Sgr026245 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr026245 vs. NCBI nr
Match: XP_038881674.1 (subtilisin-like protease SBT1.7 [Benincasa hispida])

HSP 1 Score: 1425.6 bits (3689), Expect = 0.0e+00
Identity = 715/761 (93.96%), Postives = 735/761 (96.58%), Query Frame = 0

Query: 4   MAKPVRIFVLLCFVFGVSMAAAEKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTAEM 63
           MA P+ IF+LLCF    SMAAA+KKTYIVHMAKYQMPESFEHHLHWYDSSL+SVSDTAEM
Sbjct: 1   MANPLWIFLLLCFFSVPSMAAAQKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDTAEM 60

Query: 64  IYAYNNVVHGFSTRLTAEEAQRLEGQPGILAVVPEMRYELHTTRTPQFLGLDKNADLYPE 123
           IYAYNNVVHGFSTRLTAEEAQRLE QPGILAVVPEMRYELHTTRTPQFLGLDKNA+LYPE
Sbjct: 61  IYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNANLYPE 120

Query: 124 SNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARFF 183
           SNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARFF
Sbjct: 121 SNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARFF 180

Query: 184 SKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARAR 243
           SKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARAR
Sbjct: 181 SKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARAR 240

Query: 244 VAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKGI 303
           VAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGG+SDYYKDSVATGAFAAMEKGI
Sbjct: 241 VAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVATGAFAAMEKGI 300

Query: 304 LISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAFVSLGDGKNFSGVSLYRGKPLPG 363
           L+SCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPA+VSLGD KNFSGVSLYRGK LPG
Sbjct: 301 LVSCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKALPG 360

Query: 364 TLLPFIYAANASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMV 423
           TLLPFIYAANASN+ NGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMV
Sbjct: 361 TLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMV 420

Query: 424 LANTAANGEELVADAHLLPATAVGQKSGDMIRKYLISEPNPTVTILFEGTKLGIEPSPVV 483
           LANTAANGEELVAD+HLLPATAVGQKSGD++RKYLISEPNPTVTILFEGTKLGIEPSPVV
Sbjct: 421 LANTAANGEELVADSHLLPATAVGQKSGDIMRKYLISEPNPTVTILFEGTKLGIEPSPVV 480

Query: 484 AAFSSRGPNSITPQLLKPDIIAPGVNIIAGWSKSVGPSGLPIDDRRVDFNIISGTSMSCP 543
           AAFSSRGPNSITPQLLKPDIIAPGVNI+AGWSKSVGPSGLPIDDRRVDFNIISGTSMSCP
Sbjct: 481 AAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLPIDDRRVDFNIISGTSMSCP 540

Query: 544 HVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKLQDIATGKPSTPFDHGAGHVDP 603
           HVSGLAALIKGAHPDWSPAAIRSALMTTAYTAY+NGQK+QDIATGKPSTPFDHGAGHVDP
Sbjct: 541 HVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYRNGQKIQDIATGKPSTPFDHGAGHVDP 600

Query: 604 VSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARREFTCDAGKKYSVNDLNYPSFAAV 663
           VSALNPGLVYDLTVDDYLNFLCALNYT SQINSLARR+FTCD+ KKYSVNDLNYPSFA V
Sbjct: 601 VSALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARRDFTCDSKKKYSVNDLNYPSFAVV 660

Query: 664 FEGALGG---GSGVVKHTRTLTNVGSPGTYKVSITSETKLVKISVEPESLTFAEANEKKS 723
           F+G LGG   GS VVKHTRTLTNVGSPGTYKVSI+SETKLVKISVEPESL+F  ANEKKS
Sbjct: 661 FDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTGANEKKS 720

Query: 724 YTATFATTTGSAAAPSTEGFGRIEWSDGKHVVGSPIAFSWT 762
           YT TF T TGSAA PS EGFGRIEWSDGK VVGSPIAFSWT
Sbjct: 721 YTVTFTTVTGSAAPPSAEGFGRIEWSDGKQVVGSPIAFSWT 761

BLAST of Sgr026245 vs. NCBI nr
Match: XP_022950492.1 (subtilisin-like protease SBT1.7 [Cucurbita moschata])

HSP 1 Score: 1402.9 bits (3630), Expect = 0.0e+00
Identity = 699/764 (91.49%), Postives = 735/764 (96.20%), Query Frame = 0

Query: 4   MAKPVRIFVLLCFVFGVSMA---AAEKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDT 63
           M KPV IF+L CF  G  MA   AA+KKTY+VHMAKYQMP++FEHHLHWYD+SLKSVSDT
Sbjct: 1   MVKPVWIFLLFCFFSGPFMAAAVAAQKKTYVVHMAKYQMPQTFEHHLHWYDASLKSVSDT 60

Query: 64  AEMIYAYNNVVHGFSTRLTAEEAQRLEGQPGILAVVPEMRYELHTTRTPQFLGLDKNADL 123
           A+MIYAY  VVHGFSTRLTAEEAQRLE QPG+LAVVPEMRYELHTTRTPQFLGLDKNADL
Sbjct: 61  ADMIYAYKTVVHGFSTRLTAEEAQRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADL 120

Query: 124 YPESNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 183
           YPES S SEVIIGVLDTGVWPESKSFDD+GLGPVPSSWKGECESGTNFSASNCNRKLIGA
Sbjct: 121 YPESTSTSEVIIGVLDTGVWPESKSFDDSGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180

Query: 184 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 243
           RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA
Sbjct: 181 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240

Query: 244 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAME 303
           RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGG+SDYYKDSVA+GAFAAME
Sbjct: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300

Query: 304 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAFVSLGDGKNFSGVSLYRGKP 363
           KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPA+VS+GDGKNFSGVSLYRGK 
Sbjct: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKS 360

Query: 364 LPGTLLPFIYAANASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGI 423
           LPGTLLPFIYAANASN++NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGI
Sbjct: 361 LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGI 420

Query: 424 GMVLANTAANGEELVADAHLLPATAVGQKSGDMIRKYLISEPNPTVTILFEGTKLGIEPS 483
           GMVLANTAANGEELVAD+HLLPA+AVGQKSGD+IRKYLIS+P PTVTILFEGTKLGIEPS
Sbjct: 421 GMVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPS 480

Query: 484 PVVAAFSSRGPNSITPQLLKPDIIAPGVNIIAGWSKSVGPSGLPIDDRRVDFNIISGTSM 543
           PVVAAFSSRGPNSITPQ+LKPDIIAPGVNI+AGWSK+VGPSGLPID+RRVDFNIISGTSM
Sbjct: 481 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLPIDERRVDFNIISGTSM 540

Query: 544 SCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKLQDIATGKPSTPFDHGAGH 603
           SCPHVSGLAALIKGAH DWSPAAIRSALMTTAYTAYKNGQK+QDIATGKPSTPFDHGAGH
Sbjct: 541 SCPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 600

Query: 604 VDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARREFTCDAGKKYSVNDLNYPSF 663
           VDPVSALNPGLVYDLTVDDYL+FLCALNYTSSQINSLARR++TCD+GKKYSVN+LNYPSF
Sbjct: 601 VDPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSF 660

Query: 664 AAVFEGALGG---GSGVVKHTRTLTNVGSPGTYKVSITSETKLVKISVEPESLTFAEANE 723
           A VF+G LGG   GS VVKHTRTLTNVGSPGTYKVSI+SETKLVKISVEPESL+F +ANE
Sbjct: 661 AVVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 720

Query: 724 KKSYTATFATTTGSAAAPSTEGFGRIEWSDGKHVVGSPIAFSWT 762
           KKSYT TF TTTGS+A PS EGFGRIEW+DGKHVVGSPIAFSWT
Sbjct: 721 KKSYTVTFTTTTGSSAPPSAEGFGRIEWTDGKHVVGSPIAFSWT 764

BLAST of Sgr026245 vs. NCBI nr
Match: KAG6604148.1 (Subtilisin-like protease 1.7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1402.1 bits (3628), Expect = 0.0e+00
Identity = 699/764 (91.49%), Postives = 734/764 (96.07%), Query Frame = 0

Query: 4   MAKPVRIFVLLCFVFGVSMA---AAEKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDT 63
           M KPV IF+L CF  G  MA   AA+KKTY+VHMAKYQMP++FEHHLHWYD+SLKSVSDT
Sbjct: 1   MEKPVWIFLLFCFFSGPFMAAAVAAQKKTYVVHMAKYQMPQTFEHHLHWYDASLKSVSDT 60

Query: 64  AEMIYAYNNVVHGFSTRLTAEEAQRLEGQPGILAVVPEMRYELHTTRTPQFLGLDKNADL 123
           A+MIYAY  VVHGFSTRLTAEEAQRLE QPG+LAVVPEMRYELHTTRTPQFLGLDKNADL
Sbjct: 61  ADMIYAYKTVVHGFSTRLTAEEAQRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADL 120

Query: 124 YPESNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 183
           YPES S SEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA
Sbjct: 121 YPESTSTSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180

Query: 184 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 243
           RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA
Sbjct: 181 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240

Query: 244 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAME 303
           RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGG+SDYYKDSVA+GAFAAME
Sbjct: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300

Query: 304 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAFVSLGDGKNFSGVSLYRGKP 363
           KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPA+VS+GDGKNFSGVSLYRGK 
Sbjct: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKS 360

Query: 364 LPGTLLPFIYAANASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGI 423
           LPGTLLPFIYAANASN++NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGI
Sbjct: 361 LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGI 420

Query: 424 GMVLANTAANGEELVADAHLLPATAVGQKSGDMIRKYLISEPNPTVTILFEGTKLGIEPS 483
           GMVLANTAANGEELVAD+HLLPA+AVGQKSGD+IRKYLIS+P PTVTILFEGTKLGIEPS
Sbjct: 421 GMVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPS 480

