P  G  VTV+PEKL F+R   KLSF +++     ++    T+V  G++ W +  G  
Sbjct: 723 KIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSD--GKR 769

BLAST of Sgr028602 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 6.4e-148
Identity = 319/758 (42.08%), Postives = 435/758 (57.39%), Query Frame = 0

Query: 31  YIVHMDMAAMPKPFASHHSWYSATLSSVLDNSRLRITASSSSFSSSKLIHSYNHAINGFS 90
           +I  +D  +MP  F +H+ WYS   +                   S+++H Y+   +GFS
Sbjct: 28  FIFRIDGGSMPSIFPTHYHWYSTEFA-----------------EESRIVHVYHTVFHGFS 87

Query: 91  ASLTPSEHEALQKSPGYVSSVRDSSVEADTTHSSHFLGLNSNWGLLPISNYGSDVIIGLV 150
           A +TP E + L+  P  ++   D   E  TT S  FLGL +  GL   S+YGSDVIIG+ 
Sbjct: 88  AVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYGSDVIIGVF 147

Query: 151 DTGVWPESESFSDDGMSEIPSRWKGECESGTQFAASLCNKKLIGARFFNKGLIAKF---P 210
           DTG+WPE  SFSD  +  IP RW+G CESG +F+   CN+K+IGARFF KG  A      
Sbjct: 148 DTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQAAVIGGI 207

Query: 211 NVTISMNSTRDTIGHGTHTSATAAGNYVNTASFFGYGEGTARGVAPRARVAIYKAIW-DE 270
           N T+   S RD  GHGTHTS+TAAG +   AS  GY  G A+GVAP+AR+A YK  W D 
Sbjct: 208 NKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYKVCWKDS 267

Query: 271 GNYLSDVIAAIDQAILDGVDVISLSL-GLDGV--PLYEDPVAIATFAAVERGIFVATSAG 330
           G   SD++AA D A+ DGVDVIS+S+ G DG+  P Y DP+AI ++ A  +GIFV++SAG
Sbjct: 268 GCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGIFVSSSAG 327

Query: 331 NKGPQLGTVHNGAPWVLNVAAGTMDRDFGGTIILGDGVSILGSSLF---PVNSAMATSRL 390
           N+GP   +V N APWV  V A T+DR+F    ILGDG  + G SL+   P+N      R+
Sbjct: 328 NEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVPLN-----GRM 387

Query: 391 IPIVFMGG---------CYNLKKLKKVGYKIVVCEDKDDSLSLQVDNVQSAKVAGGIFIS 450
            P+V+ G            N    K+V  KIV+C+        +   V+ A   G I  +
Sbjct: 388 FPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILAN 447

Query: 451 NISDWDNLIPTS--FPTIFLTPDRGNIVKAYMKSSSNPKARVSFHKTIFGRKPAPSVARY 510
             S+ + L+  +   P   +  + G+ +KAY  S  NP A + F  TI G KPAP +A +
Sbjct: 448 GASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIASF 507

Query: 511 SSRGPSESCPFVLKPDITAPGDAILASWPQNVAATDVNSRPIYSKFNIRSGTSMSCPHAA 570
           S RGP+   P +LKPD+ APG  ILA+W   V  T + S P  ++FNI SGTSM+CPH +
Sbjct: 508 SGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVS 567

Query: 571 GVAALLKGAHPEWSPAAIRSAMMTTADVIDNTETQIKDLGNENRLATPLAMGSGHVNPNK 630
           G AALLK AHP+WSPA IRSAMMTT +++DN+   + D  +  + ATP   GSGH+N  +
Sbjct: 568 GAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLID-ESTGKSATPYDYGSGHLNLGR 627

Query: 631 AVDPGLIYDMGLQDYVNLLCALNYTKIQIQTITRSASH--TCSSPSL-DLNYPSFIVIVN 690
           A++PGL+YD+   DY+  LC++ Y    IQ ITR+     T   PS  +LNYPS   +  
Sbjct: 628 AMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVF- 687

Query: 691 ASDSKTSTEISQEFKRRVTNLGEKATTYEAKLTPMNGLRVTVKPEKLEFKRKNHKLSFKL 750
              +     +S+   R  TN+G+    Y A++    G+ VTVKP +L F     + S+ +
Sbjct: 688 --PTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAV 747

Query: 751 KI-AGRRRRVKRETDVVFGYLSWVEVGGGHVARSPIVV 764
            +    R  V  ET  VFG ++W + GG HV RSPIVV
Sbjct: 748 TVTVNTRNVVLGETGAVFGSVTWFD-GGKHVVRSPIVV 758

BLAST of Sgr028602 vs. ExPASy TrEMBL
Match: A0A6J1BPR9 (subtilisin-like protease SBT1.9 OS=Momordica charantia OX=3673 GN=LOC111004540 PE=3 SV=1)

HSP 1 Score: 1205.7 bits (3118), Expect = 0.0e+00
Identity = 624/779 (80.10%), Postives = 684/779 (87.80%), Query Frame = 0

Query: 4   HNCLYFWFWFWFMAIFW--VPSALTETDNYIVHMDMAAMPKPFASHHSWYSATLSSVLDN 63
           HNC  F+FWF F+AIFW  +PSA TETDNYIVHMD+AAMPKPFA+HH+WYSATLSSVLDN
Sbjct: 8   HNC--FYFWFLFIAIFWFLLPSASTETDNYIVHMDLAAMPKPFATHHTWYSATLSSVLDN 67

Query: 64  SRLRITASSSSFSSSKLIHSYNHAINGFSASLTPSEHEALQKSPGYVSSVRDSSVEADTT 123
            RL           SKLIH+YNHAINGFSAS+TPSE +ALQKSPGYVSSV DSS++ DTT
Sbjct: 68  PRL-------GMLPSKLIHTYNHAINGFSASITPSELKALQKSPGYVSSVPDSSLQVDTT 127

Query: 124 HSSHFLGLNSNWGLLPISNYGSDVIIGLVDTGVWPESESFSDDGMSEIPSRWKGECESGT 183
           HSSHFLGL+S+ G+L +SNYGSDVIIG VDTGVW ESESFSD+GM EIPSRWKG+CESGT
Sbjct: 128 HSSHFLGLSSDSGVL-LSNYGSDVIIGFVDTGVWAESESFSDEGMPEIPSRWKGKCESGT 187

Query: 184 QFAASLCNKKLIGARFFNKGLIAKFPNVTISMNSTRDTIGHGTHTSATAAGNYVNTASFF 243
                 CNKKLIGARFFN+GLIAKFPNVTISMN T DT GHGTHT+ATAAG YV  ASFF
Sbjct: 188 H-----CNKKLIGARFFNRGLIAKFPNVTISMNYTNDTNGHGTHTAATAAGGYVKDASFF 247

