Sgr027986 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr027986
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionMalic enzyme
Locationtig00153056: 2175690 .. 2176129 (+)
RNA-Seq ExpressionSgr027986
SyntenySgr027986
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAGGCTGAGGGTACACTACAAGATGATGATCAGTATTCTGGTGCTAGCACAGGCCGGTTCTCATTTCCTGTATTGTTTCGATTATGATGTTATCATTTTATGCATATTTTTTTCGAAATTTTATGGTGGCTAGTGTAGAATATATATAACTGCTGGTCTTTTTGATAATATTGTTTGTTTAGAATGTGATGGTGTAACATCGATTGGCAAAATTACACAGTGGCAGTTTAGTTCTAAAAGCTACGTTTTGTCGCGCAACGTCATGGACCATGATCATCAAAAACTCACAATGCAGTGTCATCTTTTCTTTAGGGAACAGCAGGAGTTGCAATTGCCAGAGCTTTAGGTGCTGTGAGGACACAGGGGAGGCTGATGATTGACTTCCCAAAGCAAAAGATTGTTGTTGCTGGTGCTGGTGCTGGTGCTGGAAGATAG

mRNA sequence

ATGAAGAGGCTGAGGGTACACTACAAGATGATGATCAGTATTCTGGTGCTAGCACAGGCCGAATGTGATGGTGTAACATCGATTGGCAAAATTACACAGTGGCAGTTTAGTTCTAAAAGCTACGTTTTGTCGCGCAACGGAACAGCAGGAGTTGCAATTGCCAGAGCTTTAGGTGCTGTGAGGACACAGGGGAGGCTGATGATTGACTTCCCAAAGCAAAAGATTGTTGTTGCTGGTGCTGGTGCTGGTGCTGGAAGATAG

Coding sequence (CDS)

ATGAAGAGGCTGAGGGTACACTACAAGATGATGATCAGTATTCTGGTGCTAGCACAGGCCGAATGTGATGGTGTAACATCGATTGGCAAAATTACACAGTGGCAGTTTAGTTCTAAAAGCTACGTTTTGTCGCGCAACGGAACAGCAGGAGTTGCAATTGCCAGAGCTTTAGGTGCTGTGAGGACACAGGGGAGGCTGATGATTGACTTCCCAAAGCAAAAGATTGTTGTTGCTGGTGCTGGTGCTGGTGCTGGAAGATAG

Protein sequence

MKRLRVHYKMMISILVLAQAECDGVTSIGKITQWQFSSKSYVLSRNGTAGVAIARALGAVRTQGRLMIDFPKQKIVVAGAGAGAGR
Homology
[Close]
BLAST of Sgr027986 vs. NCBI nr
Match: XP_021615290.1 (NAD-dependent malic enzyme 62 kDa isoform, mitochondrial-like isoform X3 [Manihot esculenta])

HSP 1 Score: 63.5 bits (153), Expect = 9.9e-07
Identity = 38/50 (76.00%), Postives = 39/50 (78.00%), Query Frame = 0 

Query: 37 SSKSYVLSRNGTAGVAIARALGAVRTQGRLMIDFPKQKIVVAGAG-AGAG 86
          SS   +LS  GTAGVAIA  LGAVR QGR MIDFPKQKIVVAGAG AG G
Sbjct: 4  SSLFSLLSVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIG 53

[Close]
BLAST of Sgr027986 vs. NCBI nr
Match: XP_030494112.1 (NAD-dependent malic enzyme 62 kDa isoform, mitochondrial-like [Cannabis sativa] >KAF4356736.1 hypothetical protein F8388_010958 [Cannabis sativa] >KAF4396169.1 hypothetical protein G4B88_020806 [Cannabis sativa])

HSP 1 Score: 61.2 bits (147), Expect = 4.9e-06
Identity = 34/40 (85.00%), Postives = 34/40 (85.00%), Query Frame = 0 

Query: 47  GTAGVAIARALGAVRTQGRLMIDFPKQKIVVAGAG-AGAG 86
           GTAGVAIA  LGAVR QGR MIDFPKQKIVVAGAG AG G
Sbjct: 327 GTAGVAIAGLLGAVRAQGRAMIDFPKQKIVVAGAGSAGIG 366

[Close]
BLAST of Sgr027986 vs. NCBI nr
Match: KAF6170155.1 (hypothetical protein GIB67_038688 [Kingdonia uniflora])