Query: 484 PVVAAFSSRGPNSITPQLLKPDIIAPGVNIIAGWSKSVGPSGLPIDDRRVDFNIISGTSM 543
           PVVAAFSSRGPNSITPQ+LKPDIIAPGVNI+AGWSK+VGPSGLPID+RRVDFNIISGTSM
Sbjct: 481 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLPIDERRVDFNIISGTSM 540

Query: 544 SCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKLQDIATGKPSTPFDHGAGH 603
           SCPHVSGLAALIKGAH DWSPAAIRSALMTTAYTAYKNGQK+QDIATGKPSTPFDHGAGH
Sbjct: 541 SCPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 600

Query: 604 VDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARREFTCDAGKKYSVNDLNYPSF 663
           VDPVSALNPGLVYDLTVDDYL+FLCALNYTSSQINSLARR++TCD+GKKYSVN+LNYPSF
Sbjct: 601 VDPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSF 660

Query: 664 AAVFEGALGG---GSGVVKHTRTLTNVGSPGTYKVSITSETKLVKISVEPESLTFAEANE 723
           A VF+G LGG   GS VVKHTRTLTNVGSPGTYKVSI+SETKLVKISVEPESL+F +ANE
Sbjct: 661 AVVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 720

Query: 724 KKSYTATFATTTGSAAAPSTEGFGRIEWSDGKHVVGSPIAFSWT 762
           KKSYT TF T TGS+A PS EGFGRIEW+DGKHVVGSPIAFSWT
Sbjct: 721 KKSYTVTFTTATGSSAPPSAEGFGRIEWTDGKHVVGSPIAFSWT 764

BLAST of Sgr026245 vs. NCBI nr
Match: XP_023543760.1 (subtilisin-like protease SBT1.7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1401.0 bits (3625), Expect = 0.0e+00
Identity = 696/763 (91.22%), Postives = 735/763 (96.33%), Query Frame = 0

Query: 4   MAKPVRIFVLLCFVFG--VSMAAAEKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTA 63
           M KPV +F+L CF  G  ++ AAA+KKTY+VHMAKYQMP++FEHHLHWYD+SLKSVSDTA
Sbjct: 1   MEKPVWVFLLFCFFSGPFMAAAAAQKKTYVVHMAKYQMPQTFEHHLHWYDASLKSVSDTA 60

Query: 64  EMIYAYNNVVHGFSTRLTAEEAQRLEGQPGILAVVPEMRYELHTTRTPQFLGLDKNADLY 123
           +MIYAY  VVHGFSTRLTAEEAQRLE QPG+LAVVPEMRYELHTTRTPQFLGLDKNADLY
Sbjct: 61  DMIYAYKTVVHGFSTRLTAEEAQRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADLY 120

Query: 124 PESNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGAR 183
           PES S SEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGAR
Sbjct: 121 PESTSTSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGAR 180

Query: 184 FFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAAR 243
           FFS+GYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAAR
Sbjct: 181 FFSRGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAAR 240

Query: 244 ARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEK 303
           ARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGG+SDYYKDSVA+GAFAAMEK
Sbjct: 241 ARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEK 300

Query: 304 GILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAFVSLGDGKNFSGVSLYRGKPL 363
           GILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPA+VS+GDGKNFSGVSLYRGK L
Sbjct: 301 GILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKSL 360

Query: 364 PGTLLPFIYAANASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIG 423
           PGTLLPFIYAANASN++NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGIG
Sbjct: 361 PGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGIG 420

Query: 424 MVLANTAANGEELVADAHLLPATAVGQKSGDMIRKYLISEPNPTVTILFEGTKLGIEPSP 483
           MVLANTAANGEELVAD+HLLPA+AVGQKSGD+IRKYLIS+P PTVTILFEGTKLGIEPSP
Sbjct: 421 MVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPSP 480

Query: 484 VVAAFSSRGPNSITPQLLKPDIIAPGVNIIAGWSKSVGPSGLPIDDRRVDFNIISGTSMS 543
           VVAAFSSRGPNSITPQ+LKPDIIAPGVNI+AGWSK+VGPSGLPID+RRVDFNIISGTSMS
Sbjct: 481 VVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLPIDERRVDFNIISGTSMS 540

Query: 544 CPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKLQDIATGKPSTPFDHGAGHV 603
           CPHVSGLAALIKGAH DWSPAAIRSALMTTAYTAYKNGQK+QDIATGKPSTPFDHGAGHV
Sbjct: 541 CPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHV 600

Query: 604 DPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARREFTCDAGKKYSVNDLNYPSFA 663
           DPVSALNPGLVYDLTVDDYL+FLCALNYTSSQINSLARR++TCD+GKKYSVN+LNYPSFA
Sbjct: 601 DPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSFA 660

Query: 664 AVFEGALGG---GSGVVKHTRTLTNVGSPGTYKVSITSETKLVKISVEPESLTFAEANEK 723
            VF+G LGG   GS VVKHTRTLTNVGSPGTYKVSI+SETKLVKISVEPESL+F +ANEK
Sbjct: 661 VVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANEK 720

Query: 724 KSYTATFATTTGSAAAPSTEGFGRIEWSDGKHVVGSPIAFSWT 762
           KSYT TF T TGS+A PS EGFGRIEW+DGKHVVGSPIAFSWT
Sbjct: 721 KSYTVTFTTATGSSAPPSAEGFGRIEWTDGKHVVGSPIAFSWT 763

BLAST of Sgr026245 vs. NCBI nr
Match: KAG7034309.1 (Subtilisin-like protease SBT1.7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1401.0 bits (3625), Expect = 0.0e+00
Identity = 698/764 (91.36%), Postives = 734/764 (96.07%), Query Frame = 0

Query: 4   MAKPVRIFVLLCFVFGVSMA---AAEKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDT 63
           M KPV IF+L CF  G  MA   AA+KKTY+VHMAKYQMP++FEHHLHWYD+SLKSVSDT
Sbjct: 1   MEKPVWIFLLFCFFSGPFMAAAVAAQKKTYVVHMAKYQMPQTFEHHLHWYDASLKSVSDT 60

Query: 64  AEMIYAYNNVVHGFSTRLTAEEAQRLEGQPGILAVVPEMRYELHTTRTPQFLGLDKNADL 123
           A+MIYAY  VVHGFSTRLTAEEAQRLE QPG+LAVVPEMRYELHTTRTPQFLGLDKNADL
Sbjct: 61  ADMIYAYKTVVHGFSTRLTAEEAQRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADL 120

Query: 124 YPESNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 183
           YPES S SEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA
Sbjct: 121 YPESTSTSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180

Query: 184 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 243
           RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA
Sbjct: 181 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240

Query: 244 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAME 303
           RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGG+SDYYKDSVA+G+FAAME
Sbjct: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGSFAAME 300

Query: 304 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAFVSLGDGKNFSGVSLYRGKP 363
           KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPA+VS+GDGKNFSGVSLYRGK 
Sbjct: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKS 360

Query: 364 LPGTLLPFIYAANASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGI 423
           LPGTLLPFIYAANASN++NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGI
Sbjct: 361 LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGI 420

Query: 424 GMVLANTAANGEELVADAHLLPATAVGQKSGDMIRKYLISEPNPTVTILFEGTKLGIEPS 483
           GMVLANTAANGEELVAD+HLLPA+AVGQKSGD+IRKYLIS+P PTVTILFEGTKLGIEPS
Sbjct: 421 GMVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPS 480

Query: 484 PVVAAFSSRGPNSITPQLLKPDIIAPGVNIIAGWSKSVGPSGLPIDDRRVDFNIISGTSM 543
           PVVAAFSSRGPNSITPQ+LKPDIIAPGVNI+AGWSK+VGPSGLPID+RRVDFNIISGTSM
Sbjct: 481 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLPIDERRVDFNIISGTSM 540

Query: 544 SCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKLQDIATGKPSTPFDHGAGH 603
           SCPHVSGLAALIKGAH DWSPAAIRSALMTTAYTAYKNGQK+QDIATGKPSTPFDHGAGH
Sbjct: 541 SCPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 600

Query: 604 VDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARREFTCDAGKKYSVNDLNYPSF 663
           VDPVSALNPGLVYDLTVDDYL+FLCALNYTSSQINSLARR++TCD+GKKYSVN+LNYPSF
Sbjct: 601 VDPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSF 660

Query: 664 AAVFEGALGG---GSGVVKHTRTLTNVGSPGTYKVSITSETKLVKISVEPESLTFAEANE 723
           A VF+G LGG   GS VVKHTRTLTNVGSPGTYKVSI+SETKLVKISVEPESL+F +ANE
Sbjct: 661 AVVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 720

Query: 724 KKSYTATFATTTGSAAAPSTEGFGRIEWSDGKHVVGSPIAFSWT 762
           KKSYT TF T TGS+A PS EGFGRIEW+DGKHVVGSPIAFSWT
Sbjct: 721 KKSYTVTFTTATGSSAPPSAEGFGRIEWTDGKHVVGSPIAFSWT 764

BLAST of Sgr026245 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 1119.8 bits (2895), Expect = 0.0e+00
Identity = 550/753 (73.04%), Postives = 636/753 (84.46%), Query Frame = 0

Query: 11  FVLLCFVF-GVSMAAAEKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTAEMIYAYNN 70
           F+LLC  F  VS +++++ TYIVHMAK QMP SF+ H +WYDSSL+S+SD+AE++Y Y N
Sbjct: 12  FLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAELLYTYEN 71

Query: 71  VVHGFSTRLTAEEAQRLEGQPGILAVVPEMRYELHTTRTPQFLGLDKN-ADLYPESNSVS 130
            +HGFSTRLT EEA  L  QPG+++V+PE RYELHTTRTP FLGLD++ ADL+PE+ S S
Sbjct: 72  AIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYS 131

Query: 131 EVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARFFSKGYE 190
           +V++GVLDTGVWPESKS+ D G GP+PSSWKG CE+GTNF+AS CNRKLIGARFF++GYE
Sbjct: 132 DVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYE 191