Query: 244 GYGEGTARGVAPRARVAIYKAIWDEGNYLSDVIAAIDQAILDGVDVISLSLGLDGVPLYE 303
           GYG GTARGVAPRARVAIYKAIW+EGNY+SDVIAAIDQAILD VDV+SLSLGLDG  LYE
Sbjct: 248 GYGRGTARGVAPRARVAIYKAIWEEGNYVSDVIAAIDQAILDSVDVLSLSLGLDGALLYE 307

Query: 304 DPVAIATFAAVERGIFVATSAGNKGPQLGTVHNGAPWVLNVAAGTMDRDFGGTIILGDGV 363
           DPVAIATFAAVERGI V TSAGNKGPQLGTVHNGAPWVLNVAAGTMDRDFGGTI LG+GV
Sbjct: 308 DPVAIATFAAVERGILVTTSAGNKGPQLGTVHNGAPWVLNVAAGTMDRDFGGTIRLGNGV 367

Query: 364 SILGSSLFPVNSAMATSRLIPIVFMGGCYNLKKLKKVGYKIVVCEDKDD----SLSLQVD 423
           S+LGSSLFP+NS MATS+L P+VFMGGCYNLKKLKKVG+KIVVCEDKD+    SLSLQVD
Sbjct: 368 SVLGSSLFPLNSGMATSKL-PLVFMGGCYNLKKLKKVGHKIVVCEDKDEYNSYSLSLQVD 427

Query: 424 NVQSAKVAGGIFISNISDWDNLIPTSFPTIFLTPDRGNIVKAYMKSSSNPKARVSFHKTI 483
           NV++AKV  G+FISN+SDWDN + TSFP+IFL+P  GNI+K Y+++SSNPKARVSFHKTI
Sbjct: 428 NVETAKVGVGVFISNLSDWDNSVQTSFPSIFLSPSNGNIIKHYIRTSSNPKARVSFHKTI 487

Query: 484 FGRKPAPSVARYSSRGPSESCPFVLKPDITAPGDAILASWPQNVAATDVNSRPIYSKFNI 543
           FGRKPAPSVARYSSRGP+ESCPFVLKPDI APGDAILASWP  VAATDVNSRPIYSKFN+
Sbjct: 488 FGRKPAPSVARYSSRGPAESCPFVLKPDIMAPGDAILASWPLKVAATDVNSRPIYSKFNV 547

Query: 544 RSGTSMSCPHAAGVAALLKGAHPEWSPAAIRSAMMTTADVIDNTETQIKDLGNENRLATP 603
            SGTSMSCPHAAGVAALLK AHP WSPAAIRSAMMTTADV+DNT+T IKDLG +N+LATP
Sbjct: 548 LSGTSMSCPHAAGVAALLKAAHPRWSPAAIRSAMMTTADVVDNTQTPIKDLGEKNKLATP 607

Query: 604 LAMGSGHVNPNKAVDPGLIYDMGLQDYVNLLCALNYTKIQIQTITRSASHTCSSPSLDLN 663
           LAMGSGHVNPNKAVDPGLIYDM +QDYVN+LCALNY K QIQTITRSAS+ C +PSLDLN
Sbjct: 608 LAMGSGHVNPNKAVDPGLIYDMEIQDYVNVLCALNYNKNQIQTITRSASNNCKNPSLDLN 667

Query: 664 YPSFIVIVNASDSKTSTEISQEFKRRVTNLGEKATTYEAKLTPM--NGLRVTVKPEKLEF 723
           YPSFIVIVNASDS T  EIS+EFKR VTN+ E+ATTYEAKLTPM  NG+RVTVKP KLEF
Sbjct: 668 YPSFIVIVNASDSNTEMEISREFKRTVTNVVEEATTYEAKLTPMNGNGVRVTVKPHKLEF 727

Query: 724 KRKNHKLSFKLKIAGRRRRVKRETDVVFGYLSWVEVGGGHVARSPIVVAGGLKSKVRSH 775
           K KNHKLSF +K+      V+R+ +VVF YLSWVEVGGGHVARSPIVV   L   +RSH
Sbjct: 728 KGKNHKLSFTVKVQLGHWIVQRDPNVVFCYLSWVEVGGGHVARSPIVVT-DLNFMLRSH 769

BLAST of Sgr028602 vs. ExPASy TrEMBL
Match: A0A6J1HH07 (subtilisin-like protease SBT1.9 OS=Cucurbita moschata OX=3662 GN=LOC111462861 PE=3 SV=1)

HSP 1 Score: 1203.3 bits (3112), Expect = 0.0e+00
Identity = 622/780 (79.74%), Postives = 686/780 (87.95%), Query Frame = 0

Query: 1   MAAHNCLYFWFWFWFMAIFWVPSALTETDNYIVHMDMAAMPKPFASHHSWYSATLSSV-L 60
           MAAH  +  +FW   M IF   S LT+TDNYIVHMD+AAMPKPFA+HHSWYSATLSSV L
Sbjct: 1   MAAH--MRMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLL 60

Query: 61  DNSRLRITASSSSFS-SSKLIHSYNHAINGFSASLTPSEHEALQKSPGYVSSVRDSSVEA 120
           D S LR T SSSS S  SKLIH Y HAINGF+ASLTPS+ +AL+ SPGYVSS+RDSSV A
Sbjct: 61  DTSPLRTTTSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRA 120

Query: 121 DTTHSSHFLGLNSNWGLLPISNYGSDVIIGLVDTGVWPESESFSDDGMSEIPSRWKGECE 180
           DTTHSS+FL L+ N GLLPISNYGSDVIIG VDTGVWPESESF+DDG+S+IPSRWKGECE
Sbjct: 121 DTTHSSNFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECE 180

Query: 181 SGTQFAASLCNKKLIGARFFNKGLIAKFPNVTISMNSTRDTIGHGTHTSATAAGNYVNTA 240
           SGT F ASLCNKKLIG RFFNKGLI+KFPNVTISMNSTRDT GHGTHTS TAAG+YV  A
Sbjct: 181 SGTHFNASLCNKKLIGGRFFNKGLISKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEA 240

Query: 241 SFFGYGEGTARGVAPRARVAIYKAIWDEGNYLSDVIAAIDQAILDGVDVISLSLGLDGVP 300
           SFFGYG+GTARGVAPRARVAIYKAIW EGN +SDVIAAIDQAI DGVDVISLSLGLDGVP
Sbjct: 241 SFFGYGQGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVP 300

Query: 301 LYEDPVAIATFAAVERGIFVATSAGNKGPQLGTVHNGAPWVLNVAAGTMDRDFGGTIILG 360
           LYEDPVAIATFAA+ERGIFVATSAGNKGPQ GTVH+GAPWVLNVAAGTMDRDFGGTI L 
Sbjct: 301 LYEDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLT 360

Query: 361 DGVSILGSSLFPVNSAMATSRLIPIVFMGGCYNLKKLKKVGYKIVVCEDKDD-SLSLQVD 420
           +GVS+LGSSLFP+NSA+A S L PIVFMG C+NLKKLK+VG+KIVVCED D+ SL LQVD
Sbjct: 361 NGVSVLGSSLFPLNSAIALSPL-PIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVD 420

