Sgr027089 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr027089
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionH(+)-exporting diphosphatase
Locationtig00153048: 929406 .. 933942 (-)
RNA-Seq ExpressionSgr027089
SyntenySgr027089
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCGTCACCATTCTTCCAGATCTCGGGACTCAGATTCTGATTCCGGTTTGTGCCATAGTCGGAATCGTCTTCTCCTTGGTGCAGTGGTACTATGTTTCCCAAGTTAAGCTCTCGCCTGGTCGGGATTCCGCCCATAACAACAGCAGCGCCGGTAAGAACGGCTACTCCGACTACCTTATAGAGGAAGAAGAGGGCGTCAACGACCACAGCGTTGTCATCAAGTGTGCCGAAATTCAAAACGCCATCTCCGAGGGTAAACGCTTTCTCTTCTTCTTCTTCTTCTCTTCGCTTCAGATTTGCTTCCTCACTAACCTTCTTGGGTTTCTGCTCGTTTTTTTTTCCTTATTTATTGCTAGATCTGGTCTTGGATTCTGAGATTTATGGTTCTTAGATTTTATTTTTCTCTTTCATGGAATTTTCGCTTATTTTCCTTTCGTGAACGCTTTAGGTTTTACCTCTTTTTTTCTCTCTCCTGTATTGTCATTTGTCACTTCTTGCTTTGCTATTTGTTTTCGGGGTCTTCGTTGCTGGAGCAATTTCTTTGTTTTTTTTTTTTTTTTTTGTTAAAGTACGAACTTCAGATTCTTATTTGGAAGGTTTCATATTGTGTCATTTTTACAAGATTGTTTTTTCTACTCTAATATCGTGTGTAGTAGACGTGTGGATATTACATTGGTCCTATCATTCCATGCATCTCCAGGCCAAGCTTGTTATTTTAGGCTGTTGCGTTCTGGACCCTGTGTGGCATGGGAATCCATCCCTGCTTGACGTCAAACCAGCATGAGAACTGGGTCACTGGTCATACCGCATTTTCTTAAATAGGTTACGGAACCATTAATTTCCAATTAATTATTTAATGTGATTGGTTATGGTGTCTGTCCACATGTAGTTTATTGGTTTAGCAAATTTAGGGGCAGGGGTCAAGGTCGGCCAGTGAGACGATGGGGAGGATTATGACTTTTTATGTCTGAAGGATTCTTTCAAGTTCTATCCCCGCATCGTGTAATCTAGCATCTACAAAGTAATCATGAAAACGGGAATTTACGAAGAGATCTTGAAAATGATGATTGAAATAATTTCAGTTTATTAAATAACAGAGGAAGACGCATTATCAGATTTCTTGGAACTTATTCTCTCTCGGTAAAGTTGTTATCCATTTGGGTTTGTAGTGTATCTATTGGTCATAGGAAGAGGTCCATCACCACCTCTAGAGCTAATCAGCATTTTTCAAAGTTTATGAGATTCTTGTGTCTTTGCATCGGGTTCTATGTTATAGTGTCTTGTTTTGTTCTTGTGATATTTATGTTATTGTTGTTTTCATATATACTGATGCAAAGAATAATATCAATATAAATCTTTGGTAGCATTTTTATGAGTTTCTTCGTCTGTGCCTTCTGGAATCATTTTTTCCATGTGATATTTATGCATTCCTTTTATCGAAGATGTCATCTTCCAGAAACTAAAATTCTTATGTCAAGATTTTTTTGCTTCTTGCGACGGAAAAAAGTAAAAAGTATTATTGTATCTATTCAGAAAAATTGTAGGAATATGAATAGTGTTAGATGGTAAAACCAGTTGCTTTCCCTAAATGATAAAATGTTTGTTCTTTAAAAACTTATAGTTGTTTACTCTCACCTTGAATAGTTGATTATTTCCATTTTGTTCTAGTTTTCATTTTTGCCTTTTTATATTAATTTATTCATAAAATGAAGCCTTGTGGAATAATTTCTTTTCTGACAGAATTACTTTGGAGCTGTTTCTAACCGTTTTAACTCTTTGATGTATTAGGAGCAACCTCCTTCCTTTTCACGGAGTATAAGTATGTTGGCATCTTTATGGTGGCTTTTGCGGTTTTGATTTTCCTATTCCTTGGCTCGGTGGAGAGTTTTAGTACCAAGCCTCAGCCGTGCACATATGACAAAACAAAGATTTGTAAGCCAGCTTTGGCGACGGCTATCTTCAGCACTGTATCATTTTTACTTGGTGCTTTCACTTCAGTGGTTTCTGGTTTCCTTGGAATGAAAATTGCTACATATGCAAATGCCAGAACCACCTTGGAGGCAAGGAAAGGTGTTGGAAAGGCATTTATAACTGCTTTTAGGTCTGGTGCTGTCATGGGCTTCCTCCTAGCTGCCAATGGTCTCTTGGTTTTGTTTATTGCCATTAACCTCTTTAAATTGTACTACGGTGATGATTGGGGTGGCCTTTTTGAGTCTATCACTGGGTATGGTCTTGGTGGATCTTCTATGGCTCTCTTTGGCAGAGTTGGTGGAGGTATCTACACTAAAGCTGCTGATGTTGGTGCTGACCTTGTGGGCAAGGTGGAAAGGAACATTCCTGAGGATGATCCAAGAAATCCAGCTGTAATGATCGTTACTCTAGATATCTTTTCTTAGTTCATTCCGTGCTGAAAATTTTGTCCCTTAATCAAATCACTCATTGCTTTGACAGGTGATTGCTGATAATGTTGGTGATAATGTTGGAGATATAGCTGGTATGGGATCTGATCTTTTTGGTTCATATGCTGAATCGTCCTGTGCTGCCCTAGTTGTTGCTTCTATCTCTTCTTTTGGCAACAACCATGAGTTGACACCAATGCTCTATCCTCTCATCATCAGTTCTGTGGGTATCCTTGTTTGCCTGATCACCACCTTATTTGCTACTGATTTCTTTGAGATCAAGGCTGTTAAGGAAATTGAGCCATCATTGAAGAAGCAGCTCATCATTTCCACTGTTCTAATGACCTTTGGGATTGCAATTGTTACTTGGATTGCTGTTCCATCAACATTCACCATTTTCAATTTTGGAACTCAAAAAGTTGTGAAGAACTGGTTAGTGCTTCTTGAATTATTGTCTTATAATTGCTTTCATTATCACTAGTGGTTTGATTCACCAGCAAATGTTTTTGGCATTCAGGAAACTGTTCTTATGTGTTGCTGTTGGTCTCTGGGCTGGGCTTATAATAGGATTTGTGACAGAGTACTATACGAGCAATGCATACAGGTAATTGATTTATTGCCATCTGTCTAGTTATTCAATTTATAATATAAAAATGAAAATCTGATCACATCTTTGTATGGTTTTTCAGCCCTGTGCAAGATGTTGCTGATTCTTGCCGAACTGGAGCTGCTACAAATGTCATCTTTGGGTTGGCATTGGGATATAAATCTGTCATTATTCCTATTTTTGCAATTGCAGTTAGTATCTTTGTGAGTTTCAGCTTTGCGGCAATGTACGGCATTGCAGTAGCTGCTCTTGGAATGCTGAGTACTATTGCTACTGGTTTGGCCATTGATGCATATGGTCCAATCAGTGACAATGCTGGAGGCATTGCGGAGATGGCAGGCATGAGCCACAGAATTCGGGAGAGAACGGATGCCCTTGATGCTGCAGGAAACACCACTGCAGCCATTGGGAAGGTATTTTTTTTAATTCTTTTTGTCTCAATCCTACTGCATAGATCATTTCTTTATTGGCTGTGGTCTTGGTCTTCGTAGAGTGTCCAGAAGACCATATTGTTTCAATCTACAAATGTTATTTTGTCCAGTTTGCCTCCAAGCTTCCAATGATACTTCAAGTGTGCATGAGAAACTATGATCACACCTTCAGTAGTAAATACAATTTTTTGAACATTTATCAAAATTTGATTCCGTTTTTGCTTTTTCAGGGTTTCGCTATCGGTTCAGCTGCTCTTGTGTCCTTGGCCCTCTTCGGTGCCTTTGTCAGCCGTGCAGGCGTCACTGCTGTTGATGTCTTGACACCCAAAGTTTTCATTGGCTTGATCGTGGGTGCTATGCTGCCATACTGGTTTTCTGCCATGACAATGAAGAGTGTTGGGAGTGCAGCCCTAAAGATGGTTGAGGAGGTGCGAAGGCAATTCAATACCATCCCAGGTCTCATGGAGGGTACTACCAAGCCCGACTATGCTACATGTGTCAAGATCTCAACTGATGCTTCAATCAAAGAGATGATCCCACCCGGTGCCCTCGTCATGTTGACGCCACTAATCGTTGGTATCCTATTTGGTGTCGAAACGCTTTCTGGTGTCCTTGCTGGATCTCTTGTTTCTGGTGTGCAGGTAATGAGCTGATGCCTGGAGCAACTTGTTAACGTTCAATTTTTGCATGAACCTTATAATACCCCACTAACTGATGTTCCTGTTGTACATTAACTGTAGATTGCTATCTCTGCATCCAACACCGGAGGTGCGTGGGATAATGCTAAGAAGTACATCGAGGTATGTCGAATCTAAGTATCATCTTTACAATGTTGTAATGATTTTATGGTATTAGATGTTATAGCAAGTTGGTCTTGTGATCATCATGGACAGGCCGGTGCCTCTGAGCATGCAAGAACCCTCGGGCCGAAAGGATCGGATCCACACAAAGCTGCTGTTATCGGTGACACAATTGGAGACCCACTCAAGGATACATCCGGACCTTCTCTAAACATTCTCATCAAGTTGATGGCTGTGGAGTCCCTTGTGTTTGCACCTTTCTTTGCCAGTCACGGTGGGCTTCTTTTCAAGATCTGA

mRNA sequence

ATGGGCGTCACCATTCTTCCAGATCTCGGGACTCAGATTCTGATTCCGGTTTGTGCCATAGTCGGAATCGTCTTCTCCTTGGTGCAGTGGTACTATGTTTCCCAAGTTAAGCTCTCGCCTGGTCGGGATTCCGCCCATAACAACAGCAGCGCCGGTAAGAACGGCTACTCCGACTACCTTATAGAGGAAGAAGAGGGCGTCAACGACCACAGCGTTGTCATCAAGTGTGCCGAAATTCAAAACGCCATCTCCGAGGGAGCAACCTCCTTCCTTTTCACGGAGTATAAGTATGTTGGCATCTTTATGGTGGCTTTTGCGGTTTTGATTTTCCTATTCCTTGGCTCGGTGGAGAGTTTTAGTACCAAGCCTCAGCCGTGCACATATGACAAAACAAAGATTTGTAAGCCAGCTTTGGCGACGGCTATCTTCAGCACTGTATCATTTTTACTTGGTGCTTTCACTTCAGTGGTTTCTGGTTTCCTTGGAATGAAAATTGCTACATATGCAAATGCCAGAACCACCTTGGAGGCAAGGAAAGGTGTTGGAAAGGCATTTATAACTGCTTTTAGGTCTGGTGCTGTCATGGGCTTCCTCCTAGCTGCCAATGGTCTCTTGGTTTTGTTTATTGCCATTAACCTCTTTAAATTGTACTACGGTGATGATTGGGGTGGCCTTTTTGAGTCTATCACTGGGTATGGTCTTGGTGGATCTTCTATGGCTCTCTTTGGCAGAGTTGGTGGAGGTATCTACACTAAAGCTGCTGATGTTGGTGCTGACCTTGTGGGCAAGGTGGAAAGGAACATTCCTGAGGATGATCCAAGAAATCCAGCTGTGATTGCTGATAATGTTGGTGATAATGTTGGAGATATAGCTGGTATGGGATCTGATCTTTTTGGTTCATATGCTGAATCGTCCTGTGCTGCCCTAGTTGTTGCTTCTATCTCTTCTTTTGGCAACAACCATGAGTTGACACCAATGCTCTATCCTCTCATCATCAGTTCTGTGGGTATCCTTGTTTGCCTGATCACCACCTTATTTGCTACTGATTTCTTTGAGATCAAGGCTGTTAAGGAAATTGAGCCATCATTGAAGAAGCAGCTCATCATTTCCACTGTTCTAATGACCTTTGGGATTGCAATTGTTACTTGGATTGCTGTTCCATCAACATTCACCATTTTCAATTTTGGAACTCAAAAAGTTGTGAAGAACTGGAAACTGTTCTTATGTGTTGCTGTTGGTCTCTGGGCTGGGCTTATAATAGGATTTGTGACAGAGTACTATACGAGCAATGCATACAGCCCTGTGCAAGATGTTGCTGATTCTTGCCGAACTGGAGCTGCTACAAATGTCATCTTTGGGTTGGCATTGGGATATAAATCTGTCATTATTCCTATTTTTGCAATTGCAGTTAGTATCTTTGTGAGTTTCAGCTTTGCGGCAATGTACGGCATTGCAGTAGCTGCTCTTGGAATGCTGAGTACTATTGCTACTGGTTTGGCCATTGATGCATATGGTCCAATCAGTGACAATGCTGGAGGCATTGCGGAGATGGCAGGCATGAGCCACAGAATTCGGGAGAGAACGGATGCCCTTGATGCTGCAGGAAACACCACTGCAGCCATTGGGAAGGGTTTCGCTATCGGTTCAGCTGCTCTTGTGTCCTTGGCCCTCTTCGGTGCCTTTGTCAGCCGTGCAGGCGTCACTGCTGTTGATGTCTTGACACCCAAAGTTTTCATTGGCTTGATCGTGGGTGCTATGCTGCCATACTGGTTTTCTGCCATGACAATGAAGAGTGTTGGGAGTGCAGCCCTAAAGATGGTTGAGGAGGTGCGAAGGCAATTCAATACCATCCCAGGTCTCATGGAGGGTACTACCAAGCCCGACTATGCTACATGTGTCAAGATCTCAACTGATGCTTCAATCAAAGAGATGATCCCACCCGGTGCCCTCGTCATGTTGACGCCACTAATCGTTGGTATCCTATTTGGTGTCGAAACGCTTTCTGGTGTCCTTGCTGGATCTCTTGTTTCTGGTGTGCAGATTGCTATCTCTGCATCCAACACCGGAGGTGCGTGGGATAATGCTAAGAAGTACATCGAGGCCGGTGCCTCTGAGCATGCAAGAACCCTCGGGCCGAAAGGATCGGATCCACACAAAGCTGCTGTTATCGGTGACACAATTGGAGACCCACTCAAGGATACATCCGGACCTTCTCTAAACATTCTCATCAAGTTGATGGCTGTGGAGTCCCTTGTGTTTGCACCTTTCTTTGCCAGTCACGGTGGGCTTCTTTTCAAGATCTGA

Coding sequence (CDS)

ATGGGCGTCACCATTCTTCCAGATCTCGGGACTCAGATTCTGATTCCGGTTTGTGCCATAGTCGGAATCGTCTTCTCCTTGGTGCAGTGGTACTATGTTTCCCAAGTTAAGCTCTCGCCTGGTCGGGATTCCGCCCATAACAACAGCAGCGCCGGTAAGAACGGCTACTCCGACTACCTTATAGAGGAAGAAGAGGGCGTCAACGACCACAGCGTTGTCATCAAGTGTGCCGAAATTCAAAACGCCATCTCCGAGGGAGCAACCTCCTTCCTTTTCACGGAGTATAAGTATGTTGGCATCTTTATGGTGGCTTTTGCGGTTTTGATTTTCCTATTCCTTGGCTCGGTGGAGAGTTTTAGTACCAAGCCTCAGCCGTGCACATATGACAAAACAAAGATTTGTAAGCCAGCTTTGGCGACGGCTATCTTCAGCACTGTATCATTTTTACTTGGTGCTTTCACTTCAGTGGTTTCTGGTTTCCTTGGAATGAAAATTGCTACATATGCAAATGCCAGAACCACCTTGGAGGCAAGGAAAGGTGTTGGAAAGGCATTTATAACTGCTTTTAGGTCTGGTGCTGTCATGGGCTTCCTCCTAGCTGCCAATGGTCTCTTGGTTTTGTTTATTGCCATTAACCTCTTTAAATTGTACTACGGTGATGATTGGGGTGGCCTTTTTGAGTCTATCACTGGGTATGGTCTTGGTGGATCTTCTATGGCTCTCTTTGGCAGAGTTGGTGGAGGTATCTACACTAAAGCTGCTGATGTTGGTGCTGACCTTGTGGGCAAGGTGGAAAGGAACATTCCTGAGGATGATCCAAGAAATCCAGCTGTGATTGCTGATAATGTTGGTGATAATGTTGGAGATATAGCTGGTATGGGATCTGATCTTTTTGGTTCATATGCTGAATCGTCCTGTGCTGCCCTAGTTGTTGCTTCTATCTCTTCTTTTGGCAACAACCATGAGTTGACACCAATGCTCTATCCTCTCATCATCAGTTCTGTGGGTATCCTTGTTTGCCTGATCACCACCTTATTTGCTACTGATTTCTTTGAGATCAAGGCTGTTAAGGAAATTGAGCCATCATTGAAGAAGCAGCTCATCATTTCCACTGTTCTAATGACCTTTGGGATTGCAATTGTTACTTGGATTGCTGTTCCATCAACATTCACCATTTTCAATTTTGGAACTCAAAAAGTTGTGAAGAACTGGAAACTGTTCTTATGTGTTGCTGTTGGTCTCTGGGCTGGGCTTATAATAGGATTTGTGACAGAGTACTATACGAGCAATGCATACAGCCCTGTGCAAGATGTTGCTGATTCTTGCCGAACTGGAGCTGCTACAAATGTCATCTTTGGGTTGGCATTGGGATATAAATCTGTCATTATTCCTATTTTTGCAATTGCAGTTAGTATCTTTGTGAGTTTCAGCTTTGCGGCAATGTACGGCATTGCAGTAGCTGCTCTTGGAATGCTGAGTACTATTGCTACTGGTTTGGCCATTGATGCATATGGTCCAATCAGTGACAATGCTGGAGGCATTGCGGAGATGGCAGGCATGAGCCACAGAATTCGGGAGAGAACGGATGCCCTTGATGCTGCAGGAAACACCACTGCAGCCATTGGGAAGGGTTTCGCTATCGGTTCAGCTGCTCTTGTGTCCTTGGCCCTCTTCGGTGCCTTTGTCAGCCGTGCAGGCGTCACTGCTGTTGATGTCTTGACACCCAAAGTTTTCATTGGCTTGATCGTGGGTGCTATGCTGCCATACTGGTTTTCTGCCATGACAATGAAGAGTGTTGGGAGTGCAGCCCTAAAGATGGTTGAGGAGGTGCGAAGGCAATTCAATACCATCCCAGGTCTCATGGAGGGTACTACCAAGCCCGACTATGCTACATGTGTCAAGATCTCAACTGATGCTTCAATCAAAGAGATGATCCCACCCGGTGCCCTCGTCATGTTGACGCCACTAATCGTTGGTATCCTATTTGGTGTCGAAACGCTTTCTGGTGTCCTTGCTGGATCTCTTGTTTCTGGTGTGCAGATTGCTATCTCTGCATCCAACACCGGAGGTGCGTGGGATAATGCTAAGAAGTACATCGAGGCCGGTGCCTCTGAGCATGCAAGAACCCTCGGGCCGAAAGGATCGGATCCACACAAAGCTGCTGTTATCGGTGACACAATTGGAGACCCACTCAAGGATACATCCGGACCTTCTCTAAACATTCTCATCAAGTTGATGGCTGTGGAGTCCCTTGTGTTTGCACCTTTCTTTGCCAGTCACGGTGGGCTTCTTTTCAAGATCTGA

Protein sequence

MGVTILPDLGTQILIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNSSAGKNGYSDYLIEEEEGVNDHSVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVESFSTKPQPCTYDKTKICKPALATAIFSTVSFLLGAFTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAALVVASISSFGNNHELTPMLYPLIISSVGILVCLITTLFATDFFEIKAVKEIEPSLKKQLIISTVLMTFGIAIVTWIAVPSTFTIFNFGTQKVVKNWKLFLCVAVGLWAGLIIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFSFAAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEEVRRQFNTIPGLMEGTTKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI
Homology
BLAST of Sgr027089 vs. NCBI nr
Match: XP_022929683.1 (pyrophosphate-energized vacuolar membrane proton pump-like [Cucurbita moschata] >KAG6587868.1 hypothetical protein SDJN03_16433, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021755.1 hypothetical protein SDJN02_15482 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1408.3 bits (3644), Expect = 0.0e+00
Identity = 743/767 (96.87%), Postives = 758/767 (98.83%), Query Frame = 0

Query: 1   MGVTILPDLGTQILIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNSSAGKNGYSDYL 60
           M VTILPDLGT+I IPVCA+VGIVFSLVQWYYVSQVKLSPGRD+AHNNS+  KNGYSDYL
Sbjct: 1   MSVTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60

Query: 61  IEEEEGVNDHSVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVESFS 120
           IEEEEGVNDH+VVIKCAEIQ+AISEGATSFLFTEYKYVGIFMV FAVLIF+FLGSVESFS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120

Query: 121 TKPQPCTYDKTKICKPALATAIFSTVSFLLGAFTSVVSGFLGMKIATYANARTTLEARKG 180
           TKPQPCTYDKT+ CKPALATAIFSTVSFLLGA TSVVSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIISSVGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFGNNHELTPMLYPLI+SS+GILVCLITTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360

Query: 361 PSLKKQLIISTVLMTFGIAIVTWIAVPSTFTIFNFGTQKVVKNWKLFLCVAVGLWAGLII 420
           P+LKKQLIISTVLMTFGIAIVTW++VPS+FTIFNFGTQKVV NWKLFLCVAVGLWAGLII
Sbjct: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFSFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSF+FAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTTKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGT KPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 768
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 767

BLAST of Sgr027089 vs. NCBI nr
Match: XP_023002108.1 (pyrophosphate-energized vacuolar membrane proton pump-like [Cucurbita maxima])

HSP 1 Score: 1407.5 bits (3642), Expect = 0.0e+00
Identity = 741/767 (96.61%), Postives = 758/767 (98.83%), Query Frame = 0

Query: 1   MGVTILPDLGTQILIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNSSAGKNGYSDYL 60
           M VTILPDLGT+I IPVCA++GI+FSLVQWYYVSQVKLSPGRD+AHNNS+  KNGYSDYL
Sbjct: 1   MSVTILPDLGTEIFIPVCAVIGIIFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60

Query: 61  IEEEEGVNDHSVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVESFS 120
           IEEEEGVNDH+VVIKCAEIQ+AISEGATSFLFTEYKYVGIFMV FAVLIF+FLGSVESFS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120

Query: 121 TKPQPCTYDKTKICKPALATAIFSTVSFLLGAFTSVVSGFLGMKIATYANARTTLEARKG 180
           TKPQPCTYDKT+ CKPALATAIFSTVSFLLGA TSVVSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIISSVGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFGNNHELTPMLYPLI+SS+GILVCLITTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360

Query: 361 PSLKKQLIISTVLMTFGIAIVTWIAVPSTFTIFNFGTQKVVKNWKLFLCVAVGLWAGLII 420
           P+LKKQLIISTVLMTFGIAIVTW++VPS+FTIFNFGTQKVV NWKLFLCVAVGLWAGLII
Sbjct: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFSFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSF+FAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTTKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGT KPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 768
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 767

BLAST of Sgr027089 vs. NCBI nr
Match: XP_023529940.1 (pyrophosphate-energized vacuolar membrane proton pump-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1407.1 bits (3641), Expect = 0.0e+00
Identity = 744/767 (97.00%), Postives = 757/767 (98.70%), Query Frame = 0

Query: 1   MGVTILPDLGTQILIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNSSAGKNGYSDYL 60
           M VTILPDLGT+I IPVCA+VGIVFSLVQWYYVSQVKLSPGRD+AHNNS+  KNGYSDYL
Sbjct: 1   MSVTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60

Query: 61  IEEEEGVNDHSVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVESFS 120
           IEEEEGVNDH+VVIKCAEIQ AISEGATSFLFTEYKYVGIFMV FAVLIF+FLGSVESFS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQCAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120

Query: 121 TKPQPCTYDKTKICKPALATAIFSTVSFLLGAFTSVVSGFLGMKIATYANARTTLEARKG 180
           TKPQPCTYDKT+ CKPALATAIFSTVSFLLGA TSVVSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIISSVGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFGNNHELTPMLYPLIISS+GILVCLITTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIISSMGILVCLITTLFATDFFEIKAVKEIE 360

Query: 361 PSLKKQLIISTVLMTFGIAIVTWIAVPSTFTIFNFGTQKVVKNWKLFLCVAVGLWAGLII 420
           P+LKKQLIISTVLMTFGIAIVTW++VPS+FTIFNFGTQKVV NWKLFLCVAVGLWAGLII
Sbjct: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFSFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSF+FAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTTKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGT KPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 768
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 767

BLAST of Sgr027089 vs. NCBI nr
Match: XP_022135071.1 (pyrophosphate-energized vacuolar membrane proton pump [Momordica charantia])

HSP 1 Score: 1406.0 bits (3638), Expect = 0.0e+00
Identity = 743/769 (96.62%), Postives = 759/769 (98.70%), Query Frame = 0

Query: 1   MGVTILPDLGTQILIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNS--SAGKNGYSD 60
           MGVTILPDLG +ILIPVC+++GIVFSL+QWYYVSQVKLSP RDSAHNNS  +A KNGYSD
Sbjct: 1   MGVTILPDLGAEILIPVCSVIGIVFSLLQWYYVSQVKLSPARDSAHNNSAAAAAKNGYSD 60

Query: 61  YLIEEEEGVNDHSVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVES 120
           YLIEEEEGVNDH+VVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVES
Sbjct: 61  YLIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVES 120

Query: 121 FSTKPQPCTYDKTKICKPALATAIFSTVSFLLGAFTSVVSGFLGMKIATYANARTTLEAR 180
           FSTKPQPC+YDK+K CKPALATAIFST+SFLLGAFTSVVSGFLGMKIATYANARTTLEAR
Sbjct: 121 FSTKPQPCSYDKSKTCKPALATAIFSTISFLLGAFTSVVSGFLGMKIATYANARTTLEAR 180

Query: 181 KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS 240
           KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS
Sbjct: 181 KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS 240

Query: 241 MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF 300
           MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF
Sbjct: 241 MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF 300

Query: 301 GSYAESSCAALVVASISSFGNNHELTPMLYPLIISSVGILVCLITTLFATDFFEIKAVKE 360
           GSYAESSCAALVVASISSFGNNHELTPMLYPLI+SSVGILVCL+TTLFATDFFEIKAVKE
Sbjct: 301 GSYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKE 360

Query: 361 IEPSLKKQLIISTVLMTFGIAIVTWIAVPSTFTIFNFGTQKVVKNWKLFLCVAVGLWAGL 420
           IEP+LKKQLIISTVLMTFG+AIVTWI+VPSTFTIFNFGTQK V NWKLFLCVAVGLWAGL
Sbjct: 361 IEPALKKQLIISTVLMTFGVAIVTWISVPSTFTIFNFGTQKDVTNWKLFLCVAVGLWAGL 420

Query: 421 IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFSF 480
           IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSF+F
Sbjct: 421 IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF 480

Query: 481 AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT 540
           AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT
Sbjct: 481 AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT 540

Query: 541 AAIGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKS 600
           AAIGKGFAIGSAALVSLALFGAFVSRAGV AVDVLTPKVFIGLIVGAMLPYWFSAMTMKS
Sbjct: 541 AAIGKGFAIGSAALVSLALFGAFVSRAGVNAVDVLTPKVFIGLIVGAMLPYWFSAMTMKS 600

Query: 601 VGSAALKMVEEVRRQFNTIPGLMEGTTKPDYATCVKISTDASIKEMIPPGALVMLTPLIV 660
           VGSAALKMVEEVRRQFNTIPGLMEGT KPDYATCVKISTDASIKEMIPPGALVMLTPLIV
Sbjct: 601 VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV 660

Query: 661 GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH 720
           GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH
Sbjct: 661 GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH 720

Query: 721 KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 768
           KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI
Sbjct: 721 KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 769

BLAST of Sgr027089 vs. NCBI nr
Match: XP_023515840.1 (pyrophosphate-energized vacuolar membrane proton pump [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1404.8 bits (3635), Expect = 0.0e+00
Identity = 740/765 (96.73%), Postives = 757/765 (98.95%), Query Frame = 0

Query: 3   VTILPDLGTQILIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNSSAGKNGYSDYLIE 62
           VTILPDLGTQI IPVCA+VGIVFSLVQWYYVSQVKLSPGRDS HNNS+A KNGYSDYLIE
Sbjct: 4   VTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDSTHNNSAAAKNGYSDYLIE 63

Query: 63  EEEGVNDHSVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVESFSTK 122
           EEEGVNDH+VVIKCAEIQNAISEGATSFLFTEYKYVGIFMV FAVLIF+FLGSVE FST+
Sbjct: 64  EEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGFSTE 123

Query: 123 PQPCTYDKTKICKPALATAIFSTVSFLLGAFTSVVSGFLGMKIATYANARTTLEARKGVG 182
           PQ CTYDKTKICKPALATAIFST+SFLLGA TSVVSGFLGMKIATYANARTTLEARKGVG
Sbjct: 124 PQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVG 183

Query: 183 KAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALF 242
           KAFITAFRSGAVMGFLLAANGLLVLFIAIN+FKLYYG+DWGGLFESITGYGLGGSSMALF
Sbjct: 184 KAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSMALF 243

Query: 243 GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA 302
           GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA
Sbjct: 244 GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA 303

Query: 303 ESSCAALVVASISSFGNNHELTPMLYPLIISSVGILVCLITTLFATDFFEIKAVKEIEPS 362
           ESSCAALVVASISSFGNNHELTPMLYPLI+SSVGILVCLITTLFATDFFEIKAVKEIEP+
Sbjct: 304 ESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEIEPA 363

Query: 363 LKKQLIISTVLMTFGIAIVTWIAVPSTFTIFNFGTQKVVKNWKLFLCVAVGLWAGLIIGF 422
           LKKQLIISTVLMTFGIAIVTW+AVPSTFTIFNFGTQKVV+NWKLFLCVAVGLWAGLIIGF
Sbjct: 364 LKKQLIISTVLMTFGIAIVTWVAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLIIGF 423

Query: 423 VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFSFAAMY 482
           VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSF+FAAMY
Sbjct: 424 VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMY 483

Query: 483 GIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG 542
           GIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG
Sbjct: 484 GIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG 543

Query: 543 KGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSA 602
           KGFAIGSAALVSLALFGAFVSRAGVT+VDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSA
Sbjct: 544 KGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSA 603

Query: 603 ALKMVEEVRRQFNTIPGLMEGTTKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILF 662
           ALKMVEEVRRQFNTIPGLMEGT KPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILF
Sbjct: 604 ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILF 663

Query: 663 GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAV 722
           GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAV
Sbjct: 664 GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAV 723

Query: 723 IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 768
           IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA+HGGLLF++
Sbjct: 724 IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFRL 768

BLAST of Sgr027089 vs. ExPASy Swiss-Prot
Match: P21616 (Pyrophosphate-energized vacuolar membrane proton pump OS=Vigna radiata var. radiata OX=3916 PE=1 SV=4)

HSP 1 Score: 1333.9 bits (3451), Expect = 0.0e+00
Identity = 702/767 (91.53%), Postives = 740/767 (96.48%), Query Frame = 0

Query: 1   MGVTILPDLGTQILIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNSSAGKNGYSDYL 60
           MG  ILPDLGT+ILIPVCA++GI F+L QW  VS+VKLS  RD++ N  +A KNGY+DYL
Sbjct: 1   MGAAILPDLGTEILIPVCAVIGIAFALFQWLLVSKVKLSAVRDASPN--AAAKNGYNDYL 60

Query: 61  IEEEEGVNDHSVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVESFS 120
           IEEEEG+NDH+VV+KCAEIQNAISEGATSFLFTEYKYVGIFMVAFA+LIFLFLGSVE FS
Sbjct: 61  IEEEEGINDHNVVVKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAILIFLFLGSVEGFS 120

Query: 121 TKPQPCTYDKTKICKPALATAIFSTVSFLLGAFTSVVSGFLGMKIATYANARTTLEARKG 180
           T PQ C+YDKTK CKPALATAIFSTVSFLLG  TS+VSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TSPQACSYDKTKTCKPALATAIFSTVSFLLGGVTSLVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVL+IAINLFK+YYGDDWGGLFE+ITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLYIAINLFKIYYGDDWGGLFEAITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIISSVGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFG NHELT MLYPLI+SSVGILVCL+TTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGLNHELTAMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKEIE 360

Query: 361 PSLKKQLIISTVLMTFGIAIVTWIAVPSTFTIFNFGTQKVVKNWKLFLCVAVGLWAGLII 420
           P+LKKQL+ISTVLMT G+A+V+++A+P++FTIFNFG QK VK+W+LFLCVAVGLWAGLII
Sbjct: 361 PALKKQLVISTVLMTIGVAVVSFVALPTSFTIFNFGVQKDVKSWQLFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFSFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA+SIFVSF+FAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFTFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRA +T VDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRASITTVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTTKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGT KPDYATCVKISTDASIKEMIPPGALVMLTPL+VGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLVVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHAR+LGPKGSD HKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARSLGPKGSDCHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 768
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA+HGGLLFKI
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFKI 765

BLAST of Sgr027089 vs. ExPASy Swiss-Prot
Match: P31414 (Pyrophosphate-energized vacuolar membrane proton pump 1 OS=Arabidopsis thaliana OX=3702 GN=AVP1 PE=1 SV=1)

HSP 1 Score: 1280.0 bits (3311), Expect = 0.0e+00
Identity = 668/763 (87.55%), Postives = 717/763 (93.97%), Query Frame = 0

Query: 5   ILPDLGTQILIPVCAIVGIVFSLVQWYYVSQVKLSPGRD-SAHNNSSAGKNGYSDYLIEE 64
           +LP+L T+IL+P+CA++GI FSL QWY VS+VKL+     S+   ++ GKNGY DYLIEE
Sbjct: 6   LLPELWTEILVPICAVIGIAFSLFQWYVVSRVKLTSDLGASSSGGANNGKNGYGDYLIEE 65

Query: 65  EEGVNDHSVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVESFSTKP 124
           EEGVND SVV KCAEIQ AISEGATSFLFTEYKYVG+FM+ FA +IF+FLGSVE FST  
Sbjct: 66  EEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEGFSTDN 125

Query: 125 QPCTYDKTKICKPALATAIFSTVSFLLGAFTSVVSGFLGMKIATYANARTTLEARKGVGK 184
           +PCTYD T+ CKPALATA FST++F+LGA TSV+SGFLGMKIATYANARTTLEARKGVGK
Sbjct: 126 KPCTYDTTRTCKPALATAAFSTIAFVLGAVTSVLSGFLGMKIATYANARTTLEARKGVGK 185

Query: 185 AFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALFG 244
           AFI AFRSGAVMGFLLAA+GLLVL+I IN+FK+YYGDDW GLFE+ITGYGLGGSSMALFG
Sbjct: 186 AFIVAFRSGAVMGFLLAASGLLVLYITINVFKIYYGDDWEGLFEAITGYGLGGSSMALFG 245

Query: 245 RVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 304
           RVGGGIYTKAADVGADLVGK+ERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE
Sbjct: 246 RVGGGIYTKAADVGADLVGKIERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 305

Query: 305 SSCAALVVASISSFGNNHELTPMLYPLIISSVGILVCLITTLFATDFFEIKAVKEIEPSL 364
           +SCAALVVASISSFG NH+ T M YPL+ISS+GILVCLITTLFATDFFEIK VKEIEP+L
Sbjct: 306 ASCAALVVASISSFGINHDFTAMCYPLLISSMGILVCLITTLFATDFFEIKLVKEIEPAL 365

Query: 365 KKQLIISTVLMTFGIAIVTWIAVPSTFTIFNFGTQKVVKNWKLFLCVAVGLWAGLIIGFV 424
           K QLIISTV+MT GIAIV+W+ +P++FTIFNFGTQKVVKNW+LFLCV VGLWAGLIIGFV
Sbjct: 366 KNQLIISTVIMTVGIAIVSWVGLPTSFTIFNFGTQKVVKNWQLFLCVCVGLWAGLIIGFV 425

Query: 425 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFSFAAMYG 484
           TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA+SIFVSFSFAAMYG
Sbjct: 426 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFSFAAMYG 485

Query: 485 IAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 544
           +AVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK
Sbjct: 486 VAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 545

Query: 545 GFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAA 604
           GFAIGSAALVSLALFGAFVSRAG+  VDVLTPKV IGL+VGAMLPYWFSAMTMKSVGSAA
Sbjct: 546 GFAIGSAALVSLALFGAFVSRAGIHTVDVLTPKVIIGLLVGAMLPYWFSAMTMKSVGSAA 605

Query: 605 LKMVEEVRRQFNTIPGLMEGTTKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFG 664
           LKMVEEVRRQFNTIPGLMEGT KPDYATCVKISTDASIKEMIPPG LVMLTPLIVG  FG
Sbjct: 606 LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGCLVMLTPLIVGFFFG 665

Query: 665 VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVI 724
           VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAG SEHA++LGPKGS+PHKAAVI
Sbjct: 666 VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGVSEHAKSLGPKGSEPHKAAVI 725

Query: 725 GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFK 767
           GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA+HGG+LFK
Sbjct: 726 GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGILFK 768

BLAST of Sgr027089 vs. ExPASy Swiss-Prot
Match: Q06572 (Pyrophosphate-energized vacuolar membrane proton pump OS=Hordeum vulgare OX=4513 PE=2 SV=2)

HSP 1 Score: 1265.0 bits (3272), Expect = 0.0e+00
Identity = 665/764 (87.04%), Postives = 715/764 (93.59%), Query Frame = 0

Query: 3   VTILPDLGTQILIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNSSAGKNGYSDYLIE 62
           + IL +LGT+ILIPVC ++GIVF++ QW+ VS+VK++PG  SA   ++  KNGY DYLIE
Sbjct: 1   MAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPGAASA---AAGAKNGYGDYLIE 60

Query: 63  EEEGVNDHSVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVESFSTK 122
           EEEG+NDH+VV+KCAEIQ AISEGATSFLFT Y+YVG+FMV FA +IFLFLGS+E FSTK
Sbjct: 61  EEEGLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGFSTK 120

Query: 123 PQPCTYDKTKICKPALATAIFSTVSFLLGAFTSVVSGFLGMKIATYANARTTLEARKGVG 182
            QPCTY K   CKPAL TA+FST SFLLGA TS+VSGFLGMKIATYANARTTLEARKGVG
Sbjct: 121 GQPCTYSK-GTCKPALYTALFSTASFLLGAITSLVSGFLGMKIATYANARTTLEARKGVG 180

Query: 183 KAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALF 242
           KAFITAFRSGAVMGFLL+++GL+VL+I IN+FK+YYGDDW GLFESITGYGLGGSSMALF
Sbjct: 181 KAFITAFRSGAVMGFLLSSSGLVVLYITINVFKMYYGDDWEGLFESITGYGLGGSSMALF 240

Query: 243 GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA 302
           GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA
Sbjct: 241 GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA 300

Query: 303 ESSCAALVVASISSFGNNHELTPMLYPLIISSVGILVCLITTLFATDFFEIKAVKEIEPS 362
           ESSCAALVVASISSFG NH+ T M YPL++SSVGI+VCL+TTLFATDFFEIKA  EIEP+
Sbjct: 301 ESSCAALVVASISSFGINHDFTAMCYPLLVSSVGIIVCLLTTLFATDFFEIKAANEIEPA 360

Query: 363 LKKQLIISTVLMTFGIAIVTWIAVPSTFTIFNFGTQKVVKNWKLFLCVAVGLWAGLIIGF 422
           LKKQLIIST LMT G+A+++W+A+P+ FTIFNFG QK V NW LF CVAVGLWAGLIIGF
Sbjct: 361 LKKQLIISTALMTVGVAVISWLALPAKFTIFNFGAQKEVSNWGLFFCVAVGLWAGLIIGF 420

Query: 423 VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFSFAAMY 482
           VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSI+VSFS AAMY
Sbjct: 421 VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIYVSFSIAAMY 480

Query: 483 GIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG 542
           GIA+AALGMLST+ATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG
Sbjct: 481 GIAMAALGMLSTMATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG 540

Query: 543 KGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSA 602
           KGFAIGSAALVSLALFGAFVSRAGV  VDVL+PKVFIGLIVGAMLPYWFSAMTMKSVGSA
Sbjct: 541 KGFAIGSAALVSLALFGAFVSRAGVKVVDVLSPKVFIGLIVGAMLPYWFSAMTMKSVGSA 600

Query: 603 ALKMVEEVRRQFNTIPGLMEGTTKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILF 662
           ALKMVEEVRRQFNTIPGLMEGT KPDYATCVKISTDASIKEMIPPGALVMLTPLIVG LF
Sbjct: 601 ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGTLF 660

Query: 663 GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAV 722
           GVETLSGVLAG+LVSGVQIAISASNTGGAWDNAKKYIEAG SEHAR+LGPKGSD HKAAV
Sbjct: 661 GVETLSGVLAGALVSGVQIAISASNTGGAWDNAKKYIEAGNSEHARSLGPKGSDCHKAAV 720

Query: 723 IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFK 767
           IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA++GGLLFK
Sbjct: 721 IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATYGGLLFK 760

BLAST of Sgr027089 vs. ExPASy Swiss-Prot
Match: Q72Q29 (Putative K(+)-stimulated pyrophosphate-energized sodium pump OS=Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain Fiocruz L1-130) OX=267671 GN=hppA PE=3 SV=1)

HSP 1 Score: 694.5 bits (1791), Expect = 1.3e-198
Identity = 395/696 (56.75%), Postives = 503/696 (72.27%), Query Frame = 0

Query: 75  KCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVESFSTKPQPCTYDKTKIC 134
           K  EI +AISEGA +FL  EYK + +F+   AVLI L L +  S                
Sbjct: 42  KLLEISSAISEGAMAFLVREYKVISLFIAFMAVLIVLLLDNPGS---------------- 101

Query: 135 KPALATAIFSTVSFLLGAFTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAV 194
                  I++ ++F+ GA  S +SGF+GMKIAT  N RT   A+  + KAF  AF SGAV
Sbjct: 102 -EGFNDGIYTAIAFVSGALISCISGFIGMKIATAGNVRTAEAAKSSMAKAFRVAFDSGAV 161

Query: 195 MGF---LLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALFGRVGGGIYT 254
           MGF    LA  G++VLF+      +Y G +   L ES+ G+GLGGS++ALFGRVGGGIYT
Sbjct: 162 MGFGLVGLAILGMIVLFLVFT--GMYPGVEKHFLMESLAGFGLGGSAVALFGRVGGGIYT 221

Query: 255 KAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAALVV 314
           KAADVGADLVGKVE+ IPEDDPRNPA IADNVGDNVGD+AGMG+DLFGS AE++CAALV+
Sbjct: 222 KAADVGADLVGKVEKGIPEDDPRNPATIADNVGDNVGDVAGMGADLFGSCAEATCAALVI 281

Query: 315 ASISSFGNNHELTPMLYPLIISSVGILVCLITTLFATDFFEIKAVKEIEPSLKKQLIIST 374
            + +S   +  +  +LYPL+IS+ GI   ++T+  A     +K    +E +LK QL +ST
Sbjct: 282 GATAS-ALSGSVDALLYPLLISAFGIPASILTSFLA----RVKEDGNVESALKVQLWVST 341

Query: 375 VLMTFGIAIVTWIAVPSTFTIFNFGTQKVVKNWKLFLCVAVGLWAGLIIGFVTEYYTSNA 434
           +L+   +  VT   +  +F I      K +  W +++ + VGL++G+ IG VTEYYTS++
Sbjct: 342 LLVAGIMYFVTKTFMVDSFEI----AGKTITKWDVYISMVVGLFSGMFIGIVTEYYTSHS 401

Query: 435 YSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFSFAAMYGIAVAALGM 494
           Y PV++VA++  TGAATN+I+GL+LGY S +IP+  + ++I  +   A MYGIA+AALGM
Sbjct: 402 YKPVREVAEASNTGAATNIIYGLSLGYHSSVIPVILLVITIVTANLLAGMYGIAIAALGM 461

Query: 495 LSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKGFAIGSAA 554
           +STIA GL IDAYGP+SDNAGGIAEMA +   +R+RTD LDAAGNTTAAIGKGFAIGSAA
Sbjct: 462 ISTIAIGLTIDAYGPVSDNAGGIAEMAELGKEVRDRTDTLDAAGNTTAAIGKGFAIGSAA 521

Query: 555 LVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEEVR 614
           L SLALF AF++R   T+++VL  +VF GL+ GAMLP+ F+AMTMKSVG AA+ MVEEVR
Sbjct: 522 LTSLALFAAFITRTHTTSLEVLNAEVFGGLMFGAMLPFLFTAMTMKSVGKAAVDMVEEVR 581

Query: 615 RQFNTIPGLMEGTTKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFGVETLSGVL 674
           +QF  IPG+MEG  KPDY  CV IST A+++EMI PG LV+LTP++VG LFGV+TL+GVL
Sbjct: 582 KQFKEIPGIMEGKNKPDYKRCVDISTSAALREMILPGLLVLLTPILVGYLFGVKTLAGVL 641

Query: 675 AGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPL 734
           AG+LV+GV +AISA+N+GG WDNAKKYIE  A       G KGSD HKAAV+GDT+GDP 
Sbjct: 642 AGALVAGVVLAISAANSGGGWDNAKKYIEKKA-------GGKGSDQHKAAVVGDTVGDPF 701

Query: 735 KDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 768
           KDTSGPS+NILIKLMA+ SLVFA FF   GGL+FKI
Sbjct: 702 KDTSGPSINILIKLMAITSLVFAEFFVQQGGLIFKI 702

BLAST of Sgr027089 vs. ExPASy Swiss-Prot
Match: Q8F641 (Putative K(+)-stimulated pyrophosphate-energized sodium pump OS=Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) OX=189518 GN=hppA PE=3 SV=1)

HSP 1 Score: 694.5 bits (1791), Expect = 1.3e-198
Identity = 395/696 (56.75%), Postives = 503/696 (72.27%), Query Frame = 0

Query: 75  KCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVESFSTKPQPCTYDKTKIC 134
           K  EI +AISEGA +FL  EYK + +F+   AVLI L L +  S                
Sbjct: 42  KLLEISSAISEGAMAFLVREYKVISLFIAFMAVLIVLLLDNPGS---------------- 101

Query: 135 KPALATAIFSTVSFLLGAFTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAV 194
                  I++ ++F+ GA  S +SGF+GMKIAT  N RT   A+  + KAF  AF SGAV
Sbjct: 102 -EGFNDGIYTAIAFVSGALISCISGFIGMKIATAGNVRTAEAAKSSMAKAFRVAFDSGAV 161

Query: 195 MGF---LLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALFGRVGGGIYT 254
           MGF    LA  G++VLF+      +Y G +   L ES+ G+GLGGS++ALFGRVGGGIYT
Sbjct: 162 MGFGLVGLAILGMIVLFLVFT--GMYPGVEKHFLMESLAGFGLGGSAVALFGRVGGGIYT 221

Query: 255 KAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAALVV 314
           KAADVGADLVGKVE+ IPEDDPRNPA IADNVGDNVGD+AGMG+DLFGS AE++CAALV+
Sbjct: 222 KAADVGADLVGKVEKGIPEDDPRNPATIADNVGDNVGDVAGMGADLFGSCAEATCAALVI 281

Query: 315 ASISSFGNNHELTPMLYPLIISSVGILVCLITTLFATDFFEIKAVKEIEPSLKKQLIIST 374
            + +S   +  +  +LYPL+IS+ GI   ++T+  A     +K    +E +LK QL +ST
Sbjct: 282 GATAS-ALSGSVDALLYPLLISAFGIPASILTSFLA----RVKEDGNVESALKVQLWVST 341

Query: 375 VLMTFGIAIVTWIAVPSTFTIFNFGTQKVVKNWKLFLCVAVGLWAGLIIGFVTEYYTSNA 434
           +L+   +  VT   +  +F I      K +  W +++ + VGL++G+ IG VTEYYTS++
Sbjct: 342 LLVAGIMYFVTKTFMVDSFEI----AGKTITKWDVYISMVVGLFSGMFIGIVTEYYTSHS 401

Query: 435 YSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFSFAAMYGIAVAALGM 494
           Y PV++VA++  TGAATN+I+GL+LGY S +IP+  + ++I  +   A MYGIA+AALGM
Sbjct: 402 YKPVREVAEASNTGAATNIIYGLSLGYHSSVIPVILLVITIVTANLLAGMYGIAIAALGM 461

Query: 495 LSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKGFAIGSAA 554
           +STIA GL IDAYGP+SDNAGGIAEMA +   +R+RTD LDAAGNTTAAIGKGFAIGSAA
Sbjct: 462 ISTIAIGLTIDAYGPVSDNAGGIAEMAELGKEVRDRTDTLDAAGNTTAAIGKGFAIGSAA 521

Query: 555 LVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEEVR 614
           L SLALF AF++R   T+++VL  +VF GL+ GAMLP+ F+AMTMKSVG AA+ MVEEVR
Sbjct: 522 LTSLALFAAFITRTHTTSLEVLNAEVFGGLMFGAMLPFLFTAMTMKSVGKAAVDMVEEVR 581

Query: 615 RQFNTIPGLMEGTTKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFGVETLSGVL 674
           +QF  IPG+MEG  KPDY  CV IST A+++EMI PG LV+LTP++VG LFGV+TL+GVL
Sbjct: 582 KQFKEIPGIMEGKNKPDYKRCVDISTSAALREMILPGLLVLLTPILVGYLFGVKTLAGVL 641

Query: 675 AGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPL 734
           AG+LV+GV +AISA+N+GG WDNAKKYIE  A       G KGSD HKAAV+GDT+GDP 
Sbjct: 642 AGALVAGVVLAISAANSGGGWDNAKKYIEKKA-------GGKGSDQHKAAVVGDTVGDPF 701

Query: 735 KDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 768
           KDTSGPS+NILIKLMA+ SLVFA FF   GGL+FKI
Sbjct: 702 KDTSGPSINILIKLMAITSLVFAEFFVQQGGLIFKI 702

BLAST of Sgr027089 vs. ExPASy TrEMBL
Match: A0A6J1ESX2 (H(+)-exporting diphosphatase OS=Cucurbita moschata OX=3662 GN=LOC111436191 PE=3 SV=1)

HSP 1 Score: 1408.3 bits (3644), Expect = 0.0e+00
Identity = 743/767 (96.87%), Postives = 758/767 (98.83%), Query Frame = 0

Query: 1   MGVTILPDLGTQILIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNSSAGKNGYSDYL 60
           M VTILPDLGT+I IPVCA+VGIVFSLVQWYYVSQVKLSPGRD+AHNNS+  KNGYSDYL
Sbjct: 1   MSVTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60

Query: 61  IEEEEGVNDHSVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVESFS 120
           IEEEEGVNDH+VVIKCAEIQ+AISEGATSFLFTEYKYVGIFMV FAVLIF+FLGSVESFS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120

Query: 121 TKPQPCTYDKTKICKPALATAIFSTVSFLLGAFTSVVSGFLGMKIATYANARTTLEARKG 180
           TKPQPCTYDKT+ CKPALATAIFSTVSFLLGA TSVVSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIISSVGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFGNNHELTPMLYPLI+SS+GILVCLITTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360

Query: 361 PSLKKQLIISTVLMTFGIAIVTWIAVPSTFTIFNFGTQKVVKNWKLFLCVAVGLWAGLII 420
           P+LKKQLIISTVLMTFGIAIVTW++VPS+FTIFNFGTQKVV NWKLFLCVAVGLWAGLII
Sbjct: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFSFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSF+FAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTTKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGT KPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 768
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 767

BLAST of Sgr027089 vs. ExPASy TrEMBL
Match: A0A6J1KSL0 (H(+)-exporting diphosphatase OS=Cucurbita maxima OX=3661 GN=LOC111496068 PE=3 SV=1)

HSP 1 Score: 1407.5 bits (3642), Expect = 0.0e+00
Identity = 741/767 (96.61%), Postives = 758/767 (98.83%), Query Frame = 0

Query: 1   MGVTILPDLGTQILIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNSSAGKNGYSDYL 60
           M VTILPDLGT+I IPVCA++GI+FSLVQWYYVSQVKLSPGRD+AHNNS+  KNGYSDYL
Sbjct: 1   MSVTILPDLGTEIFIPVCAVIGIIFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60

Query: 61  IEEEEGVNDHSVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVESFS 120
           IEEEEGVNDH+VVIKCAEIQ+AISEGATSFLFTEYKYVGIFMV FAVLIF+FLGSVESFS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120

Query: 121 TKPQPCTYDKTKICKPALATAIFSTVSFLLGAFTSVVSGFLGMKIATYANARTTLEARKG 180
           TKPQPCTYDKT+ CKPALATAIFSTVSFLLGA TSVVSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIISSVGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASISSFGNNHELTPMLYPLI+SS+GILVCLITTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360

Query: 361 PSLKKQLIISTVLMTFGIAIVTWIAVPSTFTIFNFGTQKVVKNWKLFLCVAVGLWAGLII 420
           P+LKKQLIISTVLMTFGIAIVTW++VPS+FTIFNFGTQKVV NWKLFLCVAVGLWAGLII
Sbjct: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFSFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSF+FAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTTKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGT KPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 768
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 767

BLAST of Sgr027089 vs. ExPASy TrEMBL
Match: A0A6J1BZK8 (H(+)-exporting diphosphatase OS=Momordica charantia OX=3673 GN=LOC111007149 PE=3 SV=1)

HSP 1 Score: 1406.0 bits (3638), Expect = 0.0e+00
Identity = 743/769 (96.62%), Postives = 759/769 (98.70%), Query Frame = 0

Query: 1   MGVTILPDLGTQILIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNS--SAGKNGYSD 60
           MGVTILPDLG +ILIPVC+++GIVFSL+QWYYVSQVKLSP RDSAHNNS  +A KNGYSD
Sbjct: 1   MGVTILPDLGAEILIPVCSVIGIVFSLLQWYYVSQVKLSPARDSAHNNSAAAAAKNGYSD 60

Query: 61  YLIEEEEGVNDHSVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVES 120
           YLIEEEEGVNDH+VVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVES
Sbjct: 61  YLIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVES 120

Query: 121 FSTKPQPCTYDKTKICKPALATAIFSTVSFLLGAFTSVVSGFLGMKIATYANARTTLEAR 180
           FSTKPQPC+YDK+K CKPALATAIFST+SFLLGAFTSVVSGFLGMKIATYANARTTLEAR
Sbjct: 121 FSTKPQPCSYDKSKTCKPALATAIFSTISFLLGAFTSVVSGFLGMKIATYANARTTLEAR 180

Query: 181 KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS 240
           KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS
Sbjct: 181 KGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSS 240

Query: 241 MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF 300
           MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF
Sbjct: 241 MALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLF 300

Query: 301 GSYAESSCAALVVASISSFGNNHELTPMLYPLIISSVGILVCLITTLFATDFFEIKAVKE 360
           GSYAESSCAALVVASISSFGNNHELTPMLYPLI+SSVGILVCL+TTLFATDFFEIKAVKE
Sbjct: 301 GSYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKE 360

Query: 361 IEPSLKKQLIISTVLMTFGIAIVTWIAVPSTFTIFNFGTQKVVKNWKLFLCVAVGLWAGL 420
           IEP+LKKQLIISTVLMTFG+AIVTWI+VPSTFTIFNFGTQK V NWKLFLCVAVGLWAGL
Sbjct: 361 IEPALKKQLIISTVLMTFGVAIVTWISVPSTFTIFNFGTQKDVTNWKLFLCVAVGLWAGL 420

Query: 421 IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFSF 480
           IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSF+F
Sbjct: 421 IIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF 480

Query: 481 AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT 540
           AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT
Sbjct: 481 AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTT 540

Query: 541 AAIGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKS 600
           AAIGKGFAIGSAALVSLALFGAFVSRAGV AVDVLTPKVFIGLIVGAMLPYWFSAMTMKS
Sbjct: 541 AAIGKGFAIGSAALVSLALFGAFVSRAGVNAVDVLTPKVFIGLIVGAMLPYWFSAMTMKS 600

Query: 601 VGSAALKMVEEVRRQFNTIPGLMEGTTKPDYATCVKISTDASIKEMIPPGALVMLTPLIV 660
           VGSAALKMVEEVRRQFNTIPGLMEGT KPDYATCVKISTDASIKEMIPPGALVMLTPLIV
Sbjct: 601 VGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIV 660

Query: 661 GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH 720
           GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH
Sbjct: 661 GILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPH 720

Query: 721 KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 768
           KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI
Sbjct: 721 KAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 769

BLAST of Sgr027089 vs. ExPASy TrEMBL
Match: O82680 (H(+)-exporting diphosphatase OS=Cucurbita moschata OX=3662 GN=PHP-1 PE=2 SV=1)

HSP 1 Score: 1404.4 bits (3634), Expect = 0.0e+00
Identity = 740/767 (96.48%), Postives = 757/767 (98.70%), Query Frame = 0

Query: 1   MGVTILPDLGTQILIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNSSAGKNGYSDYL 60
           M VTILPDLGT+I IPVCA++GIVFSLVQWYYVSQVKLSPGRD+AHNNS+  KNGYSDYL
Sbjct: 1   MSVTILPDLGTEIFIPVCAVIGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYL 60

Query: 61  IEEEEGVNDHSVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVESFS 120
           IEEEEGVNDH+VVIKCAEIQ+AISEGATSFLFTEYKYVGIFMV FAVLIF+FLGSVESFS
Sbjct: 61  IEEEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFS 120

Query: 121 TKPQPCTYDKTKICKPALATAIFSTVSFLLGAFTSVVSGFLGMKIATYANARTTLEARKG 180
           TKPQPCTYDKT+ CKPALATAIFSTVSFLLGA TSVVSGFLGMKIATYANARTTLEARKG
Sbjct: 121 TKPQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKG 180

Query: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240
           VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA
Sbjct: 181 VGKAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMA 240

Query: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300
           LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS
Sbjct: 241 LFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGS 300

Query: 301 YAESSCAALVVASISSFGNNHELTPMLYPLIISSVGILVCLITTLFATDFFEIKAVKEIE 360
           YAESSCAALVVASIS FGNNHELTPMLYPLI+SS+GILVCLITTLFATDFFEIKAVKEIE
Sbjct: 301 YAESSCAALVVASISPFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIE 360

Query: 361 PSLKKQLIISTVLMTFGIAIVTWIAVPSTFTIFNFGTQKVVKNWKLFLCVAVGLWAGLII 420
           P+LKKQLIISTVLMTFGIAIVTW++VPS+FTIFNFGTQKVV NWKLFLCVAVGLWAGLII
Sbjct: 361 PALKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLII 420

Query: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFSFAA 480
           GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSF+FAA
Sbjct: 421 GFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAA 480

Query: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540
           MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA
Sbjct: 481 MYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAA 540

Query: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600
           IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG
Sbjct: 541 IGKGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVG 600

Query: 601 SAALKMVEEVRRQFNTIPGLMEGTTKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660
           SAALKMVEEVRRQFNTIPGLMEGT KPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI
Sbjct: 601 SAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGI 660

Query: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKA 720
           LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGAS+HARTLGPKGSDPHKA
Sbjct: 661 LFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASKHARTLGPKGSDPHKA 720

Query: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 768
           AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI
Sbjct: 721 AVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 767

BLAST of Sgr027089 vs. ExPASy TrEMBL
Match: A0A6J1H8E2 (H(+)-exporting diphosphatase OS=Cucurbita moschata OX=3662 GN=LOC111461438 PE=3 SV=1)

HSP 1 Score: 1402.9 bits (3630), Expect = 0.0e+00
Identity = 739/765 (96.60%), Postives = 756/765 (98.82%), Query Frame = 0

Query: 3   VTILPDLGTQILIPVCAIVGIVFSLVQWYYVSQVKLSPGRDSAHNNSSAGKNGYSDYLIE 62
           VTILPDLGTQI IPVCA+VGIVFSLVQWYYVSQVKLSP RDS HNNS+A KNGYSDYLIE
Sbjct: 4   VTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPSRDSTHNNSAAAKNGYSDYLIE 63

Query: 63  EEEGVNDHSVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVESFSTK 122
           EEEGVNDH+VVIKCAEIQNAISEGATSFLFTEYKYVGIFMV FAVLIF+FLGSVE FST+
Sbjct: 64  EEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGFSTE 123

Query: 123 PQPCTYDKTKICKPALATAIFSTVSFLLGAFTSVVSGFLGMKIATYANARTTLEARKGVG 182
           PQ CTYDKTKICKPALATAIFST+SFLLGA TSVVSGFLGMKIATYANARTTLEARKGVG
Sbjct: 124 PQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVG 183

Query: 183 KAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALF 242
           KAFITAFRSGAVMGFLLAANGLLVLFIAIN+FKLYYG+DWGGLFESITGYGLGGSSMALF
Sbjct: 184 KAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSMALF 243

Query: 243 GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA 302
           GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA
Sbjct: 244 GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA 303

Query: 303 ESSCAALVVASISSFGNNHELTPMLYPLIISSVGILVCLITTLFATDFFEIKAVKEIEPS 362
           ESSCAALVVASISSFGNNHELTPMLYPLI+SSVGILVCLITTLFATDFFEIKAVKEIEP+
Sbjct: 304 ESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEIEPA 363

Query: 363 LKKQLIISTVLMTFGIAIVTWIAVPSTFTIFNFGTQKVVKNWKLFLCVAVGLWAGLIIGF 422
           LKKQLIISTVLMTFGIA+VTW+AVPSTFTIFNFGTQKVV+NWKLFLCVAVGLWAGLIIGF
Sbjct: 364 LKKQLIISTVLMTFGIAVVTWLAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLIIGF 423

Query: 423 VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFSFAAMY 482
           VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSF+FAAMY
Sbjct: 424 VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMY 483

Query: 483 GIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG 542
           GIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG
Sbjct: 484 GIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG 543

Query: 543 KGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSA 602
           KGFAIGSAALVSLALFGAFVSRAGVT+VDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSA
Sbjct: 544 KGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSA 603

Query: 603 ALKMVEEVRRQFNTIPGLMEGTTKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILF 662
           ALKMVEEVRRQFNTIPGLMEGT KPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILF
Sbjct: 604 ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILF 663

Query: 663 GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAV 722
           GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAV
Sbjct: 664 GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAV 723

Query: 723 IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 768
           IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA+HGGLLFK+
Sbjct: 724 IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFKL 768

BLAST of Sgr027089 vs. TAIR 10
Match: AT1G15690.1 (Inorganic H pyrophosphatase family protein )

HSP 1 Score: 1280.0 bits (3311), Expect = 0.0e+00
Identity = 668/763 (87.55%), Postives = 717/763 (93.97%), Query Frame = 0

Query: 5   ILPDLGTQILIPVCAIVGIVFSLVQWYYVSQVKLSPGRD-SAHNNSSAGKNGYSDYLIEE 64
           +LP+L T+IL+P+CA++GI FSL QWY VS+VKL+     S+   ++ GKNGY DYLIEE
Sbjct: 6   LLPELWTEILVPICAVIGIAFSLFQWYVVSRVKLTSDLGASSSGGANNGKNGYGDYLIEE 65

Query: 65  EEGVNDHSVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVESFSTKP 124
           EEGVND SVV KCAEIQ AISEGATSFLFTEYKYVG+FM+ FA +IF+FLGSVE FST  
Sbjct: 66  EEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEGFSTDN 125

Query: 125 QPCTYDKTKICKPALATAIFSTVSFLLGAFTSVVSGFLGMKIATYANARTTLEARKGVGK 184
           +PCTYD T+ CKPALATA FST++F+LGA TSV+SGFLGMKIATYANARTTLEARKGVGK
Sbjct: 126 KPCTYDTTRTCKPALATAAFSTIAFVLGAVTSVLSGFLGMKIATYANARTTLEARKGVGK 185

Query: 185 AFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALFG 244
           AFI AFRSGAVMGFLLAA+GLLVL+I IN+FK+YYGDDW GLFE+ITGYGLGGSSMALFG
Sbjct: 186 AFIVAFRSGAVMGFLLAASGLLVLYITINVFKIYYGDDWEGLFEAITGYGLGGSSMALFG 245

Query: 245 RVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 304
           RVGGGIYTKAADVGADLVGK+ERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE
Sbjct: 246 RVGGGIYTKAADVGADLVGKIERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 305

Query: 305 SSCAALVVASISSFGNNHELTPMLYPLIISSVGILVCLITTLFATDFFEIKAVKEIEPSL 364
           +SCAALVVASISSFG NH+ T M YPL+ISS+GILVCLITTLFATDFFEIK VKEIEP+L
Sbjct: 306 ASCAALVVASISSFGINHDFTAMCYPLLISSMGILVCLITTLFATDFFEIKLVKEIEPAL 365

Query: 365 KKQLIISTVLMTFGIAIVTWIAVPSTFTIFNFGTQKVVKNWKLFLCVAVGLWAGLIIGFV 424
           K QLIISTV+MT GIAIV+W+ +P++FTIFNFGTQKVVKNW+LFLCV VGLWAGLIIGFV
Sbjct: 366 KNQLIISTVIMTVGIAIVSWVGLPTSFTIFNFGTQKVVKNWQLFLCVCVGLWAGLIIGFV 425

Query: 425 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFSFAAMYG 484
           TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA+SIFVSFSFAAMYG
Sbjct: 426 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFSFAAMYG 485

Query: 485 IAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 544
           +AVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK
Sbjct: 486 VAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 545

Query: 545 GFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAA 604
           GFAIGSAALVSLALFGAFVSRAG+  VDVLTPKV IGL+VGAMLPYWFSAMTMKSVGSAA
Sbjct: 546 GFAIGSAALVSLALFGAFVSRAGIHTVDVLTPKVIIGLLVGAMLPYWFSAMTMKSVGSAA 605

Query: 605 LKMVEEVRRQFNTIPGLMEGTTKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFG 664
           LKMVEEVRRQFNTIPGLMEGT KPDYATCVKISTDASIKEMIPPG LVMLTPLIVG  FG
Sbjct: 606 LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGCLVMLTPLIVGFFFG 665

Query: 665 VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVI 724
           VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAG SEHA++LGPKGS+PHKAAVI
Sbjct: 666 VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGVSEHAKSLGPKGSEPHKAAVI 725

Query: 725 GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFK 767
           GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA+HGG+LFK
Sbjct: 726 GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGILFK 768

BLAST of Sgr027089 vs. TAIR 10
Match: AT1G15690.2 (Inorganic H pyrophosphatase family protein )

HSP 1 Score: 1038.1 bits (2683), Expect = 3.5e-303
Identity = 542/628 (86.31%), Postives = 586/628 (93.31%), Query Frame = 0

Query: 5   ILPDLGTQILIPVCAIVGIVFSLVQWYYVSQVKLSPGRD-SAHNNSSAGKNGYSDYLIEE 64
           +LP+L T+IL+P+CA++GI FSL QWY VS+VKL+     S+   ++ GKNGY DYLIEE
Sbjct: 6   LLPELWTEILVPICAVIGIAFSLFQWYVVSRVKLTSDLGASSSGGANNGKNGYGDYLIEE 65

Query: 65  EEGVNDHSVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAVLIFLFLGSVESFSTKP 124
           EEGVND SVV KCAEIQ AISEGATSFLFTEYKYVG+FM+ FA +IF+FLGSVE FST  
Sbjct: 66  EEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEGFSTDN 125

Query: 125 QPCTYDKTKICKPALATAIFSTVSFLLGAFTSVVSGFLGMKIATYANARTTLEARKGVGK 184
           +PCTYD T+ CKPALATA FST++F+LGA TSV+SGFLGMKIATYANARTTLEARKGVGK
Sbjct: 126 KPCTYDTTRTCKPALATAAFSTIAFVLGAVTSVLSGFLGMKIATYANARTTLEARKGVGK 185

Query: 185 AFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALFG 244
           AFI AFRSGAVMGFLLAA+GLLVL+I IN+FK+YYGDDW GLFE+ITGYGLGGSSMALFG
Sbjct: 186 AFIVAFRSGAVMGFLLAASGLLVLYITINVFKIYYGDDWEGLFEAITGYGLGGSSMALFG 245

Query: 245 RVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 304
           RVGGGIYTKAADVGADLVGK+ERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE
Sbjct: 246 RVGGGIYTKAADVGADLVGKIERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 305

Query: 305 SSCAALVVASISSFGNNHELTPMLYPLIISSVGILVCLITTLFATDFFEIKAVKEIEPSL 364
           +SCAALVVASISSFG NH+ T M YPL+ISS+GILVCLITTLFATDFFEIK VKEIEP+L
Sbjct: 306 ASCAALVVASISSFGINHDFTAMCYPLLISSMGILVCLITTLFATDFFEIKLVKEIEPAL 365

Query: 365 KKQLIISTVLMTFGIAIVTWIAVPSTFTIFNFGTQKVVKNWKLFLCVAVGLWAGLIIGFV 424
           K QLIISTV+MT GIAIV+W+ +P++FTIFNFGTQKVVKNW+LFLCV VGLWAGLIIGFV
Sbjct: 366 KNQLIISTVIMTVGIAIVSWVGLPTSFTIFNFGTQKVVKNWQLFLCVCVGLWAGLIIGFV 425

Query: 425 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFSFAAMYG 484
           TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA+SIFVSFSFAAMYG
Sbjct: 426 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFSFAAMYG 485

Query: 485 IAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 544
           +AVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK
Sbjct: 486 VAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 545

Query: 545 GFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAA 604
           GFAIGSAALVSLALFGAFVSRAG+  VDVLTPKV IGL+VGAMLPYWFSAMTMKSVGSAA
Sbjct: 546 GFAIGSAALVSLALFGAFVSRAGIHTVDVLTPKVIIGLLVGAMLPYWFSAMTMKSVGSAA 605

Query: 605 LKMVEEVRRQFNTIPGLMEGTTKPDYAT 632
           LKMVEEVRRQFNTIPGLMEGT KPDYAT
Sbjct: 606 LKMVEEVRRQFNTIPGLMEGTAKPDYAT 633

BLAST of Sgr027089 vs. TAIR 10
Match: AT1G16780.1 (Inorganic H pyrophosphatase family protein )

HSP 1 Score: 424.5 bits (1090), Expect = 1.8e-118
Identity = 291/735 (39.59%), Postives = 417/735 (56.73%), Query Frame = 0

Query: 78  EIQNAISEGATSFLFTEYKYVG--IFMVAFAVL-IFLFLGSVESFSTKPQPCTYDKTKIC 137
           +I +AI +GA  FL T+Y  +    F++AF +L I+LF     + + + +     +T   
Sbjct: 150 QISDAIRDGAEGFLRTQYGTISKMAFLLAFVILCIYLF----RNLTPQQEASGLGRT--- 209

Query: 138 KPALATAIFSTVSFLLGAFTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAV 197
                +A  +  +FLLGA  S ++G++GM ++  AN R +  AR+   +A   A R+G  
Sbjct: 210 ----MSAYITVAAFLLGALCSGIAGYVGMWVSVRANVRVSSAARRSAREALQIAVRAGGF 269

Query: 198 MGFLLAANGLLVLFIAINLFKLYYGDDWGGLFE------SITGYGLGGSSMALFGRVGGG 257
              ++    ++ + I  + F ++   D  G  +       + GYG G S +ALF ++GGG
Sbjct: 270 SALVVVGMAVIGIAILYSTFYVWLDVDSPGSMKVTDLPLLLVGYGFGASFVALFAQLGGG 329

Query: 258 IYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAA 317
           IYTK ADVGADLVGKVE  IPEDDPRNPAVIAD VGDNVGD A  G+DLF S A    +A
Sbjct: 330 IYTKGADVGADLVGKVEHGIPEDDPRNPAVIADLVGDNVGDCAARGADLFESIAAEIISA 389

Query: 318 LVVASISSFGNNHE------LTPML---YPLIISSVGILVCLITTLFATDFFEIKAVKEI 377
           +++    +     E      L P++   + L+ISS+GIL     ++  T    +K+  E 
Sbjct: 390 MILGGTMAQKCKIEDPSGFILFPLVVHSFDLVISSIGIL-----SIKGTRNASVKSPVE- 449

Query: 378 EPSLKKQ----LIISTVLMTFGIAIVTWIAVPSTFTIFNFGTQKVVKNW-KLFLCVAVGL 437
           +P +  Q    L I   ++TFG A   W+            T++    W   F+C  VG+
Sbjct: 450 DPMVVLQKGYSLTIILAVLTFG-ASTRWLLY----------TEQAPSAWLNFFMCGLVGI 509

Query: 438 WAGLIIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFV 497
               +  +++ YYT   Y PV+ +A +  TG  TN+I G++LG +S  +P+  I+V+I  
Sbjct: 510 ITAYVFVWISRYYTDYKYEPVRTLALASSTGHGTNIIAGVSLGLESTALPVLVISVAIIS 569

Query: 498 SF--------------SFAAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGM 557
           +F                  ++G AVA +GMLST A  L +D +GPI+DNAGGI EM+  
Sbjct: 570 AFWLGNTSGLIDEKGNPTGGLFGTAVATMGMLSTAAYVLTMDMFGPIADNAGGIVEMSQQ 629

Query: 558 SHRIRERTDALDAAGNTTAAIGKGFAIGSAALVSLALFGAFVSRAGVTA------VDVLT 617
              +RE TD LDA GNTT A  KGFAIGSAAL S  LF A++      A      VD+  
Sbjct: 630 PESVREITDVLDAVGNTTKATTKGFAIGSAALASFLLFSAYMDEVSAFANVSFKEVDIAI 689

Query: 618 PKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEEVRRQFNTIPGLMEGTTKPDYATCVK 677
           P+VFIG ++GAML + FSA    +VG  A ++V EVRRQF   PG+M+   KPDY  CV 
Sbjct: 690 PEVFIGGLLGAMLIFLFSAWACAAVGRTAQEVVNEVRRQFIERPGIMDYKEKPDYGRCVA 749

Query: 678 ISTDASIKEMIPPGALVMLTPLIVGILF------------GVETLSGVLAGSLVSGVQIA 737
           I   ++++EMI PGAL +++P+ VG +F            G + ++ +L  + V G+ +A
Sbjct: 750 IVASSALREMIKPGALAIISPIAVGFVFRILGYYTGQPLLGAKVVAAMLMFATVCGILMA 809

Query: 738 ISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPLKDTSGPSLNIL 758
           +  +  GGAWDNAKKYIE GA      LG KGSD HKAAV GDT+GDP KDT+GPS+++L
Sbjct: 810 LFLNTAGGAWDNAKKYIETGA------LGGKGSDSHKAAVTGDTVGDPFKDTAGPSIHVL 850

BLAST of Sgr027089 vs. TAIR 10
Match: AT1G78920.1 (vacuolar H+-pyrophosphatase 2 )

HSP 1 Score: 417.9 bits (1073), Expect = 1.7e-116
Identity = 279/724 (38.54%), Postives = 410/724 (56.63%), Query Frame = 0

Query: 78  EIQNAISEGATSFLFTEYKYVG--IFMVAFAVL-IFLFLGSVESFSTKPQPCTYDKTKIC 137
           EI +AI +GA  F  T+Y  +     ++AF +L I+LF     S + + +     +    
Sbjct: 101 EISDAIRDGAEGFFRTQYSTISKMAILLAFVILCIYLF----RSLTPQQEAAGLGRA--- 160

Query: 138 KPALATAIFSTVSFLLGAFTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAV 197
                +A  +  +FLLGA  S ++G++GM ++  AN R +  AR+   +A   A R+G  
Sbjct: 161 ----MSAYITVAAFLLGALCSGIAGYVGMWVSVRANVRVSSAARRSAREALQIAVRAGGF 220

Query: 198 MGFLLAANGLLVLFIAINLFKLYYGDDWGGLFE------SITGYGLGGSSMALFGRVGGG 257
              ++    ++ + I  + F ++ G    G          + GYG G S +ALF ++GGG
Sbjct: 221 SALVVVGMAVIGIAILYSTFYVWLGVGSPGSMNVTDLPLLLVGYGFGASFVALFAQLGGG 280

Query: 258 IYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAA 317
           IYTK ADVGADLVGKVE+ IPEDDPRNPAVIAD VGDNVGD A  G+DLF S A    +A
Sbjct: 281 IYTKGADVGADLVGKVEQGIPEDDPRNPAVIADLVGDNVGDCAARGADLFESIAAEIISA 340

Query: 318 LVVASISSFGNNHELTP--MLYPLIISSVGILVCLITTLFATDFFEIKAVKEIEPSLKKQ 377
           +++    +     E     +L+PL++ S  +++  I  L       IK  ++       +
Sbjct: 341 MILGGTMAKKCKIEDPSGFILFPLVVHSFDLIISSIGIL------SIKGTRDASVKSPVE 400

Query: 378 LIISTVLMTFGIAIVTWIAVPSTFTIFNFGTQKVVKNWKLF-LCVAVGLWAGLIIGFVTE 437
             ++ +   + + I+  +      T +   T++    W  F LC  VG+    I  ++++
Sbjct: 401 DPMAVLQKGYSLTIILAVITFGASTRWLLYTEQAPSAWFNFALCGLVGIITAYIFVWISK 460

Query: 438 YYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSF--------- 497
           YYT   + PV+ +A +  TG  TN+I G++LG +S  +P+  I+V+I  ++         
Sbjct: 461 YYTDYKHEPVRTLALASSTGHGTNIIAGVSLGLESTALPVLTISVAIISAYWLGNTSGLV 520

Query: 498 -----SFAAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDAL 557
                    ++G AVA +GMLST A  L +D +GPI+DNAGGI EM+     +RE TD L
Sbjct: 521 DENGIPTGGLFGTAVATMGMLSTAAYVLTMDMFGPIADNAGGIVEMSQQPESVREITDLL 580

Query: 558 DAAGNTTAAIGKGFAIGSAALVSLALFGAFVSRAGVTA------VDVLTPKVFIGLIVGA 617
           DA GNTT A  KGFAIGSAAL S  LF A++      A      VD+  P+VF+G ++GA
Sbjct: 581 DAVGNTTKATTKGFAIGSAALASFLLFSAYMDEVSAFANVSFKEVDIAIPEVFVGGLLGA 640

Query: 618 MLPYWFSAMTMKSVGSAALKMVEEVRRQFNTIPGLMEGTTKPDYATCVKISTDASIKEMI 677
           ML + FSA    +VG  A ++V EVRRQF   PG+ME   KPDY+ CV I   A+++EMI
Sbjct: 641 MLIFLFSAWACAAVGRTAQEVVNEVRRQFIERPGIMEYKEKPDYSRCVAIVASAALREMI 700

Query: 678 PPGALVMLTPLIVGILF------------GVETLSGVLAGSLVSGVQIAISASNTGGAWD 737
            PGAL + +P++VG++F            G + ++ +L  + V G+ +A+  +  GGAWD
Sbjct: 701 KPGALAIASPIVVGLVFRILGYYTGQPLLGAKVVASMLMFATVCGILMALFLNTAGGAWD 760

Query: 738 NAKKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVF 758
           NAKKYIE GA      LG KGS+ HKAAV GDT+GDP KDT+GPS+++LIK++A  +LV 
Sbjct: 761 NAKKYIETGA------LGGKGSEAHKAAVTGDTVGDPFKDTAGPSIHVLIKMLATITLVM 801

BLAST of Sgr027089 vs. TAIR 10
Match: AT1G78920.2 (vacuolar H+-pyrophosphatase 2 )

HSP 1 Score: 417.9 bits (1073), Expect = 1.7e-116
Identity = 279/724 (38.54%), Postives = 410/724 (56.63%), Query Frame = 0

Query: 78  EIQNAISEGATSFLFTEYKYVG--IFMVAFAVL-IFLFLGSVESFSTKPQPCTYDKTKIC 137
           EI +AI +GA  F  T+Y  +     ++AF +L I+LF     S + + +     +    
Sbjct: 101 EISDAIRDGAEGFFRTQYSTISKMAILLAFVILCIYLF----RSLTPQQEAAGLGRA--- 160

Query: 138 KPALATAIFSTVSFLLGAFTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAV 197
                +A  +  +FLLGA  S ++G++GM ++  AN R +  AR+   +A   A R+G  
Sbjct: 161 ----MSAYITVAAFLLGALCSGIAGYVGMWVSVRANVRVSSAARRSAREALQIAVRAGGF 220

Query: 198 MGFLLAANGLLVLFIAINLFKLYYGDDWGGLFE------SITGYGLGGSSMALFGRVGGG 257
              ++    ++ + I  + F ++ G    G          + GYG G S +ALF ++GGG
Sbjct: 221 SALVVVGMAVIGIAILYSTFYVWLGVGSPGSMNVTDLPLLLVGYGFGASFVALFAQLGGG 280

Query: 258 IYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAA 317
           IYTK ADVGADLVGKVE+ IPEDDPRNPAVIAD VGDNVGD A  G+DLF S A    +A
Sbjct: 281 IYTKGADVGADLVGKVEQGIPEDDPRNPAVIADLVGDNVGDCAARGADLFESIAAEIISA 340

Query: 318 LVVASISSFGNNHELTP--MLYPLIISSVGILVCLITTLFATDFFEIKAVKEIEPSLKKQ 377
           +++    +     E     +L+PL++ S  +++  I  L       IK  ++       +
Sbjct: 341 MILGGTMAKKCKIEDPSGFILFPLVVHSFDLIISSIGIL------SIKGTRDASVKSPVE 400

Query: 378 LIISTVLMTFGIAIVTWIAVPSTFTIFNFGTQKVVKNWKLF-LCVAVGLWAGLIIGFVTE 437
             ++ +   + + I+  +      T +   T++    W  F LC  VG+    I  ++++
Sbjct: 401 DPMAVLQKGYSLTIILAVITFGASTRWLLYTEQAPSAWFNFALCGLVGIITAYIFVWISK 460

Query: 438 YYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSF--------- 497
           YYT   + PV+ +A +  TG  TN+I G++LG +S  +P+  I+V+I  ++         
Sbjct: 461 YYTDYKHEPVRTLALASSTGHGTNIIAGVSLGLESTALPVLTISVAIISAYWLGNTSGLV 520

Query: 498 -----SFAAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDAL 557
                    ++G AVA +GMLST A  L +D +GPI+DNAGGI EM+     +RE TD L
Sbjct: 521 DENGIPTGGLFGTAVATMGMLSTAAYVLTMDMFGPIADNAGGIVEMSQQPESVREITDLL 580

Query: 558 DAAGNTTAAIGKGFAIGSAALVSLALFGAFVSRAGVTA------VDVLTPKVFIGLIVGA 617
           DA GNTT A  KGFAIGSAAL S  LF A++      A      VD+  P+VF+G ++GA
Sbjct: 581 DAVGNTTKATTKGFAIGSAALASFLLFSAYMDEVSAFANVSFKEVDIAIPEVFVGGLLGA 640

Query: 618 MLPYWFSAMTMKSVGSAALKMVEEVRRQFNTIPGLMEGTTKPDYATCVKISTDASIKEMI 677
           ML + FSA    +VG  A ++V EVRRQF   PG+ME   KPDY+ CV I   A+++EMI
Sbjct: 641 MLIFLFSAWACAAVGRTAQEVVNEVRRQFIERPGIMEYKEKPDYSRCVAIVASAALREMI 700

Query: 678 PPGALVMLTPLIVGILF------------GVETLSGVLAGSLVSGVQIAISASNTGGAWD 737
            PGAL + +P++VG++F            G + ++ +L  + V G+ +A+  +  GGAWD
Sbjct: 701 KPGALAIASPIVVGLVFRILGYYTGQPLLGAKVVASMLMFATVCGILMALFLNTAGGAWD 760

Query: 738 NAKKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVF 758
           NAKKYIE GA      LG KGS+ HKAAV GDT+GDP KDT+GPS+++LIK++A  +LV 
Sbjct: 761 NAKKYIETGA------LGGKGSEAHKAAVTGDTVGDPFKDTAGPSIHVLIKMLATITLVM 801

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022929683.10.0e+0096.87pyrophosphate-energized vacuolar membrane proton pump-like [Cucurbita moschata] ... [more]
XP_023002108.10.0e+0096.61pyrophosphate-energized vacuolar membrane proton pump-like [Cucurbita maxima][more]
XP_023529940.10.0e+0097.00pyrophosphate-energized vacuolar membrane proton pump-like [Cucurbita pepo subsp... [more]
XP_022135071.10.0e+0096.62pyrophosphate-energized vacuolar membrane proton pump [Momordica charantia][more]
XP_023515840.10.0e+0096.73pyrophosphate-energized vacuolar membrane proton pump [Cucurbita pepo subsp. pep... [more]
Match NameE-valueIdentityDescription
P216160.0e+0091.53Pyrophosphate-energized vacuolar membrane proton pump OS=Vigna radiata var. radi... [more]
P314140.0e+0087.55Pyrophosphate-energized vacuolar membrane proton pump 1 OS=Arabidopsis thaliana ... [more]
Q065720.0e+0087.04Pyrophosphate-energized vacuolar membrane proton pump OS=Hordeum vulgare OX=4513... [more]
Q72Q291.3e-19856.75Putative K(+)-stimulated pyrophosphate-energized sodium pump OS=Leptospira inter... [more]
Q8F6411.3e-19856.75Putative K(+)-stimulated pyrophosphate-energized sodium pump OS=Leptospira inter... [more]
Match NameE-valueIdentityDescription
A0A6J1ESX20.0e+0096.87H(+)-exporting diphosphatase OS=Cucurbita moschata OX=3662 GN=LOC111436191 PE=3 ... [more]
A0A6J1KSL00.0e+0096.61H(+)-exporting diphosphatase OS=Cucurbita maxima OX=3661 GN=LOC111496068 PE=3 SV... [more]
A0A6J1BZK80.0e+0096.62H(+)-exporting diphosphatase OS=Momordica charantia OX=3673 GN=LOC111007149 PE=3... [more]
O826800.0e+0096.48H(+)-exporting diphosphatase OS=Cucurbita moschata OX=3662 GN=PHP-1 PE=2 SV=1[more]
A0A6J1H8E20.0e+0096.60H(+)-exporting diphosphatase OS=Cucurbita moschata OX=3662 GN=LOC111461438 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT1G15690.10.0e+0087.55Inorganic H pyrophosphatase family protein [more]
AT1G15690.23.5e-30386.31Inorganic H pyrophosphatase family protein [more]
AT1G16780.11.8e-11839.59Inorganic H pyrophosphatase family protein [more]
AT1G78920.11.7e-11638.54vacuolar H+-pyrophosphatase 2 [more]
AT1G78920.21.7e-11638.54vacuolar H+-pyrophosphatase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004131Pyrophosphate-energised proton pumpPFAMPF03030H_PPasecoord: 23..753
e-value: 8.6E-257
score: 854.0
IPR004131Pyrophosphate-energised proton pumpTIGRFAMTIGR01104TIGR01104coord: 11..763
e-value: 0.0
score: 1228.1
IPR004131Pyrophosphate-energised proton pumpPIRSFPIRSF001265H+-PPtasecoord: 10..766
e-value: 4.6E-282
score: 935.7
IPR004131Pyrophosphate-energised proton pumpPANTHERPTHR31998K(+)-INSENSITIVE PYROPHOSPHATE-ENERGIZED PROTON PUMPcoord: 1..767
IPR004131Pyrophosphate-energised proton pumpHAMAPMF_01129PPase_energized_pumpcoord: 9..758
score: 23.03438
NoneNo IPR availablePANTHERPTHR31998:SF38PYROPHOSPHATE-ENERGIZED VACUOLAR MEMBRANE PROTON PUMPcoord: 1..767

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr027089.1Sgr027089.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902600 proton transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0004427 inorganic diphosphatase activity
molecular_function GO:0009678 pyrophosphate hydrolysis-driven proton transmembrane transporter activity