Query: 191 ATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARARVAAYK 250
           +T+GPIDESKESRSPRDDDGHGTHT++TAAGSVVE ASL GYASGTARGMA RARVA YK
Sbjct: 192 STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYK 251

Query: 251 VCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKGILISCS 310
           VCW GGCFSSDILAAI+KA+ DNVNVLSMSLGGG+SDYY+D VA GAFAAME+GIL+SCS
Sbjct: 252 VCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCS 311

Query: 311 AGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAFVSLGDGKNFSGVSLYRGKPLPGTLLPF 370
           AGNAGPS  SLSN +PWITTVGAGTLDRDFPA   LG+GKNF+GVSL++G+ LP  LLPF
Sbjct: 312 AGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPF 371

Query: 371 IYAANASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVLANTA 430
           IYA NASNA+NGNLCMTGTLIPEKV GKIV CDRG+N RVQKG VVKAAGG+GM+LANTA
Sbjct: 372 IYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTA 431

Query: 431 ANGEELVADAHLLPATAVGQKSGDMIRKYLISEPNPTVTILFEGTKLGIEPSPVVAAFSS 490
           ANGEELVADAHLLPAT VG+K+GD+IR Y+ ++PNPT +I   GT +G++PSPVVAAFSS
Sbjct: 432 ANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSS 491

Query: 491 RGPNSITPQLLKPDIIAPGVNIIAGWSKSVGPSGLPIDDRRVDFNIISGTSMSCPHVSGL 550
           RGPNSITP +LKPD+IAPGVNI+A W+ + GP+GL  D RRV+FNIISGTSMSCPHVSGL
Sbjct: 492 RGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGL 551

Query: 551 AALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKLQDIATGKPSTPFDHGAGHVDPVSALN 610
           AAL+K  HP+WSPAAIRSALMTTAY  YK+G+ L DIATGKPSTPFDHGAGHV P +A N
Sbjct: 552 AALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATN 611

Query: 611 PGLVYDLTVDDYLNFLCALNYTSSQINSLARREFTCDAGKKYSVNDLNYPSFAAVFEGAL 670
           PGL+YDLT +DYL FLCALNYTS QI S++RR +TCD  K YSV DLNYPSFA   +G  
Sbjct: 612 PGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGV- 671

Query: 671 GGGSGVVKHTRTLTNVGSPGTYKVSITSETKLVKISVEPESLTFAEANEKKSYTATFATT 730
               G  K+TRT+T+VG  GTY V +TSET  VKISVEP  L F EANEKKSYT TF  T
Sbjct: 672 ----GAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTF--T 731

Query: 731 TGSAAAPSTEGFGRIEWSDGKHVVGSPIAFSWT 762
             S+    +  FG IEWSDGKHVVGSP+A SWT
Sbjct: 732 VDSSKPSGSNSFGSIEWSDGKHVVGSPVAISWT 757

BLAST of Sgr026245 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 808.1 bits (2086), Expect = 8.3e-233
Identity = 415/755 (54.97%), Postives = 542/755 (71.79%), Query Frame = 0

Query: 10  IFVLLCFVFGVSMAAAEKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTAEMIYAYNN 69
           I ++  F+F + +    KKTYI+ +     PESF  H  WY S L S S    ++Y Y  
Sbjct: 11  ITIITTFLF-LLLHTTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNSES---SLLYTYTT 70

Query: 70  VVHGFSTRLTAEEAQR-LEGQPGILAVVPEMRYELHTTRTPQFLGLDKNADLYPESNSVS 129
             HGFS  L + EA   L     IL +  +  Y LHTTRTP+FLGL+    ++   +S +
Sbjct: 71  SFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSN 130

Query: 130 EVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARFFSKGYE 189
            VIIGVLDTGVWPES+SFDDT +  +PS WKGECESG++F +  CN+KLIGAR FSKG++
Sbjct: 131 GVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQ 190

Query: 190 -ATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARARVAAY 249
            A+ G     +ES SPRD DGHGTHT+TTAAGS V NAS  GYA+GTARGMA RARVA Y
Sbjct: 191 MASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATY 250

Query: 250 KVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKGILISC 309
           KVCW+ GCF SDILAA+++A+ D V+VLS+SLGGG + YY+D++A GAF+AME+G+ +SC
Sbjct: 251 KVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSC 310

Query: 310 SAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAFVSLGDGKNFSGVSLYRGKPLPGTLLP 369
           SAGN+GP+  S++N +PW+ TVGAGTLDRDFPAF +LG+GK  +GVSLY G  +    L 
Sbjct: 311 SAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLE 370

Query: 370 FIYAANASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVLANT 429
            +Y  N  N+S+ NLC+ G+L    V GKIV CDRGVN RV+KGAVV+ AGG+GM++ANT
Sbjct: 371 LVY--NKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANT 430

Query: 430 AANGEELVADAHLLPATAVGQKSGDMIRKYLISEPNPTVTILFEGTKLGIEPSPVVAAFS 489
           AA+GEELVAD+HLLPA AVG+K+GD++R+Y+ S+  PT  ++F+GT L ++PSPVVAAFS
Sbjct: 431 AASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFS 490

Query: 490 SRGPNSITPQLLKPDIIAPGVNIIAGWSKSVGPSGLPIDDRRVDFNIISGTSMSCPHVSG 549
           SRGPN++TP++LKPD+I PGVNI+AGWS ++GP+GL  D RR  FNI+SGTSMSCPH+SG
Sbjct: 491 SRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISG 550

Query: 550 LAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKLQDIATGKPSTPFDHGAGHVDPVSAL 609
           LA L+K AHP+WSP+AI+SALMTTAY        L D A    S P+ HG+GHVDP  AL
Sbjct: 551 LAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKAL 610

Query: 610 NPGLVYDLTVDDYLNFLCALNYTSSQINSLARREFTCDAGKKYS-VNDLNYPSFAAVFEG 669
           +PGLVYD++ ++Y+ FLC+L+YT   I ++ +R  + +  KK+S    LNYPSF+ +F  
Sbjct: 611 SPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRP-SVNCSKKFSDPGQLNYPSFSVLF-- 670

Query: 670 ALGGGSGVVKHTRTLTNVGSPGT-YKVSITSETKLVKISVEPESLTFAEANEKKSYTATF 729
              GG  VV++TR +TNVG+  + YKV++      V ISV+P  L+F    EKK YT TF
Sbjct: 671 ---GGKRVVRYTREVTNVGAASSVYKVTVNGAPS-VGISVKPSKLSFKSVGEKKRYTVTF 730

Query: 730 ATTTGSAAAPSTEGFGRIEWSDGKHVVGSPIAFSW 761
            +  G +     E FG I WS+ +H V SP+AFSW
Sbjct: 731 VSKKGVSMTNKAE-FGSITWSNPQHEVRSPVAFSW 751

BLAST of Sgr026245 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 776.5 bits (2004), Expect = 2.7e-223
Identity = 396/750 (52.80%), Postives = 517/750 (68.93%), Query Frame = 0

Query: 27  KKTYIVHMAKYQMPESFEHHLHWYDSSLKSVS---------DTAEMIYAYNNVVHGFSTR 86
           KKTY++HM K  MP  + +HL WY S + SV+         +   ++Y Y    HG + +
Sbjct: 34  KKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNNRILYTYQTAFHGLAAQ 93

Query: 87  LTAEEAQRLEGQPGILAVVPEMRYELHTTRTPQFLGLDKNAD--LYPESNSVSEVIIGVL 146
           LT EEA+RLE + G++AV+PE RYELHTTR+P FLGL++     ++ E  +  +V++GVL
Sbjct: 94  LTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVWAERVTDHDVVVGVL 153

Query: 147 DTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARFFSKGYEATLGPID 206
           DTG+WPES+SF+DTG+ PVP++W+G CE+G  F   NCNRK++GAR F +GYEA  G ID
Sbjct: 154 DTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGARVFYRGYEAATGKID 213

Query: 207 ESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARARVAAYKVCWAGGC 266
           E  E +SPRD DGHGTHTA T AGS V+ A+LFG+A GTARGMA +ARVAAYKVCW GGC
Sbjct: 214 EELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVAAYKVCWVGGC 273

Query: 267 FSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKGILISCSAGNAGPS 326
           FSSDIL+A+++AV D V VLS+SLGGGVS Y +DS++   F AME G+ +SCSAGN GP 
Sbjct: 274 FSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPD 333

Query: 327 PYSLSNTSPWITTVGAGTLDRDFPAFVSLGDGKNFSGVSLYRGKPL--PGTLLPFIY-AA 386
           P SL+N SPWITTVGA T+DRDFPA V +G  + F GVSLY+G+ +       P +Y   
Sbjct: 334 PISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYLGR 393

Query: 387 NASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVLANTAANGE 446
           NAS+    + C+ G L    VAGKIV CDRGV PRVQKG VVK AGGIGMVL NTA NGE
Sbjct: 394 NASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGE 453

Query: 447 ELVADAHLLPATAVGQKSGDMIRKYLISEPNPTVTILFEGTKLGIEPSPVVAAFSSRGPN 506
           ELVAD+H+LPA AVG+K G +I++Y ++    T ++   GT++GI+PSPVVAAFSSRGPN
Sbjct: 454 ELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGIKPSPVVAAFSSRGPN 513

Query: 507 SITPQLLKPDIIAPGVNIIAGWSKSVGPSGLPIDDRRVDFNIISGTSMSCPHVSGLAALI 566
            ++ ++LKPD++APGVNI+A W+  + PS L  D RRV FNI+SGTSMSCPHVSG+AALI
Sbjct: 514 FLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGVAALI 573

Query: 567 KGAHPDWSPAAIRSALMTTAYTAYKNGQKLQDIATGKPSTPFDHGAGHVDPVSALNPGLV 626
           K  HPDWSPAAI+SALMTTAY      + L D +   PS+P+DHGAGH+DP+ A +PGLV
Sbjct: 574 KSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLV 633

Query: 627 YDLTVDDYLNFLCALNYTSSQINSLARR-EFTCDAGKKYSVNDLNYPSFAAVFEGALGGG 686
           YD+   +Y  FLC  + + SQ+    +    TC      +  +LNYP+ +A+F       
Sbjct: 634 YDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNYPAISALFPENTHVK 693

Query: 687 SGVVKHTRTLTNVGSP-GTYKVSITSETKLVKISVEPESLTFAEANEKKSYTATFATTTG 746
           +  ++  RT+TNVG    +YKVS+ S  K   ++V+P++L F   ++K SYT TF T   
Sbjct: 694 AMTLR--RTVTNVGPHISSYKVSV-SPFKGASVTVQPKTLNFTSKHQKLSYTVTFRT--- 753

Query: 747 SAAAPSTEGFGRIEWSDGKHVVGSPIAFSW 761
                    FG + W    H V SP+  +W
Sbjct: 754 -RFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Sgr026245 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 769.2 bits (1985), Expect = 4.3e-221
Identity = 402/764 (52.62%), Postives = 519/764 (67.93%), Query Frame = 0

Query: 12  VLLCFVFGVSMAAAEKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDT---AEMIYAYN 71
           +LLCF    S ++   ++YIVH+ +   P  F  H +W+ S L+S+  +   A ++Y+Y+
Sbjct: 15  LLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQPATLLYSYS 74

Query: 72  NVVHGFSTRLTAEEAQRLEGQPGILAVVPEMRYELHTTRTPQFLGLDKNADLYPESNSVS 131
             VHGFS RL+  +   L   P +++V+P+   E+HTT TP FLG  +N+ L+  SN   
Sbjct: 75  RAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYGE 134

Query: 132 EVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARFFSKGYE 191
           +VI+GVLDTG+WPE  SF D+GLGP+PS+WKGECE G +F AS+CNRKLIGAR F +GY 
Sbjct: 135 DVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYL 194

Query: 192 ATLGPIDE--SKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARARVAA 251
                  +  +KESRSPRD +GHGTHTA+TAAGSVV NASL+ YA GTA GMA++AR+AA
Sbjct: 195 TQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAA 254

Query: 252 YKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLG--GGVSDYYKDSVATGAFAAMEKGIL 311
           YK+CW GGC+ SDILAA+++AV D V+V+S+S+G  G   +Y+ DS+A GAF A   GI+
Sbjct: 255 YKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIV 314

Query: 312 ISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAFVSLGDGKNFSGVSLYRGKPLPGT 371
           +SCSAGN+GP+P + +N +PWI TVGA T+DR+F A    GDGK F+G SLY G+ LP +
Sbjct: 315 VSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDS 374

Query: 372 LLPFIYAANASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVL 431
            L  +Y+ +  +     LC  G L    V GKIV CDRG N RV+KG+ VK AGG GM+L
Sbjct: 375 QLSLVYSGDCGS----RLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGGAGMIL 434

Query: 432 ANTAANGEELVADAHLLPATAVGQKSGDMIRKYLISEPNPTVTILFEGTKLG-IEPSPVV 491
           ANTA +GEEL AD+HL+PAT VG K+GD IR Y+ +  +PT  I F GT +G   PSP V
Sbjct: 435 ANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSPRV 494

Query: 492 AAFSSRGPNSITPQLLKPDIIAPGVNIIAGWSKSVGPSGLPIDDRRVDFNIISGTSMSCP 551
           AAFSSRGPN +TP +LKPD+IAPGVNI+AGW+  VGP+ L ID RRV FNIISGTSMSCP
Sbjct: 495 AAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGTSMSCP 554

Query: 552 HVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKLQDIATGKPSTPFDHGAGHVDP 611
           HVSGLAAL++ AHPDWSPAAI+SAL+TTAY    +G+ ++D+ATGK S  F HGAGHVDP
Sbjct: 555 HVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHVDP 614

Query: 612 VSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARREF---TCDAGKKYSVNDLNYPSF 671
             ALNPGLVYD+ V +Y+ FLCA+ Y    I    +       C+  K  +  DLNYPSF
Sbjct: 615 NKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPSF 674

Query: 672 AAVFEGALGGGSGVVKHTRTLTNVGS--PGTYKVSITSETKLVKISVEPESLTFAEANEK 731
           + VF         VVK+ R + NVGS     Y+V + S    V+I V P  L F++    
Sbjct: 675 SVVFAST----GEVVKYKRVVKNVGSNVDAVYEVGVKSPAN-VEIDVSPSKLAFSKEKSV 734

Query: 732 KSYTATFATTTGSAAAPSTEG--FGRIEWSDGKHVVGSPIAFSW 761
             Y  TF +        S  G  FG IEW+DG+HVV SP+A  W
Sbjct: 735 LEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of Sgr026245 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 3.4e-218
Identity = 391/751 (52.06%), Postives = 516/751 (68.71%), Query Frame = 0

Query: 21  SMAAAEKKTYIVHMAKYQMPESFEHHLHWYDSSLKSV-SDTAEMIYAYNNVVHGFSTRLT 80
           S +++   TYIVH+     P  F  H HWY SSL S+ S    +I+ Y+ V HGFS RLT
Sbjct: 19  SASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSIIHTYDTVFHGFSARLT 78

Query: 81  AEEAQRLEGQPGILAVVPEMRYELHTTRTPQFLGL--DKNADLYPESNSVSEVIIGVLDT 140
           +++A +L   P +++V+PE    LHTTR+P+FLGL     A L  ES+  S+++IGV+DT
Sbjct: 79  SQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVIDT 138

Query: 141 GVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARFFSKGYEATLGPIDES 200
           GVWPE  SFDD GLGPVP  WKG+C +  +F  S CNRKL+GARFF  GYEAT G ++E+
Sbjct: 139 GVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNET 198

Query: 201 KESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARARVAAYKVCWAGGCFS 260
            E RSPRD DGHGTHTA+ +AG  V  AS  GYA G A GMA +AR+AAYKVCW  GC+ 
Sbjct: 199 TEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYD 258

Query: 261 SDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKGILISCSAGNAGPSPY 320
           SDILAA + AV D V+V+S+S+GG V  YY D++A GAF A+++GI +S SAGN GP   
Sbjct: 259 SDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGAL 318

Query: 321 SLSNTSPWITTVGAGTLDRDFPAFVSLGDGKNFSGVSLYRGKPL-PGTLLPFIYAAN--A 380
           +++N +PW+TTVGAGT+DRDFPA V LG+GK  SGVS+Y G  L PG + P +Y  +   
Sbjct: 319 TVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSLLG 378

Query: 381 SNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVLANTAANGEEL 440
            +  + +LC+ G+L P  V GKIV CDRG+N R  KG +V+  GG+GM++AN   +GE L
Sbjct: 379 GDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGL 438

Query: 441 VADAHLLPATAVGQKSGDMIRKYLI------SEPNPTVTILFEGTKLGIEPSPVVAAFSS 500
           VAD H+LPAT+VG   GD IR+Y+       S  +PT TI+F+GT+LGI P+PVVA+FS+
Sbjct: 439 VADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSA 498

Query: 501 RGPNSITPQLLKPDIIAPGVNIIAGWSKSVGPSGLPIDDRRVDFNIISGTSMSCPHVSGL 560
           RGPN  TP++LKPD+IAPG+NI+A W   +GPSG+  D+RR +FNI+SGTSM+CPHVSGL
Sbjct: 499 RGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGL 558

Query: 561 AALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKLQDIATGKPSTPFDHGAGHVDPVSALN 620
           AAL+K AHPDWSPAAIRSAL+TTAYT   +G+ + D +TG  S+  D+G+GHV P  A++
Sbjct: 559 AALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMD 618

Query: 621 PGLVYDLTVDDYLNFLCALNYTSSQINSLARREFTCDAGKKYS-VNDLNYPSFAAVFEGA 680
           PGLVYD+T  DY+NFLC  NYT + I ++ RR+  CD  ++   V +LNYPSF+ VF+  
Sbjct: 619 PGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQ-Q 678

Query: 681 LGGGSGVVKHTRTLTNVG-SPGTYKVSITSETKLVKISVEPESLTFAEANEKKSYTATFA 740
            G         RT+TNVG S   Y++ I    +   ++VEPE L+F    +K S+     
Sbjct: 679 YGESKMSTHFIRTVTNVGDSDSVYEIKI-RPPRGTTVTVEPEKLSFRRVGQKLSFVVRVK 738

Query: 741 TTTGSAAAPSTE-GFGRIEWSDGKHVVGSPI 757
           TT    +  +T    G I WSDGK  V SP+
Sbjct: 739 TTEVKLSPGATNVETGHIVWSDGKRNVTSPL 767

BLAST of Sgr026245 vs. ExPASy TrEMBL
Match: A0A6J1GEZ1 (subtilisin-like protease SBT1.7 OS=Cucurbita moschata OX=3662 GN=LOC111453585 PE=3 SV=1)

HSP 1 Score: 1402.9 bits (3630), Expect = 0.0e+00
Identity = 699/764 (91.49%), Postives = 735/764 (96.20%), Query Frame = 0

Query: 4   MAKPVRIFVLLCFVFGVSMA---AAEKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDT 63
           M KPV IF+L CF  G  MA   AA+KKTY+VHMAKYQMP++FEHHLHWYD+SLKSVSDT
Sbjct: 1   MVKPVWIFLLFCFFSGPFMAAAVAAQKKTYVVHMAKYQMPQTFEHHLHWYDASLKSVSDT 60

Query: 64  AEMIYAYNNVVHGFSTRLTAEEAQRLEGQPGILAVVPEMRYELHTTRTPQFLGLDKNADL 123
           A+MIYAY  VVHGFSTRLTAEEAQRLE QPG+LAVVPEMRYELHTTRTPQFLGLDKNADL
Sbjct: 61  ADMIYAYKTVVHGFSTRLTAEEAQRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADL 120

Query: 124 YPESNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 183
           YPES S SEVIIGVLDTGVWPESKSFDD+GLGPVPSSWKGECESGTNFSASNCNRKLIGA
Sbjct: 121 YPESTSTSEVIIGVLDTGVWPESKSFDDSGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180

Query: 184 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 243
           RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA
Sbjct: 181 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240

Query: 244 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAME 303
           RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGG+SDYYKDSVA+GAFAAME
Sbjct: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300

Query: 304 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAFVSLGDGKNFSGVSLYRGKP 363
           KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPA+VS+GDGKNFSGVSLYRGK 
Sbjct: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKS 360

Query: 364 LPGTLLPFIYAANASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGI 423
           LPGTLLPFIYAANASN++NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGI
Sbjct: 361 LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGI 420

Query: 424 GMVLANTAANGEELVADAHLLPATAVGQKSGDMIRKYLISEPNPTVTILFEGTKLGIEPS 483
           GMVLANTAANGEELVAD+HLLPA+AVGQKSGD+IRKYLIS+P PTVTILFEGTKLGIEPS
Sbjct: 421 GMVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPS 480

Query: 484 PVVAAFSSRGPNSITPQLLKPDIIAPGVNIIAGWSKSVGPSGLPIDDRRVDFNIISGTSM 543
           PVVAAFSSRGPNSITPQ+LKPDIIAPGVNI+AGWSK+VGPSGLPID+RRVDFNIISGTSM
Sbjct: 481 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLPIDERRVDFNIISGTSM 540

Query: 544 SCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKLQDIATGKPSTPFDHGAGH 603
           SCPHVSGLAALIKGAH DWSPAAIRSALMTTAYTAYKNGQK+QDIATGKPSTPFDHGAGH
Sbjct: 541 SCPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 600

Query: 604 VDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARREFTCDAGKKYSVNDLNYPSF 663
           VDPVSALNPGLVYDLTVDDYL+FLCALNYTSSQINSLARR++TCD+GKKYSVN+LNYPSF
Sbjct: 601 VDPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSF 660

Query: 664 AAVFEGALGG---GSGVVKHTRTLTNVGSPGTYKVSITSETKLVKISVEPESLTFAEANE 723
           A VF+G LGG   GS VVKHTRTLTNVGSPGTYKVSI+SETKLVKISVEPESL+F +ANE
Sbjct: 661 AVVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 720

Query: 724 KKSYTATFATTTGSAAAPSTEGFGRIEWSDGKHVVGSPIAFSWT 762
           KKSYT TF TTTGS+A PS EGFGRIEW+DGKHVVGSPIAFSWT
Sbjct: 721 KKSYTVTFTTTTGSSAPPSAEGFGRIEWTDGKHVVGSPIAFSWT 764

BLAST of Sgr026245 vs. ExPASy TrEMBL
Match: A0A6J1IIG3 (subtilisin-like protease SBT1.7 OS=Cucurbita maxima OX=3661 GN=LOC111477791 PE=3 SV=1)

HSP 1 Score: 1397.1 bits (3615), Expect = 0.0e+00
Identity = 697/764 (91.23%), Postives = 732/764 (95.81%), Query Frame = 0

Query: 4   MAKPVRIFVLLCFVFGVSM---AAAEKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDT 63
           M KPVRIF+L CF  G  M   AAA+KKTY+VHMAKYQMP +FEHHLHWYD+SLKSVSDT
Sbjct: 1   MEKPVRIFLLFCFFSGPFMAAAAAAQKKTYVVHMAKYQMPLTFEHHLHWYDASLKSVSDT 60

Query: 64  AEMIYAYNNVVHGFSTRLTAEEAQRLEGQPGILAVVPEMRYELHTTRTPQFLGLDKNADL 123
           A+MIYAY  VVHGFSTRLTAEEA+RLE QPG+LAVVPEMRYELHTTRTPQFLGLDKNADL
Sbjct: 61  ADMIYAYKTVVHGFSTRLTAEEAKRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADL 120

Query: 124 YPESNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 183
           YPES S SEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA
Sbjct: 121 YPESTSTSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180

Query: 184 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 243
           RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA
Sbjct: 181 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240

Query: 244 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAME 303
           RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGG+SDYYKDSVA+GAFAAME
Sbjct: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300

Query: 304 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAFVSLGDGKNFSGVSLYRGKP 363
           KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPA+VS+GDGKNFSGVSLYRGKP
Sbjct: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKP 360

Query: 364 LPGTLLPFIYAANASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGI 423
           L GTLLPFIYAANASN++NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGI
Sbjct: 361 LSGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGI 420

Query: 424 GMVLANTAANGEELVADAHLLPATAVGQKSGDMIRKYLISEPNPTVTILFEGTKLGIEPS 483
           GMVLANTAANGEELVAD+HLLPA+AVGQKSGD+IRKYLIS+P PTVTILFEGTKLGIEPS
Sbjct: 421 GMVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPS 480

Query: 484 PVVAAFSSRGPNSITPQLLKPDIIAPGVNIIAGWSKSVGPSGLPIDDRRVDFNIISGTSM 543
           PVVAAFSSRGPNSITPQ+LKPDIIAPGVNI+AGWSK+VGPSGL ID+RRVDFNIISGTSM
Sbjct: 481 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAIDERRVDFNIISGTSM 540

Query: 544 SCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKLQDIATGKPSTPFDHGAGH 603
           SCPHVSGLAALIKGAH DWSPAAIRSALMTTAYTAYKNGQK+QDIATGKPSTPFDHGAGH
Sbjct: 541 SCPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 600

Query: 604 VDPVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARREFTCDAGKKYSVNDLNYPSF 663
           VDPVSALNPGLVYDLTVDDYL+FLCALNYTSSQINSLARR++TCD+GKKYSVN+LNYPSF
Sbjct: 601 VDPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSF 660

Query: 664 AAVFEGALGG---GSGVVKHTRTLTNVGSPGTYKVSITSETKLVKISVEPESLTFAEANE 723
           A VF+G LGG   GS VVKHTRTLTNVGSPGTYKVSI+SETKLVKISVEPESL+F +ANE
Sbjct: 661 AVVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 720

Query: 724 KKSYTATFATTTGSAAAPSTEGFGRIEWSDGKHVVGSPIAFSWT 762
           KKSYT TF T TGS+  PS EGFGRIEW+DGKHVVGSPIAFSWT
Sbjct: 721 KKSYTVTFTTATGSSVPPSAEGFGRIEWTDGKHVVGSPIAFSWT 764

BLAST of Sgr026245 vs. ExPASy TrEMBL
Match: A0A0A0KIG4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G493920 PE=3 SV=1)

HSP 1 Score: 1392.9 bits (3604), Expect = 0.0e+00
Identity = 697/761 (91.59%), Postives = 727/761 (95.53%), Query Frame = 0

Query: 4   MAKPVRIFVLLCFVFGVSMAAAEKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTAEM 63
           MA PV +F+LLCF    SMA  +KKTYIVHMAKYQMPESFEHHLHWYDSSL+SVSD+AEM
Sbjct: 1   MANPVWMFLLLCFFSVPSMAVGDKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDSAEM 60

Query: 64  IYAYNNVVHGFSTRLTAEEAQRLEGQPGILAVVPEMRYELHTTRTPQFLGLDKNADLYPE 123
           IYAYNNVVHGFSTRLTAEEAQRLE QPGILAVVPEM YELHTTR+P+FLGLDKNA+LYPE
Sbjct: 61  IYAYNNVVHGFSTRLTAEEAQRLEAQPGILAVVPEMIYELHTTRSPEFLGLDKNANLYPE 120

Query: 124 SNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARFF 183
           SNSVSEVIIGVLDTG+ PESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKL+GARFF
Sbjct: 121 SNSVSEVIIGVLDTGISPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLVGARFF 180

Query: 184 SKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARAR 243
           SKGYEATLGPIDESKESRSPRDDDGHGTHTA+TAAGSVVENASLFGYASGTARGMAARAR
Sbjct: 181 SKGYEATLGPIDESKESRSPRDDDGHGTHTASTAAGSVVENASLFGYASGTARGMAARAR 240

Query: 244 VAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKGI 303
           VAAYKVCWAGGCFSSDI+AAI+KAV+DNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKGI
Sbjct: 241 VAAYKVCWAGGCFSSDIVAAIDKAVDDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKGI 300

Query: 304 LISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAFVSLGDGKNFSGVSLYRGKPLPG 363
           L+SCSAGNAGPSP+SLSNTSPWITTVGAGTLDRDFPA+VSLGD KNFSGVSLYRGK LPG
Sbjct: 301 LVSCSAGNAGPSPFSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKSLPG 360

Query: 364 TLLPFIYAANASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMV 423
           TLLPFIYAANASN+ NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGIGMV
Sbjct: 361 TLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGIGMV 420

Query: 424 LANTAANGEELVADAHLLPATAVGQKSGDMIRKYLISEPNPTVTILFEGTKLGIEPSPVV 483
           LANTAANGEELVAD+HLLPATAVGQKSGD IRKYL+S+P+PTVTILFEGTKLGIEPSPVV
Sbjct: 421 LANTAANGEELVADSHLLPATAVGQKSGDTIRKYLVSDPSPTVTILFEGTKLGIEPSPVV 480

Query: 484 AAFSSRGPNSITPQLLKPDIIAPGVNIIAGWSKSVGPSGLPIDDRRVDFNIISGTSMSCP 543
           AAFSSRGPNSITPQLLKPDIIAPGVNI+AGWSKSVGPSGL IDDRRVDFNIISGTSMSCP
Sbjct: 481 AAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSCP 540

Query: 544 HVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKLQDIATGKPSTPFDHGAGHVDP 603
           HVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQK+QDIATGKPSTPFDHGAGHVDP
Sbjct: 541 HVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVDP 600

Query: 604 VSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARREFTCDAGKKYSVNDLNYPSFAAV 663
           VSALNPGLVYDLTVDDYLNFLCALNYT SQINSLAR++FTCD+ KKYSVNDLNYPSFA V
Sbjct: 601 VSALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARKDFTCDSKKKYSVNDLNYPSFAVV 660

Query: 664 FEGALGG---GSGVVKHTRTLTNVGSPGTYKVSITSETKLVKISVEPESLTFAEANEKKS 723
           FEG LGG   GS VVKHTRTLTNVGSPGTYKVSITSETK VKISVEPESL+F  AN+KKS
Sbjct: 661 FEGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSITSETKSVKISVEPESLSFTGANDKKS 720

Query: 724 YTATFATTTGSAAAPSTEGFGRIEWSDGKHVVGSPIAFSWT 762
           YT TF TTT SAA  S E FGRIEWSDGKHVVGSPIAFSWT
Sbjct: 721 YTVTFTTTTSSAAPTSAEAFGRIEWSDGKHVVGSPIAFSWT 761

BLAST of Sgr026245 vs. ExPASy TrEMBL
Match: A0A5D3CR08 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004280 PE=3 SV=1)

HSP 1 Score: 1389.0 bits (3594), Expect = 0.0e+00
Identity = 698/762 (91.60%), Postives = 727/762 (95.41%), Query Frame = 0

Query: 4   MAKPVRIFVLLCFVFGVSMAAAE-KKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTAE 63
           MA PV  F+LLC     SMA  + KKTYIVHMAKYQMPESFEHHLHWYDSSL+SVSD+AE
Sbjct: 1   MANPVWTFLLLCLFSVPSMAVGDKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDSAE 60

Query: 64  MIYAYNNVVHGFSTRLTAEEAQRLEGQPGILAVVPEMRYELHTTRTPQFLGLDKNADLYP 123
           MIYAYNNVVHGFSTRLT EEAQRLE QPGILAVVPEMRYELHTTR+PQFLGLDKNA+LYP
Sbjct: 61  MIYAYNNVVHGFSTRLTTEEAQRLEAQPGILAVVPEMRYELHTTRSPQFLGLDKNANLYP 120

Query: 124 ESNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF 183
           ESNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF
Sbjct: 121 ESNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF 180

Query: 184 FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARA 243
           FSKGYEATLGPIDESKESRSPRDDDGHGTHTA+TAAGSVVENASLFGYASGTARGMAARA
Sbjct: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTASTAAGSVVENASLFGYASGTARGMAARA 240

Query: 244 RVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKG 303
           RVAAYKVCWAGGCFSSDI+AA++KAVEDNVNV+SMSLGGGVSDYYKDSVATGAFAAMEKG
Sbjct: 241 RVAAYKVCWAGGCFSSDIVAAMDKAVEDNVNVMSMSLGGGVSDYYKDSVATGAFAAMEKG 300

Query: 304 ILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAFVSLGDGKNFSGVSLYRGKPLP 363
           IL+SCSAGNAGPSP+SLSNTSPWITTVGAGTLDRDFPA+VSLGD KNFSGVSLYRGK LP
Sbjct: 301 ILVSCSAGNAGPSPFSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKSLP 360

Query: 364 GTLLPFIYAANASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGM 423
           GTLLPFIYAANASN+ NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGIGM
Sbjct: 361 GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGIGM 420

Query: 424 VLANTAANGEELVADAHLLPATAVGQKSGDMIRKYLISEPNPTVTILFEGTKLGIEPSPV 483
           VLANTAANGEELVAD+HLLPATAVGQKSGD+IRKYL+S+P PTVTILFEGTKLGIEPSPV
Sbjct: 421 VLANTAANGEELVADSHLLPATAVGQKSGDIIRKYLVSDPKPTVTILFEGTKLGIEPSPV 480

Query: 484 VAAFSSRGPNSITPQLLKPDIIAPGVNIIAGWSKSVGPSGLPIDDRRVDFNIISGTSMSC 543
           VAAFSSRGPNSITPQLLKPDIIAPGVNI+AGWSKSVGPSGL IDDRRVDFNIISGTSMSC
Sbjct: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540

Query: 544 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKLQDIATGKPSTPFDHGAGHVD 603
           PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQK+QDIATGKPSTPFDHGAGHVD
Sbjct: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVD 600

Query: 604 PVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARREFTCDAGKKYSVNDLNYPSFAA 663
           PVSALNPGLVYDLTVDDYLNFLCALNYT SQINSLAR++FTCD+ KKYSVNDLNYPSFA 
Sbjct: 601 PVSALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARKDFTCDSKKKYSVNDLNYPSFAV 660

Query: 664 VFEGALGG---GSGVVKHTRTLTNVGSPGTYKVSITSETKLVKISVEPESLTFAEANEKK 723
           VF+G LGG   GS VVKHTRTLTNVGSPGTYKVSI+SETK VKISVEPESL+F  ANEKK
Sbjct: 661 VFDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKSVKISVEPESLSFTGANEKK 720

Query: 724 SYTATFATTTGSAAAPSTEGFGRIEWSDGKHVVGSPIAFSWT 762
           SYT TF T+ GSAA  S EGFGRIEWSDGKHVVGSPIAFSWT
Sbjct: 721 SYTVTF-TSNGSAAPSSAEGFGRIEWSDGKHVVGSPIAFSWT 761

BLAST of Sgr026245 vs. ExPASy TrEMBL
Match: A0A1S3B0Y2 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103484826 PE=3 SV=1)

HSP 1 Score: 1389.0 bits (3594), Expect = 0.0e+00
Identity = 698/762 (91.60%), Postives = 727/762 (95.41%), Query Frame = 0

Query: 4   MAKPVRIFVLLCFVFGVSMAAAE-KKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTAE 63
           MA PV  F+LLC     SMA  + KKTYIVHMAKYQMPESFEHHLHWYDSSL+SVSD+AE
Sbjct: 1   MANPVWTFLLLCLFSVPSMAVGDKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDSAE 60

Query: 64  MIYAYNNVVHGFSTRLTAEEAQRLEGQPGILAVVPEMRYELHTTRTPQFLGLDKNADLYP 123
           MIYAYNNVVHGFSTRLT EEAQRLE QPGILAVVPEMRYELHTTR+PQFLGLDKNA+LYP
Sbjct: 61  MIYAYNNVVHGFSTRLTTEEAQRLEAQPGILAVVPEMRYELHTTRSPQFLGLDKNANLYP 120

Query: 124 ESNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF 183
           ESNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF
Sbjct: 121 ESNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF 180

Query: 184 FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARA 243
           FSKGYEATLGPIDESKESRSPRDDDGHGTHTA+TAAGSVVENASLFGYASGTARGMAARA
Sbjct: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTASTAAGSVVENASLFGYASGTARGMAARA 240

Query: 244 RVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKG 303
           RVAAYKVCWAGGCFSSDI+AA++KAVEDNVNV+SMSLGGGVSDYYKDSVATGAFAAMEKG
Sbjct: 241 RVAAYKVCWAGGCFSSDIVAAMDKAVEDNVNVMSMSLGGGVSDYYKDSVATGAFAAMEKG 300

Query: 304 ILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAFVSLGDGKNFSGVSLYRGKPLP 363
           IL+SCSAGNAGPSP+SLSNTSPWITTVGAGTLDRDFPA+VSLGD KNFSGVSLYRGK LP
Sbjct: 301 ILVSCSAGNAGPSPFSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKSLP 360

Query: 364 GTLLPFIYAANASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGM 423
           GTLLPFIYAANASN+ NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGIGM
Sbjct: 361 GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGIGM 420

Query: 424 VLANTAANGEELVADAHLLPATAVGQKSGDMIRKYLISEPNPTVTILFEGTKLGIEPSPV 483
           VLANTAANGEELVAD+HLLPATAVGQKSGD+IRKYL+S+P PTVTILFEGTKLGIEPSPV
Sbjct: 421 VLANTAANGEELVADSHLLPATAVGQKSGDIIRKYLVSDPKPTVTILFEGTKLGIEPSPV 480

Query: 484 VAAFSSRGPNSITPQLLKPDIIAPGVNIIAGWSKSVGPSGLPIDDRRVDFNIISGTSMSC 543
           VAAFSSRGPNSITPQLLKPDIIAPGVNI+AGWSKSVGPSGL IDDRRVDFNIISGTSMSC
Sbjct: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540

Query: 544 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKLQDIATGKPSTPFDHGAGHVD 603
           PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQK+QDIATGKPSTPFDHGAGHVD
Sbjct: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVD 600

Query: 604 PVSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARREFTCDAGKKYSVNDLNYPSFAA 663
           PVSALNPGLVYDLTVDDYLNFLCALNYT SQINSLAR++FTCD+ KKYSVNDLNYPSFA 
Sbjct: 601 PVSALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARKDFTCDSKKKYSVNDLNYPSFAV 660

Query: 664 VFEGALGG---GSGVVKHTRTLTNVGSPGTYKVSITSETKLVKISVEPESLTFAEANEKK 723
           VF+G LGG   GS VVKHTRTLTNVGSPGTYKVSI+SETK VKISVEPESL+F  ANEKK
Sbjct: 661 VFDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKSVKISVEPESLSFTGANEKK 720

Query: 724 SYTATFATTTGSAAAPSTEGFGRIEWSDGKHVVGSPIAFSWT 762
           SYT TF T+ GSAA  S EGFGRIEWSDGKHVVGSPIAFSWT
Sbjct: 721 SYTVTF-TSNGSAAPSSAEGFGRIEWSDGKHVVGSPIAFSWT 761

BLAST of Sgr026245 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 1119.8 bits (2895), Expect = 0.0e+00
Identity = 550/753 (73.04%), Postives = 636/753 (84.46%), Query Frame = 0

Query: 11  FVLLCFVF-GVSMAAAEKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTAEMIYAYNN 70
           F+LLC  F  VS +++++ TYIVHMAK QMP SF+ H +WYDSSL+S+SD+AE++Y Y N
Sbjct: 12  FLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAELLYTYEN 71

Query: 71  VVHGFSTRLTAEEAQRLEGQPGILAVVPEMRYELHTTRTPQFLGLDKN-ADLYPESNSVS 130
            +HGFSTRLT EEA  L  QPG+++V+PE RYELHTTRTP FLGLD++ ADL+PE+ S S
Sbjct: 72  AIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYS 131

Query: 131 EVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARFFSKGYE 190
           +V++GVLDTGVWPESKS+ D G GP+PSSWKG CE+GTNF+AS CNRKLIGARFF++GYE
Sbjct: 132 DVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYE 191

Query: 191 ATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARARVAAYK 250
           +T+GPIDESKESRSPRDDDGHGTHT++TAAGSVVE ASL GYASGTARGMA RARVA YK
Sbjct: 192 STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYK 251

Query: 251 VCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKGILISCS 310
           VCW GGCFSSDILAAI+KA+ DNVNVLSMSLGGG+SDYY+D VA GAFAAME+GIL+SCS
Sbjct: 252 VCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCS 311

Query: 311 AGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAFVSLGDGKNFSGVSLYRGKPLPGTLLPF 370
           AGNAGPS  SLSN +PWITTVGAGTLDRDFPA   LG+GKNF+GVSL++G+ LP  LLPF
Sbjct: 312 AGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPF 371

Query: 371 IYAANASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVLANTA 430
           IYA NASNA+NGNLCMTGTLIPEKV GKIV CDRG+N RVQKG VVKAAGG+GM+LANTA
Sbjct: 372 IYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTA 431

Query: 431 ANGEELVADAHLLPATAVGQKSGDMIRKYLISEPNPTVTILFEGTKLGIEPSPVVAAFSS 490
           ANGEELVADAHLLPAT VG+K+GD+IR Y+ ++PNPT +I   GT +G++PSPVVAAFSS
Sbjct: 432 ANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSS 491

Query: 491 RGPNSITPQLLKPDIIAPGVNIIAGWSKSVGPSGLPIDDRRVDFNIISGTSMSCPHVSGL 550
           RGPNSITP +LKPD+IAPGVNI+A W+ + GP+GL  D RRV+FNIISGTSMSCPHVSGL
Sbjct: 492 RGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGL 551

Query: 551 AALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKLQDIATGKPSTPFDHGAGHVDPVSALN 610
           AAL+K  HP+WSPAAIRSALMTTAY  YK+G+ L DIATGKPSTPFDHGAGHV P +A N
Sbjct: 552 AALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATN 611

Query: 611 PGLVYDLTVDDYLNFLCALNYTSSQINSLARREFTCDAGKKYSVNDLNYPSFAAVFEGAL 670
           PGL+YDLT +DYL FLCALNYTS QI S++RR +TCD  K YSV DLNYPSFA   +G  
Sbjct: 612 PGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGV- 671

Query: 671 GGGSGVVKHTRTLTNVGSPGTYKVSITSETKLVKISVEPESLTFAEANEKKSYTATFATT 730
               G  K+TRT+T+VG  GTY V +TSET  VKISVEP  L F EANEKKSYT TF  T
Sbjct: 672 ----GAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTF--T 731

Query: 731 TGSAAAPSTEGFGRIEWSDGKHVVGSPIAFSWT 762
             S+    +  FG IEWSDGKHVVGSP+A SWT
Sbjct: 732 VDSSKPSGSNSFGSIEWSDGKHVVGSPVAISWT 757

BLAST of Sgr026245 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 808.1 bits (2086), Expect = 5.9e-234
Identity = 415/755 (54.97%), Postives = 542/755 (71.79%), Query Frame = 0

Query: 10  IFVLLCFVFGVSMAAAEKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDTAEMIYAYNN 69
           I ++  F+F + +    KKTYI+ +     PESF  H  WY S L S S    ++Y Y  
Sbjct: 11  ITIITTFLF-LLLHTTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNSES---SLLYTYTT 70

Query: 70  VVHGFSTRLTAEEAQR-LEGQPGILAVVPEMRYELHTTRTPQFLGLDKNADLYPESNSVS 129
             HGFS  L + EA   L     IL +  +  Y LHTTRTP+FLGL+    ++   +S +
Sbjct: 71  SFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSN 130

Query: 130 EVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARFFSKGYE 189
            VIIGVLDTGVWPES+SFDDT +  +PS WKGECESG++F +  CN+KLIGAR FSKG++
Sbjct: 131 GVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQ 190

Query: 190 -ATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARARVAAY 249
            A+ G     +ES SPRD DGHGTHT+TTAAGS V NAS  GYA+GTARGMA RARVA Y
Sbjct: 191 MASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATY 250

Query: 250 KVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKGILISC 309
           KVCW+ GCF SDILAA+++A+ D V+VLS+SLGGG + YY+D++A GAF+AME+G+ +SC
Sbjct: 251 KVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSC 310

Query: 310 SAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAFVSLGDGKNFSGVSLYRGKPLPGTLLP 369
           SAGN+GP+  S++N +PW+ TVGAGTLDRDFPAF +LG+GK  +GVSLY G  +    L 
Sbjct: 311 SAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLE 370

Query: 370 FIYAANASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVLANT 429
            +Y  N  N+S+ NLC+ G+L    V GKIV CDRGVN RV+KGAVV+ AGG+GM++ANT
Sbjct: 371 LVY--NKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANT 430

Query: 430 AANGEELVADAHLLPATAVGQKSGDMIRKYLISEPNPTVTILFEGTKLGIEPSPVVAAFS 489
           AA+GEELVAD+HLLPA AVG+K+GD++R+Y+ S+  PT  ++F+GT L ++PSPVVAAFS
Sbjct: 431 AASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFS 490

Query: 490 SRGPNSITPQLLKPDIIAPGVNIIAGWSKSVGPSGLPIDDRRVDFNIISGTSMSCPHVSG 549
           SRGPN++TP++LKPD+I PGVNI+AGWS ++GP+GL  D RR  FNI+SGTSMSCPH+SG
Sbjct: 491 SRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISG 550

Query: 550 LAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKLQDIATGKPSTPFDHGAGHVDPVSAL 609
           LA L+K AHP+WSP+AI+SALMTTAY        L D A    S P+ HG+GHVDP  AL
Sbjct: 551 LAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKAL 610

Query: 610 NPGLVYDLTVDDYLNFLCALNYTSSQINSLARREFTCDAGKKYS-VNDLNYPSFAAVFEG 669
           +PGLVYD++ ++Y+ FLC+L+YT   I ++ +R  + +  KK+S    LNYPSF+ +F  
Sbjct: 611 SPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRP-SVNCSKKFSDPGQLNYPSFSVLF-- 670

Query: 670 ALGGGSGVVKHTRTLTNVGSPGT-YKVSITSETKLVKISVEPESLTFAEANEKKSYTATF 729
              GG  VV++TR +TNVG+  + YKV++      V ISV+P  L+F    EKK YT TF
Sbjct: 671 ---GGKRVVRYTREVTNVGAASSVYKVTVNGAPS-VGISVKPSKLSFKSVGEKKRYTVTF 730

Query: 730 ATTTGSAAAPSTEGFGRIEWSDGKHVVGSPIAFSW 761
            +  G +     E FG I WS+ +H V SP+AFSW
Sbjct: 731 VSKKGVSMTNKAE-FGSITWSNPQHEVRSPVAFSW 751

BLAST of Sgr026245 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 776.5 bits (2004), Expect = 1.9e-224
Identity = 396/750 (52.80%), Postives = 517/750 (68.93%), Query Frame = 0

Query: 27  KKTYIVHMAKYQMPESFEHHLHWYDSSLKSVS---------DTAEMIYAYNNVVHGFSTR 86
           KKTY++HM K  MP  + +HL WY S + SV+         +   ++Y Y    HG + +
Sbjct: 34  KKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNNRILYTYQTAFHGLAAQ 93

Query: 87  LTAEEAQRLEGQPGILAVVPEMRYELHTTRTPQFLGLDKNAD--LYPESNSVSEVIIGVL 146
           LT EEA+RLE + G++AV+PE RYELHTTR+P FLGL++     ++ E  +  +V++GVL
Sbjct: 94  LTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVWAERVTDHDVVVGVL 153

Query: 147 DTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARFFSKGYEATLGPID 206
           DTG+WPES+SF+DTG+ PVP++W+G CE+G  F   NCNRK++GAR F +GYEA  G ID
Sbjct: 154 DTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGARVFYRGYEAATGKID 213

Query: 207 ESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARARVAAYKVCWAGGC 266
           E  E +SPRD DGHGTHTA T AGS V+ A+LFG+A GTARGMA +ARVAAYKVCW GGC
Sbjct: 214 EELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVAAYKVCWVGGC 273

Query: 267 FSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKGILISCSAGNAGPS 326
           FSSDIL+A+++AV D V VLS+SLGGGVS Y +DS++   F AME G+ +SCSAGN GP 
Sbjct: 274 FSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPD 333

Query: 327 PYSLSNTSPWITTVGAGTLDRDFPAFVSLGDGKNFSGVSLYRGKPL--PGTLLPFIY-AA 386
           P SL+N SPWITTVGA T+DRDFPA V +G  + F GVSLY+G+ +       P +Y   
Sbjct: 334 PISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYLGR 393

Query: 387 NASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVLANTAANGE 446
           NAS+    + C+ G L    VAGKIV CDRGV PRVQKG VVK AGGIGMVL NTA NGE
Sbjct: 394 NASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGE 453

Query: 447 ELVADAHLLPATAVGQKSGDMIRKYLISEPNPTVTILFEGTKLGIEPSPVVAAFSSRGPN 506
           ELVAD+H+LPA AVG+K G +I++Y ++    T ++   GT++GI+PSPVVAAFSSRGPN
Sbjct: 454 ELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGIKPSPVVAAFSSRGPN 513

Query: 507 SITPQLLKPDIIAPGVNIIAGWSKSVGPSGLPIDDRRVDFNIISGTSMSCPHVSGLAALI 566
            ++ ++LKPD++APGVNI+A W+  + PS L  D RRV FNI+SGTSMSCPHVSG+AALI
Sbjct: 514 FLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGVAALI 573

Query: 567 KGAHPDWSPAAIRSALMTTAYTAYKNGQKLQDIATGKPSTPFDHGAGHVDPVSALNPGLV 626
           K  HPDWSPAAI+SALMTTAY      + L D +   PS+P+DHGAGH+DP+ A +PGLV
Sbjct: 574 KSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLV 633

Query: 627 YDLTVDDYLNFLCALNYTSSQINSLARR-EFTCDAGKKYSVNDLNYPSFAAVFEGALGGG 686
           YD+   +Y  FLC  + + SQ+    +    TC      +  +LNYP+ +A+F       
Sbjct: 634 YDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNYPAISALFPENTHVK 693

Query: 687 SGVVKHTRTLTNVGSP-GTYKVSITSETKLVKISVEPESLTFAEANEKKSYTATFATTTG 746
           +  ++  RT+TNVG    +YKVS+ S  K   ++V+P++L F   ++K SYT TF T   
Sbjct: 694 AMTLR--RTVTNVGPHISSYKVSV-SPFKGASVTVQPKTLNFTSKHQKLSYTVTFRT--- 753

Query: 747 SAAAPSTEGFGRIEWSDGKHVVGSPIAFSW 761
                    FG + W    H V SP+  +W
Sbjct: 754 -RFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Sgr026245 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 769.2 bits (1985), Expect = 3.0e-222
Identity = 402/764 (52.62%), Postives = 519/764 (67.93%), Query Frame = 0

Query: 12  VLLCFVFGVSMAAAEKKTYIVHMAKYQMPESFEHHLHWYDSSLKSVSDT---AEMIYAYN 71
           +LLCF    S ++   ++YIVH+ +   P  F  H +W+ S L+S+  +   A ++Y+Y+
Sbjct: 15  LLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQPATLLYSYS 74

Query: 72  NVVHGFSTRLTAEEAQRLEGQPGILAVVPEMRYELHTTRTPQFLGLDKNADLYPESNSVS 131
             VHGFS RL+  +   L   P +++V+P+   E+HTT TP FLG  +N+ L+  SN   
Sbjct: 75  RAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYGE 134

Query: 132 EVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARFFSKGYE 191
           +VI+GVLDTG+WPE  SF D+GLGP+PS+WKGECE G +F AS+CNRKLIGAR F +GY 
Sbjct: 135 DVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYL 194

Query: 192 ATLGPIDE--SKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARARVAA 251
                  +  +KESRSPRD +GHGTHTA+TAAGSVV NASL+ YA GTA GMA++AR+AA
Sbjct: 195 TQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAA 254

Query: 252 YKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLG--GGVSDYYKDSVATGAFAAMEKGIL 311
           YK+CW GGC+ SDILAA+++AV D V+V+S+S+G  G   +Y+ DS+A GAF A   GI+
Sbjct: 255 YKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIV 314

Query: 312 ISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAFVSLGDGKNFSGVSLYRGKPLPGT 371
           +SCSAGN+GP+P + +N +PWI TVGA T+DR+F A    GDGK F+G SLY G+ LP +
Sbjct: 315 VSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDS 374

Query: 372 LLPFIYAANASNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVL 431
            L  +Y+ +  +     LC  G L    V GKIV CDRG N RV+KG+ VK AGG GM+L
Sbjct: 375 QLSLVYSGDCGS----RLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGGAGMIL 434

Query: 432 ANTAANGEELVADAHLLPATAVGQKSGDMIRKYLISEPNPTVTILFEGTKLG-IEPSPVV 491
           ANTA +GEEL AD+HL+PAT VG K+GD IR Y+ +  +PT  I F GT +G   PSP V
Sbjct: 435 ANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSPRV 494

Query: 492 AAFSSRGPNSITPQLLKPDIIAPGVNIIAGWSKSVGPSGLPIDDRRVDFNIISGTSMSCP 551
           AAFSSRGPN +TP +LKPD+IAPGVNI+AGW+  VGP+ L ID RRV FNIISGTSMSCP
Sbjct: 495 AAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGTSMSCP 554

Query: 552 HVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKLQDIATGKPSTPFDHGAGHVDP 611
           HVSGLAAL++ AHPDWSPAAI+SAL+TTAY    +G+ ++D+ATGK S  F HGAGHVDP
Sbjct: 555 HVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHVDP 614

Query: 612 VSALNPGLVYDLTVDDYLNFLCALNYTSSQINSLARREF---TCDAGKKYSVNDLNYPSF 671
             ALNPGLVYD+ V +Y+ FLCA+ Y    I    +       C+  K  +  DLNYPSF
Sbjct: 615 NKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPSF 674

Query: 672 AAVFEGALGGGSGVVKHTRTLTNVGS--PGTYKVSITSETKLVKISVEPESLTFAEANEK 731
           + VF         VVK+ R + NVGS     Y+V + S    V+I V P  L F++    
Sbjct: 675 SVVFAST----GEVVKYKRVVKNVGSNVDAVYEVGVKSPAN-VEIDVSPSKLAFSKEKSV 734

Query: 732 KSYTATFATTTGSAAAPSTEG--FGRIEWSDGKHVVGSPIAFSW 761
             Y  TF +        S  G  FG IEW+DG+HVV SP+A  W
Sbjct: 735 LEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of Sgr026245 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 759.6 bits (1960), Expect = 2.4e-219
Identity = 391/751 (52.06%), Postives = 516/751 (68.71%), Query Frame = 0

Query: 21  SMAAAEKKTYIVHMAKYQMPESFEHHLHWYDSSLKSV-SDTAEMIYAYNNVVHGFSTRLT 80
           S +++   TYIVH+     P  F  H HWY SSL S+ S    +I+ Y+ V HGFS RLT
Sbjct: 19  SASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPPSIIHTYDTVFHGFSARLT 78

Query: 81  AEEAQRLEGQPGILAVVPEMRYELHTTRTPQFLGL--DKNADLYPESNSVSEVIIGVLDT 140
           +++A +L   P +++V+PE    LHTTR+P+FLGL     A L  ES+  S+++IGV+DT
Sbjct: 79  SQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVIDT 138

Query: 141 GVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARFFSKGYEATLGPIDES 200
           GVWPE  SFDD GLGPVP  WKG+C +  +F  S CNRKL+GARFF  GYEAT G ++E+
Sbjct: 139 GVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNET 198

Query: 201 KESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARARVAAYKVCWAGGCFS 260
            E RSPRD DGHGTHTA+ +AG  V  AS  GYA G A GMA +AR+AAYKVCW  GC+ 
Sbjct: 199 TEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYD 258

Query: 261 SDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKGILISCSAGNAGPSPY 320
           SDILAA + AV D V+V+S+S+GG V  YY D++A GAF A+++GI +S SAGN GP   
Sbjct: 259 SDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGAL 318

Query: 321 SLSNTSPWITTVGAGTLDRDFPAFVSLGDGKNFSGVSLYRGKPL-PGTLLPFIYAAN--A 380
           +++N +PW+TTVGAGT+DRDFPA V LG+GK  SGVS+Y G  L PG + P +Y  +   
Sbjct: 319 TVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSLLG 378

Query: 381 SNASNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVLANTAANGEEL 440
            +  + +LC+ G+L P  V GKIV CDRG+N R  KG +V+  GG+GM++AN   +GE L
Sbjct: 379 GDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGL 438

Query: 441 VADAHLLPATAVGQKSGDMIRKYLI------SEPNPTVTILFEGTKLGIEPSPVVAAFSS 500
           VAD H+LPAT+VG   GD IR+Y+       S  +PT TI+F+GT+LGI P+PVVA+FS+
Sbjct: 439 VADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSA 498

Query: 501 RGPNSITPQLLKPDIIAPGVNIIAGWSKSVGPSGLPIDDRRVDFNIISGTSMSCPHVSGL 560
           RGPN  TP++LKPD+IAPG+NI+A W   +GPSG+  D+RR +FNI+SGTSM+CPHVSGL
Sbjct: 499 RGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGL 558

Query: 561 AALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKLQDIATGKPSTPFDHGAGHVDPVSALN 620
           AAL+K AHPDWSPAAIRSAL+TTAYT   +G+ + D +TG  S+  D+G+GHV P  A++
Sbjct: 559 AALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMD 618

Query: 621 PGLVYDLTVDDYLNFLCALNYTSSQINSLARREFTCDAGKKYS-VNDLNYPSFAAVFEGA 680
           PGLVYD+T  DY+NFLC  NYT + I ++ RR+  CD  ++   V +LNYPSF+ VF+  
Sbjct: 619 PGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQ-Q 678

Query: 681 LGGGSGVVKHTRTLTNVG-SPGTYKVSITSETKLVKISVEPESLTFAEANEKKSYTATFA 740
            G         RT+TNVG S   Y++ I    +   ++VEPE L+F    +K S+     
Sbjct: 679 YGESKMSTHFIRTVTNVGDSDSVYEIKI-RPPRGTTVTVEPEKLSFRRVGQKLSFVVRVK 738

Query: 741 TTTGSAAAPSTE-GFGRIEWSDGKHVVGSPI 757
           TT    +  +T    G I WSDGK  V SP+
Sbjct: 739 TTEVKLSPGATNVETGHIVWSDGKRNVTSPL 767