Query: 421 NVQSAKVAGGIFISNISDWDNLIPTSFPTIFLTPDRGNIVKAYMKSSSNPKARVSFHKTI 480
           NV+SAK+A G+FISNISDWDNLI TSFP+IFL    GN++K Y+K SSNPKARV+FHKTI
Sbjct: 421 NVESAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTI 480

Query: 481 FGRKPAPSVARYSSRGPSESCPFVLKPDITAPGDAILASWPQNVAATDVNSRPIYSKFNI 540
            G KPAPSVARYSSRGPSESCPFVLKPDI APGDAILASWPQNVAATDV SRPIYSKFN+
Sbjct: 481 IGTKPAPSVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNV 540

Query: 541 RSGTSMSCPHAAGVAALLKGAHPEWSPAAIRSAMMTTADVIDNTETQIKDLGNENRLATP 600
            SGTSM+CPHAAGVAALLKGAHP WSPAAIRSAMMTTAD++DNT+T IKDLGN+N++ATP
Sbjct: 541 LSGTSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATP 600

Query: 601 LAMGSGHVNPNKAVDPGLIYDMGLQDYVNLLCALNYTKIQIQTITRSASHTCSSPSLDLN 660
           LAMGSGHVNPNKA+DPGLIYDMG++DY NLLCALNYTK QIQTITRS S+ C    LDLN
Sbjct: 601 LAMGSGHVNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLN 660

Query: 661 YPSFIVIVNASDSKTS-TEISQEFKRRVTNLGEKATTYEAKLTPMNGLRVTVKPEKLEFK 720
           YPSFI+ VNASDS+T  TE+S+EFKRRVTN+GEK  TY AK+TPM GL VTV+P KL+FK
Sbjct: 661 YPSFIMTVNASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFK 720

Query: 721 RKNHKLSFKLKIAGRRRRVKRETDVVFGYLSWVEVGGGHVARSPIVVAGGLKSKVRSHWN 777
           RKN  LSFKLKI G    +KRE+DVVFGYL+WVEVGGGH  +SPIVVA  + + +RSHWN
Sbjct: 721 RKNQNLSFKLKIRG-HVSIKRESDVVFGYLNWVEVGGGHRVQSPIVVA--VVAGLRSHWN 774

BLAST of Sgr028602 vs. ExPASy TrEMBL
Match: A0A6J1K9W2 (subtilisin-like protease SBT1.9 OS=Cucurbita maxima OX=3661 GN=LOC111491986 PE=3 SV=1)

HSP 1 Score: 1189.5 bits (3076), Expect = 0.0e+00
Identity = 617/779 (79.20%), Postives = 676/779 (86.78%), Query Frame = 0

Query: 1   MAAHNCLYFWFWFWFMAIFWVPSALTETDNYIVHMDMAAMPKPFASHHSWYSATLSSV-L 60
           MAAH  +  +F    M I    S LTETDNYIVHMD+AAMPKPFA+HHSWYSATLSSV L
Sbjct: 1   MAAH--MRMFFCLSLMVILLARSTLTETDNYIVHMDLAAMPKPFATHHSWYSATLSSVLL 60

Query: 61  DNSRLRITASSSSFSSSKLIHSYNHAINGFSASLTPSEHEALQKSPGYVSSVRDSSVEAD 120
           D S LR T SSSS   SKLIH Y HAINGF+A+LTPS+ +AL+ SPGYVSSVRDSSV AD
Sbjct: 61  DTSPLRTTTSSSSSLPSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRAD 120

Query: 121 TTHSSHFLGLNSNWGLLPISNYGSDVIIGLVDTGVWPESESFSDDGMSEIPSRWKGECES 180
           TTHSS+FL L  N GLLPISNYGSDVIIG VDTGVWPESESF+D+ +S+IPSRWKGECES
Sbjct: 121 TTHSSNFLALTPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDEAISKIPSRWKGECES 180

Query: 181 GTQFAASLCNKKLIGARFFNKGLIAKFPNVTISMNSTRDTIGHGTHTSATAAGNYVNTAS 240
           GT F ASLCNKKLIG RFFNKGLIAKFPNVTISMNSTRDT GHGTHTS TAAG+YV  AS
Sbjct: 181 GTHFNASLCNKKLIGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEAS 240

Query: 241 FFGYGEGTARGVAPRARVAIYKAIWDEGNYLSDVIAAIDQAILDGVDVISLSLGLDGVPL 300
           FFGYG+GTARGVAPRARVAIYKAIW EGN +SDVIAAIDQAI DGVDVISLSLGLDGVPL
Sbjct: 241 FFGYGQGTARGVAPRARVAIYKAIWKEGNVVSDVIAAIDQAISDGVDVISLSLGLDGVPL 300

Query: 301 YEDPVAIATFAAVERGIFVATSAGNKGPQLGTVHNGAPWVLNVAAGTMDRDFGGTIILGD 360
           YEDPVAIATFAA+ERGIFVATSAGNKGPQ GTVH+GAPWVLNVAAGTMDRDFGGTI L +
Sbjct: 301 YEDPVAIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLNN 360

Query: 361 GVSILGSSLFPVNSAMATSRLIPIVFMGGCYNLKKLKKVGYKIVVCEDKDD-SLSLQVDN 420
           GVS+LGSSLF +NSA+  S L PIVFMG C+NLKKLK+VG+KIVVCED D+ SL LQVDN
Sbjct: 361 GVSVLGSSLFVLNSAIVLSPL-PIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDN 420

Query: 421 VQSAKVAGGIFISNISDWDNLIPTSFPTIFLTPDRGNIVKAYMKSSSNPKARVSFHKTIF 480
           VQSAK+A G+FISNISDWDNLI TSFP+IFL    GN++K Y+  SSNPKARV+FHKTI 
Sbjct: 421 VQSAKIAVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIMRSSNPKARVNFHKTII 480

Query: 481 GRKPAPSVARYSSRGPSESCPFVLKPDITAPGDAILASWPQNVAATDVNSRPIYSKFNIR 540
           G KPAPSVARYSSRGPSESCP VLKPDI APGDAILASWPQ VAATDV SRPIYSKFN+ 
Sbjct: 481 GTKPAPSVARYSSRGPSESCPLVLKPDIMAPGDAILASWPQKVAATDVYSRPIYSKFNVL 540

Query: 541 SGTSMSCPHAAGVAALLKGAHPEWSPAAIRSAMMTTADVIDNTETQIKDLGNENRLATPL 600
           SGTSM+CPHAAGVAALLKGAHP WSPAAIRSAMMTTADV+DNT+T IKDLGN+N++ATPL
Sbjct: 541 SGTSMACPHAAGVAALLKGAHPGWSPAAIRSAMMTTADVVDNTQTLIKDLGNKNKVATPL 600

Query: 601 AMGSGHVNPNKAVDPGLIYDMGLQDYVNLLCALNYTKIQIQTITRSASHTCSSPSLDLNY 660
           AMGSGHVNPNKA+DPGLIYD+G++DY NLLCALNYTK QIQTITRS S+ C  PSLDLNY
Sbjct: 601 AMGSGHVNPNKAIDPGLIYDIGIEDYTNLLCALNYTKNQIQTITRSPSNDCEKPSLDLNY 660

Query: 661 PSFIVIVNASDSKTS-TEISQEFKRRVTNLGEKATTYEAKLTPMNGLRVTVKPEKLEFKR 720
           PSFI+ VNASDS++   EIS+EFKRRVTN+GEK  TY AK+TPM GL VTV+P+ L+FKR
Sbjct: 661 PSFIITVNASDSESGRREISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPKMLKFKR 720

Query: 721 KNHKLSFKLKIAGRRRRVKRETDVVFGYLSWVEVGGGHVARSPIVVAGGLKSKVRSHWN 777
           KN  LSFKLKI G    VKRE+DVVFGYL+WVEVGGGH  +SPIVVAG     +RSHWN
Sbjct: 721 KNQNLSFKLKIRG-HVSVKRESDVVFGYLNWVEVGGGHRVQSPIVVAG-----LRSHWN 770

BLAST of Sgr028602 vs. ExPASy TrEMBL
Match: A0A5A7V589 (Subtilisin-like protease SBT1.9 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00840 PE=3 SV=1)

HSP 1 Score: 1142.5 bits (2954), Expect = 0.0e+00
Identity = 582/760 (76.58%), Postives = 648/760 (85.26%), Query Frame = 0

Query: 10  WFWFWFMAIFWVPSALTETDNYIVHMDMAAMPKPFASHHSWYSATLSSVLDNSRLRITAS 69
           +FWF    IFW+   LTET NYIVHM+ AAMPKPFAS HSWYSAT+SSVL +S    ++S
Sbjct: 6   YFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSSSSSSS 65

Query: 70  SSSFSS--SKLIHSYNHAINGFSASLTPSEHEALQKSPGYVSSVRDSSVEADTTHSSHFL 129
           SSS SS  SKLIH+YNHAI+GF ASLTPS+ EAL+ SPGY+SSV DSSV  DTTHSSHFL
Sbjct: 66  SSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFL 125

Query: 130 GLNSNWGLLPISNYGSDVIIGLVDTGVWPESESFSDDGMSEIPSRWKGECESGTQFAASL 189
           GL+SN GLLPIS YGSDVIIG VDTG+WP+SESF+DDGMSEIPSRWKGECES T F  S 
Sbjct: 126 GLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTHFNVSF 185

Query: 190 CNKKLIGARFFNKGLIAKFPNVTISMNSTRDTIGHGTHTSATAAGNYVNTASFFGYGEGT 249
           CN KLIGARFFNKGLIA+FPN TISMNSTRDTIGHGTHTS  AAG+YV  ASFFGYG GT
Sbjct: 186 CNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTIAAGSYVKEASFFGYGRGT 245

Query: 250 ARGVAPRARVAIYKAIWDEGNYLSDVIAAIDQAILDGVDVISLSLGLDGVPLYEDPVAIA 309
           ARGVAPRARVAIYKAIWDEGN +SDV+AAIDQAI DGVDVISLS+G+DG+PLY DPV+IA
Sbjct: 246 ARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGIPLYNDPVSIA 305

Query: 310 TFAAVERGIFVATSAGNKGPQLGTVHNGAPWVLNVAAGTMDRDFGGTIILGDGVSILGSS 369
           TFAAVERGIFVATSAGN GPQLGTVHNGAPWVLNVAAGTMDRDFGGTI L +GVS+LGSS
Sbjct: 306 TFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSVLGSS 365

Query: 370 LFPVNSAMATSRLIPIVFMGGCYNLKKLKKVGYKIVVCEDKDD-SLSLQVDNVQSAKVAG 429
           LFP+N+ M  S L PIVFMGGC NLKKLK++GYKIVVCED D  SL+ QVDNVQ+AKV  
Sbjct: 366 LFPLNTTMGLSPL-PIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTAKVTL 425

Query: 430 GIFISNISDWDNLIPTSFPTIFLTPDRGNIVKAYMKSSSNPKARVSFHKTIFGRKPAPSV 489
           GIFISNI DWDNLI T FP+IF+ P  GNI+K Y+  SS+PKA V+FHKTI G KPAP+V
Sbjct: 426 GIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHKTILGTKPAPTV 485

Query: 490 ARYSSRGPSESCPFVLKPDITAPGDAILASWPQNVAATDVNSRPIYSKFNIRSGTSMSCP 549
           ARYSSRGPS+SCPFVLKPDI APGDAILASWPQN+ A DVNS PIY+KFN+ SGTSMSCP
Sbjct: 486 ARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVISGTSMSCP 545

Query: 550 HAAGVAALLKGAHPEWSPAAIRSAMMTTADVIDNTETQIKDLGNENRLATPLAMGSGHVN 609
           HAAGVAALLKGAHP+WSPAAIRSAMMTTADV+DNT+T IKD+GN N+ ATPLAMGSGHVN
Sbjct: 546 HAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPLAMGSGHVN 605

Query: 610 PNKAVDPGLIYDMGLQDYVNLLCALNYTKIQIQTITRSASHTCSSPSLDLNYPSFIVIVN 669
           PNKAVDP LIYD+G+QDYVN+LCALNYT+ QI+ ITRS S+ C +PSLDLNYPSFI+I N
Sbjct: 606 PNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIIIAN 665

Query: 670 ASDSKT-STEISQEFKRRVTNLGEKATTYEAKLTPMNGLRVTVKPEKLEFKRKNHKLSFK 729
           +SDSKT   +I  EFKR +T +GE   TYEAKL  M G +V VKP  LEFKRKN KLSF+
Sbjct: 666 SSDSKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRILEFKRKNQKLSFE 725

Query: 730 LKIAGRRRRVKRETDVVFGYLSWVEVGGGHVARSPIVVAG 766
           LKIAG       E++VVFGYLSW EVGG H+ +SPIVVAG
Sbjct: 726 LKIAGS----AEESNVVFGYLSWAEVGGRHIVQSPIVVAG 760

BLAST of Sgr028602 vs. ExPASy TrEMBL
Match: A0A1S3BBC3 (subtilisin-like protease SBT1.9 OS=Cucumis melo OX=3656 GN=LOC103487840 PE=3 SV=1)

HSP 1 Score: 1142.5 bits (2954), Expect = 0.0e+00
Identity = 582/760 (76.58%), Postives = 648/760 (85.26%), Query Frame = 0

Query: 10  WFWFWFMAIFWVPSALTETDNYIVHMDMAAMPKPFASHHSWYSATLSSVLDNSRLRITAS 69
           +FWF    IFW+   LTET NYIVHM+ AAMPKPFAS HSWYSAT+SSVL +S    ++S
Sbjct: 6   YFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSSSSSSS 65

Query: 70  SSSFSS--SKLIHSYNHAINGFSASLTPSEHEALQKSPGYVSSVRDSSVEADTTHSSHFL 129
           SSS SS  SKLIH+YNHAI+GF ASLTPS+ EAL+ SPGY+SSV DSSV  DTTHSSHFL
Sbjct: 66  SSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTHSSHFL 125

Query: 130 GLNSNWGLLPISNYGSDVIIGLVDTGVWPESESFSDDGMSEIPSRWKGECESGTQFAASL 189
           GL+SN GLLPIS YGSDVIIG VDTG+WP+SESF+DDGMSEIPSRWKGECES T F  S 
Sbjct: 126 GLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTHFNVSF 185

Query: 190 CNKKLIGARFFNKGLIAKFPNVTISMNSTRDTIGHGTHTSATAAGNYVNTASFFGYGEGT 249
           CN KLIGARFFNKGLIA+FPN TISMNSTRDTIGHGTHTS  AAG+YV  ASFFGYG GT
Sbjct: 186 CNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTIAAGSYVKEASFFGYGRGT 245

Query: 250 ARGVAPRARVAIYKAIWDEGNYLSDVIAAIDQAILDGVDVISLSLGLDGVPLYEDPVAIA 309
           ARGVAPRARVAIYKAIWDEGN +SDV+AAIDQAI DGVDVISLS+G+DG+PLY DPV+IA
Sbjct: 246 ARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGIPLYNDPVSIA 305

Query: 310 TFAAVERGIFVATSAGNKGPQLGTVHNGAPWVLNVAAGTMDRDFGGTIILGDGVSILGSS 369
           TFAAVERGIFVATSAGN GPQLGTVHNGAPWVLNVAAGTMDRDFGGTI L +GVS+LGSS
Sbjct: 306 TFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSVLGSS 365

Query: 370 LFPVNSAMATSRLIPIVFMGGCYNLKKLKKVGYKIVVCEDKDD-SLSLQVDNVQSAKVAG 429
           LFP+N+ M  S L PIVFMGGC NLKKLK++GYKIVVCED D  SL+ QVDNVQ+AKV  
Sbjct: 366 LFPLNTTMGLSPL-PIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTAKVTL 425

Query: 430 GIFISNISDWDNLIPTSFPTIFLTPDRGNIVKAYMKSSSNPKARVSFHKTIFGRKPAPSV 489
           GIFISNI DWDNLI T FP+IF+ P  GNI+K Y+  SS+PKA V+FHKTI G KPAP+V
Sbjct: 426 GIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHKTILGTKPAPTV 485

Query: 490 ARYSSRGPSESCPFVLKPDITAPGDAILASWPQNVAATDVNSRPIYSKFNIRSGTSMSCP 549
           ARYSSRGPS+SCPFVLKPDI APGDAILASWPQN+ A DVNS PIY+KFN+ SGTSMSCP
Sbjct: 486 ARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVISGTSMSCP 545

Query: 550 HAAGVAALLKGAHPEWSPAAIRSAMMTTADVIDNTETQIKDLGNENRLATPLAMGSGHVN 609
           HAAGVAALLKGAHP+WSPAAIRSAMMTTADV+DNT+T IKD+GN N+ ATPLAMGSGHVN
Sbjct: 546 HAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPLAMGSGHVN 605

Query: 610 PNKAVDPGLIYDMGLQDYVNLLCALNYTKIQIQTITRSASHTCSSPSLDLNYPSFIVIVN 669
           PNKAVDP LIYD+G+QDYVN+LCALNYT+ QI+ ITRS S+ C +PSLDLNYPSFI+I N
Sbjct: 606 PNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFIIIAN 665

Query: 670 ASDSKT-STEISQEFKRRVTNLGEKATTYEAKLTPMNGLRVTVKPEKLEFKRKNHKLSFK 729
           +SDSKT   +I  EFKR +T +GE   TYEAKL  M G +V VKP  LEFKRKN KLSF+
Sbjct: 666 SSDSKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRILEFKRKNQKLSFE 725

Query: 730 LKIAGRRRRVKRETDVVFGYLSWVEVGGGHVARSPIVVAG 766
           LKIAG       E++VVFGYLSW EVGG H+ +SPIVVAG
Sbjct: 726 LKIAGS----AEESNVVFGYLSWAEVGGRHIVQSPIVVAG 760

BLAST of Sgr028602 vs. TAIR 10
Match: AT5G67090.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 593.2 bits (1528), Expect = 3.0e-169
Identity = 339/767 (44.20%), Postives = 468/767 (61.02%), Query Frame = 0

Query: 19  FWVPSALTETDNYIVHMDMAAMPKPFASHHSWYSATLSSVLDNSRLRITASSSSFSSSKL 78
           F+V     ET  YI+HMD++A P PF+ H SW+S TL+SV+ N +             K+
Sbjct: 13  FFVAIVTAETSPYIIHMDLSAKPLPFSDHRSWFSTTLTSVITNRK------------PKI 72

Query: 79  IHSYNHAINGFSASLTPSEHEALQKSPGYVSSVRDSSVEADTTHSSHFLGLNSNWGLLPI 138
           I++Y  +++GFSA LT SE + L+  PGYVS  +D  V+  TT S  F+GLNS  G  P+
Sbjct: 73  IYAYTDSVHGFSAVLTNSELQRLKHKPGYVSFTKDLPVKLHTTFSPKFIGLNSTSGTWPV 132

Query: 139 SNYGSDVIIGLVDTGVWPESESFSDDGMSEIPSRWKGECESGTQFAASLCNKKLIGARFF 198
           SNYG+ ++IG++DTG+WP+S SF DDG+  +PS+WKG CE     ++SLCNKKLIGA+ F
Sbjct: 133 SNYGAGIVIGIIDTGIWPDSPSFHDDGVGSVPSKWKGACEFN---SSSLCNKKLIGAKVF 192

Query: 199 NKGLIAKFPNVTIS----MNSTRDTIGHGTHTSATAAGNYVNTASFFGYGEGTARGVAPR 258
           NKGL A  P++  +     +S  DTIGHGTH +A AAGN+V  AS+F Y +GTA G+AP 
Sbjct: 193 NKGLFANNPDLRETKIGQYSSPYDTIGHGTHVAAIAAGNHVKNASYFSYAQGTASGIAPH 252

Query: 259 ARVAIYKAIWDEGNYLSDVIAAIDQAILDGVDVISLSLGL--------DGVPLYEDPVAI 318
           A +AIYKA W+EG Y SDVIAAIDQAI DGV VISLSLGL        DG  L  DP+A+
Sbjct: 253 AHLAIYKAAWEEGIYSSDVIAAIDQAIRDGVHVISLSLGLSFEDDDDNDGFGLENDPIAV 312

Query: 319 ATFAAVERGIFVATSAGNKGPQLGTVHNGAPWVLNVAAGTMDRDFGGTIILGDGVSILGS 378
           A+FAA+++G+FV TS GN GP   ++ NGAPW++ V AGT+ R F GT+  G+ VS    
Sbjct: 313 ASFAAIQKGVFVVTSGGNDGPYYWSLINGAPWIMTVGAGTIGRQFQGTLTFGNRVSFSFP 372

Query: 379 SLFPVNSAMATSRLIPIVFM--GGCYNLKKLKKVGYKIVVCEDKDDSLSLQVDNVQSAKV 438
           SLFP       S   P+ ++  G   N    K +  +IVVC +++ ++  ++  ++S   
Sbjct: 373 SLFP---GEFPSVQFPVTYIESGSVEN----KTLANRIVVC-NENINIGSKLHQIRSTGA 432

Query: 439 AGGIFISN-ISDWDNLIPTSFPTIFLTPDRGNIVKAYMKSS-SNPKARVSFHKTIFGRKP 498
           A  + I++ + +  + I   FP  F+       +++Y  S+ +N  A++ F KT+ G KP
Sbjct: 433 AAVVLITDKLLEEQDTIKFQFPVAFIGSKHRETIESYASSNKNNATAKLEFRKTVIGTKP 492

Query: 499 APSVARYSSRGPSESCPFVLKPDITAPGDAILASWPQNVAATDVNSRPIYSKFNIRSGTS 558
           AP V  YSSRGP  S P +LKPDI APG  IL++WP     T   + P++S FN+ +GTS
Sbjct: 493 APEVGTYSSRGPFTSFPQILKPDILAPGTLILSAWPSVEQITGTRALPLFSGFNLLTGTS 552

Query: 559 MSCPHAAGVAALLKGAHPEWSPAAIRSAMMTTADVIDNTETQIKDLGNENRLATPLAMGS 618
           M+ PH AGVAAL+K  HP WSP+AI+SA+MTTA  +DN                PLA+G+
Sbjct: 553 MAAPHVAGVAALIKQVHPNWSPSAIKSAIMTTALTLDN----------------PLAVGA 612

Query: 619 GHVNPNKAVDPGLIYDMGLQDYVNLLC-ALNYTKIQIQTITRS-ASHTCSSPSLDLNYPS 678
           GHV+ NK ++PGLIYD   QD++N LC     ++  I  ITRS  S  C  PS  LNYPS
Sbjct: 613 GHVSTNKVLNPGLIYDTTPQDFINFLCHEAKQSRKLINIITRSNISDACKKPSPYLNYPS 672

Query: 679 FIVIVNASDSKTSTEISQEFKRRVTNLGEKATTYEAKLTPMNGLRVTVKPEKLEFKRKNH 738
            I    +  S         FKR +TN+GE   +Y  ++  + GL V V+P+KL F  KN 
Sbjct: 673 IIAYFTSDQSSPKI-----FKRTLTNVGEAKRSYIVRVRGLKGLNVVVEPKKLMFSEKNE 732

Query: 739 KLSFKLKIAGRRRRVKRETDVVFGYLSWVEVGGGHVARSPIVVAGGL 768
           KLS+ +++   R     + +VV+G +SWV+        S  VVA  L
Sbjct: 733 KLSYTVRLESPR---GLQENVVYGLVSWVDEDEAEFEVSCSVVATSL 732

BLAST of Sgr028602 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 535.4 bits (1378), Expect = 7.5e-152
Identity = 320/764 (41.88%), Postives = 448/764 (58.64%), Query Frame = 0

Query: 21  VPSALTETDNYIVHMDMAAMPKPFASHHSWYSATLSSVLDNSRLRITASSSSFSSSKLIH 80
           V S+ ++   YIVHM  + MP  F  H +WY ++L S+ D              S++L++
Sbjct: 22  VSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISD--------------SAELLY 81

Query: 81  SYNHAINGFSASLTPSEHEALQKSPGYVSSVRDSSVEADTTHSSHFLGLNSNWG-LLPIS 140
           +Y +AI+GFS  LT  E ++L   PG +S + +   E  TT +  FLGL+ +   L P +
Sbjct: 82  TYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEA 141

Query: 141 NYGSDVIIGLVDTGVWPESESFSDDGMSEIPSRWKGECESGTQFAASLCNKKLIGARFFN 200
              SDV++G++DTGVWPES+S+SD+G   IPS WKG CE+GT F ASLCN+KLIGARFF 
Sbjct: 142 GSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFA 201

Query: 201 KGLIAKFPNVTIS--MNSTRDTIGHGTHTSATAAGNYVNTASFFGYGEGTARGVAPRARV 260
           +G  +    +  S    S RD  GHGTHTS+TAAG+ V  AS  GY  GTARG+APRARV
Sbjct: 202 RGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARV 261

Query: 261 AIYKAIWDEGNYLSDVIAAIDQAILDGVDVISLSLGLDGVPLYEDPVAIATFAAVERGIF 320
           A+YK  W  G + SD++AAID+AI D V+V+S+SLG      Y D VAI  FAA+ERGI 
Sbjct: 262 AVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGIL 321

Query: 321 VATSAGNKGPQLGTVHNGAPWVLNVAAGTMDRDFGGTIILGDGVSILGSSLFPVNSAMAT 380
           V+ SAGN GP   ++ N APW+  V AGT+DRDF    ILG+G +  G SLF        
Sbjct: 322 VSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLF--KGEALP 381

Query: 381 SRLIPIVFMGGCYNLKK----------LKKVGYKIVVCEDKDDSLSLQVDNVQSAKVAGG 440
            +L+P ++ G   N              +KV  KIV+C+   ++   + D V++A   G 
Sbjct: 382 DKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGM 441

Query: 441 IFISNISDWDNLIPTS--FPTIFLTPDRGNIVKAYMKSSSNPKARVSFHKTIFGRKPAPS 500
           I  +  ++ + L+  +   P   +    G+I++ Y+ +  NP A +S   T+ G KP+P 
Sbjct: 442 ILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPV 501

Query: 501 VARYSSRGPSESCPFVLKPDITAPGDAILASWPQNVAATDVNSRPIYSKFNIRSGTSMSC 560
           VA +SSRGP+   P +LKPD+ APG  ILA+W      T + S     +FNI SGTSMSC
Sbjct: 502 VAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSC 561

Query: 561 PHAAGVAALLKGAHPEWSPAAIRSAMMTTADVIDNTETQIKDLGNENRLATPLAMGSGHV 620
           PH +G+AALLK  HPEWSPAAIRSA+MTTA         + D+    + +TP   G+GHV
Sbjct: 562 PHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIAT-GKPSTPFDHGAGHV 621

Query: 621 NPNKAVDPGLIYDMGLQDYVNLLCALNYTKIQIQTITRSASHTC----SSPSLDLNYPSF 680
           +P  A +PGLIYD+  +DY+  LCALNYT  QI++++R  ++TC    S    DLNYPSF
Sbjct: 622 SPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSR-RNYTCDPSKSYSVADLNYPSF 681

Query: 681 IVIVNASDSKTSTEISQEFKRRVTNLGEKATTYEAKLT-PMNGLRVTVKPEKLEFKRKNH 740
            V V+          + ++ R VT++G  A TY  K+T    G++++V+P  L FK  N 
Sbjct: 682 AVNVDGVG-------AYKYTRTVTSVG-GAGTYSVKVTSETTGVKISVEPAVLNFKEANE 741

Query: 741 KLSFKLKIAGRRRRVKRETDVVFGYLSWVEVGGGHVARSPIVVA 765
           K S+ +       +        FG + W +  G HV  SP+ ++
Sbjct: 742 KKSYTVTFTVDSSKPSGSNS--FGSIEWSD--GKHVVGSPVAIS 755

BLAST of Sgr028602 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 529.3 bits (1362), Expect = 5.4e-150
Identity = 326/788 (41.37%), Postives = 457/788 (57.99%), Query Frame = 0

Query: 9   FWFWFWFMAIFWVPSALTETDN---YIVHMDMAAMPKPFASHHSWYSATLSSVLDNSRLR 68
           F+F+F+F+     PS+   + N   YIVH+D  A P  F +H  WY+++L+S        
Sbjct: 3   FFFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLAS-------- 62

Query: 69  ITASSSSFSSSKLIHSYNHAINGFSASLTPSEHEALQKSPGYVSSVRDSSVEADTTHSSH 128
           +T+S  S     +IH+Y+   +GFSA LT  +   L   P  +S + +      TT S  
Sbjct: 63  LTSSPPS-----IIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPE 122

Query: 129 FLGLNS--NWGLLPISNYGSDVIIGLVDTGVWPESESFSDDGMSEIPSRWKGECESGTQF 188
           FLGL S    GLL  S++GSD++IG++DTGVWPE  SF D G+  +P +WKG+C +   F
Sbjct: 123 FLGLRSTDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDF 182

Query: 189 AASLCNKKLIGARFFNKGLIAK--FPNVTISMNSTRDTIGHGTHTSATAAGNYVNTASFF 248
             S CN+KL+GARFF  G  A     N T    S RD+ GHGTHT++ +AG YV  AS  
Sbjct: 183 PESACNRKLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTL 242

Query: 249 GYGEGTARGVAPRARVAIYKAIWDEGNYLSDVIAAIDQAILDGVDVISLSLGLDGVPLYE 308
           GY  G A G+AP+AR+A YK  W+ G Y SD++AA D A+ DGVDVISLS+G   VP Y 
Sbjct: 243 GYAHGVAAGMAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYL 302

Query: 309 DPVAIATFAAVERGIFVATSAGNKGPQLGTVHNGAPWVLNVAAGTMDRDFGGTIILGDGV 368
           D +AI  F A++RGIFV+ SAGN GP   TV N APW+  V AGT+DRDF   + LG+G 
Sbjct: 303 DAIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGK 362

Query: 369 SILGSSLFPVNSAMATSRLIPIVF----MGGCYNLKKL--------KKVGYKIVVCEDKD 428
            I G S++     +   R+ P+V+    +GG      L          V  KIV+C+   
Sbjct: 363 MISGVSVYG-GPGLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGI 422

Query: 429 DSLSLQVDNVQSAKVAGGIFISNISDWDNLIPTS--FPTIFLTPDRGNIVKAYM------ 488
           +S + + + V+     G I  + + D + L+      P   +    G+ ++ Y+      
Sbjct: 423 NSRATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKS 482

Query: 489 KSSSNPKARVSFHKTIFGRKPAPSVARYSSRGPSESCPFVLKPDITAPGDAILASWPQNV 548
           +SS +P A + F  T  G +PAP VA +S+RGP+   P +LKPD+ APG  ILA+WP  +
Sbjct: 483 RSSKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRI 542

Query: 549 AATDVNSRPIYSKFNIRSGTSMSCPHAAGVAALLKGAHPEWSPAAIRSAMMTTADVIDNT 608
             + V S    ++FNI SGTSM+CPH +G+AALLK AHP+WSPAAIRSA++TTA  +DN+
Sbjct: 543 GPSGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNS 602

Query: 609 ETQIKDLGNENRLATPLAMGSGHVNPNKAVDPGLIYDMGLQDYVNLLCALNYTKIQIQTI 668
              + D    N  ++ +  GSGHV+P KA+DPGL+YD+   DY+N LC  NYT+  I TI
Sbjct: 603 GEPMMDESTGN-TSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTI 662

Query: 669 TRSASHTCSSPSL----DLNYPSF-IVIVNASDSKTSTEISQEFKRRVTNLGEKATTYEA 728
           TR  +    +       +LNYPSF +V     +SK ST     F R VTN+G+  + YE 
Sbjct: 663 TRRQADCDGARRAGHVGNLNYPSFSVVFQQYGESKMST----HFIRTVTNVGDSDSVYEI 722

Query: 729 KLTPMNGLRVTVKPEKLEFKRKNHKLSFKLKIAGRRRRVK-RETDVVFGYLSWVEVGGGH 764
           K+ P  G  VTV+PEKL F+R   KLSF +++     ++    T+V  G++ W +  G  
Sbjct: 723 KIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSD--GKR 769

BLAST of Sgr028602 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 526.2 bits (1354), Expect = 4.6e-149
Identity = 315/757 (41.61%), Postives = 434/757 (57.33%), Query Frame = 0

Query: 26  TETDNYIVHMDMAAMPKPFASHHSWYSATLSSVLDNSRLRITASSSSFSSSKLIHSYNHA 85
           T    YI+ ++ +  P+ F +HH WY++ L+                 S S L+++Y  +
Sbjct: 25  TAKKTYIIRVNHSDKPESFLTHHDWYTSQLN-----------------SESSLLYTYTTS 84

Query: 86  INGFSASLTPSEHEALQKSPGYVSSV-RDSSVEADTTHSSHFLGLNSNWGLLPISNYGSD 145
            +GFSA L  +E ++L  S   +  +  D      TT +  FLGLNS +G+  + +  + 
Sbjct: 85  FHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNG 144

Query: 146 VIIGLVDTGVWPESESFSDDGMSEIPSRWKGECESGTQFAASLCNKKLIGARFFNKGLI- 205
           VIIG++DTGVWPES SF D  M EIPS+WKGECESG+ F + LCNKKLIGAR F+KG   
Sbjct: 145 VIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQM 204

Query: 206 ---AKFPNVTISMNSTRDTIGHGTHTSATAAGNYVNTASFFGYGEGTARGVAPRARVAIY 265
                F +   S+ S RD  GHGTHTS TAAG+ V  ASF GY  GTARG+A RARVA Y
Sbjct: 205 ASGGGFSSKRESV-SPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATY 264

Query: 266 KAIWDEGNYLSDVIAAIDQAILDGVDVISLSLGLDGVPLYEDPVAIATFAAVERGIFVAT 325
           K  W  G + SD++AA+D+AILDGVDV+SLSLG    P Y D +AI  F+A+ERG+FV+ 
Sbjct: 265 KVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSC 324

Query: 326 SAGNKGPQLGTVHNGAPWVLNVAAGTMDRDFGGTIILGDGVSILGSSLFPVNSAMATSRL 385
           SAGN GP   +V N APWV+ V AGT+DRDF     LG+G  + G SL+     M T  L
Sbjct: 325 SAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYS-GVGMGTKPL 384

Query: 386 IPIVFMGGCYNLKKLKKVGY--------KIVVCEDKDDSLSLQVDNVQSAKVAGG---IF 445
             +V+  G  +   L   G         KIVVC   D  ++ +V+     + AGG   I 
Sbjct: 385 -ELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVC---DRGVNARVEKGAVVRDAGGLGMIM 444

Query: 446 ISNISDWDNLIPTS--FPTIFLTPDRGNIVKAYMKSSSNPKARVSFHKTIFGRKPAPSVA 505
            +  +  + L+  S   P I +    G++++ Y+KS S P A + F  T+   KP+P VA
Sbjct: 445 ANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVA 504

Query: 506 RYSSRGPSESCPFVLKPDITAPGDAILASWPQNVAATDVNSRPIYSKFNIRSGTSMSCPH 565
            +SSRGP+   P +LKPD+  PG  ILA W   +  T ++     ++FNI SGTSMSCPH
Sbjct: 505 AFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPH 564

Query: 566 AAGVAALLKGAHPEWSPAAIRSAMMTTADVIDNTETQIKDLGNENRLATPLAMGSGHVNP 625
            +G+A LLK AHPEWSP+AI+SA+MTTA V+DNT   + D   +N L+ P A GSGHV+P
Sbjct: 565 ISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAA-DNSLSNPYAHGSGHVDP 624

Query: 626 NKAVDPGLIYDMGLQDYVNLLCALNYTKIQIQTITRSASHTCSSPSLD---LNYPSFIVI 685
            KA+ PGL+YD+  ++Y+  LC+L+YT   I  I +  S  CS    D   LNYPSF V+
Sbjct: 625 QKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSVL 684

Query: 686 VNASDSKTSTEISQEFKRRVTNLGEKATTYEAKLTPMNGLRVTVKPEKLEFKRKNHKLSF 745
                     +    + R VTN+G  ++ Y+  +     + ++VKP KL FK    K  +
Sbjct: 685 FGG-------KRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRY 744

Query: 746 KLKIAGRRRRVKRETDVVFGYLSWVEVGGGHVARSPI 762
            +     ++ V       FG ++W      H  RSP+
Sbjct: 745 TVTFVS-KKGVSMTNKAEFGSITW--SNPQHEVRSPV 747

BLAST of Sgr028602 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 526.2 bits (1354), Expect = 4.6e-149
Identity = 319/758 (42.08%), Postives = 435/758 (57.39%), Query Frame = 0

Query: 31  YIVHMDMAAMPKPFASHHSWYSATLSSVLDNSRLRITASSSSFSSSKLIHSYNHAINGFS 90
           +I  +D  +MP  F +H+ WYS   +                   S+++H Y+   +GFS
Sbjct: 28  FIFRIDGGSMPSIFPTHYHWYSTEFA-----------------EESRIVHVYHTVFHGFS 87

Query: 91  ASLTPSEHEALQKSPGYVSSVRDSSVEADTTHSSHFLGLNSNWGLLPISNYGSDVIIGLV 150
           A +TP E + L+  P  ++   D   E  TT S  FLGL +  GL   S+YGSDVIIG+ 
Sbjct: 88  AVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYGSDVIIGVF 147

Query: 151 DTGVWPESESFSDDGMSEIPSRWKGECESGTQFAASLCNKKLIGARFFNKGLIAKF---P 210
           DTG+WPE  SFSD  +  IP RW+G CESG +F+   CN+K+IGARFF KG  A      
Sbjct: 148 DTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQAAVIGGI 207

Query: 211 NVTISMNSTRDTIGHGTHTSATAAGNYVNTASFFGYGEGTARGVAPRARVAIYKAIW-DE 270
           N T+   S RD  GHGTHTS+TAAG +   AS  GY  G A+GVAP+AR+A YK  W D 
Sbjct: 208 NKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYKVCWKDS 267

Query: 271 GNYLSDVIAAIDQAILDGVDVISLSL-GLDGV--PLYEDPVAIATFAAVERGIFVATSAG 330
           G   SD++AA D A+ DGVDVIS+S+ G DG+  P Y DP+AI ++ A  +GIFV++SAG
Sbjct: 268 GCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGIFVSSSAG 327

Query: 331 NKGPQLGTVHNGAPWVLNVAAGTMDRDFGGTIILGDGVSILGSSLF---PVNSAMATSRL 390
           N+GP   +V N APWV  V A T+DR+F    ILGDG  + G SL+   P+N      R+
Sbjct: 328 NEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVPLN-----GRM 387

Query: 391 IPIVFMGG---------CYNLKKLKKVGYKIVVCEDKDDSLSLQVDNVQSAKVAGGIFIS 450
            P+V+ G            N    K+V  KIV+C+        +   V+ A   G I  +
Sbjct: 388 FPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILAN 447

Query: 451 NISDWDNLIPTS--FPTIFLTPDRGNIVKAYMKSSSNPKARVSFHKTIFGRKPAPSVARY 510
             S+ + L+  +   P   +  + G+ +KAY  S  NP A + F  TI G KPAP +A +
Sbjct: 448 GASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIASF 507

Query: 511 SSRGPSESCPFVLKPDITAPGDAILASWPQNVAATDVNSRPIYSKFNIRSGTSMSCPHAA 570
           S RGP+   P +LKPD+ APG  ILA+W   V  T + S P  ++FNI SGTSM+CPH +
Sbjct: 508 SGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVS 567

Query: 571 GVAALLKGAHPEWSPAAIRSAMMTTADVIDNTETQIKDLGNENRLATPLAMGSGHVNPNK 630
           G AALLK AHP+WSPA IRSAMMTT +++DN+   + D  +  + ATP   GSGH+N  +
Sbjct: 568 GAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLID-ESTGKSATPYDYGSGHLNLGR 627

Query: 631 AVDPGLIYDMGLQDYVNLLCALNYTKIQIQTITRSASH--TCSSPSL-DLNYPSFIVIVN 690
           A++PGL+YD+   DY+  LC++ Y    IQ ITR+     T   PS  +LNYPS   +  
Sbjct: 628 AMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVF- 687

Query: 691 ASDSKTSTEISQEFKRRVTNLGEKATTYEAKLTPMNGLRVTVKPEKLEFKRKNHKLSFKL 750
              +     +S+   R  TN+G+    Y A++    G+ VTVKP +L F     + S+ +
Sbjct: 688 --PTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAV 747

Query: 751 KI-AGRRRRVKRETDVVFGYLSWVEVGGGHVARSPIVV 764
            +    R  V  ET  VFG ++W + GG HV RSPIVV
Sbjct: 748 TVTVNTRNVVLGETGAVFGSVTWFD-GGKHVVRSPIVV 758