HSP 1 Score: 61.2 bits (147), Expect = 4.9e-06
Identity = 41/75 (54.67%), Postives = 50/75 (66.67%), Query Frame = 0 

Query: 15  LVLAQAECDG---VTSIGKITQWQFSSKSYVLSRNGTAGVAIARALGAVRTQGRLMIDFP 74
           L L +   DG   +T I +  +  F+   +V+ + GTAGVAIA  LGAVR QG+ MIDFP
Sbjct: 255 LGLQEHRLDGEEYITVIDEFMEAVFTRWPHVIVQ-GTAGVAIAGLLGAVRAQGKSMIDFP 314

Query: 75  KQKIVVAGAG-AGAG 86
           KQKIVVAGAG AG G
Sbjct: 315 KQKIVVAGAGSAGIG 328

[Close]
BLAST of Sgr027986 vs. NCBI nr
Match: XP_030493995.1 (NAD-dependent malic enzyme 62 kDa isoform, mitochondrial [Cannabis sativa] >KAF4362809.1 hypothetical protein F8388_022466 [Cannabis sativa])

HSP 1 Score: 61.2 bits (147), Expect = 4.9e-06
Identity = 34/40 (85.00%), Postives = 34/40 (85.00%), Query Frame = 0 

Query: 47  GTAGVAIARALGAVRTQGRLMIDFPKQKIVVAGAG-AGAG 86
           GTAGVAIA  LGAVR QGR MIDFPKQKIVVAGAG AG G
Sbjct: 327 GTAGVAIAGLLGAVRAQGRTMIDFPKQKIVVAGAGSAGIG 366

[Close]
BLAST of Sgr027986 vs. NCBI nr
Match: OEL34676.1 (NAD-dependent malic enzyme 1, mitochondrial [Dichanthelium oligosanthes])

HSP 1 Score: 60.8 bits (146), Expect = 6.4e-06
Identity = 44/96 (45.83%), Postives = 55/96 (57.29%), Query Frame = 0 

Query: 1   MKRLRVHYKMMISILVLAQAECDGVTS--IGKITQWQFSSKS--------YVLSRNGTAG 60
           ++R R  Y+M    + ++ + C  V    I  +  +   SKS         +L   GTAG
Sbjct: 295 LQRYRKTYRMFNDDVQVSPSLCKFVLKMFIRSLRLYDICSKSECIALEMTVLLCTKGTAG 354

Query: 61  VAIARALGAVRTQGRLMIDFPKQKIVVAGAG-AGAG 86
           VAIA  LGAVR QGR MIDFPKQ+IVVAGAG AG G
Sbjct: 355 VAIAGLLGAVRAQGRPMIDFPKQRIVVAGAGSAGIG 390

[Close]
BLAST of Sgr027986 vs. ExPASy Swiss-Prot
Match: P37224 (NAD-dependent malic enzyme 65 kDa isoform, mitochondrial OS=Amaranthus hypochondriacus OX=28502 PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 1.1e-08
Identity = 30/52 (57.69%), Postives = 40/52 (76.92%), Query Frame = 0 

Query: 31  ITQWQFSSKSYVLSRNGTAGVAIARALGAVRTQGRLMIDFPKQKIVVAGAGA 83
           + +++   +++ +   GT+GVAIA  LGAVR QGR MIDFPKQKIVVAGAG+
Sbjct: 296 LQRYRHKYRTFNVDVQGTSGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGS 347

[Close]
BLAST of Sgr027986 vs. ExPASy Swiss-Prot
Match: Q9SIU0 (NAD-dependent malic enzyme 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NAD-ME1 PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 4.2e-08
Identity = 33/40 (82.50%), Postives = 33/40 (82.50%), Query Frame = 0 

Query: 47  GTAGVAIARALGAVRTQGRLMIDFPKQKIVVAGAG-AGAG 86
           GTAGVAIA  LGAVR QGR MIDFPK KIVVAGAG AG G
Sbjct: 312 GTAGVAIAGLLGAVRAQGRPMIDFPKMKIVVAGAGSAGIG 351

[Close]
BLAST of Sgr027986 vs. ExPASy Swiss-Prot
Match: P37221 (NAD-dependent malic enzyme 62 kDa isoform, mitochondrial OS=Solanum tuberosum OX=4113 PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 4.2e-08
Identity = 33/40 (82.50%), Postives = 33/40 (82.50%), Query Frame = 0 

