Sgr026689 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr026689
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationtig00153033: 2635749 .. 2637114 (+)
RNA-Seq ExpressionSgr026689
SyntenySgr026689
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGAAGATCAAGTTGAAGTTGATACTCCTCCCTTCTTTCTCTGTCCCATCTCTCTGCAAATCATGAAAGATCCCGTCACGGTGGCCTCCGGCATAACCTACGACCGAGACAGCATCGAGAAGTGGCTGTTTTCCGATAAACATACCACGTGCCCGGTCACCAAGGCCGTCTTGTCGAACTCCGATCTCATTCCCAACCATACTCTCCGGCGACTTATTCAAGCTTGGTGCACTGTCAACGCTTGCAGCGGCGTCGAGAGGCTTCCGACTCCTAAGCCTCCGGTCGACCGAACGCAGATCGTTAAGATTCTTCAGGACGTAAAGTTATCTCCGTTCTCTCAGCAGAAATGCCTCCGGCGGCTCCGGTCCATCGCGTCGGCGAATGAAACCAACAAACGCTGCATGGAAGCTGCCGGAGTTGTGGAGTTTTTGGCCGGAGTTGTGTGTAATTCCTTTGACGCAGAATCGAGCTTCTTCGAAGGTACGGTGGTTATCATTAAGCTACCACTAAATTCAAAAGATCATCAAGCTAATGGATTTTTCATAAATTTAATCTTTTATGCGTATTTTTAACGCAGATTATGCTTTTGACGGCCATTTAGCAAATTCCGACGATGAGGCTTTGAGCATCCTTTACAAACTCCAAATCTCCGAAGCCGGCCTGAAGTTTTTACTCGGCAACAATGGCGGCGTCTTCGTCACGTCCTTAACCAAAGTGCTGCAAAACGGAAGCTACGGCTCTCGAGCTTACGCCGTAATGCTTTTGAAATCTATGTTCGAAGTCGCCGATCAGATTAAGATAATTAGCGTAAACACCGAATTCTTCATCGAAATCGTTCAAGTTCTGAAGGATCAGATCTCGCAGAAGGCCTCGAAAGCTGCATTGAAACTGCTGATCAGTCTCTGTTCGAGTTTCAGAAACAGAGTGAAGGCAGTTCAAGCCGGCGCGGTTCAGGTTCTGATCGATCTCCTCCTCGATCTCGACTCGTCGGAGAAGCGGGCGTGCGAGATGACTCTGGTGGCGCTGGACTTGCTCTGCAGTTGCGCCGACGGGCGGGCGGAGCTGCTGAACCATGCGGCGGGGCTCGCCGTCGTGTCGAAGAAGATTCTGAGAGTTTCTTGGCTGGGAAGCGAGAAGGCCGTCGGAATCCTGTGCTCCGTCGCGAAGTTCTCGGCGAGCCAAAGCGTCGTCCAGGAAATGCTGCAGCTGGGAGTGGTGACGAAGCTTTGCTTTCTGCTTCAGGTCGTCGGCGCCGGAAGCAAAGCCAAAGAGAAAGCGAAAGAGATTCTGAAGCTCCATGGTCGAACATGGCGGAATTCTTCATGCTTACTTTCAAATCTGCGATCTTCTTATCCACTTTAG

mRNA sequence

ATGGCGGAAGATCAAGTTGAAGTTGATACTCCTCCCTTCTTTCTCTGTCCCATCTCTCTGCAAATCATGAAAGATCCCGTCACGGTGGCCTCCGGCATAACCTACGACCGAGACAGCATCGAGAAGTGGCTGTTTTCCGATAAACATACCACGTGCCCGGTCACCAAGGCCGTCTTGTCGAACTCCGATCTCATTCCCAACCATACTCTCCGGCGACTTATTCAAGCTTGGTGCACTGTCAACGCTTGCAGCGGCGTCGAGAGGCTTCCGACTCCTAAGCCTCCGGTCGACCGAACGCAGATCGTTAAGATTCTTCAGGACGTAAAGTTATCTCCGTTCTCTCAGCAGAAATGCCTCCGGCGGCTCCGGTCCATCGCGTCGGCGAATGAAACCAACAAACGCTGCATGGAAGCTGCCGGAGTTGTGGAGTTTTTGGCCGGAGTTGTGTGTAATTCCTTTGACGCAGAATCGAGCTTCTTCGAAGCAAATTCCGACGATGAGGCTTTGAGCATCCTTTACAAACTCCAAATCTCCGAAGCCGGCCTGAAGTTTTTACTCGGCAACAATGGCGGCGTCTTCGTCACGTCCTTAACCAAAGTGCTGCAAAACGGAAGCTACGGCTCTCGAGCTTACGCCGTAATGCTTTTGAAATCTATGTTCGAAGTCGCCGATCAGATTAAGATAATTAGCGTAAACACCGAATTCTTCATCGAAATCGTTCAAGTTCTGAAGGATCAGATCTCGCAGAAGGCCTCGAAAGCTGCATTGAAACTGCTGATCAGTCTCTGTTCGAGTTTCAGAAACAGAGTGAAGGCAGTTCAAGCCGGCGCGGTTCAGGTTCTGATCGATCTCCTCCTCGATCTCGACTCGTCGGAGAAGCGGGCGTGCGAGATGACTCTGGTGGCGCTGGACTTGCTCTGCAGTTGCGCCGACGGGCGGGCGGAGCTGCTGAACCATGCGGCGGGGCTCGCCGTCGTGTCGAAGAAGATTCTGAGAGTTTCTTGGCTGGGAAGCGAGAAGGCCGTCGGAATCCTGTGCTCCGTCGCGAAGTTCTCGGCGAGCCAAAGCGTCGTCCAGGAAATGCTGCAGCTGGGAGTGGTGACGAAGCTTTGCTTTCTGCTTCAGGTCGTCGGCGCCGGAAGCAAAGCCAAAGAGAAAGCGAAAGAGATTCTGAAGCTCCATGGTCGAACATGGCGGAATTCTTCATGCTTACTTTCAAATCTGCGATCTTCTTATCCACTTTAG

Coding sequence (CDS)

ATGGCGGAAGATCAAGTTGAAGTTGATACTCCTCCCTTCTTTCTCTGTCCCATCTCTCTGCAAATCATGAAAGATCCCGTCACGGTGGCCTCCGGCATAACCTACGACCGAGACAGCATCGAGAAGTGGCTGTTTTCCGATAAACATACCACGTGCCCGGTCACCAAGGCCGTCTTGTCGAACTCCGATCTCATTCCCAACCATACTCTCCGGCGACTTATTCAAGCTTGGTGCACTGTCAACGCTTGCAGCGGCGTCGAGAGGCTTCCGACTCCTAAGCCTCCGGTCGACCGAACGCAGATCGTTAAGATTCTTCAGGACGTAAAGTTATCTCCGTTCTCTCAGCAGAAATGCCTCCGGCGGCTCCGGTCCATCGCGTCGGCGAATGAAACCAACAAACGCTGCATGGAAGCTGCCGGAGTTGTGGAGTTTTTGGCCGGAGTTGTGTGTAATTCCTTTGACGCAGAATCGAGCTTCTTCGAAGCAAATTCCGACGATGAGGCTTTGAGCATCCTTTACAAACTCCAAATCTCCGAAGCCGGCCTGAAGTTTTTACTCGGCAACAATGGCGGCGTCTTCGTCACGTCCTTAACCAAAGTGCTGCAAAACGGAAGCTACGGCTCTCGAGCTTACGCCGTAATGCTTTTGAAATCTATGTTCGAAGTCGCCGATCAGATTAAGATAATTAGCGTAAACACCGAATTCTTCATCGAAATCGTTCAAGTTCTGAAGGATCAGATCTCGCAGAAGGCCTCGAAAGCTGCATTGAAACTGCTGATCAGTCTCTGTTCGAGTTTCAGAAACAGAGTGAAGGCAGTTCAAGCCGGCGCGGTTCAGGTTCTGATCGATCTCCTCCTCGATCTCGACTCGTCGGAGAAGCGGGCGTGCGAGATGACTCTGGTGGCGCTGGACTTGCTCTGCAGTTGCGCCGACGGGCGGGCGGAGCTGCTGAACCATGCGGCGGGGCTCGCCGTCGTGTCGAAGAAGATTCTGAGAGTTTCTTGGCTGGGAAGCGAGAAGGCCGTCGGAATCCTGTGCTCCGTCGCGAAGTTCTCGGCGAGCCAAAGCGTCGTCCAGGAAATGCTGCAGCTGGGAGTGGTGACGAAGCTTTGCTTTCTGCTTCAGGTCGTCGGCGCCGGAAGCAAAGCCAAAGAGAAAGCGAAAGAGATTCTGAAGCTCCATGGTCGAACATGGCGGAATTCTTCATGCTTACTTTCAAATCTGCGATCTTCTTATCCACTTTAG

Protein sequence

MAEDQVEVDTPPFFLCPISLQIMKDPVTVASGITYDRDSIEKWLFSDKHTTCPVTKAVLSNSDLIPNHTLRRLIQAWCTVNACSGVERLPTPKPPVDRTQIVKILQDVKLSPFSQQKCLRRLRSIASANETNKRCMEAAGVVEFLAGVVCNSFDAESSFFEANSDDEALSILYKLQISEAGLKFLLGNNGGVFVTSLTKVLQNGSYGSRAYAVMLLKSMFEVADQIKIISVNTEFFIEIVQVLKDQISQKASKAALKLLISLCSSFRNRVKAVQAGAVQVLIDLLLDLDSSEKRACEMTLVALDLLCSCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKFSASQSVVQEMLQLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLLSNLRSSYPL
Homology
BLAST of Sgr026689 vs. NCBI nr
Match: XP_022978192.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima])

HSP 1 Score: 656.0 bits (1691), Expect = 2.2e-184
Identity = 333/422 (78.91%), Postives = 380/422 (90.05%), Query Frame = 0

Query: 1   MAEDQVEVDTPPFFLCPISLQIMKDPVTVASGITYDRDSIEKWLFSDKHTTCPVTKAVLS 60
           MAE   E+D PPFFLCPISLQIMKDPVTVASGITYDR+SIE+WLFSD+H TCPVT+ VLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLS 60

Query: 61  NSDLIPNHTLRRLIQAWCTVNACSGVERLPTPKPPVDRTQIVKILQDVKLSPFSQQKCLR 120
           + D+ PNHT+RR+IQAWCT+NAC G+ER+PTPKPP+D  Q+V+IL D KLSPFSQQ CLR
Sbjct: 61  SFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLR 120

Query: 121 RLRSIASANETNKRCMEAAGVVEFLAGVVCNSFDAESSFFE---------ANSDDEALSI 180
           RLRSIA+A+E+NKRCMEAAG VEFLAG+VCNS + E S  +         AN  DEALSI
Sbjct: 121 RLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEALSI 180

Query: 181 LYKLQISEAGLKFLLGNNGGVFVTSLTKVLQNGSYGSRAYAVMLLKSMFEVADQIKIISV 240
           LYKLQISE+GLKFLL NNGG+FV++LTK+LQNG+YGSRAY+VMLL SMFEVADQI+I+S+
Sbjct: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240

Query: 241 NTEFFIEIVQVLKDQISQKASKAALKLLISLCSSFRNRVKAVQAGAVQVLIDLLLDLDSS 300
           NT+FFIEIVQ+LKDQIS++ASK+ALKLLI LCSS RNRVKAVQAGA+ VLIDLLLDLDSS
Sbjct: 241 NTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLDSS 300

Query: 301 EKRACEMTLVALDLLCSCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKF 360
           EKR+CEM LV LDLLC CADGRAE+LNHAAG+AVVSKKILRVS LGSEKAVGILCS+A+F
Sbjct: 301 EKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF 360

Query: 361 SASQSVVQEMLQLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLLSNLRSS 414
           SAS+SVVQEM++LGVVTKLCFLLQVVGAG KAKEKAKEILKLHGRTWRNSSCL S+LRSS
Sbjct: 361 SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420

BLAST of Sgr026689 vs. NCBI nr
Match: XP_023543358.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 654.4 bits (1687), Expect = 6.3e-184
Identity = 334/422 (79.15%), Postives = 379/422 (89.81%), Query Frame = 0

Query: 1   MAEDQVEVDTPPFFLCPISLQIMKDPVTVASGITYDRDSIEKWLFSDKHTTCPVTKAVLS 60
           MAE   E+D PPFFLCPISLQIMKDPVTVASGITYDR+SIEKWLFSD+H TCPVT+ VLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVLS 60

Query: 61  NSDLIPNHTLRRLIQAWCTVNACSGVERLPTPKPPVDRTQIVKILQDVKLSPFSQQKCLR 120
           + D+ PNHTLRR+IQAWCTVNAC G+ER+PTPKPPVD  Q+V+IL D KLSPFSQQ CLR
Sbjct: 61  SFDITPNHTLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLR 120

Query: 121 RLRSIASANETNKRCMEAAGVVEFLAGVVCNSFDAESSFFE---------ANSDDEALSI 180
           RLRSIA+ +E+NKRCMEAAG VEFLAG+VCNS + E S  +         AN  DEALSI
Sbjct: 121 RLRSIAAGSESNKRCMEAAGAVEFLAGIVCNSTNVEFSSEDYAYLIDDQFANLGDEALSI 180

Query: 181 LYKLQISEAGLKFLLGNNGGVFVTSLTKVLQNGSYGSRAYAVMLLKSMFEVADQIKIISV 240
           LYKLQISE+GLKFLL NNGG+FV++LTK+LQNG+YGSRAY+VMLL SMFEVADQI+I+S+
Sbjct: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240

Query: 241 NTEFFIEIVQVLKDQISQKASKAALKLLISLCSSFRNRVKAVQAGAVQVLIDLLLDLDSS 300
           NT+FF+EIVQ+LKDQIS++ASK+ALKLLI LCSS RNRVKAV+AGAV V+IDLLLDLDSS
Sbjct: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLDLDSS 300

Query: 301 EKRACEMTLVALDLLCSCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKF 360
           EKR+CEM LV LDLLC CADGRAE+LNHAAG+AVVSKKILRVS LGSEKAVGILCS+A+F
Sbjct: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF 360

Query: 361 SASQSVVQEMLQLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLLSNLRSS 414
           SAS+SVVQEM++LGVVTKLCFLLQVVGAG KAKEKAKEILKLHGRTWRNSSCL S+LRSS
Sbjct: 361 SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420

BLAST of Sgr026689 vs. NCBI nr
Match: XP_022950790.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita moschata])

HSP 1 Score: 646.7 bits (1667), Expect = 1.3e-181
Identity = 330/422 (78.20%), Postives = 376/422 (89.10%), Query Frame = 0

Query: 1   MAEDQVEVDTPPFFLCPISLQIMKDPVTVASGITYDRDSIEKWLFSDKHTTCPVTKAVLS 60
           MAE   E+D PPFFLCPISLQIMKDPVTVASGITYDR+SIE+WLFSD+H TCP+T+ VLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLS 60

Query: 61  NSDLIPNHTLRRLIQAWCTVNACSGVERLPTPKPPVDRTQIVKILQDVKLSPFSQQKCLR 120
           N D+ PNHTLRR+IQAWCTVNAC GVER+PTPKPPVD  Q+V+IL D KLS FSQQ CLR
Sbjct: 61  NFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCLR 120

Query: 121 RLRSIASANETNKRCMEAAGVVEFLAGVVCNSFDAESSFFE---------ANSDDEALSI 180
           RL+SIA+A+E+NKRCMEAAG VEFLAG+VCNS + E S  +         AN   EALSI
Sbjct: 121 RLQSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFEALSI 180

Query: 181 LYKLQISEAGLKFLLGNNGGVFVTSLTKVLQNGSYGSRAYAVMLLKSMFEVADQIKIISV 240
           LYKLQISE+GLKFLL NNGG+FV++LTK+LQNG+YGSR Y+VMLL SMFEVADQI+I+S+
Sbjct: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQILSL 240

Query: 241 NTEFFIEIVQVLKDQISQKASKAALKLLISLCSSFRNRVKAVQAGAVQVLIDLLLDLDSS 300
           NT+FF+EIVQ+LKDQIS++ASK+ALKLLI LCSS RNRVKAV+AGAV VLIDLLLDLDSS
Sbjct: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSS 300

Query: 301 EKRACEMTLVALDLLCSCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKF 360
           EKR+CEM LV LDLLC CADGRAE+LNHAAG+A+VSKKILRVS LGSEKAVGILCS+A+F
Sbjct: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARF 360

Query: 361 SASQSVVQEMLQLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLLSNLRSS 414
           SAS+SVVQEM++LGVVTKLCFLLQV GAG KAKEKAKEILKLHGRTWRNSSCL S+LRSS
Sbjct: 361 SASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420

BLAST of Sgr026689 vs. NCBI nr
Match: KAG7033684.1 (E3 ubiquitin-protein ligase PUB22, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 646.7 bits (1667), Expect = 1.3e-181
Identity = 332/422 (78.67%), Postives = 376/422 (89.10%), Query Frame = 0

Query: 1   MAEDQVEVDTPPFFLCPISLQIMKDPVTVASGITYDRDSIEKWLFSDKHTTCPVTKAVLS 60
           MAE   E+D PPFFLCPISLQIMKDPVTVASGITYDR SIEKWLFSD H TCPVT+ VLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQVVLS 60

Query: 61  NSDLIPNHTLRRLIQAWCTVNACSGVERLPTPKPPVDRTQIVKILQDVKLSPFSQQKCLR 120
           + D+ PN TLRR+IQAWCTVNAC G+ER+PTPKPPVD  Q+V+IL D KLSPFSQQ CLR
Sbjct: 61  SFDITPNITLRRVIQAWCTVNACKGIERIPTPKPPVDCKQVVRILADAKLSPFSQQNCLR 120

Query: 121 RLRSIASANETNKRCMEAAGVVEFLAGVVCNSFDAESSFFE---------ANSDDEALSI 180
           RLRSIA+A+E+NKRCMEAAG VEFLAG+VCNS + E S  +         AN  DEALSI
Sbjct: 121 RLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGDEALSI 180

Query: 181 LYKLQISEAGLKFLLGNNGGVFVTSLTKVLQNGSYGSRAYAVMLLKSMFEVADQIKIISV 240
           L+KLQISE+GLKFLL NNGG+FV++LTK+LQNG+YGSRAY+VMLL SMFEVADQI+I+S+
Sbjct: 181 LHKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240

Query: 241 NTEFFIEIVQVLKDQISQKASKAALKLLISLCSSFRNRVKAVQAGAVQVLIDLLLDLDSS 300
           NT+FF+EIVQ+LKDQIS++ASK+ALKLLI LCSS RNRVKAV+AGAV VLIDLLLDLDSS
Sbjct: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSS 300

Query: 301 EKRACEMTLVALDLLCSCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKF 360
           EKR+CEM LV LDLLC CADGRAE+LNHAAG+A+VSKKILRVS LGSEKAVGILCS+A+F
Sbjct: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARF 360

Query: 361 SASQSVVQEMLQLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLLSNLRSS 414
           SAS+SVVQEM++LGVVTKLCFLLQV GAG KAKEKAKEILKLHGRTWRNSSCL S+LRSS
Sbjct: 361 SASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420

BLAST of Sgr026689 vs. NCBI nr
Match: XP_022925148.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita moschata])

HSP 1 Score: 645.2 bits (1663), Expect = 3.9e-181
Identity = 338/414 (81.64%), Postives = 369/414 (89.13%), Query Frame = 0

Query: 7   EVDTPPFFLCPISLQIMKDPVTVASGITYDRDSIEKWLFSDKHTTCPVTKAVLSNSDLIP 66
           EVD PPFFLCPISLQIMKDPVTVASGITYDR++IE+WLFSDKHTTCPVT+ VLS+ D+ P
Sbjct: 3   EVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFSDKHTTCPVTQVVLSDFDITP 62

Query: 67  NHTLRRLIQAWCTVNACSGVERLPTPKPPVDRTQIVKILQDVKLSPFSQQKCLRRLRSIA 126
           N+TL RLIQAWCTVN  + VER+PTPKPPVD+ Q+VKIL DVKLSPFSQQ CLRRL SIA
Sbjct: 63  NNTLSRLIQAWCTVN--NRVERIPTPKPPVDKKQVVKILADVKLSPFSQQNCLRRLESIA 122

Query: 127 SANETNKRCMEAAGVVEFLAGVVCNSFDAESSFFE-------ANSDDEALSILYKLQISE 186
           SANE NKRCMEAAG VEFL GVVCNS   ESSF +       AN  DEALSILYKLQISE
Sbjct: 123 SANEMNKRCMEAAGAVEFLVGVVCNSITIESSFPDFAFDDPLANLGDEALSILYKLQISE 182

Query: 187 AGLKFLLGNNGGVFVTSLTKVLQNGSYGSRAYAVMLLKSMFEVADQIKIISVNTEFFIEI 246
           +GLKFLL NNGG+FVT+LTK LQNG+YGSRAYA MLL+SMFEVAD+I+I S+N EFF+EI
Sbjct: 183 SGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVEI 242

Query: 247 VQVLKDQISQKASKAALKLLISLCSSFRNRVKAVQAGAVQVLIDLLLDLDSSEKRACEMT 306
           VQ+LKDQIS++ASK+ALKLLI LCSSFRNRV AVQAGAV VLIDLLLDLDSSEKR CEM 
Sbjct: 243 VQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDSSEKRLCEMI 302

Query: 307 LVALDLLCSCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKFSASQSVVQ 366
           LVALDLLC CADGRAELLNHAAG+AVVSKKILRVS LGSEKAVGILCS+A+FSASQSVVQ
Sbjct: 303 LVALDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQ 362

Query: 367 EMLQLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLLSNLRSSYP 414
           EM +LGVV KLCFLLQVVGAG+KAKEKAKEILKLHGRTWRNSSC  S+LR SYP
Sbjct: 363 EMARLGVVVKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYP 414

BLAST of Sgr026689 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 7.7e-124
Identity = 238/432 (55.09%), Postives = 306/432 (70.83%), Query Frame = 0

Query: 7   EVDTPPFFLCPISLQIMKDPVTVASGITYDRDSIEKWLFSDKHTTCPVTKAVLSNSDLIP 66
           E++ P FFLCPISL IMKDPV V++GITYDR+SIEKWLFS K  +CPVTK V++ +DL P
Sbjct: 4   EIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTP 63

Query: 67  NHTLRRLIQAWCTVNACSGVERLPTPKPPVDRTQIVKILQDVKLSPFSQQKCLRRLRSIA 126
           NHTLRRLIQ+WCT+NA  G+ER+PTPKPP+ +++I K++++   S  +Q KCL+RLR I 
Sbjct: 64  NHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQIV 123

Query: 127 SANETNKRCMEAAGVVEFLAGVVCNSFD-----------------AESSFFEANSD---- 186
           S N TNKRC+EAA V EFLA +V NS D                  +S+  E   D    
Sbjct: 124 SENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSRS 183

Query: 187 --DEALSILYKLQISEAGLKFLLGNNGGV-FVTSLTKVLQNGSYGSRAYAVMLLKSMFEV 246
             DEALS+LY L  SE  LK LL N  G   V +LTK++Q G Y SRAYA +LLK + EV
Sbjct: 184 LMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLEV 243

Query: 247 ADQIKIISVNTEFFIEIVQVLKDQISQKASKAALKLLISLCSSFRNRVKAVQAGAVQVLI 306
           AD ++II +  E F E++Q+L DQIS KA+++A+++L+  C   RNR KAV+ G + ++I
Sbjct: 244 ADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTISMII 303

Query: 307 DLLL-DLDSSEKRACEMTLVALDLLCSCADGRAELLNHAAGLAVVSKKILRVSWLGSEKA 366
           +LL+ D  SSE+R  EM +V LD+LC CA+GRAE LNH A +AVVSKKILRVS + SE+A
Sbjct: 304 ELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITSERA 363

Query: 367 VGILCSVAKFSASQSVVQEMLQLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNS 414
           V +L SV +F A+ S++QEMLQLGVV KLC +LQ V  G+K KEKAKE+LKLH R WR S
Sbjct: 364 VRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCGNKTKEKAKELLKLHARVWRES 423

BLAST of Sgr026689 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 3.8e-123
Identity = 232/409 (56.72%), Postives = 307/409 (75.06%), Query Frame = 0

Query: 7   EVDTPPFFLCPISLQIMKDPVTVASGITYDRDSIEKWLFSDKHTTCPVTKAVLSNSDLIP 66
           E++ PPFFLCPISL+IMKDPV V++GITYDRDSIEKWLF+ K  +CPVTK  ++++DL P
Sbjct: 9   EIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTP 68

Query: 67  NHTLRRLIQAWCTVNACSGVERLPTPKPPVDRTQIVKILQDVKLSPFSQQKCLRRLRSIA 126
           NHTLRRLIQ+WCT+NA  GVER+PTP+PP+ +++I K+++D   S  +Q KCL+RLR I 
Sbjct: 69  NHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQIV 128

Query: 127 SANETNKRCMEAAGVVEFLAGVVCNSFDAESSFFEANSDDEALSILYKLQISEAGLKFLL 186
           S N TNKRC+EAAGV EFLA +V N  D+E+     +  DEAL++LY L+ SE  LK LL
Sbjct: 129 SENATNKRCLEAAGVPEFLANIVSN--DSEN----GSLTDEALNLLYHLETSETVLKNLL 188

Query: 187 GN-NGGVFVTSLTKVLQNGSYGSRAYAVMLLKSMFEVADQIKIISVNTEFFIEIVQVLKD 246
            N      V SLTK++Q G Y SR YA +LLK++ EVAD ++ +++  E F E+VQ+L D
Sbjct: 189 NNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQILDD 248

Query: 247 QISQKASKAALKLLISLCSSFRNRVKAVQAGAVQVLIDLLLDLD-SSEKRACEMTLVALD 306
           +ISQKA+KAA+ +L+++C   RNR KAV+AG + V+I+LL+D   +SE+R  EM +V LD
Sbjct: 249 RISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVVLD 308

Query: 307 LLCSCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKFSASQSVVQEMLQL 366
           LLC CA+GRAE LNH A +AVV KKILRVS   S++AV +L SV +F A+ +++ EMLQL
Sbjct: 309 LLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLHEMLQL 368

Query: 367 GVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLLSNLRSSYP 414
           GVV KLC +LQ V  G K KEKAKE+LKLH R W++S CL  N+  +YP
Sbjct: 369 GVVAKLCLVLQ-VSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAYP 410

BLAST of Sgr026689 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 3.4e-63
Identity = 174/458 (37.99%), Postives = 248/458 (54.15%), Query Frame = 0

Query: 1   MAEDQVEVDTPPFFLCPISLQIMKDPVTVASGITYDRDSIEKWLFSDKHTTCPVTKAVLS 60
           M +++ E++ P +F+CPISL+IMKDPVT  SGITYDR +I KWL  +K  +CPVTK  L 
Sbjct: 1   MDQEEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLP 60

Query: 61  -NSDLIPNHTLRRLIQAWCTVNACSGVERLPTPKPPVDRTQIVKILQDVK---LSPFSQQ 120
            +SDL PNH LRRLIQ WC  N   GV R+ TP+ P  +  +V+ ++++K        ++
Sbjct: 61  LDSDLTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGRE 120

Query: 121 KCLRRLRSIASANETNKRCMEAAGVVE--FLAGVVCNSFDAESSFFEANSDDEALSILYK 180
           + L++L  +A  +  N+R M   GV +   L  V C S D E         DE+L +L+ 
Sbjct: 121 ETLQKLEVLA-MDGNNRRLMCECGVHKSLILFVVKCTSED-EDGRRRIKGLDESLRLLHL 180

Query: 181 LQISEAGLKFLLGNNGGVFVTSLTKVLQNGSYGSRAYAVMLLKSMFEVADQIKIISVNTE 240
           + I     K +L  N  V + SLT VL    + S+AY ++LL+++ E      +  +N E
Sbjct: 181 IGIPSNDAKTILMENDRV-MESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIVERLNPE 240

Query: 241 FFIEIVQVLKDQIS-----------------------------------QKASKAALKLL 300
            F  I+  LKD ++                                   ++A  AAL +L
Sbjct: 241 IFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMIL 300

Query: 301 ISLCSSFRNRVKAVQAGAVQVLIDLLLDLDSSEKRACEMTLVALDLLCSCADGRAELLNH 360
           +   S  RNR   V  GAV  LI+L +   + EKR  E+ L  L  LC CA+GRAE+L H
Sbjct: 301 LETSSWSRNRSLLVDLGAVSELIELEISY-TGEKRITELMLGVLSRLCCCANGRAEILAH 360

Query: 361 AAGLAVVSKKILRVSWLGSEKAVGILCSVAKFSASQSVVQEMLQLGVVTKLCFLLQVVGA 414
             G+AVV+K++LRVS    ++A+ IL +V+KFS    VV+EM+ +G V KLC +L  +  
Sbjct: 361 RGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLG-MDC 420

BLAST of Sgr026689 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 7.3e-50
Identity = 149/405 (36.79%), Postives = 220/405 (54.32%), Query Frame = 0

Query: 8   VDTPPFFLCPISLQIMKDPVTVASGITYDRDSIEKWLFSDKHTTCPVTKAVLSNSDLIPN 67
           +  P  F CPISL++M+DPVTV +G TYDR SIE W+    +TTCPVT+A LS+  LIPN
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPN 71

Query: 68  HTLRRLIQAWCTVNACSGVERLPTPKPPVDRTQIVKILQDVK------LSPFSQQKCLRR 127
           HTLRRLIQ WC  N  +GVER+PTPK P D T +  +L          +S  S+   LRR
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALRR 131

Query: 128 LRSIASANETNKRCMEAAGVVEFLAGVVCNSFDAESSFFEANSDD---EALSILYKLQIS 187
           LR  A  ++ N+  + A    E L  ++         F E  S +   E+L++L  L I+
Sbjct: 132 LRGFARDSDKNRVLIAAHNATEILIKIL---------FSETTSSELVSESLALLVMLPIT 191

Query: 188 EAGLKFLLGNNGGVFVTSLTKVLQNGSYGSRAYAVMLLKSM---FEVADQIKIISVNTEF 247
           E      + ++ G  V  LT++L + S  +R  A  L++ +    + AD    IS +   
Sbjct: 192 EPNQFVSISSDPG-RVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESV 251

Query: 248 FIEIVQVLKDQI-SQKASKAALKLLISLCSSFRNRVKAVQAGAVQVLID-LLLDLDSSEK 307
           F  ++ +L++ I S++A K  +K L +LCS    R  A+ AGA ++LID L  D D  + 
Sbjct: 252 FEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCD- 311

Query: 308 RACEMTLVALDLLCSCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKFSA 367
              E  L  ++LLC   +G A    HA  + ++ K ILRVS   +E A G L  +A  +A
Sbjct: 312 --TERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGAL--LALCTA 371

Query: 368 SQSVVQEMLQLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTW 399
            +   +E    GVV +L  ++Q      +AK+KA+++LKL   +W
Sbjct: 372 EERWREEAAGAGVVVQLLLMVQ-SECTERAKKKAQKLLKLLRDSW 400

BLAST of Sgr026689 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 5.8e-47
Identity = 143/411 (34.79%), Postives = 219/411 (53.28%), Query Frame = 0

Query: 8   VDTPPFFLCPISLQIMKDPVTVASGITYDRDSIEKWLFSDKHTTCPVTKAVLSNSDLIPN 67
           +  P  F CPISL +M DPVT+++G TYDR SI+ W+ +  +TTCPVT+  LS+  LIPN
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWI-AMGNTTCPVTRVALSDFTLIPN 71

Query: 68  HTLRRLIQAWCTVNACSGVERLPTPKPPVDRTQIVKILQDVK------LSPFSQQKCLRR 127
           HTLRRLIQ WC  N  +GVER+PTPK P D   +  +L          +S  S+   +RR
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIRR 131

Query: 128 LRSIASANETNKRCMEAAGVVEFLAGVVCNSFDAESSFFEANSDDEALSILYKLQISEAG 187
           LR +A  +E N+  +      E L  ++    D E++   +    E+L++L  L ++E  
Sbjct: 132 LRGLARDSEKNRVLIAGHNAREILVRIL--FADIETTSLSSELVSESLALLVLLHMTETE 191

Query: 188 LKFLLGNNGGVFVTSLTKVLQNGSYGSRAYAVMLLKSMFEVA---DQIKIISVNTEFFIE 247
            + +  +     V  +T++L + S   R  A  L++ +   A   D   IIS +   F  
Sbjct: 192 CEAVASDPSR--VGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIFEG 251

Query: 248 IVQVLKDQI-SQKASKAALKLLISLCSSFRNRVKAVQAGAVQVLID-LLLDLDSSEKRAC 307
           ++ +LK+ I S++A K  +K + +LC   + R  A+ AGA  +LID L  D D  +    
Sbjct: 252 VLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRCD---T 311

Query: 308 EMTLVALDLLCSCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKFSASQS 367
           E  L  ++LLC   +G A    HA  + ++ K ILRVS   +E A G L  +A  +A + 
Sbjct: 312 ERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGAL--LALCTAEER 371

Query: 368 VVQEMLQLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLLSN 408
              E    G+VT+L  L+Q      +AK KA+ +LKL   +W + S + S+
Sbjct: 372 CRDEAAAAGLVTQLLLLVQ-SDCTERAKRKAQMLLKLLRDSWPDDSTVHSD 411

BLAST of Sgr026689 vs. ExPASy TrEMBL
Match: A0A6J1IKG7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111478249 PE=4 SV=1)

HSP 1 Score: 656.0 bits (1691), Expect = 1.1e-184
Identity = 333/422 (78.91%), Postives = 380/422 (90.05%), Query Frame = 0

Query: 1   MAEDQVEVDTPPFFLCPISLQIMKDPVTVASGITYDRDSIEKWLFSDKHTTCPVTKAVLS 60
           MAE   E+D PPFFLCPISLQIMKDPVTVASGITYDR+SIE+WLFSD+H TCPVT+ VLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVLS 60

Query: 61  NSDLIPNHTLRRLIQAWCTVNACSGVERLPTPKPPVDRTQIVKILQDVKLSPFSQQKCLR 120
           + D+ PNHT+RR+IQAWCT+NAC G+ER+PTPKPP+D  Q+V+IL D KLSPFSQQ CLR
Sbjct: 61  SFDITPNHTIRRVIQAWCTINACKGIERIPTPKPPIDCKQVVRILADAKLSPFSQQNCLR 120

Query: 121 RLRSIASANETNKRCMEAAGVVEFLAGVVCNSFDAESSFFE---------ANSDDEALSI 180
           RLRSIA+A+E+NKRCMEAAG VEFLAG+VCNS + E S  +         AN  DEALSI
Sbjct: 121 RLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEALSI 180

Query: 181 LYKLQISEAGLKFLLGNNGGVFVTSLTKVLQNGSYGSRAYAVMLLKSMFEVADQIKIISV 240
           LYKLQISE+GLKFLL NNGG+FV++LTK+LQNG+YGSRAY+VMLL SMFEVADQI+I+S+
Sbjct: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQILSL 240

Query: 241 NTEFFIEIVQVLKDQISQKASKAALKLLISLCSSFRNRVKAVQAGAVQVLIDLLLDLDSS 300
           NT+FFIEIVQ+LKDQIS++ASK+ALKLLI LCSS RNRVKAVQAGA+ VLIDLLLDLDSS
Sbjct: 241 NTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLDSS 300

Query: 301 EKRACEMTLVALDLLCSCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKF 360
           EKR+CEM LV LDLLC CADGRAE+LNHAAG+AVVSKKILRVS LGSEKAVGILCS+A+F
Sbjct: 301 EKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIARF 360

Query: 361 SASQSVVQEMLQLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLLSNLRSS 414
           SAS+SVVQEM++LGVVTKLCFLLQVVGAG KAKEKAKEILKLHGRTWRNSSCL S+LRSS
Sbjct: 361 SASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420

BLAST of Sgr026689 vs. ExPASy TrEMBL
Match: A0A6J1GGQ8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111453790 PE=4 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 6.4e-182
Identity = 330/422 (78.20%), Postives = 376/422 (89.10%), Query Frame = 0

Query: 1   MAEDQVEVDTPPFFLCPISLQIMKDPVTVASGITYDRDSIEKWLFSDKHTTCPVTKAVLS 60
           MAE   E+D PPFFLCPISLQIMKDPVTVASGITYDR+SIE+WLFSD+H TCP+T+ VLS
Sbjct: 1   MAELDQELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVLS 60

Query: 61  NSDLIPNHTLRRLIQAWCTVNACSGVERLPTPKPPVDRTQIVKILQDVKLSPFSQQKCLR 120
           N D+ PNHTLRR+IQAWCTVNAC GVER+PTPKPPVD  Q+V+IL D KLS FSQQ CLR
Sbjct: 61  NFDITPNHTLRRVIQAWCTVNACKGVERIPTPKPPVDCKQVVRILADAKLSSFSQQNCLR 120

Query: 121 RLRSIASANETNKRCMEAAGVVEFLAGVVCNSFDAESSFFE---------ANSDDEALSI 180
           RL+SIA+A+E+NKRCMEAAG VEFLAG+VCNS + E S  +         AN   EALSI
Sbjct: 121 RLQSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFEALSI 180

Query: 181 LYKLQISEAGLKFLLGNNGGVFVTSLTKVLQNGSYGSRAYAVMLLKSMFEVADQIKIISV 240
           LYKLQISE+GLKFLL NNGG+FV++LTK+LQNG+YGSR Y+VMLL SMFEVADQI+I+S+
Sbjct: 181 LYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQILSL 240

Query: 241 NTEFFIEIVQVLKDQISQKASKAALKLLISLCSSFRNRVKAVQAGAVQVLIDLLLDLDSS 300
           NT+FF+EIVQ+LKDQIS++ASK+ALKLLI LCSS RNRVKAV+AGAV VLIDLLLDLDSS
Sbjct: 241 NTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLDSS 300

Query: 301 EKRACEMTLVALDLLCSCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKF 360
           EKR+CEM LV LDLLC CADGRAE+LNHAAG+A+VSKKILRVS LGSEKAVGILCS+A+F
Sbjct: 301 EKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIARF 360

Query: 361 SASQSVVQEMLQLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLLSNLRSS 414
           SAS+SVVQEM++LGVVTKLCFLLQV GAG KAKEKAKEILKLHGRTWRNSSCL S+LRSS
Sbjct: 361 SASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSLRSS 420

BLAST of Sgr026689 vs. ExPASy TrEMBL
Match: A0A6J1EEF1 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432478 PE=4 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 1.9e-181
Identity = 338/414 (81.64%), Postives = 369/414 (89.13%), Query Frame = 0

Query: 7   EVDTPPFFLCPISLQIMKDPVTVASGITYDRDSIEKWLFSDKHTTCPVTKAVLSNSDLIP 66
           EVD PPFFLCPISLQIMKDPVTVASGITYDR++IE+WLFSDKHTTCPVT+ VLS+ D+ P
Sbjct: 3   EVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFSDKHTTCPVTQVVLSDFDITP 62

Query: 67  NHTLRRLIQAWCTVNACSGVERLPTPKPPVDRTQIVKILQDVKLSPFSQQKCLRRLRSIA 126
           N+TL RLIQAWCTVN  + VER+PTPKPPVD+ Q+VKIL DVKLSPFSQQ CLRRL SIA
Sbjct: 63  NNTLSRLIQAWCTVN--NRVERIPTPKPPVDKKQVVKILADVKLSPFSQQNCLRRLESIA 122

Query: 127 SANETNKRCMEAAGVVEFLAGVVCNSFDAESSFFE-------ANSDDEALSILYKLQISE 186
           SANE NKRCMEAAG VEFL GVVCNS   ESSF +       AN  DEALSILYKLQISE
Sbjct: 123 SANEMNKRCMEAAGAVEFLVGVVCNSITIESSFPDFAFDDPLANLGDEALSILYKLQISE 182

Query: 187 AGLKFLLGNNGGVFVTSLTKVLQNGSYGSRAYAVMLLKSMFEVADQIKIISVNTEFFIEI 246
           +GLKFLL NNGG+FVT+LTK LQNG+YGSRAYA MLL+SMFEVAD+I+I S+N EFF+EI
Sbjct: 183 SGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVEI 242

Query: 247 VQVLKDQISQKASKAALKLLISLCSSFRNRVKAVQAGAVQVLIDLLLDLDSSEKRACEMT 306
           VQ+LKDQIS++ASK+ALKLLI LCSSFRNRV AVQAGAV VLIDLLLDLDSSEKR CEM 
Sbjct: 243 VQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDSSEKRLCEMI 302

Query: 307 LVALDLLCSCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKFSASQSVVQ 366
           LVALDLLC CADGRAELLNHAAG+AVVSKKILRVS LGSEKAVGILCS+A+FSASQSVVQ
Sbjct: 303 LVALDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQ 362

Query: 367 EMLQLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLLSNLRSSYP 414
           EM +LGVV KLCFLLQVVGAG+KAKEKAKEILKLHGRTWRNSSC  S+LR SYP
Sbjct: 363 EMARLGVVVKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYP 414

BLAST of Sgr026689 vs. ExPASy TrEMBL
Match: A0A6J1HP11 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466021 PE=4 SV=1)

HSP 1 Score: 635.2 bits (1637), Expect = 1.9e-178
Identity = 330/414 (79.71%), Postives = 368/414 (88.89%), Query Frame = 0

Query: 7   EVDTPPFFLCPISLQIMKDPVTVASGITYDRDSIEKWLFSDKHTTCPVTKAVLSNSDLIP 66
           EVD PPFFLCPISLQIMKDPVTVASGITYDR++IE+WLF DKHTTCPVT+ VLS+ D+ P
Sbjct: 3   EVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDITP 62

Query: 67  NHTLRRLIQAWCTVNACSGVERLPTPKPPVDRTQIVKILQDVKLSPFSQQKCLRRLRSIA 126
           N+TL RLIQAW T+N   GV+R+PTPKPP+D+ Q++KIL +VKLSPFSQQ CL+RLRSIA
Sbjct: 63  NNTLSRLIQAWSTLN--DGVDRIPTPKPPIDKKQVIKILANVKLSPFSQQNCLQRLRSIA 122

Query: 127 SANETNKRCMEAAGVVEFLAGVVCNSFDAESSFFE-------ANSDDEALSILYKLQISE 186
           S NETNKRCMEAAG VEFL GV CNS   ES+F +       AN  DEALSILYKLQISE
Sbjct: 123 STNETNKRCMEAAGAVEFLVGVACNSITIESNFQDFAFDDPLANLGDEALSILYKLQISE 182

Query: 187 AGLKFLLGNNGGVFVTSLTKVLQNGSYGSRAYAVMLLKSMFEVADQIKIISVNTEFFIEI 246
           +GLKFLL NNGG+FVT+LTK LQNG+YGSRAYA MLL+SMFEVAD+I+I S+N EFF+EI
Sbjct: 183 SGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVEI 242

Query: 247 VQVLKDQISQKASKAALKLLISLCSSFRNRVKAVQAGAVQVLIDLLLDLDSSEKRACEMT 306
           VQ+LKDQIS++ASK+ALKLLI LCSSFRNRV AVQAGAV VLIDLLLDLD SEKR CEM 
Sbjct: 243 VQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDLSEKRLCEMI 302

Query: 307 LVALDLLCSCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKFSASQSVVQ 366
           LVALDLLCSCADGRAELLNHAAG+AVVSKKILRVS LGSEKAVGILCS+A+FSASQSVVQ
Sbjct: 303 LVALDLLCSCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVVQ 362

Query: 367 EMLQLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLLSNLRSSYP 414
           EM++LGVV KLCFLLQVVGAG+KAKEKAKEILKLHGRTWRNSSC  S+LR SYP
Sbjct: 363 EMVRLGVVMKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSLRPSYP 414

BLAST of Sgr026689 vs. ExPASy TrEMBL
Match: A0A5A7UVZ1 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold654G00730 PE=4 SV=1)

HSP 1 Score: 615.1 bits (1585), Expect = 2.1e-172
Identity = 323/420 (76.90%), Postives = 369/420 (87.86%), Query Frame = 0

Query: 3   EDQVEVDTPPFFLCPISLQIMKDPVTVASGITYDRDSIEKWLFSDKHTTCPVTKAVLSNS 62
           +DQV VD PP+FLCPISLQIMKDPVT+ASGITYDR+SIEKWLFSDKH TCPV+  VLS+ 
Sbjct: 7   QDQV-VDPPPYFLCPISLQIMKDPVTIASGITYDRESIEKWLFSDKHKTCPVSHIVLSDF 66

Query: 63  DLIPNHTLRRLIQAWCTVNACSGVERLPTPKPPVDRTQIVKILQDVKLSPFSQQKCLRRL 122
           D+ PNHTLRR+IQAWCTVNA  GVER+PTPKPPVDR Q+V+IL D KLSPFSQ+ CL RL
Sbjct: 67  DVTPNHTLRRVIQAWCTVNASKGVERIPTPKPPVDREQVVRILADAKLSPFSQKNCLCRL 126

Query: 123 RSIASANETNKRCMEAAGVVEFLAGVVCNS-----FDAESSFFE---ANSDDEALSILYK 182
           RSIA+ANE+NKRCME+AGVVEFLAGVVCNS     +  E   F+   ANS DEAL IL+K
Sbjct: 127 RSIAAANESNKRCMESAGVVEFLAGVVCNSTTNMEYGLEDYAFDDMSANSADEALIILHK 186

Query: 183 LQISEAGLKFLLGNNGGVFVTSLTKVLQNGSYGSRAYAVMLLKSMFEVADQIKIISVNTE 242
           LQISE+ LKFLL NNGG+F+++LTK+LQN SY SR+Y+VMLL SMFEVADQI+I ++  +
Sbjct: 187 LQISESSLKFLLSNNGGIFISTLTKILQNRSYSSRSYSVMLLNSMFEVADQIQIQNLTAD 246

Query: 243 FFIEIVQVLKDQISQKASKAALKLLISLCSSFRNRVKAVQAGAVQVLIDLLLDLDSSE-K 302
           FFIEI+Q+LKDQIS++ASK+ALKLLI++ SSFRNRVKAVQAGAV VLIDLLLDLDSSE K
Sbjct: 247 FFIEIIQILKDQISKQASKSALKLLIAVSSSFRNRVKAVQAGAVPVLIDLLLDLDSSENK 306

Query: 303 RACEMTLVALDLLCSCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKFSA 362
           R CEM LV LD LC CADGRAELLNHAAG+AVVSKKILR+S +GSEKAVGIL S+A+FS 
Sbjct: 307 RLCEMILVLLDQLCGCADGRAELLNHAAGIAVVSKKILRISTVGSEKAVGILWSIARFSG 366

Query: 363 SQSVVQEMLQLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLLSNLRSSYP 414
           SQSVVQEM+++GVVTKLCFLLQVVGAG KAKEKAKEILKLHGRTWRNSSCL S LRS+YP
Sbjct: 367 SQSVVQEMVRIGVVTKLCFLLQVVGAGVKAKEKAKEILKLHGRTWRNSSCLPSTLRSAYP 425

BLAST of Sgr026689 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 445.3 bits (1144), Expect = 5.4e-125
Identity = 238/432 (55.09%), Postives = 306/432 (70.83%), Query Frame = 0

Query: 7   EVDTPPFFLCPISLQIMKDPVTVASGITYDRDSIEKWLFSDKHTTCPVTKAVLSNSDLIP 66
           E++ P FFLCPISL IMKDPV V++GITYDR+SIEKWLFS K  +CPVTK V++ +DL P
Sbjct: 4   EIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTP 63

Query: 67  NHTLRRLIQAWCTVNACSGVERLPTPKPPVDRTQIVKILQDVKLSPFSQQKCLRRLRSIA 126
           NHTLRRLIQ+WCT+NA  G+ER+PTPKPP+ +++I K++++   S  +Q KCL+RLR I 
Sbjct: 64  NHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQIV 123

Query: 127 SANETNKRCMEAAGVVEFLAGVVCNSFD-----------------AESSFFEANSD---- 186
           S N TNKRC+EAA V EFLA +V NS D                  +S+  E   D    
Sbjct: 124 SENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSRS 183

Query: 187 --DEALSILYKLQISEAGLKFLLGNNGGV-FVTSLTKVLQNGSYGSRAYAVMLLKSMFEV 246
             DEALS+LY L  SE  LK LL N  G   V +LTK++Q G Y SRAYA +LLK + EV
Sbjct: 184 LMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLEV 243

Query: 247 ADQIKIISVNTEFFIEIVQVLKDQISQKASKAALKLLISLCSSFRNRVKAVQAGAVQVLI 306
           AD ++II +  E F E++Q+L DQIS KA+++A+++L+  C   RNR KAV+ G + ++I
Sbjct: 244 ADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTISMII 303

Query: 307 DLLL-DLDSSEKRACEMTLVALDLLCSCADGRAELLNHAAGLAVVSKKILRVSWLGSEKA 366
           +LL+ D  SSE+R  EM +V LD+LC CA+GRAE LNH A +AVVSKKILRVS + SE+A
Sbjct: 304 ELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITSERA 363

Query: 367 VGILCSVAKFSASQSVVQEMLQLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNS 414
           V +L SV +F A+ S++QEMLQLGVV KLC +LQ V  G+K KEKAKE+LKLH R WR S
Sbjct: 364 VRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCGNKTKEKAKELLKLHARVWRES 423

BLAST of Sgr026689 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 443.0 bits (1138), Expect = 2.7e-124
Identity = 232/409 (56.72%), Postives = 307/409 (75.06%), Query Frame = 0

Query: 7   EVDTPPFFLCPISLQIMKDPVTVASGITYDRDSIEKWLFSDKHTTCPVTKAVLSNSDLIP 66
           E++ PPFFLCPISL+IMKDPV V++GITYDRDSIEKWLF+ K  +CPVTK  ++++DL P
Sbjct: 9   EIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTP 68

Query: 67  NHTLRRLIQAWCTVNACSGVERLPTPKPPVDRTQIVKILQDVKLSPFSQQKCLRRLRSIA 126
           NHTLRRLIQ+WCT+NA  GVER+PTP+PP+ +++I K+++D   S  +Q KCL+RLR I 
Sbjct: 69  NHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQIV 128

Query: 127 SANETNKRCMEAAGVVEFLAGVVCNSFDAESSFFEANSDDEALSILYKLQISEAGLKFLL 186
           S N TNKRC+EAAGV EFLA +V N  D+E+     +  DEAL++LY L+ SE  LK LL
Sbjct: 129 SENATNKRCLEAAGVPEFLANIVSN--DSEN----GSLTDEALNLLYHLETSETVLKNLL 188

Query: 187 GN-NGGVFVTSLTKVLQNGSYGSRAYAVMLLKSMFEVADQIKIISVNTEFFIEIVQVLKD 246
            N      V SLTK++Q G Y SR YA +LLK++ EVAD ++ +++  E F E+VQ+L D
Sbjct: 189 NNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQILDD 248

Query: 247 QISQKASKAALKLLISLCSSFRNRVKAVQAGAVQVLIDLLLDLD-SSEKRACEMTLVALD 306
           +ISQKA+KAA+ +L+++C   RNR KAV+AG + V+I+LL+D   +SE+R  EM +V LD
Sbjct: 249 RISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVVLD 308

Query: 307 LLCSCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKFSASQSVVQEMLQL 366
           LLC CA+GRAE LNH A +AVV KKILRVS   S++AV +L SV +F A+ +++ EMLQL
Sbjct: 309 LLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLHEMLQL 368

Query: 367 GVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLLSNLRSSYP 414
           GVV KLC +LQ V  G K KEKAKE+LKLH R W++S CL  N+  +YP
Sbjct: 369 GVVAKLCLVLQ-VSCGGKTKEKAKELLKLHARVWKDSPCLPKNMILAYP 410

BLAST of Sgr026689 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 243.8 bits (621), Expect = 2.4e-64
Identity = 174/458 (37.99%), Postives = 248/458 (54.15%), Query Frame = 0

Query: 1   MAEDQVEVDTPPFFLCPISLQIMKDPVTVASGITYDRDSIEKWLFSDKHTTCPVTKAVLS 60
           M +++ E++ P +F+CPISL+IMKDPVT  SGITYDR +I KWL  +K  +CPVTK  L 
Sbjct: 15  MDQEEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLP 74

Query: 61  -NSDLIPNHTLRRLIQAWCTVNACSGVERLPTPKPPVDRTQIVKILQDVK---LSPFSQQ 120
            +SDL PNH LRRLIQ WC  N   GV R+ TP+ P  +  +V+ ++++K        ++
Sbjct: 75  LDSDLTPNHMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGRE 134

Query: 121 KCLRRLRSIASANETNKRCMEAAGVVE--FLAGVVCNSFDAESSFFEANSDDEALSILYK 180
           + L++L  +A  +  N+R M   GV +   L  V C S D E         DE+L +L+ 
Sbjct: 135 ETLQKLEVLA-MDGNNRRLMCECGVHKSLILFVVKCTSED-EDGRRRIKGLDESLRLLHL 194

Query: 181 LQISEAGLKFLLGNNGGVFVTSLTKVLQNGSYGSRAYAVMLLKSMFEVADQIKIISVNTE 240
           + I     K +L  N  V + SLT VL    + S+AY ++LL+++ E      +  +N E
Sbjct: 195 IGIPSNDAKTILMENDRV-MESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIVERLNPE 254

Query: 241 FFIEIVQVLKDQIS-----------------------------------QKASKAALKLL 300
            F  I+  LKD ++                                   ++A  AAL +L
Sbjct: 255 IFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMIL 314

Query: 301 ISLCSSFRNRVKAVQAGAVQVLIDLLLDLDSSEKRACEMTLVALDLLCSCADGRAELLNH 360
           +   S  RNR   V  GAV  LI+L +   + EKR  E+ L  L  LC CA+GRAE+L H
Sbjct: 315 LETSSWSRNRSLLVDLGAVSELIELEISY-TGEKRITELMLGVLSRLCCCANGRAEILAH 374

Query: 361 AAGLAVVSKKILRVSWLGSEKAVGILCSVAKFSASQSVVQEMLQLGVVTKLCFLLQVVGA 414
             G+AVV+K++LRVS    ++A+ IL +V+KFS    VV+EM+ +G V KLC +L  +  
Sbjct: 375 RGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLG-MDC 434

BLAST of Sgr026689 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 199.5 bits (506), Expect = 5.2e-51
Identity = 149/405 (36.79%), Postives = 220/405 (54.32%), Query Frame = 0

Query: 8   VDTPPFFLCPISLQIMKDPVTVASGITYDRDSIEKWLFSDKHTTCPVTKAVLSNSDLIPN 67
           +  P  F CPISL++M+DPVTV +G TYDR SIE W+    +TTCPVT+A LS+  LIPN
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPN 71

Query: 68  HTLRRLIQAWCTVNACSGVERLPTPKPPVDRTQIVKILQDVK------LSPFSQQKCLRR 127
           HTLRRLIQ WC  N  +GVER+PTPK P D T +  +L          +S  S+   LRR
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALRR 131

Query: 128 LRSIASANETNKRCMEAAGVVEFLAGVVCNSFDAESSFFEANSDD---EALSILYKLQIS 187
           LR  A  ++ N+  + A    E L  ++         F E  S +   E+L++L  L I+
Sbjct: 132 LRGFARDSDKNRVLIAAHNATEILIKIL---------FSETTSSELVSESLALLVMLPIT 191

Query: 188 EAGLKFLLGNNGGVFVTSLTKVLQNGSYGSRAYAVMLLKSM---FEVADQIKIISVNTEF 247
           E      + ++ G  V  LT++L + S  +R  A  L++ +    + AD    IS +   
Sbjct: 192 EPNQFVSISSDPG-RVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESV 251

Query: 248 FIEIVQVLKDQI-SQKASKAALKLLISLCSSFRNRVKAVQAGAVQVLID-LLLDLDSSEK 307
           F  ++ +L++ I S++A K  +K L +LCS    R  A+ AGA ++LID L  D D  + 
Sbjct: 252 FEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCD- 311

Query: 308 RACEMTLVALDLLCSCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKFSA 367
              E  L  ++LLC   +G A    HA  + ++ K ILRVS   +E A G L  +A  +A
Sbjct: 312 --TERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGAL--LALCTA 371

Query: 368 SQSVVQEMLQLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTW 399
            +   +E    GVV +L  ++Q      +AK+KA+++LKL   +W
Sbjct: 372 EERWREEAAGAGVVVQLLLMVQ-SECTERAKKKAQKLLKLLRDSW 400

BLAST of Sgr026689 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 189.9 bits (481), Expect = 4.1e-48
Identity = 143/411 (34.79%), Postives = 219/411 (53.28%), Query Frame = 0

Query: 8   VDTPPFFLCPISLQIMKDPVTVASGITYDRDSIEKWLFSDKHTTCPVTKAVLSNSDLIPN 67
           +  P  F CPISL +M DPVT+++G TYDR SI+ W+ +  +TTCPVT+  LS+  LIPN
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWI-AMGNTTCPVTRVALSDFTLIPN 71

Query: 68  HTLRRLIQAWCTVNACSGVERLPTPKPPVDRTQIVKILQDVK------LSPFSQQKCLRR 127
           HTLRRLIQ WC  N  +GVER+PTPK P D   +  +L          +S  S+   +RR
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIRR 131

Query: 128 LRSIASANETNKRCMEAAGVVEFLAGVVCNSFDAESSFFEANSDDEALSILYKLQISEAG 187
           LR +A  +E N+  +      E L  ++    D E++   +    E+L++L  L ++E  
Sbjct: 132 LRGLARDSEKNRVLIAGHNAREILVRIL--FADIETTSLSSELVSESLALLVLLHMTETE 191

Query: 188 LKFLLGNNGGVFVTSLTKVLQNGSYGSRAYAVMLLKSMFEVA---DQIKIISVNTEFFIE 247
            + +  +     V  +T++L + S   R  A  L++ +   A   D   IIS +   F  
Sbjct: 192 CEAVASDPSR--VGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIFEG 251

Query: 248 IVQVLKDQI-SQKASKAALKLLISLCSSFRNRVKAVQAGAVQVLID-LLLDLDSSEKRAC 307
           ++ +LK+ I S++A K  +K + +LC   + R  A+ AGA  +LID L  D D  +    
Sbjct: 252 VLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRCD---T 311

Query: 308 EMTLVALDLLCSCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKFSASQS 367
           E  L  ++LLC   +G A    HA  + ++ K ILRVS   +E A G L  +A  +A + 
Sbjct: 312 ERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGAL--LALCTAEER 371

Query: 368 VVQEMLQLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLLSN 408
              E    G+VT+L  L+Q      +AK KA+ +LKL   +W + S + S+
Sbjct: 372 CRDEAAAAGLVTQLLLLVQ-SDCTERAKRKAQMLLKLLRDSWPDDSTVHSD 411

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022978192.12.2e-18478.91E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima][more]
XP_023543358.16.3e-18479.15E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo][more]
XP_022950790.11.3e-18178.20E3 ubiquitin-protein ligase PUB22-like [Cucurbita moschata][more]
KAG7033684.11.3e-18178.67E3 ubiquitin-protein ligase PUB22, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022925148.13.9e-18181.64E3 ubiquitin-protein ligase PUB22-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9SVC67.7e-12455.09E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ... [more]
Q84TG33.8e-12356.72E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Q9SF153.4e-6337.99E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ... [more]
Q9LT797.3e-5036.79U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q9FXA45.8e-4734.79U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1IKG71.1e-18478.91RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111478249 P... [more]
A0A6J1GGQ86.4e-18278.20RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111453790... [more]
A0A6J1EEF11.9e-18181.64RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432478... [more]
A0A6J1HP111.9e-17879.71RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466021 P... [more]
A0A5A7UVZ12.1e-17276.90RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
Match NameE-valueIdentityDescription
AT3G52450.15.4e-12555.09plant U-box 22 [more]
AT2G35930.12.7e-12456.72plant U-box 23 [more]
AT3G11840.12.4e-6437.99plant U-box 24 [more]
AT3G19380.15.2e-5136.79plant U-box 25 [more]
AT1G49780.14.1e-4834.79plant U-box 26 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 13..77
e-value: 2.4E-32
score: 123.5
IPR003613U box domainPFAMPF04564U-boxcoord: 11..79
e-value: 1.5E-21
score: 76.3
IPR003613U box domainPROSITEPS51698U_BOXcoord: 9..84
score: 41.691551
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 1..86
e-value: 1.1E-29
score: 104.4
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 98..409
e-value: 4.1E-17
score: 64.0
NoneNo IPR availablePANTHERPTHR22849:SF132E3 UBIQUITIN-PROTEIN LIGASE PUB23coord: 7..413
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 7..413
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 14..56
e-value: 4.42546E-22
score: 86.5186
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 4..83
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 101..375

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr026689.1Sgr026689.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity