Sgr027850 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr027850
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptionthiamine-repressible mitochondrial transport protein THI74-like isoform X3
Locationtig00153055: 3229944 .. 3234208 (+)
RNA-Seq ExpressionSgr027850
SyntenySgr027850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTGGAGTTATAGGATTGGTTTGGGGTTGATTTGTACGGCGGTTGTGATATGGGTTACTTCGGCGGAGATTACCCAGGTTTGGTCTGATTCCGGTGGAATTGTCTTCGTTTAACTGAGTGTTTTTTTTTTTTTTTTGCTTTGCATTTTGGGATTAATCGCTGTTTTTTGTTTGTTGACTTTGTTTATTTGCTGATGTTATTTGTTGGGCAAAGAAATGAAAACCAGAGCTTCGATTGTTTTGTTTTATTCAGTTAATTACAACATTAATTACGTCGAATCATAGAATCGCTCCTACATGCATGGACTGATCAGTGCCTGGAGCGCATGTCTGTGACATGTTCGGGGAAATAACGACCCACCTTGATGATTTGGACGTGGATGCCATCTTTGATTATTCTTTTTGCTTGGCTTTATGTTCCCCACTGTTCTTTTACTTTTGATTTCACACTAATTAAAATTTGTGTTATTTGGGGGACGTCGCTAATATTTTCCTTAACTAGTTAAGTCATACTTAGTTTGGCACTTTTCACGTGTTAATATTTAGTTCCAATTAAGAATATATATATTAGGACGTTTAGGTCGTGTCTAATAAATTCTTGTATCTTAAAATTTTTTTAATAGGTTTCTAAACTTTCAAAGTTGCATTTATTAGGTTCTTATACTTTAAAGGTTTTTAATAGGTCCCTAAACTTTTAATTTTGTATCAAATAGGTATATGTCTTTAACTCTTTTGGGCAAATATTTGAATTTGTCCGAAATTAGTTTGTTACATAATCCTTACATAATACAATCAACATGTAATGTAAGCGTTAAACCATCGGAGTTAATGCAAGGATCTATTAGACACAAAATTGAAAGTTTAAGAACCTATTAGAAATCATAAATACAGAGAACTATTAATCACAACTTTGAAAGTCTAGGAACTTATTAGAAACTTTTCAAAGTACATGGATCTATTAGGACACAATCCAGAAAGTCAGGGAATGTTATTTAACCAAAATATTAAATATACTGAACTTGTATAAGTTTTTTGATTTAGCAGTGATTGAGGTCCTAGGTTTGCATTGTTATTCTTGTTGGGAAAATTACAACTTACCCCATACTTGTGTGGTGCAGAGAATCTTCAGTGAATATAAACATCCTTTTGCACTCACTTACTTGGGGGTGTCTCTTATGGTGGTATATCTACCAGTGGCAGTTGTCAAAGACTTTGTCTGCAGTTTACTAAACAACCATCCTGATAATGATGGCAGTGTTCTGAGCTCTTCCATTGGACCTGACATCCCTTTGAGATTTAATGAAGTACAATACAGTTTGGATGAAGACATGGAAAGCTGCCTAATTAGTGACAAAGATTTGAGTGAAAGGGAAGAGGTCAGCCTCTGATTCCTAAGAATGAATCACACCATGAAGTTAGTACATGGGAAATTGCTAAATGCAGCCTTTACCTTACTCCTTTGTGGTTCACTACAGAGGTACAGAAAGTTTGAACTATTTTAGTTGGTTGACTTTGCAGATTTTTTTGTTTTACATTGTCTACATGTTCTTTTAGCTGCTTCAATGCTTATATTATCAATCATCAGAAAGATAACTCAATGACACTGTTATTAGATGGGCTTCTTTTGATATAGTAGCTATTAGTCTGCTTAATTATGAGAATTGTTTAGGATGTCTGAACAAATGTGTTTTTAGCTTGACATTCATTGACTTGATAGTGTTTTTGTTTTTGGTTTTTGGTTTTTAAAATTTATGCTTATTTACTAATAATTTCATTTGTGTTCTAATCTACTTTCAAAAACATTTTTGAAATCTTAGCCAAAATTTGAAAAGTAAAAACAAACCTGTTTTTCGTTTTAAAACTGTTTTTGCAATTTGGCTGAGATCATCATTTATCATTGATTGTTAATTCTAGCATTTCATTTAGCTGCGTTGTCATTTTGATAAATTTGTTCGAGACTTTTGCAGTATTTCTCAAACTCGGCACTGGCAAATACCAGCGTTGCAAGCGCAACCATCTTAAATTCTACGTCGGGCCTGTTTGCCCTTTTGTTTGGAGCTCTTCTTGGACAAGAATCCATAACCATTTCCAAGGTGGTAGCTGTTTTTATTAGCATGGCTGGGGTTGCCATGACAACCCTGGGAAAAACTTGGGCAACTAATGAGTTCCTAATCATCTCTGAGTAAGTGATGCTGTTGATTAGCATAGTATTTATGATCTCTATTTCAAAGTTCAGATAAAATCATCACATGTTACATTTCTTTTGGCCAAGAGCTTAACCTTCTCTTTCTCTCTTTTTATTAATCTGTGGTATTGGGCCCCACCCGACTACCCACGGGGAGACTTCACATGCCCCAATGCCTCATCGATGGGATTTTCGGACCTTGAGGAACTACCCCTAAATATTTAAGCACTTGCCAACCCTTTGAGCTTATATATTTTACATTTTACATTGGTTTAGATGATCAGTTGGAGGTGGCAATTCCAACTTAAAATGAAGAGAAGTTCATGTCTTTCTATAACTTATTTGGACTATATGGTTACAAAGTTTTGATTGTTTCTGTAATGTATTATATTTCTTCCTTCCTTGGTAAGCTCTAGAGAGAGAACCGTCACGGGGGACATTTTCGGTCTTCTTTCTGCAGTAATCTATGGATTATTTACTGGTATGCCACTCTAACTGGGAACTGTTTTGTTGATAATTTAAATGTTTTACTTCATGATAATTATTTACTTCTTGTGTGATTGGTAATTGGTATAGTGCTTCTCAAGAAATCAGCTGGATCAGAAGGAGACAAGATTGATGTGCAAAAGTTCTTTGGATTTGTTGGTCTCTTTACTGTACTGGGTCTTTGGTGGCTTGGTAAGTGGATCATTCACAATGCTAATGATGCTTTGTTCTTCTTGATTCCAAAATTTTTTAGGGTTGGATAGAATAATTTGGCAATGATTGGAAAATTAAGGACTTCATTGACTTGAGTTTAGACTGCTTATTAAATTTAAGGCAATTTGGTCGTTTGGCTCTGAAATTTTTTTGTTACATTCTGCCTTTTCAATAAATGGTCATCAATGTTTTTGCTACCTTGACTTGCACTGTTCTTACTATTAAAGTCACTCTGCAGTGTGGCCTTTAACTGCTGTGGGCATTGAACCTCCTCTCAAGTTTCCTCCCTCGAAATCCGTCACTGAAGTTGTGCTTTTAAATGGCTTCGTCGGGAGCGTTCTGTCAGATTACTTCTGGTACGTCGTGAATACTATATATGTTTGGGACATCGAGAATAATGTACATGTTTGAAATGACCACCGATCCCATAGGATAACGATTTTGTTTTTGGTTTTGAGTTTTTTTAAAAAATTAAGCTCAGGGAAGTTTAGTTTTTTATCATTTTGAAATATTTTAATCATTTTTTTAAAGAAGCAATGATTTTTAGAAAACGAAAAATTAGGAACGAAAACGTTATCAAACAGAAGACCTTAGCAGCTCAACAGCTTAATATGTTTTTTTGTTTCTTGGCATATTAAGCCATTGATATCACTACATTTCTACATTTAAGTAGTTGCCTTTTTGACAACTTTGCTGAAACTTTGCTCTCTTTCATCCAAAAAATTCCTTATTGCATGGAGAATTCTATCATACTTGTAGGGCCCTCTCTGTGATTTGGACATCTCCATTAGTTGCTACTCTGGGCATGTCATTGACAATTCCCCTTGCTATGCTGGCAGACGTGATATTGCACGGCCGTCGCTACTCCGCCCTCTACATCCTCGGTTGCGTTCAGGTAAACGCTCATCGACTCGCCCCGCTTTGTCTATCTTTGCTTGATAGTCCCTATAATTTTTTTTTCATTATGTTTATTTTTAGTCCATGTTGTTAACCGTAACACGAATTAATAGGAATCAAATGATATTCATGTCGTGTGGTGTGATTTGGAGGCCAGTATGGATTTGCAAGATTAATTAGGCAAATTCAGTAACTAAACATATAATTTGATCTTGAAAAAAATATTCACAAAATTATCTATTCATTTATTTTTAATCCGTTCAAATGACTGTTAGATCATGTCACTCCATCGGAGACTTGTTAGCCCTATTTTTCTGCTCTCCATTCAGGTATTTGCTGGATTTTTCATAGCAAATTTTTCAGACAAGTTCTCTGCCTAGGGCAACCAGTTACAGTGACTGCTGAATCCTCTGAAGAAGAGGAGGGTGTACAGAGATTTGGTGCACTTTGGAAGTATGAGCTGTATTGTCATGTAATGTAA

mRNA sequence

ATGGGTTGGAGTTATAGGATTGGTTTGGGGTTGATTTGTACGGCGGTTGTGATATGGGTTACTTCGGCGGAGATTACCCAGAGAATCTTCAGTGAATATAAACATCCTTTTGCACTCACTTACTTGGGGGTGTCTCTTATGGTGGTATATCTACCAGTGGCAGTTGTCAAAGACTTTGTCTGCAGTTTACTAAACAACCATCCTGATAATGATGGCAGTGTTCTGAGCTCTTCCATTGGACCTGACATCCCTTTGAGATTTAATGAAGTACAATACAGTTTGGATGAAGACATGGAAAGCTGCCTAATTAGTGACAAAGATTTGAGTGAAAGGGAAGAGTATTTCTCAAACTCGGCACTGGCAAATACCAGCGTTGCAAGCGCAACCATCTTAAATTCTACGTCGGGCCTGTTTGCCCTTTTGTTTGGAGCTCTTCTTGGACAAGAATCCATAACCATTTCCAAGGTGGTAGCTGTTTTTATTAGCATGGCTGGGGTTGCCATGACAACCCTGGGAAAAACTTGGGCAACTAATGAGTTCCTAATCATCTCTGACTCTAGAGAGAGAACCGTCACGGGGGACATTTTCGGTCTTCTTTCTGCAGTAATCTATGGATTATTTACTGTGCTTCTCAAGAAATCAGCTGGATCAGAAGGAGACAAGATTGATGTGCAAAAGTTCTTTGGATTTGTTGGTCTCTTTACTGTACTGGGTCTTTGGTGGCTTGTGTGGCCTTTAACTGCTGTGGGCATTGAACCTCCTCTCAAGTTTCCTCCCTCGAAATCCGTCACTGAAGTTGTGCTTTTAAATGGCTTCGTCGGGAGCGTTCTGTCAGATTACTTCTGGGCCCTCTCTGTGATTTGGACATCTCCATTAGTTGCTACTCTGGGCATGTCATTGACAATTCCCCTTGCTATGCTGGCAGACGTGATATTGCACGGCCGTCGCTACTCCGCCCTCTACATCCTCGGTTGCGTTCAGGAATCAAATGATATTCATGTCGTGTGGTGTGATTTGGAGGCCAGTATTTGCTGGATTTTTCATAGCAAATTTTTCAGACAAGTTCTCTGCCTAGGGCAACCAGTTACAGTGACTGCTGAATCCTCTGAAGAAGAGGAGGGTGTACAGAGATTTGGTGCACTTTGGAAGTATGAGCTGTATTGTCATGTAATGTAA

Coding sequence (CDS)

ATGGGTTGGAGTTATAGGATTGGTTTGGGGTTGATTTGTACGGCGGTTGTGATATGGGTTACTTCGGCGGAGATTACCCAGAGAATCTTCAGTGAATATAAACATCCTTTTGCACTCACTTACTTGGGGGTGTCTCTTATGGTGGTATATCTACCAGTGGCAGTTGTCAAAGACTTTGTCTGCAGTTTACTAAACAACCATCCTGATAATGATGGCAGTGTTCTGAGCTCTTCCATTGGACCTGACATCCCTTTGAGATTTAATGAAGTACAATACAGTTTGGATGAAGACATGGAAAGCTGCCTAATTAGTGACAAAGATTTGAGTGAAAGGGAAGAGTATTTCTCAAACTCGGCACTGGCAAATACCAGCGTTGCAAGCGCAACCATCTTAAATTCTACGTCGGGCCTGTTTGCCCTTTTGTTTGGAGCTCTTCTTGGACAAGAATCCATAACCATTTCCAAGGTGGTAGCTGTTTTTATTAGCATGGCTGGGGTTGCCATGACAACCCTGGGAAAAACTTGGGCAACTAATGAGTTCCTAATCATCTCTGACTCTAGAGAGAGAACCGTCACGGGGGACATTTTCGGTCTTCTTTCTGCAGTAATCTATGGATTATTTACTGTGCTTCTCAAGAAATCAGCTGGATCAGAAGGAGACAAGATTGATGTGCAAAAGTTCTTTGGATTTGTTGGTCTCTTTACTGTACTGGGTCTTTGGTGGCTTGTGTGGCCTTTAACTGCTGTGGGCATTGAACCTCCTCTCAAGTTTCCTCCCTCGAAATCCGTCACTGAAGTTGTGCTTTTAAATGGCTTCGTCGGGAGCGTTCTGTCAGATTACTTCTGGGCCCTCTCTGTGATTTGGACATCTCCATTAGTTGCTACTCTGGGCATGTCATTGACAATTCCCCTTGCTATGCTGGCAGACGTGATATTGCACGGCCGTCGCTACTCCGCCCTCTACATCCTCGGTTGCGTTCAGGAATCAAATGATATTCATGTCGTGTGGTGTGATTTGGAGGCCAGTATTTGCTGGATTTTTCATAGCAAATTTTTCAGACAAGTTCTCTGCCTAGGGCAACCAGTTACAGTGACTGCTGAATCCTCTGAAGAAGAGGAGGGTGTACAGAGATTTGGTGCACTTTGGAAGTATGAGCTGTATTGTCATGTAATGTAA

Protein sequence

MGWSYRIGLGLICTAVVIWVTSAEITQRIFSEYKHPFALTYLGVSLMVVYLPVAVVKDFVCSLLNNHPDNDGSVLSSSIGPDIPLRFNEVQYSLDEDMESCLISDKDLSEREEYFSNSALANTSVASATILNSTSGLFALLFGALLGQESITISKVVAVFISMAGVAMTTLGKTWATNEFLIISDSRERTVTGDIFGLLSAVIYGLFTVLLKKSAGSEGDKIDVQKFFGFVGLFTVLGLWWLVWPLTAVGIEPPLKFPPSKSVTEVVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSLTIPLAMLADVILHGRRYSALYILGCVQESNDIHVVWCDLEASICWIFHSKFFRQVLCLGQPVTVTAESSEEEEGVQRFGALWKYELYCHVM
Homology
BLAST of Sgr027850 vs. NCBI nr
Match: XP_022142439.1 (uncharacterized vacuolar membrane protein YML018C-like [Momordica charantia])

HSP 1 Score: 546.2 bits (1406), Expect = 2.3e-151
Identity = 294/366 (80.33%), Postives = 308/366 (84.15%), Query Frame = 0

Query: 1   MGWSYRIGLGLICTAVVIWVTSAEITQRIFSEYKHPFALTYLGVSLMVVYLPVAVVKDFV 60
           MGW YRIGLGLI TAVVIWVTSAEITQRIFSEYKHPFAL+YLGVSLMVVYLPVAVV+D +
Sbjct: 1   MGWRYRIGLGLILTAVVIWVTSAEITQRIFSEYKHPFALSYLGVSLMVVYLPVAVVRDLI 60

Query: 61  CSLL-----NNHPDNDGSVLSSSIGPDIPLRFNEVQYSLDEDMESCLISDKDLSERE--- 120
            SLL     NNHPDN GSVLSSSIG D PLRFNE   SLDE M SCLISDKDLSERE   
Sbjct: 61  FSLLNPDLPNNHPDNGGSVLSSSIGLDFPLRFNEAHNSLDEGMGSCLISDKDLSEREEGQ 120

Query: 121 -------------------------------EYFSNSALANTSVASATILNSTSGLFALL 180
                                          EYFSNSALANTSVA+ATILNSTSGLFALL
Sbjct: 121 PLIPKIEPKNEVSSWEIAKCSLFLTPLWFTTEYFSNSALANTSVATATILNSTSGLFALL 180

Query: 181 FGALLGQESITISKVVAVFISMAGVAMTTLGKTWATNEFLIISDSRERTVTGDIFGLLSA 240
           FGA+LGQESITISKVVAV ISMAGVAMTTLGKTWATNE+LIIS+SR RTV GD+FGLLSA
Sbjct: 181 FGAVLGQESITISKVVAVLISMAGVAMTTLGKTWATNEYLIISESRGRTVMGDMFGLLSA 240

Query: 241 VIYGLFTVLLKKSAGSEGDKIDVQKFFGFVGLFTVLGLWWLVWPLTAVGIEPPLKFPPSK 300
           V YGLFTVLLKKSAGSEGDKIDVQKFFG+VGLFT+LGLWWLVWPLTAVGIEPPLKFP SK
Sbjct: 241 VTYGLFTVLLKKSAGSEGDKIDVQKFFGYVGLFTILGLWWLVWPLTAVGIEPPLKFPASK 300

Query: 301 SVTEVVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSLTIPLAMLADVILHGRRYSALY 328
           SVTE+VLLNGFVGSV+SDY WA SVIWTSPLVATLGMSLTIPLAMLADV+LHGRRYSALY
Sbjct: 301 SVTEIVLLNGFVGSVVSDYLWAHSVIWTSPLVATLGMSLTIPLAMLADVVLHGRRYSALY 360

BLAST of Sgr027850 vs. NCBI nr
Match: XP_038899552.1 (uncharacterized vacuolar membrane protein YML018C-like [Benincasa hispida])

HSP 1 Score: 533.5 bits (1373), Expect = 1.5e-147
Identity = 281/364 (77.20%), Postives = 308/364 (84.62%), Query Frame = 0

Query: 1   MGWSYRIGLGLICTAVVIWVTSAEITQRIFSEYKHPFALTYLGVSLMVVYLPVAVVKDFV 60
           MG  Y+ GLGLICTAV+IWV SAEITQRIFSEYKHPFA++YLGVSLMV+YLP+AVVKD V
Sbjct: 55  MGCKYKFGLGLICTAVLIWVASAEITQRIFSEYKHPFAISYLGVSLMVIYLPMAVVKDLV 114

Query: 61  CSLLNNHP--DNDGSVLSSSIGPDIPLRFNEVQYSLDEDM-ESCLISDKDLSERE----- 120
           CSL+N H   DN+GSV+SSSIG D PLRFN+V Y+ D+ M  S +I DKDLSERE     
Sbjct: 115 CSLINPHNLLDNNGSVMSSSIGLDGPLRFNDVHYNADDSMGHSLIIRDKDLSEREEGHPL 174

Query: 121 -----------------------------EYFSNSALANTSVASATILNSTSGLFALLFG 180
                                        EYFSNSALANTSVA+ATILNSTSGLFALLFG
Sbjct: 175 IPNFESHHKVSTWEVIKCSIYLTPLWFSTEYFSNSALANTSVATATILNSTSGLFALLFG 234

Query: 181 ALLGQESITISKVVAVFISMAGVAMTTLGKTWATNEFLIISDSRERTVTGDIFGLLSAVI 240
           AL GQESITISKVVAVFISMAGV MTTLGKTWATNEFLIIS+SR +T+TGD+FGLLSA I
Sbjct: 235 ALFGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISESRGQTITGDVFGLLSAAI 294

Query: 241 YGLFTVLLKKSAGSEGDKIDVQKFFGFVGLFTVLGLWWLVWPLTAVGIEPPLKFPPSKSV 300
           YGLFTVLLKKSAGSEGDKIDVQKFFG+VGLFT+LGLWWLVWPLTAVGIEPPLKFPPSKS+
Sbjct: 295 YGLFTVLLKKSAGSEGDKIDVQKFFGYVGLFTILGLWWLVWPLTAVGIEPPLKFPPSKSI 354

Query: 301 TEVVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSLTIPLAMLADVILHGRRYSALYIL 328
           +E++LLNGF+GSVLSDYFWALSVIWTSPLVATLGMSLTIPLAMLADV+LHGRRYSALYIL
Sbjct: 355 SEILLLNGFIGSVLSDYFWALSVIWTSPLVATLGMSLTIPLAMLADVVLHGRRYSALYIL 414

BLAST of Sgr027850 vs. NCBI nr
Match: XP_008452823.1 (PREDICTED: uncharacterized transporter C405.03c-like [Cucumis melo])

HSP 1 Score: 531.2 bits (1367), Expect = 7.7e-147
Identity = 286/368 (77.72%), Postives = 309/368 (83.97%), Query Frame = 0

Query: 1   MGWSYRIGLGLICTAVVIWVTSAEITQRIFSEYKHPFALTYLGVSLMVVYLPVAVVKDFV 60
           MG  Y+IGLGLICTAV+IWV SAEITQRIFSEYKHPFAL+YLGVSLMV+YLPVAV+KD V
Sbjct: 1   MGCKYKIGLGLICTAVLIWVASAEITQRIFSEYKHPFALSYLGVSLMVIYLPVAVLKDLV 60

Query: 61  CSLLNNH---PDNDGSVLSSSIGPDIPLRFNEVQYSLDEDMESCL-ISDKDLS-ERE--- 120
           CSLLN H    +N GS++S+SIG D PLRFNE+ Y+LD  M  CL I+DKDLS ERE   
Sbjct: 61  CSLLNPHNLLDNNYGSIISTSIGIDGPLRFNEMHYNLDGSMGHCLIINDKDLSTEREELG 120

Query: 121 ---------------------------------EYFSNSALANTSVASATILNSTSGLFA 180
                                            EYFSNSALANTSVA+ATILNSTSGLFA
Sbjct: 121 QPLIPNFESSHHKVSTWEIIKCSLYLTPLWFTTEYFSNSALANTSVATATILNSTSGLFA 180

Query: 181 LLFGALLGQESITISKVVAVFISMAGVAMTTLGKTWATNEFLIISDSRERTVTGDIFGLL 240
           LLFGAL+GQESITISKVVAVFISMAGV MTTLGKTWATNEFLIIS+ R R++TGDIFGLL
Sbjct: 181 LLFGALVGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISEPRGRSITGDIFGLL 240

Query: 241 SAVIYGLFTVLLKKSAGSEGDKIDVQKFFGFVGLFTVLGLWWLVWPLTAVGIEPPLKFPP 300
           SA IYGLFTVLLKKSAGSEGDKIDVQKFFG+VGLFT+LGLWWLVWPLTAVGIEPPLKFPP
Sbjct: 241 SAAIYGLFTVLLKKSAGSEGDKIDVQKFFGYVGLFTILGLWWLVWPLTAVGIEPPLKFPP 300

Query: 301 SKSVTEVVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSLTIPLAMLADVILHGRRYSA 328
           SKS+TE+VLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSLTIPLAMLADV+LHGRRYSA
Sbjct: 301 SKSITEIVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSLTIPLAMLADVVLHGRRYSA 360

BLAST of Sgr027850 vs. NCBI nr
Match: XP_004145580.1 (uncharacterized transporter C405.03c [Cucumis sativus])

HSP 1 Score: 527.7 bits (1358), Expect = 8.5e-146
Identity = 286/368 (77.72%), Postives = 308/368 (83.70%), Query Frame = 0

Query: 1   MGWSYRIGLGLICTAVVIWVTSAEITQRIFSEYKHPFALTYLGVSLMVVYLPVAVVKDFV 60
           MG  Y+IGLGLICTAV+IWV SAEITQRIFSEYKHPFAL+YLGVSLMV+YLPVAV+KD V
Sbjct: 1   MGCKYKIGLGLICTAVLIWVASAEITQRIFSEYKHPFALSYLGVSLMVIYLPVAVLKDLV 60

Query: 61  CSLLNNH---PDNDGSVLSSSIGPDIPLRFNEVQYSLDEDMESCL-ISDKDLS--ERE-- 120
           CSLLN H    +N  S++S+SIG D PLRFNE+ Y+LDE M  CL I+DKDLS  ERE  
Sbjct: 61  CSLLNPHSLLDNNYDSIISTSIGIDGPLRFNEIHYNLDESMGHCLIINDKDLSTTEREEG 120

Query: 121 ---------------------------------EYFSNSALANTSVASATILNSTSGLFA 180
                                            EYFSNSALANTSVA+ATILNSTSGLFA
Sbjct: 121 QPLIPNFESSHHKVSTWEIIKCSLYLTPLWFTTEYFSNSALANTSVATATILNSTSGLFA 180

Query: 181 LLFGALLGQESITISKVVAVFISMAGVAMTTLGKTWATNEFLIISDSRERTVTGDIFGLL 240
           LLFGAL+GQESITISKVVAVFISMAGV MTTLGKTWATNEFLIIS+SR  ++TGDIFGLL
Sbjct: 181 LLFGALVGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISESRGLSITGDIFGLL 240

Query: 241 SAVIYGLFTVLLKKSAGSEGDKIDVQKFFGFVGLFTVLGLWWLVWPLTAVGIEPPLKFPP 300
           SA IYGLFTVLLKKSAGSEGDKIDVQKFFG+VGLFTVLGLWWLVWPLTA+GIEPPLKFPP
Sbjct: 241 SAAIYGLFTVLLKKSAGSEGDKIDVQKFFGYVGLFTVLGLWWLVWPLTAMGIEPPLKFPP 300

Query: 301 SKSVTEVVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSLTIPLAMLADVILHGRRYSA 328
           S S+TE+VLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSLTIPLAMLADVILHGRRYSA
Sbjct: 301 STSLTEIVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSLTIPLAMLADVILHGRRYSA 360

BLAST of Sgr027850 vs. NCBI nr
Match: XP_023524239.1 (thiamine-repressible mitochondrial transport protein THI74-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 521.2 bits (1341), Expect = 7.9e-144
Identity = 274/366 (74.86%), Postives = 302/366 (82.51%), Query Frame = 0

Query: 1   MGWSYRIGLGLICTAVVIWVTSAEITQRIFSEYKHPFALTYLGVSLMVVYLPVAVVKDFV 60
           MG  Y+ GLGLIC AV+IWV SAEITQRIF+EYK PFAL+YLGVSLMVVYLPVAVVKD V
Sbjct: 1   MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV 60

Query: 61  CS-----LLNNHPDNDGSVLSSSIGPDIPLRFNEVQYSLDEDMESCLISDKDLSERE--- 120
           CS     LLN+H +NDGSV SSSIG D+PL+FNE+ Y  DED++SCL SDKDLSERE   
Sbjct: 61  CSLLNPDLLNDHHENDGSVPSSSIGLDVPLKFNEIHYKFDEDLKSCLTSDKDLSEREEGQ 120

Query: 121 -------------------------------EYFSNSALANTSVASATILNSTSGLFALL 180
                                          EYFSNSALANTSVA+ATILNSTSGLFALL
Sbjct: 121 PLMSNFESHHKVSTREIIERSLYLTPIWFTTEYFSNSALANTSVATATILNSTSGLFALL 180

Query: 181 FGALLGQESITISKVVAVFISMAGVAMTTLGKTWATNEFLIISDSRERTVTGDIFGLLSA 240
           FGAL+GQESITI+KVVAVFISMAGV MTTLGKTWATN F +IS+SR RT+TGD+F LLSA
Sbjct: 181 FGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESRGRTITGDVFCLLSA 240

Query: 241 VIYGLFTVLLKKSAGSEGDKIDVQKFFGFVGLFTVLGLWWLVWPLTAVGIEPPLKFPPSK 300
            IYGLFTVLLKK AGS GDKIDVQKFFG+VGLFT++GLWWLVWPLTA+GIEPP KFPPSK
Sbjct: 241 AIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSK 300

Query: 301 SVTEVVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSLTIPLAMLADVILHGRRYSALY 328
           S+TE+V+LNGFVG+VLSDYFWALSVIWTSPLV+ LGMSLTIPLAMLADVILHGRRYS LY
Sbjct: 301 SITEIVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADVILHGRRYSTLY 360

BLAST of Sgr027850 vs. ExPASy Swiss-Prot
Match: Q03730 (Uncharacterized vacuolar membrane protein YML018C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YML018C PE=1 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 5.3e-34
Identity = 113/345 (32.75%), Postives = 178/345 (51.59%), Query Frame = 0

Query: 5   YRIGLGLICTAVVIWVTSAEITQRIFSE--YKHPFALTYLGVSLMVVYL---PVAVVKDF 64
           + +GL ++   +++WV S+ +   IF +  Y+ PF +TY   +  + YL     AVV ++
Sbjct: 13  WTLGLLMLGLVIILWVLSSFLINLIFEDDSYRKPFFITYTNTAAFIFYLFPTAKAVVVNY 72

Query: 65  -------VCSLLNNHPDNDGSVLSSSIGPDIPLRFN-EVQYSLDEDMESCLISDKDLSER 124
                  V   L    +  GS  + S+    PL  N E     ++     L     LS  
Sbjct: 73  KDTGRANVHRELIMEEEGTGSDSNRSVDMTSPLLTNLEAGTHANQKKRLTLYETIKLSAE 132

Query: 125 -------EEYFSNSALANTSVASATILNSTSGLFALLFGALLGQESITISKVVAVFISMA 184
                      +N++LA TSVAS TIL++TS  F L  GA+   ES++ SKV+  FIS  
Sbjct: 133 FCILWFTANLVTNASLAFTSVASQTILSTTSSFFTLFIGAICHVESLSKSKVLGSFISFV 192

Query: 185 GVAMTTLGKTWATNEFLIISDSRE-----RTVTGDIFGLLSAVIYGLFTVLLKKSAGSEG 244
           G+ M T   +    +  I   S +     + + G++  L  AV+YG+++ LLK+  G E 
Sbjct: 193 GIIMVTKSDSHQRYQRHIADVSGDDNDAVQVLIGNLLALAGAVLYGVYSTLLKREVGDE- 252

Query: 245 DKIDVQKFFGFVGLFTVLGLWWLVWPLTAVGIEPPLKFPPSKSVTEVVLLNGFVGSVLSD 304
            +++++ FFGFVGLF +L LW  +  L   G E P   P    V  ++ +N  + + +SD
Sbjct: 253 TRVNMKIFFGFVGLFNLLFLWPSLIVLDFFGWE-PFSLPKDPKVVVIIFVNCLI-TFVSD 312

Query: 305 YFWALSVIWTSPLVATLGMSLTIPLAMLADVILHGRRYSALYILG 325
           + WA +++ TSPL  T+G+S+TIPLAM  DVI   +  SALY+ G
Sbjct: 313 FCWAKAMLLTSPLTVTVGLSITIPLAMFGDVIFKHKTMSALYLFG 354

BLAST of Sgr027850 vs. ExPASy Swiss-Prot
Match: O94654 (Uncharacterized transporter C405.03c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC405.03c PE=3 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 8.0e-30
Identity = 99/328 (30.18%), Postives = 169/328 (51.52%), Query Frame = 0

Query: 5   YRIGLGLICTAVVIWVTSAEITQRIFSE--YKHPFALTYLGVSLMVVYL------PVAVV 64
           + +G+ L+   V +W+ S+ +T  +  +  +  PF +TY+     V YL           
Sbjct: 9   HALGVVLLLFVVFLWLISSFLTSSLLDDDNFFSPFLITYINTGTFVFYLIPWYFSEKKTR 68

Query: 65  KDFVCSLLNNHPDNDGSVLSSSIGPDIPLRFNEVQYSLDEDMESCLISDKDLSEREEYFS 124
           K  + S L+ +     S  +    P+ PL F +  Y     +  C+I          YFS
Sbjct: 69  KHRLMSELSMYESVHDSSFNLGTRPNSPLGFRQTAYL---SLGFCII-----WFAANYFS 128

Query: 125 NSALANTSVASATILNSTSGLFALLFGALLGQESITISKVVAVFISMAGVAMTTLGKTWA 184
           NS+L  T+VAS TI++S SG F L  G ++  E  T+SK++A+  S+ GV +     T  
Sbjct: 129 NSSLGFTNVASFTIISSMSGFFTLGLGTIVNVERFTLSKLLALMASVGGVIIVV---TQD 188

Query: 185 TNEFLIISDSRERTVTGDIFGLLSAVIYGLFTVLLKKSAGSEGDKIDVQKFFGFVGLFTV 244
             +  +      R   G+ + LL+A++YG ++V++K    +E   +  + FFG VGLF +
Sbjct: 189 AKQADLNDSPPSRPALGNAYALLAALLYGCYSVMVKFHI-TEESCVSTRLFFGLVGLFDL 248

Query: 245 LGLWWLVWPLTAVGIEPPLKFPPSKSVTEVVLLNGFVGSVLSDYFWALSVIWTSPLVATL 304
           + LW  +  L   G+E     P + +   V+++N  + + +SDY W ++++ TSPL+ T+
Sbjct: 249 ILLWPFLIILHLYGVE-RFSLPSTTAGLIVLIINASI-TFVSDYLWVIAMLMTSPLLVTV 308

Query: 305 GMSLTIPLAMLADVILHGRRYSALYILG 325
           GMSL+IPLA+  D++L G   +   ILG
Sbjct: 309 GMSLSIPLALFFDILLKGHYLNFSLILG 322

BLAST of Sgr027850 vs. ExPASy Swiss-Prot
Match: A6QL92 (Solute carrier family 35 member F5 OS=Bos taurus OX=9913 GN=SLC35F5 PE=2 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 1.5e-28
Identity = 109/402 (27.11%), Postives = 185/402 (46.02%), Query Frame = 0

Query: 7   IGLGLICTAVVIWVTSAEITQRIFSEYKHPFALTYLGVSLMVVYLPVAVV---------K 66
           +G+ ++    VIWV S+E+T  +F++Y  PF  T+   S+ V+YL   +V         +
Sbjct: 71  LGIVILLLVDVIWVASSELTSYVFTQYNKPFFSTFAKTSMFVLYLLGFIVWKPWRQQCTR 130

Query: 67  DFVCSLLNNHPDNDG--------SVLSSSIGPD--IPLRFNE------------------ 126
            F         D +G        + ++SS+     +P++F++                  
Sbjct: 131 GFRGKHATFFADAEGYFAACTTDTTMNSSLSEPLYVPVKFHDLPSEKPENTNIDTEKIPK 190

Query: 127 ---------------------------VQYSLDEDMESCLISDKDLSERE---------- 186
                                      + Y   ++ ES L +   L+  +          
Sbjct: 191 KSRVRFSNIMEIRQLPSSHALEAKLSRMSYPTVKEQESLLKTVGKLTATQVAKISFFFCF 250

Query: 187 -----EYFSNSALANTSVASATILNSTSGLFALLFGALLGQES---ITISKVVAVFISMA 246
                 +    AL++T VA   IL+STSGLF L+  A+    S    T+SK++AV +S+ 
Sbjct: 251 VWFLANFSYQEALSDTQVAIVNILSSTSGLFTLILAAMFPSNSGDRFTLSKLLAVILSIG 310

Query: 247 GVAMTTLGKTWATNEFLIISDSRERTVTGDIFGLLSAVIYGLFTVLLKKSAGSEGDKIDV 306
           GV +  L  +           S  R   G I+ L+ A++Y ++ V++K+    E DK+D+
Sbjct: 311 GVVLVNLSGS---------EKSPGRNTIGSIWSLVGAMLYAVYIVMIKRKVDRE-DKLDI 370

Query: 307 QKFFGFVGLFTVLGLWWLVWPLTAVGIEPPLKFPPSKSVTEVVLLNGFVGSVLSDYFWAL 327
             FFGFVGLF +L LW   + L   G E   +F P+K V   +++NG +G+VLS++ W  
Sbjct: 371 PMFFGFVGLFNLLLLWPGFFLLHYTGFE-DFEF-PNKVVLMCIVINGLIGTVLSEFLWLW 430

BLAST of Sgr027850 vs. ExPASy Swiss-Prot
Match: Q04083 (Thiamine-repressible mitochondrial transport protein THI74 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=THI74 PE=1 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 2.0e-28
Identity = 103/340 (30.29%), Postives = 172/340 (50.59%), Query Frame = 0

Query: 5   YRIGLGLICTAVVIWVTSAEITQRIF--SEYKHPFALTYLGVSLMVVYLPV---AVVKDF 64
           + IG+ L+   VV WV ++ +T  +   + Y  PF LTYL +S   +YL      +++  
Sbjct: 10  HMIGVLLLAVVVVFWVGASCLTNELLETNAYNKPFFLTYLNISSFALYLTPDLWRIIQSR 69

Query: 65  VCSLLNNHPDNDGSVLSSSIGPDIPLRFNEVQYSLDEDMESCLISD---KDLSEREEYF- 124
             SL              S    +PL  +    S   ++ S  I+D   KD       F 
Sbjct: 70  RKSLQERTERTLPIHTQESFSEFLPLLSSTP--STSSNLSS--IADTKVKDTMRLSLLFC 129

Query: 125 ---------SNSALANTSVASATILNSTSGLFALLFGALLGQESITISKVVAVFISMAGV 184
                    +N+AL+ T+VAS+TIL+STS  F L     LG E+ +  K++ +F+S+ G+
Sbjct: 130 VLWFVANLAANAALSYTTVASSTILSSTSSFFTLFLATSLGIETFSTKKLLGLFVSLFGI 189

Query: 185 AMTTLGKTWATNEFLIISDSRERTVTGDIFGLLSAVIYGLFTVLLKKSAGSEGDKIDVQK 244
            +  +  +   +     S S    + G+   LL ++ Y ++T LLK    S+G ++D+Q 
Sbjct: 190 ILIVMQSSKQQD-----SVSASSFLVGNTLALLGSLGYSVYTTLLKYEISSKGLRLDIQM 249

Query: 245 FFGFVGLFTVLGLWWLVWPLTAVGIEPPLKFPPSKSVTEVVLLNGFVGSVLSDYFWALSV 304
           F G+VG+FT L  W ++  L    +E   + P +  ++ +V+LN  +   +SDYFW  ++
Sbjct: 250 FLGYVGIFTFLLFWPILIILDITHME-TFELPSNFHISFLVMLNCII-IFVSDYFWCKAL 309

Query: 305 IWTSPLVATLGMSLTIPLAMLADVILHGRRYSALYILGCV 327
           I TSPLV T+ ++ TIPLAM AD +     ++  YI+G +
Sbjct: 310 ILTSPLVVTVALTFTIPLAMFADFVWREAFFTPWYIIGVI 338

BLAST of Sgr027850 vs. ExPASy Swiss-Prot
Match: Q8R314 (Solute carrier family 35 member F5 OS=Mus musculus OX=10090 GN=Slc35f5 PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 4.4e-28
Identity = 110/402 (27.36%), Postives = 182/402 (45.27%), Query Frame = 0

Query: 7   IGLGLICTAVVIWVTSAEITQRIFSEYKHPFALTYLGVSLMVVYL--------------- 66
           +G+ ++    VIWV S+E+T  +F++Y  PF  T+   S+ V+YL               
Sbjct: 71  LGIVILLLVDVIWVASSELTSYVFTQYNKPFFSTFAKTSMFVLYLLGFIIWKPWRQQCTR 130

Query: 67  -----PVAVVKD-----FVCSL---------------LNNHPDNDGSVLSSSIGPD---- 126
                P A   D       C+                +  H      + S++IG +    
Sbjct: 131 GFRGKPAAFFADAEGYFAACTTDTSMSSSLSEPLYVPVKFHDLPSEKLESTNIGTEKTPK 190

Query: 127 --------------------IPLRFNEVQYSLDEDMESCL-----ISDKDLSEREEYFS- 186
                               +  + + + Y   +D ES L     ++   +++   +F  
Sbjct: 191 KSRVRFSNIMEIRQLPSSHALEAKLSRMSYPTVKDQESILKTVGKLTATQVAKISFFFCF 250

Query: 187 ---------NSALANTSVASATILNSTSGLFALLFGALLGQES---ITISKVVAVFISMA 246
                      AL++T VA   IL+STSGLF L+  A+    S    T+SK++AV +S+ 
Sbjct: 251 VWFLANLSYQEALSDTQVAIVNILSSTSGLFTLILAAVFPSNSGDRFTLSKLLAVILSIG 310

Query: 247 GVAMTTLGKTWATNEFLIISDSRERTVTGDIFGLLSAVIYGLFTVLLKKSAGSEGDKIDV 306
           GV +  L  +           S  R   G I+ L  A+ Y ++ V++K+    E DK+D+
Sbjct: 311 GVVLVNLSGS---------EKSAGRDTIGSIWSLAGAMFYAVYIVMIKRKVDRE-DKLDI 370

Query: 307 QKFFGFVGLFTVLGLWWLVWPLTAVGIEPPLKFPPSKSVTEVVLLNGFVGSVLSDYFWAL 327
             FFGFVGLF +L LW   + L   G E   +F P+K V   +++NG +G+VLS++ W  
Sbjct: 371 PMFFGFVGLFNLLLLWPGFFLLHYTGFE-DFEF-PNKVVLLCIIINGLIGTVLSEFLWLW 430

BLAST of Sgr027850 vs. ExPASy TrEMBL
Match: A0A6J1CLJ1 (uncharacterized vacuolar membrane protein YML018C-like OS=Momordica charantia OX=3673 GN=LOC111012569 PE=3 SV=1)

HSP 1 Score: 546.2 bits (1406), Expect = 1.1e-151
Identity = 294/366 (80.33%), Postives = 308/366 (84.15%), Query Frame = 0

Query: 1   MGWSYRIGLGLICTAVVIWVTSAEITQRIFSEYKHPFALTYLGVSLMVVYLPVAVVKDFV 60
           MGW YRIGLGLI TAVVIWVTSAEITQRIFSEYKHPFAL+YLGVSLMVVYLPVAVV+D +
Sbjct: 1   MGWRYRIGLGLILTAVVIWVTSAEITQRIFSEYKHPFALSYLGVSLMVVYLPVAVVRDLI 60

Query: 61  CSLL-----NNHPDNDGSVLSSSIGPDIPLRFNEVQYSLDEDMESCLISDKDLSERE--- 120
            SLL     NNHPDN GSVLSSSIG D PLRFNE   SLDE M SCLISDKDLSERE   
Sbjct: 61  FSLLNPDLPNNHPDNGGSVLSSSIGLDFPLRFNEAHNSLDEGMGSCLISDKDLSEREEGQ 120

Query: 121 -------------------------------EYFSNSALANTSVASATILNSTSGLFALL 180
                                          EYFSNSALANTSVA+ATILNSTSGLFALL
Sbjct: 121 PLIPKIEPKNEVSSWEIAKCSLFLTPLWFTTEYFSNSALANTSVATATILNSTSGLFALL 180

Query: 181 FGALLGQESITISKVVAVFISMAGVAMTTLGKTWATNEFLIISDSRERTVTGDIFGLLSA 240
           FGA+LGQESITISKVVAV ISMAGVAMTTLGKTWATNE+LIIS+SR RTV GD+FGLLSA
Sbjct: 181 FGAVLGQESITISKVVAVLISMAGVAMTTLGKTWATNEYLIISESRGRTVMGDMFGLLSA 240

Query: 241 VIYGLFTVLLKKSAGSEGDKIDVQKFFGFVGLFTVLGLWWLVWPLTAVGIEPPLKFPPSK 300
           V YGLFTVLLKKSAGSEGDKIDVQKFFG+VGLFT+LGLWWLVWPLTAVGIEPPLKFP SK
Sbjct: 241 VTYGLFTVLLKKSAGSEGDKIDVQKFFGYVGLFTILGLWWLVWPLTAVGIEPPLKFPASK 300

Query: 301 SVTEVVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSLTIPLAMLADVILHGRRYSALY 328
           SVTE+VLLNGFVGSV+SDY WA SVIWTSPLVATLGMSLTIPLAMLADV+LHGRRYSALY
Sbjct: 301 SVTEIVLLNGFVGSVVSDYLWAHSVIWTSPLVATLGMSLTIPLAMLADVVLHGRRYSALY 360

BLAST of Sgr027850 vs. ExPASy TrEMBL
Match: A0A1S3BUT3 (uncharacterized transporter C405.03c-like OS=Cucumis melo OX=3656 GN=LOC103493733 PE=3 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 3.7e-147
Identity = 286/368 (77.72%), Postives = 309/368 (83.97%), Query Frame = 0

Query: 1   MGWSYRIGLGLICTAVVIWVTSAEITQRIFSEYKHPFALTYLGVSLMVVYLPVAVVKDFV 60
           MG  Y+IGLGLICTAV+IWV SAEITQRIFSEYKHPFAL+YLGVSLMV+YLPVAV+KD V
Sbjct: 1   MGCKYKIGLGLICTAVLIWVASAEITQRIFSEYKHPFALSYLGVSLMVIYLPVAVLKDLV 60

Query: 61  CSLLNNH---PDNDGSVLSSSIGPDIPLRFNEVQYSLDEDMESCL-ISDKDLS-ERE--- 120
           CSLLN H    +N GS++S+SIG D PLRFNE+ Y+LD  M  CL I+DKDLS ERE   
Sbjct: 61  CSLLNPHNLLDNNYGSIISTSIGIDGPLRFNEMHYNLDGSMGHCLIINDKDLSTEREELG 120

Query: 121 ---------------------------------EYFSNSALANTSVASATILNSTSGLFA 180
                                            EYFSNSALANTSVA+ATILNSTSGLFA
Sbjct: 121 QPLIPNFESSHHKVSTWEIIKCSLYLTPLWFTTEYFSNSALANTSVATATILNSTSGLFA 180

Query: 181 LLFGALLGQESITISKVVAVFISMAGVAMTTLGKTWATNEFLIISDSRERTVTGDIFGLL 240
           LLFGAL+GQESITISKVVAVFISMAGV MTTLGKTWATNEFLIIS+ R R++TGDIFGLL
Sbjct: 181 LLFGALVGQESITISKVVAVFISMAGVIMTTLGKTWATNEFLIISEPRGRSITGDIFGLL 240

Query: 241 SAVIYGLFTVLLKKSAGSEGDKIDVQKFFGFVGLFTVLGLWWLVWPLTAVGIEPPLKFPP 300
           SA IYGLFTVLLKKSAGSEGDKIDVQKFFG+VGLFT+LGLWWLVWPLTAVGIEPPLKFPP
Sbjct: 241 SAAIYGLFTVLLKKSAGSEGDKIDVQKFFGYVGLFTILGLWWLVWPLTAVGIEPPLKFPP 300

Query: 301 SKSVTEVVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSLTIPLAMLADVILHGRRYSA 328
           SKS+TE+VLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSLTIPLAMLADV+LHGRRYSA
Sbjct: 301 SKSITEIVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSLTIPLAMLADVVLHGRRYSA 360

BLAST of Sgr027850 vs. ExPASy TrEMBL
Match: A0A6J1IYL1 (thiamine-repressible mitochondrial transport protein THI74-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111481080 PE=3 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 8.0e-142
Identity = 271/367 (73.84%), Postives = 301/367 (82.02%), Query Frame = 0

Query: 1   MGWSYRIGLGLICTAVVIWVTSAEITQRIFSEYKHPFALTYLGVSLMVVYLPVAVVKDFV 60
           MG  Y+ GLGLIC AV+IWV SAEITQRIF+EYK PFAL+YLGVSLMVVYLPVAVVKD V
Sbjct: 1   MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV 60

Query: 61  CS-----LLNNHPDNDGSVLSSSIGPDIPLRFNEVQYSLDEDMESCLISDKDLSERE--- 120
            S     LLN+H +NDGSV SSSIG D+PL+FNE+ Y+ DE ++SCL SDKDLSERE   
Sbjct: 61  YSLLNPDLLNDHHENDGSVPSSSIGLDVPLKFNEIHYNFDEGLKSCLTSDKDLSEREEGQ 120

Query: 121 -------------------------------EYFSNSALANTSVASATILNSTSGLFALL 180
                                          EYFSNSALANTSVA+ATILNSTSGLFALL
Sbjct: 121 PLMSNFESHHKVSTWEIIERSLYLTPLWFTTEYFSNSALANTSVATATILNSTSGLFALL 180

Query: 181 FGALLGQESITISKVVAVFISMAGVAMTTLGKTWATNEFLIISDSRERTVTGDIFGLLSA 240
           FGAL+GQESITI+KVVAVFISMAGV MTTLGKTWATN F +IS+SR RT+TGDIF LLSA
Sbjct: 181 FGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESRGRTITGDIFCLLSA 240

Query: 241 VIYGLFTVLLKKSAGSEGDKIDVQKFFGFVGLFTVLGLWWLVWPLTAVGIEPPLKFPPSK 300
            IYGLFTVLLKK AGS GDKIDVQKFFG+VGLFT++GLWWLVWPLTA+GIEPP KFPPSK
Sbjct: 241 AIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSK 300

Query: 301 SVTEVVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSLTIPLAMLADVILHGRRYSALY 329
           S+TE V+LNGFVG+VLSDYFWALSVIWTSPLV+ LGMSLTIPLAMLAD++LHGRRYS LY
Sbjct: 301 SITESVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADIVLHGRRYSTLY 360

BLAST of Sgr027850 vs. ExPASy TrEMBL
Match: A0A6J1J463 (thiamine-repressible mitochondrial transport protein THI74-like isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111481080 PE=3 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 1.8e-141
Identity = 271/366 (74.04%), Postives = 300/366 (81.97%), Query Frame = 0

Query: 1   MGWSYRIGLGLICTAVVIWVTSAEITQRIFSEYKHPFALTYLGVSLMVVYLPVAVVKDFV 60
           MG  Y+ GLGLIC AV+IWV SAEITQRIF+EYK PFAL+YLGVSLMVVYLPVAVVKD V
Sbjct: 1   MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV 60

Query: 61  CS-----LLNNHPDNDGSVLSSSIGPDIPLRFNEVQYSLDEDMESCLISDKDLSERE--- 120
            S     LLN+H +NDGSV SSSIG D+PL+FNE+ Y+ DE ++SCL SDKDLSERE   
Sbjct: 61  YSLLNPDLLNDHHENDGSVPSSSIGLDVPLKFNEIHYNFDEGLKSCLTSDKDLSEREEGQ 120

Query: 121 -------------------------------EYFSNSALANTSVASATILNSTSGLFALL 180
                                          EYFSNSALANTSVA+ATILNSTSGLFALL
Sbjct: 121 PLMSNFESHHKVSTWEIIERSLYLTPLWFTTEYFSNSALANTSVATATILNSTSGLFALL 180

Query: 181 FGALLGQESITISKVVAVFISMAGVAMTTLGKTWATNEFLIISDSRERTVTGDIFGLLSA 240
           FGAL+GQESITI+KVVAVFISMAGV MTTLGKTWATN F +IS+SR RT+TGDIF LLSA
Sbjct: 181 FGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESRGRTITGDIFCLLSA 240

Query: 241 VIYGLFTVLLKKSAGSEGDKIDVQKFFGFVGLFTVLGLWWLVWPLTAVGIEPPLKFPPSK 300
            IYGLFTVLLKK AGS GDKIDVQKFFG+VGLFT++GLWWLVWPLTA+GIEPP KFPPSK
Sbjct: 241 AIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSK 300

Query: 301 SVTEVVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSLTIPLAMLADVILHGRRYSALY 328
           S+TE V+LNGFVG+VLSDYFWALSVIWTSPLV+ LGMSLTIPLAMLAD++LHGRRYS LY
Sbjct: 301 SITESVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADIVLHGRRYSTLY 360

BLAST of Sgr027850 vs. ExPASy TrEMBL
Match: A0A6J1FHK5 (thiamine-repressible mitochondrial transport protein THI74-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445844 PE=3 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 7.5e-140
Identity = 267/367 (72.75%), Postives = 300/367 (81.74%), Query Frame = 0

Query: 1   MGWSYRIGLGLICTAVVIWVTSAEITQRIFSEYKHPFALTYLGVSLMVVYLPVAVVKDFV 60
           MG  Y+ GLGLIC AV+IWV SAEITQRIF+EYK PFAL+YLGVSLMVVYLPVAVVKD V
Sbjct: 1   MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV 60

Query: 61  CS-----LLNNHPDNDGSVLSSSIGPDIPLRFNEVQYSLDEDMESCLISDKDLSERE--- 120
            S     LLN+H +NDGSV SSSIG  +PL+FNE+ Y+ DED++S L SDKDLSE E   
Sbjct: 61  YSLLNPDLLNDHHENDGSVPSSSIGLGVPLKFNEIHYNFDEDLKSSLTSDKDLSEEEEGQ 120

Query: 121 -------------------------------EYFSNSALANTSVASATILNSTSGLFALL 180
                                          EYFSNSALANTSVA+ATILNSTSGLFALL
Sbjct: 121 PLMSNFESHHKVSTREIIERSLYLTPIWFTTEYFSNSALANTSVATATILNSTSGLFALL 180

Query: 181 FGALLGQESITISKVVAVFISMAGVAMTTLGKTWATNEFLIISDSRERTVTGDIFGLLSA 240
           FGAL+GQESITI+KVVAVFISMAGVAMTTLGKTWATN F +I +SR RT+TGD+F LLSA
Sbjct: 181 FGALVGQESITITKVVAVFISMAGVAMTTLGKTWATNGFPVIIESRGRTITGDVFCLLSA 240

Query: 241 VIYGLFTVLLKKSAGSEGDKIDVQKFFGFVGLFTVLGLWWLVWPLTAVGIEPPLKFPPSK 300
            IYGLFTVLLKK AGS GDKIDVQKFFG+VGLFT++GLWWLVWPLTA+GIEPP KFPPSK
Sbjct: 241 AIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSK 300

Query: 301 SVTEVVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSLTIPLAMLADVILHGRRYSALY 329
           S+TE+V+LNGFVG+VLSDYFWALSVIWTSPLV+ LGMSLTIPLAMLAD++LHGRRYS +Y
Sbjct: 301 SITEIVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADMVLHGRRYSTIY 360

BLAST of Sgr027850 vs. TAIR 10
Match: AT4G32140.1 (EamA-like transporter family )

HSP 1 Score: 375.2 bits (962), Expect = 6.5e-104
Identity = 206/374 (55.08%), Postives = 254/374 (67.91%), Query Frame = 0

Query: 1   MGWSYRIGLGLICTAVVIWVTSAEITQRIFSEYKHPFALTYLGVSLMVVYLPVAVVKDFV 60
           MGW Y+ GL LI T V+IWVTSAE+TQ IF+ YK PFA+TYLG SLM+VYLPVA +KD++
Sbjct: 1   MGWRYKAGLFLIGTVVIIWVTSAEVTQDIFTAYKQPFAVTYLGASLMIVYLPVAFLKDWL 60

Query: 61  CSLLNNHPDNDGSVLS----SSIGPDIPLRFNEVQYSLD-----EDMESCLISDKDLSER 120
           C  L+        + +    SS+    PLR   ++  L      +D E  L S ++  ER
Sbjct: 61  CRYLDRRSSKSNKIPALTDDSSVELGSPLRHKIIEMGLQGTITKKDSEEHLSSHEEEDER 120

Query: 121 -------------------------------------EEYFSNSALANTSVASATILNST 180
                                                 EY SN+ALA TSVAS T+L+ST
Sbjct: 121 PLIGKVREEVQTLKQRKEITTKQIAMYGLYLAPIWFVTEYLSNAALARTSVASTTVLSST 180

Query: 181 SGLFALLFGALLGQESITISKVVAVFISMAGVAMTTLGKTWATNEFLIISD-SRERTVTG 240
           SGLF L  G  LGQ+++ +SKVVAVF+SMAGV MTTLGKTWA++E  + S  + ER++ G
Sbjct: 181 SGLFTLFIGVFLGQDTLNLSKVVAVFVSMAGVVMTTLGKTWASDESQLNSSLNGERSLMG 240

Query: 241 DIFGLLSAVIYGLFTVLLKKSAGSEGDKIDVQKFFGFVGLFTVLGLWWLVWPLTAVGIEP 300
           D+FGLLSAV YGLFTVLLKK AG EG+ +DVQK FG++GLFT++ LWWLVWPLTA+GIEP
Sbjct: 241 DLFGLLSAVSYGLFTVLLKKFAGEEGEGVDVQKLFGYIGLFTLVALWWLVWPLTALGIEP 300

Query: 301 PLKFPPSKSVTEVVLLNGFVGSVLSDYFWALSVIWTSPLVATLGMSLTIPLAMLADVILH 328
               P S  V EVVL NGF+GSVLSDYFWALSV+WT+PLVATLGMSLTIPLAM+AD+++H
Sbjct: 301 KFTIPHSVKVDEVVLANGFIGSVLSDYFWALSVVWTTPLVATLGMSLTIPLAMVADMMIH 360

BLAST of Sgr027850 vs. TAIR 10
Match: AT3G07080.1 (EamA-like transporter family )

HSP 1 Score: 78.6 bits (192), Expect = 1.3e-14
Identity = 56/197 (28.43%), Postives = 103/197 (52.28%), Query Frame = 0

Query: 117 NSALANTSVASATILNSTSGLFALLFGALLGQESITISKVVAVFISMAGVAMTTLGKTWA 176
           N +L  T+V S TIL+S S LF  L   +   E  T  K+ +V + M+G  + ++G + +
Sbjct: 182 NVSLKYTTVTSNTILSSASSLFTFLVSLIFLGEKFTWLKLFSVLLCMSGTIIVSMGDSES 241

Query: 177 TNEFLIISDSRERTVTGDIFGLLSAVIYGLFTVLLKKSAGSEGD---KIDVQKFFGFVGL 236
            +  +      +  + GDI  L+SA +Y ++  L++K    + +   ++ + +F GF+GL
Sbjct: 242 KSNAV-----AKNPLLGDILSLVSAALYAVYITLIRKKLPDDDERNGRVSMAQFLGFLGL 301

Query: 237 FTVLGLWWLVWPLTAVGIEPPLKFPP-SKSVTEVVLLNGFVGSVLSDYFWALSVIWTSPL 296
           F     +  + P   +      +F   +     +V+  G + +VLSDY WA +V+ T+  
Sbjct: 302 F---NFFIFLPPALILNFTKRERFNALTLKQFGLVVGKGLLDNVLSDYLWAKAVLLTTTT 361

Query: 297 VATLGMSLTIPLAMLAD 310
           VAT G+++ +PLA + D
Sbjct: 362 VATAGLTIQVPLAAIVD 370

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022142439.12.3e-15180.33uncharacterized vacuolar membrane protein YML018C-like [Momordica charantia][more]
XP_038899552.11.5e-14777.20uncharacterized vacuolar membrane protein YML018C-like [Benincasa hispida][more]
XP_008452823.17.7e-14777.72PREDICTED: uncharacterized transporter C405.03c-like [Cucumis melo][more]
XP_004145580.18.5e-14677.72uncharacterized transporter C405.03c [Cucumis sativus][more]
XP_023524239.17.9e-14474.86thiamine-repressible mitochondrial transport protein THI74-like isoform X1 [Cucu... [more]
Match NameE-valueIdentityDescription
Q037305.3e-3432.75Uncharacterized vacuolar membrane protein YML018C OS=Saccharomyces cerevisiae (s... [more]
O946548.0e-3030.18Uncharacterized transporter C405.03c OS=Schizosaccharomyces pombe (strain 972 / ... [more]
A6QL921.5e-2827.11Solute carrier family 35 member F5 OS=Bos taurus OX=9913 GN=SLC35F5 PE=2 SV=1[more]
Q040832.0e-2830.29Thiamine-repressible mitochondrial transport protein THI74 OS=Saccharomyces cere... [more]
Q8R3144.4e-2827.36Solute carrier family 35 member F5 OS=Mus musculus OX=10090 GN=Slc35f5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CLJ11.1e-15180.33uncharacterized vacuolar membrane protein YML018C-like OS=Momordica charantia OX... [more]
A0A1S3BUT33.7e-14777.72uncharacterized transporter C405.03c-like OS=Cucumis melo OX=3656 GN=LOC10349373... [more]
A0A6J1IYL18.0e-14273.84thiamine-repressible mitochondrial transport protein THI74-like isoform X3 OS=Cu... [more]
A0A6J1J4631.8e-14174.04thiamine-repressible mitochondrial transport protein THI74-like isoform X4 OS=Cu... [more]
A0A6J1FHK57.5e-14072.75thiamine-repressible mitochondrial transport protein THI74-like isoform X1 OS=Cu... [more]
Match NameE-valueIdentityDescription
AT4G32140.16.5e-10455.08EamA-like transporter family [more]
AT3G07080.11.3e-1428.43EamA-like transporter family [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 115..170
e-value: 3.3E-7
score: 30.5
NoneNo IPR availablePANTHERPTHR23051SOLUTE CARRIER FAMILY 35, MEMBER F5coord: 112..327
NoneNo IPR availablePANTHERPTHR23051SOLUTE CARRIER FAMILY 35, MEMBER F5coord: 1..113
NoneNo IPR availablePANTHERPTHR23051:SF9THIAMINE-REPRESSIBLE MITOCHONDRIAL TRANSPORT PROTEIN THI74-LIKE ISOFORM X1coord: 112..327
coord: 1..113
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 115..173

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr027850.1Sgr027850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane