Sgr027318 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr027318 vs. NCBI nr
Match: XP_022987083.1 (subtilisin-like protease SBT1.8 [Cucurbita maxima])

HSP 1 Score: 1352.8 bits (3500), Expect = 0.0e+00
Identity = 683/756 (90.34%), Postives = 705/756 (93.25%), Query Frame = 0

Query: 1   MGSMARLLIAS---LLLLLPCISVNAKRTYIVRMKHHALPSDYSTHHDWYSAHLQSLSSS 60
           MGSMARLL+AS   LLLLLPC+ VNAKRTYIVRMKHHALPSDY THHDWYSAHLQSLSSS
Sbjct: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60

Query: 61  ITSDSLLYTYTSAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYDLHTTRTPGFLGLDSN 120
            TSDSLLYTYTSAYHGFAASLDP+EAELLRQSDSVLGVYEDTVY LHTTRTPGFLGLDS+
Sbjct: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYKLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGVWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180
           FGLWEGHNTQDLDQASHDVIIGVLDTG+WPESKSFDDTGMPEIPSRWRGECESGPDFSPS
Sbjct: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYFRK RENESPRDQDGHGTHTASTAAGSHVANASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILA--------------------------- 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILA                           
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 --AIGAFSAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
             AIGAF+AMEKGVFVSCSAGNSGPN+ASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 FTGVSLYSGQGMGNKPVALVYNKGSNTSSNMCLPGSLEPAIVRGKVVVCDRGINARVEKG 420
           FTGVSLYSGQGMGNKPVALVYNKGSN+SSNMCLPGSLEPA VRGKVV+CDRGINARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYVRSV+KPTAVLSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480

Query: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQ 540
           GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQ
Sbjct: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
           FNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGMFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLLYDLSTDDYIAFLCSLDYGIDHVQAIVKRPNVTCSRMFAD 660
           NPWAHGAGHVDPHKALSPGLLYD+ST+DYI FLCSLDYGIDHVQAIVKR N+TCS+ FAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLLYDISTNDYITFLCSLDYGIDHVQAIVKRSNITCSKKFAD 660

Query: 661 PGQLNYPSFSVVFGSKRVVRYTREVTNVGPTGSVYEVATTSPSVVKVMVKPTKLVFTKVG 720
           PGQLNYPSFSVVFGSKRVVRYTR VTNVG  GSVYEVATT+PSVVKV VKP+KLVF+KVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720

Query: 721 EKKRYTVTFTASSDAAQTTRFGFGSIAWSNDQHQFK 725
           E+KRYTVTF AS DAA+TTR+GFGSIAWSNDQHQ +
Sbjct: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVR 756

BLAST of Sgr027318 vs. NCBI nr
Match: KAG6590040.1 (Subtilisin-like protease 1.8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1350.9 bits (3495), Expect = 0.0e+00
Identity = 681/755 (90.20%), Postives = 705/755 (93.38%), Query Frame = 0

Query: 1   MGSMARLLIAS--LLLLLPCISVNAKRTYIVRMKHHALPSDYSTHHDWYSAHLQSLSSSI 60
           MGSMARLL+AS  LLLLLPC+ VNAKRTYIVRMKHHALPSDY THHDWYSAHLQSLSSS 
Sbjct: 1   MGSMARLLVASFLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSSA 60

Query: 61  TSDSLLYTYTSAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYDLHTTRTPGFLGLDSNF 120
           TSDSLLYTYTSAYHGFAASLDP+EAELLRQSDSVLGVYEDTVY+LHTTRTPGFLGLDS+F
Sbjct: 61  TSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120

Query: 121 GLWEGHNTQDLDQASHDVIIGVLDTGVWPESKSFDDTGMPEIPSRWRGECESGPDFSPSL 180
           GLWEGHNTQDLDQASHDVIIGVLDTG+WPESKSFDDTGMPEIPSRWRGECESGPDFSPSL
Sbjct: 121 GLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSL 180

Query: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLGY 240
           CNKKLIGARSFSKGYQMASGGGYFRK RENESPRDQDGHGTHTASTAAGSHVANASLLGY
Sbjct: 181 CNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLGY 240

Query: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILA---------------------------- 300
           ARGIARGMAPQARVAAYKTCWPTGCFGSDILA                            
Sbjct: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300

Query: 301 -AIGAFSAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
            AIGAF+AMEKGVFVSCSAGNSGPN+ASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF
Sbjct: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360

Query: 361 TGVSLYSGQGMGNKPVALVYNKGSNTSSNMCLPGSLEPAIVRGKVVVCDRGINARVEKGG 420
           TGVSLYSGQGMGNKPVALVYNKGSN+SSNMCLPGSLEPA VRGKVV+CDRGINARVEKGG
Sbjct: 361 TGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKGG 420

Query: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFGG 480
           VVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYVRSV+KPTAVLSFGG
Sbjct: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFGG 480

Query: 481 TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQF 540
           TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQF
Sbjct: 481 TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQF 540

Query: 541 NIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSN 600
           NIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSN
Sbjct: 541 NIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSN 600

Query: 601 PWAHGAGHVDPHKALSPGLLYDLSTDDYIAFLCSLDYGIDHVQAIVKRPNVTCSRMFADP 660
           PWAHGAGHVDPHKALSPGL+YD+ST+DYI FLCSLDYGIDHVQAI KR N+TC + FADP
Sbjct: 601 PWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFADP 660

Query: 661 GQLNYPSFSVVFGSKRVVRYTREVTNVGPTGSVYEVATTSPSVVKVMVKPTKLVFTKVGE 720
           GQLNYPSFSVVFGSKRVVRYTR VTNVG  GSVYEVATT+PSVVKV VKP+KLVF+KVGE
Sbjct: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVGE 720

Query: 721 KKRYTVTFTASSDAAQTTRFGFGSIAWSNDQHQFK 725
           +KRYTVTF AS DAA+TTR+GFGSIAWSNDQHQ +
Sbjct: 721 RKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVR 755

BLAST of Sgr027318 vs. NCBI nr
Match: XP_022960792.1 (subtilisin-like protease SBT1.8 [Cucurbita moschata] >KAG7023704.1 Subtilisin-like protease SBT1.8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1350.5 bits (3494), Expect = 0.0e+00
Identity = 681/756 (90.08%), Postives = 705/756 (93.25%), Query Frame = 0

Query: 1   MGSMARLLIAS---LLLLLPCISVNAKRTYIVRMKHHALPSDYSTHHDWYSAHLQSLSSS 60
           MGSMARLL+AS   LLLLLPC+ VNAKRTYIVRMKHHALPSDY THHDWYSAHLQSLSSS
Sbjct: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60

Query: 61  ITSDSLLYTYTSAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYDLHTTRTPGFLGLDSN 120
            TSDSLLYTYTSAYHGFAASLDP+EAELLRQSDSVLGVYEDTVY+LHTTRTPGFLGLDS+
Sbjct: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGVWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180
           FGLWEGHNTQDLDQASHDVIIGVLDTG+WPESKSFDDTGMPEIPSRWRGECESGPDFSPS
Sbjct: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYFRK RENESPRDQDGHGTHTASTAAGSHVANASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILA--------------------------- 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILA                           
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 --AIGAFSAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
             AIGAF+AMEKGVFVSCSAGNSGPN+ASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 FTGVSLYSGQGMGNKPVALVYNKGSNTSSNMCLPGSLEPAIVRGKVVVCDRGINARVEKG 420
           FTGVSLYSGQGMGNKPVALVYNKGSN+SSNMCLPGSLEPA VRGKVV+CDRGINARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYVRSV+KPTAVLSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480

Query: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQ 540
           GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQ
Sbjct: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
           FNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLLYDLSTDDYIAFLCSLDYGIDHVQAIVKRPNVTCSRMFAD 660
           NPWAHGAGHVDPHKALSPGL+YD+ST+DYI FLCSLDYGIDHVQAI KR N+TC + FAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFAD 660

Query: 661 PGQLNYPSFSVVFGSKRVVRYTREVTNVGPTGSVYEVATTSPSVVKVMVKPTKLVFTKVG 720
           PGQLNYPSFSVVFGSKRVVRYTR VTNVG  GSVYEVATT+PSVVKV VKP+KLVF+KVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720

Query: 721 EKKRYTVTFTASSDAAQTTRFGFGSIAWSNDQHQFK 725
           E+KRYTVTF AS DAA+TTR+GFGSIAWSNDQHQ +
Sbjct: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVR 756

BLAST of Sgr027318 vs. NCBI nr
Match: XP_023516159.1 (subtilisin-like protease SBT1.8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1346.6 bits (3484), Expect = 0.0e+00
Identity = 679/755 (89.93%), Postives = 703/755 (93.11%), Query Frame = 0

Query: 1   MGSMARLLIA--SLLLLLPCISVNAKRTYIVRMKHHALPSDYSTHHDWYSAHLQSLSSSI 60
           MGSMA LL+A   LLLLLPC+ VNAKRTYIVRMKHHALPSDY THHDWYSAHLQSLSSS 
Sbjct: 1   MGSMASLLVAYFLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSSA 60

Query: 61  TSDSLLYTYTSAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYDLHTTRTPGFLGLDSNF 120
           TSDSLLYTYTSAYHGFAASLDP+EAELLRQSDSVLGVYEDTVY+LHTTRTPGFLGLDS+F
Sbjct: 61  TSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120

Query: 121 GLWEGHNTQDLDQASHDVIIGVLDTGVWPESKSFDDTGMPEIPSRWRGECESGPDFSPSL 180
           GLWEGHNTQDLDQASHDVIIGVLDTG+WPESKSFDDTGMPEIPSRWRGECESGPDFSPSL
Sbjct: 121 GLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPSL 180

Query: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLGY 240
           CNKKLIGARSFSKGYQMASGGGYFRK RENESPRDQDGHGTHTASTAAGSHVANASLLGY
Sbjct: 181 CNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLGY 240

Query: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILA---------------------------- 300
           ARGIARGMAPQARVAAYKTCWPTGCFGSDILA                            
Sbjct: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300

Query: 301 -AIGAFSAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
            AIGAF+AMEKGVFVSCSAGNSGPN+ASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF
Sbjct: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360

Query: 361 TGVSLYSGQGMGNKPVALVYNKGSNTSSNMCLPGSLEPAIVRGKVVVCDRGINARVEKGG 420
           TGVSLYSGQGMGNKPVALVYNKGSN+SSNMCLPGSLEPA VRGKVV+CDRGINARVEKGG
Sbjct: 361 TGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKGG 420

Query: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFGG 480
           VVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYVRSV+KPTAVLSFGG
Sbjct: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFGG 480

Query: 481 TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQF 540
           TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQF
Sbjct: 481 TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQF 540

Query: 541 NIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSN 600
           NIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FSN
Sbjct: 541 NIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGMFSN 600

Query: 601 PWAHGAGHVDPHKALSPGLLYDLSTDDYIAFLCSLDYGIDHVQAIVKRPNVTCSRMFADP 660
           PWAHGAGHVDPHKALSPGL+YD+ST+DYI FLCSLDYGIDHVQAI KR N+TCS+ FADP
Sbjct: 601 PWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCSKKFADP 660

Query: 661 GQLNYPSFSVVFGSKRVVRYTREVTNVGPTGSVYEVATTSPSVVKVMVKPTKLVFTKVGE 720
           GQLNYPSFSVVFGSKRVVRYTR VTNVG  GSVYEVATT+PSVVKV VKP+KLVF+KVGE
Sbjct: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVGE 720

Query: 721 KKRYTVTFTASSDAAQTTRFGFGSIAWSNDQHQFK 725
           +KRYTVTF AS DAA+TTR+GFGSIAWSNDQHQ +
Sbjct: 721 RKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVR 755

BLAST of Sgr027318 vs. NCBI nr
Match: XP_038879224.1 (subtilisin-like protease SBT1.8 [Benincasa hispida])

HSP 1 Score: 1338.2 bits (3462), Expect = 0.0e+00
Identity = 676/753 (89.77%), Postives = 700/753 (92.96%), Query Frame = 0

Query: 1   MGSMARLLIASLLLLLPCISVNAKRTYIVRMKHHALPSDYSTHHDWYSAHLQSLSSSITS 60
           MGSMARLLIA LLLLLPC+ VNAK+TYIVRMKH+ALPS+Y THHDWYSAHLQSLSSS TS
Sbjct: 1   MGSMARLLIAFLLLLLPCVFVNAKKTYIVRMKHNALPSEYLTHHDWYSAHLQSLSSSSTS 60

Query: 61  DSLLYTYTSAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYDLHTTRTPGFLGLDSNFGL 120
           DSLLYTYTSAYHGFAASLD  EAELLRQSDSVLGVYED+VY LHTTRTPGFLGLDS+FGL
Sbjct: 61  DSLLYTYTSAYHGFAASLDSNEAELLRQSDSVLGVYEDSVYTLHTTRTPGFLGLDSDFGL 120

Query: 121 WEGHNTQDLDQASHDVIIGVLDTGVWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCN 180
           WEGHNTQDL+QASHDVIIGVLDTG+WPESKSFDDTGMPEIPSRWRGECESG DFSPSLCN
Sbjct: 121 WEGHNTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGADFSPSLCN 180

Query: 181 KKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLGYAR 240
           KKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLGYAR
Sbjct: 181 KKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLGYAR 240

Query: 241 GIARGMAPQARVAAYKTCWPTGCFGSDILA-----------------------------A 300
           GIARGMAPQARVAAYKTCWPTGCFGSDILA                             A
Sbjct: 241 GIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIA 300

Query: 301 IGAFSAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTG 360
           IGAF+AMEKGVFVSCSAGNSGPN+ASLANVAPWIMTVGAGTLDRDFPAYVQ+GNGKRFTG
Sbjct: 301 IGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQIGNGKRFTG 360

Query: 361 VSLYSGQGMGNKPVALVYNKGSNTSSNMCLPGSLEPAIVRGKVVVCDRGINARVEKGGVV 420
           VSLYSGQGMG+K V LVYNKGSNTSSNMCLPGSLEPA+VRGKVVVCDRGINARVEKGGVV
Sbjct: 361 VSLYSGQGMGSKAVELVYNKGSNTSSNMCLPGSLEPAVVRGKVVVCDRGINARVEKGGVV 420

Query: 421 RDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFGGTV 480
           RDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRS S PTAVLSFGGT+
Sbjct: 421 RDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGGTI 480

Query: 481 LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNI 540
           LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQFNI
Sbjct: 481 LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQFNI 540

Query: 541 MSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPW 600
           MSGTSMSCPHISGLAALLKAAHP+WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FSNPW
Sbjct: 541 MSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNPW 600

Query: 601 AHGAGHVDPHKALSPGLLYDLSTDDYIAFLCSLDYGIDHVQAIVKRPNVTCSRMFADPGQ 660
           AHGAGHVDPHKALSPGLLYD+ST+DYIAFLCSLDYGIDHVQAIVKR N+TCSR FADPGQ
Sbjct: 601 AHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADPGQ 660

Query: 661 LNYPSFSVVFGSKRVVRYTREVTNVGPTGSVYEVATTSPSVVKVMVKPTKLVFTKVGEKK 720
           LNYPSFSVVFGSKRVVRYTR VTNVG  GSVYEVATT+PSVVKV VKP+KLVFTKVGE+K
Sbjct: 661 LNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFTKVGERK 720

Query: 721 RYTVTFTASSDAAQTTRFGFGSIAWSNDQHQFK 725
           RYTVTF AS DAAQT RFGFGSIAW+N QHQ +
Sbjct: 721 RYTVTFVASRDAAQTMRFGFGSIAWNNYQHQVR 753

BLAST of Sgr027318 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 1014.2 bits (2621), Expect = 1.3e-294
Identity = 515/747 (68.94%), Postives = 596/747 (79.79%), Query Frame = 0

Query: 8   LIASLLLLLPCISVNAKRTYIVRMKHHALPSDYSTHHDWYSAHLQSLSSSITSDSLLYTY 67
           +I + L LL  +   AK+TYI+R+ H   P  + THHDWY++ L S S      SLLYTY
Sbjct: 13  IITTFLFLL--LHTTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNSES------SLLYTY 72

Query: 68  TSAYHGFAASLDPEEAE-LLRQSDSVLGVYEDTVYDLHTTRTPGFLGLDSNFGLWEGHNT 127
           T+++HGF+A LD  EA+ LL  S+S+L ++ED +Y LHTTRTP FLGL+S FG+      
Sbjct: 73  TTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGV------ 132

Query: 128 QDLDQASHDVIIGVLDTGVWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGA 187
            DL  +S+ VIIGVLDTGVWPES+SFDDT MPEIPS+W+GECESG DF   LCNKKLIGA
Sbjct: 133 HDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGA 192

Query: 188 RSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGM 247
           RSFSKG+QMASGGG+  K RE+ SPRD DGHGTHT++TAAGS V NAS LGYA G ARGM
Sbjct: 193 RSFSKGFQMASGGGFSSK-RESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGM 252

Query: 248 APQARVAAYKTCWPTGCFGSDILA-----------------------------AIGAFSA 307
           A +ARVA YK CW TGCFGSDILA                             AIGAFSA
Sbjct: 253 ATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSA 312

Query: 308 MEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSG 367
           ME+GVFVSCSAGNSGP RAS+ANVAPW+MTVGAGTLDRDFPA+  LGNGKR TGVSLYSG
Sbjct: 313 MERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSG 372

Query: 368 QGMGNKPVALVYNKGSNTSSNMCLPGSLEPAIVRGKVVVCDRGINARVEKGGVVRDAGGI 427
            GMG KP+ LVYNKG+++SSN+CLPGSL+ +IVRGK+VVCDRG+NARVEKG VVRDAGG+
Sbjct: 373 VGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGL 432

Query: 428 GMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFGGTVLNVRPS 487
           GMI+ANTAASGEELVADSHLLPA+AVG+KTGDL+R+YV+S SKPTA+L F GTVL+V+PS
Sbjct: 433 GMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPS 492

Query: 488 PVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSM 547
           PVVAAFSSRGPN VTP+ILKPDVIGPGVNILA WS++IGPTGLD D R+TQFNIMSGTSM
Sbjct: 493 PVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSM 552

Query: 548 SCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGH 607
           SCPHISGLA LLKAAHPEWSPSAIKSALMTTAY  DNTN+ L DAA  + SNP+AHG+GH
Sbjct: 553 SCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGH 612

Query: 608 VDPHKALSPGLLYDLSTDDYIAFLCSLDYGIDHVQAIVKRPNVTCSRMFADPGQLNYPSF 667
           VDP KALSPGL+YD+ST++YI FLCSLDY +DH+ AIVKRP+V CS+ F+DPGQLNYPSF
Sbjct: 613 VDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSF 672

Query: 668 SVVFGSKRVVRYTREVTNVGPTGSVYEVATTSPSVVKVMVKPTKLVFTKVGEKKRYTVTF 725
           SV+FG KRVVRYTREVTNVG   SVY+V       V + VKP+KL F  VGEKKRYTVTF
Sbjct: 673 SVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTF 732

BLAST of Sgr027318 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 742.7 bits (1916), Expect = 7.4e-213
Identity = 407/757 (53.76%), Postives = 512/757 (67.64%), Query Frame = 0

Query: 1   MGSMARLLIASLLLLLPCISVNAKRTYIVRMKHHALPSDYSTHHDWYSAHLQSLSSSITS 60
           + S A  L+  L       S + + TYIV M    +PS +  H +WY + L+S+S S   
Sbjct: 6   LSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA-- 65

Query: 61  DSLLYTYTSAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYDLHTTRTPGFLGLDSNFGL 120
             LLYTY +A HGF+  L  EEA+ L     V+ V  +  Y+LHTTRTP FLGLD     
Sbjct: 66  -ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLD----- 125

Query: 121 WEGHNTQDL--DQASH-DVIIGVLDTGVWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180
               +T DL  +  S+ DV++GVLDTGVWPESKS+ D G   IPS W+G CE+G +F+ S
Sbjct: 126 ---EHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTAS 185

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLG 240
           LCN+KLIGAR F++GY+  S  G   + +E+ SPRD DGHGTHT+STAAGS V  ASLLG
Sbjct: 186 LCNRKLIGARFFARGYE--STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLG 245

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILA--------------------------- 300
           YA G ARGMAP+ARVA YK CW  GCF SDILA                           
Sbjct: 246 YASGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRD 305

Query: 301 --AIGAFSAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
             AIGAF+AME+G+ VSCSAGN+GP+ +SL+NVAPWI TVGAGTLDRDFPA   LGNGK 
Sbjct: 306 GVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKN 365

Query: 361 FTGVSLYSGQGMGNKPVALVY--NKGSNTSSNMCLPGSLEPAIVRGKVVVCDRGINARVE 420
           FTGVSL+ G+ + +K +  +Y  N  + T+ N+C+ G+L P  V+GK+V+CDRGINARV+
Sbjct: 366 FTGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQ 425

Query: 421 KGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLS 480
           KG VV+ AGG+GMILANTAA+GEELVAD+HLLPA  VG K GD+IR YV +   PTA +S
Sbjct: 426 KGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASIS 485

Query: 481 FGGTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRK 540
             GTV+ V+PSPVVAAFSSRGPN +TP ILKPD+I PGVNILAAW+ + GPTGL SD R+
Sbjct: 486 ILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRR 545

Query: 541 TQFNIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGA 600
            +FNI+SGTSMSCPH+SGLAALLK+ HPEWSP+AI+SALMTTAY        L D A G 
Sbjct: 546 VEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGK 605

Query: 601 FSNPWAHGAGHVDPHKALSPGLLYDLSTDDYIAFLCSLDYGIDHVQAIVKRPNVTC--SR 660
            S P+ HGAGHV P  A +PGL+YDL+T+DY+ FLC+L+Y    ++++ +R N TC  S+
Sbjct: 606 PSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRR-NYTCDPSK 665

Query: 661 MFADPGQLNYPSFSVVFGSKRVVRYTREVTNVGPTGSVYEVATTSPSVVKVMVKPTKLVF 720
            ++    LNYPSF+V        +YTR VT+VG  G+     T+  + VK+ V+P  L F
Sbjct: 666 SYS-VADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNF 725

Query: 721 TKVGEKKRYTVTFTASSDAAQTTRFGFGSIAWSNDQH 722
            +  EKK YTVTFT  S     +   FGSI WS+ +H
Sbjct: 726 KEANEKKSYTVTFTVDSSKPSGSN-SFGSIEWSDGKH 746

BLAST of Sgr027318 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 9.1e-203
Identity = 388/770 (50.39%), Postives = 494/770 (64.16%), Query Frame = 0

Query: 4   MARLLIASLLLLLPCI---------SVNAKRTYIVRMKHHALPSDYSTHHDWYSAHLQSL 63
           MA+L ++S+  + P +         S +   +YIV ++    PS +S+H++W+ + L+SL
Sbjct: 1   MAKLSLSSIFFVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSL 60

Query: 64  SSSITSDSLLYTYTSAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYDLHTTRTPGFLGL 123
            SS    +LLY+Y+ A HGF+A L P +   LR+  SV+ V  D   ++HTT TP FLG 
Sbjct: 61  PSSPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGF 120

Query: 124 DSNFGLWEGHNTQDLDQASHDVIIGVLDTGVWPESKSFDDTGMPEIPSRWRGECESGPDF 183
             N GLW   N         DVI+GVLDTG+WPE  SF D+G+  IPS W+GECE GPDF
Sbjct: 121 SQNSGLWSNSN------YGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDF 180

Query: 184 SPSLCNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANAS 243
             S CN+KLIGAR+F +GY     G      +E+ SPRD +GHGTHTASTAAGS VANAS
Sbjct: 181 PASSCNRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANAS 240

Query: 244 LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILA------------------------ 303
           L  YARG A GMA +AR+AAYK CW  GC+ SDILA                        
Sbjct: 241 LYQYARGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAP 300

Query: 304 -------AIGAFSAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQL 363
                  AIGAF A   G+ VSCSAGNSGPN  +  N+APWI+TVGA T+DR+F A    
Sbjct: 301 EYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAIT 360

Query: 364 GNGKRFTGVSLYSGQGMGNKPVALVYNKGSNTSSNMCLPGSLEPAIVRGKVVVCDRGINA 423
           G+GK FTG SLY+G+ + +  ++LVY+   +  S +C PG L  ++V GK+V+CDRG NA
Sbjct: 361 GDGKVFTGTSLYAGESLPDSQLSLVYS--GDCGSRLCYPGKLNSSLVEGKIVLCDRGGNA 420

Query: 424 RVEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTA 483
           RVEKG  V+ AGG GMILANTA SGEEL ADSHL+PA  VG K GD IR Y+++   PTA
Sbjct: 421 RVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTA 480

Query: 484 VLSFGGTVLNVR-PSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDS 543
            +SF GT++    PSP VAAFSSRGPN +TP ILKPDVI PGVNILA W+  +GPT LD 
Sbjct: 481 KISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDI 540

Query: 544 DKRKTQFNIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDA 603
           D R+ QFNI+SGTSMSCPH+SGLAALL+ AHP+WSP+AIKSAL+TTAY  +N+   + D 
Sbjct: 541 DPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDL 600

Query: 604 AGGAFSNPWAHGAGHVDPHKALSPGLLYDLSTDDYIAFLCSLDYGIDHVQAIVKRPNV-- 663
           A G  SN + HGAGHVDP+KAL+PGL+YD+   +Y+AFLC++ Y    +   ++ P +  
Sbjct: 601 ATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYD 660

Query: 664 TC-SRMFADPGQLNYPSFSVVFGSK-RVVRYTREVTNVGP-TGSVYEVATTSPSVVKVMV 723
            C +      G LNYPSFSVVF S   VV+Y R V NVG    +VYEV   SP+ V++ V
Sbjct: 661 ACETSKLRTAGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDV 720

Query: 724 KPTKLVFTKVGEKKRYTVTFTA---SSDAAQTTRFGFGSIAWSNDQHQFK 725
            P+KL F+K      Y VTF +              FGSI W++ +H  K
Sbjct: 721 SPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVK 762

BLAST of Sgr027318 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 9.7e-197
Identity = 367/748 (49.06%), Postives = 479/748 (64.04%), Query Frame = 0

Query: 21  VNAKRTYIVRMKHHALPSDYSTHHDWYSAHLQSLSSSITSDS------LLYTYTSAYHGF 80
           ++ K+TY++ M   A+P  Y+ H  WYS+ + S++   + +       +LYTY +A+HG 
Sbjct: 31  ISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNNRILYTYQTAFHGL 90

Query: 81  AASLDPEEAELLRQSDSVLGVYEDTVYDLHTTRTPGFLGLDSNFGLWEGHNTQDLDQASH 140
           AA L  EEAE L + D V+ V  +T Y+LHTTR+P FLGL+      E           H
Sbjct: 91  AAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQ----ESERVWAERVTDH 150

Query: 141 DVIIGVLDTGVWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQ 200
           DV++GVLDTG+WPES+SF+DTGM  +P+ WRG CE+G  F    CN+K++GAR F +GY+
Sbjct: 151 DVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGARVFYRGYE 210

Query: 201 MASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAA 260
            A+  G   +  E +SPRD+DGHGTHTA+T AGS V  A+L G+A G ARGMA +ARVAA
Sbjct: 211 AAT--GKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVAA 270

Query: 261 YKTCWPTGCFGSDILAA-----------------------------IGAFSAMEKGVFVS 320
           YK CW  GCF SDIL+A                             I  F AME GVFVS
Sbjct: 271 YKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGVFVS 330

Query: 321 CSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGM--GNK 380
           CSAGN GP+  SL NV+PWI TVGA T+DRDFPA V++G  + F GVSLY G+ +   NK
Sbjct: 331 CSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKNK 390

Query: 381 PVALVY---NKGSNTSSNMCLPGSLEPAIVRGKVVVCDRGINARVEKGGVVRDAGGIGMI 440
              LVY   N  S   ++ CL G+L+   V GK+V+CDRG+  RV+KG VV+ AGGIGM+
Sbjct: 391 QYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMV 450

Query: 441 LANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFGGTVLNVRPSPVV 500
           L NTA +GEELVADSH+LPAVAVG K G LI+QY  +  K TA L   GT + ++PSPVV
Sbjct: 451 LTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGIKPSPVV 510

Query: 501 AAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCP 560
           AAFSSRGPN ++ +ILKPD++ PGVNILAAW+  + P+ L SD R+ +FNI+SGTSMSCP
Sbjct: 511 AAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCP 570

Query: 561 HISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDP 620
           H+SG+AAL+K+ HP+WSP+AIKSALMTTAY  DN    L DA+G A S+P+ HGAGH+DP
Sbjct: 571 HVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGHIDP 630

Query: 621 HKALSPGLLYDLSTDDYIAFLCSLDYGIDHVQAIVKRPNVTCSRMFA-DPGQLNYPSFSV 680
            +A  PGL+YD+   +Y  FLC+ D     ++   K  N TC    A +PG LNYP+ S 
Sbjct: 631 LRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNYPAISA 690

Query: 681 VFGSK---RVVRYTREVTNVGPTGSVYEVATTSPSVVKVMVKPTKLVFTKVGEKKRYTVT 725
           +F      + +   R VTNVGP  S Y+V+ +      V V+P  L FT   +K  YTVT
Sbjct: 691 LFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQKLSYTVT 750

BLAST of Sgr027318 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 1.6e-191
Identity = 363/752 (48.27%), Postives = 485/752 (64.49%), Query Frame = 0

Query: 8   LIASLLLLLPCISV---NAKRTYIVRMKHHALPSDYSTHHDWYSAHLQSLSSSITSDSLL 67
           ++  L L  P IS     A +T+I R+   ++PS + TH+ WYS      S       ++
Sbjct: 6   IVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEES------RIV 65

Query: 68  YTYTSAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYDLHTTRTPGFLGLDSNFGLWEGH 127
           + Y + +HGF+A + P+EA+ LR   +VL V+ED   +LHTTR+P FLGL +  GLW   
Sbjct: 66  HVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLW--- 125

Query: 128 NTQDLDQASHDVIIGVLDTGVWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLI 187
              + D  S DVIIGV DTG+WPE +SF D  +  IP RWRG CESG  FSP  CN+K+I
Sbjct: 126 --SESDYGS-DVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKII 185

Query: 188 GARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLGYARGIAR 247
           GAR F+KG Q A  GG   K  E  SPRD DGHGTHT+STAAG H   AS+ GYA G+A+
Sbjct: 186 GARFFAKGQQAAVIGG-INKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAK 245

Query: 248 GMAPQARVAAYKTCW-PTGCFGSDILA--------------------------------A 307
           G+AP+AR+AAYK CW  +GC  SDILA                                A
Sbjct: 246 GVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIA 305

Query: 308 IGAFSAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTG 367
           IG++ A  KG+FVS SAGN GPN  S+ N+APW+ TVGA T+DR+FPA   LG+G R  G
Sbjct: 306 IGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRG 365

Query: 368 VSLYSGQGMGNKPVALVY-NKGSNTSSNMCLPGSLEPAIVRGKVVVCDRGINARVEKGGV 427
           VSLY+G  +  +   +VY  K   +S+++C+  +L+P  VRGK+V+CDRG + RV KG V
Sbjct: 366 VSLYAGVPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLV 425

Query: 428 VRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFGGT 487
           V+ AGG+GMILAN A++GE LV D+HL+PA AVG   GD I+ Y  S   P A + F GT
Sbjct: 426 VKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGT 485

Query: 488 VLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFN 547
           ++ ++P+PV+A+FS RGPN ++P+ILKPD+I PGVNILAAW++++GPTGL SD RKT+FN
Sbjct: 486 IVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFN 545

Query: 548 IMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNP 607
           I+SGTSM+CPH+SG AALLK+AHP+WSP+ I+SA+MTT    DN+N SL D + G  + P
Sbjct: 546 ILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATP 605

Query: 608 WAHGAGHVDPHKALSPGLLYDLSTDDYIAFLCSLDYGIDHVQAIVKRPNVTCSRMFADPG 667
           + +G+GH++  +A++PGL+YD++ DDYI FLCS+ YG   +Q I + P    +     PG
Sbjct: 606 YDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPG 665

Query: 668 QLNYPSFSVVFGSKR--VVRYT--REVTNVGPTGSVYEVATTSPSVVKVMVKPTKLVFTK 717
            LNYPS + VF + R  +V  T  R  TNVG   +VY     SP  V V VKP +LVFT 
Sbjct: 666 NLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTS 725

BLAST of Sgr027318 vs. ExPASy TrEMBL
Match: A0A6J1JIF3 (subtilisin-like protease SBT1.8 OS=Cucurbita maxima OX=3661 GN=LOC111484737 PE=3 SV=1)

HSP 1 Score: 1352.8 bits (3500), Expect = 0.0e+00
Identity = 683/756 (90.34%), Postives = 705/756 (93.25%), Query Frame = 0

Query: 1   MGSMARLLIAS---LLLLLPCISVNAKRTYIVRMKHHALPSDYSTHHDWYSAHLQSLSSS 60
           MGSMARLL+AS   LLLLLPC+ VNAKRTYIVRMKHHALPSDY THHDWYSAHLQSLSSS
Sbjct: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60

Query: 61  ITSDSLLYTYTSAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYDLHTTRTPGFLGLDSN 120
            TSDSLLYTYTSAYHGFAASLDP+EAELLRQSDSVLGVYEDTVY LHTTRTPGFLGLDS+
Sbjct: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYKLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGVWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180
           FGLWEGHNTQDLDQASHDVIIGVLDTG+WPESKSFDDTGMPEIPSRWRGECESGPDFSPS
Sbjct: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYFRK RENESPRDQDGHGTHTASTAAGSHVANASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILA--------------------------- 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILA                           
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 --AIGAFSAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
             AIGAF+AMEKGVFVSCSAGNSGPN+ASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 FTGVSLYSGQGMGNKPVALVYNKGSNTSSNMCLPGSLEPAIVRGKVVVCDRGINARVEKG 420
           FTGVSLYSGQGMGNKPVALVYNKGSN+SSNMCLPGSLEPA VRGKVV+CDRGINARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYVRSV+KPTAVLSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480

Query: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQ 540
           GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQ
Sbjct: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
           FNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGMFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLLYDLSTDDYIAFLCSLDYGIDHVQAIVKRPNVTCSRMFAD 660
           NPWAHGAGHVDPHKALSPGLLYD+ST+DYI FLCSLDYGIDHVQAIVKR N+TCS+ FAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLLYDISTNDYITFLCSLDYGIDHVQAIVKRSNITCSKKFAD 660

Query: 661 PGQLNYPSFSVVFGSKRVVRYTREVTNVGPTGSVYEVATTSPSVVKVMVKPTKLVFTKVG 720
           PGQLNYPSFSVVFGSKRVVRYTR VTNVG  GSVYEVATT+PSVVKV VKP+KLVF+KVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720

Query: 721 EKKRYTVTFTASSDAAQTTRFGFGSIAWSNDQHQFK 725
           E+KRYTVTF AS DAA+TTR+GFGSIAWSNDQHQ +
Sbjct: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVR 756

BLAST of Sgr027318 vs. ExPASy TrEMBL
Match: A0A6J1H8F1 (subtilisin-like protease SBT1.8 OS=Cucurbita moschata OX=3662 GN=LOC111461488 PE=3 SV=1)

HSP 1 Score: 1350.5 bits (3494), Expect = 0.0e+00
Identity = 681/756 (90.08%), Postives = 705/756 (93.25%), Query Frame = 0

Query: 1   MGSMARLLIAS---LLLLLPCISVNAKRTYIVRMKHHALPSDYSTHHDWYSAHLQSLSSS 60
           MGSMARLL+AS   LLLLLPC+ VNAKRTYIVRMKHHALPSDY THHDWYSAHLQSLSSS
Sbjct: 1   MGSMARLLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSLSSS 60

Query: 61  ITSDSLLYTYTSAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYDLHTTRTPGFLGLDSN 120
            TSDSLLYTYTSAYHGFAASLDP+EAELLRQSDSVLGVYEDTVY+LHTTRTPGFLGLDS+
Sbjct: 61  ATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 120

Query: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGVWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180
           FGLWEGHNTQDLDQASHDVIIGVLDTG+WPESKSFDDTGMPEIPSRWRGECESGPDFSPS
Sbjct: 121 FGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLG 240
           LCNKKLIGARSFSKGYQMASGGGYFRK RENESPRDQDGHGTHTASTAAGSHVANASLLG
Sbjct: 181 LCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANASLLG 240

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILA--------------------------- 300
           YARGIARGMAPQARVAAYKTCWPTGCFGSDILA                           
Sbjct: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRD 300

Query: 301 --AIGAFSAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
             AIGAF+AMEKGVFVSCSAGNSGPN+ASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR
Sbjct: 301 TIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360

Query: 361 FTGVSLYSGQGMGNKPVALVYNKGSNTSSNMCLPGSLEPAIVRGKVVVCDRGINARVEKG 420
           FTGVSLYSGQGMGNKPVALVYNKGSN+SSNMCLPGSLEPA VRGKVV+CDRGINARVEKG
Sbjct: 361 FTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARVEKG 420

Query: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFG 480
           GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYVRSV+KPTAVLSFG
Sbjct: 421 GVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVLSFG 480

Query: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQ 540
           GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD+DKRKTQ
Sbjct: 481 GTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQ 540

Query: 541 FNIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600
           FNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS
Sbjct: 541 FNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFS 600

Query: 601 NPWAHGAGHVDPHKALSPGLLYDLSTDDYIAFLCSLDYGIDHVQAIVKRPNVTCSRMFAD 660
           NPWAHGAGHVDPHKALSPGL+YD+ST+DYI FLCSLDYGIDHVQAI KR N+TC + FAD
Sbjct: 601 NPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKKFAD 660

Query: 661 PGQLNYPSFSVVFGSKRVVRYTREVTNVGPTGSVYEVATTSPSVVKVMVKPTKLVFTKVG 720
           PGQLNYPSFSVVFGSKRVVRYTR VTNVG  GSVYEVATT+PSVVKV VKP+KLVF+KVG
Sbjct: 661 PGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFSKVG 720

Query: 721 EKKRYTVTFTASSDAAQTTRFGFGSIAWSNDQHQFK 725
           E+KRYTVTF AS DAA+TTR+GFGSIAWSNDQHQ +
Sbjct: 721 ERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVR 756

BLAST of Sgr027318 vs. ExPASy TrEMBL
Match: A0A5D3CDV9 (Subtilisin-like protease SBT1.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001530 PE=3 SV=1)

HSP 1 Score: 1315.1 bits (3402), Expect = 0.0e+00
Identity = 664/754 (88.06%), Postives = 693/754 (91.91%), Query Frame = 0

Query: 1   MGSMARLLIA-SLLLLLPCISVNAKRTYIVRMKHHALPSDYSTHHDWYSAHLQSLSSSIT 60
           M SM R LI   LLLLL C+ +NAK+TYIV MKHHALPS+Y THHDWYSA LQSLSSS +
Sbjct: 1   MDSMPRFLIPFLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSSS 60

Query: 61  SDSLLYTYTSAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYDLHTTRTPGFLGLDSNFG 120
           SDSLLYTYTS++HGFAA LD EE ELLRQSDSVLGVYEDTVY+LHTTRTPGFLGLDS+FG
Sbjct: 61  SDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFG 120

Query: 121 LWEGHNTQDLDQASHDVIIGVLDTGVWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLC 180
           LWEGH TQDL+QASHDVIIGVLDTG+WPESKSFDDTGMPEIPSRWRGECE+GPDFSPSLC
Sbjct: 121 LWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECEAGPDFSPSLC 180

Query: 181 NKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLGYA 240
           NKKLIGARSFSKGYQMASGGGYFRKPREN+S RDQDGHGTHTASTAAGSHV NASLLGYA
Sbjct: 181 NKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGYA 240

Query: 241 RGIARGMAPQARVAAYKTCWPTGCFGSDILA----------------------------- 300
           RGIARGMAPQARVAAYKTCWPTGCFGSDILA                             
Sbjct: 241 RGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTI 300

Query: 301 AIGAFSAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFT 360
           AIGAF+AMEKGVFVSCSAGNSGPN+ASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFT
Sbjct: 301 AIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFT 360

Query: 361 GVSLYSGQGMGNKPVALVYNKGSNTSSNMCLPGSLEPAIVRGKVVVCDRGINARVEKGGV 420
           GVSLYSGQGMGNK VALVYNKGSNTSSNMCLPGSL+PA+VRGKVVVCDRGINARVEKGGV
Sbjct: 361 GVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGGV 420

Query: 421 VRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFGGT 480
           VRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRS S PTAVLSFGGT
Sbjct: 421 VRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGGT 480

Query: 481 VLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFN 540
           +LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGL++DKRKTQFN
Sbjct: 481 ILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQFN 540

Query: 541 IMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNP 600
           IMSGTSMSCPHISGLAALLKAAHP+WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FSNP
Sbjct: 541 IMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNP 600

Query: 601 WAHGAGHVDPHKALSPGLLYDLSTDDYIAFLCSLDYGIDHVQAIVKRPNVTCSRMFADPG 660
           WAHGAGHVDPHKALSPGLLYD+ST+DYIAFLCSLDYGIDHVQAIVKR N+TCSR FADPG
Sbjct: 601 WAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADPG 660

Query: 661 QLNYPSFSVVFGSKRVVRYTREVTNVGPTGSVYEVATTSPSVVKVMVKPTKLVFTKVGEK 720
           QLNYPSFSVVFGSKRVVRYTR VTNVG  GSVY+VATT+PSVVKV VKP+KLVFTKVGE+
Sbjct: 661 QLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGER 720

Query: 721 KRYTVTFTASSDAAQTTRFGFGSIAWSNDQHQFK 725
           KRYTVTF AS DAAQTTRFGFGSI WSNDQHQ +
Sbjct: 721 KRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVR 754

BLAST of Sgr027318 vs. ExPASy TrEMBL
Match: A0A5A7UKV3 (Subtilisin-like protease SBT1.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001560 PE=3 SV=1)

HSP 1 Score: 1312.0 bits (3394), Expect = 0.0e+00
Identity = 663/755 (87.81%), Postives = 692/755 (91.66%), Query Frame = 0

Query: 1   MGSMARLLI--ASLLLLLPCISVNAKRTYIVRMKHHALPSDYSTHHDWYSAHLQSLSSSI 60
           M SM R LI    LLLLL C+ +NAK+TYIV MKHHALPS+Y THHDWYSA LQSLSSS 
Sbjct: 1   MDSMPRFLIPFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSS 60

Query: 61  TSDSLLYTYTSAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYDLHTTRTPGFLGLDSNF 120
           +SDSLLYTYTS++HGFAA LD EE ELLRQSDSVLGVYEDTVY+LHTTRTPGFLGLDS+F
Sbjct: 61  SSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120

Query: 121 GLWEGHNTQDLDQASHDVIIGVLDTGVWPESKSFDDTGMPEIPSRWRGECESGPDFSPSL 180
           GLWEGH TQDL+QASHDVIIGVLDTG+WPESKSFDDTGMPEIPSRWRG CE+GPDFSPSL
Sbjct: 121 GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSL 180

Query: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLGY 240
           CNKKLIGARSFSKGYQMASGGGYFRKPREN+S RDQDGHGTHTASTAAGSHV NASLLGY
Sbjct: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGY 240

Query: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILA---------------------------- 300
           ARGIARGMAPQARVAAYKTCWPTGCFGSDILA                            
Sbjct: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300

Query: 301 -AIGAFSAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
            AIGAF+AMEKGVFVSCSAGNSGPN+ASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF
Sbjct: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360

Query: 361 TGVSLYSGQGMGNKPVALVYNKGSNTSSNMCLPGSLEPAIVRGKVVVCDRGINARVEKGG 420
           TGVSLYSGQGMGNK VALVYNKGSNTSSNMCLPGSL+PA+VRGKVVVCDRGINARVEKGG
Sbjct: 361 TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGG 420

Query: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFGG 480
           VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRS S PTAVLSFGG
Sbjct: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG 480

Query: 481 TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQF 540
           T+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGL++DKRKTQF
Sbjct: 481 TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF 540

Query: 541 NIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSN 600
           NIMSGTSMSCPHISGLAALLKAAHP+WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FSN
Sbjct: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN 600

Query: 601 PWAHGAGHVDPHKALSPGLLYDLSTDDYIAFLCSLDYGIDHVQAIVKRPNVTCSRMFADP 660
           PWAHGAGHVDPHKALSPGLLYD+ST+DYIAFLCSLDYGIDHVQAIVKR N+TCSR FADP
Sbjct: 601 PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP 660

Query: 661 GQLNYPSFSVVFGSKRVVRYTREVTNVGPTGSVYEVATTSPSVVKVMVKPTKLVFTKVGE 720
           GQLNYPSFSVVFGSKRVVRYTR VTNVG  GSVY+VATT+PSVVKV VKP+KLVFTKVGE
Sbjct: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGE 720

Query: 721 KKRYTVTFTASSDAAQTTRFGFGSIAWSNDQHQFK 725
           +KRYTVTF AS DAAQTTRFGFGSI WSNDQHQ +
Sbjct: 721 RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVR 755

BLAST of Sgr027318 vs. ExPASy TrEMBL
Match: A0A1S3BQE8 (subtilisin-like protease SBT1.8 OS=Cucumis melo OX=3656 GN=LOC103492374 PE=3 SV=1)

HSP 1 Score: 1312.0 bits (3394), Expect = 0.0e+00
Identity = 663/755 (87.81%), Postives = 692/755 (91.66%), Query Frame = 0

Query: 1   MGSMARLLI--ASLLLLLPCISVNAKRTYIVRMKHHALPSDYSTHHDWYSAHLQSLSSSI 60
           M SM R LI    LLLLL C+ +NAK+TYIV MKHHALPS+Y THHDWYSA LQSLSSS 
Sbjct: 1   MDSMPRFLIPFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSS 60

Query: 61  TSDSLLYTYTSAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYDLHTTRTPGFLGLDSNF 120
           +SDSLLYTYTS++HGFAA LD EE ELLRQSDSVLGVYEDTVY+LHTTRTPGFLGLDS+F
Sbjct: 61  SSDSLLYTYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDF 120

Query: 121 GLWEGHNTQDLDQASHDVIIGVLDTGVWPESKSFDDTGMPEIPSRWRGECESGPDFSPSL 180
           GLWEGH TQDL+QASHDVIIGVLDTG+WPESKSFDDTGMPEIPSRWRG CE+GPDFSPSL
Sbjct: 121 GLWEGHTTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSL 180

Query: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLGY 240
           CNKKLIGARSFSKGYQMASGGGYFRKPREN+S RDQDGHGTHTASTAAGSHV NASLLGY
Sbjct: 181 CNKKLIGARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGY 240

Query: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILA---------------------------- 300
           ARGIARGMAPQARVAAYKTCWPTGCFGSDILA                            
Sbjct: 241 ARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDT 300

Query: 301 -AIGAFSAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360
            AIGAF+AMEKGVFVSCSAGNSGPN+ASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF
Sbjct: 301 IAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRF 360

Query: 361 TGVSLYSGQGMGNKPVALVYNKGSNTSSNMCLPGSLEPAIVRGKVVVCDRGINARVEKGG 420
           TGVSLYSGQGMGNK VALVYNKGSNTSSNMCLPGSL+PA+VRGKVVVCDRGINARVEKGG
Sbjct: 361 TGVSLYSGQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGG 420

Query: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFGG 480
           VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRS S PTAVLSFGG
Sbjct: 421 VVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGG 480

Query: 481 TVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQF 540
           T+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGL++DKRKTQF
Sbjct: 481 TILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQF 540

Query: 541 NIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSN 600
           NIMSGTSMSCPHISGLAALLKAAHP+WSPSAIKSALMTTAYTQDNTNSSLRDAAGG FSN
Sbjct: 541 NIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSN 600

Query: 601 PWAHGAGHVDPHKALSPGLLYDLSTDDYIAFLCSLDYGIDHVQAIVKRPNVTCSRMFADP 660
           PWAHGAGHVDPHKALSPGLLYD+ST+DYIAFLCSLDYGIDHVQAIVKR N+TCSR FADP
Sbjct: 601 PWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADP 660

Query: 661 GQLNYPSFSVVFGSKRVVRYTREVTNVGPTGSVYEVATTSPSVVKVMVKPTKLVFTKVGE 720
           GQLNYPSFSVVFGSKRVVRYTR VTNVG  GSVY+VATT+PSVVKV VKP+KLVFTKVGE
Sbjct: 661 GQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGE 720

Query: 721 KKRYTVTFTASSDAAQTTRFGFGSIAWSNDQHQFK 725
           +KRYTVTF AS DAAQTTRFGFGSI WSNDQHQ +
Sbjct: 721 RKRYTVTFVASRDAAQTTRFGFGSIVWSNDQHQVR 755

BLAST of Sgr027318 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 1014.2 bits (2621), Expect = 9.4e-296
Identity = 515/747 (68.94%), Postives = 596/747 (79.79%), Query Frame = 0

Query: 8   LIASLLLLLPCISVNAKRTYIVRMKHHALPSDYSTHHDWYSAHLQSLSSSITSDSLLYTY 67
           +I + L LL  +   AK+TYI+R+ H   P  + THHDWY++ L S S      SLLYTY
Sbjct: 13  IITTFLFLL--LHTTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNSES------SLLYTY 72

Query: 68  TSAYHGFAASLDPEEAE-LLRQSDSVLGVYEDTVYDLHTTRTPGFLGLDSNFGLWEGHNT 127
           T+++HGF+A LD  EA+ LL  S+S+L ++ED +Y LHTTRTP FLGL+S FG+      
Sbjct: 73  TTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGV------ 132

Query: 128 QDLDQASHDVIIGVLDTGVWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGA 187
            DL  +S+ VIIGVLDTGVWPES+SFDDT MPEIPS+W+GECESG DF   LCNKKLIGA
Sbjct: 133 HDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGA 192

Query: 188 RSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGM 247
           RSFSKG+QMASGGG+  K RE+ SPRD DGHGTHT++TAAGS V NAS LGYA G ARGM
Sbjct: 193 RSFSKGFQMASGGGFSSK-RESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGM 252

Query: 248 APQARVAAYKTCWPTGCFGSDILA-----------------------------AIGAFSA 307
           A +ARVA YK CW TGCFGSDILA                             AIGAFSA
Sbjct: 253 ATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSA 312

Query: 308 MEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSG 367
           ME+GVFVSCSAGNSGP RAS+ANVAPW+MTVGAGTLDRDFPA+  LGNGKR TGVSLYSG
Sbjct: 313 MERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSG 372

Query: 368 QGMGNKPVALVYNKGSNTSSNMCLPGSLEPAIVRGKVVVCDRGINARVEKGGVVRDAGGI 427
            GMG KP+ LVYNKG+++SSN+CLPGSL+ +IVRGK+VVCDRG+NARVEKG VVRDAGG+
Sbjct: 373 VGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGL 432

Query: 428 GMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFGGTVLNVRPS 487
           GMI+ANTAASGEELVADSHLLPA+AVG+KTGDL+R+YV+S SKPTA+L F GTVL+V+PS
Sbjct: 433 GMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPS 492

Query: 488 PVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSM 547
           PVVAAFSSRGPN VTP+ILKPDVIGPGVNILA WS++IGPTGLD D R+TQFNIMSGTSM
Sbjct: 493 PVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSM 552

Query: 548 SCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGH 607
           SCPHISGLA LLKAAHPEWSPSAIKSALMTTAY  DNTN+ L DAA  + SNP+AHG+GH
Sbjct: 553 SCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGH 612

Query: 608 VDPHKALSPGLLYDLSTDDYIAFLCSLDYGIDHVQAIVKRPNVTCSRMFADPGQLNYPSF 667
           VDP KALSPGL+YD+ST++YI FLCSLDY +DH+ AIVKRP+V CS+ F+DPGQLNYPSF
Sbjct: 613 VDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSF 672

Query: 668 SVVFGSKRVVRYTREVTNVGPTGSVYEVATTSPSVVKVMVKPTKLVFTKVGEKKRYTVTF 725
           SV+FG KRVVRYTREVTNVG   SVY+V       V + VKP+KL F  VGEKKRYTVTF
Sbjct: 673 SVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTF 732

BLAST of Sgr027318 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 742.7 bits (1916), Expect = 5.3e-214
Identity = 407/757 (53.76%), Postives = 512/757 (67.64%), Query Frame = 0

Query: 1   MGSMARLLIASLLLLLPCISVNAKRTYIVRMKHHALPSDYSTHHDWYSAHLQSLSSSITS 60
           + S A  L+  L       S + + TYIV M    +PS +  H +WY + L+S+S S   
Sbjct: 6   LSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSA-- 65

Query: 61  DSLLYTYTSAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYDLHTTRTPGFLGLDSNFGL 120
             LLYTY +A HGF+  L  EEA+ L     V+ V  +  Y+LHTTRTP FLGLD     
Sbjct: 66  -ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLD----- 125

Query: 121 WEGHNTQDL--DQASH-DVIIGVLDTGVWPESKSFDDTGMPEIPSRWRGECESGPDFSPS 180
               +T DL  +  S+ DV++GVLDTGVWPESKS+ D G   IPS W+G CE+G +F+ S
Sbjct: 126 ---EHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTAS 185

Query: 181 LCNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLG 240
           LCN+KLIGAR F++GY+  S  G   + +E+ SPRD DGHGTHT+STAAGS V  ASLLG
Sbjct: 186 LCNRKLIGARFFARGYE--STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLG 245

Query: 241 YARGIARGMAPQARVAAYKTCWPTGCFGSDILA--------------------------- 300
           YA G ARGMAP+ARVA YK CW  GCF SDILA                           
Sbjct: 246 YASGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRD 305

Query: 301 --AIGAFSAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKR 360
             AIGAF+AME+G+ VSCSAGN+GP+ +SL+NVAPWI TVGAGTLDRDFPA   LGNGK 
Sbjct: 306 GVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKN 365

Query: 361 FTGVSLYSGQGMGNKPVALVY--NKGSNTSSNMCLPGSLEPAIVRGKVVVCDRGINARVE 420
           FTGVSL+ G+ + +K +  +Y  N  + T+ N+C+ G+L P  V+GK+V+CDRGINARV+
Sbjct: 366 FTGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQ 425

Query: 421 KGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLS 480
           KG VV+ AGG+GMILANTAA+GEELVAD+HLLPA  VG K GD+IR YV +   PTA +S
Sbjct: 426 KGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASIS 485

Query: 481 FGGTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRK 540
             GTV+ V+PSPVVAAFSSRGPN +TP ILKPD+I PGVNILAAW+ + GPTGL SD R+
Sbjct: 486 ILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRR 545

Query: 541 TQFNIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGA 600
            +FNI+SGTSMSCPH+SGLAALLK+ HPEWSP+AI+SALMTTAY        L D A G 
Sbjct: 546 VEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGK 605

Query: 601 FSNPWAHGAGHVDPHKALSPGLLYDLSTDDYIAFLCSLDYGIDHVQAIVKRPNVTC--SR 660
            S P+ HGAGHV P  A +PGL+YDL+T+DY+ FLC+L+Y    ++++ +R N TC  S+
Sbjct: 606 PSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRR-NYTCDPSK 665

Query: 661 MFADPGQLNYPSFSVVFGSKRVVRYTREVTNVGPTGSVYEVATTSPSVVKVMVKPTKLVF 720
            ++    LNYPSF+V        +YTR VT+VG  G+     T+  + VK+ V+P  L F
Sbjct: 666 SYS-VADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNF 725

Query: 721 TKVGEKKRYTVTFTASSDAAQTTRFGFGSIAWSNDQH 722
            +  EKK YTVTFT  S     +   FGSI WS+ +H
Sbjct: 726 KEANEKKSYTVTFTVDSSKPSGSN-SFGSIEWSDGKH 746

BLAST of Sgr027318 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 709.1 bits (1829), Expect = 6.4e-204
Identity = 388/770 (50.39%), Postives = 494/770 (64.16%), Query Frame = 0

Query: 4   MARLLIASLLLLLPCI---------SVNAKRTYIVRMKHHALPSDYSTHHDWYSAHLQSL 63
           MA+L ++S+  + P +         S +   +YIV ++    PS +S+H++W+ + L+SL
Sbjct: 1   MAKLSLSSIFFVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSL 60

Query: 64  SSSITSDSLLYTYTSAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYDLHTTRTPGFLGL 123
            SS    +LLY+Y+ A HGF+A L P +   LR+  SV+ V  D   ++HTT TP FLG 
Sbjct: 61  PSSPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGF 120

Query: 124 DSNFGLWEGHNTQDLDQASHDVIIGVLDTGVWPESKSFDDTGMPEIPSRWRGECESGPDF 183
             N GLW   N         DVI+GVLDTG+WPE  SF D+G+  IPS W+GECE GPDF
Sbjct: 121 SQNSGLWSNSN------YGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDF 180

Query: 184 SPSLCNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANAS 243
             S CN+KLIGAR+F +GY     G      +E+ SPRD +GHGTHTASTAAGS VANAS
Sbjct: 181 PASSCNRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANAS 240

Query: 244 LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILA------------------------ 303
           L  YARG A GMA +AR+AAYK CW  GC+ SDILA                        
Sbjct: 241 LYQYARGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAP 300

Query: 304 -------AIGAFSAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQL 363
                  AIGAF A   G+ VSCSAGNSGPN  +  N+APWI+TVGA T+DR+F A    
Sbjct: 301 EYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAIT 360

Query: 364 GNGKRFTGVSLYSGQGMGNKPVALVYNKGSNTSSNMCLPGSLEPAIVRGKVVVCDRGINA 423
           G+GK FTG SLY+G+ + +  ++LVY+   +  S +C PG L  ++V GK+V+CDRG NA
Sbjct: 361 GDGKVFTGTSLYAGESLPDSQLSLVYS--GDCGSRLCYPGKLNSSLVEGKIVLCDRGGNA 420

Query: 424 RVEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTA 483
           RVEKG  V+ AGG GMILANTA SGEEL ADSHL+PA  VG K GD IR Y+++   PTA
Sbjct: 421 RVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTA 480

Query: 484 VLSFGGTVLNVR-PSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDS 543
            +SF GT++    PSP VAAFSSRGPN +TP ILKPDVI PGVNILA W+  +GPT LD 
Sbjct: 481 KISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDI 540

Query: 544 DKRKTQFNIMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDA 603
           D R+ QFNI+SGTSMSCPH+SGLAALL+ AHP+WSP+AIKSAL+TTAY  +N+   + D 
Sbjct: 541 DPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDL 600

Query: 604 AGGAFSNPWAHGAGHVDPHKALSPGLLYDLSTDDYIAFLCSLDYGIDHVQAIVKRPNV-- 663
           A G  SN + HGAGHVDP+KAL+PGL+YD+   +Y+AFLC++ Y    +   ++ P +  
Sbjct: 601 ATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYD 660

Query: 664 TC-SRMFADPGQLNYPSFSVVFGSK-RVVRYTREVTNVGP-TGSVYEVATTSPSVVKVMV 723
            C +      G LNYPSFSVVF S   VV+Y R V NVG    +VYEV   SP+ V++ V
Sbjct: 661 ACETSKLRTAGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDV 720

Query: 724 KPTKLVFTKVGEKKRYTVTFTA---SSDAAQTTRFGFGSIAWSNDQHQFK 725
            P+KL F+K      Y VTF +              FGSI W++ +H  K
Sbjct: 721 SPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVK 762

BLAST of Sgr027318 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 689.1 bits (1777), Expect = 6.9e-198
Identity = 367/748 (49.06%), Postives = 479/748 (64.04%), Query Frame = 0

Query: 21  VNAKRTYIVRMKHHALPSDYSTHHDWYSAHLQSLSSSITSDS------LLYTYTSAYHGF 80
           ++ K+TY++ M   A+P  Y+ H  WYS+ + S++   + +       +LYTY +A+HG 
Sbjct: 31  ISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNNRILYTYQTAFHGL 90

Query: 81  AASLDPEEAELLRQSDSVLGVYEDTVYDLHTTRTPGFLGLDSNFGLWEGHNTQDLDQASH 140
           AA L  EEAE L + D V+ V  +T Y+LHTTR+P FLGL+      E           H
Sbjct: 91  AAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQ----ESERVWAERVTDH 150

Query: 141 DVIIGVLDTGVWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLIGARSFSKGYQ 200
           DV++GVLDTG+WPES+SF+DTGM  +P+ WRG CE+G  F    CN+K++GAR F +GY+
Sbjct: 151 DVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGARVFYRGYE 210

Query: 201 MASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAA 260
            A+  G   +  E +SPRD+DGHGTHTA+T AGS V  A+L G+A G ARGMA +ARVAA
Sbjct: 211 AAT--GKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVAA 270

Query: 261 YKTCWPTGCFGSDILAA-----------------------------IGAFSAMEKGVFVS 320
           YK CW  GCF SDIL+A                             I  F AME GVFVS
Sbjct: 271 YKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGVFVS 330

Query: 321 CSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGM--GNK 380
           CSAGN GP+  SL NV+PWI TVGA T+DRDFPA V++G  + F GVSLY G+ +   NK
Sbjct: 331 CSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKNK 390

Query: 381 PVALVY---NKGSNTSSNMCLPGSLEPAIVRGKVVVCDRGINARVEKGGVVRDAGGIGMI 440
              LVY   N  S   ++ CL G+L+   V GK+V+CDRG+  RV+KG VV+ AGGIGM+
Sbjct: 391 QYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMV 450

Query: 441 LANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFGGTVLNVRPSPVV 500
           L NTA +GEELVADSH+LPAVAVG K G LI+QY  +  K TA L   GT + ++PSPVV
Sbjct: 451 LTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGIKPSPVV 510

Query: 501 AAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFNIMSGTSMSCP 560
           AAFSSRGPN ++ +ILKPD++ PGVNILAAW+  + P+ L SD R+ +FNI+SGTSMSCP
Sbjct: 511 AAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCP 570

Query: 561 HISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNPWAHGAGHVDP 620
           H+SG+AAL+K+ HP+WSP+AIKSALMTTAY  DN    L DA+G A S+P+ HGAGH+DP
Sbjct: 571 HVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGHIDP 630

Query: 621 HKALSPGLLYDLSTDDYIAFLCSLDYGIDHVQAIVKRPNVTCSRMFA-DPGQLNYPSFSV 680
            +A  PGL+YD+   +Y  FLC+ D     ++   K  N TC    A +PG LNYP+ S 
Sbjct: 631 LRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNYPAISA 690

Query: 681 VFGSK---RVVRYTREVTNVGPTGSVYEVATTSPSVVKVMVKPTKLVFTKVGEKKRYTVT 725
           +F      + +   R VTNVGP  S Y+V+ +      V V+P  L FT   +K  YTVT
Sbjct: 691 LFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQKLSYTVT 750

BLAST of Sgr027318 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 671.8 bits (1732), Expect = 1.1e-192
Identity = 363/752 (48.27%), Postives = 485/752 (64.49%), Query Frame = 0

Query: 8   LIASLLLLLPCISV---NAKRTYIVRMKHHALPSDYSTHHDWYSAHLQSLSSSITSDSLL 67
           ++  L L  P IS     A +T+I R+   ++PS + TH+ WYS      S       ++
Sbjct: 6   IVLLLFLSFPFISFAASQAAKTFIFRIDGGSMPSIFPTHYHWYSTEFAEES------RIV 65

Query: 68  YTYTSAYHGFAASLDPEEAELLRQSDSVLGVYEDTVYDLHTTRTPGFLGLDSNFGLWEGH 127
           + Y + +HGF+A + P+EA+ LR   +VL V+ED   +LHTTR+P FLGL +  GLW   
Sbjct: 66  HVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLW--- 125

Query: 128 NTQDLDQASHDVIIGVLDTGVWPESKSFDDTGMPEIPSRWRGECESGPDFSPSLCNKKLI 187
              + D  S DVIIGV DTG+WPE +SF D  +  IP RWRG CESG  FSP  CN+K+I
Sbjct: 126 --SESDYGS-DVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKII 185

Query: 188 GARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLGYARGIAR 247
           GAR F+KG Q A  GG   K  E  SPRD DGHGTHT+STAAG H   AS+ GYA G+A+
Sbjct: 186 GARFFAKGQQAAVIGG-INKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAK 245

Query: 248 GMAPQARVAAYKTCW-PTGCFGSDILA--------------------------------A 307
           G+AP+AR+AAYK CW  +GC  SDILA                                A
Sbjct: 246 GVAPKARIAAYKVCWKDSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIA 305

Query: 308 IGAFSAMEKGVFVSCSAGNSGPNRASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTG 367
           IG++ A  KG+FVS SAGN GPN  S+ N+APW+ TVGA T+DR+FPA   LG+G R  G
Sbjct: 306 IGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRG 365

Query: 368 VSLYSGQGMGNKPVALVY-NKGSNTSSNMCLPGSLEPAIVRGKVVVCDRGINARVEKGGV 427
           VSLY+G  +  +   +VY  K   +S+++C+  +L+P  VRGK+V+CDRG + RV KG V
Sbjct: 366 VSLYAGVPLNGRMFPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLV 425

Query: 428 VRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSVSKPTAVLSFGGT 487
           V+ AGG+GMILAN A++GE LV D+HL+PA AVG   GD I+ Y  S   P A + F GT
Sbjct: 426 VKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGT 485

Query: 488 VLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDSDKRKTQFN 547
           ++ ++P+PV+A+FS RGPN ++P+ILKPD+I PGVNILAAW++++GPTGL SD RKT+FN
Sbjct: 486 IVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFN 545

Query: 548 IMSGTSMSCPHISGLAALLKAAHPEWSPSAIKSALMTTAYTQDNTNSSLRDAAGGAFSNP 607
           I+SGTSM+CPH+SG AALLK+AHP+WSP+ I+SA+MTT    DN+N SL D + G  + P
Sbjct: 546 ILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATP 605

Query: 608 WAHGAGHVDPHKALSPGLLYDLSTDDYIAFLCSLDYGIDHVQAIVKRPNVTCSRMFADPG 667
           + +G+GH++  +A++PGL+YD++ DDYI FLCS+ YG   +Q I + P    +     PG
Sbjct: 606 YDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPG 665

Query: 668 QLNYPSFSVVFGSKR--VVRYT--REVTNVGPTGSVYEVATTSPSVVKVMVKPTKLVFTK 717
            LNYPS + VF + R  +V  T  R  TNVG   +VY     SP  V V VKP +LVFT 
Sbjct: 666 NLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTS 725