Query: 47  GTAGVAIARALGAVRTQGRLMIDFPKQKIVVAGAG-AGAG 86
           GTAGVAIA  LGAVR QGR MIDFPK KIVVAGAG AG G
Sbjct: 315 GTAGVAIAGLLGAVRAQGRPMIDFPKMKIVVAGAGSAGIG 354

[Close]
BLAST of Sgr027986 vs. ExPASy Swiss-Prot
Match: P37225 (NAD-dependent malic enzyme 59 kDa isoform, mitochondrial OS=Solanum tuberosum OX=4113 PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 4.3e-05
Identity = 27/40 (67.50%), Postives = 29/40 (72.50%), Query Frame = 0 

Query: 47  GTAGVAIARALGAVRTQGRLMIDFPKQKIVVAGAG-AGAG 86
           GTAGVA+A  LG VR QGR + DF  QKIVV GAG AG G
Sbjct: 298 GTAGVALAGLLGTVRAQGRPLTDFANQKIVVVGAGSAGLG 337

[Close]
BLAST of Sgr027986 vs. ExPASy Swiss-Prot
Match: Q8L7K9 (NAD-dependent malic enzyme 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NAD-ME2 PE=1 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 9.7e-05
Identity = 27/40 (67.50%), Postives = 29/40 (72.50%), Query Frame = 0 

Query: 47  GTAGVAIARALGAVRTQGRLMIDFPKQKIVVAGAG-AGAG 86
           GTAGVA+A  LG VR QGR + DF  QKIVV GAG AG G
Sbjct: 305 GTAGVALAGLLGTVRAQGRPISDFVNQKIVVVGAGSAGLG 344

[Close]
BLAST of Sgr027986 vs. ExPASy TrEMBL
Match: A0A7J6EEH9 (Malic enzyme OS=Cannabis sativa OX=3483 GN=F8388_010958 PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 2.4e-06
Identity = 34/40 (85.00%), Postives = 34/40 (85.00%), Query Frame = 0 

Query: 47  GTAGVAIARALGAVRTQGRLMIDFPKQKIVVAGAG-AGAG 86
           GTAGVAIA  LGAVR QGR MIDFPKQKIVVAGAG AG G
Sbjct: 327 GTAGVAIAGLLGAVRAQGRAMIDFPKQKIVVAGAGSAGIG 366

[Close]
BLAST of Sgr027986 vs. ExPASy TrEMBL
Match: A0A803P6R0 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 2.4e-06
Identity = 34/40 (85.00%), Postives = 34/40 (85.00%), Query Frame = 0 

Query: 47  GTAGVAIARALGAVRTQGRLMIDFPKQKIVVAGAG-AGAG 86
           GTAGVAIA  LGAVR QGR MIDFPKQKIVVAGAG AG G
Sbjct: 327 GTAGVAIAGLLGAVRAQGRTMIDFPKQKIVVAGAGSAGIG 366

[Close]
BLAST of Sgr027986 vs. ExPASy TrEMBL
Match: A0A7J6EYR8 (Malic enzyme OS=Cannabis sativa OX=3483 GN=F8388_022466 PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 2.4e-06
Identity = 34/40 (85.00%), Postives = 34/40 (85.00%), Query Frame = 0 

Query: 47  GTAGVAIARALGAVRTQGRLMIDFPKQKIVVAGAG-AGAG 86
           GTAGVAIA  LGAVR QGR MIDFPKQKIVVAGAG AG G
Sbjct: 327 GTAGVAIAGLLGAVRAQGRTMIDFPKQKIVVAGAGSAGIG 366

[Close]
BLAST of Sgr027986 vs. ExPASy TrEMBL
Match: A0A803P6T5 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 2.4e-06
Identity = 34/40 (85.00%), Postives = 34/40 (85.00%), Query Frame = 0 

Query: 47  GTAGVAIARALGAVRTQGRLMIDFPKQKIVVAGAG-AGAG 86
           GTAGVAIA  LGAVR QGR MIDFPKQKIVVAGAG AG G
Sbjct: 327 GTAGVAIAGLLGAVRAQGRAMIDFPKQKIVVAGAGSAGIG 366

[Close]
BLAST of Sgr027986 vs. ExPASy TrEMBL
Match: A0A7J7NSH4 (Uncharacterized protein OS=Kingdonia uniflora OX=39325 GN=GIB67_038688 PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 2.4e-06
Identity = 41/75 (54.67%), Postives = 50/75 (66.67%), Query Frame = 0 

Query: 15  LVLAQAECDG---VTSIGKITQWQFSSKSYVLSRNGTAGVAIARALGAVRTQGRLMIDFP 74
           L L +   DG   +T I +  +  F+   +V+ + GTAGVAIA  LGAVR QG+ MIDFP
Sbjct: 255 LGLQEHRLDGEEYITVIDEFMEAVFTRWPHVIVQ-GTAGVAIAGLLGAVRAQGKSMIDFP 314

Query: 75  KQKIVVAGAG-AGAG 86
           KQKIVVAGAG AG G
Sbjct: 315 KQKIVVAGAGSAGIG 328

[Close]
BLAST of Sgr027986 vs. TAIR 10
Match: AT2G13560.1 (NAD-dependent malic enzyme 1 )

HSP 1 Score: 58.5 bits (140), Expect = 3.0e-09
Identity = 33/40 (82.50%), Postives = 33/40 (82.50%), Query Frame = 0 

Query: 47  GTAGVAIARALGAVRTQGRLMIDFPKQKIVVAGAG-AGAG 86
           GTAGVAIA  LGAVR QGR MIDFPK KIVVAGAG AG G
Sbjct: 312 GTAGVAIAGLLGAVRAQGRPMIDFPKMKIVVAGAGSAGIG 351

[Close]
BLAST of Sgr027986 vs. TAIR 10
Match: AT4G00570.1 (NAD-dependent malic enzyme 2 )

HSP 1 Score: 47.4 bits (111), Expect = 6.9e-06
Identity = 27/40 (67.50%), Postives = 29/40 (72.50%), Query Frame = 0 

Query: 47  GTAGVAIARALGAVRTQGRLMIDFPKQKIVVAGAG-AGAG 86
           GTAGVA+A  LG VR QGR + DF  QKIVV GAG AG G
Sbjct: 305 GTAGVALAGLLGTVRAQGRPISDFVNQKIVVVGAGSAGLG 344

The following BLAST results are available for this feature:
BLAST of Sgr027986 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_021615290.19.9e-0776.00NAD-dependent malic enzyme 62 kDa isoform, mitochondrial-like isoform X3 [Maniho... [more]
XP_030494112.14.9e-0685.00NAD-dependent malic enzyme 62 kDa isoform, mitochondrial-like [Cannabis sativa] ... [more]
KAF6170155.14.9e-0654.67hypothetical protein GIB67_038688 [Kingdonia uniflora][more]
XP_030493995.14.9e-0685.00NAD-dependent malic enzyme 62 kDa isoform, mitochondrial [Cannabis sativa] >KAF4... [more]
OEL34676.16.4e-0645.83NAD-dependent malic enzyme 1, mitochondrial [Dichanthelium oligosanthes][more]
BLAST of Sgr027986 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P372241.1e-0857.69NAD-dependent malic enzyme 65 kDa isoform, mitochondrial OS=Amaranthus hypochond... [more]
Q9SIU04.2e-0882.50NAD-dependent malic enzyme 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=N... [more]
P372214.2e-0882.50NAD-dependent malic enzyme 62 kDa isoform, mitochondrial OS=Solanum tuberosum OX... [more]
P372254.3e-0567.50NAD-dependent malic enzyme 59 kDa isoform, mitochondrial OS=Solanum tuberosum OX... [more]
Q8L7K99.7e-0567.50NAD-dependent malic enzyme 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=N... [more]
BLAST of Sgr027986 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A7J6EEH92.4e-0685.00Malic enzyme OS=Cannabis sativa OX=3483 GN=F8388_010958 PE=3 SV=1[more]
A0A803P6R02.4e-0685.00Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A7J6EYR82.4e-0685.00Malic enzyme OS=Cannabis sativa OX=3483 GN=F8388_022466 PE=3 SV=1[more]
A0A803P6T52.4e-0685.00Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A7J7NSH42.4e-0654.67Uncharacterized protein OS=Kingdonia uniflora OX=39325 GN=GIB67_038688 PE=3 SV=1[more]
BLAST of Sgr027986 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G13560.13.0e-0982.50NAD-dependent malic enzyme 1 [more]
AT4G00570.16.9e-0667.50NAD-dependent malic enzyme 2 [more]
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr027986.1Sgr027986.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006108 malate metabolic process
biological_process GO:0006090 pyruvate metabolic process
molecular_function GO:0004471 malate dehydrogenase (decarboxylating) (NAD+) activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0051287 NAD binding